Protein ROOT HAIR DEFECTIVE 3 homolog
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 911 |
| Acc65I | GGTACC | 1 cut(s) | 1838 |
| AccB1I | GGYRCC | 3 cut(s) | 396, 1122, 1838 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 968 |
| AccBSI | CCGCTC | 1 cut(s) | 616 |
| AccI | GTMKAC | 2 cut(s) | 1029, 1988 |
| AccII | CGCG | 1 cut(s) | 510 |
| AccIII | TCCGGA | 1 cut(s) | 773 |
| AciI | CCGC | 9 cut(s) | 5, 8, 60, 105, 150, 614, 703, 1842, 2186 |
| AclWI | GGATC | 3 cut(s) | 840, 1647, 1877 |
| AcsI | RAATTY | 5 cut(s) | 623, 1147, 1235, 1561, 2093 |
| AcuI | CTGAAG | 1 cut(s) | 2325 |
| AcyI | GRCGYC | 2 cut(s) | 908, 2293 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 135, 773, 962, 968, 1119, 1636, 1722 |
| AflIII | ACRYGT | 1 cut(s) | 390 |
| AjiI | CACGTC | 1 cut(s) | 1313 |
| AjnI | CCWGG | 5 cut(s) | 730, 828, 961, 1445, 1721 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1079, 1111 |
| Alw21I | GWGCWC | 4 cut(s) | 352, 793, 2209, 2384 |
| Alw26I | GTCTC | 3 cut(s) | 1472, 1957, 2260 |
| Alw44I | GTGCAC | 1 cut(s) | 789 |
| AlwI | GGATC | 3 cut(s) | 840, 1647, 1877 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 1259, 1982, 2390 |
| Ama87I | CYCGRG | 1 cut(s) | 2443 |
| Aor13HI | TCCGGA | 1 cut(s) | 773 |
| AoxI | GGCC | 4 cut(s) | 137, 1023, 2058, 2160 |
| ApaLI | GTGCAC | 1 cut(s) | 789 |
| ApeKI | GCWGC | 6 cut(s) | 28, 422, 706, 1259, 1736, 1766 |
| ApoI | RAATTY | 5 cut(s) | 623, 1147, 1235, 1561, 2093 |
| Asp718I | GGTACC | 1 cut(s) | 1838 |
| AspA2I | CCTAGG | 1 cut(s) | 1682 |
| AspLEI | GCGC | 2 cut(s) | 468, 2311 |
| AspS9I | GGNCC | 5 cut(s) | 70, 137, 826, 953, 2058 |
| AsuC2I | CCSGG | 1 cut(s) | 64 |
| AsuHPI | GGTGA | 1 cut(s) | 758 |
| AsuII | TTCGAA | 1 cut(s) | 1207 |
| AvaI | CYCGRG | 1 cut(s) | 2443 |
| AvaII | GGWCC | 3 cut(s) | 70, 826, 953 |
| AvrII | CCTAGG | 1 cut(s) | 1682 |
| BaeGI | GKGCMC | 2 cut(s) | 793, 1125 |
| BanI | GGYRCC | 3 cut(s) | 396, 1122, 1838 |
| BanII | GRGCYC | 4 cut(s) | 111, 1420, 2384, 2450 |
| BbsI | GAAGAC | 3 cut(s) | 1446, 1648, 1710 |
| Bbv12I | GWGCWC | 4 cut(s) | 352, 793, 2209, 2384 |
| BbvCI | CCTCAGC | 1 cut(s) | 2361 |
| BbvI | GCAGC | 6 cut(s) | 40, 409, 718, 1246, 1723, 1753 |
| BccI | CCATC | 6 cut(s) | 193, 296, 995, 1823, 2170, 2482 |
| BceAI | ACGGC | 5 cut(s) | 55, 61, 992, 1010, 2285 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1537, 1571 |
| BciT130I | CCWGG | 5 cut(s) | 732, 830, 963, 1447, 1723 |
| BciVI | GTATCC | 2 cut(s) | 1276, 1918 |
| BcnI | CCSGG | 1 cut(s) | 64 |
| BcoDI | GTCTC | 3 cut(s) | 1472, 1957, 2260 |
| BfaI | CTAG | 2 cut(s) | 992, 1683 |
| BfmI | CTRYAG | 4 cut(s) | 120, 276, 633, 1488 |
| BfoI | RGCGCY | 1 cut(s) | 2312 |
| BfuI | GTATCC | 2 cut(s) | 1276, 1918 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2066 |
| BisI | GCNGC | 9 cut(s) | 6, 29, 60, 423, 704, 707, 1260, 1737, 1767 |
| BlnI | CCTAGG | 1 cut(s) | 1682 |
| BlpI | GCTNAGC | 1 cut(s) | 980 |
| BlsI | GCNGC | 9 cut(s) | 7, 30, 61, 424, 705, 708, 1261, 1738, 1768 |
| BmcAI | AGTACT | 1 cut(s) | 160 |
| Bme1390I | CCNGG | 6 cut(s) | 64, 732, 830, 963, 1447, 1723 |
| Bme18I | GGWCC | 3 cut(s) | 70, 826, 953 |
| BmeT110I | CYCGRG | 1 cut(s) | 2443 |
| BmgBI | CACGTC | 1 cut(s) | 1313 |
| BmgT120I | GGNCC | 5 cut(s) | 70, 137, 826, 953, 2058 |
| BmiI | GGNNCC | 8 cut(s) | 138, 221, 398, 543, 827, 1124, 1840, 2114 |
| BmrFI | CCNGG | 6 cut(s) | 64, 732, 830, 963, 1447, 1723 |
| BmrI | ACTGGG | 1 cut(s) | 1704 |
| BmsI | GCATC | 7 cut(s) | 193, 453, 1214, 1246, 1312, 1340, 1717 |
| BmuI | ACTGGG | 1 cut(s) | 1704 |
| BpiI | GAAGAC | 3 cut(s) | 1446, 1648, 1710 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1290, 1322 |
| BpmI | CTGGAG | 3 cut(s) | 560, 714, 1452 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2361 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 980 |
| Bpu14I | TTCGAA | 1 cut(s) | 1207 |
| BpuEI | CTTGAG | 4 cut(s) | 1039, 1730, 1983, 2271 |
| BpuMI | CCSGG | 1 cut(s) | 64 |
| Bsa29I | ATCGAT | 1 cut(s) | 99 |
| BsaAI | YACGTR | 1 cut(s) | 482 |
| BsaHI | GRCGYC | 2 cut(s) | 908, 2293 |
| BsaJI | CCNNGG | 5 cut(s) | 1243, 1446, 1682, 1722, 2054 |
| BsaWI | WCCGGW | 1 cut(s) | 773 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 662, 692 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 135, 773, 962, 968, 1119, 1636, 1722 |
| Bse1I | ACTGG | 8 cut(s) | 654, 956, 1699, 1840, 1940, 2045, 2321, 2372 |
| Bse3DI | GCAATG | 3 cut(s) | 1952, 2076, 2181 |
| BseAI | TCCGGA | 1 cut(s) | 773 |
| BseBI | CCWGG | 5 cut(s) | 732, 830, 963, 1447, 1723 |
| BseCI | ATCGAT | 1 cut(s) | 99 |
| BseDI | CCNNGG | 5 cut(s) | 1243, 1446, 1682, 1722, 2054 |
| BseGI | GGATG | 9 cut(s) | 208, 328, 987, 1229, 1253, 1732, 1786, 1834, 2396 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 135, 773, 962, 968, 1119, 1636, 1722 |
| BseMI | GCAATG | 3 cut(s) | 1952, 2076, 2181 |
| BseMII | CTCAG | 9 cut(s) | 155, 631, 994, 1404, 1974, 2255, 2375, 2382, 2397 |
| BseNI | ACTGG | 8 cut(s) | 654, 956, 1699, 1840, 1940, 2045, 2321, 2372 |
| BseRI | GAGGAG | 5 cut(s) | 686, 701, 1449, 1922, 2040 |
| BseSI | GKGCMC | 2 cut(s) | 793, 1125 |
| BseXI | GCAGC | 6 cut(s) | 40, 409, 718, 1246, 1723, 1753 |
| BseYI | CCCAGC | 1 cut(s) | 1613 |
| BsgI | GTGCAG | 1 cut(s) | 1272 |
| Bsh1236I | CGCG | 1 cut(s) | 510 |
| BshFI | GGCC | 4 cut(s) | 139, 1025, 2060, 2162 |
| BshNI | GGYRCC | 3 cut(s) | 396, 1122, 1838 |
| BshVI | ATCGAT | 1 cut(s) | 99 |
| BsiHKAI | GWGCWC | 4 cut(s) | 352, 793, 2209, 2384 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2443 |
| BsiSI | CCGG | 2 cut(s) | 63, 774 |
| BsiWI | CGTACG | 1 cut(s) | 114 |
| BslFI | GGGAC | 3 cut(s) | 749, 839, 1726 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 135, 773, 962, 968, 1119, 1636, 1722 |
| BsmAI | GTCTC | 3 cut(s) | 1472, 1957, 2260 |
| BsmBI | CGTCTC | 1 cut(s) | 2260 |
| BsmFI | GGGAC | 3 cut(s) | 749, 839, 1726 |
| BsnI | GGCC | 4 cut(s) | 139, 1025, 2060, 2162 |
| BsoBI | CYCGRG | 1 cut(s) | 2443 |
| Bsp119I | TTCGAA | 1 cut(s) | 1207 |
| Bsp1286I | GDGCHC | 8 cut(s) | 111, 352, 793, 1125, 1420, 2209, 2384, 2450 |
| Bsp13I | TCCGGA | 1 cut(s) | 773 |
| Bsp1407I | TGTACA | 2 cut(s) | 1214, 1264 |
| Bsp143I | GATC | 4 cut(s) | 33, 832, 1639, 1882 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 980 |
| BspACI | CCGC | 9 cut(s) | 5, 8, 60, 105, 150, 614, 703, 1842, 2186 |
| BspANI | GGCC | 4 cut(s) | 139, 1025, 2060, 2162 |
| BspCNI | CTCAG | 9 cut(s) | 154, 630, 993, 1403, 1975, 2256, 2374, 2383, 2396 |
| BspDI | ATCGAT | 1 cut(s) | 99 |
| BspEI | TCCGGA | 1 cut(s) | 773 |
| BspFNI | CGCG | 1 cut(s) | 510 |
| BspHI | TCATGA | 1 cut(s) | 459 |
| BspLI | GGNNCC | 8 cut(s) | 138, 221, 398, 543, 827, 1124, 1840, 2114 |
| BspPI | GGATC | 3 cut(s) | 840, 1647, 1877 |
| BspT104I | TTCGAA | 1 cut(s) | 1207 |
| BspT107I | GGYRCC | 3 cut(s) | 396, 1122, 1838 |
| BsrBI | CCGCTC | 1 cut(s) | 616 |
| BsrDI | GCAATG | 3 cut(s) | 1952, 2076, 2181 |
| BsrGI | TGTACA | 2 cut(s) | 1214, 1264 |
| BsrI | ACTGG | 8 cut(s) | 654, 956, 1699, 1840, 1940, 2045, 2321, 2372 |
| BssECI | CCNNGG | 5 cut(s) | 1243, 1446, 1682, 1722, 2054 |
| BssMI | GATC | 4 cut(s) | 33, 832, 1639, 1882 |
| BssNAI | GTATAC | 1 cut(s) | 1989 |
| BssNI | GRCGYC | 2 cut(s) | 908, 2293 |
| BssT1I | CCWWGG | 1 cut(s) | 1682 |
| Bst1107I | GTATAC | 1 cut(s) | 1989 |
| Bst2UI | CCWGG | 5 cut(s) | 732, 830, 963, 1447, 1723 |
| Bst6I | CTCTTC | 4 cut(s) | 149, 657, 870, 1602 |
| BstACI | GRCGYC | 2 cut(s) | 908, 2293 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1259 |
| BstAUI | TGTACA | 2 cut(s) | 1214, 1264 |
| BstBAI | YACGTR | 1 cut(s) | 482 |
| BstBI | TTCGAA | 1 cut(s) | 1207 |
| BstC8I | GCNNGC | 6 cut(s) | 107, 420, 741, 1229, 1272, 2274 |
| BstDSI | CCRYGG | 2 cut(s) | 1243, 2054 |
| BstF5I | GGATG | 9 cut(s) | 208, 328, 987, 1229, 1253, 1732, 1786, 1834, 2396 |
| BstFNI | CGCG | 1 cut(s) | 510 |
| BstH2I | RGCGCY | 1 cut(s) | 2312 |
| BstHHI | GCGC | 2 cut(s) | 468, 2311 |
| BstKTI | GATC | 4 cut(s) | 36, 835, 1642, 1885 |
| BstMAI | GTCTC | 3 cut(s) | 1472, 1957, 2260 |
| BstMBI | GATC | 4 cut(s) | 33, 832, 1639, 1882 |
| BstNI | CCWGG | 5 cut(s) | 732, 830, 963, 1447, 1723 |
| BstNSI | RCATGY | 3 cut(s) | 394, 1231, 1331 |
| BstSCI | CCNGG | 6 cut(s) | 62, 730, 828, 961, 1445, 1721 |
| BstSFI | CTRYAG | 4 cut(s) | 120, 276, 633, 1488 |
| BstSLI | GKGCMC | 2 cut(s) | 793, 1125 |
| BstUI | CGCG | 1 cut(s) | 510 |
| BstV1I | GCAGC | 6 cut(s) | 40, 409, 718, 1246, 1723, 1753 |
| BstV2I | GAAGAC | 3 cut(s) | 1446, 1648, 1710 |
| BstX2I | RGATCY | 2 cut(s) | 832, 1882 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 407, 2254 |
| BstYI | RGATCY | 2 cut(s) | 832, 1882 |
| BstZ17I | GTATAC | 1 cut(s) | 1989 |
| Bsu15I | ATCGAT | 1 cut(s) | 99 |
| BsuI | GTATCC | 2 cut(s) | 1276, 1918 |
| BsuRI | GGCC | 4 cut(s) | 139, 1025, 2060, 2162 |
| BsuTUI | ATCGAT | 1 cut(s) | 99 |
| BtgI | CCRYGG | 2 cut(s) | 1243, 2054 |
| BtrI | CACGTC | 1 cut(s) | 1313 |
| BtsCI | GGATG | 9 cut(s) | 208, 328, 987, 1229, 1253, 1732, 1786, 1834, 2396 |
| BtsI | GCAGTG | 1 cut(s) | 1107 |
| BtsIMutI | CAGTG | 7 cut(s) | 625, 630, 1107, 1675, 1947, 1955, 2247 |
| Cac8I | GCNNGC | 6 cut(s) | 107, 420, 741, 1229, 1272, 2274 |
| CaiI | CAGNNNCTG | 3 cut(s) | 1259, 1982, 2390 |
| CciI | TCATGA | 1 cut(s) | 459 |
| CfoI | GCGC | 2 cut(s) | 468, 2311 |
| Cfr13I | GGNCC | 5 cut(s) | 70, 137, 826, 953, 2058 |
| ClaI | ATCGAT | 1 cut(s) | 99 |
| CseI | GACGC | 3 cut(s) | 1728, 2282, 2467 |
| CsiI | ACCWGGT | 1 cut(s) | 961 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1566, 1601 |
| DpnI | GATC | 4 cut(s) | 35, 834, 1641, 1884 |
| DpnII | GATC | 4 cut(s) | 33, 832, 1639, 1882 |
| DraI | TTTAAA | 1 cut(s) | 1153 |
| Eam1104I | CTCTTC | 4 cut(s) | 149, 657, 870, 1602 |
| EarI | CTCTTC | 4 cut(s) | 149, 657, 870, 1602 |
| EciI | GGCGGA | 1 cut(s) | 23 |
| Ecl136II | GAGCTC | 1 cut(s) | 2382 |
| Eco130I | CCWWGG | 1 cut(s) | 1682 |
| Eco147I | AGGCCT | 1 cut(s) | 2162 |
| Eco24I | GRGCYC | 4 cut(s) | 111, 1420, 2384, 2450 |
| Eco32I | GATATC | 1 cut(s) | 2260 |
| Eco47I | GGWCC | 3 cut(s) | 70, 826, 953 |
| Eco53kI | GAGCTC | 1 cut(s) | 2382 |
| Eco57I | CTGAAG | 1 cut(s) | 2325 |
| Eco88I | CYCGRG | 1 cut(s) | 2443 |
| EcoICRI | GAGCTC | 1 cut(s) | 2382 |
| EcoO109I | RGGNCCY | 2 cut(s) | 137, 826 |
| EcoRII | CCWGG | 5 cut(s) | 730, 828, 961, 1445, 1721 |
| EcoRV | GATATC | 1 cut(s) | 2260 |
| EcoT14I | CCWWGG | 1 cut(s) | 1682 |
| EcoT22I | ATGCAT | 3 cut(s) | 1141, 1229, 1333 |
| EcoT38I | GRGCYC | 4 cut(s) | 111, 1420, 2384, 2450 |
| ErhI | CCWWGG | 1 cut(s) | 1682 |
| Esp3I | CGTCTC | 1 cut(s) | 2260 |
| FaqI | GGGAC | 3 cut(s) | 749, 839, 1726 |
| FauI | CCCGC | 4 cut(s) | 98, 143, 621, 2179 |
| FblI | GTMKAC | 2 cut(s) | 1029, 1988 |
| Fnu4HI | GCNGC | 9 cut(s) | 6, 29, 60, 423, 704, 707, 1260, 1737, 1767 |
| FokI | GGATG | 9 cut(s) | 215, 335, 974, 1236, 1260, 1739, 1793, 1841, 2383 |
| FriOI | GRGCYC | 4 cut(s) | 111, 1420, 2384, 2450 |
| Fsp4HI | GCNGC | 9 cut(s) | 6, 29, 60, 423, 704, 707, 1260, 1737, 1767 |
| FspBI | CTAG | 2 cut(s) | 992, 1683 |
| GlaI | GCGC | 2 cut(s) | 467, 2310 |
| GluI | GCNGC | 9 cut(s) | 6, 29, 60, 423, 704, 707, 1260, 1737, 1767 |
| GsaI | CCCAGC | 1 cut(s) | 1617 |
| GsuI | CTGGAG | 3 cut(s) | 560, 714, 1452 |
| HaeII | RGCGCY | 1 cut(s) | 2312 |
| HaeIII | GGCC | 4 cut(s) | 139, 1025, 2060, 2162 |
| HapII | CCGG | 2 cut(s) | 63, 774 |
| HgaI | GACGC | 3 cut(s) | 1728, 2282, 2467 |
| HhaI | GCGC | 2 cut(s) | 468, 2311 |
| Hin1I | GRCGYC | 2 cut(s) | 908, 2293 |
| Hin6I | GCGC | 2 cut(s) | 466, 2309 |
| HinP1I | GCGC | 2 cut(s) | 466, 2309 |
| HinfI | GANTC | 7 cut(s) | 75, 169, 578, 1484, 1673, 2074, 2393 |
| HpaII | CCGG | 2 cut(s) | 63, 774 |
| HphI | GGTGA | 1 cut(s) | 758 |
| Hpy166II | GTNNAC | 5 cut(s) | 791, 974, 1030, 1107, 1989 |
| Hpy188I | TCNGA | 9 cut(s) | 144, 373, 1009, 1310, 1360, 1498, 1978, 2198, 2425 |
| Hpy8I | GTNNAC | 5 cut(s) | 791, 974, 1030, 1107, 1989 |
| Hpy99I | CGWCG | 5 cut(s) | 41, 47, 232, 493, 1314 |
| HpyCH4IV | ACGT | 5 cut(s) | 481, 491, 908, 1129, 1312 |
| HpySE526I | ACGT | 5 cut(s) | 481, 491, 908, 1129, 1312 |
| Hsp92I | GRCGYC | 2 cut(s) | 908, 2293 |
| HspAI | GCGC | 2 cut(s) | 466, 2309 |
| Kpn2I | TCCGGA | 1 cut(s) | 773 |
| KpnI | GGTACC | 1 cut(s) | 1842 |
| Kzo9I | GATC | 4 cut(s) | 33, 832, 1639, 1882 |
| LmnI | GCTCC | 8 cut(s) | 219, 415, 541, 989, 1165, 1311, 1632, 2106 |
| Lsp1109I | GCAGC | 6 cut(s) | 40, 409, 718, 1246, 1723, 1753 |
| LweI | GCATC | 7 cut(s) | 193, 453, 1214, 1246, 1312, 1340, 1717 |
| MabI | ACCWGGT | 1 cut(s) | 961 |
| MaeI | CTAG | 2 cut(s) | 992, 1683 |
| MaeII | ACGT | 5 cut(s) | 481, 491, 908, 1129, 1312 |
| MaeIII | GTNAC | 3 cut(s) | 1993, 2033, 2374 |
| MalI | GATC | 4 cut(s) | 35, 834, 1641, 1884 |
| MbiI | CCGCTC | 1 cut(s) | 616 |
| MboI | GATC | 4 cut(s) | 33, 832, 1639, 1882 |
| MfeI | CAATTG | 2 cut(s) | 1067, 1542 |
| MflI | RGATCY | 2 cut(s) | 832, 1882 |
| MhlI | GDGCHC | 8 cut(s) | 111, 352, 793, 1125, 1420, 2209, 2384, 2450 |
| MlyI | GAGTC | 1 cut(s) | 2402 |
| MmeI | TCCRAC | 4 cut(s) | 1333, 1509, 2221, 2321 |
| Mph1103I | ATGCAT | 3 cut(s) | 1141, 1229, 1333 |
| MroI | TCCGGA | 1 cut(s) | 773 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1112, 2252 |
| MspA1I | CMGCKG | 1 cut(s) | 31 |
| MspI | CCGG | 2 cut(s) | 63, 774 |
| MspR9I | CCNGG | 6 cut(s) | 64, 732, 830, 963, 1447, 1723 |
| MunI | CAATTG | 2 cut(s) | 1067, 1542 |
| MvaI | CCWGG | 5 cut(s) | 732, 830, 963, 1447, 1723 |
| MvnI | CGCG | 1 cut(s) | 510 |
| NciI | CCSGG | 1 cut(s) | 64 |
| NdeII | GATC | 4 cut(s) | 33, 832, 1639, 1882 |
| NlaIV | GGNNCC | 8 cut(s) | 138, 221, 398, 543, 827, 1124, 1840, 2114 |
| NmuCI | GTSAC | 1 cut(s) | 1993 |
| NsiI | ATGCAT | 3 cut(s) | 1141, 1229, 1333 |
| NspI | RCATGY | 3 cut(s) | 394, 1231, 1331 |
| NspV | TTCGAA | 1 cut(s) | 1207 |
| PaeI | GCATGC | 1 cut(s) | 1231 |
| PaeR7I | CTCGAG | 1 cut(s) | 2443 |
| PagI | TCATGA | 1 cut(s) | 459 |
| PceI | AGGCCT | 1 cut(s) | 2162 |
| PciI | ACATGT | 1 cut(s) | 390 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 45, 1859 |
| PfeI | GAWTC | 6 cut(s) | 75, 169, 578, 1484, 1673, 2074 |
| Pfl23II | CGTACG | 1 cut(s) | 114 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 968 |
| PfoI | TCCNGGA | 1 cut(s) | 730 |
| PkrI | GCNGC | 9 cut(s) | 7, 30, 61, 424, 705, 708, 1261, 1738, 1768 |
| PleI | GAGTC | 1 cut(s) | 2401 |
| PpsI | GAGTC | 1 cut(s) | 2401 |
| Ppu21I | YACGTR | 1 cut(s) | 482 |
| PpuMI | RGGWCCY | 1 cut(s) | 826 |
| PscI | ACATGT | 1 cut(s) | 390 |
| Psp124BI | GAGCTC | 1 cut(s) | 2384 |
| Psp5II | RGGWCCY | 1 cut(s) | 826 |
| Psp6I | CCWGG | 5 cut(s) | 730, 828, 961, 1445, 1721 |
| PspFI | CCCAGC | 1 cut(s) | 1613 |
| PspGI | CCWGG | 5 cut(s) | 730, 828, 961, 1445, 1721 |
| PspLI | CGTACG | 1 cut(s) | 114 |
| PspN4I | GGNNCC | 8 cut(s) | 138, 221, 398, 543, 827, 1124, 1840, 2114 |
| PspPI | GGNCC | 5 cut(s) | 70, 137, 826, 953, 2058 |
| PspPPI | RGGWCCY | 1 cut(s) | 826 |
| PspXI | VCTCGAGB | 1 cut(s) | 2443 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2105, 2137 |
| PstNI | CAGNNNCTG | 3 cut(s) | 1259, 1982, 2390 |
| PsuI | RGATCY | 2 cut(s) | 832, 1882 |
| PvuII | CAGCTG | 1 cut(s) | 31 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1112, 2252 |
| SacI | GAGCTC | 1 cut(s) | 2384 |
| SatI | GCNGC | 9 cut(s) | 6, 29, 60, 423, 704, 707, 1260, 1737, 1767 |
| Sau3AI | GATC | 4 cut(s) | 33, 832, 1639, 1882 |
| Sau96I | GGNCC | 5 cut(s) | 70, 137, 826, 953, 2058 |
| ScaI | AGTACT | 1 cut(s) | 160 |
| SchI | GAGTC | 1 cut(s) | 2402 |
| ScrFI | CCNGG | 6 cut(s) | 64, 732, 830, 963, 1447, 1723 |
| SduI | GDGCHC | 8 cut(s) | 111, 352, 793, 1125, 1420, 2209, 2384, 2450 |
| SexAI | ACCWGGT | 1 cut(s) | 961 |
| SfaNI | GCATC | 7 cut(s) | 193, 453, 1214, 1246, 1312, 1340, 1717 |
| SfcI | CTRYAG | 4 cut(s) | 120, 276, 633, 1488 |
| Sfr274I | CTCGAG | 1 cut(s) | 2443 |
| SfuI | TTCGAA | 1 cut(s) | 1207 |
| SinI | GGWCC | 3 cut(s) | 70, 826, 953 |
| SlaI | CTCGAG | 1 cut(s) | 2443 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1112, 2252 |
| SmlI | CTYRAG | 5 cut(s) | 1054, 1745, 1998, 2286, 2443 |
| SmoI | CTYRAG | 5 cut(s) | 1054, 1745, 1998, 2286, 2443 |
| SphI | GCATGC | 1 cut(s) | 1231 |
| SseBI | AGGCCT | 1 cut(s) | 2162 |
| SsiI | CCGC | 9 cut(s) | 5, 8, 60, 105, 150, 614, 703, 1842, 2186 |
| SspI | AATATT | 3 cut(s) | 1798, 2220, 2413 |
| SspMI | CTAG | 2 cut(s) | 992, 1683 |
| SstI | GAGCTC | 1 cut(s) | 2384 |
| StuI | AGGCCT | 1 cut(s) | 2162 |
| StyD4I | CCNGG | 6 cut(s) | 62, 730, 828, 961, 1445, 1721 |
| StyI | CCWWGG | 1 cut(s) | 1682 |
| TaiI | ACGT | 5 cut(s) | 484, 494, 911, 1132, 1315 |
| TaqI | TCGA | 7 cut(s) | 36, 99, 336, 1207, 1297, 2234, 2444 |
| TaqII | GACCGA | 1 cut(s) | 87 |
| TatI | WGTACW | 5 cut(s) | 158, 379, 1214, 1264, 1346 |
| TauI | GCSGC | 3 cut(s) | 8, 62, 706 |
| TfiI | GAWTC | 6 cut(s) | 75, 169, 578, 1484, 1673, 2074 |
| TscAI | CASTG | 7 cut(s) | 625, 637, 1114, 1675, 1947, 1962, 2254 |
| TseFI | GTSAC | 1 cut(s) | 1993 |
| TseI | GCWGC | 6 cut(s) | 28, 422, 706, 1259, 1736, 1766 |
| Tsp45I | GTSAC | 1 cut(s) | 1993 |
| TspDTI | ATGAA | 8 cut(s) | 182, 675, 771, 1053, 1649, 2106, 2220, 2406 |
| TspGWI | ACGGA | 3 cut(s) | 1197, 2043, 2389 |
| TspRI | CASTG | 7 cut(s) | 625, 637, 1114, 1675, 1947, 1962, 2254 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 968 |
| VneI | GTGCAC | 1 cut(s) | 789 |
| VpaK11BI | GGWCC | 3 cut(s) | 70, 826, 953 |
| XapI | RAATTY | 5 cut(s) | 623, 1147, 1235, 1561, 2093 |
| XceI | RCATGY | 3 cut(s) | 394, 1231, 1331 |
| XhoI | CTCGAG | 1 cut(s) | 2443 |
| XmaJI | CCTAGG | 1 cut(s) | 1682 |
| XmiI | GTMKAC | 2 cut(s) | 1029, 1988 |
| XspI | CTAG | 2 cut(s) | 992, 1683 |
| ZraI | GACGTC | 1 cut(s) | 909 |
| ZrmI | AGTACT | 1 cut(s) | 160 |
| Zsp2I | ATGCAT | 3 cut(s) | 1141, 1229, 1333 |