MD05G1257300.v1.1

Protein ROOT HAIR DEFECTIVE 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000525
Physical Location & Seq
Forward (+)
38825394 .. 38837264
11871 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1257300.v1.1.491

Sequence Viewer

Length: 1320 bp
ATGGAGACGCTATTGATATTGAGTAATCTTCGAAGAAAGTCACCTCTTCAGAGAAGTAATCTGGCAGCATTCGTTAAGCACTTTCCAAGGAATTCTCACGTTGCCTTGATTAAGTTATACATGGCCATGAGAAGTCTTCACAAGAAATCACTGCAAGAAGCTGTTGAAACTGGTCCCGTACAAGGCTTTGTTGTGTGTTTTCCATTCGTTGGAAGGTATGACATGGAGGCCATCTACTTTGATGAAGGTGTAAGGAACTCAAAACGACAACTGTTGGAGTCAAAAGCATTGGATTTTGTTTACCCTGCGTACTCGGCCATGCTCGGACACCTGCGTTCTAAAGCCCTTGAAGATTTTCAAGTGAGGCTGGAGCAGTCATTGAACAAAGGAGAAGGATTTGCTTCATCTGTTCGAACCTGTGCTCAGTCTTCTATGCTTGAGTTTGAGAAAGGATGTGCAGATGCTGCCATACAACAAGCTAATTGGGATGCTTCAAAAGCCCAGGAAAAGCTTCGTCGTGATATAGATGCACATGCATCATCGGTTCGTAGTGCAAAACTGGCAGAATTGAATTCCAACTATGAGAAAAAACTTTCTTCGTCTTTAAGTGGTCCTGTAGAAGCTCTACTCGAAACTGGTGCAAAAGACACCTGGGCTTTGATACGAAAACTACTTAATCGTGAGACTGAAGTTGCAGTATCAGAGTTCTCAACTGCAGTTGCCAATTTTGAGTTGGACTACAAAACGGTTGCCAAAATGAAGCAACATTTGAAGGATTATGCGAGAAATGTGGTGGAGACAAAAGCAAGAGAAGAGGCTGGGAAAATTATGATCCACATGAAGGATCGGTTTGCCGCAGTCTTCAATTATGACAGCGATTCAATGCCTAGGGTTTGGACTGGGAACGAAGACATTAAAAGTATTACCAAGGATGCACGAACTGCGTCTCTGAAGCTTTTGTCGACCATGGCTGCCATTCGCTTGGATGAGAAGCCAGATAATATTGAAAATGTCCTCGTTTCTTCTCTGGTGGACGGGACTGTTACTGTTTCATCTTCACAAAATAGGAAACTAGGACCTTCTACTGATCCTCTTGCCTCAAGCTCTTGGGAAGAGGTTTCTTCGAAGGATACCTTAATTACCCCAGTACAGTGCAAGTCATTGTGGAGGCAGTTCAAAGCAGAGACCGAATATAGCGTCACTCAAGCTATTTCGGCACAGGAGGCTCACAAGCGGAGTAACAACTGGTTACCTCCTCCATGGGCTATAATGGCGATGATTGTTCTTGGTTTTAACGAATTTATGATGCTTTTAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

440

Amino Acids

49.28

Weight (kDa)

8.9

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sey1_3HB PF20428 70 - 439 3e-124 Sey1 three-helix bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000525)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45160
fragaria_vesca FvH4_3g11890
malus_domestica MD00G1023300.v1.1 MD00G1023500.v1.1 MD00G1046300.v1.1 MD00G1046400.v1.1 MD00G1047200.v1.1 MD05G1180600.v1.1 MD05G1256900.v1.1 MD05G1257200.v1.1 MD05G1257300.v1.1 MD05G1257400.v1.1 MD05G1257900.v1.1 MD05G1258000.v1.1 MD10G1213100.v1.1 MD10G1237400.v1.1
prunus_persica Prupe.4G106400_v2.0.a1 Prupe.4G106400_v2.0.a1 Prupe.4G106900_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.4G113800_v2.0.a1 Prupe.I001700_v2.0.a1
pyrus_communis pycom05g23620 pycom05g23630 pycom05g23880 pycom05g24090 pycom05g24270 pycom10g19870
rosa_chinensis RchiOBHm_Chr5g0019311 RchiOBHm_Chr5g0019391 RchiOBHm_Chr5g0019401 RchiOBHm_Chr7g0226131 RchiOBHm_Chr7g0226141 RchiOBHm_Chr7g0226161
rosa_laevigata RLG00000001766 RLG00000001767 RLG00000032437 RLG00000032441 RLG00000032445
rosa_multiflora Rmu_sc0001350.1_g000022 Rmu_sc0001350.1_g000031 Rmu_sc0002141.1_g000028 Rmu_sc0002759.1_g000037 Rmu_sc0002759.1_g000039 Rmu_sc0005292.1_g000004 Rmu_sc0005292.1_g000050
rosa_roxburghii Rroxscaffold_1G00058650 Rroxscaffold_1G00058670 Rroxscaffold_1G00074270 Rroxscaffold_2G00108890 Rroxscaffold_2G00125570
rosa_rugosa Rorug05G0050100 Rorug05G0050200 Rorug05G0050300 Rorug05G0050500 Rorug05G0050600 Rorug05G0050700
rosa_samantha Rh5AG140700 Rh5AG141000 Rh5AG141300 Rh5AG390000 Rh5BG139700 Rh5BG139800 Rh5BG139900 Rh5BG140000 Rh5CG151000 Rh5CG151500 Rh5CG151600 Rh5DG140200 Rh5DG140400 Rh5DG140600 Rh7AG368100 Rh7AG381400 Rh7AG381700 Rh7CG386400 Rh7CG400900 Rh7CG401200 Rh7DG380400 Rh7DG380500
rosa_wichuraiana Rw5G012510 Rw5G012530 Rw5G012540 Rw7G031980 Rw7G031990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 339
Acc36I ACCTGC 1 cut(s) 339
AccB7I CCANNNNNTGG 1 cut(s) 209
AccI GTMKAC 1 cut(s) 962
AciI CCGC 2 cut(s) 855, 1234
AclWI GGATC 3 cut(s) 826, 852, 1082
AcoI YGGCCR 2 cut(s) 123, 315
AcsI RAATTY 3 cut(s) 91, 571, 1298
AcuI CTGAAG 3 cut(s) 32, 708, 971
AfaI GTAC 3 cut(s) 180, 311, 1149
AfiI CCNNNNNNNGG 3 cut(s) 182, 209, 841
AjnI CCWGG 2 cut(s) 501, 650
AluBI AGCT 7 cut(s) 161, 479, 511, 623, 955, 1104, 1208
AluI AGCT 7 cut(s) 161, 479, 511, 623, 955, 1104, 1208
Alw21I GWGCWC 1 cut(s) 424
Alw26I GTCTC 4 cut(s) 677, 791, 951, 1178
AlwI GGATC 3 cut(s) 826, 852, 1082
AlwNI CAGNNNCTG 1 cut(s) 464
AoxI GGCC 3 cut(s) 123, 228, 315
ApeKI GCWGC 3 cut(s) 65, 464, 971
ApoI RAATTY 3 cut(s) 91, 571, 1298
ArsI GACNNNNNNTTYG 2 cut(s) 1182, 1214
Asp700I GAANNNNTTC 2 cut(s) 354, 510
AspA2I CCTAGG 1 cut(s) 887
AspS9I GGNCC 3 cut(s) 173, 611, 1076
AsuHPI GGTGA 1 cut(s) 33
AsuII TTCGAA 3 cut(s) 31, 412, 1124
AvaII GGWCC 3 cut(s) 173, 611, 1076
AvrII CCTAGG 1 cut(s) 887
BalI TGGCCA 1 cut(s) 125
BbsI GAAGAC 4 cut(s) 128, 420, 853, 915
Bbv12I GWGCWC 1 cut(s) 424
BbvI GCAGC 3 cut(s) 77, 451, 958
BccI CCATC 1 cut(s) 239
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BciT130I CCWGG 2 cut(s) 503, 652
BciVI GTATCC 1 cut(s) 1123
BcoDI GTCTC 4 cut(s) 677, 791, 951, 1178
BfaI CTAG 2 cut(s) 888, 1073
BfmI CTRYAG 2 cut(s) 615, 714
BfuAI ACCTGC 1 cut(s) 339
BfuI GTATCC 1 cut(s) 1123
BisI GCNGC 4 cut(s) 66, 465, 855, 972
BlnI CCTAGG 1 cut(s) 887
BlsI GCNGC 4 cut(s) 67, 466, 856, 973
Bme1390I CCNGG 2 cut(s) 503, 652
Bme18I GGWCC 3 cut(s) 173, 611, 1076
BmgT120I GGNCC 3 cut(s) 173, 611, 1076
BmiI GGNNCC 1 cut(s) 175
BmrFI CCNGG 2 cut(s) 503, 652
BmrI ACTGGG 2 cut(s) 909, 1139
BmsI GCATC 6 cut(s) 451, 478, 517, 545, 922, 1296
BmuI ACTGGG 2 cut(s) 909, 1139
BpiI GAAGAC 4 cut(s) 128, 420, 853, 915
BpmI CTGGAG 1 cut(s) 389
Bpu14I TTCGAA 3 cut(s) 31, 412, 1124
BpuEI CTTGAG 3 cut(s) 458, 1084, 1188
BsaI GGTCTC 1 cut(s) 1178
BsaJI CCNNGG 7 cut(s) 86, 501, 651, 887, 927, 966, 1259
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 209, 841
Bse1I ACTGG 6 cut(s) 175, 564, 640, 904, 1145, 1250
BseBI CCWGG 2 cut(s) 503, 652
BseDI CCNNGG 7 cut(s) 86, 501, 651, 887, 927, 966, 1259
BseGI GGATG 4 cut(s) 458, 493, 937, 991
BseLI CCNNNNNNNGG 3 cut(s) 182, 209, 841
BseMII CTCAG 1 cut(s) 437
BseNI ACTGG 6 cut(s) 175, 564, 640, 904, 1145, 1250
BseRI GAGGAG 1 cut(s) 1245
BseXI GCAGC 3 cut(s) 77, 451, 958
BseYI CCCAGC 1 cut(s) 818
BsgI GTGCAG 1 cut(s) 477
BshFI GGCC 3 cut(s) 125, 230, 317
BsiHKAI GWGCWC 1 cut(s) 424
BslFI GGGAC 2 cut(s) 159, 1051
BslI CCNNNNNNNGG 3 cut(s) 182, 209, 841
BsmAI GTCTC 4 cut(s) 677, 791, 951, 1178
BsmBI CGTCTC 1 cut(s) 951
BsmFI GGGAC 2 cut(s) 159, 1051
BsmI GAATGC 1 cut(s) 68
BsnI GGCC 3 cut(s) 125, 230, 317
Bso31I GGTCTC 1 cut(s) 1178
Bsp119I TTCGAA 3 cut(s) 31, 412, 1124
Bsp1286I GDGCHC 1 cut(s) 424
Bsp143I GATC 3 cut(s) 831, 844, 1087
Bsp19I CCATGG 2 cut(s) 966, 1259
BspACI CCGC 2 cut(s) 855, 1234
BspANI GGCC 3 cut(s) 125, 230, 317
BspCNI CTCAG 1 cut(s) 436
BspLI GGNNCC 1 cut(s) 175
BspMAI CTGCAG 1 cut(s) 718
BspMI ACCTGC 1 cut(s) 339
BspPI GGATC 3 cut(s) 826, 852, 1082
BspT104I TTCGAA 3 cut(s) 31, 412, 1124
BspTNI GGTCTC 1 cut(s) 1178
BsrI ACTGG 6 cut(s) 175, 564, 640, 904, 1145, 1250
BssECI CCNNGG 7 cut(s) 86, 501, 651, 887, 927, 966, 1259
BssMI GATC 3 cut(s) 831, 844, 1087
BssT1I CCWWGG 5 cut(s) 86, 887, 927, 966, 1259
Bst2UI CCWGG 2 cut(s) 503, 652
Bst4CI ACNGT 5 cut(s) 273, 748, 1042, 1048, 1152
Bst6I CTCTTC 3 cut(s) 51, 807, 1107
BstAPI GCANNNNNTGC 2 cut(s) 464, 941
BstBI TTCGAA 3 cut(s) 31, 412, 1124
BstDEI CTNAG 1 cut(s) 423
BstDSI CCRYGG 2 cut(s) 966, 1259
BstEII GGTNACC 1 cut(s) 1248
BstF5I GGATG 4 cut(s) 458, 493, 937, 991
BstKTI GATC 3 cut(s) 834, 847, 1090
BstMAI GTCTC 4 cut(s) 677, 791, 951, 1178
BstMBI GATC 3 cut(s) 831, 844, 1087
BstMWI GCNNNNNNNGC 8 cut(s) 314, 464, 497, 560, 941, 1214, 1223, 1271
BstNI CCWGG 2 cut(s) 503, 652
BstNSI RCATGY 1 cut(s) 536
BstPI GGTNACC 1 cut(s) 1248
BstSCI CCNGG 2 cut(s) 501, 650
BstSFI CTRYAG 2 cut(s) 615, 714
BstV1I GCAGC 3 cut(s) 77, 451, 958
BstV2I GAAGAC 4 cut(s) 128, 420, 853, 915
BstXI CCANNNNNNTGG 1 cut(s) 982
BsuI GTATCC 1 cut(s) 1123
BsuRI GGCC 3 cut(s) 125, 230, 317
BtgI CCRYGG 2 cut(s) 966, 1259
BtgZI GCGATG 1 cut(s) 1289
BtsCI GGATG 4 cut(s) 458, 493, 937, 991
BtsI GCAGTG 1 cut(s) 149
BtsIMutI CAGTG 2 cut(s) 149, 1157
BveI ACCTGC 1 cut(s) 339
CaiI CAGNNNCTG 1 cut(s) 464
Cfr13I GGNCC 3 cut(s) 173, 611, 1076
CseI GACGC 3 cut(s) 16, 933, 1186
Csp6I GTAC 3 cut(s) 179, 310, 1148
CviAII CATG 8 cut(s) 121, 127, 223, 319, 533, 838, 967, 1260
CviQI GTAC 3 cut(s) 179, 310, 1148
DdeI CTNAG 1 cut(s) 423
DpnI GATC 3 cut(s) 833, 846, 1089
DpnII GATC 3 cut(s) 831, 844, 1087
DraI TTTAAA 1 cut(s) 1314
EaeI YGGCCR 2 cut(s) 123, 315
Eam1104I CTCTTC 3 cut(s) 51, 807, 1107
EarI CTCTTC 3 cut(s) 51, 807, 1107
Eco130I CCWWGG 5 cut(s) 86, 887, 927, 966, 1259
Eco31I GGTCTC 1 cut(s) 1178
Eco47I GGWCC 3 cut(s) 173, 611, 1076
Eco57I CTGAAG 3 cut(s) 32, 708, 971
Eco91I GGTNACC 1 cut(s) 1248
EcoO109I RGGNCCY 1 cut(s) 1076
EcoO65I GGTNACC 1 cut(s) 1248
EcoRI GAATTC 2 cut(s) 91, 571
EcoRII CCWGG 2 cut(s) 501, 650
EcoT14I CCWWGG 5 cut(s) 86, 887, 927, 966, 1259
EcoT22I ATGCAT 1 cut(s) 538
ErhI CCWWGG 5 cut(s) 86, 887, 927, 966, 1259
Esp3I CGTCTC 1 cut(s) 951
FaeI CATG 8 cut(s) 124, 130, 226, 322, 536, 841, 970, 1263
FalI AAGNNNNNCTT 2 cut(s) 1118, 1150
FaqI GGGAC 2 cut(s) 159, 1051
FatI CATG 8 cut(s) 120, 126, 222, 318, 532, 837, 966, 1259
FblI GTMKAC 1 cut(s) 962
Fnu4HI GCNGC 4 cut(s) 66, 465, 855, 972
FokI GGATG 4 cut(s) 465, 500, 944, 998
Fsp4HI GCNGC 4 cut(s) 66, 465, 855, 972
FspBI CTAG 2 cut(s) 888, 1073
GluI GCNGC 4 cut(s) 66, 465, 855, 972
GsaI CCCAGC 1 cut(s) 822
GsuI CTGGAG 1 cut(s) 389
HaeIII GGCC 3 cut(s) 125, 230, 317
HgaI GACGC 3 cut(s) 16, 933, 1186
Hin1II CATG 8 cut(s) 124, 130, 226, 322, 536, 841, 970, 1263
HincII GTYRAC 1 cut(s) 963
HindII GTYRAC 1 cut(s) 963
HindIII AAGCTT 2 cut(s) 509, 953
HinfI GANTC 2 cut(s) 278, 878
HphI GGTGA 1 cut(s) 33
Hpy166II GTNNAC 3 cut(s) 301, 963, 1033
Hpy188I TCNGA 4 cut(s) 51, 326, 703, 951
Hpy188III TCNNGA 2 cut(s) 518, 680
Hpy8I GTNNAC 3 cut(s) 301, 963, 1033
Hpy99I CGWCG 1 cut(s) 519
HpyAV CCTTC 7 cut(s) 207, 239, 386, 766, 835, 1089, 1120
HpyCH4III ACNGT 5 cut(s) 273, 748, 1042, 1048, 1152
HpyCH4IV ACGT 1 cut(s) 99
HpyF10VI GCNNNNNNNGC 8 cut(s) 314, 464, 497, 560, 941, 1214, 1223, 1271
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 8 cut(s) 124, 130, 226, 322, 536, 841, 970, 1263
Kzo9I GATC 3 cut(s) 831, 844, 1087
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 3 cut(s) 77, 451, 958
LweI GCATC 6 cut(s) 451, 478, 517, 545, 922, 1296
MaeI CTAG 2 cut(s) 888, 1073
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 5 cut(s) 39, 1042, 1198, 1238, 1248
MalI GATC 3 cut(s) 833, 846, 1089
MboI GATC 3 cut(s) 831, 844, 1087
MhlI GDGCHC 1 cut(s) 424
MlsI TGGCCA 1 cut(s) 125
MluCI AATT 9 cut(s) 91, 481, 566, 571, 724, 825, 865, 1137, 1298
MluNI TGGCCA 1 cut(s) 125
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 4 cut(s) 190, 255, 600, 714
Mox20I TGGCCA 1 cut(s) 125
Mph1103I ATGCAT 1 cut(s) 538
MroXI GAANNNNTTC 2 cut(s) 354, 510
MscI TGGCCA 1 cut(s) 125
MseI TTAA 8 cut(s) 75, 111, 605, 675, 915, 1136, 1293, 1313
MslI CAYNNNNRTG 1 cut(s) 125
Msp20I TGGCCA 1 cut(s) 125
MspR9I CCNGG 2 cut(s) 503, 652
Mva1269I GAATGC 1 cut(s) 68
MvaI CCWGG 2 cut(s) 503, 652
MwoI GCNNNNNNNGC 8 cut(s) 314, 464, 497, 560, 941, 1214, 1223, 1271
NcoI CCATGG 2 cut(s) 966, 1259
NdeII GATC 3 cut(s) 831, 844, 1087
NlaIII CATG 8 cut(s) 124, 130, 226, 322, 536, 841, 970, 1263
NlaIV GGNNCC 1 cut(s) 175
NmeAIII GCCGAG 1 cut(s) 293
NmuCI GTSAC 2 cut(s) 39, 1198
NsiI ATGCAT 1 cut(s) 538
NspI RCATGY 1 cut(s) 536
NspV TTCGAA 3 cut(s) 31, 412, 1124
PaqCI CACCTGC 1 cut(s) 339
PctI GAATGC 1 cut(s) 68
PdmI GAANNNNTTC 2 cut(s) 354, 510
PfeI GAWTC 1 cut(s) 878
PflMI CCANNNNNTGG 1 cut(s) 209
PkrI GCNGC 4 cut(s) 67, 466, 856, 973
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PpuMI RGGWCCY 1 cut(s) 1076
Psp5II RGGWCCY 1 cut(s) 1076
Psp6I CCWGG 2 cut(s) 501, 650
PspEI GGTNACC 1 cut(s) 1248
PspFI CCCAGC 1 cut(s) 818
PspGI CCWGG 2 cut(s) 501, 650
PspN4I GGNNCC 1 cut(s) 175
PspPI GGNCC 3 cut(s) 173, 611, 1076
PspPPI RGGWCCY 1 cut(s) 1076
PstI CTGCAG 1 cut(s) 718
PstNI CAGNNNCTG 1 cut(s) 464
RsaI GTAC 3 cut(s) 180, 311, 1149
RsaNI GTAC 3 cut(s) 179, 310, 1148
RseI CAYNNNNRTG 1 cut(s) 125
SalI GTCGAC 1 cut(s) 961
SaqAI TTAA 8 cut(s) 75, 111, 605, 675, 915, 1136, 1293, 1313
SatI GCNGC 4 cut(s) 66, 465, 855, 972
Sau3AI GATC 3 cut(s) 831, 844, 1087
Sau96I GGNCC 3 cut(s) 173, 611, 1076
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 2 cut(s) 503, 652
SduI GDGCHC 1 cut(s) 424
SfaNI GCATC 6 cut(s) 451, 478, 517, 545, 922, 1296
SfcI CTRYAG 2 cut(s) 615, 714
SfuI TTCGAA 3 cut(s) 31, 412, 1124
SinI GGWCC 3 cut(s) 173, 611, 1076
SmiMI CAYNNNNRTG 1 cut(s) 125
SmlI CTYRAG 3 cut(s) 437, 1099, 1203
SmoI CTYRAG 3 cut(s) 437, 1099, 1203
Sse9I AATT 9 cut(s) 91, 481, 566, 571, 724, 825, 865, 1137, 1298
SsiI CCGC 2 cut(s) 855, 1234
SspI AATATT 1 cut(s) 1003
SspMI CTAG 2 cut(s) 888, 1073
StyD4I CCNGG 2 cut(s) 501, 650
StyI CCWWGG 5 cut(s) 86, 887, 927, 966, 1259
TaaI ACNGT 5 cut(s) 273, 748, 1042, 1048, 1152
TaiI ACGT 1 cut(s) 102
TaqI TCGA 5 cut(s) 31, 412, 630, 962, 1124
TaqII GACCGA 1 cut(s) 1202
TasI AATT 9 cut(s) 91, 481, 566, 571, 724, 825, 865, 1137, 1298
TatI WGTACW 1 cut(s) 1147
TauI GCSGC 1 cut(s) 857
TfiI GAWTC 1 cut(s) 878
Tru1I TTAA 8 cut(s) 75, 111, 605, 675, 915, 1136, 1293, 1313
Tru9I TTAA 8 cut(s) 75, 111, 605, 675, 915, 1136, 1293, 1313
TscAI CASTG 2 cut(s) 156, 1157
TseFI GTSAC 2 cut(s) 39, 1198
TseI GCWGC 3 cut(s) 65, 464, 971
Tsp45I GTSAC 2 cut(s) 39, 1198
TspDTI ATGAA 5 cut(s) 258, 393, 773, 854, 1041
TspRI CASTG 2 cut(s) 156, 1157
Van91I CCANNNNNTGG 1 cut(s) 209
VpaK11BI GGWCC 3 cut(s) 173, 611, 1076
XapI RAATTY 3 cut(s) 91, 571, 1298
XceI RCATGY 1 cut(s) 536
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
XmaJI CCTAGG 1 cut(s) 887
XmiI GTMKAC 1 cut(s) 962
XmnI GAANNNNTTC 2 cut(s) 354, 510
XspI CTAG 2 cut(s) 888, 1073
Zsp2I ATGCAT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.