FvH4_1g12811

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000703
Physical Location & Seq
Forward (+)
7086658 .. 7087299
642 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g12811.t1

Sequence Viewer

Length: 534 bp
ATGGTTGGAGTTCGTCGTTCCAGAAGAGACCTGCAAGAAGCTCGGGCTGAGGCCGAGCGTCTGAACCAAGAGATGGATGAACGCAGCGCTGCTGCTGCCGCCGCTGTTCCTGTTACCTTATCCCGACTGGAACTTGCGATTGGTGTGCTCACCGATCTCGGTGTGGAATCTCAAGAGATGCTTTCCAAGCAATTTGTTTCACCGGTTGCGTCTCCTGCCCCTGCCCCTGCCCCGGTGCACTCCTCTTCGTCATCCTCTACTTTCTCTAGCTCTGACAACGCCCAGCCATCTCCACCTCCTCTTCCTCTGGCTGGCAGCTCACTCTGGCATGCCATTGCCTTAGACCGGGAGGACCAAGCTGCAAGGGAAACACAAGCTTCTTTGGCTGCATGGCGAGCTCATCATGACCATCTCCGTTCTCAAGCGGTTCTTCCCCATGCCCAAGGAGATGTGCCTCAGGTTCCTGTACCCGCGAATGATGATCTTGTCGACCGTCGCCGGAGCGGGCGGTTGGCTCTTCGTCGAAGTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

18.93

Weight (kDa)

6.29

Isoelectric Point (pI)

92.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 39
AccB7I CCANNNNNTGG 1 cut(s) 73
AccBSI CCGCTC 1 cut(s) 504
AccI GTMKAC 1 cut(s) 489
AccII CGCG 1 cut(s) 473
AciI CCGC 6 cut(s) 99, 102, 425, 471, 504, 508
AfaI GTAC 1 cut(s) 468
AfeI AGCGCT 1 cut(s) 88
AfiI CCNNNNNNNGG 4 cut(s) 73, 232, 311, 345
AgeI ACCGGT 1 cut(s) 202
AjuI GAANNNNNNNTTGG 2 cut(s) 123, 155
AluBI AGCT 6 cut(s) 41, 270, 318, 359, 377, 398
AluI AGCT 6 cut(s) 41, 270, 318, 359, 377, 398
Alw21I GWGCWC 3 cut(s) 150, 240, 400
Alw26I GTCTC 2 cut(s) 21, 216
Alw44I GTGCAC 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 42
Aor51HI AGCGCT 1 cut(s) 88
AoxI GGCC 1 cut(s) 51
ApaLI GTGCAC 1 cut(s) 236
ApeKI GCWGC 7 cut(s) 84, 89, 92, 95, 315, 359, 386
AsiGI ACCGGT 1 cut(s) 202
AspLEI GCGC 1 cut(s) 89
AspS9I GGNCC 1 cut(s) 352
AsuC2I CCSGG 2 cut(s) 233, 347
AsuHPI GGTGA 2 cut(s) 142, 192
AvaI CYCGRG 1 cut(s) 42
AvaII GGWCC 1 cut(s) 352
AxyI CCTNAGG 1 cut(s) 456
BaeGI GKGCMC 1 cut(s) 240
BanII GRGCYC 1 cut(s) 400
Bbv12I GWGCWC 3 cut(s) 150, 240, 400
BbvCI CCTCAGC 1 cut(s) 48
BbvI GCAGC 7 cut(s) 76, 79, 82, 96, 327, 346, 373
BccI CCATC 3 cut(s) 67, 295, 417
BcgI CGANNNNNNTGC 4 cut(s) 23, 57, 127, 161
BcnI CCSGG 2 cut(s) 233, 347
BcoDI GTCTC 2 cut(s) 21, 216
BfaI CTAG 2 cut(s) 267, 532
BfoI RGCGCY 1 cut(s) 90
BfuAI ACCTGC 1 cut(s) 39
BisI GCNGC 9 cut(s) 85, 90, 93, 96, 99, 102, 316, 360, 387
BlsI GCNGC 9 cut(s) 86, 91, 94, 97, 100, 103, 317, 361, 388
Bme1390I CCNGG 2 cut(s) 233, 347
Bme18I GGWCC 1 cut(s) 352
BmeT110I CYCGRG 1 cut(s) 42
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 1 cut(s) 462
BmrFI CCNGG 2 cut(s) 233, 347
BmsI GCATC 1 cut(s) 168
Bpu10I CCTNAGC 1 cut(s) 48
BpuEI CTTGAG 2 cut(s) 156, 405
BpuMI CCSGG 2 cut(s) 233, 347
BsaI GGTCTC 1 cut(s) 21
BsaJI CCNNGG 2 cut(s) 231, 442
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 232, 311, 345
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 132
Bse21I CCTNAGG 1 cut(s) 456
Bse3DI GCAATG 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 231, 442
BseGI GGATG 2 cut(s) 82, 251
BseLI CCNNNNNNNGG 4 cut(s) 73, 232, 311, 345
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 2 cut(s) 39, 470
BseNI ACTGG 1 cut(s) 132
BseRI GAGGAG 2 cut(s) 232, 288
BseSI GKGCMC 1 cut(s) 240
BseXI GCAGC 7 cut(s) 76, 79, 82, 96, 327, 346, 373
BseYI CCCAGC 1 cut(s) 282
Bsh1236I CGCG 1 cut(s) 473
Bsh1285I CGRYCG 1 cut(s) 493
BshFI GGCC 1 cut(s) 53
BshTI ACCGGT 1 cut(s) 202
BsiEI CGRYCG 1 cut(s) 493
BsiHKAI GWGCWC 3 cut(s) 150, 240, 400
BsiHKCI CYCGRG 1 cut(s) 42
BsiSI CCGG 4 cut(s) 203, 233, 346, 499
BslI CCNNNNNNNGG 4 cut(s) 73, 232, 311, 345
BsmAI GTCTC 2 cut(s) 21, 216
BsmBI CGTCTC 1 cut(s) 216
BsnI GGCC 1 cut(s) 53
Bso31I GGTCTC 1 cut(s) 21
BsoBI CYCGRG 1 cut(s) 42
Bsp1286I GDGCHC 3 cut(s) 150, 240, 400
Bsp143I GATC 2 cut(s) 154, 481
BspACI CCGC 6 cut(s) 99, 102, 425, 471, 504, 508
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 2 cut(s) 40, 469
BspFNI CGCG 1 cut(s) 473
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 1 cut(s) 462
BspMI ACCTGC 1 cut(s) 39
BspQI GCTCTTC 1 cut(s) 522
BspTNI GGTCTC 1 cut(s) 21
BsrBI CCGCTC 1 cut(s) 504
BsrDI GCAATG 1 cut(s) 333
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 1 cut(s) 132
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 231, 442
BssMI GATC 2 cut(s) 154, 481
BssT1I CCWWGG 1 cut(s) 442
Bst4CI ACNGT 1 cut(s) 494
Bst6I CTCTTC 4 cut(s) 19, 250, 306, 522
BstC8I GCNNGC 4 cut(s) 313, 330, 396, 506
BstDEI CTNAG 3 cut(s) 48, 340, 456
BstF5I GGATG 2 cut(s) 82, 251
BstFNI CGCG 1 cut(s) 473
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 1 cut(s) 89
BstKTI GATC 2 cut(s) 157, 484
BstMAI GTCTC 2 cut(s) 21, 216
BstMBI GATC 2 cut(s) 154, 481
BstMCI CGRYCG 1 cut(s) 493
BstMWI GCNNNNNNNGC 7 cut(s) 95, 98, 101, 187, 215, 383, 395
BstNSI RCATGY 1 cut(s) 332
BstSCI CCNGG 2 cut(s) 231, 345
BstSLI GKGCMC 1 cut(s) 240
BstUI CGCG 1 cut(s) 473
BstV1I GCAGC 7 cut(s) 76, 79, 82, 96, 327, 346, 373
Bsu36I CCTNAGG 1 cut(s) 456
BsuRI GGCC 1 cut(s) 53
BtsCI GGATG 2 cut(s) 82, 251
BveI ACCTGC 1 cut(s) 39
Cac8I GCNNGC 4 cut(s) 313, 330, 396, 506
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 89
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 352
CseI GACGC 2 cut(s) 47, 198
Csp6I GTAC 1 cut(s) 467
CspAI ACCGGT 1 cut(s) 202
CviAII CATG 4 cut(s) 329, 390, 404, 437
CviQI GTAC 1 cut(s) 467
DdeI CTNAG 3 cut(s) 48, 340, 456
DpnI GATC 2 cut(s) 156, 483
DpnII GATC 2 cut(s) 154, 481
Eam1104I CTCTTC 4 cut(s) 19, 250, 306, 522
EarI CTCTTC 4 cut(s) 19, 250, 306, 522
Ecl136II GAGCTC 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 400
Eco31I GGTCTC 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 352
Eco47III AGCGCT 1 cut(s) 88
Eco53kI GAGCTC 1 cut(s) 398
Eco81I CCTNAGG 1 cut(s) 456
Eco88I CYCGRG 1 cut(s) 42
EcoICRI GAGCTC 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 442
Esp3I CGTCTC 1 cut(s) 216
FaeI CATG 4 cut(s) 332, 393, 407, 440
FaiI YATR 4 cut(s) 330, 391, 405, 438
FalI AAGNNNNNCTT 4 cut(s) 165, 197, 414, 446
FatI CATG 4 cut(s) 328, 389, 403, 436
FauI CCCGC 2 cut(s) 478, 497
FblI GTMKAC 1 cut(s) 489
Fnu4HI GCNGC 9 cut(s) 85, 90, 93, 96, 99, 102, 316, 360, 387
FokI GGATG 2 cut(s) 89, 238
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 9 cut(s) 85, 90, 93, 96, 99, 102, 316, 360, 387
FspBI CTAG 2 cut(s) 267, 532
GlaI GCGC 1 cut(s) 88
GluI GCNGC 9 cut(s) 85, 90, 93, 96, 99, 102, 316, 360, 387
GsaI CCCAGC 1 cut(s) 286
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 1 cut(s) 53
HapII CCGG 4 cut(s) 203, 233, 346, 499
HgaI GACGC 2 cut(s) 47, 198
HhaI GCGC 1 cut(s) 89
Hin1II CATG 4 cut(s) 332, 393, 407, 440
Hin6I GCGC 1 cut(s) 87
HinP1I GCGC 1 cut(s) 87
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HindIII AAGCTT 1 cut(s) 375
HinfI GANTC 1 cut(s) 167
HpaII CCGG 4 cut(s) 203, 233, 346, 499
HphI GGTGA 2 cut(s) 142, 192
Hpy166II GTNNAC 2 cut(s) 238, 490
Hpy188I TCNGA 2 cut(s) 63, 274
Hpy188III TCNNGA 4 cut(s) 21, 123, 173, 404
Hpy8I GTNNAC 2 cut(s) 238, 490
Hpy99I CGWCG 3 cut(s) 18, 498, 525
HpyCH4III ACNGT 1 cut(s) 494
HpyCH4V TGCA 4 cut(s) 34, 238, 362, 389
HpyF10VI GCNNNNNNNGC 7 cut(s) 95, 98, 101, 187, 215, 383, 395
HpyF3I CTNAG 3 cut(s) 48, 340, 456
Hsp92II CATG 4 cut(s) 332, 393, 407, 440
HspAI GCGC 1 cut(s) 87
Kzo9I GATC 2 cut(s) 154, 481
LguI GCTCTTC 1 cut(s) 522
LmnI GCTCC 1 cut(s) 501
Lsp1109I GCAGC 7 cut(s) 76, 79, 82, 96, 327, 346, 373
LweI GCATC 1 cut(s) 168
MaeI CTAG 2 cut(s) 267, 532
MaeIII GTNAC 1 cut(s) 112
MalI GATC 2 cut(s) 156, 483
MbiI CCGCTC 1 cut(s) 504
MboI GATC 2 cut(s) 154, 481
MboII GAAGA 5 cut(s) 36, 237, 293, 422, 509
MhlI GDGCHC 3 cut(s) 150, 240, 400
MluCI AATT 1 cut(s) 191
MnlI CCTC 8 cut(s) 43, 253, 265, 306, 309, 315, 343, 465
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 4 cut(s) 203, 233, 346, 499
MspR9I CCNGG 2 cut(s) 233, 347
MvnI CGCG 1 cut(s) 473
MwoI GCNNNNNNNGC 7 cut(s) 95, 98, 101, 187, 215, 383, 395
NciI CCSGG 2 cut(s) 233, 347
NdeII GATC 2 cut(s) 154, 481
NlaIII CATG 4 cut(s) 332, 393, 407, 440
NlaIV GGNNCC 1 cut(s) 462
NmeAIII GCCGAG 1 cut(s) 79
NspI RCATGY 1 cut(s) 332
PaeI GCATGC 1 cut(s) 332
PagI TCATGA 1 cut(s) 403
PciSI GCTCTTC 1 cut(s) 522
PfeI GAWTC 1 cut(s) 167
PflMI CCANNNNNTGG 1 cut(s) 73
PinAI ACCGGT 1 cut(s) 202
PkrI GCNGC 9 cut(s) 86, 91, 94, 97, 100, 103, 317, 361, 388
Psp124BI GAGCTC 1 cut(s) 400
PspFI CCCAGC 1 cut(s) 282
PspN4I GGNNCC 1 cut(s) 462
PspPI GGNCC 1 cut(s) 352
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
SacI GAGCTC 1 cut(s) 400
SalI GTCGAC 1 cut(s) 488
SapI GCTCTTC 1 cut(s) 522
SatI GCNGC 9 cut(s) 85, 90, 93, 96, 99, 102, 316, 360, 387
Sau3AI GATC 2 cut(s) 154, 481
Sau96I GGNCC 1 cut(s) 352
ScrFI CCNGG 2 cut(s) 233, 347
SduI GDGCHC 3 cut(s) 150, 240, 400
SfaNI GCATC 1 cut(s) 168
SinI GGWCC 1 cut(s) 352
SmlI CTYRAG 2 cut(s) 171, 420
SmoI CTYRAG 2 cut(s) 171, 420
SphI GCATGC 1 cut(s) 332
Sse9I AATT 1 cut(s) 191
SsiI CCGC 6 cut(s) 99, 102, 425, 471, 504, 508
SspMI CTAG 2 cut(s) 267, 532
SstI GAGCTC 1 cut(s) 400
StyD4I CCNGG 2 cut(s) 231, 345
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 1 cut(s) 494
TaqI TCGA 2 cut(s) 489, 523
TasI AATT 1 cut(s) 191
TauI GCSGC 2 cut(s) 101, 104
TfiI GAWTC 1 cut(s) 167
TseI GCWGC 7 cut(s) 84, 89, 92, 95, 315, 359, 386
TspDTI ATGAA 1 cut(s) 93
TspGWI ACGGA 1 cut(s) 404
Van91I CCANNNNNTGG 1 cut(s) 73
VneI GTGCAC 1 cut(s) 236
VpaK11BI GGWCC 1 cut(s) 352
XceI RCATGY 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 489
XspI CTAG 2 cut(s) 267, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.