pycom14g05470

GAG-pre-integrase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000703
Physical Location & Seq
Forward (+)
5315819 .. 5316412
594 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g05470.1

Sequence Viewer

Length: 318 bp
ATGATCGAGTTAGCATCGTGCAAAGTTCGAGGGAAAGAAAGATCGAGCAGCAAAGCGCGAAGACAAATCTTTATGTATTCTTTCTTTCTTAGTGCTTCCTGGCTTGGTAGCCCGAGGATTTTCCCAGTGGTGGGTTTTGCCTCGTTAAATCCTACATCATGTGTGTGCTTTTTATATTCATTTAGTACCCATCCTAGGACTTTCAATTGGCACCAGAGCAGGAATCGGTCTATGGGTTCAGTTTCTCATCCGCCACATTTTGATGGCGACAACTACGCTGCATGGAAGGCAAAGATGAAATCATTTCTCTGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
gag_pre-integrs

Protein Analysis

106

Amino Acids

12.06

Weight (kDa)

10.3

Isoelectric Point (pI)

62.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 210
AccII CGCG 1 cut(s) 58
AciI CCGC 1 cut(s) 251
AfaI GTAC 1 cut(s) 187
AfiI CCNNNNNNNGG 3 cut(s) 130, 131, 195
AgsI TTSAA 1 cut(s) 205
AjnI CCWGG 1 cut(s) 98
Ama87I CYCGRG 1 cut(s) 112
ApeKI GCWGC 2 cut(s) 48, 278
AspA2I CCTAGG 1 cut(s) 194
AspLEI GCGC 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 112
AvrII CCTAGG 1 cut(s) 194
BanI GGYRCC 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 67
BbvI GCAGC 2 cut(s) 60, 265
BccI CCATC 2 cut(s) 198, 257
BciT130I CCWGG 1 cut(s) 100
BfaI CTAG 1 cut(s) 195
BisI GCNGC 2 cut(s) 49, 279
BlnI CCTAGG 1 cut(s) 194
BlsI GCNGC 2 cut(s) 50, 280
Bme1390I CCNGG 1 cut(s) 100
BmeT110I CYCGRG 1 cut(s) 112
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 1 cut(s) 100
BmrI ACTGGG 1 cut(s) 119
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 113, 194
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 131, 195
Bse1I ACTGG 1 cut(s) 125
BseBI CCWGG 1 cut(s) 100
BseDI CCNNGG 2 cut(s) 113, 194
BseGI GGATG 2 cut(s) 190, 247
BseLI CCNNNNNNNGG 3 cut(s) 130, 131, 195
BseNI ACTGG 1 cut(s) 125
BseXI GCAGC 2 cut(s) 60, 265
Bsh1236I CGCG 1 cut(s) 58
BshNI GGYRCC 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 112
BslI CCNNNNNNNGG 3 cut(s) 130, 131, 195
BsoBI CYCGRG 1 cut(s) 112
Bsp143I GATC 2 cut(s) 3, 41
BspACI CCGC 1 cut(s) 251
BspFNI CGCG 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 210
BsrI ACTGG 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 113, 194
BssMI GATC 2 cut(s) 3, 41
BssT1I CCWWGG 1 cut(s) 194
Bst2UI CCWGG 1 cut(s) 100
BstDEI CTNAG 1 cut(s) 89
BstF5I GGATG 2 cut(s) 190, 247
BstFNI CGCG 1 cut(s) 58
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 2 cut(s) 6, 44
BstMBI GATC 2 cut(s) 3, 41
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstNI CCWGG 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 98
BstUI CGCG 1 cut(s) 58
BstV1I GCAGC 2 cut(s) 60, 265
BstV2I GAAGAC 1 cut(s) 67
BtsCI GGATG 2 cut(s) 190, 247
BtsIMutI CAGTG 1 cut(s) 132
CfoI GCGC 1 cut(s) 58
Csp6I GTAC 1 cut(s) 186
CviAII CATG 2 cut(s) 159, 282
CviJI RGCY 3 cut(s) 103, 111, 314
CviKI_1 RGCY 3 cut(s) 103, 111, 314
CviQI GTAC 1 cut(s) 186
DdeI CTNAG 1 cut(s) 89
DpnI GATC 2 cut(s) 5, 43
DpnII GATC 2 cut(s) 3, 41
EciI GGCGGA 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 194
Eco88I CYCGRG 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 2 cut(s) 162, 285
FaiI YATR 5 cut(s) 74, 160, 175, 233, 283
FatI CATG 2 cut(s) 158, 281
Fnu4HI GCNGC 2 cut(s) 49, 279
FokI GGATG 2 cut(s) 177, 234
Fsp4HI GCNGC 2 cut(s) 49, 279
FspBI CTAG 1 cut(s) 195
GlaI GCGC 1 cut(s) 57
GluI GCNGC 2 cut(s) 49, 279
HhaI GCGC 1 cut(s) 58
Hin1II CATG 2 cut(s) 162, 285
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HinfI GANTC 1 cut(s) 223
HpyAV CCTTC 1 cut(s) 280
HpyCH4V TGCA 2 cut(s) 21, 281
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpyF3I CTNAG 1 cut(s) 89
Hsp92II CATG 2 cut(s) 162, 285
HspAI GCGC 1 cut(s) 56
Kzo9I GATC 2 cut(s) 3, 41
LpnPI CCDG 6 cut(s) 85, 112, 138, 205, 227, 295
Lsp1109I GCAGC 2 cut(s) 60, 265
LweI GCATC 1 cut(s) 23
MaeI CTAG 1 cut(s) 195
MalI GATC 2 cut(s) 5, 43
MboI GATC 2 cut(s) 3, 41
MboII GAAGA 1 cut(s) 72
MfeI CAATTG 1 cut(s) 205
MluCI AATT 1 cut(s) 205
MnlI CCTC 3 cut(s) 23, 108, 151
MseI TTAA 1 cut(s) 146
MslI CAYNNNNRTG 2 cut(s) 163, 261
MspR9I CCNGG 1 cut(s) 100
MunI CAATTG 1 cut(s) 205
MvaI CCWGG 1 cut(s) 100
MvnI CGCG 1 cut(s) 58
MwoI GCNNNNNNNGC 1 cut(s) 287
NdeII GATC 2 cut(s) 3, 41
NlaIII CATG 2 cut(s) 162, 285
NlaIV GGNNCC 1 cut(s) 212
PfeI GAWTC 1 cut(s) 223
PkrI GCNGC 2 cut(s) 50, 280
Psp6I CCWGG 1 cut(s) 98
PspGI CCWGG 1 cut(s) 98
PspN4I GGNNCC 1 cut(s) 212
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
RseI CAYNNNNRTG 2 cut(s) 163, 261
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 2 cut(s) 49, 279
Sau3AI GATC 2 cut(s) 3, 41
ScrFI CCNGG 1 cut(s) 100
SfaNI GCATC 1 cut(s) 23
SmiMI CAYNNNNRTG 2 cut(s) 163, 261
Sse9I AATT 1 cut(s) 205
SsiI CCGC 1 cut(s) 251
SspMI CTAG 1 cut(s) 195
StyD4I CCNGG 1 cut(s) 98
StyI CCWWGG 1 cut(s) 194
TaqI TCGA 3 cut(s) 6, 28, 44
TaqII GACCGA 1 cut(s) 216
TasI AATT 1 cut(s) 205
TfiI GAWTC 1 cut(s) 223
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TscAI CASTG 1 cut(s) 132
TseI GCWGC 2 cut(s) 48, 278
TspDTI ATGAA 2 cut(s) 168, 311
TspRI CASTG 1 cut(s) 132
XmaJI CCTAGG 1 cut(s) 194
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.