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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 147, 297 |
| AcoI | YGGCCR | 1 cut(s) | 162 |
| AcsI | RAATTY | 1 cut(s) | 137 |
| AfaI | GTAC | 1 cut(s) | 339 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 14 |
| AgsI | TTSAA | 2 cut(s) | 239, 410 |
| AluBI | AGCT | 1 cut(s) | 73 |
| AluI | AGCT | 1 cut(s) | 73 |
| Alw21I | GWGCWC | 2 cut(s) | 225, 395 |
| Alw44I | GTGCAC | 2 cut(s) | 221, 391 |
| AoxI | GGCC | 1 cut(s) | 162 |
| ApaLI | GTGCAC | 2 cut(s) | 221, 391 |
| ApoI | RAATTY | 1 cut(s) | 137 |
| AseI | ATTAAT | 1 cut(s) | 350 |
| AspLEI | GCGC | 1 cut(s) | 361 |
| AsuHPI | GGTGA | 5 cut(s) | 35, 281, 296, 311, 467 |
| BaeGI | GKGCMC | 2 cut(s) | 225, 395 |
| Bbv12I | GWGCWC | 2 cut(s) | 225, 395 |
| BccI | CCATC | 4 cut(s) | 260, 287, 311, 458 |
| BfaI | CTAG | 1 cut(s) | 87 |
| BisI | GCNGC | 1 cut(s) | 147 |
| BlsI | GCNGC | 1 cut(s) | 148 |
| BmsI | GCATC | 1 cut(s) | 140 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 14 |
| Bse3DI | GCAATG | 1 cut(s) | 509 |
| BseGI | GGATG | 3 cut(s) | 280, 436, 451 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 14 |
| BseMI | GCAATG | 1 cut(s) | 509 |
| BseMII | CTCAG | 1 cut(s) | 23 |
| BseRI | GAGGAG | 1 cut(s) | 168 |
| BseSI | GKGCMC | 2 cut(s) | 225, 395 |
| BshFI | GGCC | 1 cut(s) | 164 |
| BsiHKAI | GWGCWC | 2 cut(s) | 225, 395 |
| BsiSI | CCGG | 1 cut(s) | 165 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 14 |
| BsmI | GAATGC | 2 cut(s) | 191, 361 |
| BsnI | GGCC | 1 cut(s) | 164 |
| Bsp1286I | GDGCHC | 2 cut(s) | 225, 395 |
| Bsp143I | GATC | 1 cut(s) | 487 |
| BspACI | CCGC | 2 cut(s) | 147, 297 |
| BspANI | GGCC | 1 cut(s) | 164 |
| BspCNI | CTCAG | 1 cut(s) | 22 |
| BsrDI | GCAATG | 1 cut(s) | 509 |
| BssMI | GATC | 1 cut(s) | 487 |
| Bst4CI | ACNGT | 3 cut(s) | 23, 29, 61 |
| Bst6I | CTCTTC | 3 cut(s) | 39, 90, 99 |
| BstDEI | CTNAG | 1 cut(s) | 9 |
| BstEII | GGTNACC | 1 cut(s) | 23 |
| BstF5I | GGATG | 3 cut(s) | 280, 436, 451 |
| BstHHI | GCGC | 1 cut(s) | 361 |
| BstKTI | GATC | 1 cut(s) | 490 |
| BstMBI | GATC | 1 cut(s) | 487 |
| BstPI | GGTNACC | 1 cut(s) | 23 |
| BstSLI | GKGCMC | 2 cut(s) | 225, 395 |
| BsuRI | GGCC | 1 cut(s) | 164 |
| BtsCI | GGATG | 3 cut(s) | 280, 436, 451 |
| CfoI | GCGC | 1 cut(s) | 361 |
| Csp6I | GTAC | 1 cut(s) | 338 |
| CviJI | RGCY | 5 cut(s) | 18, 73, 164, 197, 367 |
| CviKI_1 | RGCY | 5 cut(s) | 18, 73, 164, 197, 367 |
| CviQI | GTAC | 1 cut(s) | 338 |
| DdeI | CTNAG | 1 cut(s) | 9 |
| DpnI | GATC | 1 cut(s) | 489 |
| DpnII | GATC | 1 cut(s) | 487 |
| EaeI | YGGCCR | 1 cut(s) | 162 |
| Eam1104I | CTCTTC | 3 cut(s) | 39, 90, 99 |
| EarI | CTCTTC | 3 cut(s) | 39, 90, 99 |
| Eco91I | GGTNACC | 1 cut(s) | 23 |
| EcoO65I | GGTNACC | 1 cut(s) | 23 |
| EcoT22I | ATGCAT | 1 cut(s) | 133 |
| Fnu4HI | GCNGC | 1 cut(s) | 147 |
| FokI | GGATG | 3 cut(s) | 287, 443, 458 |
| Fsp4HI | GCNGC | 1 cut(s) | 147 |
| FspBI | CTAG | 1 cut(s) | 87 |
| GlaI | GCGC | 1 cut(s) | 360 |
| GluI | GCNGC | 1 cut(s) | 147 |
| HaeIII | GGCC | 1 cut(s) | 164 |
| HapII | CCGG | 1 cut(s) | 165 |
| HhaI | GCGC | 1 cut(s) | 361 |
| Hin6I | GCGC | 1 cut(s) | 359 |
| HinP1I | GCGC | 1 cut(s) | 359 |
| HinfI | GANTC | 1 cut(s) | 321 |
| HpaII | CCGG | 1 cut(s) | 165 |
| HphI | GGTGA | 5 cut(s) | 35, 281, 296, 311, 467 |
| Hpy166II | GTNNAC | 2 cut(s) | 223, 393 |
| Hpy188I | TCNGA | 1 cut(s) | 492 |
| Hpy8I | GTNNAC | 2 cut(s) | 223, 393 |
| Hpy99I | CGWCG | 1 cut(s) | 146 |
| HpyAV | CCTTC | 2 cut(s) | 63, 94 |
| HpyCH4III | ACNGT | 3 cut(s) | 23, 29, 61 |
| HpyCH4V | TGCA | 4 cut(s) | 131, 189, 223, 393 |
| HpyF3I | CTNAG | 1 cut(s) | 9 |
| HspAI | GCGC | 1 cut(s) | 359 |
| Kzo9I | GATC | 1 cut(s) | 487 |
| LpnPI | CCDG | 2 cut(s) | 135, 178 |
| LweI | GCATC | 1 cut(s) | 140 |
| MaeI | CTAG | 1 cut(s) | 87 |
| MaeIII | GTNAC | 3 cut(s) | 23, 242, 413 |
| MalI | GATC | 1 cut(s) | 489 |
| MboI | GATC | 1 cut(s) | 487 |
| MhlI | GDGCHC | 2 cut(s) | 225, 395 |
| MluCI | AATT | 3 cut(s) | 137, 174, 305 |
| MmeI | TCCRAC | 1 cut(s) | 140 |
| MnlI | CCTC | 8 cut(s) | 18, 42, 146, 265, 271, 328, 335, 442 |
| Mph1103I | ATGCAT | 1 cut(s) | 133 |
| MseI | TTAA | 4 cut(s) | 69, 207, 350, 517 |
| MspI | CCGG | 1 cut(s) | 165 |
| Mva1269I | GAATGC | 2 cut(s) | 191, 361 |
| NdeII | GATC | 1 cut(s) | 487 |
| NmuCI | GTSAC | 3 cut(s) | 23, 242, 413 |
| NsiI | ATGCAT | 1 cut(s) | 133 |
| PctI | GAATGC | 2 cut(s) | 191, 361 |
| PfeI | GAWTC | 1 cut(s) | 321 |
| PkrI | GCNGC | 1 cut(s) | 148 |
| PshBI | ATTAAT | 1 cut(s) | 350 |
| PspEI | GGTNACC | 1 cut(s) | 23 |
| RsaI | GTAC | 1 cut(s) | 339 |
| RsaNI | GTAC | 1 cut(s) | 338 |
| SaqAI | TTAA | 4 cut(s) | 69, 207, 350, 517 |
| SatI | GCNGC | 1 cut(s) | 147 |
| Sau3AI | GATC | 1 cut(s) | 487 |
| SduI | GDGCHC | 2 cut(s) | 225, 395 |
| SetI | ASST | 7 cut(s) | 10, 55, 75, 88, 127, 339, 403 |
| SfaNI | GCATC | 1 cut(s) | 140 |
| SgeI | CNNG | 6 cut(s) | 99, 162, 177, 337, 339, 353 |
| Sse9I | AATT | 3 cut(s) | 137, 174, 305 |
| SsiI | CCGC | 2 cut(s) | 147, 297 |
| SspMI | CTAG | 1 cut(s) | 87 |
| TaaI | ACNGT | 3 cut(s) | 23, 29, 61 |
| TasI | AATT | 3 cut(s) | 137, 174, 305 |
| TauI | GCSGC | 1 cut(s) | 149 |
| TfiI | GAWTC | 1 cut(s) | 321 |
| Tru1I | TTAA | 4 cut(s) | 69, 207, 350, 517 |
| Tru9I | TTAA | 4 cut(s) | 69, 207, 350, 517 |
| TseFI | GTSAC | 3 cut(s) | 23, 242, 413 |
| Tsp45I | GTSAC | 3 cut(s) | 23, 242, 413 |
| TspDTI | ATGAA | 8 cut(s) | 17, 53, 108, 198, 264, 368, 447, 492 |
| VneI | GTGCAC | 2 cut(s) | 221, 391 |
| VspI | ATTAAT | 1 cut(s) | 350 |
| XapI | RAATTY | 1 cut(s) | 137 |
| XspI | CTAG | 1 cut(s) | 87 |
| Zsp2I | ATGCAT | 1 cut(s) | 133 |