FvH4_4g14651

spliceosomal complex assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000703
Physical Location & Seq
Reverse (-)
18352503 .. 18359602
7100 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g14651.t1

Sequence Viewer

Length: 3774 bp
ATGCTCAAAGGAATGGATTTTGCTCAATGGAAACTTCAAATGAGATCATTCATTTATGGCATTGATTTTCATGCATGGAGAAGTGTGGAACGTGGCTGGAAAGCTCCTGAGATTTCTACGGATGGAAAAGCTCCAGAAGGGTCGTCATCTAAATTAAAGGACGAAGATGATTGGGATGAAAAAGAGATCAAACTGAGTGAAGCTAATCACAAAGCCTTAAATGCTCTCTATGCTGCTCTAAGCAGAGATGAAAAAAAGAAGGTTCAGAATTGTGTTACCGCCAAACAGGTTTGGGATAAATTATGTGTTCTTCATGAAGGTAATGATGTTGTAAAAGAACAAAGGTTACAACAATACTATTCTGAAATTGATGCTTTGAAAATGAAAGATGATGAATCTATTGATGATTTTTATGCTCGTTTTACTGATCTTTCTAGTTTATGTGAAAGCTTGGGACGTCCTTTAAAAGAATCTGATATTGTCAGGAAAATTTTGAGATCTCTTCCAAAGAAGTACAGGATAAAAAAGACGACGATTCAGGAGCAATACAACTTGAATGAATATTCAGTAGATTTGTTGATCGGAAATCTGAAGGCTTGGGAAATGGAACTTGAAGATGACGAAAAACCTAAAGGTACAAGTTCAAAAAATATTGCTTTGAATGTCTCTAAGAAGTCTGAAGAGTCTGAGCATAATGAGTTGTTACTTTTGACTAAAGAATTTAAACGCATTCTGAATCAAAGAAATTCAAAACCTAATGTGCGAAACAATTTCAATTCTAATAAGAGGTTTAATGGAAATAATAAGAATTTCGAAAAACAAGGTGATAATTCGTTTCAAAAGAAAAATGAAACTGGTAATAGTAGTAATGGAAATTATCGGGGTCAAAAGGGTCGTTGTTATGAATGTGGTGGCTATGGCCACATTGCTACTGACTGTGCAAATAGAAAGTCTGATACAGGGAAAAAGGCGTACAGATCCTCATGGAGTGATGAGGAAGAAGATGATTCCTCGAAAGGAAACAAAACTTTTGCTCTTGTGTCTTCTTTTTCTCTTGATTATTCAGATTCAGATGATGAGTTTGAGGAAGATGATGAACTAATGGTACATCATAAGAGGGTTCTAAAGGCTGCAGAACTGGTATCTGAAAAAAATGAGGCTTTGAAGTTTCAAGTAGGAATGCTACAAGAAGAAAAACAAAAATGGGAACTTGAGAAACTTGAGTTAATGAGAAAATGTGAGGAGGATCATAACATGAGTAGTGAAGATTGGAAAGTGGAAAGAAAACGCCTTCTTGCAAAGATTCAGATACTCCAAGAAGAGGTAAATGAAAAAGATAAATTTATTTTGCAACTAACTTATAAAGTTGATAAATTACAGGAATCTCTTGAAAAGGCGGAAGACAAGTTCGGCCAGTTCAAAATGAGCTCTCAATCTCTGTCAGAAATCCTAAGTTCGGGAAAATCTTTTAATGATAGATCTGGTATTGGCTATTCTGGGAAACAAACCCGAGCTAGTCATAAAGTGGTGAATGCTTCATCATCAACTCAACCTCTTGAGTTGCTTCACATGGATTTGATGGGACCTTCAGCGACATCAAGTATGGCAGGTAAAGAATACATTTTTGTTGTTGTTGATGATCATTCTCGTTTTGCCTGGGTTGATTTCTTAAAAGATAAAAGTGAGACATTTAGTTCTTTTTCCACTTTGAGTAAACGTCTTTTAGTTGAAAAACAACAAGAAAATTATCAAATTGCCAAGATTAGGTCAGATAATGGAACTGAGTTTAAGAATTCTAAATTTGCTAAATATTGTCGAGATCACGGTGTGTTTCATGAGTTTTCAGCTCCTATAACTCCACAGCAAAATGGAATTGTAGAACGTAAGAACAGATCTCTCTTAGACATGGCCAGGGTCATGCTTCATTCGGCCGGACTTAGTGAAAACTTCTGGGCTGAGGCTATTAACACAGCTTGTTACGTAGGAAATCGTGTTCTTCTTCGATCAGGAACAAAGTCCACTCCTTATGAACTATGGAAAGGTAAAAAGCCGAATGTAAGTCATTTCCATGTGTTTGGTAACCCTTGCTATGTTTTAAAAGACCGGGAGCGTCTTGCCAAGTTTGCACCTAGAGCAGATGAAGGTATTTTTCTTGGTTATTCTCTTGATAGTCGTGCTTACAGAGTTTACAACAAAGAAACTAAAACAGTTATGGATTCAATTAATGTTACAGTTGATGATAATGCAGATTTGTCTGTTGTTAATGATTCAGATGTGTTTAAATCAAATGACAATAGTAGTTCTGTGCAGGAAAAGACTAATGAAGTTGATAAAAATTTACAACCTGCTACAAAATATAGAACTGGTTTTAAGCAGGTCCGTAAAGATCACTCAACAGCTGATGTTATTGGTGATATTGGTGATGGTATGCGGACCAGGGGGAGAATGGTAGATTATCCTTCTAAAAATGAAGATAATTCTGAGAGCTCTACTGCAGAATTTGTTGAAATAAACAAAGCCTTGGTATGTTTTATTAAGGAAAAAATTAAGGAGACTAACAAACTAACTTGTTTTGGTTTTGTGTCTCTGATTGAACCCAAAAATATCAAAGAAGCTCTTATGGATGATGACTGGATCAATGCAATGCAAGAAGAGTTAAATCAATTTGCAAAAAATGAGGTATGGATCTTGGTTCCTAGACCAGAAAACACTAATGTTATTGGTACTAAATGGATTTTCAGGAATAAGATGGATGAGCATGGTACTATCACTCGGAATAAAGCTAGATTGGTGGCTCAAGGTTATTCACAGGTTGAGGGATTGGATTTTGATGAAACCTTTGCTCCTGTGGCTCGGTTAGAATCTATAAGACTTCTATTAGCTATTGCGTGTCACCGTCAGTTCAAACTTTTTCAAATGGATGTTAAAACTGCATTTCTAAATGGGGATGTTCACGAAGAGGTGTTTGTGGAGCAACCACCTGGCTTTAAAGATATTCACCATCCTGAGTATGTTTGGAAACTAAAAAAGGCTGTCTACGGTCTGAAGCAAGCTCCTCGTGCCTGGTATGATATCTTGATTCACCAAGATTTTGATCTTCTGCCGAGATCATCTTTTAGAGTTTTTGTCGCCATGGTTGGAGTTCGTCGTTCCAGAAGAGACCTGCAAGAAGCTCGGGCTGAGGCCGAGCGTCTGAACCAAGAGATGGATGAACGCAGCGCTGCTGCTGCCGCCGCTGTTCCTGTTACCGTCGGCGATCTCACACAAGCCTTCTCCCGGCTTGGTGTTCATCTGCAATCCCAGGTTGATCGAATCCACCAAGTTCATCTCTTTCATCATGAACGTATTACTGCTCAGTTATCCCGACTGGAACTTGCGATTGGTGTGCTCACCGATCTCGGTGTGGAATCTCAAGAGATGCTTTCCAAGCAATTTGTTTCACCGGTTGCGTCTCCTGCCCCTGCCCCTGCCCCGGTGCACTCCTCTTCGTCATCCTCTACTTTCTCTAGCTCTGACAACGCCCAGCCATCTCCACCTCCTCTTCCTCTGGCTGGCAGCTCACTCTGGCATGCCATTGCCTTAGACCGGGAGGACCAAGCTGCAAGGGAAACACAAGCTTCTTTGGCTGCCTGGCGAGCTCATCATGACCATCTCCGTTCTCAAGCGGTTCTTCCCCATGCCCAAGGAGATGTGCCTCAGGTTCCTGTACCCGCGAATGATGATCTTGTCGACCGTCGCCGGAGCGGGCGGTTGGCTCTTCGTCGAAGTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve zf-CCHC

Protein Analysis

1258

Amino Acids

143.15

Weight (kDa)

6.61

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 25 - 207 2.1e-25 gag-polypeptide of LTR copia-type
rve PF00665 516 - 620 4.1e-12 Integrase core domain
SH3_retrovirus PF25597 695 - 752 3e-16 Retroviral polymerase SH3-like domain
RVT_2 PF07727 892 - 1026 1.7e-49 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1362
AatII GACGTC 1 cut(s) 460
Acc36I ACCTGC 4 cut(s) 1598, 2353, 2365, 3171
AccB7I CCANNNNNTGG 1 cut(s) 3205
AccBSI CCGCTC 1 cut(s) 3744
AccI GTMKAC 2 cut(s) 3036, 3729
AccII CGCG 1 cut(s) 3713
AciI CCGC 9 cut(s) 279, 1397, 2431, 3231, 3234, 3665, 3711, 3744, 3748
AclWI GGATC 4 cut(s) 972, 1254, 2642, 2693
AcoI YGGCCR 4 cut(s) 919, 1411, 1908, 1929
AcsI RAATTY 9 cut(s) 489, 719, 745, 808, 1340, 1792, 1799, 2335, 2498
AcuI CTGAAG 4 cut(s) 611, 699, 1572, 3065
AcyI GRCGYC 1 cut(s) 457
AdeI CACNNNGTG 1 cut(s) 1828
AfaI GTAC 7 cut(s) 515, 637, 974, 1107, 2725, 2764, 3708
AfeI AGCGCT 1 cut(s) 3220
AfiI CCNNNNNNNGG 8 cut(s) 1321, 1456, 1764, 3205, 3276, 3472, 3551, 3585
AgeI ACCGGT 1 cut(s) 3442
AjnI CCWGG 7 cut(s) 1655, 1910, 2435, 2980, 3062, 3300, 3629
AjuI GAANNNNNNNTTGG 2 cut(s) 3363, 3395
AloI GAACNNNNNNTCC 4 cut(s) 1391, 1423, 1977, 2009
Alw21I GWGCWC 5 cut(s) 1430, 2489, 3390, 3480, 3640
Alw26I GTCTC 6 cut(s) 670, 1679, 2546, 2589, 3153, 3456
Alw44I GTGCAC 1 cut(s) 3476
AlwI GGATC 4 cut(s) 972, 1254, 2642, 2693
Ama87I CYCGRG 2 cut(s) 1509, 3174
Aor51HI AGCGCT 1 cut(s) 3220
AoxI GGCC 5 cut(s) 919, 1411, 1908, 1929, 3183
ApaLI GTGCAC 1 cut(s) 3476
ApeKI GCWGC 9 cut(s) 233, 1130, 3216, 3221, 3224, 3227, 3555, 3599, 3626
ApoI RAATTY 9 cut(s) 489, 719, 745, 808, 1340, 1792, 1799, 2335, 2498
ArsI GACNNNNNNTTYG 2 cut(s) 935, 967
AseI ATTAAT 1 cut(s) 2223
AsiGI ACCGGT 1 cut(s) 3442
AspLEI GCGC 1 cut(s) 3221
AspS9I GGNCC 4 cut(s) 1583, 2377, 2433, 3592
AsuC2I CCSGG 4 cut(s) 2105, 3277, 3473, 3587
AsuHPI GGTGA 9 cut(s) 836, 1540, 2423, 2432, 2885, 2990, 3074, 3382, 3432
AsuII TTCGAA 1 cut(s) 813
AvaI CYCGRG 2 cut(s) 1509, 3174
AvaII GGWCC 4 cut(s) 1583, 2377, 2433, 3592
AxyI CCTNAGG 1 cut(s) 3696
BaeGI GKGCMC 1 cut(s) 3480
BalI TGGCCA 2 cut(s) 921, 1910
BanII GRGCYC 3 cut(s) 1430, 2489, 3640
BauI CACGAG 1 cut(s) 3057
BbsI GAAGAC 2 cut(s) 1035, 1407
Bbv12I GWGCWC 5 cut(s) 1430, 2489, 3390, 3480, 3640
BbvCI CCTCAGC 2 cut(s) 1956, 3180
BbvI GCAGC 9 cut(s) 220, 1117, 3208, 3211, 3214, 3228, 3567, 3586, 3613
BccI CCATC 8 cut(s) 116, 1573, 2417, 2743, 3009, 3199, 3535, 3657
BcgI CGANNNNNNTGC 6 cut(s) 3038, 3072, 3155, 3189, 3367, 3401
BciT130I CCWGG 7 cut(s) 1657, 1912, 2437, 2982, 3064, 3302, 3631
BclI TGATCA 1 cut(s) 1639
BcnI CCSGG 4 cut(s) 2105, 3277, 3473, 3587
BcoDI GTCTC 6 cut(s) 670, 1679, 2546, 2589, 3153, 3456
BfaI CTAG 7 cut(s) 435, 1515, 2130, 2697, 2784, 3507, 3772
BfmI CTRYAG 2 cut(s) 1131, 2493
BfoI RGCGCY 1 cut(s) 3222
BfuAI ACCTGC 4 cut(s) 1598, 2353, 2365, 3171
BglII AGATCT 3 cut(s) 497, 1478, 1892
Bme18I GGWCC 4 cut(s) 1583, 2377, 2433, 3592
BmeT110I CYCGRG 2 cut(s) 1509, 3174
BmgT120I GGNCC 4 cut(s) 1583, 2377, 2433, 3592
BmiI GGNNCC 3 cut(s) 1584, 2694, 3702
BmsI GCATC 2 cut(s) 361, 3408
BpiI GAAGAC 2 cut(s) 1035, 1407
BpmI CTGGAG 1 cut(s) 117
Bpu10I CCTNAGC 2 cut(s) 1956, 3180
Bpu14I TTCGAA 1 cut(s) 813
BpuEI CTTGAG 6 cut(s) 1232, 1241, 1577, 2781, 3396, 3645
BpuMI CCSGG 4 cut(s) 2105, 3277, 3473, 3587
BsaAI YACGTR 1 cut(s) 1981
BsaBI GATNNNNATC 1 cut(s) 3093
BsaHI GRCGYC 1 cut(s) 457
BsaI GGTCTC 1 cut(s) 3153
BsaJI CCNNGG 8 cut(s) 1656, 1911, 2436, 2520, 3132, 3300, 3471, 3682
BsaWI WCCGGW 1 cut(s) 3442
BsaXI ACNNNNNCTCC 2 cut(s) 2827, 2857
Bsc4I CCNNNNNNNGG 8 cut(s) 1321, 1456, 1764, 3205, 3276, 3472, 3551, 3585
Bse118I RCCGGY 1 cut(s) 3442
Bse1I ACTGG 6 cut(s) 859, 1143, 1414, 2368, 2636, 3372
Bse21I CCTNAGG 1 cut(s) 3696
Bse3DI GCAATG 3 cut(s) 924, 2649, 3573
Bse8I GATNNNNATC 1 cut(s) 3093
BseBI CCWGG 7 cut(s) 1657, 1912, 2437, 2982, 3064, 3302, 3631
BseDI CCNNGG 8 cut(s) 1656, 1911, 2436, 2520, 3132, 3300, 3471, 3682
BseGI GGATG 9 cut(s) 127, 181, 2629, 2758, 2926, 2953, 3001, 3214, 3491
BseJI GATNNNNATC 1 cut(s) 3093
BseLI CCNNNNNNNGG 8 cut(s) 1321, 1456, 1764, 3205, 3276, 3472, 3551, 3585
BseMI GCAATG 3 cut(s) 924, 2649, 3573
BseNI ACTGG 6 cut(s) 859, 1143, 1414, 2368, 2636, 3372
BseRI GAGGAG 4 cut(s) 1256, 3045, 3472, 3528
BseSI GKGCMC 1 cut(s) 3480
BseX3I CGGCCG 1 cut(s) 1929
BseXI GCAGC 9 cut(s) 220, 1117, 3208, 3211, 3214, 3228, 3567, 3586, 3613
BseYI CCCAGC 1 cut(s) 3522
BsgI GTGCAG 1 cut(s) 2327
Bsh1236I CGCG 1 cut(s) 3713
Bsh1285I CGRYCG 2 cut(s) 1932, 3733
BshFI GGCC 5 cut(s) 921, 1413, 1910, 1931, 3185
BshTI ACCGGT 1 cut(s) 3442
BsiEI CGRYCG 2 cut(s) 1932, 3733
BsiHKAI GWGCWC 5 cut(s) 1430, 2489, 3390, 3480, 3640
BsiHKCI CYCGRG 2 cut(s) 1509, 3174
BsiSI CCGG 7 cut(s) 1932, 2104, 3277, 3443, 3473, 3586, 3739
BslFI GGGAC 2 cut(s) 468, 1596
BslI CCNNNNNNNGG 8 cut(s) 1321, 1456, 1764, 3205, 3276, 3472, 3551, 3585
BsmAI GTCTC 6 cut(s) 670, 1679, 2546, 2589, 3153, 3456
BsmBI CGTCTC 1 cut(s) 3456
BsmFI GGGAC 2 cut(s) 468, 1596
BsmI GAATGC 3 cut(s) 729, 1185, 1537
BsnI GGCC 5 cut(s) 921, 1413, 1910, 1931, 3185
Bso31I GGTCTC 1 cut(s) 3153
BsoBI CYCGRG 2 cut(s) 1509, 3174
Bsp119I TTCGAA 1 cut(s) 813
Bsp1286I GDGCHC 5 cut(s) 1430, 2489, 3390, 3480, 3640
Bsp19I CCATGG 1 cut(s) 3132
BspACI CCGC 9 cut(s) 279, 1397, 2431, 3231, 3234, 3665, 3711, 3744, 3748
BspANI GGCC 5 cut(s) 921, 1413, 1910, 1931, 3185
BspFNI CGCG 1 cut(s) 3713
BspHI TCATGA 4 cut(s) 313, 1834, 3337, 3643
BspLI GGNNCC 3 cut(s) 1584, 2694, 3702
BspMAI CTGCAG 2 cut(s) 1135, 2497
BspMI ACCTGC 4 cut(s) 1598, 2353, 2365, 3171
BspPI GGATC 4 cut(s) 972, 1254, 2642, 2693
BspQI GCTCTTC 1 cut(s) 3762
BspT104I TTCGAA 1 cut(s) 813
BspTNI GGTCTC 1 cut(s) 3153
BsrBI CCGCTC 1 cut(s) 3744
BsrDI GCAATG 3 cut(s) 924, 2649, 3573
BsrFI RCCGGY 1 cut(s) 3442
BsrI ACTGG 6 cut(s) 859, 1143, 1414, 2368, 2636, 3372
BssAI RCCGGY 1 cut(s) 3442
BssECI CCNNGG 8 cut(s) 1656, 1911, 2436, 2520, 3132, 3300, 3471, 3682
BssNI GRCGYC 1 cut(s) 457
BssSI CACGAG 1 cut(s) 3057
BssT1I CCWWGG 3 cut(s) 2520, 3132, 3682
Bst2BI CACGAG 1 cut(s) 3057
Bst2UI CCWGG 7 cut(s) 1657, 1912, 2437, 2982, 3064, 3302, 3631
Bst4CI ACNGT 8 cut(s) 938, 1826, 2209, 2233, 2897, 3041, 3250, 3734
Bst6I CTCTTC 9 cut(s) 507, 675, 1314, 2646, 2952, 3151, 3490, 3546, 3762
BstACI GRCGYC 1 cut(s) 457
BstBAI YACGTR 1 cut(s) 1981
BstBI TTCGAA 1 cut(s) 813
BstC8I GCNNGC 5 cut(s) 3051, 3553, 3570, 3636, 3746
BstDSI CCRYGG 1 cut(s) 3132
BstEII GGTNACC 1 cut(s) 2078
BstF5I GGATG 9 cut(s) 127, 181, 2629, 2758, 2926, 2953, 3001, 3214, 3491
BstFNI CGCG 1 cut(s) 3713
BstH2I RGCGCY 1 cut(s) 3222
BstHHI GCGC 1 cut(s) 3221
BstMAI GTCTC 6 cut(s) 670, 1679, 2546, 2589, 3153, 3456
BstMCI CGRYCG 2 cut(s) 1932, 3733
BstNI CCWGG 7 cut(s) 1657, 1912, 2437, 2982, 3064, 3302, 3631
BstNSI RCATGY 1 cut(s) 3572
BstPI GGTNACC 1 cut(s) 2078
BstSFI CTRYAG 2 cut(s) 1131, 2493
BstSLI GKGCMC 1 cut(s) 3480
BstSNI TACGTA 1 cut(s) 1981
BstUI CGCG 1 cut(s) 3713
BstV1I GCAGC 9 cut(s) 220, 1117, 3208, 3211, 3214, 3228, 3567, 3586, 3613
BstV2I GAAGAC 2 cut(s) 1035, 1407
BstX2I RGATCY 5 cut(s) 497, 977, 1478, 1892, 2685
BstXI CCANNNNNNTGG 1 cut(s) 2075
BstYI RGATCY 5 cut(s) 497, 977, 1478, 1892, 2685
BstZI CGGCCG 1 cut(s) 1929
Bsu36I CCTNAGG 1 cut(s) 3696
BsuRI GGCC 5 cut(s) 921, 1413, 1910, 1931, 3185
BtgI CCRYGG 1 cut(s) 3132
BtsCI GGATG 9 cut(s) 127, 181, 2629, 2758, 2926, 2953, 3001, 3214, 3491
BveI ACCTGC 4 cut(s) 1598, 2353, 2365, 3171
Cac8I GCNNGC 5 cut(s) 3051, 3553, 3570, 3636, 3746
CciI TCATGA 4 cut(s) 313, 1834, 3337, 3643
CfoI GCGC 1 cut(s) 3221
Cfr10I RCCGGY 1 cut(s) 3442
Cfr13I GGNCC 4 cut(s) 1583, 2377, 2433, 3592
CseI GACGC 3 cut(s) 2099, 3179, 3438
Csp6I GTAC 7 cut(s) 514, 636, 973, 1106, 2724, 2763, 3707
CspAI ACCGGT 1 cut(s) 3442
CviQI GTAC 7 cut(s) 514, 636, 973, 1106, 2724, 2763, 3707
DraI TTTAAA 5 cut(s) 465, 724, 2097, 2281, 2989
DraIII CACNNNGTG 1 cut(s) 1828
EaeI YGGCCR 4 cut(s) 919, 1411, 1908, 1929
EagI CGGCCG 1 cut(s) 1929
Eam1104I CTCTTC 9 cut(s) 507, 675, 1314, 2646, 2952, 3151, 3490, 3546, 3762
EarI CTCTTC 9 cut(s) 507, 675, 1314, 2646, 2952, 3151, 3490, 3546, 3762
EciI GGCGGA 1 cut(s) 1412
Ecl136II GAGCTC 3 cut(s) 1428, 2487, 3638
EclXI CGGCCG 1 cut(s) 1929
Eco105I TACGTA 1 cut(s) 1981
Eco130I CCWWGG 3 cut(s) 2520, 3132, 3682
Eco24I GRGCYC 3 cut(s) 1430, 2489, 3640
Eco31I GGTCTC 1 cut(s) 3153
Eco32I GATATC 1 cut(s) 3073
Eco47I GGWCC 4 cut(s) 1583, 2377, 2433, 3592
Eco47III AGCGCT 1 cut(s) 3220
Eco52I CGGCCG 1 cut(s) 1929
Eco53kI GAGCTC 3 cut(s) 1428, 2487, 3638
Eco57I CTGAAG 4 cut(s) 611, 699, 1572, 3065
Eco81I CCTNAGG 1 cut(s) 3696
Eco88I CYCGRG 2 cut(s) 1509, 3174
Eco91I GGTNACC 1 cut(s) 2078
EcoICRI GAGCTC 3 cut(s) 1428, 2487, 3638
EcoO109I RGGNCCY 1 cut(s) 1583
EcoO65I GGTNACC 1 cut(s) 2078
EcoRI GAATTC 1 cut(s) 1792
EcoRII CCWGG 7 cut(s) 1655, 1910, 2435, 2980, 3062, 3300, 3629
EcoRV GATATC 1 cut(s) 3073
EcoT14I CCWWGG 3 cut(s) 2520, 3132, 3682
EcoT22I ATGCAT 1 cut(s) 76
EcoT38I GRGCYC 3 cut(s) 1430, 2489, 3640
ErhI CCWWGG 3 cut(s) 2520, 3132, 3682
Esp3I CGTCTC 1 cut(s) 3456
FalI AAGNNNNNCTT 6 cut(s) 2601, 2633, 3405, 3437, 3654, 3686
FaqI GGGAC 2 cut(s) 468, 1596
FauI CCCGC 2 cut(s) 3718, 3737
FbaI TGATCA 1 cut(s) 1639
FblI GTMKAC 2 cut(s) 3036, 3729
FokI GGATG 9 cut(s) 134, 188, 2636, 2765, 2933, 2960, 2988, 3221, 3478
FriOI GRGCYC 3 cut(s) 1430, 2489, 3640
FspBI CTAG 7 cut(s) 435, 1515, 2130, 2697, 2784, 3507, 3772
GlaI GCGC 1 cut(s) 3220
GsaI CCCAGC 1 cut(s) 3526
GsuI CTGGAG 1 cut(s) 117
HaeII RGCGCY 1 cut(s) 3222
HaeIII GGCC 5 cut(s) 921, 1413, 1910, 1931, 3185
HapII CCGG 7 cut(s) 1932, 2104, 3277, 3443, 3473, 3586, 3739
HgaI GACGC 3 cut(s) 2099, 3179, 3438
HhaI GCGC 1 cut(s) 3221
Hin1I GRCGYC 1 cut(s) 457
Hin6I GCGC 1 cut(s) 3219
HinP1I GCGC 1 cut(s) 3219
HincII GTYRAC 1 cut(s) 3730
HindII GTYRAC 1 cut(s) 3730
HindIII AAGCTT 2 cut(s) 448, 3615
HpaII CCGG 7 cut(s) 1932, 2104, 3277, 3443, 3473, 3586, 3739
HphI GGTGA 9 cut(s) 836, 1540, 2423, 2432, 2885, 2990, 3074, 3382, 3432
Hpy166II GTNNAC 7 cut(s) 1715, 2019, 2188, 2953, 3037, 3478, 3730
Hpy8I GTNNAC 7 cut(s) 1715, 2019, 2188, 2953, 3037, 3478, 3730
Hpy99I CGWCG 5 cut(s) 535, 3150, 3254, 3738, 3765
HpyAV CCTTC 9 cut(s) 131, 253, 311, 586, 1301, 1596, 2135, 2469, 3280
HpyCH4III ACNGT 8 cut(s) 938, 1826, 2209, 2233, 2897, 3041, 3250, 3734
HpyCH4IV ACGT 6 cut(s) 91, 457, 1717, 1882, 1980, 3343
HpySE526I ACGT 6 cut(s) 91, 457, 1717, 1882, 1980, 3343
Hsp92I GRCGYC 1 cut(s) 457
HspAI GCGC 1 cut(s) 3219
Ksp22I TGATCA 1 cut(s) 1639
LguI GCTCTTC 1 cut(s) 3762
LmnI GCTCC 9 cut(s) 109, 136, 541, 1852, 2107, 2848, 2971, 3058, 3741
Lsp1109I GCAGC 9 cut(s) 220, 1117, 3208, 3211, 3214, 3228, 3567, 3586, 3613
LweI GCATC 2 cut(s) 361, 3408
MaeI CTAG 7 cut(s) 435, 1515, 2130, 2697, 2784, 3507, 3772
MaeII ACGT 6 cut(s) 91, 457, 1717, 1882, 1980, 3343
MaeIII GTNAC 8 cut(s) 274, 345, 702, 1976, 2078, 2227, 2891, 3244
MbiI CCGCTC 1 cut(s) 3744
MflI RGATCY 5 cut(s) 497, 977, 1478, 1892, 2685
MhlI GDGCHC 5 cut(s) 1430, 2489, 3390, 3480, 3640
MlsI TGGCCA 2 cut(s) 921, 1910
MluNI TGGCCA 2 cut(s) 921, 1910
MlyI GAGTC 1 cut(s) 692
MmeI TCCRAC 1 cut(s) 3118
Mox20I TGGCCA 2 cut(s) 921, 1910
Mph1103I ATGCAT 1 cut(s) 76
MscI TGGCCA 2 cut(s) 921, 1910
MslI CAYNNNNRTG 1 cut(s) 2067
Msp20I TGGCCA 2 cut(s) 921, 1910
MspA1I CMGCKG 2 cut(s) 2399, 3236
MspI CCGG 7 cut(s) 1932, 2104, 3277, 3443, 3473, 3586, 3739
Mva1269I GAATGC 3 cut(s) 729, 1185, 1537
MvaI CCWGG 7 cut(s) 1657, 1912, 2437, 2982, 3064, 3302, 3631
MvnI CGCG 1 cut(s) 3713
NciI CCSGG 4 cut(s) 2105, 3277, 3473, 3587
NcoI CCATGG 1 cut(s) 3132
NlaIV GGNNCC 3 cut(s) 1584, 2694, 3702
NmeAIII GCCGAG 2 cut(s) 3129, 3211
NmuCI GTSAC 1 cut(s) 2891
NsiI ATGCAT 1 cut(s) 76
NspI RCATGY 1 cut(s) 3572
NspV TTCGAA 1 cut(s) 813
PaeI GCATGC 1 cut(s) 3572
PagI TCATGA 4 cut(s) 313, 1834, 3337, 3643
PciSI GCTCTTC 1 cut(s) 3762
PctI GAATGC 3 cut(s) 729, 1185, 1537
PflMI CCANNNNNTGG 1 cut(s) 3205
PinAI ACCGGT 1 cut(s) 3442
PleI GAGTC 1 cut(s) 691
PpsI GAGTC 1 cut(s) 691
Ppu21I YACGTR 1 cut(s) 1981
PpuMI RGGWCCY 1 cut(s) 1583
PshBI ATTAAT 1 cut(s) 2223
PsiI TTATAA 1 cut(s) 1362
Psp124BI GAGCTC 3 cut(s) 1430, 2489, 3640
Psp5II RGGWCCY 1 cut(s) 1583
Psp6I CCWGG 7 cut(s) 1655, 1910, 2435, 2980, 3062, 3300, 3629
PspEI GGTNACC 1 cut(s) 2078
PspFI CCCAGC 1 cut(s) 3522
PspGI CCWGG 7 cut(s) 1655, 1910, 2435, 2980, 3062, 3300, 3629
PspN4I GGNNCC 3 cut(s) 1584, 2694, 3702
PspPI GGNCC 4 cut(s) 1583, 2377, 2433, 3592
PspPPI RGGWCCY 1 cut(s) 1583
PsrI GAACNNNNNNTAC 4 cut(s) 330, 362, 1089, 1121
PstI CTGCAG 2 cut(s) 1135, 2497
PsuI RGATCY 5 cut(s) 497, 977, 1478, 1892, 2685
PvuII CAGCTG 1 cut(s) 2399
RsaI GTAC 7 cut(s) 515, 637, 974, 1107, 2725, 2764, 3708
RsaNI GTAC 7 cut(s) 514, 636, 973, 1106, 2724, 2763, 3707
RseI CAYNNNNRTG 1 cut(s) 2067
SacI GAGCTC 3 cut(s) 1430, 2489, 3640
SalI GTCGAC 1 cut(s) 3728
SapI GCTCTTC 1 cut(s) 3762
Sau96I GGNCC 4 cut(s) 1583, 2377, 2433, 3592
SchI GAGTC 1 cut(s) 692
SduI GDGCHC 5 cut(s) 1430, 2489, 3390, 3480, 3640
SfaNI GCATC 2 cut(s) 361, 3408
SfcI CTRYAG 2 cut(s) 1131, 2493
SfuI TTCGAA 1 cut(s) 813
SinI GGWCC 4 cut(s) 1583, 2377, 2433, 3592
SmiMI CAYNNNNRTG 1 cut(s) 2067
SmlI CTYRAG 6 cut(s) 1211, 1220, 1556, 2796, 3411, 3660
SmoI CTYRAG 6 cut(s) 1211, 1220, 1556, 2796, 3411, 3660
SnaBI TACGTA 1 cut(s) 1981
SphI GCATGC 1 cut(s) 3572
SsiI CCGC 9 cut(s) 279, 1397, 2431, 3231, 3234, 3665, 3711, 3744, 3748
SspI AATATT 3 cut(s) 563, 652, 1811
SspMI CTAG 7 cut(s) 435, 1515, 2130, 2697, 2784, 3507, 3772
SstI GAGCTC 3 cut(s) 1430, 2489, 3640
StyI CCWWGG 3 cut(s) 2520, 3132, 3682
TaaI ACNGT 8 cut(s) 938, 1826, 2209, 2233, 2897, 3041, 3250, 3734
TaiI ACGT 6 cut(s) 94, 460, 1720, 1885, 1983, 3346
TaqI TCGA 7 cut(s) 813, 1013, 1816, 2002, 3310, 3729, 3763
TatI WGTACW 1 cut(s) 513
TauI GCSGC 2 cut(s) 3233, 3236
TseFI GTSAC 1 cut(s) 2891
TseI GCWGC 9 cut(s) 233, 1130, 3216, 3221, 3224, 3227, 3555, 3599, 3626
Tsp45I GTSAC 1 cut(s) 2891
TspGWI ACGGA 3 cut(s) 134, 2369, 3644
Van91I CCANNNNNTGG 1 cut(s) 3205
VneI GTGCAC 1 cut(s) 3476
VpaK11BI GGWCC 4 cut(s) 1583, 2377, 2433, 3592
VspI ATTAAT 1 cut(s) 2223
XapI RAATTY 9 cut(s) 489, 719, 745, 808, 1340, 1792, 1799, 2335, 2498
XceI RCATGY 1 cut(s) 3572
XcmI CCANNNNNNNNNTGG 1 cut(s) 2443
XmiI GTMKAC 2 cut(s) 3036, 3729
XspI CTAG 7 cut(s) 435, 1515, 2130, 2697, 2784, 3507, 3772
ZraI GACGTC 1 cut(s) 458
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.