pycom05g05480

GAG-pre-integrase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000703
Physical Location & Seq
Reverse (-)
6609744 .. 6610448
705 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g05480.1

Sequence Viewer

Length: 705 bp
ATGACACAAAAGATTAGGACCTGTGAATTATCGAGATTTACTCAAGTTGTCAAAGAAGTCTTAAGTTCATGGACTACCATCACTAAGTGGACTACTAGAATTCTTGAGCTTATGCATATGTATTTGGTAGGTCCAATGTCAACTCAAAGCATTGGTGGTAAGAAAGACATTCTTGTTTTAGTTGATGACTTTTCAAGGTATGCTTGGGTAGATTTTCTTGCTACTAAATCTGATACATTTGATTCATTTTCTAAAATTTGTTCTAAGATTCAAAATGAGAAAAATGATAAAGTAACACGTCTTAGAACAGATCATGGGACTGAATTTGAAAATGCATCATTTCATGATTTTTGCAATACTAATGGAATTGCTCATGAGTTTTCAACTCCAATAACACCACAACAAAATGGTGTTGTTGAACGTAAGAATAGGGTACTAATTGAGATGGGAAGAGTCATGCTAAATCCTGCAAATCTAGCCAATCATTTCTGGGCTGAAGTATTAGTACAACATGTTATACTTGGAACAAAGTTTATCTCAGACCAGGTACAAACACCACACCATATGAGTTCTGGAAAGGTAAGAAATCCAATGCAAGTCATCTTTGAATCTTTGGTAGTATTTGCTACATCTATAAGGATAGAGATTTGGTGGGAAAGTTTGATGCCCGTAGTGATACCGGTACTTTTCTTGGTTACTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
gag_pre-integrs

Protein Analysis

235

Amino Acids

26.85

Weight (kDa)

7.79

Isoelectric Point (pI)

24.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
rve PF00665 50 - 133 5.1e-11 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 99, 255, 323
AcuI CTGAAG 1 cut(s) 516
AdeI CACNNNGTG 1 cut(s) 87
AfaI GTAC 4 cut(s) 435, 507, 549, 684
AflII CTTAAG 1 cut(s) 61
AflIII ACRYGT 2 cut(s) 296, 511
AgeI ACCGGT 1 cut(s) 679
AgsI TTSAA 6 cut(s) 195, 272, 329, 384, 419, 608
AjiI CACGTC 1 cut(s) 299
AjnI CCWGG 1 cut(s) 543
AluBI AGCT 1 cut(s) 109
AluI AGCT 1 cut(s) 109
ApoI RAATTY 3 cut(s) 99, 255, 323
AsiGI ACCGGT 1 cut(s) 679
AspS9I GGNCC 2 cut(s) 18, 131
AvaII GGWCC 2 cut(s) 18, 131
BarI GAAGNNNNNNTAC 2 cut(s) 489, 521
BccI CCATC 2 cut(s) 86, 439
BciT130I CCWGG 1 cut(s) 545
BfaI CTAG 2 cut(s) 96, 476
BfrI CTTAAG 1 cut(s) 61
Bme1390I CCNGG 1 cut(s) 545
Bme18I GGWCC 2 cut(s) 18, 131
BmgBI CACGTC 1 cut(s) 299
BmgT120I GGNCC 2 cut(s) 18, 131
BmrFI CCNGG 1 cut(s) 545
BmsI GCATC 2 cut(s) 344, 654
BplI GAGNNNNNCTC 2 cut(s) 25, 57
BpuEI CTTGAG 2 cut(s) 27, 125
BsaWI WCCGGW 1 cut(s) 679
Bse118I RCCGGY 1 cut(s) 679
BseBI CCWGG 1 cut(s) 545
BseMII CTCAG 1 cut(s) 552
BshTI ACCGGT 1 cut(s) 679
BsiSI CCGG 1 cut(s) 680
BslFI GGGAC 1 cut(s) 331
BsmFI GGGAC 1 cut(s) 331
Bsp143I GATC 1 cut(s) 310
BspCNI CTCAG 1 cut(s) 551
BspHI TCATGA 2 cut(s) 343, 373
BspTI CTTAAG 1 cut(s) 61
BsrFI RCCGGY 1 cut(s) 679
BssAI RCCGGY 1 cut(s) 679
BssMI GATC 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 545
Bst6I CTCTTC 1 cut(s) 445
BstAFI CTTAAG 1 cut(s) 61
BstDEI CTNAG 4 cut(s) 84, 264, 302, 538
BstKTI GATC 1 cut(s) 313
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 1 cut(s) 476
BstNI CCWGG 1 cut(s) 545
BstNSI RCATGY 1 cut(s) 515
BstSCI CCNGG 1 cut(s) 543
BtrI CACGTC 1 cut(s) 299
CciI TCATGA 2 cut(s) 343, 373
Cfr10I RCCGGY 1 cut(s) 679
Cfr13I GGNCC 2 cut(s) 18, 131
CsiI ACCWGGT 1 cut(s) 543
Csp6I GTAC 4 cut(s) 434, 506, 548, 683
CspAI ACCGGT 1 cut(s) 679
CviAII CATG 6 cut(s) 69, 314, 344, 374, 457, 512
CviJI RGCY 3 cut(s) 109, 479, 494
CviKI_1 RGCY 3 cut(s) 109, 479, 494
CviQI GTAC 4 cut(s) 434, 506, 548, 683
DdeI CTNAG 4 cut(s) 84, 264, 302, 538
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
DraIII CACNNNGTG 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 445
EarI CTCTTC 1 cut(s) 445
Eco47I GGWCC 2 cut(s) 18, 131
Eco57I CTGAAG 1 cut(s) 516
EcoO109I RGGNCCY 1 cut(s) 18
EcoRI GAATTC 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 543
EcoT22I ATGCAT 2 cut(s) 117, 337
FaeI CATG 6 cut(s) 72, 317, 347, 377, 460, 515
FalI AAGNNNNNCTT 4 cut(s) 156, 188, 187, 219
FaqI GGGAC 1 cut(s) 331
FatI CATG 6 cut(s) 68, 313, 343, 373, 456, 511
FauNDI CATATG 2 cut(s) 117, 564
FspBI CTAG 2 cut(s) 96, 476
HapII CCGG 1 cut(s) 680
Hin1II CATG 6 cut(s) 72, 317, 347, 377, 460, 515
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HinfI GANTC 4 cut(s) 242, 268, 453, 608
HpaII CCGG 1 cut(s) 680
Hpy166II GTNNAC 2 cut(s) 90, 141
Hpy188I TCNGA 2 cut(s) 232, 541
Hpy188III TCNNGA 5 cut(s) 33, 104, 344, 374, 573
Hpy8I GTNNAC 2 cut(s) 90, 141
HpyCH4IV ACGT 2 cut(s) 298, 421
HpyCH4V TGCA 5 cut(s) 115, 335, 354, 470, 595
HpyF10VI GCNNNNNNNGC 1 cut(s) 476
HpyF3I CTNAG 4 cut(s) 84, 264, 302, 538
HpySE526I ACGT 2 cut(s) 298, 421
Hsp92II CATG 6 cut(s) 72, 317, 347, 377, 460, 515
Kzo9I GATC 1 cut(s) 310
LpnPI CCDG 7 cut(s) 34, 475, 480, 530, 557, 558, 693
LweI GCATC 2 cut(s) 344, 654
MabI ACCWGGT 1 cut(s) 543
MaeI CTAG 2 cut(s) 96, 476
MaeII ACGT 2 cut(s) 298, 421
MaeIII GTNAC 2 cut(s) 292, 694
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 1 cut(s) 462
MluCI AATT 6 cut(s) 26, 99, 255, 323, 366, 438
MlyI GAGTC 1 cut(s) 462
Mph1103I ATGCAT 2 cut(s) 117, 337
MseI TTAA 2 cut(s) 62, 703
MspCI CTTAAG 1 cut(s) 61
MspI CCGG 1 cut(s) 680
MspR9I CCNGG 1 cut(s) 545
MvaI CCWGG 1 cut(s) 545
MwoI GCNNNNNNNGC 1 cut(s) 476
NdeI CATATG 2 cut(s) 117, 564
NdeII GATC 1 cut(s) 310
NlaIII CATG 6 cut(s) 72, 317, 347, 377, 460, 515
NsiI ATGCAT 2 cut(s) 117, 337
NspI RCATGY 1 cut(s) 515
PagI TCATGA 2 cut(s) 343, 373
PciI ACATGT 1 cut(s) 511
PfeI GAWTC 3 cut(s) 242, 268, 608
PinAI ACCGGT 1 cut(s) 679
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
PpuMI RGGWCCY 1 cut(s) 18
PscI ACATGT 1 cut(s) 511
Psp5II RGGWCCY 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 543
PspGI CCWGG 1 cut(s) 543
PspPI GGNCC 2 cut(s) 18, 131
PspPPI RGGWCCY 1 cut(s) 18
RsaI GTAC 4 cut(s) 435, 507, 549, 684
RsaNI GTAC 4 cut(s) 434, 506, 548, 683
SaqAI TTAA 2 cut(s) 62, 703
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 2 cut(s) 18, 131
SchI GAGTC 1 cut(s) 462
ScrFI CCNGG 1 cut(s) 545
SetI ASST 8 cut(s) 23, 111, 133, 200, 301, 424, 549, 582
SexAI ACCWGGT 1 cut(s) 543
SfaNI GCATC 2 cut(s) 344, 654
SinI GGWCC 2 cut(s) 18, 131
SmlI CTYRAG 3 cut(s) 42, 61, 104
SmoI CTYRAG 3 cut(s) 42, 61, 104
Sse9I AATT 6 cut(s) 26, 99, 255, 323, 366, 438
SspMI CTAG 2 cut(s) 96, 476
StyD4I CCNGG 1 cut(s) 543
TaiI ACGT 2 cut(s) 301, 424
TaqI TCGA 1 cut(s) 32
TasI AATT 6 cut(s) 26, 99, 255, 323, 366, 438
TatI WGTACW 1 cut(s) 505
TfiI GAWTC 3 cut(s) 242, 268, 608
Tru1I TTAA 2 cut(s) 62, 703
Tru9I TTAA 2 cut(s) 62, 703
TspDTI ATGAA 3 cut(s) 57, 234, 332
Vha464I CTTAAG 1 cut(s) 61
VpaK11BI GGWCC 2 cut(s) 18, 131
XapI RAATTY 3 cut(s) 99, 255, 323
XceI RCATGY 1 cut(s) 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XspI CTAG 2 cut(s) 96, 476
Zsp2I ATGCAT 2 cut(s) 117, 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.