FvH4_4g18201

spliceosomal complex assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000703
Physical Location & Seq
Reverse (-)
22166382 .. 22173038
6657 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18201.t1

Sequence Viewer

Length: 3138 bp
ATGAAAGATGATGAATCTATTGATGATTTTTATGCTCGTTTTACTGATCTTTCTAGTTTATGTGAAAGCTTGGGACGTCCTTTAAAAGAATCTGATATTGTCAGGAAAATTTTGAGATCTCTTCCAAAGAAGTACAGGATAAAAAAGACGACGATTCAGGAGCAATACAACTTGAATGAATATTCAGTAGATTTGTTGATCGGAAATCTGAAGGCTTGGGAAATGGAACTTGAAGATGACGAAAAACCTAAAGGTACAAGTTCAAAAAATATTGCTTTGAATGTCTCTAAGAAGTCTGAAGAGTCTGAGCATAATGAGTTGTTATTTTTGACTAAAGAATTTAAACGCATTCTGAATCAAAGAAATTCAAAACCTAATGTGCGAAACAATTTCAATTCTAATAAGAGGTTTAATGGAAATAATAAGAATTTCGAAAAACAAGGTGATAATTCGTTTCAAAAGAAAAATGAAACTGGTAATAGTAGTAATGGAAATTATCGGGGTCAAAAGGGTCGTTGTTATGAATGTGGTGGCTATGGCCACATTGCCACTGACTGTGCAAATAGAAAGTCTGATACAGGGAAAAAGGCGTACAGATCCTCATGGAGTGATGAGGAAGAAGATGATTCCTCGAAAGGAAACAAAACTTTTGCTCTTGTGTCTTCTTTTTCTCTTGATTATTCAGATTCAGATGATGAGTTTGAGGAAGATGATGAACTAATGGTACATCATAAGAAGGTTCTAAAGGCTGCAGAACTGGTATCTGAAAAAAATGAGGCTTTGAAGTTTCAAGTAGGAATGCTACAAGAAGAAAAACAAAAATGGGAACTTGAGAAACTTGAGTTAATGAGAAAATGTGAGGAGGATCATAACATGAGTAGTGAAGATTGGAAAGTGGAAAGAAAACGCCTTCTTGCAAAGATTCAGATACTCCAAGAAGAGGTAAATGAAAAAGATAAATTTATTTTGCAACTAACTTATAAAGTTGATAAATTACAGGAATCTCTTGAAAAGGCGGAAGACAAGTTCGACCAGTTCAAAATGAGCTCTCAATCTCTGACAAGTTCTGTTGAAGAAACAATGGAACTATGGCACAAACGACTTGGTCACTTAAATTTTCAGGACTTGCTTCAACTGTCCACCCAAAAATTGGTAAGAGGTTTACCTAACTTAAGTGGTCGTACTGATGTTGTTTGCGGAGGTTGCAAAACAGGGAAACAAACCCGAGCTAGTCATAAAGTGGTGAATGCTTCATCATCAACTCAACCTCTTGAGTTGCTTCACATGGATTTGATGGGACCTTCAGCGACATCAAGTATGGCAGGTAAAGAATACATTTTTGTTGTTGTTGATGATCATTCTCGTTTTGCCTGGGTTGATTTCTTAAAAGATAAAAGTGAGACATTTAGTTCTTTTTCCACCTTGAGTAAACGTCTTTTAGTTGAAAAACAACAAGAAAATTATCAAATTGCCAAGATTAGGTCAGATAATGGAACTGAGTTTAAGAATTCTAAATTTGCTAAATATTGTCGAGATCACGGTGTGTTTCATGAGTTTTCAGCTCCTATAACTCTACAGCAAAATGGAATTGTTGAACGTAAGAACAGATCTCTCTTAGACATGGCCAGGGTCATGCTTCATTCGGCCGGACTTAGTGAAAACTTCTGGGCTGAGGCTATTAACACAGCTTGTTACGTAGGAAATCGTGTTCTTCTTCGATCAGGAACAAAGTCCACTCCTTATGAACTATGGAAAGGTAAAAAGCCAAATGTAAGTCATTTTCATGTGTTTGGTAACCCTTGCTATGTTTTAAAAGACCGGGAGCGTCTTGCCAAGTTTGCACCTAGAGCAGATGAAGGTATTTTTCTTGGTTATTCTCTTGATAGTCGTGCTTACAGAGTTTACAACAAAGAAACTAAAACAGTTATGGATTCAATTAATGTTACAGTTGATGATAATGCAGATTTGTCTGTTGTTAATGATTCAGATGTGTTTAAATCAAATGACAATAGTAGTTCTGTGCAAGAAAAGACTAATGAAGTTGATAAAAATTTACAACCTGCTACAAAATATAGAACTGGTTTTAAGAATAAGATGGATGAGCATGGTACTATCACTCGGAATAAAGCTAGATTGGTGGCTCAAGGTTATTCACAGGTTGAGGGATTGGATTTTGATGAAACCTTTGCTCCTGTGGCTCGGTTGGAATCTATAAGACTTCTATTAGCTATTGCGTGTCACCGTCAGTTCAAACTTTTTCAAATGGATGTTAAAACTGCATTTCTGAATGGGGATGTTCAAGAAGAGGTGTTTGTGGAGCAACCACCTGGCTTTAAAGATATTCACCATCCTGAGTATGTTTGGAAACTAAAAAAGGCTGTCTACGGTCTGAAGCAAGCTCCTCGTGCCTGGTATGATATCTTGATTCACCAAGATTTTGATCTTCTGCCGAGATCATCTTTTAGAGTTTTCGTCGTCATGGTTGGAGTTCGTCGTTCAAGAAGAGGCATGCAAGAAGCTCGGGCTGAGGCCGAGCGTCTGAACCAGGAGATGGATGAACGCCACGCTGCTGCTGCCGCCGCTGCTCCTGTCACTGTCGGCGACCTCACACAGGCCTTCTCTCGGCTTGGTGTTCATCTGCAAGCCCAGGTTGATCGAATCCACCAAGTTCATCTCTTACATCATGAACGTATTACTGCTCAATTATCCCGACTGGAACTTGCGGTTGGTGTGCTCACCGATCTCGGTGTGGAATCTCAAGAGATGCTTTCCAGGCAGGTTGTTTCGCCGGTTGCTTCCCCTGCCCTAGCCCCAGTGCACTCCTCATCGTCATCCTCTACTTTCTCTCCCTCTGACAACGCACAGCTTTCTTCTCATCGTCCTCCTCTTCCTCCTCTTCCTCCTCTTCCTCTGGCTGGCAGCTCTCTCTGGCATGCCATTGCTTTGGACCGAGAGGAACAAGCTGCCAGGGAAACACAAGCTTCTTTGGCTGCATGGCGAGCTCATCATGACCATCTCCGTTCTCAAGCGGTTCTTCCCCATGCGGATGGAGCCGTGCCTCAGGTTCCTGCACCTGCGAATGATGATCTTGCCGGCCGTCGCCGGAGCGGGCGGTTGGCTCTTCGTCGAAGTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve zf-CCHC

Protein Analysis

1046

Amino Acids

118.61

Weight (kDa)

7.65

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 1 - 80 7.6e-09 gag-polypeptide of LTR copia-type
gag_pre-integrs PF13976 350 - 407 4.9e-13 GAG-pre-integrase domain
rve PF00665 421 - 522 7.4e-12 Integrase core domain
SH3_retrovirus PF25597 600 - 657 2.4e-16 Retroviral polymerase SH3-like domain
RVT_2 PF07727 693 - 808 7.1e-42 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 981
AarI CACCTGC 1 cut(s) 3082
AatII GACGTC 1 cut(s) 79
Acc36I ACCTGC 4 cut(s) 1313, 2068, 2767, 3082
AccB7I CCANNNNNTGG 2 cut(s) 1150, 2551
AccBSI CCGCTC 1 cut(s) 3108
AccI GTMKAC 1 cut(s) 2382
AciI CCGC 9 cut(s) 1016, 1197, 2577, 2580, 2723, 3029, 3044, 3108, 3112
AclWI GGATC 2 cut(s) 591, 873
AcoI YGGCCR 4 cut(s) 538, 1623, 1644, 3094
AcsI RAATTY 9 cut(s) 108, 338, 364, 427, 959, 1114, 1507, 1514, 2050
AcuI CTGAAG 4 cut(s) 230, 318, 1287, 2411
AcyI GRCGYC 1 cut(s) 76
AdeI CACNNNGTG 1 cut(s) 1543
AfaI GTAC 6 cut(s) 134, 256, 593, 726, 1183, 2110
AfiI CCNNNNNNNGG 7 cut(s) 940, 1150, 1479, 2551, 2611, 2622, 2915
AflII CTTAAG 1 cut(s) 1171
AjnI CCWGG 8 cut(s) 1370, 1625, 2326, 2408, 2544, 2646, 2771, 2966
AjuI GAANNNNNNNTTGG 2 cut(s) 2709, 2741
AloI GAACNNNNNNTCC 4 cut(s) 1010, 1042, 1692, 1724
Alw21I GWGCWC 4 cut(s) 1049, 2736, 2820, 3004
Alw26I GTCTC 2 cut(s) 289, 1394
Alw44I GTGCAC 1 cut(s) 2816
AlwI GGATC 2 cut(s) 591, 873
Ama87I CYCGRG 2 cut(s) 1224, 2520
AoxI GGCC 6 cut(s) 538, 1623, 1644, 2529, 2613, 3094
ApaLI GTGCAC 1 cut(s) 2816
ApeKI GCWGC 8 cut(s) 749, 2567, 2570, 2573, 2582, 2919, 2963, 2990
ApoI RAATTY 9 cut(s) 108, 338, 364, 427, 959, 1114, 1507, 1514, 2050
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
AseI ATTAAT 1 cut(s) 1938
AspS9I GGNCC 2 cut(s) 1298, 2947
AsuC2I CCSGG 1 cut(s) 1820
AsuHPI GGTGA 6 cut(s) 455, 1255, 2231, 2336, 2420, 2728
AsuII TTCGAA 1 cut(s) 432
AvaI CYCGRG 2 cut(s) 1224, 2520
AvaII GGWCC 2 cut(s) 1298, 2947
AxyI CCTNAGG 1 cut(s) 3060
BaeGI GKGCMC 1 cut(s) 2820
BalI TGGCCA 2 cut(s) 540, 1625
BanII GRGCYC 2 cut(s) 1049, 3004
BauI CACGAG 1 cut(s) 2403
BbsI GAAGAC 2 cut(s) 654, 1026
Bbv12I GWGCWC 4 cut(s) 1049, 2736, 2820, 3004
BbvCI CCTCAGC 2 cut(s) 1671, 2526
BbvI GCAGC 8 cut(s) 736, 2554, 2557, 2560, 2569, 2931, 2950, 2977
BccI CCATC 6 cut(s) 1288, 2089, 2355, 2545, 3021, 3041
BceAI ACGGC 2 cut(s) 3038, 3081
BcgI CGANNNNNNTGC 4 cut(s) 2384, 2418, 2501, 2535
BciT130I CCWGG 8 cut(s) 1372, 1627, 2328, 2410, 2546, 2648, 2773, 2968
BclI TGATCA 1 cut(s) 1354
BcnI CCSGG 1 cut(s) 1820
BcoDI GTCTC 2 cut(s) 289, 1394
BfaI CTAG 6 cut(s) 54, 1230, 1845, 2130, 2807, 3136
BfmI CTRYAG 2 cut(s) 750, 1574
BfrI CTTAAG 1 cut(s) 1171
BfuAI ACCTGC 4 cut(s) 1313, 2068, 2767, 3082
BglII AGATCT 2 cut(s) 116, 1607
Bme1390I CCNGG 9 cut(s) 1372, 1627, 1820, 2328, 2410, 2546, 2648, 2773, 2968
Bme18I GGWCC 2 cut(s) 1298, 2947
BmeT110I CYCGRG 2 cut(s) 1224, 2520
BmgT120I GGNCC 2 cut(s) 1298, 2947
BmiI GGNNCC 3 cut(s) 1299, 3052, 3066
BmrFI CCNGG 9 cut(s) 1372, 1627, 1820, 2328, 2410, 2546, 2648, 2773, 2968
BmrI ACTGGG 1 cut(s) 2807
BmsI GCATC 1 cut(s) 2754
BmuI ACTGGG 1 cut(s) 2807
BpiI GAAGAC 2 cut(s) 654, 1026
Bpu10I CCTNAGC 2 cut(s) 1671, 2526
Bpu14I TTCGAA 1 cut(s) 432
BpuEI CTTGAG 7 cut(s) 851, 860, 1292, 1444, 2127, 2742, 3009
BpuMI CCSGG 1 cut(s) 1820
BsaAI YACGTR 1 cut(s) 1696
BsaBI GATNNNNATC 1 cut(s) 2439
BsaHI GRCGYC 1 cut(s) 76
BsaJI CCNNGG 4 cut(s) 1371, 1626, 2646, 2967
BsaXI ACNNNNNCTCC 4 cut(s) 2173, 2203, 2830, 2860
Bsc4I CCNNNNNNNGG 7 cut(s) 940, 1150, 1479, 2551, 2611, 2622, 2915
Bse118I RCCGGY 2 cut(s) 2788, 3092
Bse1I ACTGG 6 cut(s) 478, 762, 1033, 2083, 2718, 2813
Bse21I CCTNAGG 1 cut(s) 3060
Bse3DI GCAATG 2 cut(s) 543, 2937
Bse8I GATNNNNATC 1 cut(s) 2439
BseBI CCWGG 8 cut(s) 1372, 1627, 2328, 2410, 2546, 2648, 2773, 2968
BseDI CCNNGG 4 cut(s) 1371, 1626, 2646, 2967
BseGI GGATG 7 cut(s) 2104, 2272, 2299, 2347, 2560, 2831, 3052
BseJI GATNNNNATC 1 cut(s) 2439
BseLI CCNNNNNNNGG 7 cut(s) 940, 1150, 1479, 2551, 2611, 2622, 2915
BseMI GCAATG 2 cut(s) 543, 2937
BseMII CTCAG 6 cut(s) 297, 1488, 1662, 2343, 2517, 3074
BseNI ACTGG 6 cut(s) 478, 762, 1033, 2083, 2718, 2813
BseRI GAGGAG 6 cut(s) 875, 2391, 2812, 2874, 2883, 2892
BseSI GKGCMC 1 cut(s) 2820
BseX3I CGGCCG 2 cut(s) 1644, 3094
BseXI GCAGC 8 cut(s) 736, 2554, 2557, 2560, 2569, 2931, 2950, 2977
BsgI GTGCAG 1 cut(s) 3054
Bsh1285I CGRYCG 2 cut(s) 1647, 3097
BshFI GGCC 6 cut(s) 540, 1625, 1646, 2531, 2615, 3096
BsiEI CGRYCG 2 cut(s) 1647, 3097
BsiHKAI GWGCWC 4 cut(s) 1049, 2736, 2820, 3004
BsiHKCI CYCGRG 2 cut(s) 1224, 2520
BsiSI CCGG 5 cut(s) 1647, 1819, 2789, 3093, 3103
BslFI GGGAC 2 cut(s) 87, 1311
BslI CCNNNNNNNGG 7 cut(s) 940, 1150, 1479, 2551, 2611, 2622, 2915
BsmAI GTCTC 2 cut(s) 289, 1394
BsmFI GGGAC 2 cut(s) 87, 1311
BsmI GAATGC 3 cut(s) 348, 804, 1252
BsnI GGCC 6 cut(s) 540, 1625, 1646, 2531, 2615, 3096
BsoBI CYCGRG 2 cut(s) 1224, 2520
Bsp119I TTCGAA 1 cut(s) 432
Bsp1286I GDGCHC 4 cut(s) 1049, 2736, 2820, 3004
BspACI CCGC 9 cut(s) 1016, 1197, 2577, 2580, 2723, 3029, 3044, 3108, 3112
BspANI GGCC 6 cut(s) 540, 1625, 1646, 2531, 2615, 3096
BspCNI CTCAG 6 cut(s) 298, 1489, 1663, 2344, 2518, 3073
BspHI TCATGA 3 cut(s) 1549, 2683, 3007
BspLI GGNNCC 3 cut(s) 1299, 3052, 3066
BspMAI CTGCAG 1 cut(s) 754
BspMI ACCTGC 4 cut(s) 1313, 2068, 2767, 3082
BspPI GGATC 2 cut(s) 591, 873
BspQI GCTCTTC 1 cut(s) 3126
BspT104I TTCGAA 1 cut(s) 432
BspTI CTTAAG 1 cut(s) 1171
BsrBI CCGCTC 1 cut(s) 3108
BsrDI GCAATG 2 cut(s) 543, 2937
BsrFI RCCGGY 2 cut(s) 2788, 3092
BsrI ACTGG 6 cut(s) 478, 762, 1033, 2083, 2718, 2813
BssAI RCCGGY 2 cut(s) 2788, 3092
BssECI CCNNGG 4 cut(s) 1371, 1626, 2646, 2967
BssNI GRCGYC 1 cut(s) 76
BssSI CACGAG 1 cut(s) 2403
Bst2BI CACGAG 1 cut(s) 2403
Bst2UI CCWGG 8 cut(s) 1372, 1627, 2328, 2410, 2546, 2648, 2773, 2968
Bst4CI ACNGT 8 cut(s) 557, 1137, 1541, 1924, 1948, 2243, 2387, 2596
Bst6I CTCTTC 9 cut(s) 126, 294, 933, 2298, 2497, 2892, 2901, 2910, 3126
BstACI GRCGYC 1 cut(s) 76
BstAFI CTTAAG 1 cut(s) 1171
BstBAI YACGTR 1 cut(s) 1696
BstBI TTCGAA 1 cut(s) 432
BstC8I GCNNGC 8 cut(s) 2397, 2510, 2643, 2917, 2934, 3000, 3094, 3110
BstDEI CTNAG 9 cut(s) 288, 306, 1497, 1615, 1652, 1671, 2352, 2526, 3060
BstEII GGTNACC 1 cut(s) 1793
BstENI CCTNNNNNAGG 1 cut(s) 2609
BstF5I GGATG 7 cut(s) 2104, 2272, 2299, 2347, 2560, 2831, 3052
BstMAI GTCTC 2 cut(s) 289, 1394
BstMCI CGRYCG 2 cut(s) 1647, 3097
BstNI CCWGG 8 cut(s) 1372, 1627, 2328, 2410, 2546, 2648, 2773, 2968
BstNSI RCATGY 2 cut(s) 2512, 2936
BstPI GGTNACC 1 cut(s) 1793
BstSCI CCNGG 9 cut(s) 1370, 1625, 1818, 2326, 2408, 2544, 2646, 2771, 2966
BstSFI CTRYAG 2 cut(s) 750, 1574
BstSLI GKGCMC 1 cut(s) 2820
BstSNI TACGTA 1 cut(s) 1696
BstV1I GCAGC 8 cut(s) 736, 2554, 2557, 2560, 2569, 2931, 2950, 2977
BstV2I GAAGAC 2 cut(s) 654, 1026
BstX2I RGATCY 3 cut(s) 116, 596, 1607
BstXI CCANNNNNNTGG 2 cut(s) 2944, 3047
BstYI RGATCY 3 cut(s) 116, 596, 1607
BstZI CGGCCG 2 cut(s) 1644, 3094
Bsu36I CCTNAGG 1 cut(s) 3060
BsuRI GGCC 6 cut(s) 540, 1625, 1646, 2531, 2615, 3096
BtsCI GGATG 7 cut(s) 2104, 2272, 2299, 2347, 2560, 2831, 3052
BtsIMutI CAGTG 3 cut(s) 549, 2592, 2820
BveI ACCTGC 4 cut(s) 1313, 2068, 2767, 3082
Cac8I GCNNGC 8 cut(s) 2397, 2510, 2643, 2917, 2934, 3000, 3094, 3110
CciI TCATGA 3 cut(s) 1549, 2683, 3007
Cfr10I RCCGGY 2 cut(s) 2788, 3092
Cfr13I GGNCC 2 cut(s) 1298, 2947
CseI GACGC 2 cut(s) 1814, 2525
Csp6I GTAC 6 cut(s) 133, 255, 592, 725, 1182, 2109
CviQI GTAC 6 cut(s) 133, 255, 592, 725, 1182, 2109
DdeI CTNAG 9 cut(s) 288, 306, 1497, 1615, 1652, 1671, 2352, 2526, 3060
DraI TTTAAA 5 cut(s) 84, 343, 1812, 1996, 2335
DraIII CACNNNGTG 1 cut(s) 1543
EaeI YGGCCR 4 cut(s) 538, 1623, 1644, 3094
EagI CGGCCG 2 cut(s) 1644, 3094
Eam1104I CTCTTC 9 cut(s) 126, 294, 933, 2298, 2497, 2892, 2901, 2910, 3126
EarI CTCTTC 9 cut(s) 126, 294, 933, 2298, 2497, 2892, 2901, 2910, 3126
EciI GGCGGA 1 cut(s) 1031
Ecl136II GAGCTC 2 cut(s) 1047, 3002
EclXI CGGCCG 2 cut(s) 1644, 3094
Eco105I TACGTA 1 cut(s) 1696
Eco147I AGGCCT 1 cut(s) 2615
Eco24I GRGCYC 2 cut(s) 1049, 3004
Eco32I GATATC 1 cut(s) 2419
Eco47I GGWCC 2 cut(s) 1298, 2947
Eco52I CGGCCG 2 cut(s) 1644, 3094
Eco53kI GAGCTC 2 cut(s) 1047, 3002
Eco57I CTGAAG 4 cut(s) 230, 318, 1287, 2411
Eco81I CCTNAGG 1 cut(s) 3060
Eco88I CYCGRG 2 cut(s) 1224, 2520
Eco91I GGTNACC 1 cut(s) 1793
EcoICRI GAGCTC 2 cut(s) 1047, 3002
EcoNI CCTNNNNNAGG 1 cut(s) 2609
EcoO109I RGGNCCY 1 cut(s) 1298
EcoO65I GGTNACC 1 cut(s) 1793
EcoRI GAATTC 1 cut(s) 1507
EcoRII CCWGG 8 cut(s) 1370, 1625, 2326, 2408, 2544, 2646, 2771, 2966
EcoRV GATATC 1 cut(s) 2419
EcoT38I GRGCYC 2 cut(s) 1049, 3004
FalI AAGNNNNNCTT 4 cut(s) 2751, 2783, 3018, 3050
FaqI GGGAC 2 cut(s) 87, 1311
FauI CCCGC 1 cut(s) 3101
FbaI TGATCA 1 cut(s) 1354
FblI GTMKAC 1 cut(s) 2382
FokI GGATG 7 cut(s) 2111, 2279, 2306, 2334, 2567, 2818, 3059
FriOI GRGCYC 2 cut(s) 1049, 3004
FspBI CTAG 6 cut(s) 54, 1230, 1845, 2130, 2807, 3136
HaeIII GGCC 6 cut(s) 540, 1625, 1646, 2531, 2615, 3096
HapII CCGG 5 cut(s) 1647, 1819, 2789, 3093, 3103
HgaI GACGC 2 cut(s) 1814, 2525
Hin1I GRCGYC 1 cut(s) 76
HindIII AAGCTT 2 cut(s) 67, 2979
HpaII CCGG 5 cut(s) 1647, 1819, 2789, 3093, 3103
HphI GGTGA 6 cut(s) 455, 1255, 2231, 2336, 2420, 2728
Hpy166II GTNNAC 7 cut(s) 1140, 1163, 1430, 1734, 1903, 2383, 2818
Hpy8I GTNNAC 7 cut(s) 1140, 1163, 1430, 1734, 1903, 2383, 2818
Hpy99I CGWCG 5 cut(s) 154, 2477, 2496, 3102, 3129
HpyAV CCTTC 6 cut(s) 205, 730, 920, 1311, 1850, 2626
HpyCH4III ACNGT 8 cut(s) 557, 1137, 1541, 1924, 1948, 2243, 2387, 2596
HpyCH4IV ACGT 5 cut(s) 76, 1432, 1597, 1695, 2689
HpyF3I CTNAG 9 cut(s) 288, 306, 1497, 1615, 1652, 1671, 2352, 2526, 3060
HpySE526I ACGT 5 cut(s) 76, 1432, 1597, 1695, 2689
Hsp92I GRCGYC 1 cut(s) 76
KroI GCCGGC 1 cut(s) 3092
KroNI GCCGGC 1 cut(s) 3094
Ksp22I TGATCA 1 cut(s) 1354
LguI GCTCTTC 1 cut(s) 3126
LmnI GCTCC 9 cut(s) 160, 1567, 1822, 2194, 2317, 2404, 2590, 3050, 3105
Lsp1109I GCAGC 8 cut(s) 736, 2554, 2557, 2560, 2569, 2931, 2950, 2977
LweI GCATC 1 cut(s) 2754
MaeI CTAG 6 cut(s) 54, 1230, 1845, 2130, 2807, 3136
MaeII ACGT 5 cut(s) 76, 1432, 1597, 1695, 2689
MaeIII GTNAC 6 cut(s) 1106, 1691, 1793, 1942, 2237, 2590
MbiI CCGCTC 1 cut(s) 3108
MflI RGATCY 3 cut(s) 116, 596, 1607
MhlI GDGCHC 4 cut(s) 1049, 2736, 2820, 3004
MlsI TGGCCA 2 cut(s) 540, 1625
MluNI TGGCCA 2 cut(s) 540, 1625
MlyI GAGTC 1 cut(s) 311
MmeI TCCRAC 2 cut(s) 2184, 2464
Mox20I TGGCCA 2 cut(s) 540, 1625
MroNI GCCGGC 1 cut(s) 3092
MscI TGGCCA 2 cut(s) 540, 1625
MslI CAYNNNNRTG 2 cut(s) 1782, 3045
Msp20I TGGCCA 2 cut(s) 540, 1625
MspA1I CMGCKG 1 cut(s) 2582
MspCI CTTAAG 1 cut(s) 1171
MspI CCGG 5 cut(s) 1647, 1819, 2789, 3093, 3103
MspR9I CCNGG 9 cut(s) 1372, 1627, 1820, 2328, 2410, 2546, 2648, 2773, 2968
Mva1269I GAATGC 3 cut(s) 348, 804, 1252
MvaI CCWGG 8 cut(s) 1372, 1627, 2328, 2410, 2546, 2648, 2773, 2968
NaeI GCCGGC 1 cut(s) 3094
NciI CCSGG 1 cut(s) 1820
NgoMIV GCCGGC 1 cut(s) 3092
NlaIV GGNNCC 3 cut(s) 1299, 3052, 3066
NmeAIII GCCGAG 3 cut(s) 2475, 2557, 2602
NmuCI GTSAC 3 cut(s) 1106, 2237, 2590
NspI RCATGY 2 cut(s) 2512, 2936
NspV TTCGAA 1 cut(s) 432
PaeI GCATGC 2 cut(s) 2512, 2936
PagI TCATGA 3 cut(s) 1549, 2683, 3007
PaqCI CACCTGC 1 cut(s) 3082
PceI AGGCCT 1 cut(s) 2615
PciSI GCTCTTC 1 cut(s) 3126
PctI GAATGC 3 cut(s) 348, 804, 1252
PdiI GCCGGC 1 cut(s) 3094
PflFI GACNNNGTC 1 cut(s) 1104
PflMI CCANNNNNTGG 2 cut(s) 1150, 2551
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
Ppu21I YACGTR 1 cut(s) 1696
PpuMI RGGWCCY 1 cut(s) 1298
PshBI ATTAAT 1 cut(s) 1938
PsiI TTATAA 1 cut(s) 981
Psp124BI GAGCTC 2 cut(s) 1049, 3004
Psp5II RGGWCCY 1 cut(s) 1298
Psp6I CCWGG 8 cut(s) 1370, 1625, 2326, 2408, 2544, 2646, 2771, 2966
PspEI GGTNACC 1 cut(s) 1793
PspGI CCWGG 8 cut(s) 1370, 1625, 2326, 2408, 2544, 2646, 2771, 2966
PspN4I GGNNCC 3 cut(s) 1299, 3052, 3066
PspPI GGNCC 2 cut(s) 1298, 2947
PspPPI RGGWCCY 1 cut(s) 1298
PsrI GAACNNNNNNTAC 2 cut(s) 708, 740
PstI CTGCAG 1 cut(s) 754
PsuI RGATCY 3 cut(s) 116, 596, 1607
PsyI GACNNNGTC 1 cut(s) 1104
RsaI GTAC 6 cut(s) 134, 256, 593, 726, 1183, 2110
RsaNI GTAC 6 cut(s) 133, 255, 592, 725, 1182, 2109
RseI CAYNNNNRTG 2 cut(s) 1782, 3045
SacI GAGCTC 2 cut(s) 1049, 3004
SapI GCTCTTC 1 cut(s) 3126
Sau96I GGNCC 2 cut(s) 1298, 2947
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 9 cut(s) 1372, 1627, 1820, 2328, 2410, 2546, 2648, 2773, 2968
SduI GDGCHC 4 cut(s) 1049, 2736, 2820, 3004
SfaNI GCATC 1 cut(s) 2754
SfcI CTRYAG 2 cut(s) 750, 1574
SfuI TTCGAA 1 cut(s) 432
SinI GGWCC 2 cut(s) 1298, 2947
SmiMI CAYNNNNRTG 2 cut(s) 1782, 3045
SmlI CTYRAG 8 cut(s) 830, 839, 1171, 1271, 1423, 2142, 2757, 3024
SmoI CTYRAG 8 cut(s) 830, 839, 1171, 1271, 1423, 2142, 2757, 3024
SnaBI TACGTA 1 cut(s) 1696
SphI GCATGC 2 cut(s) 2512, 2936
SseBI AGGCCT 1 cut(s) 2615
SsiI CCGC 9 cut(s) 1016, 1197, 2577, 2580, 2723, 3029, 3044, 3108, 3112
SspI AATATT 3 cut(s) 182, 271, 1526
SspMI CTAG 6 cut(s) 54, 1230, 1845, 2130, 2807, 3136
SstI GAGCTC 2 cut(s) 1049, 3004
StuI AGGCCT 1 cut(s) 2615
StyD4I CCNGG 9 cut(s) 1370, 1625, 1818, 2326, 2408, 2544, 2646, 2771, 2966
TaaI ACNGT 8 cut(s) 557, 1137, 1541, 1924, 1948, 2243, 2387, 2596
TaiI ACGT 5 cut(s) 79, 1435, 1600, 1698, 2692
TaqI TCGA 7 cut(s) 432, 632, 1029, 1531, 1717, 2656, 3127
TaqII GACCGA 1 cut(s) 2964
TatI WGTACW 1 cut(s) 132
TauI GCSGC 2 cut(s) 2579, 2582
TscAI CASTG 3 cut(s) 556, 2599, 2820
TseFI GTSAC 3 cut(s) 1106, 2237, 2590
TseI GCWGC 8 cut(s) 749, 2567, 2570, 2573, 2582, 2919, 2963, 2990
Tsp45I GTSAC 3 cut(s) 1106, 2237, 2590
TspGWI ACGGA 1 cut(s) 3008
TspRI CASTG 3 cut(s) 556, 2599, 2820
Tth111I GACNNNGTC 1 cut(s) 1104
Van91I CCANNNNNTGG 2 cut(s) 1150, 2551
Vha464I CTTAAG 1 cut(s) 1171
VneI GTGCAC 1 cut(s) 2816
VpaK11BI GGWCC 2 cut(s) 1298, 2947
VspI ATTAAT 1 cut(s) 1938
XagI CCTNNNNNAGG 1 cut(s) 2609
XapI RAATTY 9 cut(s) 108, 338, 364, 427, 959, 1114, 1507, 1514, 2050
XceI RCATGY 2 cut(s) 2512, 2936
XcmI CCANNNNNNNNNTGG 1 cut(s) 1147
XmiI GTMKAC 1 cut(s) 2382
XspI CTAG 6 cut(s) 54, 1230, 1845, 2130, 2807, 3136
ZraI GACGTC 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.