Rmu_ssc0000118.1_g000016

spliceosomal complex assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000118.1
OG0000703
Physical Location & Seq
Forward (+)
57786 .. 62288
4503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000118.1_g000016.1.cds

Sequence Viewer

Length: 975 bp
atgatcgtccttagtcttgatcctgaaaaggatcatcttcgtctgaaggataatgacgaagatgtgctagaggcagaagtgccttacttgagtataatagaagcattattagagaaaatggatttggatccatcacacaccagaaacgccgccaacgctggcctgcgttctctatccccatcccaaaatgatataagtgttttggaaggttttgctgatgttggtcatcagggggagacgcagacatcaggggagatgtctacatgttcacctccaaacgtgaagggaatgccaaagggggagattgtaggtataaggtttacacttgatgtgttggcatccctatacaaagcactgaagaagcctacaaagttcttcaaaattgatctcggagaatttcagtcaactttgagcgatagtgaggaagattctcaaactaagaatgaagaagagaacattgcattcacctcctcacttcatcatgaggcatctgatgattccaacaatgaaggcgagatttttgatgtatctgatgatgagactaatgataagtgcagggaattgtataaagcttcaagagtgatgctcaaaagaaatctcaagttggagaacgagattgagcaagataagatgaattcccaaacttgggaggtggagcgtactgagttgattgacatgattaaaacccttcaagttgaagttaaagctcaatcaaatttgaatgtctcactaacctctgaaactgagaatttgcaagttaagcttaaagtgtctcaagaaaagttcaacaaattctcaataaggtcagatttggtttccaaaatgattggaattggaaaaccacacaccaacaagaaagggttggggtatgaaggtgtaaaaatcgatcgttctgattgtgtgaaaggtgtgagctcaagtgtgcactcaagctcatcaaatgaccaagaaattcaccccccatctctttcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve zf-CCHC

Protein Analysis

324

Amino Acids

36.11

Weight (kDa)

4.86

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 260
AciI CCGC 1 cut(s) 150
AclWI GGATC 4 cut(s) 14, 39, 122, 135
AcsI RAATTY 6 cut(s) 395, 634, 715, 748, 791, 951
AcuI CTGAAG 2 cut(s) 65, 377
AfaI GTAC 1 cut(s) 661
AfiI CCNNNNNNNGG 2 cut(s) 645, 646
AflIII ACRYGT 1 cut(s) 263
AgsI TTSAA 6 cut(s) 379, 576, 692, 698, 721, 787
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 5 cut(s) 572, 707, 763, 915, 933
AluI AGCT 5 cut(s) 572, 707, 763, 915, 933
Alw21I GWGCWC 2 cut(s) 917, 927
Alw26I GTCTC 4 cut(s) 230, 533, 730, 777
Alw44I GTGCAC 1 cut(s) 923
AlwI GGATC 4 cut(s) 14, 39, 122, 135
AoxI GGCC 1 cut(s) 160
ApaLI GTGCAC 1 cut(s) 923
ApoI RAATTY 6 cut(s) 395, 634, 715, 748, 791, 951
AsuHPI GGTGA 3 cut(s) 261, 457, 947
BaeGI GKGCMC 1 cut(s) 927
BamHI GGATCC 1 cut(s) 127
BanII GRGCYC 1 cut(s) 917
Bbv12I GWGCWC 2 cut(s) 917, 927
BccI CCATC 3 cut(s) 139, 187, 970
BcoDI GTCTC 4 cut(s) 230, 533, 730, 777
BfaI CTAG 1 cut(s) 68
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
BmiI GGNNCC 1 cut(s) 129
BmsI GCATC 3 cut(s) 347, 497, 573
BplI GAGNNNNNCTC 2 cut(s) 570, 602
BpuEI CTTGAG 5 cut(s) 109, 584, 759, 901, 913
Bsa29I ATCGAT 1 cut(s) 885
BsaBI GATNNNNATC 1 cut(s) 126
Bsc4I CCNNNNNNNGG 2 cut(s) 645, 646
Bse3DI GCAATG 1 cut(s) 456
Bse8I GATNNNNATC 1 cut(s) 126
BseCI ATCGAT 1 cut(s) 885
BseGI GGATG 2 cut(s) 179, 338
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 2 cut(s) 645, 646
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 2 cut(s) 654, 735
BseRI GAGGAG 1 cut(s) 460
BseSI GKGCMC 1 cut(s) 927
BsgI GTGCAG 1 cut(s) 574
Bsh1285I CGRYCG 1 cut(s) 889
BshFI GGCC 1 cut(s) 162
BshVI ATCGAT 1 cut(s) 885
BsiEI CGRYCG 1 cut(s) 889
BsiHKAI GWGCWC 2 cut(s) 917, 927
BslI CCNNNNNNNGG 2 cut(s) 645, 646
BsmAI GTCTC 4 cut(s) 230, 533, 730, 777
BsmBI CGTCTC 1 cut(s) 230
BsmI GAATGC 2 cut(s) 294, 461
BsnI GGCC 1 cut(s) 162
Bsp1286I GDGCHC 2 cut(s) 917, 927
Bsp143I GATC 6 cut(s) 3, 19, 31, 127, 385, 886
BspACI CCGC 1 cut(s) 150
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 2 cut(s) 655, 736
BspDI ATCGAT 1 cut(s) 885
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 1 cut(s) 129
BspPI GGATC 4 cut(s) 14, 39, 122, 135
BsrDI GCAATG 1 cut(s) 456
BssMI GATC 6 cut(s) 3, 19, 31, 127, 385, 886
Bst6I CTCTTC 1 cut(s) 444
BstC8I GCNNGC 2 cut(s) 160, 164
BstDEI CTNAG 4 cut(s) 11, 438, 663, 744
BstF5I GGATG 2 cut(s) 179, 338
BstKTI GATC 6 cut(s) 6, 22, 34, 130, 388, 889
BstMAI GTCTC 4 cut(s) 230, 533, 730, 777
BstMBI GATC 6 cut(s) 3, 19, 31, 127, 385, 886
BstMCI CGRYCG 1 cut(s) 889
BstMWI GCNNNNNNNGC 2 cut(s) 155, 760
BstNSI RCATGY 1 cut(s) 267
BstSLI GKGCMC 1 cut(s) 927
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
Bsu15I ATCGAT 1 cut(s) 885
BsuRI GGCC 1 cut(s) 162
BsuTUI ATCGAT 1 cut(s) 885
BtsCI GGATG 2 cut(s) 179, 338
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 2 cut(s) 160, 164
CciI TCATGA 1 cut(s) 481
ClaI ATCGAT 1 cut(s) 885
CseI GACGC 1 cut(s) 247
Csp6I GTAC 1 cut(s) 660
CviAII CATG 3 cut(s) 264, 482, 676
CviJI RGCY 7 cut(s) 162, 364, 572, 707, 763, 915, 933
CviKI_1 RGCY 7 cut(s) 162, 364, 572, 707, 763, 915, 933
CviQI GTAC 1 cut(s) 660
DdeI CTNAG 4 cut(s) 11, 438, 663, 744
DpnI GATC 6 cut(s) 5, 21, 33, 129, 387, 888
DpnII GATC 6 cut(s) 3, 19, 31, 127, 385, 886
Eam1104I CTCTTC 1 cut(s) 444
EarI CTCTTC 1 cut(s) 444
Ecl136II GAGCTC 1 cut(s) 915
Eco24I GRGCYC 1 cut(s) 917
Eco53kI GAGCTC 1 cut(s) 915
Eco57I CTGAAG 2 cut(s) 65, 377
EcoICRI GAGCTC 1 cut(s) 915
EcoRI GAATTC 1 cut(s) 634
EcoT38I GRGCYC 1 cut(s) 917
Esp3I CGTCTC 1 cut(s) 230
FaeI CATG 3 cut(s) 267, 485, 679
FaiI YATR 9 cut(s) 95, 194, 265, 314, 346, 483, 567, 677, 870
FalI AAGNNNNNCTT 2 cut(s) 747, 779
FatI CATG 3 cut(s) 263, 481, 675
FblI GTMKAC 1 cut(s) 260
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 2 cut(s) 166, 325
FriOI GRGCYC 1 cut(s) 917
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 1 cut(s) 68
GluI GCNGC 1 cut(s) 150
HaeIII GGCC 1 cut(s) 162
HgaI GACGC 1 cut(s) 247
Hin1II CATG 3 cut(s) 267, 485, 679
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HindIII AAGCTT 2 cut(s) 570, 761
HinfI GANTC 2 cut(s) 428, 497
HphI GGTGA 3 cut(s) 261, 457, 947
Hpy166II GTNNAC 5 cut(s) 261, 269, 321, 405, 925
Hpy188I TCNGA 7 cut(s) 45, 392, 493, 532, 739, 808, 895
Hpy188III TCNNGA 5 cut(s) 17, 23, 482, 576, 776
Hpy8I GTNNAC 5 cut(s) 261, 269, 321, 405, 925
HpyAV CCTTC 6 cut(s) 40, 200, 277, 503, 698, 866
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 4 cut(s) 461, 555, 754, 925
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 760
HpyF3I CTNAG 4 cut(s) 11, 438, 663, 744
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 3 cut(s) 267, 485, 679
Kzo9I GATC 6 cut(s) 3, 19, 31, 127, 385, 886
LmnI GCTCC 1 cut(s) 655
LpnPI CCDG 7 cut(s) 36, 144, 154, 176, 215, 234, 541
LweI GCATC 3 cut(s) 347, 497, 573
MaeI CTAG 1 cut(s) 68
MaeII ACGT 1 cut(s) 279
MalI GATC 6 cut(s) 5, 21, 33, 129, 387, 888
MboI GATC 6 cut(s) 3, 19, 31, 127, 385, 886
MboII GAAGA 7 cut(s) 29, 71, 367, 370, 437, 458, 461
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 2 cut(s) 917, 927
MluCI AATT 9 cut(s) 381, 395, 560, 634, 715, 748, 791, 831, 951
MmeI TCCRAC 2 cut(s) 525, 585
MnlI CCTC 8 cut(s) 64, 282, 415, 478, 478, 481, 643, 745
MseI TTAA 5 cut(s) 681, 702, 759, 765, 973
Mva1269I GAATGC 2 cut(s) 294, 461
MwoI GCNNNNNNNGC 2 cut(s) 155, 760
NdeII GATC 6 cut(s) 3, 19, 31, 127, 385, 886
NlaIII CATG 3 cut(s) 267, 485, 679
NlaIV GGNNCC 1 cut(s) 129
NspI RCATGY 1 cut(s) 267
PagI TCATGA 1 cut(s) 481
PciI ACATGT 1 cut(s) 263
PctI GAATGC 2 cut(s) 294, 461
PfeI GAWTC 2 cut(s) 428, 497
PkrI GCNGC 1 cut(s) 151
Ple19I CGATCG 1 cut(s) 889
PscI ACATGT 1 cut(s) 263
Psp124BI GAGCTC 1 cut(s) 917
PspN4I GGNNCC 1 cut(s) 129
PsuI RGATCY 1 cut(s) 127
PvuI CGATCG 1 cut(s) 889
RsaI GTAC 1 cut(s) 661
RsaNI GTAC 1 cut(s) 660
SacI GAGCTC 1 cut(s) 917
SaqAI TTAA 5 cut(s) 681, 702, 759, 765, 973
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 6 cut(s) 3, 19, 31, 127, 385, 886
SduI GDGCHC 2 cut(s) 917, 927
SfaNI GCATC 3 cut(s) 347, 497, 573
SmlI CTYRAG 5 cut(s) 88, 599, 774, 916, 928
SmoI CTYRAG 5 cut(s) 88, 599, 774, 916, 928
Sse9I AATT 9 cut(s) 381, 395, 560, 634, 715, 748, 791, 831, 951
SsiI CCGC 1 cut(s) 150
SspMI CTAG 1 cut(s) 68
SstI GAGCTC 1 cut(s) 917
TaiI ACGT 1 cut(s) 282
TaqI TCGA 1 cut(s) 885
TasI AATT 9 cut(s) 381, 395, 560, 634, 715, 748, 791, 831, 951
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 2 cut(s) 428, 497
Tru1I TTAA 5 cut(s) 681, 702, 759, 765, 973
Tru9I TTAA 5 cut(s) 681, 702, 759, 765, 973
TscAI CASTG 1 cut(s) 360
TspDTI ATGAA 5 cut(s) 459, 467, 522, 647, 885
TspRI CASTG 1 cut(s) 360
VneI GTGCAC 1 cut(s) 923
XapI RAATTY 6 cut(s) 395, 634, 715, 748, 791, 951
XceI RCATGY 1 cut(s) 267
XmiI GTMKAC 1 cut(s) 260
XspI CTAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.