FvH4_2g11420

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000440
Physical Location & Seq
Reverse (-)
9962509 .. 9966537
4029 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g11420.t1

Sequence Viewer

Length: 1476 bp
ATGGGTTTACTGGGTGTTCTTGGATTTGTGCTCCAATGCTTTGATGTTCTTGCATGGCCTCTCTTTGCTCTGCTCTATCCTCTGTATTCTTCCATAAGAGCAATCGAGACAAATTCCATTTCAGATACTCACAAGTTAAATACATATTGGGTCGTTTTCTCATTGATCTTGCTCTTTGAACATGCTTTTATGAAGCTGCTTGAATGCTGCTCGCTCTGGCCATACATGAGGCTGCTGTTTGTCTTCTTTTTGGTGATACCACATTTTGATGGTGCCTTGTATGTCTTTAAACACCTTATCTGTCCATGTCTCTCCATGGATCCTCAAATTATATTTAGCTTGTTTAAAAAGCGGAAGGAGTCCTTGGTTAGAGAAAACTTTCTATCTGAGGTGGAAAGATATGTAAAGGAGAATGGATATGAAGGTTTGGAAAAGTCTATCTCTTGCAAGTCAATATCCATAAAGCCAAATGGTGGACAGCATGGTATGCAGGTAATGGTGAAAAAGAAGGAAACAACTGATAACCAAGTTAGTGAGGCAGAGCCTATTCTCACTGTCAATAAAGATAGATCTTCTGCTATGCAAATGAAAGAAAAAACCATTGAGGTTGCAGCAGACAGAAGAATTCTGGAGACTCCGCCTTCAAAGAAAGTCCAAAAGGAGTTGACTGCAGTCAGTGTGCTAGCAACTCAAACTGATGCAAAAAACATTGAGGCTGCAGCAGAAAGCGGAATTCTAAAGACTCCGCCTCCAAAGAAAGTCCAAGGGGAGTTGACCGCAGTCTGTCCGCTGCCAATTCAAACTGATGCAACTTTTAATTTCCCTCTCGGTGGTAATAAACACAATGCTACATATGAGGCAAAGCCTAATCTCACTCTGACTGAAAACCATACTGTTCCAGCTGTGAAAGTTAATGAAAAGCCGATCCAAGTTGGAACAGGTATGGAACATCCCCAGTCACCTAAACAAGTCCAGAAAGAGTGGACTTGTGCTCTCTGTCAGGTAACCACTAAATGTGAAACAACTTTGAATTCCCACCTGCAAGGGAGGAAACACAAGGCTTCATATGATGCACTGAAAGCAAGGAACCAGGCATTTGTGCCCACGATTTCCCCAGCTTCAACTGCAAAGACATCCCATCATCCAAATAAGGAGCCAGGAAAGAGCATTCCAAGCAGTGGAGTGAAACAAAAAGTTACTTCAAATGAACACATGCAAGCCCAGAAAAAAATTAGTCCAATTACAAAGCTCGATAAACTGAAAGAGGTGCTAGTAGATGGCGTATCCAGCAATGGCCTGAAGGGGAAAAGTGCTATACATCTTGAGAAGGTACAAAGTCAACAACCGAAGCGGAATTCCCCTTTGTGGTGCACAGTTTGTAGCATACGCTGCCCTGGTAAGATTGACATGATGTGTCATCTCAAGGGAAGGAAACACAAGGAGAATGAACAACGACAAGAGAACCTACCACAATAG

Protein Analysis

492

Amino Acids

54.92

Weight (kDa)

9.25

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 27 - 103 2.7e-18 TB2/DP1, HVA22 family
zf-met PF12874 328 - 352 4.8e-09 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000440)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20313 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_5g25851
prunus_persica Prupe.8G072900_v2.0.a1 Prupe.8G072900_v2.0.a1 Prupe.8G072900_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1
pyrus_communis pycom01g01210 pycom02g12000 pycom10g21410 pycom11g11060 pycom13g24280
rosa_chinensis RchiOBHm_Chr3g0461871 RchiOBHm_Chr4g0427221 RchiOBHm_Chr4g0427231 RchiOBHm_Chr6g0270081 RchiOBHm_Chr6g0270121 RchiOBHm_Chr6g0270151 RchiOBHm_Chr6g0270161 RchiOBHm_Chr6g0270171 RchiOBHm_Chr6g0270181 RchiOBHm_Chr6g0270191 RchiOBHm_Chr7g0229301
rosa_laevigata RLG00000013809 RLG00000013810 RLG00000013812 RLG00000013814 RLG00000013817 RLG00000013818
rosa_multiflora Rmu_sc0000918.1_g000004 Rmu_sc0000918.1_g000019 Rmu_sc0000918.1_g000027 Rmu_sc0002817.1_g000013 Rmu_sc0004366.1_g000009 Rmu_sc0004376.1_g000011 Rmu_sc0004376.1_g000014 Rmu_sc0004376.1_g000021 Rmu_sc0006909.1_g000002 Rmu_sc0011356.1_g000003 Rmu_sc0011369.1_g000005 Rmu_sc0017552.1_g000001
rosa_roxburghii Rroxscaffold_5G00354200 Rroxscaffold_7G00198080 Rroxscaffold_7G00198090 Rroxscaffold_7G00198100 Rroxscaffold_7G00198130 Rroxscaffold_7G00198170
rosa_rugosa Rorug06G0052400 Rorug06G0052500 Rorug06G0053000
rosa_samantha Rh6AG171300 Rh6AG171400 Rh6AG171800 Rh6AG171900 Rh6AG172000 Rh6AG172100 Rh6AG369700 Rh6BG175800 Rh6BG175900 Rh6BG176200 Rh6BG176300 Rh6CG170900 Rh6CG171100 Rh6CG171400 Rh6CG171500 Rh6CG171700 Rh6CG171900 Rh6CG172000 Rh6CG172100
rosa_wichuraiana Rw6G014730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1047
Acc36I ACCTGC 2 cut(s) 481, 1047
AccB1I GGYRCC 1 cut(s) 272
AccB7I CCANNNNNTGG 2 cut(s) 473, 1178
AciI CCGC 7 cut(s) 352, 638, 729, 746, 777, 788, 1351
AclWI GGATC 3 cut(s) 314, 327, 919
AcoI YGGCCR 1 cut(s) 218
AcsI RAATTY 5 cut(s) 112, 624, 732, 1030, 1354
AcuI CTGAAG 1 cut(s) 1319
AfaI GTAC 1 cut(s) 1332
AfiI CCNNNNNNNGG 4 cut(s) 473, 830, 1178, 1365
AgsI TTSAA 7 cut(s) 179, 203, 645, 800, 1030, 1122, 1203
AjnI CCWGG 3 cut(s) 1089, 1156, 1393
AjuI GAANNNNNNNTTGG 2 cut(s) 347, 379
AluBI AGCT 5 cut(s) 196, 339, 902, 1118, 1249
AluI AGCT 5 cut(s) 196, 339, 902, 1118, 1249
Alw21I GWGCWC 3 cut(s) 33, 994, 1373
Alw26I GTCTC 3 cut(s) 101, 314, 626
Alw44I GTGCAC 1 cut(s) 1369
AlwI GGATC 3 cut(s) 314, 327, 919
AoxI GGCC 3 cut(s) 56, 218, 1294
ApaLI GTGCAC 1 cut(s) 1369
ApeKI GCWGC 8 cut(s) 196, 207, 232, 611, 716, 719, 790, 1389
ApoI RAATTY 5 cut(s) 112, 624, 732, 1030, 1354
Asp700I GAANNNNTTC 1 cut(s) 378
AsuHPI GGTGA 3 cut(s) 265, 511, 951
AsuNHI GCTAGC 1 cut(s) 682
BaeGI GKGCMC 2 cut(s) 1104, 1373
BalI TGGCCA 1 cut(s) 220
BamHI GGATCC 1 cut(s) 319
BanI GGYRCC 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 235
Bbv12I GWGCWC 3 cut(s) 33, 994, 1373
BbvI GCAGC 8 cut(s) 183, 194, 219, 623, 703, 731, 777, 1376
BccI CCATC 3 cut(s) 263, 1146, 1271
BciT130I CCWGG 3 cut(s) 1091, 1158, 1395
BciVI GTATCC 1 cut(s) 1294
BcoDI GTCTC 3 cut(s) 101, 314, 626
BfaI CTAG 2 cut(s) 683, 1271
BfmI CTRYAG 2 cut(s) 669, 717
BfuAI ACCTGC 2 cut(s) 481, 1047
BfuI GTATCC 1 cut(s) 1294
BglII AGATCT 1 cut(s) 569
BisI GCNGC 8 cut(s) 197, 208, 233, 612, 717, 720, 791, 1390
BlsI GCNGC 8 cut(s) 198, 209, 234, 613, 718, 721, 792, 1391
Bme1390I CCNGG 3 cut(s) 1091, 1158, 1395
BmiI GGNNCC 4 cut(s) 274, 321, 1088, 1155
BmrFI CCNGG 3 cut(s) 1091, 1158, 1395
BmrI ACTGGG 2 cut(s) 20, 949
BmsI GCATC 3 cut(s) 688, 796, 1060
BmtI GCTAGC 1 cut(s) 686
BmuI ACTGGG 2 cut(s) 20, 949
BoxI GACNNNNGTC 2 cut(s) 671, 779
BpiI GAAGAC 1 cut(s) 235
BpmI CTGGAG 1 cut(s) 650
BpuEI CTTGAG 2 cut(s) 1343, 1406
BsaJI CCNNGG 4 cut(s) 315, 363, 763, 1393
BsaXI ACNNNNNCTCC 2 cut(s) 733, 763
Bsc4I CCNNNNNNNGG 4 cut(s) 473, 830, 1178, 1365
Bse1I ACTGG 2 cut(s) 15, 955
Bse3DI GCAATG 1 cut(s) 1297
BseBI CCWGG 3 cut(s) 1091, 1158, 1395
BseDI CCNNGG 4 cut(s) 315, 363, 763, 1393
BseGI GGATG 3 cut(s) 949, 1133, 1141
BseLI CCNNNNNNNGG 4 cut(s) 473, 830, 1178, 1365
BseMI GCAATG 1 cut(s) 1297
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 2 cut(s) 15, 955
BseSI GKGCMC 2 cut(s) 1104, 1373
BseXI GCAGC 8 cut(s) 183, 194, 219, 623, 703, 731, 777, 1376
BseYI CCCAGC 1 cut(s) 1114
BshFI GGCC 3 cut(s) 58, 220, 1296
BshNI GGYRCC 1 cut(s) 272
BsiHKAI GWGCWC 3 cut(s) 33, 994, 1373
BslI CCNNNNNNNGG 4 cut(s) 473, 830, 1178, 1365
BsmAI GTCTC 3 cut(s) 101, 314, 626
BsmI GAATGC 2 cut(s) 209, 1167
BsnI GGCC 3 cut(s) 58, 220, 1296
Bsp1286I GDGCHC 4 cut(s) 33, 994, 1104, 1373
Bsp143I GATC 4 cut(s) 165, 319, 569, 924
Bsp19I CCATGG 1 cut(s) 315
BspACI CCGC 7 cut(s) 352, 638, 729, 746, 777, 788, 1351
BspANI GGCC 3 cut(s) 58, 220, 1296
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 4 cut(s) 274, 321, 1088, 1155
BspMAI CTGCAG 2 cut(s) 673, 721
BspMI ACCTGC 2 cut(s) 481, 1047
BspOI GCTAGC 1 cut(s) 686
BspPI GGATC 3 cut(s) 314, 327, 919
BspT107I GGYRCC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 1297
BsrI ACTGG 2 cut(s) 15, 955
BssECI CCNNGG 4 cut(s) 315, 363, 763, 1393
BssMI GATC 4 cut(s) 165, 319, 569, 924
BssT1I CCWWGG 3 cut(s) 315, 363, 763
Bst2UI CCWGG 3 cut(s) 1091, 1158, 1395
Bst4CI ACNGT 3 cut(s) 556, 895, 1375
BstAPI GCANNNNNTGC 2 cut(s) 487, 1389
BstC8I GCNNGC 3 cut(s) 212, 684, 1218
BstDEI CTNAG 1 cut(s) 387
BstDSI CCRYGG 1 cut(s) 315
BstEII GGTNACC 1 cut(s) 1003
BstF5I GGATG 3 cut(s) 949, 1133, 1141
BstKTI GATC 4 cut(s) 168, 322, 572, 927
BstMAI GTCTC 3 cut(s) 101, 314, 626
BstMBI GATC 4 cut(s) 165, 319, 569, 924
BstMWI GCNNNNNNNGC 6 cut(s) 487, 1079, 1124, 1173, 1287, 1389
BstNI CCWGG 3 cut(s) 1091, 1158, 1395
BstNSI RCATGY 2 cut(s) 185, 1216
BstPAI GACNNNNGTC 2 cut(s) 671, 779
BstPI GGTNACC 1 cut(s) 1003
BstSCI CCNGG 3 cut(s) 1089, 1156, 1393
BstSFI CTRYAG 2 cut(s) 669, 717
BstSLI GKGCMC 2 cut(s) 1104, 1373
BstV1I GCAGC 8 cut(s) 183, 194, 219, 623, 703, 731, 777, 1376
BstV2I GAAGAC 1 cut(s) 235
BstX2I RGATCY 2 cut(s) 319, 569
BstYI RGATCY 2 cut(s) 319, 569
BsuI GTATCC 1 cut(s) 1294
BsuRI GGCC 3 cut(s) 58, 220, 1296
BtgI CCRYGG 1 cut(s) 315
BtsCI GGATG 3 cut(s) 949, 1133, 1141
BtsI GCAGTG 1 cut(s) 1183
BtsIMutI CAGTG 4 cut(s) 552, 682, 1073, 1183
BveI ACCTGC 2 cut(s) 481, 1047
Cac8I GCNNGC 3 cut(s) 212, 684, 1218
Csp6I GTAC 1 cut(s) 1331
CviAII CATG 8 cut(s) 54, 182, 226, 306, 316, 482, 1213, 1408
CviQI GTAC 1 cut(s) 1331
DdeI CTNAG 1 cut(s) 387
DpnI GATC 4 cut(s) 167, 321, 571, 926
DpnII GATC 4 cut(s) 165, 319, 569, 924
DraI TTTAAA 2 cut(s) 289, 346
EaeI YGGCCR 1 cut(s) 218
EciI GGCGGA 2 cut(s) 627, 735
Eco130I CCWWGG 3 cut(s) 315, 363, 763
Eco57I CTGAAG 1 cut(s) 1319
Eco91I GGTNACC 1 cut(s) 1003
EcoO65I GGTNACC 1 cut(s) 1003
EcoRI GAATTC 4 cut(s) 624, 732, 1030, 1354
EcoRII CCWGG 3 cut(s) 1089, 1156, 1393
EcoT14I CCWWGG 3 cut(s) 315, 363, 763
ErhI CCWWGG 3 cut(s) 315, 363, 763
FaeI CATG 8 cut(s) 57, 185, 229, 309, 319, 485, 1216, 1411
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FatI CATG 8 cut(s) 53, 181, 225, 305, 315, 481, 1212, 1407
FauNDI CATATG 2 cut(s) 853, 1066
Fnu4HI GCNGC 8 cut(s) 197, 208, 233, 612, 717, 720, 791, 1390
FokI GGATG 3 cut(s) 936, 1120, 1128
Fsp4HI GCNGC 8 cut(s) 197, 208, 233, 612, 717, 720, 791, 1390
FspBI CTAG 2 cut(s) 683, 1271
GluI GCNGC 8 cut(s) 197, 208, 233, 612, 717, 720, 791, 1390
GsaI CCCAGC 1 cut(s) 1118
GsuI CTGGAG 1 cut(s) 650
HaeIII GGCC 3 cut(s) 58, 220, 1296
Hin1II CATG 8 cut(s) 57, 185, 229, 309, 319, 485, 1216, 1411
HincII GTYRAC 3 cut(s) 666, 774, 1340
HindII GTYRAC 3 cut(s) 666, 774, 1340
HinfI GANTC 3 cut(s) 359, 634, 742
HphI GGTGA 3 cut(s) 265, 511, 951
Hpy166II GTNNAC 7 cut(s) 8, 476, 666, 774, 984, 1340, 1371
Hpy188I TCNGA 3 cut(s) 124, 388, 879
Hpy188III TCNNGA 4 cut(s) 106, 629, 973, 1322
Hpy8I GTNNAC 7 cut(s) 8, 476, 666, 774, 984, 1340, 1371
HpyAV CCTTC 7 cut(s) 349, 416, 502, 651, 1294, 1321, 1422
HpyCH4III ACNGT 3 cut(s) 556, 895, 1375
HpyF10VI GCNNNNNNNGC 6 cut(s) 487, 1079, 1124, 1173, 1287, 1389
HpyF3I CTNAG 1 cut(s) 387
Hsp92II CATG 8 cut(s) 57, 185, 229, 309, 319, 485, 1216, 1411
Kzo9I GATC 4 cut(s) 165, 319, 569, 924
LmnI GCTCC 2 cut(s) 36, 1153
Lsp1109I GCAGC 8 cut(s) 183, 194, 219, 623, 703, 731, 777, 1376
LweI GCATC 3 cut(s) 688, 796, 1060
MaeI CTAG 2 cut(s) 683, 1271
MaeIII GTNAC 3 cut(s) 957, 1003, 1195
MalI GATC 4 cut(s) 167, 321, 571, 926
MboI GATC 4 cut(s) 165, 319, 569, 924
MboII GAAGA 4 cut(s) 81, 235, 564, 633
MflI RGATCY 2 cut(s) 319, 569
MhlI GDGCHC 4 cut(s) 33, 994, 1104, 1373
MlsI TGGCCA 1 cut(s) 220
MluNI TGGCCA 1 cut(s) 220
MlyI GAGTC 3 cut(s) 368, 628, 736
MmeI TCCRAC 1 cut(s) 913
Mox20I TGGCCA 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 378
MscI TGGCCA 1 cut(s) 220
MseI TTAA 5 cut(s) 137, 288, 345, 816, 912
MslI CAYNNNNRTG 1 cut(s) 267
Msp20I TGGCCA 1 cut(s) 220
MspA1I CMGCKG 2 cut(s) 790, 902
MspR9I CCNGG 3 cut(s) 1091, 1158, 1395
Mva1269I GAATGC 2 cut(s) 209, 1167
MvaI CCWGG 3 cut(s) 1091, 1158, 1395
MwoI GCNNNNNNNGC 6 cut(s) 487, 1079, 1124, 1173, 1287, 1389
NcoI CCATGG 1 cut(s) 315
NdeI CATATG 2 cut(s) 853, 1066
NdeII GATC 4 cut(s) 165, 319, 569, 924
NheI GCTAGC 1 cut(s) 682
NlaIII CATG 8 cut(s) 57, 185, 229, 309, 319, 485, 1216, 1411
NlaIV GGNNCC 4 cut(s) 274, 321, 1088, 1155
NmuCI GTSAC 1 cut(s) 957
NspI RCATGY 2 cut(s) 185, 1216
PaqCI CACCTGC 1 cut(s) 1047
PctI GAATGC 2 cut(s) 209, 1167
PdmI GAANNNNTTC 1 cut(s) 378
PflMI CCANNNNNTGG 2 cut(s) 473, 1178
PkrI GCNGC 8 cut(s) 198, 209, 234, 613, 718, 721, 792, 1391
PleI GAGTC 3 cut(s) 367, 628, 736
PpsI GAGTC 3 cut(s) 367, 628, 736
PshAI GACNNNNGTC 2 cut(s) 671, 779
Psp6I CCWGG 3 cut(s) 1089, 1156, 1393
PspEI GGTNACC 1 cut(s) 1003
PspFI CCCAGC 1 cut(s) 1114
PspGI CCWGG 3 cut(s) 1089, 1156, 1393
PspN4I GGNNCC 4 cut(s) 274, 321, 1088, 1155
PstI CTGCAG 2 cut(s) 673, 721
PsuI RGATCY 2 cut(s) 319, 569
PvuII CAGCTG 1 cut(s) 902
RsaI GTAC 1 cut(s) 1332
RsaNI GTAC 1 cut(s) 1331
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 5 cut(s) 137, 288, 345, 816, 912
SatI GCNGC 8 cut(s) 197, 208, 233, 612, 717, 720, 791, 1390
Sau3AI GATC 4 cut(s) 165, 319, 569, 924
SchI GAGTC 3 cut(s) 368, 628, 736
ScrFI CCNGG 3 cut(s) 1091, 1158, 1395
SduI GDGCHC 4 cut(s) 33, 994, 1104, 1373
SfaNI GCATC 3 cut(s) 688, 796, 1060
SfcI CTRYAG 2 cut(s) 669, 717
SmiMI CAYNNNNRTG 1 cut(s) 267
SmlI CTYRAG 2 cut(s) 1322, 1421
SmoI CTYRAG 2 cut(s) 1322, 1421
SsiI CCGC 7 cut(s) 352, 638, 729, 746, 777, 788, 1351
SspMI CTAG 2 cut(s) 683, 1271
StyD4I CCNGG 3 cut(s) 1089, 1156, 1393
StyI CCWWGG 3 cut(s) 315, 363, 763
TaaI ACNGT 3 cut(s) 556, 895, 1375
TaqI TCGA 2 cut(s) 105, 1251
Tru1I TTAA 5 cut(s) 137, 288, 345, 816, 912
Tru9I TTAA 5 cut(s) 137, 288, 345, 816, 912
TscAI CASTG 4 cut(s) 559, 682, 1080, 1183
TseFI GTSAC 1 cut(s) 957
TseI GCWGC 8 cut(s) 196, 207, 232, 611, 716, 719, 790, 1389
Tsp45I GTSAC 1 cut(s) 957
TspDTI ATGAA 7 cut(s) 206, 435, 602, 930, 1053, 1221, 1461
TspRI CASTG 4 cut(s) 559, 682, 1080, 1183
Van91I CCANNNNNTGG 2 cut(s) 473, 1178
VneI GTGCAC 1 cut(s) 1369
XapI RAATTY 5 cut(s) 112, 624, 732, 1030, 1354
XceI RCATGY 2 cut(s) 185, 1216
XmnI GAANNNNTTC 1 cut(s) 378
XspI CTAG 2 cut(s) 683, 1271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.