Rh6CG170900

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000440
Physical Location & Seq
Reverse (-)
23540997 .. 23545043
4047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG170900.1

Sequence Viewer

Length: 2178 bp
ATGGTCTTACTGGGTCTTGCTAGATTTGCGCTCCAATGCTTTGGTGTTCTTGCATGGCCTCTCTTTGCTCTGGTCTATCCTCTGTATTCTTCCATTAGAGCAATCGAGACAAATTCCATTACAGATACTCACAAGATAAATACGTATTGGGTCGTCTTCTCATTGATCTTGCTATTTGAAAATGCTTTTATGAAGCTCCTTGAATGGTTCCTGCTCTGGCCATACATTAGGCTACTGATTGTCTTCTTTTTGGTGATGCCACATTTTGATGGCGCCTTATATGTCTATAAACACCTTATCTGTCCATGCCTCTCCTTGGATCCCCAAATTGTTATCAACTTGTTCAACAAGCGGAAGGAGTCCCTTGCGAGAGATAACTTTCTCTCTGAGGTTGAGAGATATGTCAAGGAGAATGGACCTGAAGGTTTGGAAAAATTTATTGCTTGCAAGTCAACAAGGACAAATCTCAATGTAAAAGAGTTCAATGCAGTTTCATCCATGGATAATGAAGCGTCAAAATCCATAAAGCCAAATGGTGGACAGAATGATATCAAAGCTGTGCAGGTAATGGTGAACAAGAAGGAAACAACTGCTAGCCAAGTTAGTCAGGCAGAGCCTAATCTCACTGTGAATGAAGATAAATTTTCTTTTAGGAAGATGAATGAAAAAGCCATTGGGGCTGCAGCCGACAGAGAAATTCTGAATACTCCACCTCCAAAGAAAGTCCAGAAGGAGTGGACCACAGTCAGTCCGCTACCAACTCAAACTGATGCAACTTTTAATTCCCCTCTCGGTGATAATAAACACAAGGCTACATACGAGGCCTTGAAGATAAAGAATGAGGCAGAGCCGAAGCTCACTTGGCCTGAAAGCAGTACTGTTGCAGCCATGGAAATATATGAAAAGCCGGTTGAGGTTGGAACAGTTATAGAAGATCCCGAGCCTCCTAAAGAAGTCCAGAAAGAGTGGACTTGTGCTCTCTGTCAGGTAACTACTGCATGTGAAAGAAATATGAATTCCCACCTGCAAGGGAGAAAACACAAGGCAGCATATGAGGCACTGAAAGCAGGGAATCGGACATTTGTGCCCAAGATTGCCCCAGCTTCAACTGCAAAAACATCCAATCAACCAAATAAAGAGCCTGGAAAGAGTATACGAAGTACAGGGAAGCTCAATTGGCCTGAAATCCATACTGTTTCATTCATGGAACTATTTGAAAAGCCTGTTGAGTTTGGAACAGTTATAGAAGATCCCGAGCCTCCTAAAGAAGTACAGAAAGAGTGGACTTGTGCTCTCTGTCACGTAACTACTGCATGTGAAAGAAATATGAATTCCCACCTGCAAGGAAGAAAACACAAGGCAGCATATGAGGCACTGAAAGCAGGGAATCAGGCATTTGTGCCCAAGATTGCCCCAGCTTCAACTGCAAAAACATCCAATCAACCAAATATAGAGCCTGGAAAGAGTATACGAAGTACAGGGAAGCTCAATTGGCCTGAAATCCATACTGTTTCATTCATGGAACTATTTGAAAAGCCTGTTGAGTTTGGAACAGTTATAGAAGATCCCGAGCCTCCTAAAGTAGTACAGAAAGAGTGGACTTGTGCTCTCTGTCAGGTAGCTACTGCATGTGAAAGAAATATGAATTCCCACCTGCAAGGGAGAAAACACAAGGCAGCATATGAGGCACTGAAAGCAGGGAATCAGGCATTTGTGCCCAAGATTGCCCCAGCTTCAACTGCAAAAACATCCAATCAACCAAATAAAGAGCCTGGAAAGAGTATTCCGAGCAGTGGATCAGAACAAAAAGTTACTGTAAATGAAAATGTGCAAGGCCAGAAAAATAGTAGTCCACCTCCAATCACAAAGAAGCACTATAAACCGAAAGAGCAGCTAGCAGATGGTGTATCCAGCAATGGCCAGAAAGGGAAAATTGCTATACACGTCGAGAAGGTACAAAGTCAACAACCAAAGCCAAATGAAGTTCCAAGGGTGAAGAGTTCTCCTATGCAGTGCAAAATTTGTGGCGTATGCTGTTCTGATGAGATTGACTTGCTGTCTCATCTTAACGGAAAAAAACACCTGGAAAATGTGCAAGAGTACCTAATCAGGGAATGGATGAAAAAGCCCAGGGGCCTTTGTGATACCGAAACTGGTGAGTTTGACATTTACTGGTGA

Protein Analysis

725

Amino Acids

81.23

Weight (kDa)

8.75

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 27 - 103 1.6e-18 TB2/DP1, HVA22 family
zf-met PF12874 323 - 347 8.2e-08 Zinc-finger of C2H2 type
zf-met PF12874 428 - 452 5.4e-07 Zinc-finger of C2H2 type
zf-met PF12874 533 - 557 1.1e-06 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000440)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20313 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_5g25851
prunus_persica Prupe.8G072900_v2.0.a1 Prupe.8G072900_v2.0.a1 Prupe.8G072900_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1
pyrus_communis pycom01g01210 pycom02g12000 pycom10g21410 pycom11g11060 pycom13g24280
rosa_chinensis RchiOBHm_Chr3g0461871 RchiOBHm_Chr4g0427221 RchiOBHm_Chr4g0427231 RchiOBHm_Chr6g0270081 RchiOBHm_Chr6g0270121 RchiOBHm_Chr6g0270151 RchiOBHm_Chr6g0270161 RchiOBHm_Chr6g0270171 RchiOBHm_Chr6g0270181 RchiOBHm_Chr6g0270191 RchiOBHm_Chr7g0229301
rosa_laevigata RLG00000013809 RLG00000013810 RLG00000013812 RLG00000013814 RLG00000013817 RLG00000013818
rosa_multiflora Rmu_sc0000918.1_g000004 Rmu_sc0000918.1_g000019 Rmu_sc0000918.1_g000027 Rmu_sc0002817.1_g000013 Rmu_sc0004366.1_g000009 Rmu_sc0004376.1_g000011 Rmu_sc0004376.1_g000014 Rmu_sc0004376.1_g000021 Rmu_sc0006909.1_g000002 Rmu_sc0011356.1_g000003 Rmu_sc0011369.1_g000005 Rmu_sc0017552.1_g000001
rosa_roxburghii Rroxscaffold_5G00354200 Rroxscaffold_7G00198080 Rroxscaffold_7G00198090 Rroxscaffold_7G00198100 Rroxscaffold_7G00198130 Rroxscaffold_7G00198170
rosa_rugosa Rorug06G0052400 Rorug06G0052500 Rorug06G0053000
rosa_samantha Rh6AG171300 Rh6AG171400 Rh6AG171800 Rh6AG171900 Rh6AG172000 Rh6AG172100 Rh6AG369700 Rh6BG175800 Rh6BG175900 Rh6BG176200 Rh6BG176300 Rh6CG170900 Rh6CG171100 Rh6CG171400 Rh6CG171500 Rh6CG171700 Rh6CG171900 Rh6CG172000 Rh6CG172100
rosa_wichuraiana Rw6G014730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1032, 1347, 1662
Acc36I ACCTGC 4 cut(s) 553, 1032, 1347, 1662
AccB1I GGYRCC 1 cut(s) 272
AccB7I CCANNNNNTGG 1 cut(s) 536
AccI GTMKAC 2 cut(s) 1153, 1468
AciI CCGC 2 cut(s) 352, 752
AclWI GGATC 6 cut(s) 314, 327, 929, 1244, 1559, 1804
AcoI YGGCCR 2 cut(s) 218, 1918
AcsI RAATTY 8 cut(s) 112, 434, 641, 696, 1015, 1330, 1645, 2019
AcuI CTGAAG 1 cut(s) 441
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 7 cut(s) 877, 1162, 1272, 1477, 1587, 1956, 2102
AfiI CCNNNNNNNGG 4 cut(s) 316, 536, 1793, 2110
AflIII ACRYGT 1 cut(s) 1942
AhdI GACNNNNNGTC 1 cut(s) 2056
AjiI CACGTC 1 cut(s) 1945
AjnI CCWGG 5 cut(s) 1141, 1456, 1771, 2082, 2129
AjuI GAANNNNNNNTTGG 2 cut(s) 657, 689
Alw21I GWGCWC 3 cut(s) 979, 1294, 1609
Alw26I GTCTC 2 cut(s) 101, 2064
AlwI GGATC 6 cut(s) 314, 327, 929, 1244, 1559, 1804
Ama87I CYCGRG 3 cut(s) 938, 1253, 1568
AoxI GGCC 9 cut(s) 56, 218, 822, 863, 1178, 1493, 1834, 1918, 2134
ApeKI GCWGC 7 cut(s) 680, 683, 884, 1046, 1361, 1676, 1891
ApoI RAATTY 8 cut(s) 112, 434, 641, 696, 1015, 1330, 1645, 2019
AspLEI GCGC 2 cut(s) 31, 275
AspS9I GGNCC 3 cut(s) 416, 738, 2134
AsuHPI GGTGA 5 cut(s) 265, 583, 806, 2005, 2168
AsuNHI GCTAGC 2 cut(s) 593, 1894
AvaI CYCGRG 3 cut(s) 938, 1253, 1568
AvaII GGWCC 2 cut(s) 416, 738
BaeGI GKGCMC 3 cut(s) 1089, 1404, 1719
BalI TGGCCA 2 cut(s) 220, 1920
BamHI GGATCC 1 cut(s) 319
BanI GGYRCC 1 cut(s) 272
BbsI GAAGAC 2 cut(s) 148, 235
Bbv12I GWGCWC 3 cut(s) 979, 1294, 1609
BbvI GCAGC 7 cut(s) 667, 695, 896, 1058, 1373, 1688, 1903
BccI CCATC 2 cut(s) 263, 1895
BciT130I CCWGG 5 cut(s) 1143, 1458, 1773, 2084, 2131
BciVI GTATCC 1 cut(s) 1918
BcoDI GTCTC 2 cut(s) 101, 2064
BfaI CTAG 3 cut(s) 21, 594, 1895
BfmI CTRYAG 1 cut(s) 681
BfoI RGCGCY 1 cut(s) 276
BfuAI ACCTGC 4 cut(s) 553, 1032, 1347, 1662
BfuI GTATCC 1 cut(s) 1918
BglI GCCNNNNNGGC 1 cut(s) 677
BisI GCNGC 7 cut(s) 681, 684, 885, 1047, 1362, 1677, 1892
BlsI GCNGC 7 cut(s) 682, 685, 886, 1048, 1363, 1678, 1893
BmcAI AGTACT 1 cut(s) 877
Bme1390I CCNGG 5 cut(s) 1143, 1458, 1773, 2084, 2131
Bme18I GGWCC 2 cut(s) 416, 738
BmeRI GACNNNNNGTC 1 cut(s) 2056
BmeT110I CYCGRG 3 cut(s) 938, 1253, 1568
BmgBI CACGTC 1 cut(s) 1945
BmgT120I GGNCC 3 cut(s) 416, 738, 2134
BmiI GGNNCC 4 cut(s) 209, 274, 321, 2135
BmrFI CCNGG 5 cut(s) 1143, 1458, 1773, 2084, 2131
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 2 cut(s) 246, 760
BmtI GCTAGC 2 cut(s) 597, 1898
BmuI ACTGGG 1 cut(s) 20
BoxI GACNNNNGTC 1 cut(s) 743
BpiI GAAGAC 2 cut(s) 148, 235
BsaAI YACGTR 2 cut(s) 144, 1303
BsaHI GRCGYC 1 cut(s) 273
BsaJI CCNNGG 6 cut(s) 315, 498, 888, 1988, 2129, 2130
BsaXI ACNNNNNCTCC 2 cut(s) 697, 727
Bsc4I CCNNNNNNNGG 4 cut(s) 316, 536, 1793, 2110
Bse118I RCCGGY 1 cut(s) 907
Bse1I ACTGG 3 cut(s) 15, 2158, 2177
Bse3DI GCAATG 1 cut(s) 1921
BseBI CCWGG 5 cut(s) 1143, 1458, 1773, 2084, 2131
BseDI CCNNGG 6 cut(s) 315, 498, 888, 1988, 2129, 2130
BseGI GGATG 5 cut(s) 494, 1118, 1433, 1748, 2124
BseLI CCNNNNNNNGG 4 cut(s) 316, 536, 1793, 2110
BseMI GCAATG 1 cut(s) 1921
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 3 cut(s) 15, 2158, 2177
BseSI GKGCMC 3 cut(s) 1089, 1404, 1719
BseXI GCAGC 7 cut(s) 667, 695, 896, 1058, 1373, 1688, 1903
BseYI CCCAGC 3 cut(s) 1099, 1414, 1729
BsgI GTGCAG 1 cut(s) 581
BshFI GGCC 9 cut(s) 58, 220, 824, 865, 1180, 1495, 1836, 1920, 2136
BshNI GGYRCC 1 cut(s) 272
BsiHKAI GWGCWC 3 cut(s) 979, 1294, 1609
BsiHKCI CYCGRG 3 cut(s) 938, 1253, 1568
BsiSI CCGG 1 cut(s) 908
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 4 cut(s) 316, 536, 1793, 2110
BsmAI GTCTC 2 cut(s) 101, 2064
BsmFI GGGAC 1 cut(s) 346
BsnI GGCC 9 cut(s) 58, 220, 824, 865, 1180, 1495, 1836, 1920, 2136
BsoBI CYCGRG 3 cut(s) 938, 1253, 1568
Bsp1286I GDGCHC 6 cut(s) 979, 1089, 1294, 1404, 1609, 1719
Bsp143I GATC 6 cut(s) 165, 319, 934, 1249, 1564, 1796
Bsp19I CCATGG 2 cut(s) 498, 888
BspACI CCGC 2 cut(s) 352, 752
BspANI GGCC 9 cut(s) 58, 220, 824, 865, 1180, 1495, 1836, 1920, 2136
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 4 cut(s) 209, 274, 321, 2135
BspMAI CTGCAG 1 cut(s) 685
BspMI ACCTGC 4 cut(s) 553, 1032, 1347, 1662
BspOI GCTAGC 2 cut(s) 597, 1898
BspPI GGATC 6 cut(s) 314, 327, 929, 1244, 1559, 1804
BspT107I GGYRCC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 1921
BsrFI RCCGGY 1 cut(s) 907
BsrI ACTGG 3 cut(s) 15, 2158, 2177
BssAI RCCGGY 1 cut(s) 907
BssECI CCNNGG 6 cut(s) 315, 498, 888, 1988, 2129, 2130
BssMI GATC 6 cut(s) 165, 319, 934, 1249, 1564, 1796
BssNAI GTATAC 2 cut(s) 1154, 1469
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 4 cut(s) 315, 498, 888, 1988
Bst1107I GTATAC 2 cut(s) 1154, 1469
Bst2UI CCWGG 5 cut(s) 1143, 1458, 1773, 2084, 2131
Bst4CI ACNGT 9 cut(s) 628, 745, 880, 925, 1195, 1240, 1510, 1555, 1816
Bst6I CTCTTC 1 cut(s) 1991
BstACI GRCGYC 1 cut(s) 273
BstBAI YACGTR 2 cut(s) 144, 1303
BstC8I GCNNGC 3 cut(s) 445, 595, 1896
BstDEI CTNAG 1 cut(s) 387
BstDSI CCRYGG 2 cut(s) 498, 888
BstF5I GGATG 5 cut(s) 494, 1118, 1433, 1748, 2124
BstH2I RGCGCY 1 cut(s) 276
BstHHI GCGC 2 cut(s) 31, 275
BstKTI GATC 6 cut(s) 168, 322, 937, 1252, 1567, 1799
BstMAI GTCTC 2 cut(s) 101, 2064
BstMBI GATC 6 cut(s) 165, 319, 934, 1249, 1564, 1796
BstNI CCWGG 5 cut(s) 1143, 1458, 1773, 2084, 2131
BstNSI RCATGY 3 cut(s) 1002, 1317, 1632
BstPAI GACNNNNGTC 1 cut(s) 743
BstSCI CCNGG 5 cut(s) 1141, 1456, 1771, 2082, 2129
BstSFI CTRYAG 1 cut(s) 681
BstSLI GKGCMC 3 cut(s) 1089, 1404, 1719
BstSNI TACGTA 1 cut(s) 144
BstV1I GCAGC 7 cut(s) 667, 695, 896, 1058, 1373, 1688, 1903
BstV2I GAAGAC 2 cut(s) 148, 235
BstX2I RGATCY 4 cut(s) 319, 934, 1249, 1564
BstXI CCANNNNNNTGG 1 cut(s) 41
BstYI RGATCY 4 cut(s) 319, 934, 1249, 1564
BstZ17I GTATAC 2 cut(s) 1154, 1469
BsuI GTATCC 1 cut(s) 1918
BsuRI GGCC 9 cut(s) 58, 220, 824, 865, 1180, 1495, 1836, 1920, 2136
BtgI CCRYGG 2 cut(s) 498, 888
BtrI CACGTC 1 cut(s) 1945
BtsCI GGATG 5 cut(s) 494, 1118, 1433, 1748, 2124
BtsI GCAGTG 2 cut(s) 1798, 2018
BtsIMutI CAGTG 6 cut(s) 624, 1058, 1373, 1688, 1798, 2018
BveI ACCTGC 4 cut(s) 553, 1032, 1347, 1662
Cac8I GCNNGC 3 cut(s) 445, 595, 1896
CfoI GCGC 2 cut(s) 31, 275
Cfr10I RCCGGY 1 cut(s) 907
Cfr13I GGNCC 3 cut(s) 416, 738, 2134
CseI GACGC 1 cut(s) 501
Csp6I GTAC 7 cut(s) 876, 1161, 1271, 1476, 1586, 1955, 2101
CspCI CAANNNNNGTGG 2 cut(s) 2005, 2040
CviAII CATG 9 cut(s) 54, 306, 499, 889, 999, 1204, 1314, 1519, 1629
CviQI GTAC 7 cut(s) 876, 1161, 1271, 1476, 1586, 1955, 2101
DdeI CTNAG 1 cut(s) 387
DinI GGCGCC 1 cut(s) 274
DpnI GATC 6 cut(s) 167, 321, 936, 1251, 1566, 1798
DpnII GATC 6 cut(s) 165, 319, 934, 1249, 1564, 1796
DriI GACNNNNNGTC 1 cut(s) 2056
EaeI YGGCCR 2 cut(s) 218, 1918
Eam1104I CTCTTC 1 cut(s) 1991
Eam1105I GACNNNNNGTC 1 cut(s) 2056
EarI CTCTTC 1 cut(s) 1991
Eco105I TACGTA 1 cut(s) 144
Eco130I CCWWGG 4 cut(s) 315, 498, 888, 1988
Eco147I AGGCCT 1 cut(s) 824
Eco32I GATATC 1 cut(s) 550
Eco47I GGWCC 2 cut(s) 416, 738
Eco57I CTGAAG 1 cut(s) 441
Eco88I CYCGRG 3 cut(s) 938, 1253, 1568
EcoO109I RGGNCCY 1 cut(s) 2134
EcoRI GAATTC 3 cut(s) 1015, 1330, 1645
EcoRII CCWGG 5 cut(s) 1141, 1456, 1771, 2082, 2129
EcoRV GATATC 1 cut(s) 550
EcoT14I CCWWGG 4 cut(s) 315, 498, 888, 1988
EgeI GGCGCC 1 cut(s) 274
EheI GGCGCC 1 cut(s) 274
ErhI CCWWGG 4 cut(s) 315, 498, 888, 1988
FaeI CATG 9 cut(s) 57, 309, 502, 892, 1002, 1207, 1317, 1522, 1632
FaqI GGGAC 1 cut(s) 346
FatI CATG 9 cut(s) 53, 305, 498, 888, 998, 1203, 1313, 1518, 1628
FauNDI CATATG 3 cut(s) 1051, 1366, 1681
FblI GTMKAC 2 cut(s) 1153, 1468
Fnu4HI GCNGC 7 cut(s) 681, 684, 885, 1047, 1362, 1677, 1892
FokI GGATG 5 cut(s) 481, 1105, 1420, 1735, 2131
Fsp4HI GCNGC 7 cut(s) 681, 684, 885, 1047, 1362, 1677, 1892
FspBI CTAG 3 cut(s) 21, 594, 1895
GlaI GCGC 2 cut(s) 30, 274
GluI GCNGC 7 cut(s) 681, 684, 885, 1047, 1362, 1677, 1892
GsaI CCCAGC 3 cut(s) 1103, 1418, 1733
HaeII RGCGCY 1 cut(s) 276
HaeIII GGCC 9 cut(s) 58, 220, 824, 865, 1180, 1495, 1836, 1920, 2136
HapII CCGG 1 cut(s) 908
HgaI GACGC 1 cut(s) 501
HhaI GCGC 2 cut(s) 31, 275
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 9 cut(s) 57, 309, 502, 892, 1002, 1207, 1317, 1522, 1632
Hin6I GCGC 2 cut(s) 29, 273
HinP1I GCGC 2 cut(s) 29, 273
HincII GTYRAC 2 cut(s) 453, 1964
HindII GTYRAC 2 cut(s) 453, 1964
HinfI GANTC 4 cut(s) 359, 1072, 1387, 1702
HpaII CCGG 1 cut(s) 908
HphI GGTGA 5 cut(s) 265, 583, 806, 2005, 2168
Hpy188I TCNGA 6 cut(s) 388, 702, 1077, 1788, 1801, 2041
Hpy188III TCNNGA 7 cut(s) 106, 727, 938, 958, 1253, 1568, 1948
Hpy99I CGWCG 1 cut(s) 1949
HpyAV CCTTC 5 cut(s) 349, 416, 574, 724, 1945
HpyCH4III ACNGT 9 cut(s) 628, 745, 880, 925, 1195, 1240, 1510, 1555, 1816
HpyCH4IV ACGT 3 cut(s) 143, 1302, 1944
HpyF3I CTNAG 1 cut(s) 387
HpySE526I ACGT 3 cut(s) 143, 1302, 1944
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 9 cut(s) 57, 309, 502, 892, 1002, 1207, 1317, 1522, 1632
HspAI GCGC 2 cut(s) 29, 273
KasI GGCGCC 1 cut(s) 272
Kzo9I GATC 6 cut(s) 165, 319, 934, 1249, 1564, 1796
LmnI GCTCC 2 cut(s) 36, 201
Lsp1109I GCAGC 7 cut(s) 667, 695, 896, 1058, 1373, 1688, 1903
LweI GCATC 2 cut(s) 246, 760
MaeI CTAG 3 cut(s) 21, 594, 1895
MaeII ACGT 3 cut(s) 143, 1302, 1944
MaeIII GTNAC 4 cut(s) 988, 1298, 1303, 1810
MalI GATC 6 cut(s) 167, 321, 936, 1251, 1566, 1798
MboI GATC 6 cut(s) 165, 319, 934, 1249, 1564, 1796
MfeI CAATTG 2 cut(s) 1174, 1489
MflI RGATCY 4 cut(s) 319, 934, 1249, 1564
MhlI GDGCHC 6 cut(s) 979, 1089, 1294, 1404, 1609, 1719
MlsI TGGCCA 2 cut(s) 220, 1920
MluNI TGGCCA 2 cut(s) 220, 1920
Mly113I GGCGCC 1 cut(s) 273
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 1 cut(s) 898
Mox20I TGGCCA 2 cut(s) 220, 1920
MscI TGGCCA 2 cut(s) 220, 1920
MseI TTAA 2 cut(s) 780, 2067
MslI CAYNNNNRTG 1 cut(s) 267
Msp20I TGGCCA 2 cut(s) 220, 1920
MspI CCGG 1 cut(s) 908
MspR9I CCNGG 5 cut(s) 1143, 1458, 1773, 2084, 2131
MunI CAATTG 2 cut(s) 1174, 1489
MvaI CCWGG 5 cut(s) 1143, 1458, 1773, 2084, 2131
NarI GGCGCC 1 cut(s) 273
NcoI CCATGG 2 cut(s) 498, 888
NdeI CATATG 3 cut(s) 1051, 1366, 1681
NdeII GATC 6 cut(s) 165, 319, 934, 1249, 1564, 1796
NheI GCTAGC 2 cut(s) 593, 1894
NlaIII CATG 9 cut(s) 57, 309, 502, 892, 1002, 1207, 1317, 1522, 1632
NlaIV GGNNCC 4 cut(s) 209, 274, 321, 2135
NmuCI GTSAC 1 cut(s) 1298
NspI RCATGY 3 cut(s) 1002, 1317, 1632
PaqCI CACCTGC 3 cut(s) 1032, 1347, 1662
PasI CCCWGGG 1 cut(s) 2130
PceI AGGCCT 1 cut(s) 824
PfeI GAWTC 3 cut(s) 1072, 1387, 1702
PflMI CCANNNNNTGG 1 cut(s) 536
PkrI GCNGC 7 cut(s) 682, 685, 886, 1048, 1363, 1678, 1893
PleI GAGTC 1 cut(s) 367
PluTI GGCGCC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 367
Ppu21I YACGTR 2 cut(s) 144, 1303
PshAI GACNNNNGTC 1 cut(s) 743
Psp6I CCWGG 5 cut(s) 1141, 1456, 1771, 2082, 2129
PspFI CCCAGC 3 cut(s) 1099, 1414, 1729
PspGI CCWGG 5 cut(s) 1141, 1456, 1771, 2082, 2129
PspN4I GGNNCC 4 cut(s) 209, 274, 321, 2135
PspPI GGNCC 3 cut(s) 416, 738, 2134
PstI CTGCAG 1 cut(s) 685
PsuI RGATCY 4 cut(s) 319, 934, 1249, 1564
RsaI GTAC 7 cut(s) 877, 1162, 1272, 1477, 1587, 1956, 2102
RsaNI GTAC 7 cut(s) 876, 1161, 1271, 1476, 1586, 1955, 2101
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 2 cut(s) 780, 2067
SatI GCNGC 7 cut(s) 681, 684, 885, 1047, 1362, 1677, 1892
Sau3AI GATC 6 cut(s) 165, 319, 934, 1249, 1564, 1796
Sau96I GGNCC 3 cut(s) 416, 738, 2134
ScaI AGTACT 1 cut(s) 877
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 5 cut(s) 1143, 1458, 1773, 2084, 2131
SduI GDGCHC 6 cut(s) 979, 1089, 1294, 1404, 1609, 1719
SfaNI GCATC 2 cut(s) 246, 760
SfcI CTRYAG 1 cut(s) 681
SfoI GGCGCC 1 cut(s) 274
SinI GGWCC 2 cut(s) 416, 738
SmiMI CAYNNNNRTG 1 cut(s) 267
SnaBI TACGTA 1 cut(s) 144
SseBI AGGCCT 1 cut(s) 824
SsiI CCGC 2 cut(s) 352, 752
SspDI GGCGCC 1 cut(s) 272
SspMI CTAG 3 cut(s) 21, 594, 1895
StuI AGGCCT 1 cut(s) 824
StyD4I CCNGG 5 cut(s) 1141, 1456, 1771, 2082, 2129
StyI CCWWGG 4 cut(s) 315, 498, 888, 1988
TaaI ACNGT 9 cut(s) 628, 745, 880, 925, 1195, 1240, 1510, 1555, 1816
TaiI ACGT 3 cut(s) 146, 1305, 1947
TaqI TCGA 2 cut(s) 105, 1947
TatI WGTACW 5 cut(s) 875, 1160, 1270, 1475, 1585
TfiI GAWTC 3 cut(s) 1072, 1387, 1702
Tru1I TTAA 2 cut(s) 780, 2067
Tru9I TTAA 2 cut(s) 780, 2067
TscAI CASTG 6 cut(s) 631, 1065, 1380, 1695, 1798, 2018
TseFI GTSAC 1 cut(s) 1298
TseI GCWGC 7 cut(s) 680, 683, 884, 1046, 1361, 1676, 1891
Tsp45I GTSAC 1 cut(s) 1298
TspGWI ACGGA 1 cut(s) 2085
TspRI CASTG 6 cut(s) 631, 1065, 1380, 1695, 1798, 2018
Van91I CCANNNNNTGG 1 cut(s) 536
VpaK11BI GGWCC 2 cut(s) 416, 738
XapI RAATTY 8 cut(s) 112, 434, 641, 696, 1015, 1330, 1645, 2019
XceI RCATGY 3 cut(s) 1002, 1317, 1632
XmiI GTMKAC 2 cut(s) 1153, 1468
XspI CTAG 3 cut(s) 21, 594, 1895
ZrmI AGTACT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.