Rroxscaffold_7G00198090

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000440
Physical Location & Seq
Forward (+)
42946349 .. 42950170
3822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00198090.1

Sequence Viewer

Length: 1542 bp
ATGGGCTTACTGGGTCTTGTTGGATTTGCGCTCCAATGCTTTGTTGTTCTTGCATGGCCTCTCTTTGCTCTGGTCTATCCTCTGTATTCTTCCATTAGAGCAATCGAGACAAATTCCATTACTGATACTCACAAGATAAATACGTATTGGGTCGTCTTCTCATTGCTCTTGCTATTTGAACATGCTTTTATGAAGCTCCTTGAATGGTTCCTGCTCTGGCCATACATTAGGCTACTGATTGTCTTCTTTTTGGTGATGCCACATTTTGATGGTGCCTTATATGTCTATCAACACCTTATCTGTCCATGCCGCTCCATGGATCCCCAAATTGTTATTAACTTGTTCAACAAGCGGAAGGAGTCCCTAGTGAGAGATAACTTTCTCTTTGAGGTGGAGAGATATGTCAAGGAGAACGGACCTGAGGGTTTGGAAAAATTTATTGCTTGCAAGTCAACGAGGACAAATCTCAATGTAAAAGAGTTCAATGCAGTTTCATCCATGGATAATGAAGCGTCAAAATTGTCAAAATCCATTAAGCCAAATGGCGGACAGAATGATATCAAAGCTGTGCAGGAAATGGTGAAAAAGAAGGAAACAACTGCTAGCCAAGTTAGTCAGGCAGAGCCTGATCTCACTGTGAATGAAGATAAATTTTCTTCTAGGAAGATGGATGAAAAAATCATTGAGGTTGCAGCCAACAGAGAAATTCTTAATACTCCGCCTCCAAAGAAAGTCCAGAAGGAGTGGACCAAAGTCAGTCCGCTACCAACTCAAACTGATGCAACTTTTAATTCCCCTCTCAGTGATAATAAACACAAGGCTAAAAACGAGGCACTGAAGATGAAGAATGGGGCAGAGCCGAAGTTCACTGGGCCTGAAAACCATACTGTTGCAGCCATGGAAATATATGAAAAGCCGGTTGAGGTTGGAATAGCTATAGAAGATCCAGAGCCTCCTAAAGAAGTCCAGAAAGAGTGGACTTGTGCTCTCTGTCAGGTAACTACTTCATGTGAAAGCAATTTGAATTCCCACCTTCGAGGGAGAAAACACAAGGCAGCATATGAGGCACTGAAAGCAGGGAACCAGGCATTTATGCCCAAGATTGCCCCAGCTTCAACTGCAAAAACATCCAATCAACCAAATAAGGAGCCAGGAAAGAGTATTCCGAAGAAAATGAAAATGAAATTGTGCAAGGCCAGAAAAATAGTAGTCCAGCTCCAATCACAGAAAAGCAATCCAGCAATGGCCAGAAAGGGATCAAAACAAAAAGTTACTGTAAATGAAAATGTGCAAGGCCAGAAAAATAGTAGTCCAGCTCCAATCACAAAGAAGCAATCCAGCAATGGCCAGAAAGGGAAAATTCCTATACACGTCGAGAAGGTACAAAGTCAACAACCGAAGCCAAATGAAGTTCCAAGGGTGAAGGGTTCTCCTTTGCGGTGCGCAATCTGTAGCATACGCTGTTATGATGAGATTGCCATGCTGTCTCATCTCAAAGGAAAAAAACACAAGGAAAAGTGCAAGAACAACAAGAGAACCTAA

Protein Analysis

513

Amino Acids

57.82

Weight (kDa)

9.56

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 27 - 103 7.4e-19 TB2/DP1, HVA22 family
zf-met PF12874 326 - 350 1e-08 Zinc-finger of C2H2 type
zf-met PF12874 480 - 503 9.4e-07 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000440)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20313 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_5g25851
prunus_persica Prupe.8G072900_v2.0.a1 Prupe.8G072900_v2.0.a1 Prupe.8G072900_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1
pyrus_communis pycom01g01210 pycom02g12000 pycom10g21410 pycom11g11060 pycom13g24280
rosa_chinensis RchiOBHm_Chr3g0461871 RchiOBHm_Chr4g0427221 RchiOBHm_Chr4g0427231 RchiOBHm_Chr6g0270081 RchiOBHm_Chr6g0270121 RchiOBHm_Chr6g0270151 RchiOBHm_Chr6g0270161 RchiOBHm_Chr6g0270171 RchiOBHm_Chr6g0270181 RchiOBHm_Chr6g0270191 RchiOBHm_Chr7g0229301
rosa_laevigata RLG00000013809 RLG00000013810 RLG00000013812 RLG00000013814 RLG00000013817 RLG00000013818
rosa_multiflora Rmu_sc0000918.1_g000004 Rmu_sc0000918.1_g000019 Rmu_sc0000918.1_g000027 Rmu_sc0002817.1_g000013 Rmu_sc0004366.1_g000009 Rmu_sc0004376.1_g000011 Rmu_sc0004376.1_g000014 Rmu_sc0004376.1_g000021 Rmu_sc0006909.1_g000002 Rmu_sc0011356.1_g000003 Rmu_sc0011369.1_g000005 Rmu_sc0017552.1_g000001
rosa_roxburghii Rroxscaffold_5G00354200 Rroxscaffold_7G00198080 Rroxscaffold_7G00198090 Rroxscaffold_7G00198100 Rroxscaffold_7G00198130 Rroxscaffold_7G00198170
rosa_rugosa Rorug06G0052400 Rorug06G0052500 Rorug06G0053000
rosa_samantha Rh6AG171300 Rh6AG171400 Rh6AG171800 Rh6AG171900 Rh6AG172000 Rh6AG172100 Rh6AG369700 Rh6BG175800 Rh6BG175900 Rh6BG176200 Rh6BG176300 Rh6CG170900 Rh6CG171100 Rh6CG171400 Rh6CG171500 Rh6CG171700 Rh6CG171900 Rh6CG172000 Rh6CG172100
rosa_wichuraiana Rw6G014730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1444
AccB1I GGYRCC 1 cut(s) 272
AccBSI CCGCTC 1 cut(s) 312
AciI CCGC 6 cut(s) 310, 352, 546, 719, 761, 1438
AclWI GGATC 4 cut(s) 314, 327, 938, 1264
AcoI YGGCCR 3 cut(s) 218, 1245, 1345
AcsI RAATTY 6 cut(s) 112, 434, 650, 705, 1024, 1359
AcuI CTGAAG 1 cut(s) 857
AfaI GTAC 1 cut(s) 1383
AfiI CCNNNNNNNGG 2 cut(s) 316, 545
AflIII ACRYGT 1 cut(s) 1369
AgsI TTSAA 6 cut(s) 179, 203, 346, 484, 1024, 1116
AjiI CACGTC 1 cut(s) 1372
AjnI CCWGG 2 cut(s) 1083, 1150
AluBI AGCT 6 cut(s) 196, 566, 935, 1112, 1216, 1316
AluI AGCT 6 cut(s) 196, 566, 935, 1112, 1216, 1316
Alw21I GWGCWC 1 cut(s) 988
Alw26I GTCTC 2 cut(s) 101, 1491
AlwI GGATC 4 cut(s) 314, 327, 938, 1264
AlwNI CAGNNNCTG 1 cut(s) 626
AoxI GGCC 7 cut(s) 56, 218, 872, 1194, 1245, 1294, 1345
ApeKI GCWGC 3 cut(s) 692, 893, 1055
ApoI RAATTY 6 cut(s) 112, 434, 650, 705, 1024, 1359
AspLEI GCGC 2 cut(s) 31, 1445
AspS9I GGNCC 3 cut(s) 416, 747, 872
AsuHPI GGTGA 3 cut(s) 265, 592, 1432
AsuNHI GCTAGC 1 cut(s) 602
AvaII GGWCC 2 cut(s) 416, 747
AxyI CCTNAGG 1 cut(s) 420
BalI TGGCCA 3 cut(s) 220, 1247, 1347
BamHI GGATCC 1 cut(s) 319
BanI GGYRCC 1 cut(s) 272
BbsI GAAGAC 2 cut(s) 148, 235
Bbv12I GWGCWC 1 cut(s) 988
BbvI GCAGC 3 cut(s) 704, 905, 1067
BccI CCATC 2 cut(s) 263, 661
BciT130I CCWGG 2 cut(s) 1085, 1152
BcoDI GTCTC 2 cut(s) 101, 1491
BfaI CTAG 3 cut(s) 365, 603, 660
BfmI CTRYAG 2 cut(s) 936, 1450
BisI GCNGC 4 cut(s) 310, 693, 894, 1056
BlsI GCNGC 4 cut(s) 311, 694, 895, 1057
Bme1390I CCNGG 2 cut(s) 1085, 1152
Bme18I GGWCC 2 cut(s) 416, 747
BmgBI CACGTC 1 cut(s) 1372
BmgT120I GGNCC 3 cut(s) 416, 747, 872
BmiI GGNNCC 5 cut(s) 209, 274, 321, 1082, 1149
BmrFI CCNGG 2 cut(s) 1085, 1152
BmrI ACTGGG 2 cut(s) 20, 879
BmsI GCATC 2 cut(s) 246, 769
BmtI GCTAGC 1 cut(s) 606
BmuI ACTGGG 2 cut(s) 20, 879
BoxI GACNNNNGTC 1 cut(s) 752
BpiI GAAGAC 2 cut(s) 148, 235
BsaAI YACGTR 1 cut(s) 144
BsaJI CCNNGG 4 cut(s) 315, 498, 897, 1415
BsaXI ACNNNNNCTCC 2 cut(s) 706, 736
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 545
Bse118I RCCGGY 1 cut(s) 916
Bse1I ACTGG 2 cut(s) 15, 874
Bse21I CCTNAGG 1 cut(s) 420
Bse3DI GCAATG 3 cut(s) 161, 1248, 1348
BseBI CCWGG 2 cut(s) 1085, 1152
BseDI CCNNGG 4 cut(s) 315, 498, 897, 1415
BseGI GGATG 3 cut(s) 494, 676, 1127
BseLI CCNNNNNNNGG 2 cut(s) 316, 545
BseMI GCAATG 3 cut(s) 161, 1248, 1348
BseMII CTCAG 2 cut(s) 411, 814
BseNI ACTGG 2 cut(s) 15, 874
BseXI GCAGC 3 cut(s) 704, 905, 1067
BseYI CCCAGC 1 cut(s) 1108
BsgI GTGCAG 1 cut(s) 590
BshFI GGCC 7 cut(s) 58, 220, 874, 1196, 1247, 1296, 1347
BshNI GGYRCC 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 988
BsiSI CCGG 1 cut(s) 917
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 2 cut(s) 316, 545
BsmAI GTCTC 2 cut(s) 101, 1491
BsmFI GGGAC 1 cut(s) 346
BsnI GGCC 7 cut(s) 58, 220, 874, 1196, 1247, 1296, 1347
Bsp1286I GDGCHC 1 cut(s) 988
Bsp143I GATC 4 cut(s) 319, 628, 943, 1256
Bsp19I CCATGG 3 cut(s) 315, 498, 897
BspACI CCGC 6 cut(s) 310, 352, 546, 719, 761, 1438
BspANI GGCC 7 cut(s) 58, 220, 874, 1196, 1247, 1296, 1347
BspCNI CTCAG 2 cut(s) 412, 813
BspLI GGNNCC 5 cut(s) 209, 274, 321, 1082, 1149
BspOI GCTAGC 1 cut(s) 606
BspPI GGATC 4 cut(s) 314, 327, 938, 1264
BspT107I GGYRCC 1 cut(s) 272
BsrBI CCGCTC 1 cut(s) 312
BsrDI GCAATG 3 cut(s) 161, 1248, 1348
BsrFI RCCGGY 1 cut(s) 916
BsrI ACTGG 2 cut(s) 15, 874
BssAI RCCGGY 1 cut(s) 916
BssECI CCNNGG 4 cut(s) 315, 498, 897, 1415
BssMI GATC 4 cut(s) 319, 628, 943, 1256
BssT1I CCWWGG 4 cut(s) 315, 498, 897, 1415
Bst2UI CCWGG 2 cut(s) 1085, 1152
Bst4CI ACNGT 3 cut(s) 637, 889, 1276
BstBAI YACGTR 1 cut(s) 144
BstC8I GCNNGC 2 cut(s) 445, 604
BstDEI CTNAG 2 cut(s) 420, 800
BstDSI CCRYGG 3 cut(s) 315, 498, 897
BstF5I GGATG 3 cut(s) 494, 676, 1127
BstHHI GCGC 2 cut(s) 31, 1445
BstKTI GATC 4 cut(s) 322, 631, 946, 1259
BstMAI GTCTC 2 cut(s) 101, 1491
BstMBI GATC 4 cut(s) 319, 628, 943, 1256
BstMWI GCNNNNNNNGC 3 cut(s) 1064, 1073, 1118
BstNI CCWGG 2 cut(s) 1085, 1152
BstNSI RCATGY 1 cut(s) 185
BstPAI GACNNNNGTC 1 cut(s) 752
BstSCI CCNGG 2 cut(s) 1083, 1150
BstSFI CTRYAG 2 cut(s) 936, 1450
BstSNI TACGTA 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 704, 905, 1067
BstV2I GAAGAC 2 cut(s) 148, 235
BstX2I RGATCY 2 cut(s) 319, 943
BstYI RGATCY 2 cut(s) 319, 943
Bsu36I CCTNAGG 1 cut(s) 420
BsuRI GGCC 7 cut(s) 58, 220, 874, 1196, 1247, 1296, 1347
BtgI CCRYGG 3 cut(s) 315, 498, 897
BtrI CACGTC 1 cut(s) 1372
BtsCI GGATG 3 cut(s) 494, 676, 1127
BtsIMutI CAGTG 5 cut(s) 633, 808, 833, 867, 1067
Cac8I GCNNGC 2 cut(s) 445, 604
CaiI CAGNNNCTG 1 cut(s) 626
CfoI GCGC 2 cut(s) 31, 1445
Cfr10I RCCGGY 1 cut(s) 916
Cfr13I GGNCC 3 cut(s) 416, 747, 872
CseI GACGC 1 cut(s) 501
Csp6I GTAC 1 cut(s) 1382
CviAII CATG 8 cut(s) 54, 182, 306, 316, 499, 898, 1008, 1480
CviQI GTAC 1 cut(s) 1382
DdeI CTNAG 2 cut(s) 420, 800
DpnI GATC 4 cut(s) 321, 630, 945, 1258
DpnII GATC 4 cut(s) 319, 628, 943, 1256
EaeI YGGCCR 3 cut(s) 218, 1245, 1345
EciI GGCGGA 2 cut(s) 561, 708
Eco105I TACGTA 1 cut(s) 144
Eco130I CCWWGG 4 cut(s) 315, 498, 897, 1415
Eco32I GATATC 1 cut(s) 559
Eco47I GGWCC 2 cut(s) 416, 747
Eco57I CTGAAG 1 cut(s) 857
Eco81I CCTNAGG 1 cut(s) 420
EcoRI GAATTC 1 cut(s) 1024
EcoRII CCWGG 2 cut(s) 1083, 1150
EcoRV GATATC 1 cut(s) 559
EcoT14I CCWWGG 4 cut(s) 315, 498, 897, 1415
ErhI CCWWGG 4 cut(s) 315, 498, 897, 1415
FaeI CATG 8 cut(s) 57, 185, 309, 319, 502, 901, 1011, 1483
FaqI GGGAC 1 cut(s) 346
FatI CATG 8 cut(s) 53, 181, 305, 315, 498, 897, 1007, 1479
FauNDI CATATG 1 cut(s) 1060
Fnu4HI GCNGC 4 cut(s) 310, 693, 894, 1056
FokI GGATG 3 cut(s) 481, 683, 1114
Fsp4HI GCNGC 4 cut(s) 310, 693, 894, 1056
FspBI CTAG 3 cut(s) 365, 603, 660
FspI TGCGCA 1 cut(s) 1444
GlaI GCGC 2 cut(s) 30, 1444
GluI GCNGC 4 cut(s) 310, 693, 894, 1056
GsaI CCCAGC 1 cut(s) 1112
HaeIII GGCC 7 cut(s) 58, 220, 874, 1196, 1247, 1296, 1347
HapII CCGG 1 cut(s) 917
HgaI GACGC 1 cut(s) 501
HhaI GCGC 2 cut(s) 31, 1445
Hin1II CATG 8 cut(s) 57, 185, 309, 319, 502, 901, 1011, 1483
Hin6I GCGC 2 cut(s) 29, 1443
HinP1I GCGC 2 cut(s) 29, 1443
HincII GTYRAC 2 cut(s) 453, 1391
HindII GTYRAC 2 cut(s) 453, 1391
HinfI GANTC 1 cut(s) 359
HpaII CCGG 1 cut(s) 917
HphI GGTGA 3 cut(s) 265, 592, 1432
Hpy166II GTNNAC 5 cut(s) 453, 747, 867, 978, 1391
Hpy188I TCNGA 1 cut(s) 1167
Hpy188III TCNNGA 5 cut(s) 106, 736, 947, 967, 1375
Hpy8I GTNNAC 5 cut(s) 453, 747, 867, 978, 1391
Hpy99I CGWCG 1 cut(s) 1376
HpyAV CCTTC 6 cut(s) 349, 583, 733, 1043, 1372, 1417
HpyCH4III ACNGT 3 cut(s) 637, 889, 1276
HpyCH4IV ACGT 2 cut(s) 143, 1371
HpyF10VI GCNNNNNNNGC 3 cut(s) 1064, 1073, 1118
HpyF3I CTNAG 2 cut(s) 420, 800
HpySE526I ACGT 2 cut(s) 143, 1371
Hsp92II CATG 8 cut(s) 57, 185, 309, 319, 502, 901, 1011, 1483
HspAI GCGC 2 cut(s) 29, 1443
Kzo9I GATC 4 cut(s) 319, 628, 943, 1256
LmnI GCTCC 6 cut(s) 36, 201, 317, 1147, 1221, 1321
Lsp1109I GCAGC 3 cut(s) 704, 905, 1067
LweI GCATC 2 cut(s) 246, 769
MaeI CTAG 3 cut(s) 365, 603, 660
MaeII ACGT 2 cut(s) 143, 1371
MaeIII GTNAC 2 cut(s) 997, 1270
MalI GATC 4 cut(s) 321, 630, 945, 1258
MbiI CCGCTC 1 cut(s) 312
MboI GATC 4 cut(s) 319, 628, 943, 1256
MflI RGATCY 2 cut(s) 319, 943
MhlI GDGCHC 1 cut(s) 988
MlsI TGGCCA 3 cut(s) 220, 1247, 1347
MluNI TGGCCA 3 cut(s) 220, 1247, 1347
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 1 cut(s) 907
Mox20I TGGCCA 3 cut(s) 220, 1247, 1347
MscI TGGCCA 3 cut(s) 220, 1247, 1347
MseI TTAA 4 cut(s) 336, 534, 711, 789
MslI CAYNNNNRTG 1 cut(s) 267
Msp20I TGGCCA 3 cut(s) 220, 1247, 1347
MspI CCGG 1 cut(s) 917
MspR9I CCNGG 2 cut(s) 1085, 1152
MvaI CCWGG 2 cut(s) 1085, 1152
MwoI GCNNNNNNNGC 3 cut(s) 1064, 1073, 1118
NcoI CCATGG 3 cut(s) 315, 498, 897
NdeI CATATG 1 cut(s) 1060
NdeII GATC 4 cut(s) 319, 628, 943, 1256
NheI GCTAGC 1 cut(s) 602
NlaIII CATG 8 cut(s) 57, 185, 309, 319, 502, 901, 1011, 1483
NlaIV GGNNCC 5 cut(s) 209, 274, 321, 1082, 1149
NsbI TGCGCA 1 cut(s) 1444
NspI RCATGY 1 cut(s) 185
PkrI GCNGC 4 cut(s) 311, 694, 895, 1057
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
Ppu21I YACGTR 1 cut(s) 144
PshAI GACNNNNGTC 1 cut(s) 752
Psp6I CCWGG 2 cut(s) 1083, 1150
PspFI CCCAGC 1 cut(s) 1108
PspGI CCWGG 2 cut(s) 1083, 1150
PspN4I GGNNCC 5 cut(s) 209, 274, 321, 1082, 1149
PspPI GGNCC 3 cut(s) 416, 747, 872
PstNI CAGNNNCTG 1 cut(s) 626
PsuI RGATCY 2 cut(s) 319, 943
RsaI GTAC 1 cut(s) 1383
RsaNI GTAC 1 cut(s) 1382
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 4 cut(s) 336, 534, 711, 789
SatI GCNGC 4 cut(s) 310, 693, 894, 1056
Sau3AI GATC 4 cut(s) 319, 628, 943, 1256
Sau96I GGNCC 3 cut(s) 416, 747, 872
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 1085, 1152
SduI GDGCHC 1 cut(s) 988
SfaNI GCATC 2 cut(s) 246, 769
SfcI CTRYAG 2 cut(s) 936, 1450
SinI GGWCC 2 cut(s) 416, 747
SmiMI CAYNNNNRTG 1 cut(s) 267
SnaBI TACGTA 1 cut(s) 144
SsiI CCGC 6 cut(s) 310, 352, 546, 719, 761, 1438
SspMI CTAG 3 cut(s) 365, 603, 660
StyD4I CCNGG 2 cut(s) 1083, 1150
StyI CCWWGG 4 cut(s) 315, 498, 897, 1415
TaaI ACNGT 3 cut(s) 637, 889, 1276
TaiI ACGT 2 cut(s) 146, 1374
TaqI TCGA 3 cut(s) 105, 1036, 1374
TauI GCSGC 1 cut(s) 312
Tru1I TTAA 4 cut(s) 336, 534, 711, 789
Tru9I TTAA 4 cut(s) 336, 534, 711, 789
TscAI CASTG 5 cut(s) 640, 808, 840, 874, 1074
TseI GCWGC 3 cut(s) 692, 893, 1055
TspGWI ACGGA 1 cut(s) 429
TspRI CASTG 5 cut(s) 640, 808, 840, 874, 1074
VpaK11BI GGWCC 2 cut(s) 416, 747
XapI RAATTY 6 cut(s) 112, 434, 650, 705, 1024, 1359
XceI RCATGY 1 cut(s) 185
XspI CTAG 3 cut(s) 365, 603, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.