Rh6BG176300

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000440
Physical Location & Seq
Reverse (-)
29802179 .. 29883611
81433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG176300.1

Sequence Viewer

Length: 4959 bp
ATGGGCTTACTGGGTCTCGTTGGATTAGCGCTCCAATGCTTTGATGTTCTTGCATGGCCTCTCTTTGCTCTGGTCTATCCTCTGTATTCTTCCATTAGAGCAATCGAGACAAATTCCATTACGGATACTCACAAGATAAATACGTATTGGGTCGTCTTCTCATTGATCTTGCTATTTGAACATGCTTTTATGAAGCTCCTTGAATGGTTCCTGCTCTGGCCATACATTAGGCTACTGATTGTCTTCTTTTTGGTGATGCCACATTTTGATGGTGCCTTATATGTCTATAAACACCTTATCTGTCCATGCCTCTCCATGGATCCCCAAATTGTTATCAACTTGTTCAACAAGCGGAAGGAGTCCCTTGTGAGAGATAACTTTCTCTCTGAGGTGGAGAGATATGTAAAGGAGAATGGACCTGAAGGTTTGGAAAGATTTATTGCTTGCAAGTCAACGAGGACAAATCTCGATGTAAAAGAGTTCAATGCAGTTTCATCCATGGATACTGAAGCGTCAAAATCCATAAAGCCAAATGGTGGACAAAATGATATCAAAGCTGTGAAGGTAATGGCAAAGAAGAAGCGAACAACTGCTAGTCAAGTTAGTCAGGCAGAGCCTAATCTCACTGTGAATGAAGATAAATTTTCTTCTATGAAGATGAATGACAAAGCCATTGAGGGTGCAGCACATAGAAAAATTCTGAAGACACCACCTCCAAAGAAAGTCCAGAAGGAGTGGACCACCACTCAAACTGATGCAACTTTGAGTTCCCCTCTCAGTGATAATAAAGACAAGGCTACATATGAGGCACGGAAGATAAAGAATGAGGCAGATCCTAAGCTCACGTGGCCTGAAAACCGTGCTGCTGCAGCTATGGAAATATATGAAAAGCCAGTTGAGGTTGGAAAAGGTATAGAAGATCCAGAGACTCCTAAAGAAGTCCAGAAAGAGTGGACTTGTGCTCTCTGTCAAGTAACCACTACATGCGAAAGCAATATGAATTGCCACCTGCAAGGGCGAAAACACAAGGCAACATGTGAGGCAGTGAAAGCAAGGAACCAGGATTTTTTGCCCAAGATTGCCCCAGCTTCAACTGCAAACACATCCAATCAACCGAATGAGGTGCCAGGAAAGAGTTCCCCGGGCAGTGGTTCAAAACAAAAGGTTACTATCAGTGAAAATTCGAAATCCATAGAGGCAAATGTTGGACAGAATGATATCAAAGCTGTGCAGGTAACGGCAAAAAAGAAGGAAACAACTTCTAGCCAAGTTTGTCAGGCCGAGCCTAATCTCACTGTGAATGAAGATAAATTTTCTTCTACGAAGATGAATGACAAAGCCATTGACGGTGCTGCACATAGAGAAATTCTGAAGACACCACCTCCAAAGAAAGTCCAGAAGGAGTGGACCACCACTCAAACTGATGCAACTTTGAGTTCCCCTCTCGGTGATAATAAAGACATGGCTACATACGAGGCACTGAAGATAAAGAATGAGGCAGATCCTAAGCTCACTTGGCCCGAAAACCGTGCTGCTGCAGCTATGGAAATATATGAAAAGCCAGTTGTGGTTGGAAAAGGTATAGAAGATCCAGAGACTCCTAAAGAAGTCCAGAAAGAGTGGACTTGTGCTCTCTGTCAAGTAACCACTACATGTGAAAGCAATTTGCATTCCCACCTGCAAGGGCGAAAACACAAGGCAGCATATGAGGCAGTGAAAGCAAGGAACCAGGAATTTTTGCCCAAGATTGCCCCAGCGTCATCTGCAAAAACATCCAATCAACCGAATGAGGTGCCAGGAAAGAGTTCCCCTAGCAGTGGTTCAAAACAAAAGGTTACTATCAGTCAAAATTCGAAATCCATAGAGGCAAATGTTGGACAGAATGATATCAAAGCTGTGCAGGTAACGGCAAAGAAGAAGGAAACAACTTCTAGCCAAGTTTGTCAGGCCGAGCCTAATCTCACTGTGAATGAAGATAAATTTTCTTGTATGAAGATGAATGAAAAAGCCATAGAGGGTGCAGCCGATAGAGAAATTCTGAAGACACCACCTCCAAAGAAAGTCCAGAAGGAGTGGACCACAGTTAGTCCGCTACAAACTCAAACTGATGCAACTTTGTGTTCCCCTCCTGGTGATAATAAAGGCAAGGCTACATACGTGGCGCTGAAGATAGAGAATGAGGCAGAGGCTAAGCTCACTTGGACTGAAAACCGTGCTGTTGCAGCCATGGAAATATATGAAATTCAGGTTGAGGTTGGAACAGGTATTGAAGATCCAGAGACTCCTAAAGAAGTCCAGAAAGAGTGGACTTGTACTCTGTGTCAAGTAACCACTACATGTGGAAGCAATTTGAATTCCCACCTGCAAGGGCGAAAACACAAGGCAGCATATGAGGCAGTGAAAGCAAGGAACCAGGAATTTTTGCCCAAGATTGCCCCAGCTTCAACTGCAAAAACATCCAATCAACCGAATGAGGTGCCAGGAAAGAGTTCCCCGAGCAGTGGATCAAAACAAAAGTTGAAATCCATTGAGGCAAATGGTGGACAGAATGATATCAAAGCTTTGCAGGTAACGGCAAAGAAGAAGGAAACAACTTCTAGCCAAGTTTGTCAGGCCGAGCCTAATCTCACTGTGAATGAAGATAAATTTTCTTGTACGAAGATGAATGAAAAAGCCATAGAGGGTGCAGCCGATAGAGAAATTCTGAAGACACCACCTCCAAAGAAAGTCCAGAAGGAGTGGACCACCGTTAGTCCGCTACAAACTCAAACTGATGCAACTTTGTGTTCCCCTCCTGGTGATAATAAAGGCAAGGCTACATACGTGGCGCTGAAGATAGAGAATGAGGCAGAGGCTAAGCTCACTTGGACTGAAAACTGTGCTGTTGCAGCCATGGAAATATATGAAATTCAGGTTGAGGTTGGAACAGGTATTGAAGATCCAGAGACTTCTAAAGAAGTCCAGAAAGAGTGGACTTGTACTCTGTGTCAAGTAACCACTACATGTGAAAGCAATTTGAATTCCCACCTGCAAGGGCGAAAACACAAGGCAGCATATGAGGCAGTGAAAGCAAGGAACCAGGAATTTTTGCCCAAGATTGCCCCAGCTTCAACTGCAAAAACATCCAATCAACCGAATGAGGTGCCAGGAAAGAGTTCCCCGAGCAGTGGATCAAAACAAAAGTTGAAATCCATTGAGGCAAATGGTGGACAGAATGATATCAAAGCTGTGCAGGTAACGGCAAAAAAGAAGGAAACAACTTCTAGCCAAGTTTGTCAGGCCGAGCCTAATCTCACTGTGAATGAAGATAAATTTTCTTTTACGAAGATGAATGAAAAAGCCATTGAGGGTGCAGCCGATAGAGAAATTCTGAAGACACCACCTCCAAAGAAAGTCCAGAAGGAGTGGACCACAGTCAGTCTGCTACAAACTCAAACTGATGCAACTTTGAGTTCCCCTCTCAGTGATAATAAAGACAAGGCTACATACGAGGCACGGAAGATAAAGAATGAGGCAGATCCTAAGCTCACGTGGCCTGAAAACCGTGCTGCTGCAGCTATGGAAATATATGAAAAGCCAGTTGAGGTTGGTAAAGGTATAGAAGATCCAGAGACTCCTAAAGAAGTCCAGACAGAGTGGACTTGTGCTCTCTGTCAAGTAACCACTACATGTGAAAGCAATTTGAATTCCCACTTGCAAGGGCGAAAACACAAGGCAGCATATGAGGCAGTGAAAGGAAGGAACCAGGAGTTTTTGCCCAAGATTGCCCCAGCTTCAACTGCAAAAACATCCAATCAACCAAATGAGGTGCCAGGAAAGAGTTCCCCGAGCAGTGGATCAAAACCAAAAGTTCCTATGAATGAAAATTCGAAATCCATAGAGGCAAATGGTGGACGGAATGATATTAAAGCTTTGCTGGTAACGGCAAAAAGGAAGGAAACAACTGGTAGCCAAATAAGGTTTTACATGTGGGAAAATCTTATAACTGCATTGTTGGTTATAAATATGTCCACGGTTAGTCAGGCAGAGCCTAATCTCACTGTGAATGAAGATAAATTTTCTTCTAGGAAGATGAATGAAAAAGTCATCGAAGTTGCAGCCAACAGAGAAATTCTGAAGACTCCACCTCCAAAGAAAGTCCAGAAGGAGTGGACCTCAGTCAATCCGCTGCAAACTCAAACTGATGCAACGTTGACTTCCCTTCTCGGTGCTAATAAACATGAGGCTACATACGAGGCACTGAAGATAAAGAATGAGGCAGAGCCTAAGCTCACTGGGCCTGAAAACCGTACTGTTGCAGCCATGGAAATAAATGAAAAGCCGGTTGAGGTTGGAACAGGTATAGAAGATCCAGAGTCTCCTAAAGAAGTCCAGAAAGAGTGGACTTGTGCTCTCTGTCAGGTAACTACTACATGTGAAAGAAATTTGAATTCCCACCTGCGAGGGAGAAAACACAAGGCAGCATATGAGGCACTGAAAGGGAACCAGGCATTTGTGCCCAAGATTGCCCCAGCTTCAACCGCAAAAACATCCAATCAACCAAATAAGGAGCCAGGAAAGAGTATTCCGAAGAAAATGGAAATGAAAATGAAAATGAAAATGTGCAAGGCCAGAAAAATAGTAGTCCAGCTCCAATCACAAAGAAGCAATCCAACAATGGCCAGAAAGGGATCAAAACAAAAAGTTACTGTAAATGAAAATGTGCAAGGCCAGAAAAATAGTAGTCCAGCTCCAATCACAAAGAAGCAATCCAGCAATGGCCAGAAAGGGAAAATTGCTATACAGGTCGAGAAGGTACAAAGTCAACAACCGAAGCCAAATGAAGTTCCAAGGGTGAAGAGTTCTCCTTTGCGGTGCGCAATTTGTAGAATACGCTGTGCTGGTGAGATTGACATGCTGTCTCATCTCAACGGAAGAAAACACAAGGAAAATGTGCAAGAACGAAAAAAGAGAACCTAA

Protein Analysis

1652

Amino Acids

182.26

Weight (kDa)

9.09

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 27 - 103 4.6e-18 TB2/DP1, HVA22 family
zf-met PF12874 318 - 342 1.1e-07 Zinc-finger of C2H2 type
zf-met PF12874 541 - 565 1.5e-07 Zinc-finger of C2H2 type
zf-met PF12874 769 - 793 4.4e-07 Zinc-finger of C2H2 type
zf-met PF12874 991 - 1015 1.3e-08 Zinc-finger of C2H2 type
zf-met PF12874 1213 - 1237 6.4e-09 Zinc-finger of C2H2 type
zf-met PF12874 1461 - 1485 8.2e-08 Zinc-finger of C2H2 type
zf-met PF12874 1618 - 1641 2.5e-06 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000440)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20313 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_5g25851
prunus_persica Prupe.8G072900_v2.0.a1 Prupe.8G072900_v2.0.a1 Prupe.8G072900_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1
pyrus_communis pycom01g01210 pycom02g12000 pycom10g21410 pycom11g11060 pycom13g24280
rosa_chinensis RchiOBHm_Chr3g0461871 RchiOBHm_Chr4g0427221 RchiOBHm_Chr4g0427231 RchiOBHm_Chr6g0270081 RchiOBHm_Chr6g0270121 RchiOBHm_Chr6g0270151 RchiOBHm_Chr6g0270161 RchiOBHm_Chr6g0270171 RchiOBHm_Chr6g0270181 RchiOBHm_Chr6g0270191 RchiOBHm_Chr7g0229301
rosa_laevigata RLG00000013809 RLG00000013810 RLG00000013812 RLG00000013814 RLG00000013817 RLG00000013818
rosa_multiflora Rmu_sc0000918.1_g000004 Rmu_sc0000918.1_g000019 Rmu_sc0000918.1_g000027 Rmu_sc0002817.1_g000013 Rmu_sc0004366.1_g000009 Rmu_sc0004376.1_g000011 Rmu_sc0004376.1_g000014 Rmu_sc0004376.1_g000021 Rmu_sc0006909.1_g000002 Rmu_sc0011356.1_g000003 Rmu_sc0011369.1_g000005 Rmu_sc0017552.1_g000001
rosa_roxburghii Rroxscaffold_5G00354200 Rroxscaffold_7G00198080 Rroxscaffold_7G00198090 Rroxscaffold_7G00198100 Rroxscaffold_7G00198130 Rroxscaffold_7G00198170
rosa_rugosa Rorug06G0052400 Rorug06G0052500 Rorug06G0053000
rosa_samantha Rh6AG171300 Rh6AG171400 Rh6AG171800 Rh6AG171900 Rh6AG172000 Rh6AG172100 Rh6AG369700 Rh6BG175800 Rh6BG175900 Rh6BG176200 Rh6BG176300 Rh6CG170900 Rh6CG171100 Rh6CG171400 Rh6CG171500 Rh6CG171700 Rh6CG171900 Rh6CG172000 Rh6CG172100
rosa_wichuraiana Rw6G014730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3983, 4001
AarI CACCTGC 5 cut(s) 1017, 1686, 2370, 3036, 4446
Acc16I TGCGCA 1 cut(s) 4858
Acc36I ACCTGC 9 cut(s) 1017, 1222, 1686, 1891, 2370, 2557, 3036, 3223, 4446
AccB1I GGYRCC 6 cut(s) 272, 1123, 1792, 2476, 3142, 3808
AccB7I CCANNNNNTGG 1 cut(s) 536
AciI CCGC 6 cut(s) 352, 2090, 2756, 4166, 4521, 4852
AclI AACGTT 1 cut(s) 4190
AcoI YGGCCR 3 cut(s) 218, 4659, 4759
AcvI CACGTG 2 cut(s) 846, 3531
AfaI GTAC 5 cut(s) 2314, 2656, 2980, 4291, 4797
AfeI AGCGCT 1 cut(s) 30
AfiI CCNNNNNNNGG 7 cut(s) 316, 536, 1148, 1817, 2501, 3167, 3833
AflIII ACRYGT 7 cut(s) 1034, 1652, 2336, 3002, 3668, 3966, 4412
AhdI GACNNNNNGTC 1 cut(s) 4897
Alw21I GWGCWC 4 cut(s) 964, 1633, 3649, 4393
Alw26I GTCTC 9 cut(s) 20, 101, 920, 1589, 2273, 2939, 3605, 4362, 4905
Ama87I CYCGRG 4 cut(s) 1141, 2494, 3160, 3826
Aor51HI AGCGCT 1 cut(s) 30
Asp700I GAANNNNTTC 5 cut(s) 1135, 1804, 2488, 3154, 3820
AspLEI GCGC 4 cut(s) 31, 2164, 2830, 4859
AspS9I GGNCC 9 cut(s) 416, 738, 1407, 1518, 2076, 2742, 3408, 4152, 4277
AsuC2I CCSGG 2 cut(s) 1142, 1143
AsuHPI GGTGA 6 cut(s) 265, 1460, 2144, 2810, 4846, 4895
AsuII TTCGAA 3 cut(s) 1184, 1853, 3869
AvaI CYCGRG 4 cut(s) 1141, 2494, 3160, 3826
AvaII GGWCC 7 cut(s) 416, 738, 1407, 2076, 2742, 3408, 4152
BaeGI GKGCMC 1 cut(s) 4500
BalI TGGCCA 3 cut(s) 220, 4661, 4761
BamHI GGATCC 1 cut(s) 319
BanI GGYRCC 6 cut(s) 272, 1123, 1792, 2476, 3142, 3808
BbrPI CACGTG 2 cut(s) 846, 3531
BbsI GAAGAC 8 cut(s) 148, 235, 710, 1379, 2048, 2714, 3380, 4124
Bbv12I GWGCWC 4 cut(s) 964, 1633, 3649, 4393
BccI CCATC 1 cut(s) 263
BceAI ACGGC 5 cut(s) 1254, 1923, 2589, 3255, 3939
BciVI GTATCC 2 cut(s) 118, 496
BcnI CCSGG 2 cut(s) 1142, 1143
BcoDI GTCTC 9 cut(s) 20, 101, 920, 1589, 2273, 2939, 3605, 4362, 4905
BfaI CTAG 7 cut(s) 594, 1263, 1812, 1932, 2598, 3264, 4065
BfmI CTRYAG 3 cut(s) 867, 1536, 3552
BfoI RGCGCY 3 cut(s) 32, 2165, 2831
BfuAI ACCTGC 9 cut(s) 1017, 1222, 1686, 1891, 2370, 2557, 3036, 3223, 4446
BfuI GTATCC 2 cut(s) 118, 496
BlpI GCTNAGC 2 cut(s) 2190, 2856
Bme18I GGWCC 7 cut(s) 416, 738, 1407, 2076, 2742, 3408, 4152
BmeRI GACNNNNNGTC 1 cut(s) 4897
BmeT110I CYCGRG 4 cut(s) 1141, 2494, 3160, 3826
BmgT120I GGNCC 9 cut(s) 416, 738, 1407, 1518, 2076, 2742, 3408, 4152, 4277
BmrI ACTGGG 2 cut(s) 20, 4284
BmsI GCATC 7 cut(s) 246, 745, 1414, 2098, 2764, 3430, 4174
BmuI ACTGGG 2 cut(s) 20, 4284
BoxI GACNNNNGTC 2 cut(s) 3413, 4157
BpiI GAAGAC 8 cut(s) 148, 235, 710, 1379, 2048, 2714, 3380, 4124
BplI GAGNNNNNCTC 8 cut(s) 757, 789, 1426, 1458, 3442, 3474, 4139, 4171
Bpu10I CCTNAGC 4 cut(s) 837, 1506, 3522, 4266
Bpu1102I GCTNAGC 2 cut(s) 2190, 2856
Bpu14I TTCGAA 3 cut(s) 1184, 1853, 3869
BpuMI CCSGG 2 cut(s) 1142, 1143
BsaAI YACGTR 5 cut(s) 144, 846, 2158, 2824, 3531
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 9 cut(s) 315, 498, 1140, 1141, 2226, 2892, 4011, 4302, 4829
Bsc4I CCNNNNNNNGG 7 cut(s) 316, 536, 1148, 1817, 2501, 3167, 3833
Bse118I RCCGGY 1 cut(s) 4321
Bse1I ACTGG 6 cut(s) 15, 893, 1562, 3578, 3949, 4279
Bse3DI GCAATG 1 cut(s) 4762
BseDI CCNNGG 9 cut(s) 315, 498, 1140, 1141, 2226, 2892, 4011, 4302, 4829
BseGI GGATG 7 cut(s) 494, 1103, 1772, 2456, 3122, 3788, 4529
BseLI CCNNNNNNNGG 7 cut(s) 316, 536, 1148, 1817, 2501, 3167, 3833
BseMI GCAATG 1 cut(s) 4762
BseMII CTCAG 4 cut(s) 378, 790, 3475, 4170
BseNI ACTGG 6 cut(s) 15, 893, 1562, 3578, 3949, 4279
BseSI GKGCMC 1 cut(s) 4500
BseYI CCCAGC 6 cut(s) 1084, 1753, 2437, 3103, 3769, 4510
BsgI GTGCAG 8 cut(s) 702, 1250, 1338, 1919, 2040, 2706, 3251, 3372
BshNI GGYRCC 6 cut(s) 272, 1123, 1792, 2476, 3142, 3808
BsiHKAI GWGCWC 4 cut(s) 964, 1633, 3649, 4393
BsiHKCI CYCGRG 4 cut(s) 1141, 2494, 3160, 3826
BsiSI CCGG 2 cut(s) 1142, 4322
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 7 cut(s) 316, 536, 1148, 1817, 2501, 3167, 3833
BsmAI GTCTC 9 cut(s) 20, 101, 920, 1589, 2273, 2939, 3605, 4362, 4905
BsmFI GGGAC 1 cut(s) 346
BsmI GAATGC 1 cut(s) 1669
Bso31I GGTCTC 1 cut(s) 20
BsoBI CYCGRG 4 cut(s) 1141, 2494, 3160, 3826
Bsp119I TTCGAA 3 cut(s) 1184, 1853, 3869
Bsp1286I GDGCHC 5 cut(s) 964, 1633, 3649, 4393, 4500
Bsp1720I GCTNAGC 2 cut(s) 2190, 2856
Bsp19I CCATGG 5 cut(s) 315, 498, 2226, 2892, 4302
BspACI CCGC 6 cut(s) 352, 2090, 2756, 4166, 4521, 4852
BspCNI CTCAG 4 cut(s) 379, 789, 3474, 4169
BspMAI CTGCAG 3 cut(s) 871, 1540, 3556
BspMI ACCTGC 9 cut(s) 1017, 1222, 1686, 1891, 2370, 2557, 3036, 3223, 4446
BspT104I TTCGAA 3 cut(s) 1184, 1853, 3869
BspT107I GGYRCC 6 cut(s) 272, 1123, 1792, 2476, 3142, 3808
BspTNI GGTCTC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 4762
BsrFI RCCGGY 1 cut(s) 4321
BsrI ACTGG 6 cut(s) 15, 893, 1562, 3578, 3949, 4279
BssAI RCCGGY 1 cut(s) 4321
BssECI CCNNGG 9 cut(s) 315, 498, 1140, 1141, 2226, 2892, 4011, 4302, 4829
BssT1I CCWWGG 6 cut(s) 315, 498, 2226, 2892, 4302, 4829
Bst6I CTCTTC 1 cut(s) 4832
BstBAI YACGTR 5 cut(s) 144, 846, 2158, 2824, 3531
BstBI TTCGAA 3 cut(s) 1184, 1853, 3869
BstC8I GCNNGC 1 cut(s) 445
BstDSI CCRYGG 6 cut(s) 315, 498, 2226, 2892, 4011, 4302
BstF5I GGATG 7 cut(s) 494, 1103, 1772, 2456, 3122, 3788, 4529
BstH2I RGCGCY 3 cut(s) 32, 2165, 2831
BstHHI GCGC 4 cut(s) 31, 2164, 2830, 4859
BstMAI GTCTC 9 cut(s) 20, 101, 920, 1589, 2273, 2939, 3605, 4362, 4905
BstPAI GACNNNNGTC 2 cut(s) 3413, 4157
BstSFI CTRYAG 3 cut(s) 867, 1536, 3552
BstSLI GKGCMC 1 cut(s) 4500
BstSNI TACGTA 1 cut(s) 144
BstV2I GAAGAC 8 cut(s) 148, 235, 710, 1379, 2048, 2714, 3380, 4124
BsuI GTATCC 2 cut(s) 118, 496
BtgI CCRYGG 6 cut(s) 315, 498, 2226, 2892, 4011, 4302
BtsCI GGATG 7 cut(s) 494, 1103, 1772, 2456, 3122, 3788, 4529
BveI ACCTGC 9 cut(s) 1017, 1222, 1686, 1891, 2370, 2557, 3036, 3223, 4446
Cac8I GCNNGC 1 cut(s) 445
CfoI GCGC 4 cut(s) 31, 2164, 2830, 4859
Cfr10I RCCGGY 1 cut(s) 4321
Cfr13I GGNCC 9 cut(s) 416, 738, 1407, 1518, 2076, 2742, 3408, 4152, 4277
Cfr9I CCCGGG 1 cut(s) 1141
CseI GACGC 2 cut(s) 501, 1746
Csp6I GTAC 5 cut(s) 2313, 2655, 2979, 4290, 4796
CviQI GTAC 5 cut(s) 2313, 2655, 2979, 4290, 4796
DriI GACNNNNNGTC 1 cut(s) 4897
EaeI YGGCCR 3 cut(s) 218, 4659, 4759
Eam1104I CTCTTC 1 cut(s) 4832
Eam1105I GACNNNNNGTC 1 cut(s) 4897
EarI CTCTTC 1 cut(s) 4832
Eco105I TACGTA 1 cut(s) 144
Eco130I CCWWGG 6 cut(s) 315, 498, 2226, 2892, 4302, 4829
Eco31I GGTCTC 1 cut(s) 20
Eco32I GATATC 5 cut(s) 550, 1219, 1888, 2554, 3220
Eco47I GGWCC 7 cut(s) 416, 738, 1407, 2076, 2742, 3408, 4152
Eco47III AGCGCT 1 cut(s) 30
Eco72I CACGTG 2 cut(s) 846, 3531
Eco88I CYCGRG 4 cut(s) 1141, 2494, 3160, 3826
EcoRI GAATTC 4 cut(s) 2353, 3019, 3685, 4429
EcoRV GATATC 5 cut(s) 550, 1219, 1888, 2554, 3220
EcoT14I CCWWGG 6 cut(s) 315, 498, 2226, 2892, 4302, 4829
ErhI CCWWGG 6 cut(s) 315, 498, 2226, 2892, 4302, 4829
FaqI GGGAC 1 cut(s) 346
FauNDI CATATG 6 cut(s) 802, 1705, 2389, 3055, 3721, 4465
FokI GGATG 7 cut(s) 481, 1090, 1759, 2443, 3109, 3775, 4516
FspBI CTAG 7 cut(s) 594, 1263, 1812, 1932, 2598, 3264, 4065
FspI TGCGCA 1 cut(s) 4858
GlaI GCGC 4 cut(s) 30, 2163, 2829, 4858
GsaI CCCAGC 6 cut(s) 1088, 1757, 2441, 3107, 3773, 4514
HaeII RGCGCY 3 cut(s) 32, 2165, 2831
HapII CCGG 2 cut(s) 1142, 4322
HgaI GACGC 2 cut(s) 501, 1746
HhaI GCGC 4 cut(s) 31, 2164, 2830, 4859
Hin6I GCGC 4 cut(s) 29, 2162, 2828, 4857
HinP1I GCGC 4 cut(s) 29, 2162, 2828, 4857
HincII GTYRAC 3 cut(s) 453, 4194, 4805
HindII GTYRAC 3 cut(s) 453, 4194, 4805
HindIII AAGCTT 2 cut(s) 2559, 3909
HinfI GANTC 7 cut(s) 359, 928, 1597, 2281, 3613, 4120, 4355
HpaII CCGG 2 cut(s) 1142, 4322
HphI GGTGA 6 cut(s) 265, 1460, 2144, 2810, 4846, 4895
Hpy188I TCNGA 8 cut(s) 388, 702, 1371, 2040, 2706, 3372, 4116, 4569
HpyCH4IV ACGT 6 cut(s) 143, 845, 2157, 2823, 3530, 4190
HpySE526I ACGT 6 cut(s) 143, 845, 2157, 2823, 3530, 4190
HspAI GCGC 4 cut(s) 29, 2162, 2828, 4857
LmnI GCTCC 5 cut(s) 36, 201, 4549, 4635, 4735
LweI GCATC 7 cut(s) 246, 745, 1414, 2098, 2764, 3430, 4174
MaeI CTAG 7 cut(s) 594, 1263, 1812, 1932, 2598, 3264, 4065
MaeII ACGT 6 cut(s) 143, 845, 2157, 2823, 3530, 4190
MhlI GDGCHC 5 cut(s) 964, 1633, 3649, 4393, 4500
MlsI TGGCCA 3 cut(s) 220, 4661, 4761
MluNI TGGCCA 3 cut(s) 220, 4661, 4761
MlyI GAGTC 7 cut(s) 368, 922, 1591, 2275, 3607, 4114, 4364
MmeI TCCRAC 8 cut(s) 883, 1186, 1552, 1855, 2236, 2902, 4312, 4676
Mox20I TGGCCA 3 cut(s) 220, 4661, 4761
MroXI GAANNNNTTC 5 cut(s) 1135, 1804, 2488, 3154, 3820
MscI TGGCCA 3 cut(s) 220, 4661, 4761
MseI TTAA 1 cut(s) 3906
MslI CAYNNNNRTG 1 cut(s) 267
Msp20I TGGCCA 3 cut(s) 220, 4661, 4761
MspA1I CMGCKG 1 cut(s) 4168
MspI CCGG 2 cut(s) 1142, 4322
Mva1269I GAATGC 1 cut(s) 1669
NciI CCSGG 2 cut(s) 1142, 1143
NcoI CCATGG 5 cut(s) 315, 498, 2226, 2892, 4302
NdeI CATATG 6 cut(s) 802, 1705, 2389, 3055, 3721, 4465
NmeAIII GCCGAG 4 cut(s) 1306, 1975, 2641, 3307
NsbI TGCGCA 1 cut(s) 4858
NspV TTCGAA 3 cut(s) 1184, 1853, 3869
PaqCI CACCTGC 5 cut(s) 1017, 1686, 2370, 3036, 4446
PciI ACATGT 7 cut(s) 1034, 1652, 2336, 3002, 3668, 3966, 4412
PctI GAATGC 1 cut(s) 1669
PdmI GAANNNNTTC 5 cut(s) 1135, 1804, 2488, 3154, 3820
PflMI CCANNNNNTGG 1 cut(s) 536
PleI GAGTC 7 cut(s) 367, 922, 1591, 2275, 3607, 4114, 4363
PmaCI CACGTG 2 cut(s) 846, 3531
PmlI CACGTG 2 cut(s) 846, 3531
PpsI GAGTC 7 cut(s) 367, 922, 1591, 2275, 3607, 4114, 4363
Ppu21I YACGTR 5 cut(s) 144, 846, 2158, 2824, 3531
PscI ACATGT 7 cut(s) 1034, 1652, 2336, 3002, 3668, 3966, 4412
PshAI GACNNNNGTC 2 cut(s) 3413, 4157
PsiI TTATAA 2 cut(s) 3983, 4001
Psp1406I AACGTT 1 cut(s) 4190
PspCI CACGTG 2 cut(s) 846, 3531
PspFI CCCAGC 6 cut(s) 1084, 1753, 2437, 3103, 3769, 4510
PspPI GGNCC 9 cut(s) 416, 738, 1407, 1518, 2076, 2742, 3408, 4152, 4277
PstI CTGCAG 3 cut(s) 871, 1540, 3556
RsaI GTAC 5 cut(s) 2314, 2656, 2980, 4291, 4797
RsaNI GTAC 5 cut(s) 2313, 2655, 2979, 4290, 4796
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 1 cut(s) 3906
Sau96I GGNCC 9 cut(s) 416, 738, 1407, 1518, 2076, 2742, 3408, 4152, 4277
SchI GAGTC 7 cut(s) 368, 922, 1591, 2275, 3607, 4114, 4364
SduI GDGCHC 5 cut(s) 964, 1633, 3649, 4393, 4500
SfaNI GCATC 7 cut(s) 246, 745, 1414, 2098, 2764, 3430, 4174
SfcI CTRYAG 3 cut(s) 867, 1536, 3552
SfuI TTCGAA 3 cut(s) 1184, 1853, 3869
SinI GGWCC 7 cut(s) 416, 738, 1407, 2076, 2742, 3408, 4152
SmaI CCCGGG 1 cut(s) 1143
SmiMI CAYNNNNRTG 1 cut(s) 267
SnaBI TACGTA 1 cut(s) 144
SsiI CCGC 6 cut(s) 352, 2090, 2756, 4166, 4521, 4852
SspMI CTAG 7 cut(s) 594, 1263, 1812, 1932, 2598, 3264, 4065
StyI CCWWGG 6 cut(s) 315, 498, 2226, 2892, 4302, 4829
TaiI ACGT 6 cut(s) 146, 848, 2160, 2826, 3533, 4193
TaqI TCGA 7 cut(s) 105, 468, 1184, 1853, 3869, 4089, 4788
TatI WGTACW 2 cut(s) 2312, 2978
Tru1I TTAA 1 cut(s) 3906
Tru9I TTAA 1 cut(s) 3906
TspGWI ACGGA 5 cut(s) 137, 826, 3511, 3910, 4926
TspMI CCCGGG 1 cut(s) 1141
Van91I CCANNNNNTGG 1 cut(s) 536
VpaK11BI GGWCC 7 cut(s) 416, 738, 1407, 2076, 2742, 3408, 4152
XmaI CCCGGG 1 cut(s) 1141
XmnI GAANNNNTTC 5 cut(s) 1135, 1804, 2488, 3154, 3820
XspI CTAG 7 cut(s) 594, 1263, 1812, 1932, 2598, 3264, 4065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.