RLG00000013817

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000440
Physical Location & Seq
Forward (+)
41762574 .. 41765453
2880 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013817

Sequence Viewer

Length: 1491 bp
ATGGGCTTACTCAGCCTTGTTGGATTTGCGCTCCAATGCTTTGATGTTCTTGCATGGCCTCTCTTTGCTCTGGTCTATCCTCTGTATTCTTCCATTAAAGCAATCGAGACAAATTCCATTACAGAAACTCACAAGATAAATAAGTATTGGGTCGTCTTCTCATTGATCTTGCTATTTGAGCATGCTTTTATGAAGCTCCTTGAAAGGTTCCTGCTCTGGCCATACATTAGGCTACTGATTGTCTTCTTTTTGGTGATGCCACATTTTGATGGTGCCTTATATGTCTATAAACACCTTATCTGTCCATGCCTCTCCATGGATCCCCAAATTGTTATCAACTTGTTCAACAAGCGGAAGGAGTCCCTTGCGAGAGATAACTTTCTCTCTGAGGTTGAGAGATATGTCAAGGAGAATGGACCTGAAGGTTTGGAAAAATTTATTGCTTGCAAGTCAACGAGGACAAATCTCAATGTAAAAGAGTTCAATGCAGTTTCATCCATGGATAATGAAGCGTCAAAATCCATAAAGCCAAATGGTGGACAGAAAGATCTCAAAGCTGTGCAGGTAATGGTGAAAAAGAAGGAAACAACTGCTAGCCAAGCTAGTCAGGCAGAGCCTAATCTCACTGTGAATGAAGATAAATTTTCTTCTCGGAAGATGAATGAAAAATTCATTGAGGCTGCAGCCGACAGAGAAATTCTGAATACTCCACCTCCAAAGAAAGTCCAGAAGGAGTGGACCACAGTCAGTCCACTACCAACTCAAACTGATGCAACTTTTAATTCCCCTCTCGGTGATAATAAACACAAGGCTACATACGAGGCCTTGAAGATAAAGAATGAGGCAGAGCCGAAGCTCACTTGGCCTGAAAACAGTACTGTTGCAGCCATGGAAATATATGAAAAGCCGGTTGAGGTTGGAACAGTTATAGAAGATCCAGAGCCTCCTAAAGCAGTCCAGAAAGAGTGGACTTGTGCTCTGTCTCAGGTAACTACTACTACATGTGAAAGAAATTTGAATTCCCACCTGCAAGGGAGAAAACACAAGGCAGCATATGAGGCACTGAAAGCAGGGAACCAGGCATTTGTGCCCAAGATTGCCCCAGCTTCAACTGCAATAACATCCAATCAACCAAATAAGGAGCCAGGAAAGAGTATTCTAAATGAAAATGTGCAAGGCCAGAAAAATAGTAGTCCAGCTCCAACCACAAAGAAGCACTATAAACCGAAAGAGGAGCTAGCAGATGGTGTATCCAGCAATGGCCAGAAAAGGAAAATTGCTATACAGGTCGAGAAGGTACAAAGTCAAGAACGGAAGCCAAATGAAGTTCCAAGGGTGAAGAGTTGTCCTTTGCGGTGTGAGATTTGTAGCGTAAGCTGTTCTGATGAGATTGACTTGCTGTCTCATCTCAACGGAAAAAAACACAAGGAAAATGTGCAAGAACAACAAGAGTACCTAATCAGGGTTGGAGAGTTTGACATTTACTGGTGA

Protein Analysis

497

Amino Acids

56.04

Weight (kDa)

8.67

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 27 - 103 3.1e-17 TB2/DP1, HVA22 family
zf-C2H2_jaz PF12171 451 - 476 6.9e-06 Zinc-finger double-stranded RNA-binding
zf-met PF12874 452 - 475 7.7e-08 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000440)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20313 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_5g25851
prunus_persica Prupe.8G072900_v2.0.a1 Prupe.8G072900_v2.0.a1 Prupe.8G072900_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1
pyrus_communis pycom01g01210 pycom02g12000 pycom10g21410 pycom11g11060 pycom13g24280
rosa_chinensis RchiOBHm_Chr3g0461871 RchiOBHm_Chr4g0427221 RchiOBHm_Chr4g0427231 RchiOBHm_Chr6g0270081 RchiOBHm_Chr6g0270121 RchiOBHm_Chr6g0270151 RchiOBHm_Chr6g0270161 RchiOBHm_Chr6g0270171 RchiOBHm_Chr6g0270181 RchiOBHm_Chr6g0270191 RchiOBHm_Chr7g0229301
rosa_laevigata RLG00000013809 RLG00000013810 RLG00000013812 RLG00000013814 RLG00000013817 RLG00000013818
rosa_multiflora Rmu_sc0000918.1_g000004 Rmu_sc0000918.1_g000019 Rmu_sc0000918.1_g000027 Rmu_sc0002817.1_g000013 Rmu_sc0004366.1_g000009 Rmu_sc0004376.1_g000011 Rmu_sc0004376.1_g000014 Rmu_sc0004376.1_g000021 Rmu_sc0006909.1_g000002 Rmu_sc0011356.1_g000003 Rmu_sc0011369.1_g000005 Rmu_sc0017552.1_g000001
rosa_roxburghii Rroxscaffold_5G00354200 Rroxscaffold_7G00198080 Rroxscaffold_7G00198090 Rroxscaffold_7G00198100 Rroxscaffold_7G00198130 Rroxscaffold_7G00198170
rosa_rugosa Rorug06G0052400 Rorug06G0052500 Rorug06G0053000
rosa_samantha Rh6AG171300 Rh6AG171400 Rh6AG171800 Rh6AG171900 Rh6AG172000 Rh6AG172100 Rh6AG369700 Rh6BG175800 Rh6BG175900 Rh6BG176200 Rh6BG176300 Rh6CG170900 Rh6CG171100 Rh6CG171400 Rh6CG171500 Rh6CG171700 Rh6CG171900 Rh6CG172000 Rh6CG172100
rosa_wichuraiana Rw6G014730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1035
Acc36I ACCTGC 2 cut(s) 553, 1035
AccB1I GGYRCC 1 cut(s) 272
AccB7I CCANNNNNTGG 1 cut(s) 536
AciI CCGC 2 cut(s) 352, 1354
AclWI GGATC 3 cut(s) 314, 327, 929
AcoI YGGCCR 2 cut(s) 218, 1261
AcsI RAATTY 7 cut(s) 112, 434, 641, 668, 696, 1012, 1018
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 3 cut(s) 877, 1299, 1454
AfiI CCNNNNNNNGG 3 cut(s) 316, 536, 1462
AflIII ACRYGT 1 cut(s) 1001
AgsI TTSAA 6 cut(s) 203, 346, 484, 829, 1018, 1110
AhdI GACNNNNNGTC 1 cut(s) 1399
AjnI CCWGG 2 cut(s) 1077, 1144
AluBI AGCT 8 cut(s) 196, 557, 602, 856, 1106, 1199, 1237, 1377
AluI AGCT 8 cut(s) 196, 557, 602, 856, 1106, 1199, 1237, 1377
Alw21I GWGCWC 1 cut(s) 979
Alw26I GTCTC 3 cut(s) 101, 987, 1407
AlwI GGATC 3 cut(s) 314, 327, 929
AoxI GGCC 6 cut(s) 56, 218, 822, 863, 1177, 1261
ApeKI GCWGC 4 cut(s) 680, 683, 884, 1049
ApoI RAATTY 7 cut(s) 112, 434, 641, 668, 696, 1012, 1018
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 2 cut(s) 416, 738
AsuHPI GGTGA 4 cut(s) 265, 583, 806, 1348
AsuNHI GCTAGC 2 cut(s) 593, 1237
AvaII GGWCC 2 cut(s) 416, 738
BaeGI GKGCMC 1 cut(s) 1092
BaeI ACNNNNGTAYC 2 cut(s) 1436, 1469
BalI TGGCCA 2 cut(s) 220, 1263
BamHI GGATCC 1 cut(s) 319
BanI GGYRCC 1 cut(s) 272
BbsI GAAGAC 2 cut(s) 148, 235
Bbv12I GWGCWC 1 cut(s) 979
BbvI GCAGC 4 cut(s) 667, 695, 896, 1061
BccI CCATC 2 cut(s) 263, 1238
BciT130I CCWGG 2 cut(s) 1079, 1146
BciVI GTATCC 1 cut(s) 1261
BcoDI GTCTC 3 cut(s) 101, 987, 1407
BfaI CTAG 3 cut(s) 594, 603, 1238
BfmI CTRYAG 1 cut(s) 681
BfuAI ACCTGC 2 cut(s) 553, 1035
BfuI GTATCC 1 cut(s) 1261
BglII AGATCT 1 cut(s) 547
BisI GCNGC 4 cut(s) 681, 684, 885, 1050
BlsI GCNGC 4 cut(s) 682, 685, 886, 1051
BmcAI AGTACT 1 cut(s) 877
Bme1390I CCNGG 2 cut(s) 1079, 1146
Bme18I GGWCC 2 cut(s) 416, 738
BmeRI GACNNNNNGTC 1 cut(s) 1399
BmgT120I GGNCC 2 cut(s) 416, 738
BmiI GGNNCC 5 cut(s) 209, 274, 321, 1076, 1143
BmrFI CCNGG 2 cut(s) 1079, 1146
BmsI GCATC 2 cut(s) 246, 760
BmtI GCTAGC 2 cut(s) 597, 1241
BoxI GACNNNNGTC 1 cut(s) 743
BpiI GAAGAC 2 cut(s) 148, 235
BsaJI CCNNGG 4 cut(s) 315, 498, 888, 1331
BsaXI ACNNNNNCTCC 2 cut(s) 697, 727
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 536, 1462
Bse118I RCCGGY 1 cut(s) 907
Bse1I ACTGG 1 cut(s) 1490
Bse3DI GCAATG 1 cut(s) 1264
BseBI CCWGG 2 cut(s) 1079, 1146
BseDI CCNNGG 4 cut(s) 315, 498, 888, 1331
BseGI GGATG 2 cut(s) 494, 1121
BseLI CCNNNNNNNGG 3 cut(s) 316, 536, 1462
BseMI GCAATG 1 cut(s) 1264
BseMII CTCAG 3 cut(s) 25, 378, 998
BseNI ACTGG 1 cut(s) 1490
BseRI GAGGAG 1 cut(s) 1247
BseSI GKGCMC 1 cut(s) 1092
BseXI GCAGC 4 cut(s) 667, 695, 896, 1061
BseYI CCCAGC 1 cut(s) 1102
BsgI GTGCAG 1 cut(s) 581
BshFI GGCC 6 cut(s) 58, 220, 824, 865, 1179, 1263
BshNI GGYRCC 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 979
BsiSI CCGG 1 cut(s) 908
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 3 cut(s) 316, 536, 1462
BsmAI GTCTC 3 cut(s) 101, 987, 1407
BsmFI GGGAC 1 cut(s) 346
BsnI GGCC 6 cut(s) 58, 220, 824, 865, 1179, 1263
Bsp1286I GDGCHC 2 cut(s) 979, 1092
Bsp143I GATC 4 cut(s) 165, 319, 547, 934
Bsp19I CCATGG 3 cut(s) 315, 498, 888
BspACI CCGC 2 cut(s) 352, 1354
BspANI GGCC 6 cut(s) 58, 220, 824, 865, 1179, 1263
BspCNI CTCAG 3 cut(s) 24, 379, 997
BspLI GGNNCC 5 cut(s) 209, 274, 321, 1076, 1143
BspMAI CTGCAG 1 cut(s) 685
BspMI ACCTGC 2 cut(s) 553, 1035
BspOI GCTAGC 2 cut(s) 597, 1241
BspPI GGATC 3 cut(s) 314, 327, 929
BspT107I GGYRCC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 1264
BsrFI RCCGGY 1 cut(s) 907
BsrI ACTGG 1 cut(s) 1490
BssAI RCCGGY 1 cut(s) 907
BssECI CCNNGG 4 cut(s) 315, 498, 888, 1331
BssMI GATC 4 cut(s) 165, 319, 547, 934
BssT1I CCWWGG 4 cut(s) 315, 498, 888, 1331
Bst2UI CCWGG 2 cut(s) 1079, 1146
Bst4CI ACNGT 5 cut(s) 628, 745, 875, 880, 925
Bst6I CTCTTC 1 cut(s) 1334
BstC8I GCNNGC 4 cut(s) 183, 445, 595, 1239
BstDEI CTNAG 3 cut(s) 11, 387, 984
BstDSI CCRYGG 3 cut(s) 315, 498, 888
BstF5I GGATG 2 cut(s) 494, 1121
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 4 cut(s) 168, 322, 550, 937
BstMAI GTCTC 3 cut(s) 101, 987, 1407
BstMBI GATC 4 cut(s) 165, 319, 547, 934
BstMWI GCNNNNNNNGC 8 cut(s) 12, 178, 599, 608, 862, 1058, 1067, 1112
BstNI CCWGG 2 cut(s) 1079, 1146
BstNSI RCATGY 2 cut(s) 185, 1005
BstPAI GACNNNNGTC 1 cut(s) 743
BstSCI CCNGG 2 cut(s) 1077, 1144
BstSFI CTRYAG 1 cut(s) 681
BstSLI GKGCMC 1 cut(s) 1092
BstV1I GCAGC 4 cut(s) 667, 695, 896, 1061
BstV2I GAAGAC 2 cut(s) 148, 235
BstX2I RGATCY 3 cut(s) 319, 547, 934
BstYI RGATCY 3 cut(s) 319, 547, 934
BsuI GTATCC 1 cut(s) 1261
BsuRI GGCC 6 cut(s) 58, 220, 824, 865, 1179, 1263
BtgI CCRYGG 3 cut(s) 315, 498, 888
BtsCI GGATG 2 cut(s) 494, 1121
BtsIMutI CAGTG 2 cut(s) 624, 1061
BveI ACCTGC 2 cut(s) 553, 1035
Cac8I GCNNGC 4 cut(s) 183, 445, 595, 1239
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 907
Cfr13I GGNCC 2 cut(s) 416, 738
CseI GACGC 1 cut(s) 501
Csp6I GTAC 3 cut(s) 876, 1298, 1453
CviAII CATG 7 cut(s) 54, 182, 306, 316, 499, 889, 1002
CviQI GTAC 3 cut(s) 876, 1298, 1453
DdeI CTNAG 3 cut(s) 11, 387, 984
DpnI GATC 4 cut(s) 167, 321, 549, 936
DpnII GATC 4 cut(s) 165, 319, 547, 934
DriI GACNNNNNGTC 1 cut(s) 1399
EaeI YGGCCR 2 cut(s) 218, 1261
Eam1104I CTCTTC 1 cut(s) 1334
Eam1105I GACNNNNNGTC 1 cut(s) 1399
EarI CTCTTC 1 cut(s) 1334
Eco130I CCWWGG 4 cut(s) 315, 498, 888, 1331
Eco147I AGGCCT 1 cut(s) 824
Eco47I GGWCC 2 cut(s) 416, 738
Eco57I CTGAAG 1 cut(s) 441
EcoRI GAATTC 1 cut(s) 1018
EcoRII CCWGG 2 cut(s) 1077, 1144
EcoT14I CCWWGG 4 cut(s) 315, 498, 888, 1331
ErhI CCWWGG 4 cut(s) 315, 498, 888, 1331
FaeI CATG 7 cut(s) 57, 185, 309, 319, 502, 892, 1005
FaqI GGGAC 1 cut(s) 346
FatI CATG 7 cut(s) 53, 181, 305, 315, 498, 888, 1001
FauNDI CATATG 1 cut(s) 1054
Fnu4HI GCNGC 4 cut(s) 681, 684, 885, 1050
FokI GGATG 2 cut(s) 481, 1108
Fsp4HI GCNGC 4 cut(s) 681, 684, 885, 1050
FspBI CTAG 3 cut(s) 594, 603, 1238
GlaI GCGC 1 cut(s) 30
GluI GCNGC 4 cut(s) 681, 684, 885, 1050
GsaI CCCAGC 1 cut(s) 1106
HaeIII GGCC 6 cut(s) 58, 220, 824, 865, 1179, 1263
HapII CCGG 1 cut(s) 908
HgaI GACGC 1 cut(s) 501
HhaI GCGC 1 cut(s) 31
Hin1II CATG 7 cut(s) 57, 185, 309, 319, 502, 892, 1005
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HinfI GANTC 1 cut(s) 359
HpaII CCGG 1 cut(s) 908
HphI GGTGA 4 cut(s) 265, 583, 806, 1348
Hpy166II GTNNAC 5 cut(s) 453, 539, 738, 752, 969
Hpy188I TCNGA 4 cut(s) 388, 654, 702, 1384
Hpy188III TCNNGA 6 cut(s) 106, 727, 938, 958, 1291, 1307
Hpy8I GTNNAC 5 cut(s) 453, 539, 738, 752, 969
HpyAV CCTTC 5 cut(s) 349, 416, 574, 724, 1288
HpyCH4III ACNGT 5 cut(s) 628, 745, 875, 880, 925
HpyF10VI GCNNNNNNNGC 8 cut(s) 12, 178, 599, 608, 862, 1058, 1067, 1112
HpyF3I CTNAG 3 cut(s) 11, 387, 984
Hsp92II CATG 7 cut(s) 57, 185, 309, 319, 502, 892, 1005
HspAI GCGC 1 cut(s) 29
Kzo9I GATC 4 cut(s) 165, 319, 547, 934
LmnI GCTCC 5 cut(s) 36, 201, 1141, 1204, 1234
Lsp1109I GCAGC 4 cut(s) 667, 695, 896, 1061
LweI GCATC 2 cut(s) 246, 760
MaeI CTAG 3 cut(s) 594, 603, 1238
MaeIII GTNAC 1 cut(s) 988
MalI GATC 4 cut(s) 167, 321, 549, 936
MboI GATC 4 cut(s) 165, 319, 547, 934
MboII GAAGA 9 cut(s) 81, 148, 235, 639, 647, 667, 841, 944, 1351
MflI RGATCY 3 cut(s) 319, 547, 934
MhlI GDGCHC 2 cut(s) 979, 1092
MlsI TGGCCA 2 cut(s) 220, 1263
MluNI TGGCCA 2 cut(s) 220, 1263
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 3 cut(s) 898, 1226, 1447
Mox20I TGGCCA 2 cut(s) 220, 1263
MscI TGGCCA 2 cut(s) 220, 1263
MseI TTAA 2 cut(s) 96, 780
MslI CAYNNNNRTG 1 cut(s) 267
Msp20I TGGCCA 2 cut(s) 220, 1263
MspI CCGG 1 cut(s) 908
MspR9I CCNGG 2 cut(s) 1079, 1146
MvaI CCWGG 2 cut(s) 1079, 1146
MwoI GCNNNNNNNGC 8 cut(s) 12, 178, 599, 608, 862, 1058, 1067, 1112
NcoI CCATGG 3 cut(s) 315, 498, 888
NdeI CATATG 1 cut(s) 1054
NdeII GATC 4 cut(s) 165, 319, 547, 934
NheI GCTAGC 2 cut(s) 593, 1237
NlaIII CATG 7 cut(s) 57, 185, 309, 319, 502, 892, 1005
NlaIV GGNNCC 5 cut(s) 209, 274, 321, 1076, 1143
NspI RCATGY 2 cut(s) 185, 1005
PaeI GCATGC 1 cut(s) 185
PaqCI CACCTGC 1 cut(s) 1035
PceI AGGCCT 1 cut(s) 824
PciI ACATGT 1 cut(s) 1001
PflMI CCANNNNNTGG 1 cut(s) 536
PkrI GCNGC 4 cut(s) 682, 685, 886, 1051
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
PscI ACATGT 1 cut(s) 1001
PshAI GACNNNNGTC 1 cut(s) 743
Psp6I CCWGG 2 cut(s) 1077, 1144
PspFI CCCAGC 1 cut(s) 1102
PspGI CCWGG 2 cut(s) 1077, 1144
PspN4I GGNNCC 5 cut(s) 209, 274, 321, 1076, 1143
PspPI GGNCC 2 cut(s) 416, 738
PstI CTGCAG 1 cut(s) 685
PsuI RGATCY 3 cut(s) 319, 547, 934
RsaI GTAC 3 cut(s) 877, 1299, 1454
RsaNI GTAC 3 cut(s) 876, 1298, 1453
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 2 cut(s) 96, 780
SatI GCNGC 4 cut(s) 681, 684, 885, 1050
Sau3AI GATC 4 cut(s) 165, 319, 547, 934
Sau96I GGNCC 2 cut(s) 416, 738
ScaI AGTACT 1 cut(s) 877
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 1079, 1146
SduI GDGCHC 2 cut(s) 979, 1092
SfaNI GCATC 2 cut(s) 246, 760
SfcI CTRYAG 1 cut(s) 681
SinI GGWCC 2 cut(s) 416, 738
SmiMI CAYNNNNRTG 1 cut(s) 267
SphI GCATGC 1 cut(s) 185
SseBI AGGCCT 1 cut(s) 824
SsiI CCGC 2 cut(s) 352, 1354
SspMI CTAG 3 cut(s) 594, 603, 1238
StuI AGGCCT 1 cut(s) 824
StyD4I CCNGG 2 cut(s) 1077, 1144
StyI CCWWGG 4 cut(s) 315, 498, 888, 1331
TaaI ACNGT 5 cut(s) 628, 745, 875, 880, 925
TaqI TCGA 2 cut(s) 105, 1290
TatI WGTACW 1 cut(s) 875
Tru1I TTAA 2 cut(s) 96, 780
Tru9I TTAA 2 cut(s) 96, 780
TscAI CASTG 2 cut(s) 631, 1068
TseI GCWGC 4 cut(s) 680, 683, 884, 1049
TspGWI ACGGA 2 cut(s) 1327, 1428
TspRI CASTG 2 cut(s) 631, 1068
Van91I CCANNNNNTGG 1 cut(s) 536
VpaK11BI GGWCC 2 cut(s) 416, 738
XapI RAATTY 7 cut(s) 112, 434, 641, 668, 696, 1012, 1018
XceI RCATGY 2 cut(s) 185, 1005
XspI CTAG 3 cut(s) 594, 603, 1238
ZrmI AGTACT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.