Rh6AG171300

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000440
Physical Location & Seq
Reverse (-)
27778656 .. 27779567
912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG171300.1

Sequence Viewer

Length: 912 bp
ATGAATGAAGATAAATTTTCTTCTAGGAAGATGAATGAAAAAGCCATTGAGGCTGCAGCCGACAGAGAAATTCTAAACACTCCACCTCCAAAGAAAGTCGAGAAGCAGTGGACCACAATCAGTCCGCTACCAACTCAAACTGATGCAACTTTTAATTCCCCTCTCCGTGATAATAAACACATGGCCACATACGAGGCACTGAAGATAAAGAATGAGGAAGAACCGAAGCTCACTAGGCCAGCCATGGAAATATATGAAAAGCCGGATGAGGGAACAGCTATAGAAGATCCAGAGCCCCCTAAAGAAGTCCAGAAAGAGTGGACTTGTGCTCTCTGTCAGGTAACTACTGCATGTGAAAGAAATATGAATTCCCACCTTCAAGGGAGAAAACACAAGGCTGCATATGAGGCACTGAAGGCAGGGAACCAGGCATTTGTGCCCAAGATTGCCCCAGCTTCAACTTCAAAAACATCCAATCAACCAAATAAGGAGCCAAGAAAGAGTATTCTGAGCCGTGGATCAAAACAAAAAGTTACTGTAAATGAAAATGTGCAAGGCCAGAAAAATAGTAGTCCAGCTCCAATCACAAAGAAGCGCTATAAACCGAAAGAGCAGCTAGCAGATGGTGTGTCCAGCAATGGCCAGAAAAGGAAAATTTCTATACACGTCGAGAAGGTACAAAGTCAACACCCAAAGCCAAATGAAGTTCCAAGGGTGAAGAGTTCTCCTATGCGGTGCAATTTTTGTGGCGTATGCTGTTCTGATGAGATTGACTTGCTGTCTCATCTCAACGGAAAAAAACACAAGGAAAATGTGCAAGAGTACCTAATCAGGGTTGAAAGGATGAAAAAGCCTAGGGGCCTTTGTGATACCGAAACTGGTGAGTTTTACATGGTATCTCATCTTAATTGA

Protein Analysis

303

Amino Acids

34.24

Weight (kDa)

9.27

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-met PF12874 107 - 131 2.8e-08 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000440)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20313 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11420 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_2g11450 FvH4_5g25851
prunus_persica Prupe.8G072900_v2.0.a1 Prupe.8G072900_v2.0.a1 Prupe.8G072900_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G073000_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1 Prupe.8G229300_v2.0.a1
pyrus_communis pycom01g01210 pycom02g12000 pycom10g21410 pycom11g11060 pycom13g24280
rosa_chinensis RchiOBHm_Chr3g0461871 RchiOBHm_Chr4g0427221 RchiOBHm_Chr4g0427231 RchiOBHm_Chr6g0270081 RchiOBHm_Chr6g0270121 RchiOBHm_Chr6g0270151 RchiOBHm_Chr6g0270161 RchiOBHm_Chr6g0270171 RchiOBHm_Chr6g0270181 RchiOBHm_Chr6g0270191 RchiOBHm_Chr7g0229301
rosa_laevigata RLG00000013809 RLG00000013810 RLG00000013812 RLG00000013814 RLG00000013817 RLG00000013818
rosa_multiflora Rmu_sc0000918.1_g000004 Rmu_sc0000918.1_g000019 Rmu_sc0000918.1_g000027 Rmu_sc0002817.1_g000013 Rmu_sc0004366.1_g000009 Rmu_sc0004376.1_g000011 Rmu_sc0004376.1_g000014 Rmu_sc0004376.1_g000021 Rmu_sc0006909.1_g000002 Rmu_sc0011356.1_g000003 Rmu_sc0011369.1_g000005 Rmu_sc0017552.1_g000001
rosa_roxburghii Rroxscaffold_5G00354200 Rroxscaffold_7G00198080 Rroxscaffold_7G00198090 Rroxscaffold_7G00198100 Rroxscaffold_7G00198130 Rroxscaffold_7G00198170
rosa_rugosa Rorug06G0052400 Rorug06G0052500 Rorug06G0053000
rosa_samantha Rh6AG171300 Rh6AG171400 Rh6AG171800 Rh6AG171900 Rh6AG172000 Rh6AG172100 Rh6AG369700 Rh6BG175800 Rh6BG175900 Rh6BG176200 Rh6BG176300 Rh6CG170900 Rh6CG171100 Rh6CG171400 Rh6CG171500 Rh6CG171700 Rh6CG171900 Rh6CG172000 Rh6CG172100
rosa_wichuraiana Rw6G014730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 125, 733
AclWI GGATC 2 cut(s) 281, 526
AcoI YGGCCR 2 cut(s) 183, 640
AcsI RAATTY 4 cut(s) 14, 69, 367, 654
AcuI CTGAAG 2 cut(s) 221, 434
AfaI GTAC 2 cut(s) 678, 824
AfeI AGCGCT 1 cut(s) 596
AfiI CCNNNNNNNGG 2 cut(s) 269, 832
AflIII ACRYGT 1 cut(s) 664
AgsI TTSAA 4 cut(s) 380, 459, 465, 839
AhdI GACNNNNNGTC 1 cut(s) 778
AjiI CACGTC 1 cut(s) 667
AjnI CCWGG 1 cut(s) 426
AluBI AGCT 5 cut(s) 229, 278, 455, 578, 616
AluI AGCT 5 cut(s) 229, 278, 455, 578, 616
Alw21I GWGCWC 1 cut(s) 331
Alw26I GTCTC 1 cut(s) 786
AlwI GGATC 2 cut(s) 281, 526
Aor51HI AGCGCT 1 cut(s) 596
AoxI GGCC 5 cut(s) 183, 236, 556, 640, 859
ApeKI GCWGC 4 cut(s) 53, 56, 398, 613
ApoI RAATTY 4 cut(s) 14, 69, 367, 654
AspA2I CCTAGG 1 cut(s) 854
AspLEI GCGC 1 cut(s) 597
AspS9I GGNCC 2 cut(s) 111, 859
AsuHPI GGTGA 2 cut(s) 727, 893
AsuNHI GCTAGC 1 cut(s) 616
AvaII GGWCC 1 cut(s) 111
AvrII CCTAGG 1 cut(s) 854
BaeGI GKGCMC 1 cut(s) 441
BalI TGGCCA 2 cut(s) 185, 642
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 4 cut(s) 40, 68, 385, 625
BccI CCATC 1 cut(s) 617
BceAI ACGGC 1 cut(s) 498
BciT130I CCWGG 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 786
BfaI CTAG 4 cut(s) 24, 234, 617, 855
BfmI CTRYAG 2 cut(s) 54, 279
BfoI RGCGCY 1 cut(s) 598
BglI GCCNNNNNGGC 1 cut(s) 50
BisI GCNGC 4 cut(s) 54, 57, 399, 614
BlnI CCTAGG 1 cut(s) 854
BlsI GCNGC 4 cut(s) 55, 58, 400, 615
Bme1390I CCNGG 1 cut(s) 428
Bme18I GGWCC 1 cut(s) 111
BmeRI GACNNNNNGTC 1 cut(s) 778
BmgBI CACGTC 1 cut(s) 667
BmgT120I GGNCC 2 cut(s) 111, 859
BmiI GGNNCC 3 cut(s) 425, 492, 860
BmrFI CCNGG 1 cut(s) 428
BmsI GCATC 1 cut(s) 133
BmtI GCTAGC 1 cut(s) 620
BsaJI CCNNGG 4 cut(s) 243, 514, 710, 854
BsaXI ACNNNNNCTCC 2 cut(s) 70, 100
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 832
Bse1I ACTGG 1 cut(s) 883
Bse3DI GCAATG 1 cut(s) 643
BseBI CCWGG 1 cut(s) 428
BseDI CCNNGG 4 cut(s) 243, 514, 710, 854
BseGI GGATG 3 cut(s) 271, 470, 849
BseLI CCNNNNNNNGG 2 cut(s) 269, 832
BseMI GCAATG 1 cut(s) 643
BseMII CTCAG 1 cut(s) 500
BseNI ACTGG 1 cut(s) 883
BseSI GKGCMC 1 cut(s) 441
BseXI GCAGC 4 cut(s) 40, 68, 385, 625
BseYI CCCAGC 1 cut(s) 451
BshFI GGCC 5 cut(s) 185, 238, 558, 642, 861
BsiHKAI GWGCWC 1 cut(s) 331
BsiSI CCGG 1 cut(s) 263
BslI CCNNNNNNNGG 2 cut(s) 269, 832
BsmAI GTCTC 1 cut(s) 786
BsnI GGCC 5 cut(s) 185, 238, 558, 642, 861
Bsp1286I GDGCHC 3 cut(s) 297, 331, 441
Bsp143I GATC 2 cut(s) 286, 518
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 2 cut(s) 125, 733
BspANI GGCC 5 cut(s) 185, 238, 558, 642, 861
BspCNI CTCAG 1 cut(s) 501
BspLI GGNNCC 3 cut(s) 425, 492, 860
BspMAI CTGCAG 1 cut(s) 58
BspOI GCTAGC 1 cut(s) 620
BspPI GGATC 2 cut(s) 281, 526
BsrDI GCAATG 1 cut(s) 643
BsrI ACTGG 1 cut(s) 883
BssECI CCNNGG 4 cut(s) 243, 514, 710, 854
BssMI GATC 2 cut(s) 286, 518
BssT1I CCWWGG 3 cut(s) 243, 710, 854
Bst2UI CCWGG 1 cut(s) 428
Bst4CI ACNGT 1 cut(s) 538
Bst6I CTCTTC 1 cut(s) 713
BstC8I GCNNGC 2 cut(s) 240, 618
BstDEI CTNAG 1 cut(s) 509
BstDSI CCRYGG 2 cut(s) 243, 514
BstF5I GGATG 3 cut(s) 271, 470, 849
BstH2I RGCGCY 1 cut(s) 598
BstHHI GCGC 1 cut(s) 597
BstKTI GATC 2 cut(s) 289, 521
BstMAI GTCTC 1 cut(s) 786
BstMBI GATC 2 cut(s) 286, 518
BstMWI GCNNNNNNNGC 4 cut(s) 50, 235, 407, 416
BstNI CCWGG 1 cut(s) 428
BstNSI RCATGY 1 cut(s) 354
BstSCI CCNGG 1 cut(s) 426
BstSFI CTRYAG 2 cut(s) 54, 279
BstSLI GKGCMC 1 cut(s) 441
BstV1I GCAGC 4 cut(s) 40, 68, 385, 625
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BsuRI GGCC 5 cut(s) 185, 238, 558, 642, 861
BtgI CCRYGG 2 cut(s) 243, 514
BtrI CACGTC 1 cut(s) 667
BtsCI GGATG 3 cut(s) 271, 470, 849
BtsI GCAGTG 1 cut(s) 113
BtsIMutI CAGTG 3 cut(s) 113, 197, 410
Cac8I GCNNGC 2 cut(s) 240, 618
CfoI GCGC 1 cut(s) 597
Cfr13I GGNCC 2 cut(s) 111, 859
Csp6I GTAC 2 cut(s) 677, 823
CspCI CAANNNNNGTGG 2 cut(s) 727, 762
CviAII CATG 4 cut(s) 181, 244, 351, 892
CviQI GTAC 2 cut(s) 677, 823
DdeI CTNAG 1 cut(s) 509
DpnI GATC 2 cut(s) 288, 520
DpnII GATC 2 cut(s) 286, 518
DriI GACNNNNNGTC 1 cut(s) 778
EaeI YGGCCR 2 cut(s) 183, 640
Eam1104I CTCTTC 1 cut(s) 713
Eam1105I GACNNNNNGTC 1 cut(s) 778
EarI CTCTTC 1 cut(s) 713
Eco130I CCWWGG 3 cut(s) 243, 710, 854
Eco24I GRGCYC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 111
Eco47III AGCGCT 1 cut(s) 596
Eco57I CTGAAG 2 cut(s) 221, 434
EcoO109I RGGNCCY 1 cut(s) 859
EcoRI GAATTC 1 cut(s) 367
EcoRII CCWGG 1 cut(s) 426
EcoT14I CCWWGG 3 cut(s) 243, 710, 854
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 3 cut(s) 243, 710, 854
FaeI CATG 4 cut(s) 184, 247, 354, 895
FatI CATG 4 cut(s) 180, 243, 350, 891
FauNDI CATATG 1 cut(s) 403
Fnu4HI GCNGC 4 cut(s) 54, 57, 399, 614
FokI GGATG 3 cut(s) 278, 457, 856
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 4 cut(s) 54, 57, 399, 614
FspBI CTAG 4 cut(s) 24, 234, 617, 855
GlaI GCGC 1 cut(s) 596
GluI GCNGC 4 cut(s) 54, 57, 399, 614
GsaI CCCAGC 1 cut(s) 455
HaeII RGCGCY 1 cut(s) 598
HaeIII GGCC 5 cut(s) 185, 238, 558, 642, 861
HapII CCGG 1 cut(s) 263
HhaI GCGC 1 cut(s) 597
Hin1II CATG 4 cut(s) 184, 247, 354, 895
Hin6I GCGC 1 cut(s) 595
HinP1I GCGC 1 cut(s) 595
HincII GTYRAC 1 cut(s) 686
HindII GTYRAC 1 cut(s) 686
HpaII CCGG 1 cut(s) 263
HphI GGTGA 2 cut(s) 727, 893
Hpy166II GTNNAC 3 cut(s) 111, 321, 686
Hpy188I TCNGA 2 cut(s) 510, 763
Hpy188III TCNNGA 4 cut(s) 100, 290, 310, 670
Hpy8I GTNNAC 3 cut(s) 111, 321, 686
Hpy99I CGWCG 1 cut(s) 671
HpyAV CCTTC 3 cut(s) 386, 409, 667
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4IV ACGT 1 cut(s) 666
HpyCH4V TGCA 7 cut(s) 56, 146, 350, 401, 553, 738, 817
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 235, 407, 416
HpyF3I CTNAG 1 cut(s) 509
HpySE526I ACGT 1 cut(s) 666
Hsp92II CATG 4 cut(s) 184, 247, 354, 895
HspAI GCGC 1 cut(s) 595
Kzo9I GATC 2 cut(s) 286, 518
LmnI GCTCC 2 cut(s) 490, 583
Lsp1109I GCAGC 4 cut(s) 40, 68, 385, 625
LweI GCATC 1 cut(s) 133
MaeI CTAG 4 cut(s) 24, 234, 617, 855
MaeII ACGT 1 cut(s) 666
MaeIII GTNAC 2 cut(s) 340, 532
MalI GATC 2 cut(s) 288, 520
MboI GATC 2 cut(s) 286, 518
MboII GAAGA 7 cut(s) 12, 20, 40, 214, 230, 296, 730
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 3 cut(s) 297, 331, 441
MlsI TGGCCA 2 cut(s) 185, 642
MluCI AATT 7 cut(s) 14, 69, 154, 367, 654, 739, 907
MluNI TGGCCA 2 cut(s) 185, 642
MnlI CCTC 7 cut(s) 43, 96, 171, 187, 208, 262, 400
Mox20I TGGCCA 2 cut(s) 185, 642
MscI TGGCCA 2 cut(s) 185, 642
MseI TTAA 2 cut(s) 153, 906
Msp20I TGGCCA 2 cut(s) 185, 642
MspI CCGG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 428
MvaI CCWGG 1 cut(s) 428
MwoI GCNNNNNNNGC 4 cut(s) 50, 235, 407, 416
NcoI CCATGG 1 cut(s) 243
NdeI CATATG 1 cut(s) 403
NdeII GATC 2 cut(s) 286, 518
NheI GCTAGC 1 cut(s) 616
NlaIII CATG 4 cut(s) 184, 247, 354, 895
NlaIV GGNNCC 3 cut(s) 425, 492, 860
NspI RCATGY 1 cut(s) 354
PkrI GCNGC 4 cut(s) 55, 58, 400, 615
Psp6I CCWGG 1 cut(s) 426
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 1 cut(s) 426
PspN4I GGNNCC 3 cut(s) 425, 492, 860
PspPI GGNCC 2 cut(s) 111, 859
PstI CTGCAG 1 cut(s) 58
PsuI RGATCY 1 cut(s) 286
RsaI GTAC 2 cut(s) 678, 824
RsaNI GTAC 2 cut(s) 677, 823
SaqAI TTAA 2 cut(s) 153, 906
SatI GCNGC 4 cut(s) 54, 57, 399, 614
Sau3AI GATC 2 cut(s) 286, 518
Sau96I GGNCC 2 cut(s) 111, 859
ScrFI CCNGG 1 cut(s) 428
SduI GDGCHC 3 cut(s) 297, 331, 441
SfaNI GCATC 1 cut(s) 133
SfcI CTRYAG 2 cut(s) 54, 279
SinI GGWCC 1 cut(s) 111
Sse9I AATT 7 cut(s) 14, 69, 154, 367, 654, 739, 907
SsiI CCGC 2 cut(s) 125, 733
SspMI CTAG 4 cut(s) 24, 234, 617, 855
StyD4I CCNGG 1 cut(s) 426
StyI CCWWGG 3 cut(s) 243, 710, 854
TaaI ACNGT 1 cut(s) 538
TaiI ACGT 1 cut(s) 669
TaqI TCGA 2 cut(s) 99, 669
TasI AATT 7 cut(s) 14, 69, 154, 367, 654, 739, 907
Tru1I TTAA 2 cut(s) 153, 906
Tru9I TTAA 2 cut(s) 153, 906
TscAI CASTG 3 cut(s) 113, 204, 417
TseI GCWGC 4 cut(s) 53, 56, 398, 613
TspDTI ATGAA 9 cut(s) 17, 21, 47, 51, 270, 380, 558, 717, 860
TspGWI ACGGA 2 cut(s) 155, 807
TspRI CASTG 3 cut(s) 113, 204, 417
VpaK11BI GGWCC 1 cut(s) 111
XapI RAATTY 4 cut(s) 14, 69, 367, 654
XceI RCATGY 1 cut(s) 354
XmaJI CCTAGG 1 cut(s) 854
XspI CTAG 4 cut(s) 24, 234, 617, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.