FvH4_2g26890

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000885
Physical Location & Seq
Forward (+)
21511337 .. 21514025
2689 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26890.t3

Sequence Viewer

Length: 1311 bp
ATGGACAGACCAGAAGCATCTCCGTCTTGTCGTGACGTCGTTCTGAGTTTTAGGGGTTCCAGCAAATTCCCAGACCAGTTACTTACTGCATTAGTCGAAAGAGGAATACATGCTTTCAAGGATGACCCAGAACTTAAACGTGGTTCAGAAATTTCTACAGCCGTTTGCGATTCAAGAGTTTCTGTCGTTCTCTTGTCAGAAAAGTATGCAAACTCGACGTGGTGTTTAGAAGAACTTGCATGTATTCTCAAACACAAGCTGGATGTGATGCCAATTTTCTACGGTGTTGATCCCTCCGATGTACGAAACCAAACAGGGAGACTTGCAGCACAGTTTAAGATATTTGAAGACAAATATAAGGATGAACCAGAGAAGGTGATAAGGTGGAAAGCTGCTCTTACTGAAGTGGCAAATCTCTCTGGCTGGAATTCAAAGGGCCGGTGTGAATCAGATCTGATCAAAGATATTGTCAAAGATGTATGGCAAAAATTGCATCCTGGATTGCCCTTTCACTCACCTTCACAATTGGCGGTTAATAAGATTGGCTCCACACGATATTCTTCTTCTTCTTCCTCTTCTCATCAAAAAAAGAAGTGTGATGTCTTTATAAGTTTCAGAGGAAAGGATACCCGCTCGAATTTTTTCAGCCATCTATACAATGCCTTGGTTGACTACGGAATTTCCACGTATAGAGATGACATACATCTTGAAAGAGGAAAATCCATTCCCTCAGAACTTGTGAAAGCAATTAACGAAGCAAGGATTTGTCTCGTCATTTTTTCACCCAACTTCACTTCTTCAACATGGTGCTTGGATGAACTCTCCCAAATTCTTCAAAAACGAAACAGAGTATCTGTGGTGCGGAATGGAACTCTGGAGTCATTTGGAGAACATGAAGATGTTTGTTTGAGCAATAGAGAAAAAGTGGAGAAGTTGATGCCTACCCTTAGACATGCAGCCATGTTTAACAGAGGAGCCAGCTGCCAACTGACCAAACTTAGACTGTTGGTTAACTACAACGGGATGTGGACTAATGCCGGATACATTGGTGGCAAAACAAAAGGTATACTTGTTTCAGAGGACATTACATATCAGGAACTTGAGGACCGAGTTTATCGCATTGTCGGGGTAGATCCAAGAGAATATAAGATGATAATGAAGGCCAAATATGAATCAAAAGATCCCACTCAACCTGTAGAGATAATCGACGACGAAGATCTTGGATTTTTCATTTTAGAAAGTCTTTCAGATGAGCGCTGCAGAATTCCATTGTGCATAACTCTAGAACCACATAAGGATGATCCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

437

Amino Acids

49.61

Weight (kDa)

7.11

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR_2 PF13676 13 - 98 8e-08 TIR domain
TIR PF01582 21 - 168 2.9e-36 TIR domain
TIR PF01582 200 - 282 1.1e-24 TIR domain
TIR_2 PF13676 201 - 288 1.2e-14 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 608
AatII GACGTC 1 cut(s) 39
AccBSI CCGCTC 1 cut(s) 633
AccI GTMKAC 1 cut(s) 1066
AciI CCGC 3 cut(s) 530, 631, 862
AclWI GGATC 4 cut(s) 284, 1127, 1175, 1295
AcsI RAATTY 7 cut(s) 65, 150, 427, 637, 678, 828, 1263
AcuI CTGAAG 1 cut(s) 423
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 1 cut(s) 303
AfeI AGCGCT 1 cut(s) 1256
AgsI TTSAA 7 cut(s) 118, 174, 347, 432, 710, 801, 836
AjiI CACGTC 1 cut(s) 219
AjnI CCWGG 1 cut(s) 496
AluBI AGCT 3 cut(s) 259, 392, 981
AluI AGCT 3 cut(s) 259, 392, 981
Alw26I GTCTC 2 cut(s) 313, 773
AlwI GGATC 4 cut(s) 284, 1127, 1175, 1295
Aor51HI AGCGCT 1 cut(s) 1256
AoxI GGCC 2 cut(s) 436, 1161
ApeKI GCWGC 5 cut(s) 326, 392, 956, 981, 1257
ApoI RAATTY 7 cut(s) 65, 150, 427, 637, 678, 828, 1263
ArsI GACNNNNNNTTYG 2 cut(s) 453, 485
Asp700I GAANNNNTTC 1 cut(s) 641
AspLEI GCGC 1 cut(s) 1257
AspS9I GGNCC 2 cut(s) 436, 1105
AsuHPI GGTGA 3 cut(s) 388, 507, 774
AvaII GGWCC 1 cut(s) 1105
BaeI ACNNNNGTAYC 2 cut(s) 1033, 1066
BbsI GAAGAC 1 cut(s) 354
BbvI GCAGC 5 cut(s) 338, 379, 968, 968, 1244
BccI CCATC 1 cut(s) 657
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 498
BciVI GTATCC 2 cut(s) 619, 1034
BclI TGATCA 1 cut(s) 456
BcoDI GTCTC 2 cut(s) 313, 773
BfaI CTAG 1 cut(s) 1283
BfmI CTRYAG 3 cut(s) 156, 1194, 1258
BfoI RGCGCY 1 cut(s) 1258
BfuI GTATCC 2 cut(s) 619, 1034
BglII AGATCT 2 cut(s) 451, 1216
BisI GCNGC 5 cut(s) 327, 393, 957, 982, 1258
BlsI GCNGC 5 cut(s) 328, 394, 958, 983, 1259
Bme1390I CCNGG 1 cut(s) 498
Bme18I GGWCC 1 cut(s) 1105
BmgBI CACGTC 1 cut(s) 219
BmgT120I GGNCC 2 cut(s) 436, 1105
BmiI GGNNCC 3 cut(s) 58, 547, 976
BmrFI CCNGG 1 cut(s) 498
BmsI GCATC 4 cut(s) 26, 258, 502, 927
BpiI GAAGAC 1 cut(s) 354
BpmI CTGGAG 1 cut(s) 896
BpuEI CTTGAG 1 cut(s) 1121
BsaAI YACGTR 1 cut(s) 687
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 663
Bse118I RCCGGY 1 cut(s) 438
Bse1I ACTGG 1 cut(s) 76
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 1 cut(s) 663
BseGI GGATG 7 cut(s) 127, 268, 367, 493, 820, 1029, 1303
BseMII CTCAG 2 cut(s) 35, 744
BseNI ACTGG 1 cut(s) 76
BseRI GAGGAG 1 cut(s) 987
BseXI GCAGC 5 cut(s) 338, 379, 968, 968, 1244
BshFI GGCC 2 cut(s) 438, 1163
BsiSI CCGG 2 cut(s) 439, 1038
BsmAI GTCTC 2 cut(s) 313, 773
BsnI GGCC 2 cut(s) 438, 1163
Bsp143I GATC 7 cut(s) 289, 451, 456, 1132, 1180, 1216, 1300
BspACI CCGC 3 cut(s) 530, 631, 862
BspANI GGCC 2 cut(s) 438, 1163
BspCNI CTCAG 2 cut(s) 36, 743
BspLI GGNNCC 3 cut(s) 58, 547, 976
BspMAI CTGCAG 1 cut(s) 1262
BspPI GGATC 4 cut(s) 284, 1127, 1175, 1295
BsrBI CCGCTC 1 cut(s) 633
BsrFI RCCGGY 1 cut(s) 438
BsrI ACTGG 1 cut(s) 76
BssAI RCCGGY 1 cut(s) 438
BssECI CCNNGG 1 cut(s) 663
BssMI GATC 7 cut(s) 289, 451, 456, 1132, 1180, 1216, 1300
BssNAI GTATAC 1 cut(s) 1067
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 663
Bst1107I GTATAC 1 cut(s) 1067
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 3 cut(s) 284, 333, 1005
Bst6I CTCTTC 1 cut(s) 580
BstACI GRCGYC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 490
BstBAI YACGTR 1 cut(s) 687
BstC8I GCNNGC 1 cut(s) 979
BstDEI CTNAG 4 cut(s) 44, 730, 947, 998
BstF5I GGATG 7 cut(s) 127, 268, 367, 493, 820, 1029, 1303
BstH2I RGCGCY 1 cut(s) 1258
BstHHI GCGC 1 cut(s) 1257
BstKTI GATC 7 cut(s) 292, 454, 459, 1135, 1183, 1219, 1303
BstMAI GTCTC 2 cut(s) 313, 773
BstMBI GATC 7 cut(s) 289, 451, 456, 1132, 1180, 1216, 1300
BstMWI GCNNNNNNNGC 1 cut(s) 490
BstNI CCWGG 1 cut(s) 498
BstNSI RCATGY 3 cut(s) 113, 243, 956
BstSCI CCNGG 1 cut(s) 496
BstSFI CTRYAG 3 cut(s) 156, 1194, 1258
BstV1I GCAGC 5 cut(s) 338, 379, 968, 968, 1244
BstV2I GAAGAC 1 cut(s) 354
BstX2I RGATCY 4 cut(s) 451, 1132, 1180, 1216
BstYI RGATCY 4 cut(s) 451, 1132, 1180, 1216
BstZ17I GTATAC 1 cut(s) 1067
BsuI GTATCC 2 cut(s) 619, 1034
BsuRI GGCC 2 cut(s) 438, 1163
BtrI CACGTC 1 cut(s) 219
BtsCI GGATG 7 cut(s) 127, 268, 367, 493, 820, 1029, 1303
Cac8I GCNNGC 1 cut(s) 979
CfoI GCGC 1 cut(s) 1257
Cfr10I RCCGGY 1 cut(s) 438
Cfr13I GGNCC 2 cut(s) 436, 1105
Csp6I GTAC 1 cut(s) 302
CviAII CATG 6 cut(s) 110, 240, 804, 893, 953, 961
CviQI GTAC 1 cut(s) 302
DdeI CTNAG 4 cut(s) 44, 730, 947, 998
DpnI GATC 7 cut(s) 291, 453, 458, 1134, 1182, 1218, 1302
DpnII GATC 7 cut(s) 289, 451, 456, 1132, 1180, 1216, 1300
Eam1104I CTCTTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 580
Eco130I CCWWGG 1 cut(s) 663
Eco47I GGWCC 1 cut(s) 1105
Eco47III AGCGCT 1 cut(s) 1256
Eco57I CTGAAG 1 cut(s) 423
EcoRI GAATTC 2 cut(s) 427, 1263
EcoRII CCWGG 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 6 cut(s) 113, 243, 807, 896, 956, 964
FalI AAGNNNNNCTT 4 cut(s) 381, 413, 1053, 1085
FatI CATG 6 cut(s) 109, 239, 803, 892, 952, 960
FauI CCCGC 1 cut(s) 638
FbaI TGATCA 1 cut(s) 456
FblI GTMKAC 1 cut(s) 1066
Fnu4HI GCNGC 5 cut(s) 327, 393, 957, 982, 1258
FokI GGATG 6 cut(s) 134, 275, 374, 480, 827, 1036
Fsp4HI GCNGC 5 cut(s) 327, 393, 957, 982, 1258
FspBI CTAG 1 cut(s) 1283
GlaI GCGC 1 cut(s) 1256
GluI GCNGC 5 cut(s) 327, 393, 957, 982, 1258
GsuI CTGGAG 1 cut(s) 896
HaeII RGCGCY 1 cut(s) 1258
HaeIII GGCC 2 cut(s) 438, 1163
HapII CCGG 2 cut(s) 439, 1038
HhaI GCGC 1 cut(s) 1257
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 6 cut(s) 113, 243, 807, 896, 956, 964
Hin6I GCGC 1 cut(s) 1255
HinP1I GCGC 1 cut(s) 1255
HincII GTYRAC 2 cut(s) 670, 1012
HindII GTYRAC 2 cut(s) 670, 1012
HinfI GANTC 4 cut(s) 170, 446, 878, 1172
HpaI GTTAAC 1 cut(s) 1012
HpaII CCGG 2 cut(s) 439, 1038
HphI GGTGA 3 cut(s) 388, 507, 774
Hpy166II GTNNAC 4 cut(s) 670, 1012, 1029, 1067
Hpy188III TCNNGA 6 cut(s) 32, 174, 707, 875, 1094, 1283
Hpy8I GTNNAC 4 cut(s) 670, 1012, 1029, 1067
Hpy99I CGWCG 4 cut(s) 41, 220, 1211, 1214
HpyAV CCTTC 3 cut(s) 367, 528, 1153
HpyCH4III ACNGT 3 cut(s) 284, 333, 1005
HpyCH4IV ACGT 4 cut(s) 36, 139, 218, 686
HpyCH4V TGCA 8 cut(s) 89, 209, 239, 326, 493, 956, 1260, 1275
HpyF10VI GCNNNNNNNGC 1 cut(s) 490
HpyF3I CTNAG 4 cut(s) 44, 730, 947, 998
HpySE526I ACGT 4 cut(s) 36, 139, 218, 686
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 6 cut(s) 113, 243, 807, 896, 956, 964
HspAI GCGC 1 cut(s) 1255
Ksp22I TGATCA 1 cut(s) 456
KspAI GTTAAC 1 cut(s) 1012
Kzo9I GATC 7 cut(s) 289, 451, 456, 1132, 1180, 1216, 1300
LmnI GCTCC 2 cut(s) 551, 974
Lsp1109I GCAGC 5 cut(s) 338, 379, 968, 968, 1244
LweI GCATC 4 cut(s) 26, 258, 502, 927
MaeI CTAG 1 cut(s) 1283
MaeII ACGT 4 cut(s) 36, 139, 218, 686
MaeIII GTNAC 2 cut(s) 32, 78
MalI GATC 7 cut(s) 291, 453, 458, 1134, 1182, 1218, 1302
MbiI CCGCTC 1 cut(s) 633
MboI GATC 7 cut(s) 289, 451, 456, 1132, 1180, 1216, 1300
MfeI CAATTG 1 cut(s) 524
MflI RGATCY 4 cut(s) 451, 1132, 1180, 1216
MlyI GAGTC 1 cut(s) 887
MnlI CCTC 9 cut(s) 95, 304, 583, 611, 707, 739, 965, 1072, 1096
MroXI GAANNNNTTC 1 cut(s) 641
MseI TTAA 6 cut(s) 135, 336, 534, 750, 966, 1011
MslI CAYNNNNRTG 2 cut(s) 897, 1296
MspA1I CMGCKG 1 cut(s) 981
MspI CCGG 2 cut(s) 439, 1038
MspR9I CCNGG 1 cut(s) 498
MunI CAATTG 1 cut(s) 524
MvaI CCWGG 1 cut(s) 498
MwoI GCNNNNNNNGC 1 cut(s) 490
NdeII GATC 7 cut(s) 289, 451, 456, 1132, 1180, 1216, 1300
NlaIII CATG 6 cut(s) 113, 243, 807, 896, 956, 964
NlaIV GGNNCC 3 cut(s) 58, 547, 976
NmuCI GTSAC 1 cut(s) 32
NspI RCATGY 3 cut(s) 113, 243, 956
PdmI GAANNNNTTC 1 cut(s) 641
PfeI GAWTC 3 cut(s) 170, 446, 1172
PfoI TCCNGGA 1 cut(s) 496
PkrI GCNGC 5 cut(s) 328, 394, 958, 983, 1259
PleI GAGTC 1 cut(s) 886
PpsI GAGTC 1 cut(s) 886
Ppu21I YACGTR 1 cut(s) 687
PsiI TTATAA 1 cut(s) 608
Psp6I CCWGG 1 cut(s) 496
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 3 cut(s) 58, 547, 976
PspPI GGNCC 2 cut(s) 436, 1105
PstI CTGCAG 1 cut(s) 1262
PsuI RGATCY 4 cut(s) 451, 1132, 1180, 1216
PvuII CAGCTG 1 cut(s) 981
RsaI GTAC 1 cut(s) 303
RsaNI GTAC 1 cut(s) 302
RseI CAYNNNNRTG 2 cut(s) 897, 1296
SaqAI TTAA 6 cut(s) 135, 336, 534, 750, 966, 1011
SatI GCNGC 5 cut(s) 327, 393, 957, 982, 1258
Sau3AI GATC 7 cut(s) 289, 451, 456, 1132, 1180, 1216, 1300
Sau96I GGNCC 2 cut(s) 436, 1105
SchI GAGTC 1 cut(s) 887
ScrFI CCNGG 1 cut(s) 498
SfaNI GCATC 4 cut(s) 26, 258, 502, 927
SfcI CTRYAG 3 cut(s) 156, 1194, 1258
SinI GGWCC 1 cut(s) 1105
SmiMI CAYNNNNRTG 2 cut(s) 897, 1296
SmlI CTYRAG 1 cut(s) 1100
SmoI CTYRAG 1 cut(s) 1100
SsiI CCGC 3 cut(s) 530, 631, 862
SspMI CTAG 1 cut(s) 1283
StyD4I CCNGG 1 cut(s) 496
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 3 cut(s) 284, 333, 1005
TaiI ACGT 4 cut(s) 39, 142, 221, 689
TaqI TCGA 4 cut(s) 96, 215, 635, 1206
TaqII GACCGA 1 cut(s) 1122
TfiI GAWTC 3 cut(s) 170, 446, 1172
Tru1I TTAA 6 cut(s) 135, 336, 534, 750, 966, 1011
Tru9I TTAA 6 cut(s) 135, 336, 534, 750, 966, 1011
TseFI GTSAC 1 cut(s) 32
TseI GCWGC 5 cut(s) 326, 392, 956, 981, 1257
Tsp45I GTSAC 1 cut(s) 32
TspDTI ATGAA 6 cut(s) 378, 831, 909, 1172, 1185, 1219
TspGWI ACGGA 2 cut(s) 12, 690
VpaK11BI GGWCC 1 cut(s) 1105
XapI RAATTY 7 cut(s) 65, 150, 427, 637, 678, 828, 1263
XbaI TCTAGA 1 cut(s) 1282
XceI RCATGY 3 cut(s) 113, 243, 956
XmiI GTMKAC 1 cut(s) 1066
XmnI GAANNNNTTC 1 cut(s) 641
XspI CTAG 1 cut(s) 1283
ZraI GACGTC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.