Rorug05G0146700

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000885
Physical Location & Seq
Forward (+)
13606540 .. 13612352
5813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0146700.1

Sequence Viewer

Length: 1524 bp
ATGGAAGCACCCGGCAGCCGTAGCAATACCAACAAAGACCAAATCGTCGACGTCCGGTCGGTGGTCGAAGCTGTCTCCGCCGACGACTCCTCCGACGCCCCTCTTTACAAAGTCGAAAGCCTCTGTATGCGTTGCGGCGAAAACGGCACAACCAGCTTTTTGTTCACTGTGATTCCAAACTTCAGAAAGATTTTGCTCTCCGCTTTCGAGTGTCCGCATTGCAATGAGAGGAATAATGAGGTTCAGTTTGCTGGTGAGCTTCAACCCAAGGGTTGTACCTACCGCTTGGAGGTGCCTTCCGGGGATCCCAAGATGCTCAATAGGCAAGTGGTGAAATCTGAGTTTGCTACTATCAAGATTCCTGAATTAGATTTTGAGATTCCTCCAGAGTCTCAGCGTGGATCTTTGTCAACGGTGGAAGGGATATTATTACGAGCAGCGGATGAGTTGCAGGCACTTCAAGAAGAACGCAAGAGAGTGGATCCTCACACTGCTGAAGCTATAGACCAATTTTTGTTAAAGCTGAGAGCATGTGCAACAGGAGATTCGTCTTTCACCTTCGTTTTGGATGATCCTTCTGGAAACAGCTTCATTGAGAACCTGTTTGCTCCATCCCCCGATCCATCACTAAGTATCAAGTTTTATGAGCGAACTCCAGAGCAACAATCAGTGCTAGGGTATGTTGTTGACTCATCACAAACAGGACATGCTCATGATGTAGCATCAACGAATCCAATTGGTCCTGACCAGGAGCAAAGAGTACCACATGGTTCAATTGGTGCAGCAGCTGGTCATCGTGCCATTGCTCAGAGTAATACTGAGGAAATTTCTGAAGCTTTGTTTCGATACTCTGCACCAGAGGAGGTGATGACTTTCCCATCAACATGCGGAACTTGTACTGCAAAATGTGAGACTCGAATGTTTGTCACCCACATCCCATACTTCCAACAAGTAATTGTCATGGCATCCACTTGTGACGCTTGTGGTTACCGCAACTCTGAGTTGAAGCCTGGTGGAAGAATTCCTGATAAAGGGAAGAAAATTACTCTTTGCGTAAAGAATGTTAAGGATCTTAGTCGTGATGTCATAAAGTCGGATACTGCAAGTGTGAAAATTCCGGAGCTTGAGTTGGAGCTGGGTAGTGGGACCCTCGGAGGACTTGTTACAACTATCGAAGGGTTGATCACGAAAATTTGTGAAAGTCTGGAGAGAGTGCATGGATTTTCTTTCGGAGATAGTCATGATCAAAATAAGAAAAGCAAGTGGTTAAACTTTACCGCAAGCCTAAATAAGCTTCTGAGTTTAGAAGAGCCTTTTACTTTAATTCTTGATGACGCATTGGCTAACTCTTTCATTGCACCCGTGACTGATGATATGAAAGACGACCACGAGCTAATGTTTGAGGAGTATGAGAGGTCATGGGAGCAGAACGAGGAACTGGGTCTGAATGACATTGATACCTCTTCCGCTGATGCTGCATATATCACTGAGGAGATGGCGGCAACAGACAAGACAGAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

507

Amino Acids

55.82

Weight (kDa)

4.69

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_ZPR1 PF03367 38 - 89 1.9e-19 ZPR1 zinc-finger domain
jr-ZPR1 PF22794 105 - 226 1.5e-43 ZPR1 jelly-roll domain
Zn_ribbon_ZPR1 PF03367 288 - 342 6.6e-25 ZPR1 zinc-finger domain
jr-ZPR1 PF22794 356 - 482 1.6e-38 ZPR1 jelly-roll domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 44
AatII GACGTC 1 cut(s) 54
AccB1I GGYRCC 1 cut(s) 292
AccI GTMKAC 1 cut(s) 48
AccIII TCCGGA 1 cut(s) 1117
AclWI GGATC 8 cut(s) 299, 312, 409, 476, 489, 566, 614, 1077
AcsI RAATTY 4 cut(s) 825, 1020, 1113, 1191
AcuI CTGAAG 3 cut(s) 166, 516, 852
AcyI GRCGYC 2 cut(s) 51, 96
AfaI GTAC 3 cut(s) 277, 762, 898
AfiI CCNNNNNNNGG 3 cut(s) 61, 289, 1031
AgsI TTSAA 4 cut(s) 263, 461, 774, 1006
AhdI GACNNNNNGTC 1 cut(s) 55
AjnI CCWGG 2 cut(s) 747, 1009
Alw26I GTCTC 3 cut(s) 79, 396, 905
AlwI GGATC 8 cut(s) 299, 312, 409, 476, 489, 566, 614, 1077
AlwNI CAGNNNCTG 1 cut(s) 788
Aor13HI TCCGGA 1 cut(s) 1117
ApeKI GCWGC 5 cut(s) 15, 437, 782, 785, 1475
ApoI RAATTY 4 cut(s) 825, 1020, 1113, 1191
AspS9I GGNCC 2 cut(s) 740, 1146
AsuC2I CCSGG 2 cut(s) 12, 301
AsuHPI GGTGA 5 cut(s) 266, 343, 547, 877, 919
AvaII GGWCC 2 cut(s) 740, 1146
BamHI GGATCC 2 cut(s) 304, 481
BanI GGYRCC 1 cut(s) 292
BauI CACGAG 1 cut(s) 1388
BbvI GCAGC 5 cut(s) 27, 449, 794, 797, 1462
BccI CCATC 4 cut(s) 619, 631, 886, 1489
BceAI ACGGC 2 cut(s) 3, 160
BciT130I CCWGG 2 cut(s) 749, 1011
BciVI GTATCC 1 cut(s) 1090
BclI TGATCA 2 cut(s) 1182, 1243
BcnI CCSGG 2 cut(s) 12, 301
BcoDI GTCTC 3 cut(s) 79, 396, 905
BfaI CTAG 1 cut(s) 674
BfmI CTRYAG 1 cut(s) 501
BfuI GTATCC 1 cut(s) 1090
BisI GCNGC 7 cut(s) 16, 136, 438, 783, 786, 1476, 1500
BlsI GCNGC 7 cut(s) 17, 137, 439, 784, 787, 1477, 1501
Bme1390I CCNGG 4 cut(s) 12, 301, 749, 1011
Bme18I GGWCC 2 cut(s) 740, 1146
BmeRI GACNNNNNGTC 1 cut(s) 55
BmgT120I GGNCC 2 cut(s) 740, 1146
BmiI GGNNCC 5 cut(s) 294, 306, 483, 1147, 1148
BmrFI CCNGG 4 cut(s) 12, 301, 749, 1011
BmrI ACTGGG 1 cut(s) 1448
BmsI GCATC 4 cut(s) 303, 731, 974, 1462
BmuI ACTGGG 1 cut(s) 1448
BpmI CTGGAG 3 cut(s) 369, 639, 1226
BpuEI CTTGAG 1 cut(s) 1145
BpuMI CCSGG 2 cut(s) 12, 301
BsaHI GRCGYC 2 cut(s) 51, 96
BsaJI CCNNGG 3 cut(s) 267, 300, 1150
BsaWI WCCGGW 2 cut(s) 54, 1117
BsaXI ACNNNNNCTCC 6 cut(s) 74, 104, 1112, 1142, 1146, 1176
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 289, 1031
Bse1I ACTGG 1 cut(s) 1443
Bse3DI GCAATG 4 cut(s) 217, 229, 801, 1353
BseAI TCCGGA 1 cut(s) 1117
BseBI CCWGG 2 cut(s) 749, 1011
BseDI CCNNGG 3 cut(s) 267, 300, 1150
BseGI GGATG 5 cut(s) 448, 574, 611, 933, 965
BseLI CCNNNNNNNGG 3 cut(s) 61, 289, 1031
BseMI GCAATG 4 cut(s) 217, 229, 801, 1353
BseMII CTCAG 8 cut(s) 330, 407, 515, 810, 821, 990, 1289, 1479
BseNI ACTGG 1 cut(s) 1443
BseRI GAGGAG 4 cut(s) 79, 875, 1418, 1505
BseXI GCAGC 5 cut(s) 27, 449, 794, 797, 1462
BseYI CCCAGC 1 cut(s) 1135
BsgI GTGCAG 2 cut(s) 801, 837
Bsh1285I CGRYCG 1 cut(s) 59
BshNI GGYRCC 1 cut(s) 292
BsiEI CGRYCG 1 cut(s) 59
BsiSI CCGG 4 cut(s) 12, 55, 300, 1118
BslFI GGGAC 1 cut(s) 1159
BslI CCNNNNNNNGG 3 cut(s) 61, 289, 1031
BsmAI GTCTC 3 cut(s) 79, 396, 905
BsmFI GGGAC 1 cut(s) 1159
Bsp13I TCCGGA 1 cut(s) 1117
Bsp143I GATC 8 cut(s) 304, 401, 481, 571, 619, 1069, 1182, 1243
BspCNI CTCAG 8 cut(s) 331, 406, 516, 811, 820, 991, 1290, 1480
BspEI TCCGGA 1 cut(s) 1117
BspHI TCATGA 2 cut(s) 712, 1240
BspLI GGNNCC 5 cut(s) 294, 306, 483, 1147, 1148
BspPI GGATC 8 cut(s) 299, 312, 409, 476, 489, 566, 614, 1077
BspQI GCTCTTC 1 cut(s) 1302
BspT107I GGYRCC 1 cut(s) 292
BsrDI GCAATG 4 cut(s) 217, 229, 801, 1353
BsrI ACTGG 1 cut(s) 1443
BssECI CCNNGG 3 cut(s) 267, 300, 1150
BssMI GATC 8 cut(s) 304, 401, 481, 571, 619, 1069, 1182, 1243
BssNI GRCGYC 2 cut(s) 51, 96
BssSI CACGAG 1 cut(s) 1388
BssT1I CCWWGG 1 cut(s) 267
Bst2BI CACGAG 1 cut(s) 1388
Bst2UI CCWGG 2 cut(s) 749, 1011
Bst4CI ACNGT 2 cut(s) 169, 415
Bst6I CTCTTC 2 cut(s) 1302, 1468
BstACI GRCGYC 2 cut(s) 51, 96
BstC8I GCNNGC 2 cut(s) 453, 1282
BstEII GGTNACC 1 cut(s) 986
BstENI CCTNNNNNAGG 1 cut(s) 1029
BstF5I GGATG 5 cut(s) 448, 574, 611, 933, 965
BstKTI GATC 8 cut(s) 307, 404, 484, 574, 622, 1072, 1185, 1246
BstMAI GTCTC 3 cut(s) 79, 396, 905
BstMBI GATC 8 cut(s) 304, 401, 481, 571, 619, 1069, 1182, 1243
BstMCI CGRYCG 1 cut(s) 59
BstMWI GCNNNNNNNGC 6 cut(s) 21, 77, 144, 153, 322, 1475
BstNI CCWGG 2 cut(s) 749, 1011
BstNSI RCATGY 3 cut(s) 534, 710, 888
BstPI GGTNACC 1 cut(s) 986
BstSCI CCNGG 4 cut(s) 10, 299, 747, 1009
BstSFI CTRYAG 1 cut(s) 501
BstV1I GCAGC 5 cut(s) 27, 449, 794, 797, 1462
BstX2I RGATCY 4 cut(s) 304, 401, 481, 1069
BstYI RGATCY 4 cut(s) 304, 401, 481, 1069
BsuI GTATCC 1 cut(s) 1090
BtsCI GGATG 5 cut(s) 448, 574, 611, 933, 965
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 4 cut(s) 165, 489, 675, 1485
Cac8I GCNNGC 2 cut(s) 453, 1282
CaiI CAGNNNCTG 1 cut(s) 788
CciI TCATGA 2 cut(s) 712, 1240
Cfr13I GGNCC 2 cut(s) 740, 1146
CseI GACGC 3 cut(s) 104, 986, 1343
Csp6I GTAC 3 cut(s) 276, 761, 897
CviAII CATG 9 cut(s) 531, 707, 713, 767, 885, 961, 1217, 1241, 1419
CviQI GTAC 3 cut(s) 276, 761, 897
DpnI GATC 8 cut(s) 306, 403, 483, 573, 621, 1071, 1184, 1245
DpnII GATC 8 cut(s) 304, 401, 481, 571, 619, 1069, 1182, 1243
DrdI GACNNNNNNGTC 1 cut(s) 44
DriI GACNNNNNGTC 1 cut(s) 55
DseDI GACNNNNNNGTC 1 cut(s) 44
Eam1104I CTCTTC 2 cut(s) 1302, 1468
Eam1105I GACNNNNNGTC 1 cut(s) 55
EarI CTCTTC 2 cut(s) 1302, 1468
EciI GGCGGA 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 267
Eco47I GGWCC 2 cut(s) 740, 1146
Eco57I CTGAAG 3 cut(s) 166, 516, 852
Eco91I GGTNACC 1 cut(s) 986
EcoNI CCTNNNNNAGG 1 cut(s) 1029
EcoO109I RGGNCCY 1 cut(s) 1146
EcoO65I GGTNACC 1 cut(s) 986
EcoRI GAATTC 1 cut(s) 1020
EcoRII CCWGG 2 cut(s) 747, 1009
EcoT14I CCWWGG 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 9 cut(s) 534, 710, 716, 770, 888, 964, 1220, 1244, 1422
FaqI GGGAC 1 cut(s) 1159
FatI CATG 9 cut(s) 530, 706, 712, 766, 884, 960, 1216, 1240, 1418
FbaI TGATCA 2 cut(s) 1182, 1243
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 7 cut(s) 16, 136, 438, 783, 786, 1476, 1500
FokI GGATG 5 cut(s) 455, 581, 598, 920, 952
Fsp4HI GCNGC 7 cut(s) 16, 136, 438, 783, 786, 1476, 1500
FspBI CTAG 1 cut(s) 674
GluI GCNGC 7 cut(s) 16, 136, 438, 783, 786, 1476, 1500
GsaI CCCAGC 1 cut(s) 1139
GsuI CTGGAG 3 cut(s) 369, 639, 1226
HapII CCGG 4 cut(s) 12, 55, 300, 1118
HgaI GACGC 3 cut(s) 104, 986, 1343
Hin1I GRCGYC 2 cut(s) 51, 96
Hin1II CATG 9 cut(s) 534, 710, 716, 770, 888, 964, 1220, 1244, 1422
HincII GTYRAC 3 cut(s) 49, 411, 688
HindII GTYRAC 3 cut(s) 49, 411, 688
HindIII AAGCTT 2 cut(s) 834, 1292
HinfI GANTC 9 cut(s) 86, 172, 358, 379, 389, 545, 689, 730, 913
HpaII CCGG 4 cut(s) 12, 55, 300, 1118
HphI GGTGA 5 cut(s) 266, 343, 547, 877, 919
Hpy166II GTNNAC 4 cut(s) 49, 165, 411, 688
Hpy8I GTNNAC 4 cut(s) 49, 165, 411, 688
Hpy99I CGWCG 4 cut(s) 50, 53, 86, 98
HpyAV CCTTC 5 cut(s) 306, 413, 568, 585, 1169
HpyCH4III ACNGT 2 cut(s) 169, 415
HpyCH4IV ACGT 1 cut(s) 51
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 77, 144, 153, 322, 1475
HpySE526I ACGT 1 cut(s) 51
Hsp92I GRCGYC 2 cut(s) 51, 96
Hsp92II CATG 9 cut(s) 534, 710, 716, 770, 888, 964, 1220, 1244, 1422
KflI GGGWCCC 1 cut(s) 1146
Kpn2I TCCGGA 1 cut(s) 1117
Ksp22I TGATCA 2 cut(s) 1182, 1243
Kzo9I GATC 8 cut(s) 304, 401, 481, 571, 619, 1069, 1182, 1243
LguI GCTCTTC 1 cut(s) 1302
LmnI GCTCC 5 cut(s) 613, 751, 1120, 1132, 1423
Lsp1109I GCAGC 5 cut(s) 27, 449, 794, 797, 1462
LweI GCATC 4 cut(s) 303, 731, 974, 1462
MaeI CTAG 1 cut(s) 674
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 5 cut(s) 925, 974, 986, 1162, 1363
MalI GATC 8 cut(s) 306, 403, 483, 573, 621, 1071, 1184, 1245
MboI GATC 8 cut(s) 304, 401, 481, 571, 619, 1069, 1182, 1243
MboII GAAGA 5 cut(s) 476, 1029, 1048, 1319, 1455
MfeI CAATTG 2 cut(s) 735, 774
MflI RGATCY 4 cut(s) 304, 401, 481, 1069
MlyI GAGTC 4 cut(s) 80, 398, 683, 907
MmeI TCCRAC 4 cut(s) 117, 970, 1074, 1110
MroI TCCGGA 1 cut(s) 1117
MseI TTAA 4 cut(s) 518, 1065, 1268, 1322
MslI CAYNNNNRTG 3 cut(s) 222, 711, 883
MspA1I CMGCKG 3 cut(s) 440, 788, 1469
MspI CCGG 4 cut(s) 12, 55, 300, 1118
MspR9I CCNGG 4 cut(s) 12, 301, 749, 1011
MunI CAATTG 2 cut(s) 735, 774
MvaI CCWGG 2 cut(s) 749, 1011
MwoI GCNNNNNNNGC 6 cut(s) 21, 77, 144, 153, 322, 1475
NciI CCSGG 2 cut(s) 12, 301
NdeII GATC 8 cut(s) 304, 401, 481, 571, 619, 1069, 1182, 1243
NlaIII CATG 9 cut(s) 534, 710, 716, 770, 888, 964, 1220, 1244, 1422
NlaIV GGNNCC 5 cut(s) 294, 306, 483, 1147, 1148
NmuCI GTSAC 3 cut(s) 925, 974, 1363
NspI RCATGY 3 cut(s) 534, 710, 888
PagI TCATGA 2 cut(s) 712, 1240
PciSI GCTCTTC 1 cut(s) 1302
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PfeI GAWTC 5 cut(s) 172, 358, 379, 545, 730
PkrI GCNGC 7 cut(s) 17, 137, 439, 784, 787, 1477, 1501
PleI GAGTC 4 cut(s) 80, 397, 683, 907
PpsI GAGTC 4 cut(s) 80, 397, 683, 907
PpuMI RGGWCCY 1 cut(s) 1146
Psp5II RGGWCCY 1 cut(s) 1146
Psp6I CCWGG 2 cut(s) 747, 1009
PspEI GGTNACC 1 cut(s) 986
PspFI CCCAGC 1 cut(s) 1135
PspGI CCWGG 2 cut(s) 747, 1009
PspN4I GGNNCC 5 cut(s) 294, 306, 483, 1147, 1148
PspPI GGNCC 2 cut(s) 740, 1146
PspPPI RGGWCCY 1 cut(s) 1146
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 4 cut(s) 304, 401, 481, 1069
PvuII CAGCTG 1 cut(s) 788
RsaI GTAC 3 cut(s) 277, 762, 898
RsaNI GTAC 3 cut(s) 276, 761, 897
RseI CAYNNNNRTG 3 cut(s) 222, 711, 883
SalI GTCGAC 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 1302
SaqAI TTAA 4 cut(s) 518, 1065, 1268, 1322
SatI GCNGC 7 cut(s) 16, 136, 438, 783, 786, 1476, 1500
Sau3AI GATC 8 cut(s) 304, 401, 481, 571, 619, 1069, 1182, 1243
Sau96I GGNCC 2 cut(s) 740, 1146
SchI GAGTC 4 cut(s) 80, 398, 683, 907
ScrFI CCNGG 4 cut(s) 12, 301, 749, 1011
SfaNI GCATC 4 cut(s) 303, 731, 974, 1462
SfcI CTRYAG 1 cut(s) 501
SgrDI CGTCGACG 1 cut(s) 47
SinI GGWCC 2 cut(s) 740, 1146
SmiMI CAYNNNNRTG 3 cut(s) 222, 711, 883
SmlI CTYRAG 1 cut(s) 1124
SmoI CTYRAG 1 cut(s) 1124
SspMI CTAG 1 cut(s) 674
StyD4I CCNGG 4 cut(s) 10, 299, 747, 1009
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 2 cut(s) 169, 415
TaiI ACGT 1 cut(s) 54
TaqI TCGA 7 cut(s) 48, 66, 114, 207, 844, 916, 1173
TatI WGTACW 1 cut(s) 896
TauI GCSGC 2 cut(s) 138, 1502
TfiI GAWTC 5 cut(s) 172, 358, 379, 545, 730
Tru1I TTAA 4 cut(s) 518, 1065, 1268, 1322
Tru9I TTAA 4 cut(s) 518, 1065, 1268, 1322
TscAI CASTG 4 cut(s) 172, 496, 675, 1492
TseFI GTSAC 3 cut(s) 925, 974, 1363
TseI GCWGC 5 cut(s) 15, 437, 782, 785, 1475
Tsp45I GTSAC 3 cut(s) 925, 974, 1363
TspDTI ATGAA 3 cut(s) 580, 1342, 1391
TspRI CASTG 4 cut(s) 172, 496, 675, 1492
VpaK11BI GGWCC 2 cut(s) 740, 1146
XagI CCTNNNNNAGG 1 cut(s) 1029
XapI RAATTY 4 cut(s) 825, 1020, 1113, 1191
XceI RCATGY 3 cut(s) 534, 710, 888
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 1 cut(s) 674
ZraI GACGTC 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.