Rw5G023960

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000885
Physical Location & Seq
Forward (+)
33964312 .. 33966102
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023960.1

Sequence Viewer

Length: 1071 bp
ATGGGTGACGTCAGACTTGGGGTTAATTATGGCGGAAAGTGGGCTGGTTCTGTTTACATAGGTGGCAGCAGGACAGAAGAAATAGTGGTTTCAGAAGACATTACTTATAATGAGCTTCTAGATCAAATGTATTATATAGTTGGAGTGGATCCAAGTGAAAATGAGATCAAGATCAGCACGATCGATGAATTAAAATCACCAGCTTATCCTGTGGAGATAATCGATGATGATGACCTAAAAACTTTTATTGATCAGAGCCGTTCGTCCAATATGCACTCAAAGCATCCATTGCAGAATGTCTCTGGGAGAATGACTGAAATTAGAGTTTTGGTTAATTATGGCGGGGAGTGGGTGAATTCTACATACGTTGGTGGCAAAACGAAAGGAATAGTAATCTCAAAGGACATTACATATCAAGAACTTGTGGCTAGAGTGTATGGTGTTGTGGGAGTTGATCCAAATGAATATAAGCTGATTATGAAGACCGCATATGAATCAAATCTACCAACTCAGCCTCTGGAGATAATCGATGATGAAGGCCTTGCATTTTTCATTCATGAAGTTTTACTGGGTATGAGCTCCATAGTTACTTTGTGCACTACCCTTGAAGGATGGGCATTTCCTAATGGAAGGATGGAGTCATCACAAAGTAGTGCGACTACACATCTTAGAAGATTTAAGACCTATCAGAGTTGCCCTCATTGTGTGACACCTGATCAGATGCCTCATTTTCAAACTCAAGTTGGATTATCGTCACCAAATGTGCCTTGTATGCAGCAGCAACAACCTTTCTGCCAGCAACAACAACATTCTTACCAACAAACAACGTCGCAGCCTTGCTACGAGCACTATCAATTCCCATCCTTTCAGCAGTCGCAGCCCCCGTACCAAGAACTTCATCCTTCGTACATGCAGCCACCGCCACCAATTCATCGTGTGCCTTATTCGCTTACAATGTTCAGGAATGATGCAACCCGAATATTTCAATATTTCAATTTTTGGCAATTTTGTTTCGTCATCATTTTATTAGGAAATATTTCCCATTTCTCAAGGCTTTGTAGAAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

356

Amino Acids

40.69

Weight (kDa)

5.44

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 108
AatII GACGTC 1 cut(s) 12
AciI CCGC 4 cut(s) 33, 342, 486, 920
AclWI GGATC 3 cut(s) 143, 156, 449
AcsI RAATTY 1 cut(s) 355
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 2 cut(s) 887, 908
AgsI TTSAA 4 cut(s) 608, 734, 986, 994
AluBI AGCT 4 cut(s) 115, 203, 472, 579
AluI AGCT 4 cut(s) 115, 203, 472, 579
Alw21I GWGCWC 3 cut(s) 581, 599, 849
Alw26I GTCTC 1 cut(s) 304
Alw44I GTGCAC 1 cut(s) 595
AlwI GGATC 3 cut(s) 143, 156, 449
AlwNI CAGNNNCTG 1 cut(s) 517
AoxI GGCC 1 cut(s) 538
ApaLI GTGCAC 1 cut(s) 595
ApeKI GCWGC 6 cut(s) 66, 775, 778, 832, 877, 913
ApoI RAATTY 1 cut(s) 355
Asp700I GAANNNNTTC 1 cut(s) 1036
AsuHPI GGTGA 4 cut(s) 17, 189, 364, 747
BaeGI GKGCMC 1 cut(s) 599
BamHI GGATCC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 581
BbsI GAAGAC 2 cut(s) 102, 488
Bbv12I GWGCWC 3 cut(s) 581, 599, 849
BbvI GCAGC 6 cut(s) 78, 787, 790, 844, 889, 925
BccI CCATC 3 cut(s) 606, 628, 868
BceAI ACGGC 1 cut(s) 243
BclI TGATCA 2 cut(s) 250, 715
BcoDI GTCTC 1 cut(s) 304
BfaI CTAG 2 cut(s) 119, 429
BisI GCNGC 6 cut(s) 67, 776, 779, 833, 878, 914
BlsI GCNGC 6 cut(s) 68, 777, 780, 834, 879, 915
BmiI GGNNCC 1 cut(s) 150
BmrI ACTGGG 1 cut(s) 578
BmsI GCATC 3 cut(s) 292, 711, 958
BmuI ACTGGG 1 cut(s) 578
BpiI GAAGAC 2 cut(s) 102, 488
BplI GAGNNNNNCTC 2 cut(s) 682, 714
BpmI CTGGAG 1 cut(s) 539
BpuEI CTTGAG 2 cut(s) 723, 1033
Bsa29I ATCGAT 3 cut(s) 183, 222, 528
BsaBI GATNNNNATC 1 cut(s) 170
BsaHI GRCGYC 1 cut(s) 9
Bse1I ACTGG 1 cut(s) 573
Bse3DI GCAATG 1 cut(s) 287
Bse8I GATNNNNATC 1 cut(s) 170
BseCI ATCGAT 3 cut(s) 183, 222, 528
BseGI GGATG 5 cut(s) 283, 617, 639, 860, 898
BseJI GATNNNNATC 1 cut(s) 170
BseMI GCAATG 1 cut(s) 287
BseMII CTCAG 1 cut(s) 524
BseNI ACTGG 1 cut(s) 573
BseSI GKGCMC 1 cut(s) 599
BseXI GCAGC 6 cut(s) 78, 787, 790, 844, 889, 925
Bsh1285I CGRYCG 1 cut(s) 183
BshFI GGCC 1 cut(s) 540
BshVI ATCGAT 3 cut(s) 183, 222, 528
BsiEI CGRYCG 1 cut(s) 183
BsiHKAI GWGCWC 3 cut(s) 581, 599, 849
BsmAI GTCTC 1 cut(s) 304
BsnI GGCC 1 cut(s) 540
Bsp1286I GDGCHC 3 cut(s) 581, 599, 849
Bsp143I GATC 8 cut(s) 121, 148, 165, 171, 180, 250, 454, 715
BspACI CCGC 4 cut(s) 33, 342, 486, 920
BspANI GGCC 1 cut(s) 540
BspCNI CTCAG 1 cut(s) 523
BspDI ATCGAT 3 cut(s) 183, 222, 528
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 1 cut(s) 150
BspPI GGATC 3 cut(s) 143, 156, 449
BsrDI GCAATG 1 cut(s) 287
BsrI ACTGG 1 cut(s) 573
BssMI GATC 8 cut(s) 121, 148, 165, 171, 180, 250, 454, 715
BssNI GRCGYC 1 cut(s) 9
BstACI GRCGYC 1 cut(s) 9
BstAPI GCANNNNNTGC 1 cut(s) 289
BstC8I GCNNGC 1 cut(s) 797
BstDEI CTNAG 2 cut(s) 510, 668
BstF5I GGATG 5 cut(s) 283, 617, 639, 860, 898
BstKTI GATC 8 cut(s) 124, 151, 168, 174, 183, 253, 457, 718
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 8 cut(s) 121, 148, 165, 171, 180, 250, 454, 715
BstMCI CGRYCG 1 cut(s) 183
BstMWI GCNNNNNNNGC 6 cut(s) 280, 289, 772, 877, 919, 946
BstNSI RCATGY 1 cut(s) 913
BstSLI GKGCMC 1 cut(s) 599
BstV1I GCAGC 6 cut(s) 78, 787, 790, 844, 889, 925
BstV2I GAAGAC 2 cut(s) 102, 488
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
Bsu15I ATCGAT 3 cut(s) 183, 222, 528
BsuRI GGCC 1 cut(s) 540
BsuTUI ATCGAT 3 cut(s) 183, 222, 528
BtsCI GGATG 5 cut(s) 283, 617, 639, 860, 898
Cac8I GCNNGC 1 cut(s) 797
CaiI CAGNNNCTG 1 cut(s) 517
CciI TCATGA 1 cut(s) 556
ClaI ATCGAT 3 cut(s) 183, 222, 528
Csp6I GTAC 2 cut(s) 886, 907
CviAII CATG 2 cut(s) 557, 910
CviQI GTAC 2 cut(s) 886, 907
DdeI CTNAG 2 cut(s) 510, 668
DpnI GATC 8 cut(s) 123, 150, 167, 173, 182, 252, 456, 717
DpnII GATC 8 cut(s) 121, 148, 165, 171, 180, 250, 454, 715
EciI GGCGGA 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 579
Eco147I AGGCCT 1 cut(s) 540
Eco24I GRGCYC 1 cut(s) 581
Eco53kI GAGCTC 1 cut(s) 579
EcoICRI GAGCTC 1 cut(s) 579
EcoRI GAATTC 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 581
FaeI CATG 2 cut(s) 560, 913
FatI CATG 2 cut(s) 556, 909
FauI CCCGC 1 cut(s) 335
FauNDI CATATG 1 cut(s) 490
FbaI TGATCA 2 cut(s) 250, 715
Fnu4HI GCNGC 6 cut(s) 67, 776, 779, 833, 878, 914
FokI GGATG 5 cut(s) 270, 624, 646, 847, 885
FriOI GRGCYC 1 cut(s) 581
Fsp4HI GCNGC 6 cut(s) 67, 776, 779, 833, 878, 914
FspBI CTAG 2 cut(s) 119, 429
GluI GCNGC 6 cut(s) 67, 776, 779, 833, 878, 914
GsuI CTGGAG 1 cut(s) 539
HaeIII GGCC 1 cut(s) 540
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 2 cut(s) 560, 913
HinfI GANTC 2 cut(s) 494, 638
HphI GGTGA 4 cut(s) 17, 189, 364, 747
Hpy166II GTNNAC 2 cut(s) 55, 597
Hpy188I TCNGA 5 cut(s) 14, 94, 255, 690, 720
Hpy188III TCNNGA 6 cut(s) 119, 169, 416, 518, 557, 961
Hpy8I GTNNAC 2 cut(s) 55, 597
Hpy99I CGWCG 1 cut(s) 832
HpyAV CCTTC 4 cut(s) 530, 602, 624, 912
HpyCH4IV ACGT 3 cut(s) 9, 366, 827
HpyCH4V TGCA 7 cut(s) 274, 292, 545, 597, 775, 913, 971
HpyF10VI GCNNNNNNNGC 6 cut(s) 280, 289, 772, 877, 919, 946
HpyF3I CTNAG 2 cut(s) 510, 668
HpySE526I ACGT 3 cut(s) 9, 366, 827
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 2 cut(s) 560, 913
Ksp22I TGATCA 2 cut(s) 250, 715
Kzo9I GATC 8 cut(s) 121, 148, 165, 171, 180, 250, 454, 715
LmnI GCTCC 1 cut(s) 584
Lsp1109I GCAGC 6 cut(s) 78, 787, 790, 844, 889, 925
LweI GCATC 3 cut(s) 292, 711, 958
MaeI CTAG 2 cut(s) 119, 429
MaeII ACGT 3 cut(s) 9, 366, 827
MaeIII GTNAC 4 cut(s) 5, 586, 706, 753
MalI GATC 8 cut(s) 123, 150, 167, 173, 182, 252, 456, 717
MboI GATC 8 cut(s) 121, 148, 165, 171, 180, 250, 454, 715
MboII GAAGA 4 cut(s) 89, 107, 493, 684
MflI RGATCY 1 cut(s) 148
MhlI GDGCHC 3 cut(s) 581, 599, 849
MlyI GAGTC 1 cut(s) 647
MmeI TCCRAC 2 cut(s) 121, 724
MnlI CCTC 3 cut(s) 525, 708, 735
MroXI GAANNNNTTC 1 cut(s) 1036
MseI TTAA 4 cut(s) 24, 191, 333, 678
MwoI GCNNNNNNNGC 6 cut(s) 280, 289, 772, 877, 919, 946
NdeI CATATG 1 cut(s) 490
NdeII GATC 8 cut(s) 121, 148, 165, 171, 180, 250, 454, 715
NlaIII CATG 2 cut(s) 560, 913
NlaIV GGNNCC 1 cut(s) 150
NmuCI GTSAC 3 cut(s) 5, 706, 753
NspI RCATGY 1 cut(s) 913
PagI TCATGA 1 cut(s) 556
PceI AGGCCT 1 cut(s) 540
PcsI WCGNNNNNNNCGW 1 cut(s) 881
PdmI GAANNNNTTC 1 cut(s) 1036
PfeI GAWTC 1 cut(s) 494
PkrI GCNGC 6 cut(s) 68, 777, 780, 834, 879, 915
Ple19I CGATCG 1 cut(s) 183
PleI GAGTC 1 cut(s) 646
PpsI GAGTC 1 cut(s) 646
PsiI TTATAA 1 cut(s) 108
Psp124BI GAGCTC 1 cut(s) 581
PspN4I GGNNCC 1 cut(s) 150
PstNI CAGNNNCTG 1 cut(s) 517
PsuI RGATCY 1 cut(s) 148
PvuI CGATCG 1 cut(s) 183
RsaI GTAC 2 cut(s) 887, 908
RsaNI GTAC 2 cut(s) 886, 907
SacI GAGCTC 1 cut(s) 581
SaqAI TTAA 4 cut(s) 24, 191, 333, 678
SatI GCNGC 6 cut(s) 67, 776, 779, 833, 878, 914
Sau3AI GATC 8 cut(s) 121, 148, 165, 171, 180, 250, 454, 715
SchI GAGTC 1 cut(s) 647
SduI GDGCHC 3 cut(s) 581, 599, 849
SfaNI GCATC 3 cut(s) 292, 711, 958
SmlI CTYRAG 2 cut(s) 738, 1048
SmoI CTYRAG 2 cut(s) 738, 1048
SseBI AGGCCT 1 cut(s) 540
SsiI CCGC 4 cut(s) 33, 342, 486, 920
SspI AATATT 3 cut(s) 981, 989, 1036
SspMI CTAG 2 cut(s) 119, 429
SstI GAGCTC 1 cut(s) 581
StuI AGGCCT 1 cut(s) 540
TaiI ACGT 3 cut(s) 12, 369, 830
TaqI TCGA 3 cut(s) 183, 222, 528
TfiI GAWTC 1 cut(s) 494
Tru1I TTAA 4 cut(s) 24, 191, 333, 678
Tru9I TTAA 4 cut(s) 24, 191, 333, 678
TseFI GTSAC 3 cut(s) 5, 706, 753
TseI GCWGC 6 cut(s) 66, 775, 778, 832, 877, 913
Tsp45I GTSAC 3 cut(s) 5, 706, 753
VneI GTGCAC 1 cut(s) 595
XapI RAATTY 1 cut(s) 355
XbaI TCTAGA 1 cut(s) 118
XceI RCATGY 1 cut(s) 913
XmnI GAANNNNTTC 1 cut(s) 1036
XspI CTAG 2 cut(s) 119, 429
ZraI GACGTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.