RchiOBHm_Chr5g0035231

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000885
Physical Location & Seq
Forward (+)
29139552 .. 29149172
9621 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31414

Sequence Viewer

Length: 1485 bp
ATGCCAAAGCATCTTCACAGCGAGCTTCAGCACGACACAAAACCACCATACCCTTGTTCCTTTTGGGGAGCAGTATCTACTGTAAATTTGCGCGAAGAAGAACTCATTCAAGATGTTGTTAAAGTGATATGGAGCAAATTGCATCCCTGTACTTCAAAATCAGCCATAAACAATGTGTCATCAATACCGTCTTCGTCCTCTTCTTTTTGTCGACCAAAGCATGATGTCTTTCTAAGCTTTAGAGGGGCGGATACCCGCGCATTTATGGGCCATCTACATGCTGCATTAATTCACCGCGGAATTTCCACCTTTAGAGATGACAAAGATCTTGAGAAAGGAAAGTCCATTCCCAAAGAACTCGACAAAGCAATTGAGGGATCAAAGGTTTGTGTTGTCATCGTTTCACCAGACTATGCTTCTTCAAAATGGTGCTTGAATGAGCTTTTGAAGATTCTTGAATGCGCTTTTGAAGATTCTTCCTTACCGCAACATCATGAAAAAGCCCATAATTTGGAGAGCAGATACAAAATAAGTAAGTGGAAGGCTAGTTTGCAGAACAAGGAAGACATCTCTAATCATAACAAAAGGAAAATGGTTAAGCTTAGCCTCACGGTTAATTATGATGGAGAATGGGCCAGTCCTGTTTACACTGGTGATAAAACAAAAGAAATAGTGATTTCAGATGACAGTACTCCTGATGAGCTTCTGGATCAAATACATAGTTTTGTTGGGGTGGATCCAAAACAAAATGAGAGCAATACTGATGATAACCAAAGAATTTTTATTGGTAAGAGCCTTTCTTCCAGTACGGGCTTCAAGAATCCACTGCGGAATGCTTGTGAAAGAAGAATGAATGAAGTTCGAGTTTTGGTGAATTATGACGGGGAGTGGGTGAATTCTACATACGTTGGTGGTCAAACGAAAGGAATAGTGATTTCAGAGGATATTGCGTATCAAGGACTAGTGGAGAGAGTGTATGGTGTTGTCGGAGTTGATCCACATGAATATAAGCTGATATTGAAGACCAGATATGAATCAAAGTTTCCTACTCAACCGGTGGAGATAATCAATGATGATGACCTTGCATTCTTCATCAAAGAGAACCTTTCACGGGATATAAGCTCCCGAATTCCTTTGTGCACGACTCTTGAACGACGGTCATTTCCTTATGGAAGGGTGGAGTCATCAGAGAACAGCAATACGGCACATATTAACAGATTTGGGACCTATCACAGTTACTTTCATGGTTGGACACCTGATCCAGTGCCTTATCTGCAGCAGCCTTTCTACCAGCAGCAACAACCGTCTTACCAACAAACGTGGACATGGCAGCCTTACTACAAACCTTCCTATCAGCAGCTGCAATGGGGATACCAACAGCCCCATCATTCCTACATGCCGCCACAATCTTCATGCCAACAGCCCCCCGAAATCTTCTGGCATCACTATCAGCCCCATACGTACCCACCAACATCTTACCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

494

Amino Acids

56.94

Weight (kDa)

6.62

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 73 - 158 8e-27 TIR domain
TIR_2 PF13676 76 - 155 2.3e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 511
AccI GTMKAC 1 cut(s) 211
AccII CGCG 3 cut(s) 93, 258, 297
AciI CCGC 7 cut(s) 248, 256, 295, 297, 485, 829, 1400
AclWI GGATC 6 cut(s) 385, 717, 731, 744, 989, 1253
AcsI RAATTY 5 cut(s) 85, 300, 777, 895, 1128
AcuI CTGAAG 1 cut(s) 11
AfaI GTAC 4 cut(s) 151, 691, 808, 1463
AfiI CCNNNNNNNGG 2 cut(s) 511, 1111
AgeI ACCGGT 1 cut(s) 1054
AhlI ACTAGT 1 cut(s) 961
AluBI AGCT 8 cut(s) 25, 237, 442, 601, 703, 1012, 1122, 1360
AluI AGCT 8 cut(s) 25, 237, 442, 601, 703, 1012, 1122, 1360
Alw21I GWGCWC 1 cut(s) 1142
Alw44I GTGCAC 1 cut(s) 1138
AlwI GGATC 6 cut(s) 385, 717, 731, 744, 989, 1253
AlwNI CAGNNNCTG 1 cut(s) 1360
AoxI GGCC 2 cut(s) 268, 633
ApaLI GTGCAC 1 cut(s) 1138
ApeKI GCWGC 7 cut(s) 281, 1276, 1279, 1294, 1330, 1357, 1360
ApoI RAATTY 5 cut(s) 85, 300, 777, 895, 1128
AseI ATTAAT 1 cut(s) 287
AsiGI ACCGGT 1 cut(s) 1054
Asp700I GAANNNNTTC 1 cut(s) 105
AspLEI GCGC 3 cut(s) 93, 260, 464
AspS9I GGNCC 3 cut(s) 268, 633, 1224
AsuHPI GGTGA 5 cut(s) 284, 396, 665, 883, 904
AvaII GGWCC 1 cut(s) 1224
BaeGI GKGCMC 1 cut(s) 1142
BamHI GGATCC 1 cut(s) 736
BbsI GAAGAC 3 cut(s) 183, 570, 1028
Bbv12I GWGCWC 1 cut(s) 1142
BbvI GCAGC 7 cut(s) 268, 1288, 1291, 1306, 1342, 1347, 1369
BccI CCATC 3 cut(s) 279, 617, 1392
BceAI ACGGC 1 cut(s) 1218
BciVI GTATCC 2 cut(s) 244, 1364
BcuI ACTAGT 1 cut(s) 961
BfaI CTAG 2 cut(s) 546, 962
BfmI CTRYAG 1 cut(s) 1274
BfuI GTATCC 2 cut(s) 244, 1364
BglII AGATCT 1 cut(s) 325
BisI GCNGC 8 cut(s) 282, 1277, 1280, 1295, 1331, 1358, 1361, 1400
BlpI GCTNAGC 1 cut(s) 602
BlsI GCNGC 8 cut(s) 283, 1278, 1281, 1296, 1332, 1359, 1362, 1401
BmcAI AGTACT 1 cut(s) 691
Bme18I GGWCC 1 cut(s) 1224
BmgT120I GGNCC 3 cut(s) 268, 633, 1224
BmiI GGNNCC 2 cut(s) 738, 1225
BmsI GCATC 3 cut(s) 19, 151, 1450
BpiI GAAGAC 3 cut(s) 183, 570, 1028
Bpu1102I GCTNAGC 1 cut(s) 602
BpuEI CTTGAG 1 cut(s) 350
BsaAI YACGTR 1 cut(s) 1461
BsaBI GATNNNNATC 1 cut(s) 1033
BsaJI CCNNGG 1 cut(s) 295
BsaWI WCCGGW 1 cut(s) 1054
BsaXI ACNNNNNCTCC 2 cut(s) 959, 989
Bsc4I CCNNNNNNNGG 2 cut(s) 511, 1111
Bse118I RCCGGY 1 cut(s) 1054
Bse1I ACTGG 5 cut(s) 636, 655, 804, 1262, 1480
Bse3DI GCAATG 1 cut(s) 1370
Bse8I GATNNNNATC 1 cut(s) 1033
BseDI CCNNGG 1 cut(s) 295
BseGI GGATG 1 cut(s) 142
BseJI GATNNNNATC 1 cut(s) 1033
BseLI CCNNNNNNNGG 2 cut(s) 511, 1111
BseMI GCAATG 1 cut(s) 1370
BseNI ACTGG 5 cut(s) 636, 655, 804, 1262, 1480
BseSI GKGCMC 1 cut(s) 1142
BseXI GCAGC 7 cut(s) 268, 1288, 1291, 1306, 1342, 1347, 1369
Bsh1236I CGCG 3 cut(s) 93, 258, 297
BshFI GGCC 2 cut(s) 270, 635
BshTI ACCGGT 1 cut(s) 1054
BsiHKAI GWGCWC 1 cut(s) 1142
BsiSI CCGG 1 cut(s) 1055
BslFI GGGAC 1 cut(s) 1237
BslI CCNNNNNNNGG 2 cut(s) 511, 1111
BsmFI GGGAC 1 cut(s) 1237
BsmI GAATGC 3 cut(s) 464, 838, 1085
BsnI GGCC 2 cut(s) 270, 635
Bsp1286I GDGCHC 1 cut(s) 1142
Bsp143I GATC 6 cut(s) 325, 377, 709, 736, 994, 1258
Bsp1720I GCTNAGC 1 cut(s) 602
BspACI CCGC 7 cut(s) 248, 256, 295, 297, 485, 829, 1400
BspANI GGCC 2 cut(s) 270, 635
BspFNI CGCG 3 cut(s) 93, 258, 297
BspHI TCATGA 1 cut(s) 493
BspLI GGNNCC 2 cut(s) 738, 1225
BspMAI CTGCAG 1 cut(s) 1278
BspPI GGATC 6 cut(s) 385, 717, 731, 744, 989, 1253
BsrDI GCAATG 1 cut(s) 1370
BsrFI RCCGGY 1 cut(s) 1054
BsrI ACTGG 5 cut(s) 636, 655, 804, 1262, 1480
BssAI RCCGGY 1 cut(s) 1054
BssECI CCNNGG 1 cut(s) 295
BssMI GATC 6 cut(s) 325, 377, 709, 736, 994, 1258
Bst4CI ACNGT 7 cut(s) 82, 189, 613, 689, 1158, 1235, 1305
Bst6I CTCTTC 1 cut(s) 205
BstBAI YACGTR 1 cut(s) 1461
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 2 cut(s) 233, 602
BstDSI CCRYGG 1 cut(s) 295
BstF5I GGATG 1 cut(s) 142
BstFNI CGCG 3 cut(s) 93, 258, 297
BstHHI GCGC 3 cut(s) 93, 260, 464
BstKTI GATC 6 cut(s) 328, 380, 712, 739, 997, 1261
BstMBI GATC 6 cut(s) 325, 377, 709, 736, 994, 1258
BstMWI GCNNNNNNNGC 1 cut(s) 1273
BstNSI RCATGY 2 cut(s) 281, 1399
BstSFI CTRYAG 1 cut(s) 1274
BstSLI GKGCMC 1 cut(s) 1142
BstSNI TACGTA 1 cut(s) 1461
BstUI CGCG 3 cut(s) 93, 258, 297
BstV1I GCAGC 7 cut(s) 268, 1288, 1291, 1306, 1342, 1347, 1369
BstV2I GAAGAC 3 cut(s) 183, 570, 1028
BstX2I RGATCY 2 cut(s) 325, 736
BstYI RGATCY 2 cut(s) 325, 736
BsuI GTATCC 2 cut(s) 244, 1364
BsuRI GGCC 2 cut(s) 270, 635
BtgI CCRYGG 1 cut(s) 295
BtsCI GGATG 1 cut(s) 142
BtsI GCAGTG 1 cut(s) 824
BtsIMutI CAGTG 3 cut(s) 648, 824, 1269
Cac8I GCNNGC 1 cut(s) 23
CaiI CAGNNNCTG 1 cut(s) 1360
CciI TCATGA 1 cut(s) 493
CfoI GCGC 3 cut(s) 93, 260, 464
Cfr10I RCCGGY 1 cut(s) 1054
Cfr13I GGNCC 3 cut(s) 268, 633, 1224
Cfr42I CCGCGG 1 cut(s) 298
Csp6I GTAC 4 cut(s) 150, 690, 807, 1462
CspAI ACCGGT 1 cut(s) 1054
CspCI CAANNNNNGTGG 2 cut(s) 1301, 1336
CviAII CATG 8 cut(s) 221, 278, 494, 1001, 1244, 1326, 1396, 1413
CviQI GTAC 4 cut(s) 150, 690, 807, 1462
DdeI CTNAG 2 cut(s) 233, 602
DpnI GATC 6 cut(s) 327, 379, 711, 738, 996, 1260
DpnII GATC 6 cut(s) 325, 377, 709, 736, 994, 1258
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
EciI GGCGGA 1 cut(s) 263
Eco105I TACGTA 1 cut(s) 1461
Eco47I GGWCC 1 cut(s) 1224
Eco57I CTGAAG 1 cut(s) 11
EcoO109I RGGNCCY 1 cut(s) 1224
EcoRI GAATTC 2 cut(s) 895, 1128
FaeI CATG 8 cut(s) 224, 281, 497, 1004, 1247, 1329, 1399, 1416
FalI AAGNNNNNCTT 2 cut(s) 1089, 1121
FaqI GGGAC 1 cut(s) 1237
FatI CATG 8 cut(s) 220, 277, 493, 1000, 1243, 1325, 1395, 1412
FauI CCCGC 1 cut(s) 263
FblI GTMKAC 1 cut(s) 211
Fnu4HI GCNGC 8 cut(s) 282, 1277, 1280, 1295, 1331, 1358, 1361, 1400
FokI GGATG 1 cut(s) 129
Fsp4HI GCNGC 8 cut(s) 282, 1277, 1280, 1295, 1331, 1358, 1361, 1400
FspBI CTAG 2 cut(s) 546, 962
GlaI GCGC 3 cut(s) 92, 259, 463
GluI GCNGC 8 cut(s) 282, 1277, 1280, 1295, 1331, 1358, 1361, 1400
HaeIII GGCC 2 cut(s) 270, 635
HapII CCGG 1 cut(s) 1055
HhaI GCGC 3 cut(s) 93, 260, 464
Hin1II CATG 8 cut(s) 224, 281, 497, 1004, 1247, 1329, 1399, 1416
Hin6I GCGC 3 cut(s) 91, 258, 462
HinP1I GCGC 3 cut(s) 91, 258, 462
HincII GTYRAC 1 cut(s) 212
HindII GTYRAC 1 cut(s) 212
HindIII AAGCTT 2 cut(s) 235, 599
HinfI GANTC 6 cut(s) 451, 473, 820, 1034, 1144, 1181
HpaII CCGG 1 cut(s) 1055
HphI GGTGA 5 cut(s) 284, 396, 665, 883, 904
Hpy166II GTNNAC 4 cut(s) 212, 646, 1140, 1323
Hpy188I TCNGA 4 cut(s) 682, 940, 989, 1189
Hpy188III TCNNGA 9 cut(s) 110, 329, 455, 494, 695, 707, 817, 1125, 1148
Hpy8I GTNNAC 4 cut(s) 212, 646, 1140, 1323
Hpy99I CGWCG 1 cut(s) 1158
HpyAV CCTTC 3 cut(s) 535, 1167, 1356
HpyCH4III ACNGT 7 cut(s) 82, 189, 613, 689, 1158, 1235, 1305
HpyCH4IV ACGT 3 cut(s) 906, 1319, 1460
HpyCH4V TGCA 7 cut(s) 142, 284, 553, 1085, 1140, 1276, 1363
HpyF10VI GCNNNNNNNGC 1 cut(s) 1273
HpyF3I CTNAG 2 cut(s) 233, 602
HpySE526I ACGT 3 cut(s) 906, 1319, 1460
Hsp92II CATG 8 cut(s) 224, 281, 497, 1004, 1247, 1329, 1399, 1416
HspAI GCGC 3 cut(s) 91, 258, 462
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 6 cut(s) 325, 377, 709, 736, 994, 1258
LmnI GCTCC 3 cut(s) 68, 132, 1127
Lsp1109I GCAGC 7 cut(s) 268, 1288, 1291, 1306, 1342, 1347, 1369
LweI GCATC 3 cut(s) 19, 151, 1450
MaeI CTAG 2 cut(s) 546, 962
MaeII ACGT 3 cut(s) 906, 1319, 1460
MaeIII GTNAC 1 cut(s) 1235
MalI GATC 6 cut(s) 327, 379, 711, 738, 996, 1260
MboI GATC 6 cut(s) 325, 377, 709, 736, 994, 1258
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 2 cut(s) 325, 736
MhlI GDGCHC 1 cut(s) 1142
MlyI GAGTC 2 cut(s) 1138, 1190
MmeI TCCRAC 2 cut(s) 967, 1229
MnlI CCTC 5 cut(s) 208, 236, 367, 617, 934
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 5 cut(s) 120, 287, 597, 615, 1212
MslI CAYNNNNRTG 1 cut(s) 276
MspA1I CMGCKG 2 cut(s) 297, 1360
MspI CCGG 1 cut(s) 1055
MunI CAATTG 1 cut(s) 369
Mva1269I GAATGC 3 cut(s) 464, 838, 1085
MvnI CGCG 3 cut(s) 93, 258, 297
MwoI GCNNNNNNNGC 1 cut(s) 1273
NdeII GATC 6 cut(s) 325, 377, 709, 736, 994, 1258
NlaIII CATG 8 cut(s) 224, 281, 497, 1004, 1247, 1329, 1399, 1416
NlaIV GGNNCC 2 cut(s) 738, 1225
NspI RCATGY 2 cut(s) 281, 1399
PagI TCATGA 1 cut(s) 493
PctI GAATGC 3 cut(s) 464, 838, 1085
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 4 cut(s) 451, 473, 820, 1034
PflMI CCANNNNNTGG 1 cut(s) 511
PinAI ACCGGT 1 cut(s) 1054
PkrI GCNGC 8 cut(s) 283, 1278, 1281, 1296, 1332, 1359, 1362, 1401
PleI GAGTC 2 cut(s) 1138, 1189
PpsI GAGTC 2 cut(s) 1138, 1189
Ppu21I YACGTR 1 cut(s) 1461
PpuMI RGGWCCY 1 cut(s) 1224
PshBI ATTAAT 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 1224
PspN4I GGNNCC 2 cut(s) 738, 1225
PspPI GGNCC 3 cut(s) 268, 633, 1224
PspPPI RGGWCCY 1 cut(s) 1224
PstI CTGCAG 1 cut(s) 1278
PstNI CAGNNNCTG 1 cut(s) 1360
PsuI RGATCY 2 cut(s) 325, 736
PvuII CAGCTG 1 cut(s) 1360
RsaI GTAC 4 cut(s) 151, 691, 808, 1463
RsaNI GTAC 4 cut(s) 150, 690, 807, 1462
RseI CAYNNNNRTG 1 cut(s) 276
SacII CCGCGG 1 cut(s) 298
SalI GTCGAC 1 cut(s) 210
SaqAI TTAA 5 cut(s) 120, 287, 597, 615, 1212
SatI GCNGC 8 cut(s) 282, 1277, 1280, 1295, 1331, 1358, 1361, 1400
Sau3AI GATC 6 cut(s) 325, 377, 709, 736, 994, 1258
Sau96I GGNCC 3 cut(s) 268, 633, 1224
ScaI AGTACT 1 cut(s) 691
SchI GAGTC 2 cut(s) 1138, 1190
SduI GDGCHC 1 cut(s) 1142
SfaNI GCATC 3 cut(s) 19, 151, 1450
SfcI CTRYAG 1 cut(s) 1274
Sfr303I CCGCGG 1 cut(s) 298
SgrBI CCGCGG 1 cut(s) 298
SinI GGWCC 1 cut(s) 1224
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SnaBI TACGTA 1 cut(s) 1461
SpeI ACTAGT 1 cut(s) 961
SsiI CCGC 7 cut(s) 248, 256, 295, 297, 485, 829, 1400
SspMI CTAG 2 cut(s) 546, 962
TaaI ACNGT 7 cut(s) 82, 189, 613, 689, 1158, 1235, 1305
TaiI ACGT 3 cut(s) 909, 1322, 1463
TaqI TCGA 3 cut(s) 211, 360, 862
TatI WGTACW 2 cut(s) 149, 689
TauI GCSGC 1 cut(s) 1402
TfiI GAWTC 4 cut(s) 451, 473, 820, 1034
Tru1I TTAA 5 cut(s) 120, 287, 597, 615, 1212
Tru9I TTAA 5 cut(s) 120, 287, 597, 615, 1212
TscAI CASTG 3 cut(s) 655, 831, 1269
TseI GCWGC 7 cut(s) 281, 1276, 1279, 1294, 1330, 1357, 1360
TspDTI ATGAA 8 cut(s) 510, 866, 870, 1017, 1047, 1081, 1232, 1401
TspRI CASTG 3 cut(s) 655, 831, 1269
Van91I CCANNNNNTGG 1 cut(s) 511
VneI GTGCAC 1 cut(s) 1138
VpaK11BI GGWCC 1 cut(s) 1224
VspI ATTAAT 1 cut(s) 287
XapI RAATTY 5 cut(s) 85, 300, 777, 895, 1128
XceI RCATGY 2 cut(s) 281, 1399
XmiI GTMKAC 1 cut(s) 211
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 2 cut(s) 546, 962
ZrmI AGTACT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.