Rw5G022260

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000885
Physical Location & Seq
Forward (+)
30551323 .. 30556890
5568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022260.1

Sequence Viewer

Length: 1425 bp
ATGGCTTTAATATCTCAGGACCCGTTTTGGTTACGAACGAAGTTAGATGAACATTCAAACTTTTCAGAAGCAGCAAACAATAGTGTAGAAAAAATAGTGCTGCCAATTTTCTGTGGAATCAATCCCTCTAATGTACGACATCAAACGGGAAGTTTTGCAGATCCGTTTGCAAAACATGAAGAAAAGTACAAGGATAAACCTGGAAAAGTAGAAAGGTGGAGAAGTGCTTTAAGGAAATTAGCAGATTTTGCTGGGTGGCATTCAAAGGACTGGCGCGAAGAAGAACTCATTCAAGATGTTGTTGAAGTGATATGGAGCAAATTGCATCCCTCTACTTCAAAATCAGCCATAAACAATGTGTCATCAATACCGTCTTCGTCCTCTTCTTTTTGTCGACCAAAGCATGATGTCTTTCTAAGCTTTAGAGGGGCGGATACCCGCGCATTTATGGGCCATCTACATGCTGCATTAATTCACCGCGGAATTTCCACCTTTAGAGATGACAAAGATCTTGAGAAAGGAAAGTCCATTCCCAAAGAACTCGACAAAGCAATTGAGGGATCAAAGGTTTGTGTTGTCATCGTTTCACCAGACTATGCTTCTTCAAAATGGTGCTTGAATGAGCTTTTGAAGATTCTTGAATGCGCTTTTGAAGATTCTTCCTTACCGCAACTTCATGAAAAAGCCCATAATTTGGAGAGCGGATACAAAATAAATAAGTGGAAGGCTAGTTTGCAGAACATGGAAGACATCTCTAATCATAACAAAAGGAAAATGGTTAAGCTTAGCCTCACGGTTAATTATGATGGAGAATGGGCCAGTCCTGTTTACACTGGCGATAAAACAAAAGAAATAGTGATTTCAGATGACAGTACTCCTGATGAGCTTCTGGATCAAATACATAGTTTTGTTGGGGTGGATCCAAAACAAAATGAGAGCAATGCTGATGATAACCAAAGACTTTTTATTGGTAAGAGCCTTTCTTCCAGTACGGGCTTCAAGAATCCACTGCGGAATGCTTGTGAAAGAAGAATGAGTGAAGTTCGAGTTTTGGTGAATTATGACGGGGAGTGGGTGAATTCTACATACGTTGGTGGTCAAACGAAAGGAATAGTGATTTCAGAGGATATTGCGTATCAAGGACTTGTGGAGAGAGTGTATGGTGTTGTCGGAGTTGATCCACATGTATATAAGCTGATATTGAAGACCAGATATGAATCAAAGTTTCCTACTCAACCCGTGGAGATAATCAATGATGATGACCTTGCATTCTTCATCAAAGAGAACCTTTCGCGAGATATAAGCTCCCGAATTCCTTTGTGCACGACTCTTGAACGACGGTCATTTCCTTATGGAAGGTACTGTCTTGACCTTATATTATTGTTTTTCTCTCTCGAGTCTTATGAAATCTGTACGTGCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

474

Amino Acids

53.87

Weight (kDa)

6.19

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 15 - 116 2.9e-16 TIR domain
TIR PF01582 134 - 219 7.5e-27 TIR domain
TIR_2 PF13676 137 - 216 1.9e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 694
AccBSI CCGCTC 1 cut(s) 702
AccI GTMKAC 1 cut(s) 394
AccII CGCG 4 cut(s) 276, 441, 480, 1294
AciI CCGC 7 cut(s) 431, 439, 478, 480, 668, 702, 1012
AclWI GGATC 6 cut(s) 155, 568, 900, 914, 927, 1172
AcsI RAATTY 3 cut(s) 483, 1078, 1311
AfaI GTAC 6 cut(s) 135, 188, 874, 991, 1361, 1414
AfiI CCNNNNNNNGG 1 cut(s) 694
AflIII ACRYGT 1 cut(s) 1183
AjnI CCWGG 1 cut(s) 199
AluBI AGCT 6 cut(s) 420, 625, 784, 886, 1195, 1305
AluI AGCT 6 cut(s) 420, 625, 784, 886, 1195, 1305
Alw21I GWGCWC 1 cut(s) 1325
Alw44I GTGCAC 1 cut(s) 1321
AlwI GGATC 6 cut(s) 155, 568, 900, 914, 927, 1172
Ama87I CYCGRG 1 cut(s) 1394
AoxI GGCC 2 cut(s) 451, 816
ApaLI GTGCAC 1 cut(s) 1321
ApeKI GCWGC 3 cut(s) 71, 100, 464
ApoI RAATTY 3 cut(s) 483, 1078, 1311
AseI ATTAAT 1 cut(s) 470
Asp700I GAANNNNTTC 1 cut(s) 288
AspLEI GCGC 3 cut(s) 276, 443, 647
AspS9I GGNCC 3 cut(s) 19, 451, 816
AsuHPI GGTGA 4 cut(s) 467, 579, 1066, 1087
AvaI CYCGRG 1 cut(s) 1394
AvaII GGWCC 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 1325
BamHI GGATCC 1 cut(s) 919
BbsI GAAGAC 3 cut(s) 366, 753, 1211
Bbv12I GWGCWC 1 cut(s) 1325
BbvI GCAGC 3 cut(s) 83, 87, 451
BccI CCATC 2 cut(s) 462, 800
BciT130I CCWGG 1 cut(s) 201
BciVI GTATCC 2 cut(s) 427, 698
BfaI CTAG 1 cut(s) 729
BfuI GTATCC 2 cut(s) 427, 698
BglII AGATCT 1 cut(s) 508
BisI GCNGC 3 cut(s) 72, 101, 465
BlpI GCTNAGC 1 cut(s) 785
BlsI GCNGC 3 cut(s) 73, 102, 466
BmcAI AGTACT 1 cut(s) 874
Bme1390I CCNGG 1 cut(s) 201
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 1394
BmgT120I GGNCC 3 cut(s) 19, 451, 816
BmiI GGNNCC 2 cut(s) 21, 921
BmrFI CCNGG 1 cut(s) 201
BmsI GCATC 1 cut(s) 334
BpiI GAAGAC 3 cut(s) 366, 753, 1211
Bpu1102I GCTNAGC 1 cut(s) 785
BpuEI CTTGAG 1 cut(s) 533
BsaAI YACGTR 1 cut(s) 1416
BsaBI GATNNNNATC 1 cut(s) 1216
BsaJI CCNNGG 2 cut(s) 478, 1239
BsaXI ACNNNNNCTCC 2 cut(s) 1142, 1172
Bsc4I CCNNNNNNNGG 1 cut(s) 694
Bse1I ACTGG 4 cut(s) 275, 819, 838, 987
Bse3DI GCAATG 1 cut(s) 946
Bse8I GATNNNNATC 1 cut(s) 1216
BseBI CCWGG 1 cut(s) 201
BseDI CCNNGG 2 cut(s) 478, 1239
BseGI GGATG 1 cut(s) 325
BseJI GATNNNNATC 1 cut(s) 1216
BseLI CCNNNNNNNGG 1 cut(s) 694
BseMI GCAATG 1 cut(s) 946
BseMII CTCAG 1 cut(s) 29
BseNI ACTGG 4 cut(s) 275, 819, 838, 987
BseSI GKGCMC 1 cut(s) 1325
BseXI GCAGC 3 cut(s) 83, 87, 451
BseYI CCCAGC 1 cut(s) 251
Bsh1236I CGCG 4 cut(s) 276, 441, 480, 1294
BshFI GGCC 2 cut(s) 453, 818
BsiHKAI GWGCWC 1 cut(s) 1325
BsiHKCI CYCGRG 1 cut(s) 1394
BslI CCNNNNNNNGG 1 cut(s) 694
BsmI GAATGC 4 cut(s) 259, 647, 1021, 1268
BsnI GGCC 2 cut(s) 453, 818
BsoBI CYCGRG 1 cut(s) 1394
Bsp1286I GDGCHC 1 cut(s) 1325
Bsp143I GATC 6 cut(s) 160, 508, 560, 892, 919, 1177
Bsp1720I GCTNAGC 1 cut(s) 785
Bsp68I TCGCGA 1 cut(s) 1294
BspACI CCGC 7 cut(s) 431, 439, 478, 480, 668, 702, 1012
BspANI GGCC 2 cut(s) 453, 818
BspCNI CTCAG 1 cut(s) 28
BspFNI CGCG 4 cut(s) 276, 441, 480, 1294
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 2 cut(s) 21, 921
BspPI GGATC 6 cut(s) 155, 568, 900, 914, 927, 1172
BsrBI CCGCTC 1 cut(s) 702
BsrDI GCAATG 1 cut(s) 946
BsrI ACTGG 4 cut(s) 275, 819, 838, 987
BssECI CCNNGG 2 cut(s) 478, 1239
BssMI GATC 6 cut(s) 160, 508, 560, 892, 919, 1177
Bst2UI CCWGG 1 cut(s) 201
Bst4CI ACNGT 5 cut(s) 372, 796, 872, 1341, 1364
Bst6I CTCTTC 1 cut(s) 388
BstAPI GCANNNNNTGC 1 cut(s) 248
BstBAI YACGTR 1 cut(s) 1416
BstDEI CTNAG 3 cut(s) 15, 416, 785
BstDSI CCRYGG 2 cut(s) 478, 1239
BstF5I GGATG 1 cut(s) 325
BstFNI CGCG 4 cut(s) 276, 441, 480, 1294
BstHHI GCGC 3 cut(s) 276, 443, 647
BstKTI GATC 6 cut(s) 163, 511, 563, 895, 922, 1180
BstMBI GATC 6 cut(s) 160, 508, 560, 892, 919, 1177
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstNI CCWGG 1 cut(s) 201
BstNSI RCATGY 2 cut(s) 464, 1187
BstSCI CCNGG 1 cut(s) 199
BstSLI GKGCMC 1 cut(s) 1325
BstUI CGCG 4 cut(s) 276, 441, 480, 1294
BstV1I GCAGC 3 cut(s) 83, 87, 451
BstV2I GAAGAC 3 cut(s) 366, 753, 1211
BstX2I RGATCY 3 cut(s) 160, 508, 919
BstYI RGATCY 3 cut(s) 160, 508, 919
BsuI GTATCC 2 cut(s) 427, 698
BsuRI GGCC 2 cut(s) 453, 818
BtgI CCRYGG 2 cut(s) 478, 1239
BtsCI GGATG 1 cut(s) 325
BtsI GCAGTG 1 cut(s) 1007
BtsIMutI CAGTG 2 cut(s) 831, 1007
BtuMI TCGCGA 1 cut(s) 1294
CciI TCATGA 1 cut(s) 676
CfoI GCGC 3 cut(s) 276, 443, 647
Cfr13I GGNCC 3 cut(s) 19, 451, 816
Cfr42I CCGCGG 1 cut(s) 481
Csp6I GTAC 6 cut(s) 134, 187, 873, 990, 1360, 1413
CviAII CATG 6 cut(s) 176, 404, 461, 677, 742, 1184
CviQI GTAC 6 cut(s) 134, 187, 873, 990, 1360, 1413
DdeI CTNAG 3 cut(s) 15, 416, 785
DpnI GATC 6 cut(s) 162, 510, 562, 894, 921, 1179
DpnII GATC 6 cut(s) 160, 508, 560, 892, 919, 1177
Eam1104I CTCTTC 1 cut(s) 388
EarI CTCTTC 1 cut(s) 388
EciI GGCGGA 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 19
Eco88I CYCGRG 1 cut(s) 1394
EcoO109I RGGNCCY 1 cut(s) 19
EcoRI GAATTC 2 cut(s) 1078, 1311
EcoRII CCWGG 1 cut(s) 199
FaeI CATG 6 cut(s) 179, 407, 464, 680, 745, 1187
FalI AAGNNNNNCTT 2 cut(s) 1272, 1304
FatI CATG 6 cut(s) 175, 403, 460, 676, 741, 1183
FauI CCCGC 1 cut(s) 446
FblI GTMKAC 1 cut(s) 394
Fnu4HI GCNGC 3 cut(s) 72, 101, 465
FokI GGATG 1 cut(s) 312
Fsp4HI GCNGC 3 cut(s) 72, 101, 465
FspBI CTAG 1 cut(s) 729
GlaI GCGC 3 cut(s) 275, 442, 646
GluI GCNGC 3 cut(s) 72, 101, 465
GsaI CCCAGC 1 cut(s) 255
HaeIII GGCC 2 cut(s) 453, 818
HhaI GCGC 3 cut(s) 276, 443, 647
Hin1II CATG 6 cut(s) 179, 407, 464, 680, 745, 1187
Hin6I GCGC 3 cut(s) 274, 441, 645
HinP1I GCGC 3 cut(s) 274, 441, 645
HincII GTYRAC 1 cut(s) 395
HindII GTYRAC 1 cut(s) 395
HindIII AAGCTT 2 cut(s) 418, 782
HinfI GANTC 7 cut(s) 117, 634, 656, 1003, 1217, 1327, 1397
HphI GGTGA 4 cut(s) 467, 579, 1066, 1087
Hpy166II GTNNAC 3 cut(s) 395, 829, 1323
Hpy188I TCNGA 4 cut(s) 67, 865, 1123, 1172
Hpy8I GTNNAC 3 cut(s) 395, 829, 1323
Hpy99I CGWCG 1 cut(s) 1341
HpyAV CCTTC 2 cut(s) 718, 1350
HpyCH4III ACNGT 5 cut(s) 372, 796, 872, 1341, 1364
HpyCH4IV ACGT 2 cut(s) 1089, 1415
HpyCH4V TGCA 8 cut(s) 158, 170, 325, 467, 736, 1268, 1323, 1419
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 3 cut(s) 15, 416, 785
HpySE526I ACGT 2 cut(s) 1089, 1415
Hsp92II CATG 6 cut(s) 179, 407, 464, 680, 745, 1187
HspAI GCGC 3 cut(s) 274, 441, 645
KspI CCGCGG 1 cut(s) 481
Kzo9I GATC 6 cut(s) 160, 508, 560, 892, 919, 1177
LmnI GCTCC 2 cut(s) 315, 1310
Lsp1109I GCAGC 3 cut(s) 83, 87, 451
LweI GCATC 1 cut(s) 334
MaeI CTAG 1 cut(s) 729
MaeII ACGT 2 cut(s) 1089, 1415
MaeIII GTNAC 1 cut(s) 30
MalI GATC 6 cut(s) 162, 510, 562, 894, 921, 1179
MbiI CCGCTC 1 cut(s) 702
MboI GATC 6 cut(s) 160, 508, 560, 892, 919, 1177
MfeI CAATTG 1 cut(s) 552
MflI RGATCY 3 cut(s) 160, 508, 919
MhlI GDGCHC 1 cut(s) 1325
MlyI GAGTC 2 cut(s) 1321, 1406
MmeI TCCRAC 1 cut(s) 1150
MnlI CCTC 7 cut(s) 136, 340, 391, 419, 550, 800, 1117
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 5 cut(s) 8, 230, 470, 780, 798
MslI CAYNNNNRTG 1 cut(s) 459
MspA1I CMGCKG 1 cut(s) 480
MspR9I CCNGG 1 cut(s) 201
MunI CAATTG 1 cut(s) 552
Mva1269I GAATGC 4 cut(s) 259, 647, 1021, 1268
MvaI CCWGG 1 cut(s) 201
MvnI CGCG 4 cut(s) 276, 441, 480, 1294
MwoI GCNNNNNNNGC 1 cut(s) 248
NdeII GATC 6 cut(s) 160, 508, 560, 892, 919, 1177
NlaIII CATG 6 cut(s) 179, 407, 464, 680, 745, 1187
NlaIV GGNNCC 2 cut(s) 21, 921
NruI TCGCGA 1 cut(s) 1294
NspI RCATGY 2 cut(s) 464, 1187
PaeR7I CTCGAG 1 cut(s) 1394
PagI TCATGA 1 cut(s) 676
PciI ACATGT 1 cut(s) 1183
PctI GAATGC 4 cut(s) 259, 647, 1021, 1268
PdmI GAANNNNTTC 1 cut(s) 288
PfeI GAWTC 5 cut(s) 117, 634, 656, 1003, 1217
PflMI CCANNNNNTGG 1 cut(s) 694
PkrI GCNGC 3 cut(s) 73, 102, 466
PleI GAGTC 2 cut(s) 1321, 1405
PpsI GAGTC 2 cut(s) 1321, 1405
Ppu21I YACGTR 1 cut(s) 1416
PpuMI RGGWCCY 1 cut(s) 19
PscI ACATGT 1 cut(s) 1183
PshBI ATTAAT 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 199
PspFI CCCAGC 1 cut(s) 251
PspGI CCWGG 1 cut(s) 199
PspN4I GGNNCC 2 cut(s) 21, 921
PspPI GGNCC 3 cut(s) 19, 451, 816
PspPPI RGGWCCY 1 cut(s) 19
PsuI RGATCY 3 cut(s) 160, 508, 919
RruI TCGCGA 1 cut(s) 1294
RsaI GTAC 6 cut(s) 135, 188, 874, 991, 1361, 1414
RsaNI GTAC 6 cut(s) 134, 187, 873, 990, 1360, 1413
RseI CAYNNNNRTG 1 cut(s) 459
SacII CCGCGG 1 cut(s) 481
SalI GTCGAC 1 cut(s) 393
SaqAI TTAA 5 cut(s) 8, 230, 470, 780, 798
SatI GCNGC 3 cut(s) 72, 101, 465
Sau3AI GATC 6 cut(s) 160, 508, 560, 892, 919, 1177
Sau96I GGNCC 3 cut(s) 19, 451, 816
ScaI AGTACT 1 cut(s) 874
SchI GAGTC 2 cut(s) 1321, 1406
ScrFI CCNGG 1 cut(s) 201
SduI GDGCHC 1 cut(s) 1325
SfaNI GCATC 1 cut(s) 334
Sfr274I CTCGAG 1 cut(s) 1394
Sfr303I CCGCGG 1 cut(s) 481
SgrBI CCGCGG 1 cut(s) 481
SinI GGWCC 1 cut(s) 19
SlaI CTCGAG 1 cut(s) 1394
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 2 cut(s) 512, 1394
SmoI CTYRAG 2 cut(s) 512, 1394
SsiI CCGC 7 cut(s) 431, 439, 478, 480, 668, 702, 1012
SspMI CTAG 1 cut(s) 729
StyD4I CCNGG 1 cut(s) 199
TaaI ACNGT 5 cut(s) 372, 796, 872, 1341, 1364
TaiI ACGT 2 cut(s) 1092, 1418
TaqI TCGA 4 cut(s) 394, 543, 1045, 1395
TatI WGTACW 2 cut(s) 186, 872
TfiI GAWTC 5 cut(s) 117, 634, 656, 1003, 1217
Tru1I TTAA 5 cut(s) 8, 230, 470, 780, 798
Tru9I TTAA 5 cut(s) 8, 230, 470, 780, 798
TscAI CASTG 2 cut(s) 838, 1014
TseI GCWGC 3 cut(s) 71, 100, 464
TspDTI ATGAA 7 cut(s) 63, 192, 665, 693, 1230, 1264, 1419
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 2 cut(s) 838, 1014
Van91I CCANNNNNTGG 1 cut(s) 694
VneI GTGCAC 1 cut(s) 1321
VpaK11BI GGWCC 1 cut(s) 19
VspI ATTAAT 1 cut(s) 470
XapI RAATTY 3 cut(s) 483, 1078, 1311
XceI RCATGY 2 cut(s) 464, 1187
XhoI CTCGAG 1 cut(s) 1394
XmiI GTMKAC 1 cut(s) 394
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 1 cut(s) 729
ZrmI AGTACT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.