Rroxscaffold_1G00044920

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000885
Physical Location & Seq
Forward (+)
63959060 .. 63969339
10280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00044920.1

Sequence Viewer

Length: 2001 bp
ATGGCTGCCAAAGCTTCGTGCTTTTCCTGCAAGGCCGAGAGTGATCAGTTCGTTAATGGCTGGGAGCTCAAGGATGGGTCTTTCGCCCAGCTCTGCAAGAAATGCGGTTTTGCTTATGTGAATGGTATATTTTGTGAAACTTTTCACATGGAAGTGGAAGGCTGGAGAGACTATGCTGTTTGTGGGAAGGAACAATCTCCAAGGGACATTGAAACAGGTCCAACACCTGTATATACAAGTCTGAAAGCAGACCAGGAACAATCTCCAAGGGACATTGAAACAGGTCCAACGCGTGTAGATACAACTCTGAAACCAGACCAGGAACAATCTCTAAGGGACTTTGAAACAGGTCCAACACCTGTAGATACAAGTCTGAAAACAGACCAGGAACAACCTCCAAAGGATATGGAGACAAGTCCAATGCCTGTAGAAACAAGTCATAAATCAGACCAGATAGCATTGCTAATAATAGGGACAATCTCCAAAGGATATGGAAACAGGTCCAACGCCTGTAGAAACAAGTCTGAAAACAGACCAGGTTCAACTCCTGAAGAAATAAATATGAAAACAGACCAGGAACAGTCTCCCAAGGGATATGGAAACAAGTCCGAAAACAGACTAGAAGCTCCAGAGACCCAAGGCCACCCACAAGCTTCCAGCAATACCCATGGGAATCCACAATCTTTGAAACCTCATGGGAATCCGGCCACAAGCTTGACGAAAGAATACTTCTCGGCCAACATGAAACGCCCATGGAAAAGAGAGTTCGAATTGCTGAATCGCAGTAGGGCATGGGAAGGTTCCGAAGGGGCATGGGGACGACGAAAAGCACTTCCACTTTCCGAGCTTCGTCGAGATATTGTATGTGAACTCCTTCCGAGTGTTACGAAATTTTTTTGGTTTGAACATTTCAACGGGGGACGGTTGCAAACCTTACAAGAGATGTATGGAACGTGTTTCGAAGTTCATCTATATGTTGTCACTAAAAGTTTGGCAGAGTCGGAGAGCGGATTGAATGACCTCTCAGATCTCCTCGAAGCCGTGAACAATAGTAGTAACGTAGAAAAAACAGTGTTGCCAATATTCTACGGTGTTGATCCCTCCGATGTACGACATCAAACAGGAAGTTTTGCAGAAGCCTTTGCCAACCATGAGAAAAATAAGTACAAGGATGATCCACAAAAAGTAGAAAGATGGAGAGCTGCTTTAAGGAAACTGGCAAATCTCGCTGGCTGGAATTCAAAGCACTGGCCTGAAGCAAAACTCAATCGAGAAGTTGTTGATGAGATATGGAGGAAATTGCATTCCTATTTTTCAAAACCTGCCATTAAAAATCTGGCATCAACACCTTCTTGGTCTTCTTCTTGTCGACGGAAGCATGATGTATTTCTAAGCTTTAGAGGGAAGGATACCCGCATGAATTTTTTGGACCATCTATATGATGCATTAATTCAGTGCAGAATTTCCACTTTTAGAGATGACAAAGATCTTGTTAAAGGAATGTCCATTCGCAAACAACTCGAAAAAGCAATTGAGGGATCAAAGGTTTGGCTTGTCATTGTTTCATCAAACTATGCTTCTTCAAAATGGTGCTTGGATGAGCTTTTGAAGATTCTTGAATGCAGAAAAGCATCTAAAGTGGGGAAGAATAGAAGTTTGGTGAAGGCATTTGCAGAATATGAAGAACAAGCTCATACTTTGGTGAATGGAGACAAAACACATAAGGGGAAGGCTAGTTTGCGGAACAAGAAAAACAGTACTAATCATAATAAAAGGAAAATGGGTGAGTTGAGACTTGGCGTTAATTATGGCGGAAAGTGGGTTGGTTCTGTCTACATCGCAGCCTTGCTACAAGGACTATCATCCATTCTTTCAGCAGTCGCAGTCTCAGCCTTCCTACATAGAGCCTCAGCCTCAGTACCAACAGCCGAATCCTTCCTACATGAAGCAGCCGCCGCCAATCCATCAAGTGCCTTACTCGCTCAAGATGTTCTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

666

Amino Acids

74.74

Weight (kDa)

8.65

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 325 - 442 8.7e-20 TIR domain
TIR PF01582 459 - 546 9.4e-30 TIR domain
TIR_2 PF13676 462 - 544 4.9e-14 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1330
AccBSI CCGCTC 1 cut(s) 1008
AccI GTMKAC 2 cut(s) 1369, 1833
AccII CGCG 1 cut(s) 292
AciI CCGC 7 cut(s) 105, 1008, 1414, 1741, 1812, 1953, 1956
AclWI GGATC 3 cut(s) 1091, 1169, 1546
AcoI YGGCCR 2 cut(s) 705, 735
AcsI RAATTY 4 cut(s) 890, 1237, 1420, 1461
AcuI CTGAAG 2 cut(s) 570, 1275
AfaI GTAC 4 cut(s) 1110, 1166, 1759, 1920
AflIII ACRYGT 2 cut(s) 290, 953
AjnI CCWGG 5 cut(s) 252, 318, 384, 535, 573
AjuI GAANNNNNNNTTGG 2 cut(s) 1640, 1672
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 7 cut(s) 162, 404, 588, 626, 1704, 1786, 1891
AlwI GGATC 3 cut(s) 1091, 1169, 1546
AoxI GGCC 5 cut(s) 33, 640, 705, 735, 1250
ApeKI GCWGC 4 cut(s) 5, 1202, 1841, 1949
ApoI RAATTY 4 cut(s) 890, 1237, 1420, 1461
AseI ATTAAT 1 cut(s) 1448
Asp700I GAANNNNTTC 2 cut(s) 141, 873
AspS9I GGNCC 5 cut(s) 218, 284, 350, 501, 1429
AsuHPI GGTGA 3 cut(s) 1672, 1714, 1796
AsuII TTCGAA 2 cut(s) 768, 960
AvaII GGWCC 5 cut(s) 218, 284, 350, 501, 1429
BanII GRGCYC 1 cut(s) 69
BbsI GAAGAC 1 cut(s) 1350
Bbv12I GWGCWC 1 cut(s) 69
BbvCI CCTCAGC 1 cut(s) 1909
BbvI GCAGC 3 cut(s) 1189, 1853, 1961
BccI CCATC 4 cut(s) 68, 1188, 1440, 1972
BceAI ACGGC 1 cut(s) 1025
BcgI CGANNNNNNTGC 2 cut(s) 1501, 1535
BciT130I CCWGG 5 cut(s) 254, 320, 386, 537, 575
BciVI GTATCC 1 cut(s) 1402
BclI TGATCA 1 cut(s) 43
BcoDI GTCTC 7 cut(s) 162, 404, 588, 626, 1704, 1786, 1891
BfaI CTAG 2 cut(s) 620, 1734
BfmI CTRYAG 3 cut(s) 360, 426, 511
BfuAI ACCTGC 1 cut(s) 1330
BfuI GTATCC 1 cut(s) 1402
BglII AGATCT 2 cut(s) 1027, 1486
BisI GCNGC 6 cut(s) 6, 1203, 1842, 1950, 1953, 1956
BlsI GCNGC 6 cut(s) 7, 1204, 1843, 1951, 1954, 1957
BmcAI AGTACT 1 cut(s) 1759
Bme1390I CCNGG 5 cut(s) 254, 320, 386, 537, 575
Bme18I GGWCC 5 cut(s) 218, 284, 350, 501, 1429
BmgT120I GGNCC 5 cut(s) 218, 284, 350, 501, 1429
BmiI GGNNCC 1 cut(s) 802
BmrFI CCNGG 5 cut(s) 254, 320, 386, 537, 575
BmsI GCATC 3 cut(s) 1349, 1432, 1640
BpiI GAAGAC 1 cut(s) 1350
BpmI CTGGAG 2 cut(s) 184, 612
Bpu10I CCTNAGC 1 cut(s) 1909
Bpu14I TTCGAA 2 cut(s) 768, 960
BpuEI CTTGAG 2 cut(s) 53, 1968
BsaI GGTCTC 1 cut(s) 626
BsaJI CCNNGG 6 cut(s) 200, 266, 588, 637, 667, 752
Bse1I ACTGG 2 cut(s) 1221, 1253
Bse3DI GCAATG 1 cut(s) 458
BseBI CCWGG 5 cut(s) 254, 320, 386, 537, 575
BseDI CCNNGG 6 cut(s) 200, 266, 588, 637, 667, 752
BseGI GGATG 4 cut(s) 79, 1177, 1603, 1862
BseMI GCAATG 1 cut(s) 458
BseMII CTCAG 4 cut(s) 1038, 1902, 1923, 1929
BseNI ACTGG 2 cut(s) 1221, 1253
BseRI GAGGAG 1 cut(s) 1022
BseXI GCAGC 3 cut(s) 1189, 1853, 1961
BseYI CCCAGC 2 cut(s) 60, 87
BsgI GTGCAG 1 cut(s) 1477
Bsh1236I CGCG 1 cut(s) 292
BshFI GGCC 5 cut(s) 35, 642, 707, 737, 1252
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 704
BslFI GGGAC 6 cut(s) 218, 284, 350, 487, 831, 933
BsmAI GTCTC 7 cut(s) 162, 404, 588, 626, 1704, 1786, 1891
BsmFI GGGAC 6 cut(s) 218, 284, 350, 487, 831, 933
BsmI GAATGC 2 cut(s) 1303, 1625
BsnI GGCC 5 cut(s) 35, 642, 707, 737, 1252
Bso31I GGTCTC 1 cut(s) 626
Bsp119I TTCGAA 2 cut(s) 768, 960
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 6 cut(s) 43, 1027, 1096, 1174, 1486, 1538
Bsp19I CCATGG 2 cut(s) 667, 752
BspACI CCGC 7 cut(s) 105, 1008, 1414, 1741, 1812, 1953, 1956
BspANI GGCC 5 cut(s) 35, 642, 707, 737, 1252
BspCNI CTCAG 4 cut(s) 1037, 1901, 1922, 1928
BspFNI CGCG 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 802
BspMI ACCTGC 1 cut(s) 1330
BspPI GGATC 3 cut(s) 1091, 1169, 1546
BspT104I TTCGAA 2 cut(s) 768, 960
BspTNI GGTCTC 1 cut(s) 626
BsrBI CCGCTC 1 cut(s) 1008
BsrDI GCAATG 1 cut(s) 458
BsrI ACTGG 2 cut(s) 1221, 1253
BssECI CCNNGG 6 cut(s) 200, 266, 588, 637, 667, 752
BssMI GATC 6 cut(s) 43, 1027, 1096, 1174, 1486, 1538
BssT1I CCWWGG 6 cut(s) 200, 266, 588, 637, 667, 752
Bst2UI CCWGG 5 cut(s) 254, 320, 386, 537, 575
Bst4CI ACNGT 5 cut(s) 582, 924, 1072, 1091, 1757
BstAPI GCANNNNNTGC 1 cut(s) 102
BstBI TTCGAA 2 cut(s) 768, 960
BstC8I GCNNGC 1 cut(s) 1231
BstDEI CTNAG 6 cut(s) 332, 1024, 1391, 1888, 1909, 1915
BstDSI CCRYGG 2 cut(s) 667, 752
BstF5I GGATG 4 cut(s) 79, 1177, 1603, 1862
BstFNI CGCG 1 cut(s) 292
BstKTI GATC 6 cut(s) 46, 1030, 1099, 1177, 1489, 1541
BstMAI GTCTC 7 cut(s) 162, 404, 588, 626, 1704, 1786, 1891
BstMBI GATC 6 cut(s) 43, 1027, 1096, 1174, 1486, 1538
BstMWI GCNNNNNNNGC 7 cut(s) 11, 27, 102, 1226, 1889, 1955, 1979
BstNI CCWGG 5 cut(s) 254, 320, 386, 537, 575
BstSCI CCNGG 5 cut(s) 252, 318, 384, 535, 573
BstSFI CTRYAG 3 cut(s) 360, 426, 511
BstUI CGCG 1 cut(s) 292
BstV1I GCAGC 3 cut(s) 1189, 1853, 1961
BstV2I GAAGAC 1 cut(s) 1350
BstX2I RGATCY 2 cut(s) 1027, 1486
BstYI RGATCY 2 cut(s) 1027, 1486
BsuI GTATCC 1 cut(s) 1402
BsuRI GGCC 5 cut(s) 35, 642, 707, 737, 1252
BtgI CCRYGG 2 cut(s) 667, 752
BtgZI GCGATG 1 cut(s) 1822
BtsCI GGATG 4 cut(s) 79, 1177, 1603, 1862
BtsIMutI CAGTG 3 cut(s) 1077, 1246, 1460
BveI ACCTGC 1 cut(s) 1330
Cac8I GCNNGC 1 cut(s) 1231
Cfr13I GGNCC 5 cut(s) 218, 284, 350, 501, 1429
CsiI ACCWGGT 1 cut(s) 535
Csp6I GTAC 4 cut(s) 1109, 1165, 1758, 1919
CspCI CAANNNNNGTGG 2 cut(s) 666, 701
CviQI GTAC 4 cut(s) 1109, 1165, 1758, 1919
DdeI CTNAG 6 cut(s) 332, 1024, 1391, 1888, 1909, 1915
DpnI GATC 6 cut(s) 45, 1029, 1098, 1176, 1488, 1540
DpnII GATC 6 cut(s) 43, 1027, 1096, 1174, 1486, 1538
EaeI YGGCCR 2 cut(s) 705, 735
EciI GGCGGA 1 cut(s) 1827
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 6 cut(s) 200, 266, 588, 637, 667, 752
Eco24I GRGCYC 1 cut(s) 69
Eco31I GGTCTC 1 cut(s) 626
Eco47I GGWCC 5 cut(s) 218, 284, 350, 501, 1429
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 2 cut(s) 570, 1275
EcoICRI GAGCTC 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 1237
EcoRII CCWGG 5 cut(s) 252, 318, 384, 535, 573
EcoT14I CCWWGG 6 cut(s) 200, 266, 588, 637, 667, 752
EcoT22I ATGCAT 1 cut(s) 1447
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 6 cut(s) 200, 266, 588, 637, 667, 752
FaqI GGGAC 6 cut(s) 218, 284, 350, 487, 831, 933
FauI CCCGC 1 cut(s) 1421
FbaI TGATCA 1 cut(s) 43
FblI GTMKAC 2 cut(s) 1369, 1833
Fnu4HI GCNGC 6 cut(s) 6, 1203, 1842, 1950, 1953, 1956
FokI GGATG 4 cut(s) 86, 1184, 1610, 1849
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 6 cut(s) 6, 1203, 1842, 1950, 1953, 1956
FspBI CTAG 2 cut(s) 620, 1734
GluI GCNGC 6 cut(s) 6, 1203, 1842, 1950, 1953, 1956
GsaI CCCAGC 2 cut(s) 64, 91
GsuI CTGGAG 2 cut(s) 184, 612
HaeIII GGCC 5 cut(s) 35, 642, 707, 737, 1252
HapII CCGG 1 cut(s) 704
HincII GTYRAC 1 cut(s) 1370
HindII GTYRAC 1 cut(s) 1370
HindIII AAGCTT 4 cut(s) 12, 651, 712, 1393
HinfI GANTC 6 cut(s) 673, 700, 778, 998, 1612, 1931
HpaII CCGG 1 cut(s) 704
HphI GGTGA 3 cut(s) 1672, 1714, 1796
Hpy166II GTNNAC 4 cut(s) 869, 1045, 1370, 1834
Hpy188III TCNNGA 7 cut(s) 548, 629, 854, 1271, 1616, 1985, 1994
Hpy8I GTNNAC 4 cut(s) 869, 1045, 1370, 1834
Hpy99I CGWCG 3 cut(s) 825, 855, 1374
HpyCH4III ACNGT 5 cut(s) 582, 924, 1072, 1091, 1757
HpyCH4IV ACGT 2 cut(s) 953, 1059
HpyCH4V TGCA 9 cut(s) 30, 96, 928, 1133, 1303, 1445, 1458, 1623, 1673
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 27, 102, 1226, 1889, 1955, 1979
HpyF3I CTNAG 6 cut(s) 332, 1024, 1391, 1888, 1909, 1915
HpySE526I ACGT 2 cut(s) 953, 1059
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 6 cut(s) 43, 1027, 1096, 1174, 1486, 1538
LmnI GCTCC 2 cut(s) 64, 631
Lsp1109I GCAGC 3 cut(s) 1189, 1853, 1961
LweI GCATC 3 cut(s) 1349, 1432, 1640
MabI ACCWGGT 1 cut(s) 535
MaeI CTAG 2 cut(s) 620, 1734
MaeII ACGT 2 cut(s) 953, 1059
MaeIII GTNAC 3 cut(s) 883, 979, 1055
MalI GATC 6 cut(s) 45, 1029, 1098, 1176, 1488, 1540
MbiI CCGCTC 1 cut(s) 1008
MboI GATC 6 cut(s) 43, 1027, 1096, 1174, 1486, 1538
MboII GAAGA 7 cut(s) 563, 1350, 1353, 1572, 1621, 1657, 1694
MfeI CAATTG 1 cut(s) 1530
MflI RGATCY 2 cut(s) 1027, 1486
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 9 cut(s) 770, 890, 1237, 1298, 1420, 1449, 1461, 1530, 1804
MluI ACGCGT 1 cut(s) 290
MlyI GAGTC 1 cut(s) 1007
MmeI TCCRAC 5 cut(s) 245, 311, 377, 528, 981
Mph1103I ATGCAT 1 cut(s) 1447
MroXI GAANNNNTTC 2 cut(s) 141, 873
MseI TTAA 6 cut(s) 54, 1208, 1329, 1448, 1494, 1803
MslI CAYNNNNRTG 3 cut(s) 152, 972, 1437
MspI CCGG 1 cut(s) 704
MspR9I CCNGG 5 cut(s) 254, 320, 386, 537, 575
MunI CAATTG 1 cut(s) 1530
Mva1269I GAATGC 2 cut(s) 1303, 1625
MvaI CCWGG 5 cut(s) 254, 320, 386, 537, 575
MvnI CGCG 1 cut(s) 292
MwoI GCNNNNNNNGC 7 cut(s) 11, 27, 102, 1226, 1889, 1955, 1979
NcoI CCATGG 2 cut(s) 667, 752
NdeII GATC 6 cut(s) 43, 1027, 1096, 1174, 1486, 1538
NlaIV GGNNCC 1 cut(s) 802
NmeAIII GCCGAG 2 cut(s) 61, 713
NmuCI GTSAC 1 cut(s) 979
NsiI ATGCAT 1 cut(s) 1447
NspV TTCGAA 2 cut(s) 768, 960
PctI GAATGC 2 cut(s) 1303, 1625
PdmI GAANNNNTTC 2 cut(s) 141, 873
PfeI GAWTC 5 cut(s) 673, 700, 778, 1612, 1931
PkrI GCNGC 6 cut(s) 7, 1204, 1843, 1951, 1954, 1957
PleI GAGTC 1 cut(s) 1006
PpsI GAGTC 1 cut(s) 1006
PshBI ATTAAT 1 cut(s) 1448
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 5 cut(s) 252, 318, 384, 535, 573
PspFI CCCAGC 2 cut(s) 60, 87
PspGI CCWGG 5 cut(s) 252, 318, 384, 535, 573
PspN4I GGNNCC 1 cut(s) 802
PspPI GGNCC 5 cut(s) 218, 284, 350, 501, 1429
PsuI RGATCY 2 cut(s) 1027, 1486
RsaI GTAC 4 cut(s) 1110, 1166, 1759, 1920
RsaNI GTAC 4 cut(s) 1109, 1165, 1758, 1919
RseI CAYNNNNRTG 3 cut(s) 152, 972, 1437
SacI GAGCTC 1 cut(s) 69
SalI GTCGAC 1 cut(s) 1368
SaqAI TTAA 6 cut(s) 54, 1208, 1329, 1448, 1494, 1803
SatI GCNGC 6 cut(s) 6, 1203, 1842, 1950, 1953, 1956
Sau3AI GATC 6 cut(s) 43, 1027, 1096, 1174, 1486, 1538
Sau96I GGNCC 5 cut(s) 218, 284, 350, 501, 1429
ScaI AGTACT 1 cut(s) 1759
SchI GAGTC 1 cut(s) 1007
ScrFI CCNGG 5 cut(s) 254, 320, 386, 537, 575
SduI GDGCHC 1 cut(s) 69
SexAI ACCWGGT 1 cut(s) 535
SfaNI GCATC 3 cut(s) 1349, 1432, 1640
SfcI CTRYAG 3 cut(s) 360, 426, 511
SfuI TTCGAA 2 cut(s) 768, 960
SinI GGWCC 5 cut(s) 218, 284, 350, 501, 1429
SmiMI CAYNNNNRTG 3 cut(s) 152, 972, 1437
SmlI CTYRAG 2 cut(s) 68, 1983
SmoI CTYRAG 2 cut(s) 68, 1983
Sse9I AATT 9 cut(s) 770, 890, 1237, 1298, 1420, 1449, 1461, 1530, 1804
SsiI CCGC 7 cut(s) 105, 1008, 1414, 1741, 1812, 1953, 1956
SspI AATATT 1 cut(s) 1083
SspMI CTAG 2 cut(s) 620, 1734
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 5 cut(s) 252, 318, 384, 535, 573
StyI CCWWGG 6 cut(s) 200, 266, 588, 637, 667, 752
TaaI ACNGT 5 cut(s) 582, 924, 1072, 1091, 1757
TaiI ACGT 2 cut(s) 956, 1062
TaqI TCGA 7 cut(s) 768, 853, 960, 1035, 1270, 1369, 1521
TasI AATT 9 cut(s) 770, 890, 1237, 1298, 1420, 1449, 1461, 1530, 1804
TatI WGTACW 2 cut(s) 1164, 1757
TauI GCSGC 2 cut(s) 1955, 1958
TfiI GAWTC 5 cut(s) 673, 700, 778, 1612, 1931
Tru1I TTAA 6 cut(s) 54, 1208, 1329, 1448, 1494, 1803
Tru9I TTAA 6 cut(s) 54, 1208, 1329, 1448, 1494, 1803
TscAI CASTG 3 cut(s) 1077, 1253, 1460
TseFI GTSAC 1 cut(s) 979
TseI GCWGC 4 cut(s) 5, 1202, 1841, 1949
Tsp45I GTSAC 1 cut(s) 979
TspDTI ATGAA 7 cut(s) 578, 758, 956, 1433, 1554, 1695, 1959
TspGWI ACGGA 1 cut(s) 1387
TspRI CASTG 3 cut(s) 1077, 1253, 1460
VpaK11BI GGWCC 5 cut(s) 218, 284, 350, 501, 1429
VspI ATTAAT 1 cut(s) 1448
XapI RAATTY 4 cut(s) 890, 1237, 1420, 1461
XcmI CCANNNNNNNNNTGG 1 cut(s) 1333
XmiI GTMKAC 2 cut(s) 1369, 1833
XmnI GAANNNNTTC 2 cut(s) 141, 873
XspI CTAG 2 cut(s) 620, 1734
ZrmI AGTACT 1 cut(s) 1759
Zsp2I ATGCAT 1 cut(s) 1447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.