Rmu_sc0001493.1_g000077

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001493.1
OG0000885
Physical Location & Seq
Forward (+)
289790 .. 290643
854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001493.1_g000077.1.cds

Sequence Viewer

Length: 408 bp
atgctagctgctgatatatatgttgtcactcaaagtttggcacggttggagagggaattggatgacctctcggagcttctcgaagagaaaacagcgctgccaatattctacggtgttgatccctccgatgtacgacatcaaacaggaagttttgcacaagcctttgcagaccatgagataaagtataaggatgacccacaaaaagtaaaaaggtggagagctgctttaacgaaactggcaaatccgctggctgggattcaaagggttggtgatcagtggccgggcgctgactccagtgaccggaatccagggacaggtgaccggaatcgggcgaccggtcacctgaatcaggaatctggctaccagacaggtgtccggattcagacgtctgaatttattgcctcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

135

Amino Acids

15.0

Weight (kDa)

5.32

Isoelectric Point (pI)

27.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 389
AccIII TCCGGA 1 cut(s) 375
AciI CCGC 1 cut(s) 245
AclWI GGATC 1 cut(s) 113
AcoI YGGCCR 1 cut(s) 278
AcsI RAATTY 1 cut(s) 392
AcyI GRCGYC 1 cut(s) 386
AfaI GTAC 1 cut(s) 132
AfeI AGCGCT 1 cut(s) 96
AfiI CCNNNNNNNGG 5 cut(s) 251, 300, 314, 328, 349
AgeI ACCGGT 1 cut(s) 335
AgsI TTSAA 1 cut(s) 260
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 3 cut(s) 8, 76, 221
AluI AGCT 3 cut(s) 8, 76, 221
AlwI GGATC 1 cut(s) 113
Aor13HI TCCGGA 1 cut(s) 375
Aor51HI AGCGCT 1 cut(s) 96
AoxI GGCC 1 cut(s) 278
ApeKI GCWGC 3 cut(s) 8, 97, 221
ApoI RAATTY 1 cut(s) 392
AsiGI ACCGGT 1 cut(s) 335
AspLEI GCGC 2 cut(s) 97, 287
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 3 cut(s) 281, 329, 332
AsuNHI GCTAGC 1 cut(s) 4
BbvI GCAGC 2 cut(s) 84, 208
BciT130I CCWGG 1 cut(s) 309
BclI TGATCA 1 cut(s) 271
BcnI CCSGG 1 cut(s) 282
BfaI CTAG 1 cut(s) 5
BfoI RGCGCY 2 cut(s) 98, 288
BisI GCNGC 3 cut(s) 9, 98, 222
BlsI GCNGC 3 cut(s) 10, 99, 223
Bme1390I CCNGG 2 cut(s) 282, 309
BmrFI CCNGG 2 cut(s) 282, 309
BmtI GCTAGC 1 cut(s) 8
BoxI GACNNNNGTC 1 cut(s) 371
BpmI CTGGAG 1 cut(s) 277
BpuMI CCSGG 1 cut(s) 282
BsaHI GRCGYC 1 cut(s) 386
BsaJI CCNNGG 1 cut(s) 308
BsaWI WCCGGW 4 cut(s) 300, 321, 335, 375
Bsc4I CCNNNNNNNGG 5 cut(s) 251, 300, 314, 328, 349
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 2 cut(s) 240, 294
BseAI TCCGGA 1 cut(s) 375
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 308
BseGI GGATG 2 cut(s) 67, 196
BseLI CCNNNNNNNGG 5 cut(s) 251, 300, 314, 328, 349
BseNI ACTGG 2 cut(s) 240, 294
BseXI GCAGC 2 cut(s) 84, 208
BseYI CCCAGC 1 cut(s) 251
Bsh1285I CGRYCG 1 cut(s) 336
BshFI GGCC 1 cut(s) 280
BshTI ACCGGT 1 cut(s) 335
BsiEI CGRYCG 1 cut(s) 336
BsiSI CCGG 5 cut(s) 281, 301, 322, 336, 376
BslFI GGGAC 1 cut(s) 325
BslI CCNNNNNNNGG 5 cut(s) 251, 300, 314, 328, 349
BsmFI GGGAC 1 cut(s) 325
BsnI GGCC 1 cut(s) 280
Bsp13I TCCGGA 1 cut(s) 375
Bsp143I GATC 2 cut(s) 118, 271
BspACI CCGC 1 cut(s) 245
BspANI GGCC 1 cut(s) 280
BspEI TCCGGA 1 cut(s) 375
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 1 cut(s) 113
BsrFI RCCGGY 1 cut(s) 335
BsrI ACTGG 2 cut(s) 240, 294
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 2 cut(s) 118, 271
BssNI GRCGYC 1 cut(s) 386
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 2 cut(s) 45, 113
Bst6I CTCTTC 1 cut(s) 78
BstACI GRCGYC 1 cut(s) 386
BstC8I GCNNGC 2 cut(s) 6, 249
BstEII GGTNACC 2 cut(s) 317, 338
BstENI CCTNNNNNAGG 1 cut(s) 347
BstF5I GGATG 2 cut(s) 67, 196
BstH2I RGCGCY 2 cut(s) 98, 288
BstHHI GCGC 2 cut(s) 97, 287
BstKTI GATC 2 cut(s) 121, 274
BstMBI GATC 2 cut(s) 118, 271
BstMCI CGRYCG 1 cut(s) 336
BstNI CCWGG 1 cut(s) 309
BstPAI GACNNNNGTC 1 cut(s) 371
BstPI GGTNACC 2 cut(s) 317, 338
BstSCI CCNGG 2 cut(s) 280, 307
BstV1I GCAGC 2 cut(s) 84, 208
BsuRI GGCC 1 cut(s) 280
BtsCI GGATG 2 cut(s) 67, 196
BtsIMutI CAGTG 2 cut(s) 281, 301
Cac8I GCNNGC 2 cut(s) 6, 249
CfoI GCGC 2 cut(s) 97, 287
Cfr10I RCCGGY 1 cut(s) 335
Csp6I GTAC 1 cut(s) 131
CspAI ACCGGT 1 cut(s) 335
CviAII CATG 1 cut(s) 173
CviJI RGCY 7 cut(s) 8, 76, 161, 221, 251, 280, 360
CviKI_1 RGCY 7 cut(s) 8, 76, 161, 221, 251, 280, 360
CviQI GTAC 1 cut(s) 131
DpnI GATC 2 cut(s) 120, 273
DpnII GATC 2 cut(s) 118, 271
EaeI YGGCCR 1 cut(s) 278
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco47III AGCGCT 1 cut(s) 96
Eco91I GGTNACC 2 cut(s) 317, 338
EcoNI CCTNNNNNAGG 1 cut(s) 347
EcoO65I GGTNACC 2 cut(s) 317, 338
EcoRII CCWGG 1 cut(s) 307
FaeI CATG 1 cut(s) 176
FaiI YATR 5 cut(s) 17, 19, 21, 174, 186
FaqI GGGAC 1 cut(s) 325
FatI CATG 1 cut(s) 172
FbaI TGATCA 1 cut(s) 271
Fnu4HI GCNGC 3 cut(s) 9, 98, 222
FokI GGATG 2 cut(s) 74, 203
Fsp4HI GCNGC 3 cut(s) 9, 98, 222
FspBI CTAG 1 cut(s) 5
GlaI GCGC 2 cut(s) 96, 286
GluI GCNGC 3 cut(s) 9, 98, 222
GsaI CCCAGC 1 cut(s) 255
GsuI CTGGAG 1 cut(s) 277
HaeII RGCGCY 2 cut(s) 98, 288
HaeIII GGCC 1 cut(s) 280
HapII CCGG 5 cut(s) 281, 301, 322, 336, 376
HhaI GCGC 2 cut(s) 97, 287
Hin1I GRCGYC 1 cut(s) 386
Hin1II CATG 1 cut(s) 176
Hin6I GCGC 2 cut(s) 95, 285
HinP1I GCGC 2 cut(s) 95, 285
HinfI GANTC 7 cut(s) 256, 290, 304, 325, 346, 353, 379
HpaII CCGG 5 cut(s) 281, 301, 322, 336, 376
HphI GGTGA 3 cut(s) 281, 329, 332
Hpy188I TCNGA 4 cut(s) 73, 127, 384, 391
Hpy188III TCNNGA 3 cut(s) 80, 350, 376
HpyCH4III ACNGT 2 cut(s) 45, 113
HpyCH4IV ACGT 1 cut(s) 386
HpyCH4V TGCA 2 cut(s) 155, 167
HpySE526I ACGT 1 cut(s) 386
Hsp92I GRCGYC 1 cut(s) 386
Hsp92II CATG 1 cut(s) 176
HspAI GCGC 2 cut(s) 95, 285
Kpn2I TCCGGA 1 cut(s) 375
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 2 cut(s) 118, 271
LmnI GCTCC 1 cut(s) 73
Lsp1109I GCAGC 2 cut(s) 84, 208
MaeI CTAG 1 cut(s) 5
MaeII ACGT 1 cut(s) 386
MaeIII GTNAC 4 cut(s) 25, 296, 317, 338
MalI GATC 2 cut(s) 120, 273
MboI GATC 2 cut(s) 118, 271
MboII GAAGA 1 cut(s) 95
MluCI AATT 2 cut(s) 56, 392
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 1 cut(s) 27
MnlI CCTC 3 cut(s) 45, 77, 133
MroI TCCGGA 1 cut(s) 375
MseI TTAA 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 247
MspI CCGG 5 cut(s) 281, 301, 322, 336, 376
MspR9I CCNGG 2 cut(s) 282, 309
MvaI CCWGG 1 cut(s) 309
NciI CCSGG 1 cut(s) 282
NdeII GATC 2 cut(s) 118, 271
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 1 cut(s) 176
NmuCI GTSAC 4 cut(s) 25, 296, 317, 338
PfeI GAWTC 6 cut(s) 256, 304, 325, 346, 353, 379
PinAI ACCGGT 1 cut(s) 335
PkrI GCNGC 3 cut(s) 10, 99, 223
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
PshAI GACNNNNGTC 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 307
PspEI GGTNACC 2 cut(s) 317, 338
PspFI CCCAGC 1 cut(s) 251
PspGI CCWGG 1 cut(s) 307
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 227
SatI GCNGC 3 cut(s) 9, 98, 222
Sau3AI GATC 2 cut(s) 118, 271
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 2 cut(s) 282, 309
SetI ASST 9 cut(s) 10, 69, 78, 215, 223, 319, 345, 373, 389
Sse9I AATT 2 cut(s) 56, 392
SsiI CCGC 1 cut(s) 245
SspI AATATT 1 cut(s) 105
SspMI CTAG 1 cut(s) 5
StyD4I CCNGG 2 cut(s) 280, 307
TaaI ACNGT 2 cut(s) 45, 113
TaiI ACGT 1 cut(s) 389
TaqI TCGA 1 cut(s) 81
TasI AATT 2 cut(s) 56, 392
TfiI GAWTC 6 cut(s) 256, 304, 325, 346, 353, 379
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TscAI CASTG 2 cut(s) 281, 301
TseFI GTSAC 4 cut(s) 25, 296, 317, 338
TseI GCWGC 3 cut(s) 8, 97, 221
Tsp45I GTSAC 4 cut(s) 25, 296, 317, 338
TspRI CASTG 2 cut(s) 281, 301
XagI CCTNNNNNAGG 1 cut(s) 347
XapI RAATTY 1 cut(s) 392
XspI CTAG 1 cut(s) 5
ZraI GACGTC 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.