FvH4_3g20962

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000885
Physical Location & Seq
Reverse (-)
13997118 .. 13998440
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20962.t1

Sequence Viewer

Length: 1092 bp
ATGACGTCCGATGGTCCATCCACAGACTCATGGGACGAAGTGTTTGTATGGGGGAGTTCAAACACTATATGGTATGAAGGTTTTTCCAAAGACTTGGATTTAGACTCAACATACTCGAGCTTCATAGTCTCATCGGAGTACCCAAGCTTCATAGACCCCTGGGAGTCGACAAGCTTCGTAGACTTGTTGGAATATCCAGGCTTTAATCGCTTTATAGACCCATTGGAATGCTCAAGCTTCATAGAACAATTGGAATCCTCACCAGGCTTCAAAGCTGCGTGGAAATCCAAATGGGAATCCATCTTCGTCGACTTATTGGAACGCCCATGCTTCAACATATTATGTGAACGCCCATGCTTCAAAGCCAAATGGGAGTCCAAATGGAAACAAGAGTTCGAGTCGCTGAAGCAAAACAGTGCATGGGGAGAGGCATGGGCACGACGAAAAGCACTTCCACTTTCCGAGCTTCCGAGTATTGACAAACAACCTGGATCGTTTCGCTTTCCCAAGGATCCCTCAATGCTCATAAGAACTGGTCCACATATGCATTTGTACCTGCAGGAACGGTGGAAAAAGTTGGACGAAGTTCATCTAGATGTTGTGACCAAAAGTTTGGCACGGTCGGAGAGGGCATTGGATGACCTCGTCGAGCTTGTCCAAAAATCGAATGATGTAGGAAAAACAGTGCTGCCGATAATATTCTACGGTGTCGATCCTTCCGATGTACCAAATCAAACTGGAAGTTTTGCAGAAGCCTTTGCCTACTATGACAAGAAGTACAAGGATGAACCAGGAAAAGTAGAAAGGTGGAGAGCTGCTTTAAGGAAACTGGGAAATCTCGCTGGCTGGGATTCAAAGCACTGGACTGAAGGAAAACTCATTCAAGATGTTGTTGACGAGACACGGAGGATATTGTGTTCCTCTTTCTCAAAACCTCCCGAAAAAATTATGTCATCCACACCTTCTTGGTCTTTTTCTTGTCGACAGAAGCATGATGTATTTCTGAGCTTTAGAGGGAAGGATACCCGCTTGAATTTTTTGGACCATCTATATGATCATTATGATAATTCAGTGCAGAATTTCCACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

364

Amino Acids

42.17

Weight (kDa)

5.51

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 200 - 310 2.3e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 564
AccI GTMKAC 4 cut(s) 167, 180, 309, 982
AciI CCGC 1 cut(s) 1027
AclWI GGATC 4 cut(s) 499, 506, 519, 707
AcsI RAATTY 2 cut(s) 1033, 1078
AcuI CTGAAG 2 cut(s) 425, 888
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 140, 554, 726, 779
AgsI TTSAA 7 cut(s) 60, 271, 334, 361, 855, 884, 1033
AjnI CCWGG 5 cut(s) 158, 196, 262, 487, 790
AloI GAACNNNNNNTCC 2 cut(s) 901, 933
AluBI AGCT 9 cut(s) 120, 147, 174, 237, 275, 466, 652, 815, 1008
AluI AGCT 9 cut(s) 120, 147, 174, 237, 275, 466, 652, 815, 1008
Alw26I GTCTC 2 cut(s) 133, 893
AlwI GGATC 4 cut(s) 499, 506, 519, 707
Ama87I CYCGRG 1 cut(s) 115
ApeKI GCWGC 3 cut(s) 275, 688, 815
ApoI RAATTY 2 cut(s) 1033, 1078
ArsI GACNNNNNNTTYG 4 cut(s) 26, 58, 595, 627
AspS9I GGNCC 3 cut(s) 14, 536, 1042
AsuHPI GGTGA 1 cut(s) 252
AvaI CYCGRG 1 cut(s) 115
AvaII GGWCC 3 cut(s) 14, 536, 1042
BaeGI GKGCMC 1 cut(s) 439
BamHI GGATCC 1 cut(s) 511
BarI GAAGNNNNNNTAC 2 cut(s) 131, 163
BbvI GCAGC 3 cut(s) 262, 675, 802
BccI CCATC 4 cut(s) 5, 25, 308, 1053
BciT130I CCWGG 5 cut(s) 160, 198, 264, 489, 792
BciVI GTATCC 1 cut(s) 1015
BclI TGATCA 1 cut(s) 1054
BcoDI GTCTC 2 cut(s) 133, 893
BfaI CTAG 1 cut(s) 593
BfmI CTRYAG 1 cut(s) 557
BfuAI ACCTGC 1 cut(s) 564
BfuI GTATCC 1 cut(s) 1015
BisI GCNGC 3 cut(s) 276, 689, 816
BlsI GCNGC 3 cut(s) 277, 690, 817
Bme1390I CCNGG 5 cut(s) 160, 198, 264, 489, 792
Bme18I GGWCC 3 cut(s) 14, 536, 1042
BmeT110I CYCGRG 1 cut(s) 115
BmgT120I GGNCC 3 cut(s) 14, 536, 1042
BmiI GGNNCC 1 cut(s) 513
BmrFI CCNGG 5 cut(s) 160, 198, 264, 489, 792
BmrI ACTGGG 1 cut(s) 839
BmuI ACTGGG 1 cut(s) 839
BpuEI CTTGAG 1 cut(s) 217
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 158, 159, 507
Bse1I ACTGG 4 cut(s) 538, 742, 834, 866
BseBI CCWGG 5 cut(s) 160, 198, 264, 489, 792
BseDI CCNNGG 3 cut(s) 158, 159, 507
BseGI GGATG 4 cut(s) 17, 643, 790, 953
BseMII CTCAG 1 cut(s) 995
BseNI ACTGG 4 cut(s) 538, 742, 834, 866
BseSI GKGCMC 1 cut(s) 439
BseXI GCAGC 3 cut(s) 262, 675, 802
BseYI CCCAGC 1 cut(s) 846
Bsh1285I CGRYCG 1 cut(s) 623
BsiEI CGRYCG 1 cut(s) 623
BsiHKCI CYCGRG 1 cut(s) 115
BslFI GGGAC 1 cut(s) 47
BsmAI GTCTC 2 cut(s) 133, 893
BsmFI GGGAC 1 cut(s) 47
BsmI GAATGC 1 cut(s) 233
BsoBI CYCGRG 1 cut(s) 115
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 4 cut(s) 491, 511, 712, 1054
BspACI CCGC 1 cut(s) 1027
BspCNI CTCAG 1 cut(s) 996
BspLI GGNNCC 1 cut(s) 513
BspMAI CTGCAG 1 cut(s) 561
BspMI ACCTGC 1 cut(s) 564
BspPI GGATC 4 cut(s) 499, 506, 519, 707
BsrI ACTGG 4 cut(s) 538, 742, 834, 866
BssECI CCNNGG 3 cut(s) 158, 159, 507
BssMI GATC 4 cut(s) 491, 511, 712, 1054
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 507
Bst2UI CCWGG 5 cut(s) 160, 198, 264, 489, 792
Bst4CI ACNGT 5 cut(s) 416, 567, 621, 685, 707
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 844
BstDEI CTNAG 1 cut(s) 1004
BstF5I GGATG 4 cut(s) 17, 643, 790, 953
BstKTI GATC 4 cut(s) 494, 514, 715, 1057
BstMAI GTCTC 2 cut(s) 133, 893
BstMBI GATC 4 cut(s) 491, 511, 712, 1054
BstMCI CGRYCG 1 cut(s) 623
BstMWI GCNNNNNNNGC 1 cut(s) 207
BstNI CCWGG 5 cut(s) 160, 198, 264, 489, 792
BstSCI CCNGG 5 cut(s) 158, 196, 262, 487, 790
BstSFI CTRYAG 1 cut(s) 557
BstSLI GKGCMC 1 cut(s) 439
BstV1I GCAGC 3 cut(s) 262, 675, 802
BstX2I RGATCY 1 cut(s) 511
BstXI CCANNNNNNTGG 2 cut(s) 94, 613
BstYI RGATCY 1 cut(s) 511
BsuI GTATCC 1 cut(s) 1015
BtsCI GGATG 4 cut(s) 17, 643, 790, 953
BtsIMutI CAGTG 4 cut(s) 421, 690, 859, 1077
BveI ACCTGC 1 cut(s) 564
Cac8I GCNNGC 1 cut(s) 844
Cfr13I GGNCC 3 cut(s) 14, 536, 1042
Csp6I GTAC 4 cut(s) 139, 553, 725, 778
CviAII CATG 6 cut(s) 30, 327, 354, 420, 432, 992
CviQI GTAC 4 cut(s) 139, 553, 725, 778
DdeI CTNAG 1 cut(s) 1004
DpnI GATC 4 cut(s) 493, 513, 714, 1056
DpnII GATC 4 cut(s) 491, 511, 712, 1054
Eco130I CCWWGG 1 cut(s) 507
Eco47I GGWCC 3 cut(s) 14, 536, 1042
Eco57I CTGAAG 2 cut(s) 425, 888
Eco88I CYCGRG 1 cut(s) 115
EcoRII CCWGG 5 cut(s) 158, 196, 262, 487, 790
EcoT14I CCWWGG 1 cut(s) 507
EcoT22I ATGCAT 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 6 cut(s) 33, 330, 357, 423, 435, 995
FaqI GGGAC 1 cut(s) 47
FatI CATG 6 cut(s) 29, 326, 353, 419, 431, 991
FauI CCCGC 1 cut(s) 1034
FauNDI CATATG 1 cut(s) 543
FbaI TGATCA 1 cut(s) 1054
FblI GTMKAC 4 cut(s) 167, 180, 309, 982
Fnu4HI GCNGC 3 cut(s) 276, 689, 816
FokI GGATG 4 cut(s) 4, 650, 797, 940
Fsp4HI GCNGC 3 cut(s) 276, 689, 816
FspBI CTAG 1 cut(s) 593
GluI GCNGC 3 cut(s) 276, 689, 816
GsaI CCCAGC 1 cut(s) 850
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 33, 330, 357, 423, 435, 995
HincII GTYRAC 4 cut(s) 168, 310, 895, 983
HindII GTYRAC 4 cut(s) 168, 310, 895, 983
HindIII AAGCTT 3 cut(s) 145, 172, 235
HinfI GANTC 8 cut(s) 26, 104, 164, 254, 296, 374, 398, 851
HphI GGTGA 1 cut(s) 252
Hpy166II GTNNAC 7 cut(s) 168, 181, 310, 347, 539, 895, 983
Hpy188I TCNGA 7 cut(s) 10, 136, 463, 471, 625, 721, 1005
Hpy188III TCNNGA 3 cut(s) 593, 884, 938
Hpy8I GTNNAC 7 cut(s) 168, 181, 310, 347, 539, 895, 983
Hpy99I CGWCG 3 cut(s) 311, 444, 650
HpyAV CCTTC 5 cut(s) 71, 726, 863, 972, 1012
HpyCH4III ACNGT 5 cut(s) 416, 567, 621, 685, 707
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 5 cut(s) 419, 547, 559, 749, 1075
HpyF10VI GCNNNNNNNGC 1 cut(s) 207
HpyF3I CTNAG 1 cut(s) 1004
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 33, 330, 357, 423, 435, 995
Ksp22I TGATCA 1 cut(s) 1054
Kzo9I GATC 4 cut(s) 491, 511, 712, 1054
Lsp1109I GCAGC 3 cut(s) 262, 675, 802
MaeI CTAG 1 cut(s) 593
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 1 cut(s) 601
MalI GATC 4 cut(s) 493, 513, 714, 1056
MboI GATC 4 cut(s) 491, 511, 712, 1054
MboII GAAGA 1 cut(s) 295
MfeI CAATTG 1 cut(s) 248
MflI RGATCY 1 cut(s) 511
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 5 cut(s) 248, 945, 1033, 1066, 1078
MlyI GAGTC 5 cut(s) 20, 98, 173, 383, 407
MmeI TCCRAC 3 cut(s) 168, 558, 603
MnlI CCTC 9 cut(s) 268, 421, 526, 621, 653, 900, 931, 945, 1007
Mph1103I ATGCAT 1 cut(s) 549
MseI TTAA 2 cut(s) 204, 821
MslI CAYNNNNRTG 3 cut(s) 226, 594, 1050
MspR9I CCNGG 5 cut(s) 160, 198, 264, 489, 792
MunI CAATTG 1 cut(s) 248
Mva1269I GAATGC 1 cut(s) 233
MvaI CCWGG 5 cut(s) 160, 198, 264, 489, 792
MwoI GCNNNNNNNGC 1 cut(s) 207
NdeI CATATG 1 cut(s) 543
NdeII GATC 4 cut(s) 491, 511, 712, 1054
NlaIII CATG 6 cut(s) 33, 330, 357, 423, 435, 995
NlaIV GGNNCC 1 cut(s) 513
NmuCI GTSAC 1 cut(s) 601
NsiI ATGCAT 1 cut(s) 549
PaeR7I CTCGAG 1 cut(s) 115
PasI CCCWGGG 1 cut(s) 159
PcsI WCGNNNNNNNCGW 1 cut(s) 717
PctI GAATGC 1 cut(s) 233
PfeI GAWTC 3 cut(s) 254, 296, 851
PflFI GACNNNGTC 1 cut(s) 644
PkrI GCNGC 3 cut(s) 277, 690, 817
PleI GAGTC 5 cut(s) 20, 98, 172, 382, 406
PpsI GAGTC 5 cut(s) 20, 98, 172, 382, 406
Psp6I CCWGG 5 cut(s) 158, 196, 262, 487, 790
PspFI CCCAGC 1 cut(s) 846
PspGI CCWGG 5 cut(s) 158, 196, 262, 487, 790
PspN4I GGNNCC 1 cut(s) 513
PspPI GGNCC 3 cut(s) 14, 536, 1042
PspXI VCTCGAGB 1 cut(s) 115
PstI CTGCAG 1 cut(s) 561
PsuI RGATCY 1 cut(s) 511
PsyI GACNNNGTC 1 cut(s) 644
RsaI GTAC 4 cut(s) 140, 554, 726, 779
RsaNI GTAC 4 cut(s) 139, 553, 725, 778
RseI CAYNNNNRTG 3 cut(s) 226, 594, 1050
SalI GTCGAC 3 cut(s) 166, 308, 981
SaqAI TTAA 2 cut(s) 204, 821
SatI GCNGC 3 cut(s) 276, 689, 816
Sau3AI GATC 4 cut(s) 491, 511, 712, 1054
Sau96I GGNCC 3 cut(s) 14, 536, 1042
SbfI CCTGCAGG 1 cut(s) 561
SchI GAGTC 5 cut(s) 20, 98, 173, 383, 407
ScrFI CCNGG 5 cut(s) 160, 198, 264, 489, 792
SdaI CCTGCAGG 1 cut(s) 561
SduI GDGCHC 1 cut(s) 439
SfcI CTRYAG 1 cut(s) 557
Sfr274I CTCGAG 1 cut(s) 115
SinI GGWCC 3 cut(s) 14, 536, 1042
SlaI CTCGAG 1 cut(s) 115
SmiMI CAYNNNNRTG 3 cut(s) 226, 594, 1050
SmlI CTYRAG 2 cut(s) 115, 232
SmoI CTYRAG 2 cut(s) 115, 232
Sse8387I CCTGCAGG 1 cut(s) 561
Sse9I AATT 5 cut(s) 248, 945, 1033, 1066, 1078
SsiI CCGC 1 cut(s) 1027
SspI AATATT 1 cut(s) 699
SspMI CTAG 1 cut(s) 593
StyD4I CCNGG 5 cut(s) 158, 196, 262, 487, 790
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 5 cut(s) 416, 567, 621, 685, 707
TaiI ACGT 1 cut(s) 8
TaqI TCGA 8 cut(s) 116, 167, 309, 396, 648, 665, 711, 982
TasI AATT 5 cut(s) 248, 945, 1033, 1066, 1078
TatI WGTACW 1 cut(s) 777
TfiI GAWTC 3 cut(s) 254, 296, 851
Tru1I TTAA 2 cut(s) 204, 821
Tru9I TTAA 2 cut(s) 204, 821
TscAI CASTG 4 cut(s) 421, 690, 866, 1077
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 3 cut(s) 275, 688, 815
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 6 cut(s) 90, 112, 139, 229, 578, 801
TspGWI ACGGA 1 cut(s) 919
TspRI CASTG 4 cut(s) 421, 690, 866, 1077
Tth111I GACNNNGTC 1 cut(s) 644
VpaK11BI GGWCC 3 cut(s) 14, 536, 1042
XapI RAATTY 2 cut(s) 1033, 1078
XbaI TCTAGA 1 cut(s) 592
XhoI CTCGAG 1 cut(s) 115
XmiI GTMKAC 4 cut(s) 167, 180, 309, 982
XspI CTAG 1 cut(s) 593
ZraI GACGTC 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.