MD10G1261100.v1.1

MuDR family transposase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000885
Physical Location & Seq
Forward (+)
35544869 .. 35547513
2645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1261100.v1.1.491

Sequence Viewer

Length: 1314 bp
ATGAAGGTCGACCCAAATGAATATGGAATTATAATGAAAGCTGTATATAGTGGAAAGTCTCATCCGTATCCTGCGGAAGTAGTAGATGATAATGACGTGGCTGCATTTATTTGTGAGAGTGTTGCACGGGACTGGAAAATTCCATTGTGCGTTACTCTTGAATGCAGGGTATTAGCTTCTGGAAGTGGAAGTAATCAACAAGCTCCAATCGGCCTTGCAACTCATACTACAAAATCTCATTCATTAGATCCCCACACTCAACATGAAGAACAAAATTCCTTTGACTTCATTTCCAGTCCAATGCCAATGGACTTCAGTTCAGGGACTCAAGCAAATTCGGATTGTGTTGATGGATTGATCCATGATGATTTCGAAAATGATAAGGAGCATGATAATATAGAAGTTGATGTGGAACAAAACAACCTGCAACATGATGATGTGCCTTGTGACAAGCTGCAACTTTCTGAGGTGCCTCAGCCAAACTTGCAATTTGATGATTTGCCTCATGACAGCAGGCAATTTGATAATATGTATCATGAAAATATGCAATTTTGCGATCATCATCTCAATCCTCCTAAATCTCTACCTCGAGCTCGGTCTTGGCGTAAACTCACAGAAGAAATGGTTAATAGGATGCTGAATTCGGGGTTGAAAGCAGGGGGAGATATGGAACTAGGTTCAGTGTACAAGAGCAAAAAGGATTTACAGAAGATGTTAGCCCTTGCTGCAATTAAAAGAAGTTTTGAGTTCAAAGTTAAGAGGTCCACGAGAAAGAGATTTTCTGTTGAATGTGTGAATGACAACTGCGCGTGGAAAGTCCATGCAACCAAGTCGCCAGACTCTGATTATTTCATGATAACAAAATATGATAGTCGCCACTCTTGTTCATCAGATGCTAAAAATCTCAGTTCGTTAGATGCAAAAAATGGCAGTCACCGACAAGCAAGCAGCTTGCTCATTGGTGAGTTTGTAAAGTCAAAATACAAGGGTGTTTTGTGCCAATGCAAGCCGAAAAATATCATTGAGGATGTGCAGAAAGATCTTGGAGTGAATATAAGTTATTCGACAGCTAAGAGGGTTTTGGATTGTGCATTGAAAGATGTTGGGGTATCATCACCAGAACTTTCTTCTAAACAGGCGTCAAGTGAAAGGTGCAAGAAGGAAAGAACTCCGTCTCAACCAAAAAAAATGTCCCGTGGAGAAGAGCAGTCTACACGGAATTGTGGAAAATGTGGCTCAACTGGTCATTACCGTAAAACCTGCAAGAACCCTATTACTCTTCATCTTAATTCCGAAAGTGAGTTTGCTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut

Protein Analysis

438

Amino Acids

48.99

Weight (kDa)

6.63

Isoelectric Point (pI)

48.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBD_Tnp_Mut PF03108 223 - 286 1.3e-13 MuDR family transposase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 32
Acc36I ACCTGC 2 cut(s) 432, 1268
AccB1I GGYRCC 1 cut(s) 469
AccI GTMKAC 2 cut(s) 9, 1211
AccII CGCG 1 cut(s) 809
AciI CCGC 1 cut(s) 74
AclWI GGATC 2 cut(s) 242, 352
AcsI RAATTY 4 cut(s) 138, 274, 334, 640
AcuI CTGAAG 1 cut(s) 298
AcyI GRCGYC 1 cut(s) 1139
AfaI GTAC 1 cut(s) 686
AgsI TTSAA 5 cut(s) 161, 652, 751, 788, 1096
AjiI CACGTC 1 cut(s) 97
AluBI AGCT 7 cut(s) 41, 176, 203, 454, 593, 951, 1070
AluI AGCT 7 cut(s) 41, 176, 203, 454, 593, 951, 1070
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 2 cut(s) 63, 1179
AlwI GGATC 2 cut(s) 242, 352
AlwNI CAGNNNCTG 1 cut(s) 842
Ama87I CYCGRG 1 cut(s) 588
AoxI GGCC 1 cut(s) 211
ApeKI GCWGC 5 cut(s) 101, 454, 725, 948, 1307
ApoI RAATTY 4 cut(s) 138, 274, 334, 640
ArsI GACNNNNNNTTYG 2 cut(s) 1175, 1207
AspLEI GCGC 1 cut(s) 809
AspS9I GGNCC 1 cut(s) 762
AsuHPI GGTGA 3 cut(s) 926, 974, 1107
AsuII TTCGAA 1 cut(s) 372
AvaI CYCGRG 1 cut(s) 588
AvaII GGWCC 1 cut(s) 762
BanI GGYRCC 1 cut(s) 469
BanII GRGCYC 1 cut(s) 595
BauI CACGAG 1 cut(s) 766
Bbv12I GWGCWC 1 cut(s) 595
BbvCI CCTCAGC 1 cut(s) 474
BbvI GCAGC 5 cut(s) 88, 441, 712, 960, 1294
BccI CCATC 1 cut(s) 344
BcgI CGANNNNNNTGC 2 cut(s) 813, 847
BciVI GTATCC 1 cut(s) 78
BcoDI GTCTC 2 cut(s) 63, 1179
BfaI CTAG 2 cut(s) 674, 1312
BfuAI ACCTGC 2 cut(s) 432, 1268
BfuI GTATCC 1 cut(s) 78
BglII AGATCT 1 cut(s) 1039
BisI GCNGC 5 cut(s) 102, 455, 726, 949, 1308
BlsI GCNGC 5 cut(s) 103, 456, 727, 950, 1309
Bme18I GGWCC 1 cut(s) 762
BmeT110I CYCGRG 1 cut(s) 588
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 762
BmiI GGNNCC 1 cut(s) 471
BmsI GCATC 3 cut(s) 624, 883, 907
Bpu10I CCTNAGC 1 cut(s) 474
Bpu14I TTCGAA 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 312
BsaBI GATNNNNATC 1 cut(s) 561
BsaHI GRCGYC 1 cut(s) 1139
BsaJI CCNNGG 1 cut(s) 1195
Bse1I ACTGG 3 cut(s) 137, 294, 1246
Bse8I GATNNNNATC 1 cut(s) 561
BseDI CCNNGG 1 cut(s) 1195
BseGI GGATG 3 cut(s) 61, 639, 1033
BseJI GATNNNNATC 1 cut(s) 561
BseMII CTCAG 3 cut(s) 456, 488, 919
BseNI ACTGG 3 cut(s) 137, 294, 1246
BseXI GCAGC 5 cut(s) 88, 441, 712, 960, 1294
BsgI GTGCAG 1 cut(s) 1052
Bsh1236I CGCG 1 cut(s) 809
BshFI GGCC 1 cut(s) 213
BshNI GGYRCC 1 cut(s) 469
BsiHKAI GWGCWC 1 cut(s) 595
BsiHKCI CYCGRG 1 cut(s) 588
BslFI GGGAC 3 cut(s) 143, 337, 1177
BsmAI GTCTC 2 cut(s) 63, 1179
BsmBI CGTCTC 1 cut(s) 1179
BsmFI GGGAC 3 cut(s) 143, 337, 1177
BsmI GAATGC 1 cut(s) 167
BsnI GGCC 1 cut(s) 213
BsoBI CYCGRG 1 cut(s) 588
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 595
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 4 cut(s) 247, 357, 556, 1039
BspACI CCGC 1 cut(s) 74
BspANI GGCC 1 cut(s) 213
BspCNI CTCAG 3 cut(s) 457, 487, 918
BspFNI CGCG 1 cut(s) 809
BspHI TCATGA 3 cut(s) 505, 535, 852
BspLI GGNNCC 1 cut(s) 471
BspMI ACCTGC 2 cut(s) 432, 1268
BspPI GGATC 2 cut(s) 242, 352
BspQI GCTCTTC 1 cut(s) 1197
BspT104I TTCGAA 1 cut(s) 372
BspT107I GGYRCC 1 cut(s) 469
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 3 cut(s) 137, 294, 1246
BssECI CCNNGG 1 cut(s) 1195
BssMI GATC 4 cut(s) 247, 357, 556, 1039
BssNI GRCGYC 1 cut(s) 1139
BssSI CACGAG 1 cut(s) 766
Bst2BI CACGAG 1 cut(s) 766
Bst4CI ACNGT 1 cut(s) 1253
Bst6I CTCTTC 2 cut(s) 1197, 1284
BstACI GRCGYC 1 cut(s) 1139
BstAUI TGTACA 1 cut(s) 684
BstBI TTCGAA 1 cut(s) 372
BstC8I GCNNGC 4 cut(s) 515, 946, 953, 1007
BstDEI CTNAG 4 cut(s) 465, 474, 905, 1071
BstDSI CCRYGG 1 cut(s) 1195
BstF5I GGATG 3 cut(s) 61, 639, 1033
BstFNI CGCG 1 cut(s) 809
BstHHI GCGC 1 cut(s) 809
BstKTI GATC 4 cut(s) 250, 360, 559, 1042
BstMAI GTCTC 2 cut(s) 63, 1179
BstMBI GATC 4 cut(s) 247, 357, 556, 1039
BstMWI GCNNNNNNNGC 2 cut(s) 484, 725
BstUI CGCG 1 cut(s) 809
BstV1I GCAGC 5 cut(s) 88, 441, 712, 960, 1294
BstX2I RGATCY 2 cut(s) 247, 1039
BstYI RGATCY 2 cut(s) 247, 1039
BsuI GTATCC 1 cut(s) 78
BsuRI GGCC 1 cut(s) 213
BtgI CCRYGG 1 cut(s) 1195
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 3 cut(s) 61, 639, 1033
BtsIMutI CAGTG 1 cut(s) 687
BveI ACCTGC 2 cut(s) 432, 1268
Cac8I GCNNGC 4 cut(s) 515, 946, 953, 1007
CaiI CAGNNNCTG 1 cut(s) 842
CciI TCATGA 3 cut(s) 505, 535, 852
CfoI GCGC 1 cut(s) 809
Cfr13I GGNCC 1 cut(s) 762
CseI GACGC 1 cut(s) 1128
Csp6I GTAC 1 cut(s) 685
CviAII CATG 8 cut(s) 263, 362, 389, 431, 506, 536, 821, 853
CviQI GTAC 1 cut(s) 685
DdeI CTNAG 4 cut(s) 465, 474, 905, 1071
DpnI GATC 4 cut(s) 249, 359, 558, 1041
DpnII GATC 4 cut(s) 247, 357, 556, 1039
Eam1104I CTCTTC 2 cut(s) 1197, 1284
EarI CTCTTC 2 cut(s) 1197, 1284
Ecl136II GAGCTC 1 cut(s) 593
Eco24I GRGCYC 1 cut(s) 595
Eco47I GGWCC 1 cut(s) 762
Eco53kI GAGCTC 1 cut(s) 593
Eco57I CTGAAG 1 cut(s) 298
Eco88I CYCGRG 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 593
EcoRI GAATTC 1 cut(s) 640
EcoT38I GRGCYC 1 cut(s) 595
Esp3I CGTCTC 1 cut(s) 1179
FaeI CATG 8 cut(s) 266, 365, 392, 434, 509, 539, 824, 856
FaqI GGGAC 3 cut(s) 143, 337, 1177
FatI CATG 8 cut(s) 262, 361, 388, 430, 505, 535, 820, 852
FblI GTMKAC 2 cut(s) 9, 1211
Fnu4HI GCNGC 5 cut(s) 102, 455, 726, 949, 1308
FokI GGATG 3 cut(s) 48, 646, 1040
FriOI GRGCYC 1 cut(s) 595
Fsp4HI GCNGC 5 cut(s) 102, 455, 726, 949, 1308
FspBI CTAG 2 cut(s) 674, 1312
GlaI GCGC 1 cut(s) 808
GluI GCNGC 5 cut(s) 102, 455, 726, 949, 1308
HaeIII GGCC 1 cut(s) 213
HgaI GACGC 1 cut(s) 1128
HhaI GCGC 1 cut(s) 809
Hin1I GRCGYC 1 cut(s) 1139
Hin1II CATG 8 cut(s) 266, 365, 392, 434, 509, 539, 824, 856
Hin6I GCGC 1 cut(s) 807
HinP1I GCGC 1 cut(s) 807
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HinfI GANTC 2 cut(s) 325, 839
HphI GGTGA 3 cut(s) 926, 974, 1107
Hpy166II GTNNAC 5 cut(s) 10, 608, 685, 765, 1212
Hpy188I TCNGA 5 cut(s) 340, 466, 844, 892, 1294
Hpy188III TCNNGA 5 cut(s) 158, 180, 506, 536, 853
Hpy8I GTNNAC 5 cut(s) 10, 608, 685, 765, 1212
HpyAV CCTTC 1 cut(s) 1153
HpyCH4III ACNGT 1 cut(s) 1253
HpyCH4IV ACGT 1 cut(s) 96
HpyF10VI GCNNNNNNNGC 2 cut(s) 484, 725
HpyF3I CTNAG 4 cut(s) 465, 474, 905, 1071
HpySE526I ACGT 1 cut(s) 96
Hsp92I GRCGYC 1 cut(s) 1139
Hsp92II CATG 8 cut(s) 266, 365, 392, 434, 509, 539, 824, 856
HspAI GCGC 1 cut(s) 807
Kzo9I GATC 4 cut(s) 247, 357, 556, 1039
LguI GCTCTTC 1 cut(s) 1197
LmnI GCTCC 2 cut(s) 208, 385
Lsp1109I GCAGC 5 cut(s) 88, 441, 712, 960, 1294
LweI GCATC 3 cut(s) 624, 883, 907
MaeI CTAG 2 cut(s) 674, 1312
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 3 cut(s) 151, 446, 932
MalI GATC 4 cut(s) 249, 359, 558, 1041
MboI GATC 4 cut(s) 247, 357, 556, 1039
MboII GAAGA 6 cut(s) 278, 629, 721, 1119, 1214, 1271
MflI RGATCY 2 cut(s) 247, 1039
MhlI GDGCHC 1 cut(s) 595
MlyI GAGTC 2 cut(s) 319, 833
MnlI CCTC 8 cut(s) 460, 483, 513, 582, 597, 753, 1018, 1068
MseI TTAA 4 cut(s) 627, 732, 756, 1287
MslI CAYNNNNRTG 1 cut(s) 435
Mva1269I GAATGC 1 cut(s) 167
MvnI CGCG 1 cut(s) 809
MwoI GCNNNNNNNGC 2 cut(s) 484, 725
NdeII GATC 4 cut(s) 247, 357, 556, 1039
NlaIII CATG 8 cut(s) 266, 365, 392, 434, 509, 539, 824, 856
NlaIV GGNNCC 1 cut(s) 471
NmuCI GTSAC 2 cut(s) 446, 932
NspV TTCGAA 1 cut(s) 372
PaeR7I CTCGAG 1 cut(s) 588
PagI TCATGA 3 cut(s) 505, 535, 852
PciSI GCTCTTC 1 cut(s) 1197
PcsI WCGNNNNNNNCGW 1 cut(s) 601
PctI GAATGC 1 cut(s) 167
PkrI GCNGC 5 cut(s) 103, 456, 727, 950, 1309
PleI GAGTC 2 cut(s) 319, 833
PpsI GAGTC 2 cut(s) 319, 833
PsiI TTATAA 1 cut(s) 32
Psp124BI GAGCTC 1 cut(s) 595
PspN4I GGNNCC 1 cut(s) 471
PspPI GGNCC 1 cut(s) 762
PspXI VCTCGAGB 1 cut(s) 588
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 2 cut(s) 247, 1039
RsaI GTAC 1 cut(s) 686
RsaNI GTAC 1 cut(s) 685
RseI CAYNNNNRTG 1 cut(s) 435
SacI GAGCTC 1 cut(s) 595
SalI GTCGAC 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 1197
SaqAI TTAA 4 cut(s) 627, 732, 756, 1287
SatI GCNGC 5 cut(s) 102, 455, 726, 949, 1308
Sau3AI GATC 4 cut(s) 247, 357, 556, 1039
Sau96I GGNCC 1 cut(s) 762
SchI GAGTC 2 cut(s) 319, 833
SduI GDGCHC 1 cut(s) 595
SfaNI GCATC 3 cut(s) 624, 883, 907
Sfr274I CTCGAG 1 cut(s) 588
SfuI TTCGAA 1 cut(s) 372
SinI GGWCC 1 cut(s) 762
SlaI CTCGAG 1 cut(s) 588
SmiMI CAYNNNNRTG 1 cut(s) 435
SmlI CTYRAG 2 cut(s) 327, 588
SmoI CTYRAG 2 cut(s) 327, 588
SsiI CCGC 1 cut(s) 74
SspMI CTAG 2 cut(s) 674, 1312
SstI GAGCTC 1 cut(s) 595
TaaI ACNGT 1 cut(s) 1253
TaiI ACGT 1 cut(s) 99
TaqI TCGA 4 cut(s) 9, 372, 589, 1064
TaqII GACCGA 1 cut(s) 585
TatI WGTACW 1 cut(s) 684
Tru1I TTAA 4 cut(s) 627, 732, 756, 1287
Tru9I TTAA 4 cut(s) 627, 732, 756, 1287
TscAI CASTG 1 cut(s) 687
TseFI GTSAC 2 cut(s) 446, 932
TseI GCWGC 5 cut(s) 101, 454, 725, 948, 1307
Tsp45I GTSAC 2 cut(s) 446, 932
TspGWI ACGGA 3 cut(s) 54, 1161, 1231
TspRI CASTG 1 cut(s) 687
VpaK11BI GGWCC 1 cut(s) 762
XapI RAATTY 4 cut(s) 138, 274, 334, 640
XhoI CTCGAG 1 cut(s) 588
XmiI GTMKAC 2 cut(s) 9, 1211
XspI CTAG 2 cut(s) 674, 1312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.