RLG00000033652

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000885
Physical Location & Seq
Reverse (-)
30202277 .. 30204430
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033652

Sequence Viewer

Length: 1176 bp
ATGCCGTCCGATCATCGATGGACCGAACTCCATGCCTTACTAGACGAAGTGGACGAAATCCAGTTTCTGCTATCCCGGCCGACTGAACTTTCAGTGACGTCCAATGATCTATCCACAACGCTCCGTGCTGTAAGGGACCGAGTGAATAAAATCCGGAATTCCTACCACGATGAATTGGAGGCCGTCCTGGACTGCAAACTCGTTTTAACATCTCTAGAGGGTCTCGTTCTGGCTCTTGACTTAGCTGAGAAAATTAATTGCATTTCCGAAGGAGAACGTCCACAGATCGGGTTACGCCTGGCACTACTGAATAATATAATTGGATCCTATTCGAAAAGTATATATATTATCACCGGAAGTTTGGCACGGTCGGAGAGGGAATTGGATGACCTCTCGGAGGTTCTCGGAGGCTCGAATAATAATAATGTAGGAAAAACAGTGCTGCCAATATTCTACGGTGTTGATCCCTCCGATGTACGACATCAAACAGGAAGTTTTGCAGAAGCCTTTGCAGACCATGAGAAGAAGTACAAAGATGACCCACAAAAAGTAAAAAGGTGGAGAGCTGCTTTAACGAAACTGGCGAATCTCGCTGGCTGGGATTCAAAGCACTGGCCCGAAACAAAACTCATTCGAGAAGTTGTTGATGAGATATGGATGAAATTGTATTCTTCTTTTTCAAAACCTGCCATTACAAATGTGCCATCAACACCTTCTTGGTCCTCTTCTTGTCGACAGAGGCATGATGTTTTTCTAAGCTTTAGAGGGAAGGATATCCGCTTGAATTTTTTGGACCATCTATATGATGCATTAATTCAGCAAAAAATTTCCACCTTTAGAGATGACAAAGATCTTCAGAAAGGAATGTCCATTCGGAAACAACTCGACAATGCAATTGAGGGATCAAAGGTTTGGGTTGTCATTGTTTCACCAGAGTATGCTTCTTCAAGATGGTGCTTGGATGAGCTTTTGAAGATTCTTGAATGTAGAAAAGTATCTAGAGTCGAAAATAAGAATCAAACTTTGATGAAGGCAGTTGCAGAACATGAAGAACAAGCTCATACTTTGGTGAATGGAGACAAAACACACAACGGGAAGGCTAGTATTCAGAACAAGGAAACCATCAGTAGTTATAATAAAAGGTTATTCATCTTCAGTTTCATGCATGTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

392

Amino Acids

44.61

Weight (kDa)

6.65

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 105 - 228 5.7e-22 TIR domain
TIR PF01582 248 - 333 5.8e-28 TIR domain
TIR_2 PF13676 250 - 328 1.2e-14 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1134
AatII GACGTC 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 694
AccI GTMKAC 1 cut(s) 733
AccIII TCCGGA 1 cut(s) 153
AciI CCGC 1 cut(s) 778
AclWI GGATC 4 cut(s) 318, 331, 458, 910
AcoI YGGCCR 1 cut(s) 77
AcsI RAATTY 3 cut(s) 157, 784, 825
AcuI CTGAAG 2 cut(s) 839, 1138
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 2 cut(s) 477, 530
AfiI CCNNNNNNNGG 2 cut(s) 287, 397
AgsI TTSAA 6 cut(s) 606, 681, 784, 948, 973, 983
AjnI CCWGG 2 cut(s) 186, 297
AluBI AGCT 5 cut(s) 245, 566, 759, 967, 1058
AluI AGCT 5 cut(s) 245, 566, 759, 967, 1058
Alw26I GTCTC 2 cut(s) 227, 1071
AlwI GGATC 4 cut(s) 318, 331, 458, 910
AlwNI CAGNNNCTG 1 cut(s) 67
Aor13HI TCCGGA 1 cut(s) 153
AoxI GGCC 3 cut(s) 77, 180, 614
ApeKI GCWGC 2 cut(s) 442, 566
ApoI RAATTY 3 cut(s) 157, 784, 825
AseI ATTAAT 2 cut(s) 255, 812
AspS9I GGNCC 5 cut(s) 21, 136, 615, 720, 793
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 3 cut(s) 343, 921, 1081
AsuII TTCGAA 1 cut(s) 332
AvaII GGWCC 4 cut(s) 21, 136, 720, 793
BamHI GGATCC 1 cut(s) 323
BbvI GCAGC 2 cut(s) 429, 553
BccI CCATC 5 cut(s) 12, 712, 804, 945, 1130
BceAI ACGGC 1 cut(s) 167
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 2 cut(s) 188, 299
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 2 cut(s) 227, 1071
BfaI CTAG 4 cut(s) 41, 215, 999, 1101
BfuAI ACCTGC 1 cut(s) 694
BglII AGATCT 1 cut(s) 850
BisI GCNGC 2 cut(s) 443, 567
BlsI GCNGC 2 cut(s) 444, 568
Bme1390I CCNGG 3 cut(s) 76, 188, 299
Bme18I GGWCC 4 cut(s) 21, 136, 720, 793
BmgT120I GGNCC 5 cut(s) 21, 136, 615, 720, 793
BmiI GGNNCC 2 cut(s) 137, 325
BmrFI CCNGG 3 cut(s) 76, 188, 299
BmsI GCATC 1 cut(s) 796
Bpu14I TTCGAA 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 76
Bsa29I ATCGAT 1 cut(s) 16
BsaHI GRCGYC 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 227
BsaWI WCCGGW 2 cut(s) 153, 353
Bsc4I CCNNNNNNNGG 2 cut(s) 287, 397
Bse1I ACTGG 3 cut(s) 61, 585, 617
BseAI TCCGGA 1 cut(s) 153
BseBI CCWGG 2 cut(s) 188, 299
BseCI ATCGAT 1 cut(s) 16
BseGI GGATG 3 cut(s) 391, 663, 967
BseLI CCNNNNNNNGG 2 cut(s) 287, 397
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 3 cut(s) 61, 585, 617
BseX3I CGGCCG 1 cut(s) 77
BseXI GCAGC 2 cut(s) 429, 553
BseYI CCCAGC 1 cut(s) 597
Bsh1285I CGRYCG 2 cut(s) 80, 371
BshFI GGCC 3 cut(s) 79, 182, 616
BshVI ATCGAT 1 cut(s) 16
BsiEI CGRYCG 2 cut(s) 80, 371
BsiSI CCGG 3 cut(s) 76, 154, 354
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 2 cut(s) 287, 397
BsmAI GTCTC 2 cut(s) 227, 1071
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 3 cut(s) 79, 182, 616
Bso31I GGTCTC 1 cut(s) 227
Bsp119I TTCGAA 1 cut(s) 332
Bsp13I TCCGGA 1 cut(s) 153
Bsp143I GATC 7 cut(s) 10, 106, 285, 323, 463, 850, 902
BspACI CCGC 1 cut(s) 778
BspANI GGCC 3 cut(s) 79, 182, 616
BspCNI CTCAG 1 cut(s) 238
BspDI ATCGAT 1 cut(s) 16
BspEI TCCGGA 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 137, 325
BspMI ACCTGC 1 cut(s) 694
BspPI GGATC 4 cut(s) 318, 331, 458, 910
BspT104I TTCGAA 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 227
BsrI ACTGG 3 cut(s) 61, 585, 617
BssMI GATC 7 cut(s) 10, 106, 285, 323, 463, 850, 902
BssNI GRCGYC 1 cut(s) 98
Bst2UI CCWGG 2 cut(s) 188, 299
Bst4CI ACNGT 3 cut(s) 369, 439, 458
Bst6I CTCTTC 1 cut(s) 730
BstACI GRCGYC 1 cut(s) 98
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 595
BstDEI CTNAG 3 cut(s) 241, 246, 755
BstENI CCTNNNNNAGG 1 cut(s) 395
BstF5I GGATG 3 cut(s) 391, 663, 967
BstKTI GATC 7 cut(s) 13, 109, 288, 326, 466, 853, 905
BstMAI GTCTC 2 cut(s) 227, 1071
BstMBI GATC 7 cut(s) 10, 106, 285, 323, 463, 850, 902
BstMCI CGRYCG 2 cut(s) 80, 371
BstMWI GCNNNNNNNGC 2 cut(s) 76, 590
BstNI CCWGG 2 cut(s) 188, 299
BstNSI RCATGY 1 cut(s) 1169
BstSCI CCNGG 3 cut(s) 74, 186, 297
BstV1I GCAGC 2 cut(s) 429, 553
BstX2I RGATCY 2 cut(s) 323, 850
BstYI RGATCY 2 cut(s) 323, 850
BstZI CGGCCG 1 cut(s) 77
Bsu15I ATCGAT 1 cut(s) 16
BsuRI GGCC 3 cut(s) 79, 182, 616
BsuTUI ATCGAT 1 cut(s) 16
BtsCI GGATG 3 cut(s) 391, 663, 967
BtsIMutI CAGTG 3 cut(s) 99, 444, 610
BveI ACCTGC 1 cut(s) 694
Cac8I GCNNGC 1 cut(s) 595
CaiI CAGNNNCTG 1 cut(s) 67
Cfr13I GGNCC 5 cut(s) 21, 136, 615, 720, 793
ClaI ATCGAT 1 cut(s) 16
Csp6I GTAC 2 cut(s) 476, 529
CviAII CATG 6 cut(s) 32, 518, 743, 1046, 1162, 1166
CviQI GTAC 2 cut(s) 476, 529
DdeI CTNAG 3 cut(s) 241, 246, 755
DpnI GATC 7 cut(s) 12, 108, 287, 325, 465, 852, 904
DpnII GATC 7 cut(s) 10, 106, 285, 323, 463, 850, 902
EaeI YGGCCR 1 cut(s) 77
EagI CGGCCG 1 cut(s) 77
Eam1104I CTCTTC 1 cut(s) 730
EarI CTCTTC 1 cut(s) 730
EclXI CGGCCG 1 cut(s) 77
Eco31I GGTCTC 1 cut(s) 227
Eco32I GATATC 1 cut(s) 775
Eco47I GGWCC 4 cut(s) 21, 136, 720, 793
Eco52I CGGCCG 1 cut(s) 77
Eco57I CTGAAG 2 cut(s) 839, 1138
EcoNI CCTNNNNNAGG 1 cut(s) 395
EcoRI GAATTC 1 cut(s) 157
EcoRII CCWGG 2 cut(s) 186, 297
EcoRV GATATC 1 cut(s) 775
EcoT22I ATGCAT 2 cut(s) 811, 1167
FaeI CATG 6 cut(s) 35, 521, 746, 1049, 1165, 1169
FaqI GGGAC 1 cut(s) 149
FatI CATG 6 cut(s) 31, 517, 742, 1045, 1161, 1165
FblI GTMKAC 1 cut(s) 733
Fnu4HI GCNGC 2 cut(s) 443, 567
FokI GGATG 3 cut(s) 398, 670, 974
Fsp4HI GCNGC 2 cut(s) 443, 567
FspBI CTAG 4 cut(s) 41, 215, 999, 1101
GluI GCNGC 2 cut(s) 443, 567
GsaI CCCAGC 1 cut(s) 601
HaeIII GGCC 3 cut(s) 79, 182, 616
HapII CCGG 3 cut(s) 76, 154, 354
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 6 cut(s) 35, 521, 746, 1049, 1165, 1169
HincII GTYRAC 1 cut(s) 734
HindII GTYRAC 1 cut(s) 734
HindIII AAGCTT 1 cut(s) 757
HinfI GANTC 5 cut(s) 586, 602, 976, 1002, 1015
HpaII CCGG 3 cut(s) 76, 154, 354
HphI GGTGA 3 cut(s) 343, 921, 1081
Hpy166II GTNNAC 3 cut(s) 52, 281, 734
Hpy188I TCNGA 9 cut(s) 10, 268, 373, 397, 407, 472, 858, 876, 1110
Hpy188III TCNNGA 7 cut(s) 154, 215, 236, 635, 948, 980, 999
Hpy8I GTNNAC 3 cut(s) 52, 281, 734
HpyAV CCTTC 5 cut(s) 263, 723, 763, 1024, 1090
HpyCH4III ACNGT 3 cut(s) 369, 439, 458
HpyCH4IV ACGT 2 cut(s) 98, 277
HpyCH4V TGCA 8 cut(s) 195, 261, 500, 512, 809, 893, 1040, 1165
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 590
HpyF3I CTNAG 3 cut(s) 241, 246, 755
HpySE526I ACGT 2 cut(s) 98, 277
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 6 cut(s) 35, 521, 746, 1049, 1165, 1169
Kpn2I TCCGGA 1 cut(s) 153
Kzo9I GATC 7 cut(s) 10, 106, 285, 323, 463, 850, 902
LmnI GCTCC 1 cut(s) 126
Lsp1109I GCAGC 2 cut(s) 429, 553
LweI GCATC 1 cut(s) 796
MaeI CTAG 4 cut(s) 41, 215, 999, 1101
MaeII ACGT 2 cut(s) 98, 277
MaeIII GTNAC 2 cut(s) 94, 291
MalI GATC 7 cut(s) 12, 108, 287, 325, 465, 852, 904
MboI GATC 7 cut(s) 10, 106, 285, 323, 463, 850, 902
MboII GAAGA 8 cut(s) 535, 663, 717, 845, 936, 985, 1061, 1144
MfeI CAATTG 1 cut(s) 894
MflI RGATCY 2 cut(s) 323, 850
MlyI GAGTC 1 cut(s) 1011
MmeI TCCRAC 1 cut(s) 351
Mph1103I ATGCAT 2 cut(s) 811, 1167
MroI TCCGGA 1 cut(s) 153
MseI TTAA 5 cut(s) 206, 255, 572, 812, 1174
MslI CAYNNNNRTG 1 cut(s) 801
MspI CCGG 3 cut(s) 76, 154, 354
MspR9I CCNGG 3 cut(s) 76, 188, 299
MunI CAATTG 1 cut(s) 894
MvaI CCWGG 2 cut(s) 188, 299
MwoI GCNNNNNNNGC 2 cut(s) 76, 590
NciI CCSGG 1 cut(s) 76
NdeII GATC 7 cut(s) 10, 106, 285, 323, 463, 850, 902
NlaIII CATG 6 cut(s) 35, 521, 746, 1049, 1165, 1169
NlaIV GGNNCC 2 cut(s) 137, 325
NmuCI GTSAC 1 cut(s) 94
NsiI ATGCAT 2 cut(s) 811, 1167
NspI RCATGY 1 cut(s) 1169
NspV TTCGAA 1 cut(s) 332
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 581
PfeI GAWTC 4 cut(s) 586, 602, 976, 1015
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 2 cut(s) 444, 568
PleI GAGTC 1 cut(s) 1010
PpsI GAGTC 1 cut(s) 1010
PshBI ATTAAT 2 cut(s) 255, 812
PsiI TTATAA 1 cut(s) 1134
Psp6I CCWGG 2 cut(s) 186, 297
PspFI CCCAGC 1 cut(s) 597
PspGI CCWGG 2 cut(s) 186, 297
PspN4I GGNNCC 2 cut(s) 137, 325
PspPI GGNCC 5 cut(s) 21, 136, 615, 720, 793
PstNI CAGNNNCTG 1 cut(s) 67
PsuI RGATCY 2 cut(s) 323, 850
RsaI GTAC 2 cut(s) 477, 530
RsaNI GTAC 2 cut(s) 476, 529
RseI CAYNNNNRTG 1 cut(s) 801
SalI GTCGAC 1 cut(s) 732
SaqAI TTAA 5 cut(s) 206, 255, 572, 812, 1174
SatI GCNGC 2 cut(s) 443, 567
Sau3AI GATC 7 cut(s) 10, 106, 285, 323, 463, 850, 902
Sau96I GGNCC 5 cut(s) 21, 136, 615, 720, 793
SchI GAGTC 1 cut(s) 1011
ScrFI CCNGG 3 cut(s) 76, 188, 299
SfaNI GCATC 1 cut(s) 796
SfuI TTCGAA 1 cut(s) 332
SinI GGWCC 4 cut(s) 21, 136, 720, 793
SmiMI CAYNNNNRTG 1 cut(s) 801
SsiI CCGC 1 cut(s) 778
SspI AATATT 1 cut(s) 450
SspMI CTAG 4 cut(s) 41, 215, 999, 1101
StyD4I CCNGG 3 cut(s) 74, 186, 297
TaaI ACNGT 3 cut(s) 369, 439, 458
TaiI ACGT 2 cut(s) 101, 280
TaqI TCGA 7 cut(s) 16, 332, 413, 634, 733, 885, 1005
TaqII GACCGA 2 cut(s) 38, 153
TatI WGTACW 1 cut(s) 528
TfiI GAWTC 4 cut(s) 586, 602, 976, 1015
Tru1I TTAA 5 cut(s) 206, 255, 572, 812, 1174
Tru9I TTAA 5 cut(s) 206, 255, 572, 812, 1174
TscAI CASTG 3 cut(s) 99, 444, 617
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 2 cut(s) 442, 566
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 6 cut(s) 186, 674, 1043, 1062, 1138, 1150
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 3 cut(s) 99, 444, 617
VpaK11BI GGWCC 4 cut(s) 21, 136, 720, 793
VspI ATTAAT 2 cut(s) 255, 812
XagI CCTNNNNNAGG 1 cut(s) 395
XapI RAATTY 3 cut(s) 157, 784, 825
XbaI TCTAGA 2 cut(s) 214, 998
XceI RCATGY 1 cut(s) 1169
XmiI GTMKAC 1 cut(s) 733
XspI CTAG 4 cut(s) 41, 215, 999, 1101
ZraI GACGTC 1 cut(s) 99
Zsp2I ATGCAT 2 cut(s) 811, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.