RLG00000005586
NBS-LRR Family

Disease resistance protein (TIR-NBS-LRR class)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000885
Physical Location & Seq
Forward (+)
65001 .. 66244
1244 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005586

Sequence Viewer

Length: 516 bp
ATGATCCAGTTAGAAATCTGGAATAGTGGGTTTCATACCTTGGTTTTGGATAAGGAATGTCGAAGAGTGCCCGAGAGGGGGGGGGACATCCCATATTACGACCCATGGGTCATCTTCAGGGACGATCCATGGGACGTAGTGTGTAAATCGCTGAATCGCGGAAGGGAATTTCGGGGAAGGGCACGGCAACGAGAAAGAGCATTTTCACTTTCCGAGCTTCATCGAGATATTCTATATGAACTCCTTCTGAGTGTTGAGATGTCTAAGAATAGGCGTAGGTATCCACGTCTCCAGTTCAACCGGGGACGATTGAAAATGTTAGAAAAGATGTATGGAACCTGTTCGGACGCTCGTATATATGTTGTCACTCAAAGTTTGGCACGGTCGGAGAGGGCATTGGATGACCTCTCGGAGCTTCTTGAAGCCGCGAAGAATGACATAGAAAAAACAGTGCTGCCAATATTCTACGGTGTTGATCCCTCCGATGTACGACATCAAACAGGAAGTTTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

172

Amino Acids

20.17

Weight (kDa)

8.8

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 116 - 170 1.4e-09 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AccII CGCG 2 cut(s) 159, 428
AciI CCGC 2 cut(s) 159, 426
AclWI GGATC 2 cut(s) 119, 470
AcsI RAATTY 1 cut(s) 167
AcuI CTGAAG 1 cut(s) 100
AfaI GTAC 1 cut(s) 489
AfiI CCNNNNNNNGG 2 cut(s) 77, 78
AgsI TTSAA 3 cut(s) 298, 313, 422
AjiI CACGTC 1 cut(s) 287
AluBI AGCT 2 cut(s) 217, 415
AluI AGCT 2 cut(s) 217, 415
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 2 cut(s) 119, 470
Ama87I CYCGRG 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 454
ApoI RAATTY 1 cut(s) 167
Asp700I GAANNNNTTC 2 cut(s) 243, 340
AsuC2I CCSGG 1 cut(s) 302
AvaI CYCGRG 1 cut(s) 71
BaeGI GKGCMC 2 cut(s) 72, 184
BbvI GCAGC 1 cut(s) 441
BceAI ACGGC 1 cut(s) 200
BciVI GTATCC 1 cut(s) 291
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 293
BfuI GTATCC 1 cut(s) 291
BisI GCNGC 2 cut(s) 426, 455
BlsI GCNGC 2 cut(s) 427, 456
Bme1390I CCNGG 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 71
BmgBI CACGTC 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 337
BmrFI CCNGG 1 cut(s) 302
BpmI CTGGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 302
BsaJI CCNNGG 4 cut(s) 39, 104, 128, 301
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 78
Bse1I ACTGG 2 cut(s) 7, 292
BseDI CCNNGG 4 cut(s) 39, 104, 128, 301
BseGI GGATG 2 cut(s) 87, 406
BseLI CCNNNNNNNGG 2 cut(s) 77, 78
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 2 cut(s) 7, 292
BseSI GKGCMC 2 cut(s) 72, 184
BseXI GCAGC 1 cut(s) 441
Bsh1236I CGCG 2 cut(s) 159, 428
Bsh1285I CGRYCG 1 cut(s) 386
BsiEI CGRYCG 1 cut(s) 386
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 1 cut(s) 301
BslFI GGGAC 4 cut(s) 98, 134, 146, 318
BslI CCNNNNNNNGG 2 cut(s) 77, 78
BsmAI GTCTC 1 cut(s) 293
BsmBI CGTCTC 1 cut(s) 293
BsmFI GGGAC 4 cut(s) 98, 134, 146, 318
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 72, 184
Bsp143I GATC 3 cut(s) 3, 124, 475
Bsp19I CCATGG 2 cut(s) 104, 128
BspACI CCGC 2 cut(s) 159, 426
BspCNI CTCAG 1 cut(s) 240
BspFNI CGCG 2 cut(s) 159, 428
BspLI GGNNCC 1 cut(s) 337
BspPI GGATC 2 cut(s) 119, 470
BsrI ACTGG 2 cut(s) 7, 292
BssECI CCNNGG 4 cut(s) 39, 104, 128, 301
BssMI GATC 3 cut(s) 3, 124, 475
BssT1I CCWWGG 3 cut(s) 39, 104, 128
Bst4CI ACNGT 3 cut(s) 384, 451, 470
Bst6I CTCTTC 1 cut(s) 58
BstDEI CTNAG 2 cut(s) 248, 264
BstDSI CCRYGG 2 cut(s) 104, 128
BstF5I GGATG 2 cut(s) 87, 406
BstFNI CGCG 2 cut(s) 159, 428
BstKTI GATC 3 cut(s) 6, 127, 478
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 3 cut(s) 3, 124, 475
BstMCI CGRYCG 1 cut(s) 386
BstSCI CCNGG 1 cut(s) 300
BstSLI GKGCMC 2 cut(s) 72, 184
BstUI CGCG 2 cut(s) 159, 428
BstV1I GCAGC 1 cut(s) 441
BsuI GTATCC 1 cut(s) 291
BtgI CCRYGG 2 cut(s) 104, 128
BtrI CACGTC 1 cut(s) 287
BtsCI GGATG 2 cut(s) 87, 406
BtsIMutI CAGTG 1 cut(s) 456
CseI GACGC 1 cut(s) 356
Csp6I GTAC 1 cut(s) 488
CviAII CATG 2 cut(s) 105, 129
CviJI RGCY 3 cut(s) 217, 415, 425
CviKI_1 RGCY 3 cut(s) 217, 415, 425
CviQI GTAC 1 cut(s) 488
DdeI CTNAG 2 cut(s) 248, 264
DpnI GATC 3 cut(s) 5, 126, 477
DpnII GATC 3 cut(s) 3, 124, 475
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
Eam1104I CTCTTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 58
Eco130I CCWWGG 3 cut(s) 39, 104, 128
Eco57I CTGAAG 1 cut(s) 100
Eco88I CYCGRG 1 cut(s) 71
EcoT14I CCWWGG 3 cut(s) 39, 104, 128
ErhI CCWWGG 3 cut(s) 39, 104, 128
Esp3I CGTCTC 1 cut(s) 293
FaeI CATG 2 cut(s) 108, 132
FaqI GGGAC 4 cut(s) 98, 134, 146, 318
FatI CATG 2 cut(s) 104, 128
Fnu4HI GCNGC 2 cut(s) 426, 455
FokI GGATG 2 cut(s) 74, 413
Fsp4HI GCNGC 2 cut(s) 426, 455
GluI GCNGC 2 cut(s) 426, 455
GsuI CTGGAG 1 cut(s) 275
HapII CCGG 1 cut(s) 301
HgaI GACGC 1 cut(s) 356
Hin1II CATG 2 cut(s) 108, 132
HinfI GANTC 1 cut(s) 154
HpaII CCGG 1 cut(s) 301
Hpy188I TCNGA 6 cut(s) 214, 249, 346, 388, 412, 484
Hpy188III TCNNGA 3 cut(s) 19, 224, 419
HpyAV CCTTC 3 cut(s) 156, 171, 254
HpyCH4III ACNGT 3 cut(s) 384, 451, 470
HpyCH4IV ACGT 2 cut(s) 135, 286
HpyCH4V TGCA 1 cut(s) 512
HpyF3I CTNAG 2 cut(s) 248, 264
HpySE526I ACGT 2 cut(s) 135, 286
Hsp92II CATG 2 cut(s) 108, 132
Kzo9I GATC 3 cut(s) 3, 124, 475
LmnI GCTCC 1 cut(s) 412
LpnPI CCDG 7 cut(s) 4, 20, 103, 305, 314, 352, 486
Lsp1109I GCAGC 1 cut(s) 441
MaeII ACGT 2 cut(s) 135, 286
MaeIII GTNAC 1 cut(s) 364
MalI GATC 3 cut(s) 5, 126, 477
MboI GATC 3 cut(s) 3, 124, 475
MboII GAAGA 3 cut(s) 75, 106, 442
MhlI GDGCHC 2 cut(s) 72, 184
MluCI AATT 1 cut(s) 167
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 4 cut(s) 69, 384, 416, 490
MroXI GAANNNNTTC 2 cut(s) 243, 340
MspI CCGG 1 cut(s) 301
MspR9I CCNGG 1 cut(s) 302
MvnI CGCG 2 cut(s) 159, 428
NciI CCSGG 1 cut(s) 302
NcoI CCATGG 2 cut(s) 104, 128
NdeII GATC 3 cut(s) 3, 124, 475
NlaIII CATG 2 cut(s) 108, 132
NlaIV GGNNCC 1 cut(s) 337
NmuCI GTSAC 1 cut(s) 364
PdmI GAANNNNTTC 2 cut(s) 243, 340
PfeI GAWTC 1 cut(s) 154
PkrI GCNGC 2 cut(s) 427, 456
PspN4I GGNNCC 1 cut(s) 337
RsaI GTAC 1 cut(s) 489
RsaNI GTAC 1 cut(s) 488
SatI GCNGC 2 cut(s) 426, 455
Sau3AI GATC 3 cut(s) 3, 124, 475
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 2 cut(s) 72, 184
SetI ASST 8 cut(s) 41, 138, 219, 281, 289, 341, 408, 417
Sse9I AATT 1 cut(s) 167
SsiI CCGC 2 cut(s) 159, 426
SspI AATATT 1 cut(s) 462
StyD4I CCNGG 1 cut(s) 300
StyI CCWWGG 3 cut(s) 39, 104, 128
TaaI ACNGT 3 cut(s) 384, 451, 470
TaiI ACGT 2 cut(s) 138, 289
TaqI TCGA 2 cut(s) 61, 223
TasI AATT 1 cut(s) 167
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 1 cut(s) 154
TscAI CASTG 1 cut(s) 456
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 1 cut(s) 454
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 3 cut(s) 23, 209, 252
TspRI CASTG 1 cut(s) 456
XapI RAATTY 1 cut(s) 167
XmnI GAANNNNTTC 2 cut(s) 243, 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.