pycom10g21740

MuDR family transposase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000885
Physical Location & Seq
Forward (+)
24314405 .. 24317146
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g21740.3

Sequence Viewer

Length: 1521 bp
ATGTACTCGGACCCAGTCCGCTCTCCAAACGACTCCGTCGGCTCCGCCGCGGGCGCCACCGCCCCATCGTCAGCAACACTCTGTAGCTTTCGCCGTTGGAAAATGACTAATTTGACAATTTTGATCAAGTATGATGGGAAGTGGGTGAATTCCTTATATAAGGGTGGCAAAACTAAGGCATTAGTTGTTTCTGAAAAACTTACTTATGAGGAGCTTCGAGATAGGCTGTATGGTCTCATGAAGGTCGACCCAAATGAATATGGAATTATAATGAAAGCTGTATATAGTGGAAAGTCTCATCCGTATCCTGCAGAAGTAGTAGATGATCACGACGTGGCCGCATTTATTTGTGAGAGTGTTGCACGGGACTGGAAAATTCCATTGTGCATTACTCTTGAACGCAGGGTATTAGCTTCTGGAAGTGGAAGTAATCAACAAGCTCCAATCGGCCTTGCAACTCATATTACAAAATCTCATTCATTAGATCCCCACACTCAACATGAAGAACAAAATTCCTTTGACTTCATTTCCAGTCGAATGCCAATGGACTTCAGTTCAGGGACTCAAGCAAATTCGGATTGTGTTGATGGATTGATCCATGATGATTTCGAAAATGATAAGGAGCATGATAATATAGAAGTTGATGTGGAACAAAACAACCTGCAACTTTCTGAGGTGCCTCAGCCAAACTTGCAATTTGATGATTTGCCTCATGACAGCGGGCGATTTGATAATATGTATCATGAAAATATGCAATTTTGTGATCATCATCTCAATCCTCCTAAATCTCTACCTCGAGCACGGTCTTGGCGTAAACTCACAGAAGAAATGGTTAATAGGATGCTGAATTCGGGGTTGAAAGCAGGGGGAGATATGGAACTAGGTTCAGTGTACAAGAGCAAAAAGGATTTACAGAAGATGTTAGCCCTTGCTGCAATTAAAAGAAGTTTTGAGTTCAAAGTTAAGAGGTCCACGAGAAAGAGATTTGCTGTTGAATGTGTGAACGACAACTGCACATGGAAAGTCCATGCAACCAAGGCGCCAAACTCTGATTATTTCATGATAACAAAATATGATAGTCGCCACTCTTGTTCATCAGATGCTAAAAATCTCAGTTCATTAGATGCAAAAAATGGCAGTCACCGACAAGCAAGCAGCTTGCTCATTGGTGAGTTTGTAAAGTCGAAATACAAGGGTGTTTTGTGCCAATGCAAACCGAAAAATATCATTGAGGATGTGCAGAAAGATCTTGGAGTGAATATAAGTTATTCGACAGCTAAGAGGGTTTTGGATTGTGCATTGAAAGATATTGGGGTATCATCACCAGAACTTTCTTCTAAACAGAGGTCAAGTGAAAGGTGCAAGAAGGAAAGAGCTCCGTCTCAACCCAAAAAAATGTCTTGTGGAGAAGAGCAGTCTACACGGAATTGTGGAAAATGTGGCTCAACTGGTCATTACCGTAAAACTTGCAAGAACCCTACTACTCTTCATCTTAATTCCGAAAGTGAGTTTGCTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut

Protein Analysis

507

Amino Acids

56.69

Weight (kDa)

8.53

Isoelectric Point (pI)

52.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 669
AccB1I GGYRCC 3 cut(s) 53, 676, 1039
AccBSI CCGCTC 1 cut(s) 21
AccI GTMKAC 2 cut(s) 246, 1418
AccII CGCG 1 cut(s) 50
AciI CCGC 7 cut(s) 19, 45, 48, 50, 60, 339, 720
AclWI GGATC 2 cut(s) 479, 589
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 5 cut(s) 148, 375, 511, 571, 847
AcuI CTGAAG 1 cut(s) 535
AcyI GRCGYC 2 cut(s) 54, 1040
AdeI CACNNNGTG 1 cut(s) 334
AfaI GTAC 2 cut(s) 5, 893
AgsI TTSAA 5 cut(s) 398, 859, 958, 995, 1303
AjiI CACGTC 1 cut(s) 334
AluBI AGCT 8 cut(s) 87, 214, 278, 413, 440, 1158, 1277, 1376
AluI AGCT 8 cut(s) 87, 214, 278, 413, 440, 1158, 1277, 1376
Alw21I GWGCWC 2 cut(s) 802, 1378
Alw26I GTCTC 3 cut(s) 239, 300, 1386
AlwI GGATC 2 cut(s) 479, 589
Ama87I CYCGRG 1 cut(s) 795
AoxI GGCC 2 cut(s) 336, 448
ApeKI GCWGC 3 cut(s) 932, 1155, 1514
ApoI RAATTY 5 cut(s) 148, 375, 511, 571, 847
AspLEI GCGC 2 cut(s) 56, 1042
AspS9I GGNCC 2 cut(s) 10, 969
AsuHPI GGTGA 4 cut(s) 157, 1133, 1181, 1314
AsuII TTCGAA 1 cut(s) 609
AvaI CYCGRG 1 cut(s) 795
AvaII GGWCC 2 cut(s) 10, 969
BanI GGYRCC 3 cut(s) 53, 676, 1039
BanII GRGCYC 1 cut(s) 1378
BauI CACGAG 1 cut(s) 973
Bbv12I GWGCWC 2 cut(s) 802, 1378
BbvCI CCTCAGC 1 cut(s) 681
BbvI GCAGC 3 cut(s) 919, 1167, 1501
BccI CCATC 3 cut(s) 73, 128, 581
BceAI ACGGC 1 cut(s) 78
BciVI GTATCC 1 cut(s) 315
BclI TGATCA 3 cut(s) 123, 325, 763
BcoDI GTCTC 3 cut(s) 239, 300, 1386
BfaI CTAG 2 cut(s) 881, 1519
BfmI CTRYAG 2 cut(s) 82, 309
BfoI RGCGCY 2 cut(s) 57, 1043
BfuAI ACCTGC 1 cut(s) 669
BfuI GTATCC 1 cut(s) 315
BglII AGATCT 1 cut(s) 1246
BisI GCNGC 5 cut(s) 48, 339, 933, 1156, 1515
BlsI GCNGC 5 cut(s) 49, 340, 934, 1157, 1516
Bme18I GGWCC 2 cut(s) 10, 969
BmeT110I CYCGRG 1 cut(s) 795
BmgBI CACGTC 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 10, 969
BmiI GGNNCC 5 cut(s) 12, 43, 55, 678, 1041
BmrI ACTGGG 1 cut(s) 8
BmsI GCATC 3 cut(s) 831, 1090, 1114
BmuI ACTGGG 1 cut(s) 8
Bpu10I CCTNAGC 1 cut(s) 681
Bpu14I TTCGAA 1 cut(s) 609
BpuEI CTTGAG 1 cut(s) 549
BsaBI GATNNNNATC 1 cut(s) 768
BsaHI GRCGYC 2 cut(s) 54, 1040
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 2 cut(s) 48, 1035
Bse1I ACTGG 4 cut(s) 14, 374, 531, 1453
Bse8I GATNNNNATC 1 cut(s) 768
BseDI CCNNGG 2 cut(s) 48, 1035
BseGI GGATG 3 cut(s) 298, 846, 1240
BseJI GATNNNNATC 1 cut(s) 768
BseMII CTCAG 3 cut(s) 663, 695, 1126
BseNI ACTGG 4 cut(s) 14, 374, 531, 1453
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 3 cut(s) 919, 1167, 1501
BsgI GTGCAG 2 cut(s) 997, 1259
Bsh1236I CGCG 1 cut(s) 50
BshFI GGCC 2 cut(s) 338, 450
BshNI GGYRCC 3 cut(s) 53, 676, 1039
BsiHKAI GWGCWC 2 cut(s) 802, 1378
BsiHKCI CYCGRG 1 cut(s) 795
BslFI GGGAC 2 cut(s) 380, 574
BsmAI GTCTC 3 cut(s) 239, 300, 1386
BsmBI CGTCTC 1 cut(s) 1386
BsmFI GGGAC 2 cut(s) 380, 574
BsmI GAATGC 1 cut(s) 543
BsnI GGCC 2 cut(s) 338, 450
Bso31I GGTCTC 1 cut(s) 239
BsoBI CYCGRG 1 cut(s) 795
Bsp119I TTCGAA 1 cut(s) 609
Bsp1286I GDGCHC 2 cut(s) 802, 1378
Bsp1407I TGTACA 1 cut(s) 891
Bsp143I GATC 6 cut(s) 123, 325, 484, 594, 763, 1246
BspACI CCGC 7 cut(s) 19, 45, 48, 50, 60, 339, 720
BspANI GGCC 2 cut(s) 338, 450
BspCNI CTCAG 3 cut(s) 664, 694, 1125
BspFNI CGCG 1 cut(s) 50
BspHI TCATGA 4 cut(s) 237, 712, 742, 1059
BspLI GGNNCC 5 cut(s) 12, 43, 55, 678, 1041
BspMAI CTGCAG 1 cut(s) 313
BspMI ACCTGC 1 cut(s) 669
BspPI GGATC 2 cut(s) 479, 589
BspQI GCTCTTC 1 cut(s) 1404
BspT104I TTCGAA 1 cut(s) 609
BspT107I GGYRCC 3 cut(s) 53, 676, 1039
BspTNI GGTCTC 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 21
BsrGI TGTACA 1 cut(s) 891
BsrI ACTGG 4 cut(s) 14, 374, 531, 1453
BssECI CCNNGG 2 cut(s) 48, 1035
BssMI GATC 6 cut(s) 123, 325, 484, 594, 763, 1246
BssNI GRCGYC 2 cut(s) 54, 1040
BssSI CACGAG 1 cut(s) 973
BssT1I CCWWGG 1 cut(s) 1035
Bst2BI CACGAG 1 cut(s) 973
Bst4CI ACNGT 2 cut(s) 804, 1460
Bst6I CTCTTC 2 cut(s) 1404, 1491
BstACI GRCGYC 2 cut(s) 54, 1040
BstAUI TGTACA 1 cut(s) 891
BstBI TTCGAA 1 cut(s) 609
BstC8I GCNNGC 4 cut(s) 52, 722, 1153, 1160
BstDEI CTNAG 5 cut(s) 174, 672, 681, 1112, 1278
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 3 cut(s) 298, 846, 1240
BstFNI CGCG 1 cut(s) 50
BstH2I RGCGCY 2 cut(s) 57, 1043
BstHHI GCGC 2 cut(s) 56, 1042
BstKTI GATC 6 cut(s) 126, 328, 487, 597, 766, 1249
BstMAI GTCTC 3 cut(s) 239, 300, 1386
BstMBI GATC 6 cut(s) 123, 325, 484, 594, 763, 1246
BstMWI GCNNNNNNNGC 4 cut(s) 53, 691, 932, 1037
BstSFI CTRYAG 2 cut(s) 82, 309
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 3 cut(s) 919, 1167, 1501
BstX2I RGATCY 2 cut(s) 484, 1246
BstYI RGATCY 2 cut(s) 484, 1246
BsuI GTATCC 1 cut(s) 315
BsuRI GGCC 2 cut(s) 338, 450
BtgI CCRYGG 1 cut(s) 48
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 3 cut(s) 298, 846, 1240
BtsIMutI CAGTG 1 cut(s) 894
BveI ACCTGC 1 cut(s) 669
Cac8I GCNNGC 4 cut(s) 52, 722, 1153, 1160
CciI TCATGA 4 cut(s) 237, 712, 742, 1059
CfoI GCGC 2 cut(s) 56, 1042
Cfr13I GGNCC 2 cut(s) 10, 969
Cfr42I CCGCGG 1 cut(s) 51
Csp6I GTAC 2 cut(s) 4, 892
CviAII CATG 9 cut(s) 238, 500, 599, 626, 713, 743, 1017, 1028, 1060
CviQI GTAC 2 cut(s) 4, 892
DdeI CTNAG 5 cut(s) 174, 672, 681, 1112, 1278
DinI GGCGCC 2 cut(s) 55, 1041
DpnI GATC 6 cut(s) 125, 327, 486, 596, 765, 1248
DpnII GATC 6 cut(s) 123, 325, 484, 594, 763, 1246
DraIII CACNNNGTG 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 336
Eam1104I CTCTTC 2 cut(s) 1404, 1491
EarI CTCTTC 2 cut(s) 1404, 1491
EciI GGCGGA 1 cut(s) 34
Ecl136II GAGCTC 1 cut(s) 1376
Eco130I CCWWGG 1 cut(s) 1035
Eco24I GRGCYC 1 cut(s) 1378
Eco31I GGTCTC 1 cut(s) 239
Eco47I GGWCC 2 cut(s) 10, 969
Eco53kI GAGCTC 1 cut(s) 1376
Eco57I CTGAAG 1 cut(s) 535
Eco88I CYCGRG 1 cut(s) 795
EcoICRI GAGCTC 1 cut(s) 1376
EcoRI GAATTC 2 cut(s) 148, 847
EcoT14I CCWWGG 1 cut(s) 1035
EcoT38I GRGCYC 1 cut(s) 1378
EgeI GGCGCC 2 cut(s) 55, 1041
EheI GGCGCC 2 cut(s) 55, 1041
ErhI CCWWGG 1 cut(s) 1035
Esp3I CGTCTC 1 cut(s) 1386
FaeI CATG 9 cut(s) 241, 503, 602, 629, 716, 746, 1020, 1031, 1063
FaqI GGGAC 2 cut(s) 380, 574
FatI CATG 9 cut(s) 237, 499, 598, 625, 712, 742, 1016, 1027, 1059
FauI CCCGC 2 cut(s) 43, 713
FbaI TGATCA 3 cut(s) 123, 325, 763
FblI GTMKAC 2 cut(s) 246, 1418
Fnu4HI GCNGC 5 cut(s) 48, 339, 933, 1156, 1515
FokI GGATG 3 cut(s) 285, 853, 1247
FriOI GRGCYC 1 cut(s) 1378
Fsp4HI GCNGC 5 cut(s) 48, 339, 933, 1156, 1515
FspBI CTAG 2 cut(s) 881, 1519
GlaI GCGC 2 cut(s) 55, 1041
GluI GCNGC 5 cut(s) 48, 339, 933, 1156, 1515
HaeII RGCGCY 2 cut(s) 57, 1043
HaeIII GGCC 2 cut(s) 338, 450
HhaI GCGC 2 cut(s) 56, 1042
Hin1I GRCGYC 2 cut(s) 54, 1040
Hin1II CATG 9 cut(s) 241, 503, 602, 629, 716, 746, 1020, 1031, 1063
Hin6I GCGC 2 cut(s) 54, 1040
HinP1I GCGC 2 cut(s) 54, 1040
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 2 cut(s) 32, 562
HphI GGTGA 4 cut(s) 157, 1133, 1181, 1314
Hpy166II GTNNAC 6 cut(s) 247, 815, 892, 972, 1003, 1419
Hpy188I TCNGA 7 cut(s) 10, 193, 577, 673, 1051, 1099, 1501
Hpy188III TCNNGA 8 cut(s) 218, 238, 329, 395, 417, 713, 743, 1060
Hpy8I GTNNAC 6 cut(s) 247, 815, 892, 972, 1003, 1419
Hpy99I CGWCG 2 cut(s) 41, 335
HpyAV CCTTC 2 cut(s) 235, 1360
HpyCH4III ACNGT 2 cut(s) 804, 1460
HpyCH4IV ACGT 1 cut(s) 333
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 691, 932, 1037
HpyF3I CTNAG 5 cut(s) 174, 672, 681, 1112, 1278
HpySE526I ACGT 1 cut(s) 333
Hsp92I GRCGYC 2 cut(s) 54, 1040
Hsp92II CATG 9 cut(s) 241, 503, 602, 629, 716, 746, 1020, 1031, 1063
HspAI GCGC 2 cut(s) 54, 1040
KasI GGCGCC 2 cut(s) 53, 1039
Ksp22I TGATCA 3 cut(s) 123, 325, 763
KspI CCGCGG 1 cut(s) 51
Kzo9I GATC 6 cut(s) 123, 325, 484, 594, 763, 1246
LguI GCTCTTC 1 cut(s) 1404
LmnI GCTCC 5 cut(s) 47, 211, 445, 622, 1381
Lsp1109I GCAGC 3 cut(s) 919, 1167, 1501
LweI GCATC 3 cut(s) 831, 1090, 1114
MaeI CTAG 2 cut(s) 881, 1519
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 1139
MalI GATC 6 cut(s) 125, 327, 486, 596, 765, 1248
MbiI CCGCTC 1 cut(s) 21
MboI GATC 6 cut(s) 123, 325, 484, 594, 763, 1246
MboII GAAGA 6 cut(s) 515, 836, 928, 1326, 1421, 1478
MflI RGATCY 2 cut(s) 484, 1246
MhlI GDGCHC 2 cut(s) 802, 1378
Mly113I GGCGCC 2 cut(s) 54, 1040
MlyI GAGTC 2 cut(s) 26, 556
MmeI TCCRAC 1 cut(s) 77
MseI TTAA 4 cut(s) 834, 939, 963, 1494
MspA1I CMGCKG 2 cut(s) 50, 720
Mva1269I GAATGC 1 cut(s) 543
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 4 cut(s) 53, 691, 932, 1037
NarI GGCGCC 2 cut(s) 54, 1040
NdeII GATC 6 cut(s) 123, 325, 484, 594, 763, 1246
NlaIII CATG 9 cut(s) 241, 503, 602, 629, 716, 746, 1020, 1031, 1063
NlaIV GGNNCC 5 cut(s) 12, 43, 55, 678, 1041
NmuCI GTSAC 1 cut(s) 1139
NspV TTCGAA 1 cut(s) 609
PaeR7I CTCGAG 1 cut(s) 795
PagI TCATGA 4 cut(s) 237, 712, 742, 1059
PciSI GCTCTTC 1 cut(s) 1404
PcsI WCGNNNNNNNCGW 1 cut(s) 808
PctI GAATGC 1 cut(s) 543
PflFI GACNNNGTC 2 cut(s) 14, 35
PkrI GCNGC 5 cut(s) 49, 340, 934, 1157, 1516
PleI GAGTC 2 cut(s) 26, 556
PluTI GGCGCC 2 cut(s) 57, 1043
PpsI GAGTC 2 cut(s) 26, 556
PsiI TTATAA 1 cut(s) 269
Psp124BI GAGCTC 1 cut(s) 1378
PspN4I GGNNCC 5 cut(s) 12, 43, 55, 678, 1041
PspPI GGNCC 2 cut(s) 10, 969
PspXI VCTCGAGB 1 cut(s) 795
PstI CTGCAG 1 cut(s) 313
PsuI RGATCY 2 cut(s) 484, 1246
PsyI GACNNNGTC 2 cut(s) 14, 35
RsaI GTAC 2 cut(s) 5, 893
RsaNI GTAC 2 cut(s) 4, 892
SacI GAGCTC 1 cut(s) 1378
SacII CCGCGG 1 cut(s) 51
SalI GTCGAC 1 cut(s) 245
SapI GCTCTTC 1 cut(s) 1404
SaqAI TTAA 4 cut(s) 834, 939, 963, 1494
SatI GCNGC 5 cut(s) 48, 339, 933, 1156, 1515
Sau3AI GATC 6 cut(s) 123, 325, 484, 594, 763, 1246
Sau96I GGNCC 2 cut(s) 10, 969
SchI GAGTC 2 cut(s) 26, 556
SduI GDGCHC 2 cut(s) 802, 1378
SfaNI GCATC 3 cut(s) 831, 1090, 1114
SfcI CTRYAG 2 cut(s) 82, 309
SfoI GGCGCC 2 cut(s) 55, 1041
Sfr274I CTCGAG 1 cut(s) 795
Sfr303I CCGCGG 1 cut(s) 51
SfuI TTCGAA 1 cut(s) 609
SgrBI CCGCGG 1 cut(s) 51
SinI GGWCC 2 cut(s) 10, 969
SlaI CTCGAG 1 cut(s) 795
SmlI CTYRAG 2 cut(s) 564, 795
SmoI CTYRAG 2 cut(s) 564, 795
SsiI CCGC 7 cut(s) 19, 45, 48, 50, 60, 339, 720
SspDI GGCGCC 2 cut(s) 53, 1039
SspMI CTAG 2 cut(s) 881, 1519
SstI GAGCTC 1 cut(s) 1378
StyI CCWWGG 1 cut(s) 1035
TaaI ACNGT 2 cut(s) 804, 1460
TaiI ACGT 1 cut(s) 336
TaqI TCGA 7 cut(s) 217, 246, 535, 609, 796, 1184, 1271
TatI WGTACW 2 cut(s) 3, 891
TauI GCSGC 2 cut(s) 50, 341
Tru1I TTAA 4 cut(s) 834, 939, 963, 1494
Tru9I TTAA 4 cut(s) 834, 939, 963, 1494
TscAI CASTG 1 cut(s) 894
TseFI GTSAC 1 cut(s) 1139
TseI GCWGC 3 cut(s) 932, 1155, 1514
Tsp45I GTSAC 1 cut(s) 1139
TspGWI ACGGA 4 cut(s) 25, 291, 1368, 1438
TspRI CASTG 1 cut(s) 894
Tth111I GACNNNGTC 2 cut(s) 14, 35
VpaK11BI GGWCC 2 cut(s) 10, 969
XapI RAATTY 5 cut(s) 148, 375, 511, 571, 847
XhoI CTCGAG 1 cut(s) 795
XmiI GTMKAC 2 cut(s) 246, 1418
XspI CTAG 2 cut(s) 881, 1519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.