Rh5DG247800

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000885
Physical Location & Seq
Reverse (-)
28433341 .. 28437661
4321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG247800.1

Sequence Viewer

Length: 1377 bp
ATGAATTTGAGCTCAAGGAGGCTTCCTCCGATGGACTACCACCACCGCAAGGAGACTCGGCCTTCCTGTCCGCCACTCTGGCCGGCTTCTTCATCCTCAGAAACCCCAATTAACACCGTCCGCGAACAAATCACAAAGTGCCTCGAAATCGTCAGGGCCGGACCACCAAGAGAATTTTCATCCCTTTCCGAGCTTCCCCGATATATTCCACGTGGAGTCCTTCTGGGTAATCTTGACATACGGAAGGATCCAGTTGGACCAGTGAAAGCTTCAATTTCGGCCGACGGGCGAGGTGTCATATTGGAAATTATTGGAGCACCAGGAGCTTATATATACGTTGTGACTAAACGTTTGGCACGGTCGGAGAGGGCATTGGATGAGCTCTCGGAGGTTGTAGAAGAAAAAACAGTGCTGCCAATATTCCACGGTGTTGATCCCTCCGATGTACGACATCAAACGGGAAGTTTTGCACAAGCCTTTGCAGACCATGAGATAAAGTACAAGGATGACCCACAAAAAGTTAAAAGGTGGAGAGCTGCTTTAACCAAACTGGGAAATCTTGCAGGCTGGCGTTCAAAGGGCTGTCACGAAACAAAACTCATTCAAGACGTTGTGGATGAGATACGGTGGAAATTAAAAGCATCTAAAGTGGGAAGGAAGAATGAAACTTTGATGAAGCCATCTGCAAAATATGAAGAACAAGCTCATACTCTGGTGAATGGAGACAAAACACATAAGGGGAAGGCTAGTTTGCGGAACAAAGAAAACATCACTGATCATAATAAAAGGAGGAGTATTCAAATAATCGATGATGAAAGCCTTGCATTTTTCATCCATGATGAGAATCTTTCACTGGGTATGAGCTCCATATTTACTTTGTGCACTACTCTTGAACGACGGGCATTTCCTAATGGACGGATGGAGTCCTCACATAGTGGTACACCACATCTCAGAAGATTTGGGACCTACCCGAGTTGCGTTCATTGTGTGACACCTGAACAGGTGCCTCATCTTCATACTCAAGTTGGACCATCGTCACCAAATGTGCCTTGTATGCAGCAGCAGCAGCAACAACCTTTCTACCAGCAACAACAACAACAACCTTTCTACCAGCAACAACATTCTTACCAACAGACGACGTTGCAGCCTTGCTACGAGCACTATCAACCATCCGTTCAGCAGCCACAGCCTCTGTACCAACAACTTCATCCTTCCTACGTGCAGCAGCAGCAGTCTCAGTACCAACAGCCGAATCCTTCCTACGTGCAGCAGCAGCAGTCTCAGTACCAACAGCCGAATCCTTCCTACATGCAGCCGCTGTCACCAATTCATCATGTACCTTACTCGCTCAAGATGTTCTGGAATGATGCAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

458

Amino Acids

52.46

Weight (kDa)

9.07

Isoelectric Point (pI)

69.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 100 - 220 2e-18 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1003
AccII CGCG 1 cut(s) 123
AciI CCGC 5 cut(s) 46, 71, 121, 754, 1316
AclI AACGTT 1 cut(s) 349
AclWI GGATC 3 cut(s) 242, 255, 428
AcoI YGGCCR 2 cut(s) 80, 279
AcsI RAATTY 2 cut(s) 4, 173
AcvI CACGTG 1 cut(s) 212
AdeI CACNNNGTG 2 cut(s) 138, 935
AfaI GTAC 7 cut(s) 447, 500, 940, 1196, 1241, 1286, 1338
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 5 cut(s) 273, 576, 605, 800, 893
AjnI CCWGG 1 cut(s) 319
AjuI GAANNNNNNNTTGG 2 cut(s) 160, 192
AluBI AGCT 8 cut(s) 12, 193, 269, 326, 382, 536, 704, 864
AluI AGCT 8 cut(s) 12, 193, 269, 326, 382, 536, 704, 864
Alw21I GWGCWC 6 cut(s) 14, 319, 384, 866, 884, 1161
Alw26I GTCTC 4 cut(s) 47, 717, 1239, 1284
Alw44I GTGCAC 1 cut(s) 880
AlwI GGATC 3 cut(s) 242, 255, 428
AlwNI CAGNNNCTG 2 cut(s) 1192, 1318
Ama87I CYCGRG 1 cut(s) 970
AoxI GGCC 4 cut(s) 59, 80, 156, 279
ApaLI GTGCAC 1 cut(s) 880
ApoI RAATTY 2 cut(s) 4, 173
ArsI GACNNNNNNTTYG 2 cut(s) 334, 366
AspS9I GGNCC 5 cut(s) 156, 161, 257, 963, 1028
AsuHPI GGTGA 3 cut(s) 727, 1029, 1314
AvaI CYCGRG 1 cut(s) 970
AvaII GGWCC 4 cut(s) 161, 257, 963, 1028
BaeGI GKGCMC 1 cut(s) 884
BamHI GGATCC 1 cut(s) 247
BanI GGYRCC 1 cut(s) 1003
BanII GRGCYC 3 cut(s) 14, 384, 866
BbrPI CACGTG 1 cut(s) 212
Bbv12I GWGCWC 6 cut(s) 14, 319, 384, 866, 884, 1161
BccI CCATC 5 cut(s) 25, 688, 913, 1039, 1177
BciT130I CCWGG 1 cut(s) 321
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 4 cut(s) 47, 717, 1239, 1284
BfaI CTAG 1 cut(s) 747
BglI GCCNNNNNGGC 1 cut(s) 79
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 4 cut(s) 161, 257, 963, 1028
BmeT110I CYCGRG 1 cut(s) 970
BmgT120I GGNCC 5 cut(s) 156, 161, 257, 963, 1028
BmiI GGNNCC 3 cut(s) 249, 964, 1005
BmrFI CCNGG 1 cut(s) 321
BmrI ACTGGG 2 cut(s) 560, 863
BmsI GCATC 2 cut(s) 650, 1357
BmuI ACTGGG 2 cut(s) 560, 863
BoxI GACNNNNGTC 1 cut(s) 1033
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpuEI CTTGAG 2 cut(s) 1005, 1334
Bsa29I ATCGAT 1 cut(s) 807
BsaAI YACGTR 3 cut(s) 212, 1219, 1264
BsaBI GATNNNNATC 1 cut(s) 843
BsaJI CCNNGG 1 cut(s) 424
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 4 cut(s) 251, 260, 555, 858
Bse8I GATNNNNATC 1 cut(s) 843
BseBI CCWGG 1 cut(s) 321
BseCI ATCGAT 1 cut(s) 807
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 9 cut(s) 92, 179, 382, 511, 622, 831, 924, 1169, 1207
BseJI GATNNNNATC 1 cut(s) 843
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMII CTCAG 4 cut(s) 111, 964, 1250, 1295
BseNI ACTGG 4 cut(s) 251, 260, 555, 858
BseRI GAGGAG 1 cut(s) 805
BseSI GKGCMC 1 cut(s) 884
BseX3I CGGCCG 1 cut(s) 279
BsgI GTGCAG 2 cut(s) 1241, 1286
Bsh1236I CGCG 1 cut(s) 123
Bsh1285I CGRYCG 2 cut(s) 282, 362
BshFI GGCC 4 cut(s) 61, 82, 158, 281
BshNI GGYRCC 1 cut(s) 1003
BshVI ATCGAT 1 cut(s) 807
BsiEI CGRYCG 2 cut(s) 282, 362
BsiHKAI GWGCWC 6 cut(s) 14, 319, 384, 866, 884, 1161
BsiHKCI CYCGRG 1 cut(s) 970
BsiSI CCGG 2 cut(s) 83, 159
BslFI GGGAC 1 cut(s) 976
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 4 cut(s) 47, 717, 1239, 1284
BsmFI GGGAC 1 cut(s) 976
BsnI GGCC 4 cut(s) 61, 82, 158, 281
BsoBI CYCGRG 1 cut(s) 970
Bsp1286I GDGCHC 6 cut(s) 14, 319, 384, 866, 884, 1161
Bsp143I GATC 3 cut(s) 247, 433, 775
BspACI CCGC 5 cut(s) 46, 71, 121, 754, 1316
BspANI GGCC 4 cut(s) 61, 82, 158, 281
BspCNI CTCAG 4 cut(s) 110, 963, 1249, 1294
BspDI ATCGAT 1 cut(s) 807
BspFNI CGCG 1 cut(s) 123
BspLI GGNNCC 3 cut(s) 249, 964, 1005
BspPI GGATC 3 cut(s) 242, 255, 428
BspT107I GGYRCC 1 cut(s) 1003
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 4 cut(s) 251, 260, 555, 858
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 1 cut(s) 424
BssMI GATC 3 cut(s) 247, 433, 775
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 5 cut(s) 118, 360, 409, 428, 627
BstBAI YACGTR 3 cut(s) 212, 1219, 1264
BstC8I GCNNGC 3 cut(s) 84, 565, 569
BstDEI CTNAG 4 cut(s) 97, 950, 1236, 1281
BstDSI CCRYGG 1 cut(s) 424
BstF5I GGATG 9 cut(s) 92, 179, 382, 511, 622, 831, 924, 1169, 1207
BstFNI CGCG 1 cut(s) 123
BstKTI GATC 3 cut(s) 250, 436, 778
BstMAI GTCTC 4 cut(s) 47, 717, 1239, 1284
BstMBI GATC 3 cut(s) 247, 433, 775
BstMCI CGRYCG 2 cut(s) 282, 362
BstMWI GCNNNNNNNGC 8 cut(s) 79, 323, 1054, 1063, 1066, 1186, 1228, 1273
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 1 cut(s) 1312
BstPAI GACNNNNGTC 1 cut(s) 1033
BstSCI CCNGG 1 cut(s) 319
BstSLI GKGCMC 1 cut(s) 884
BstUI CGCG 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BstZI CGGCCG 1 cut(s) 279
Bsu15I ATCGAT 1 cut(s) 807
BsuRI GGCC 4 cut(s) 61, 82, 158, 281
BsuTUI ATCGAT 1 cut(s) 807
BtgI CCRYGG 1 cut(s) 424
BtsCI GGATG 9 cut(s) 92, 179, 382, 511, 622, 831, 924, 1169, 1207
BtsIMutI CAGTG 4 cut(s) 267, 414, 771, 851
Cac8I GCNNGC 3 cut(s) 84, 565, 569
CaiI CAGNNNCTG 2 cut(s) 1192, 1318
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 5 cut(s) 156, 161, 257, 963, 1028
ClaI ATCGAT 1 cut(s) 807
Csp6I GTAC 7 cut(s) 446, 499, 939, 1195, 1240, 1285, 1337
CviAII CATG 4 cut(s) 488, 836, 1309, 1334
CviQI GTAC 7 cut(s) 446, 499, 939, 1195, 1240, 1285, 1337
DdeI CTNAG 4 cut(s) 97, 950, 1236, 1281
DpnI GATC 3 cut(s) 249, 435, 777
DpnII GATC 3 cut(s) 247, 433, 775
DraIII CACNNNGTG 2 cut(s) 138, 935
EaeI YGGCCR 2 cut(s) 80, 279
EagI CGGCCG 1 cut(s) 279
EciI GGCGGA 1 cut(s) 60
Ecl136II GAGCTC 3 cut(s) 12, 382, 864
EclXI CGGCCG 1 cut(s) 279
Eco24I GRGCYC 3 cut(s) 14, 384, 866
Eco47I GGWCC 4 cut(s) 161, 257, 963, 1028
Eco52I CGGCCG 1 cut(s) 279
Eco53kI GAGCTC 3 cut(s) 12, 382, 864
Eco72I CACGTG 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 970
EcoICRI GAGCTC 3 cut(s) 12, 382, 864
EcoO109I RGGNCCY 1 cut(s) 963
EcoRII CCWGG 1 cut(s) 319
EcoT38I GRGCYC 3 cut(s) 14, 384, 866
FaeI CATG 4 cut(s) 491, 839, 1312, 1337
FaqI GGGAC 1 cut(s) 976
FatI CATG 4 cut(s) 487, 835, 1308, 1333
FbaI TGATCA 1 cut(s) 775
FokI GGATG 9 cut(s) 79, 166, 389, 518, 629, 818, 931, 1156, 1194
FriOI GRGCYC 3 cut(s) 14, 384, 866
FspBI CTAG 1 cut(s) 747
HaeIII GGCC 4 cut(s) 61, 82, 158, 281
HapII CCGG 2 cut(s) 83, 159
Hin1II CATG 4 cut(s) 491, 839, 1312, 1337
HindIII AAGCTT 1 cut(s) 267
HinfI GANTC 6 cut(s) 55, 216, 844, 923, 1252, 1297
HpaII CCGG 2 cut(s) 83, 159
HphI GGTGA 3 cut(s) 727, 1029, 1314
Hpy166II GTNNAC 2 cut(s) 882, 941
Hpy188I TCNGA 7 cut(s) 30, 100, 190, 364, 388, 442, 953
Hpy188III TCNNGA 6 cut(s) 233, 587, 605, 890, 1351, 1360
Hpy8I GTNNAC 2 cut(s) 882, 941
Hpy99I CGWCG 3 cut(s) 287, 900, 1141
HpyAV CCTTC 8 cut(s) 72, 230, 238, 648, 736, 1221, 1266, 1311
HpyCH4III ACNGT 5 cut(s) 118, 360, 409, 428, 627
HpyCH4IV ACGT 7 cut(s) 211, 336, 349, 609, 1139, 1218, 1263
HpyF10VI GCNNNNNNNGC 8 cut(s) 79, 323, 1054, 1063, 1066, 1186, 1228, 1273
HpyF3I CTNAG 4 cut(s) 97, 950, 1236, 1281
HpySE526I ACGT 7 cut(s) 211, 336, 349, 609, 1139, 1218, 1263
Hsp92II CATG 4 cut(s) 491, 839, 1312, 1337
KroI GCCGGC 1 cut(s) 82
KroNI GCCGGC 1 cut(s) 84
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 3 cut(s) 247, 433, 775
LmnI GCTCC 3 cut(s) 314, 323, 869
LweI GCATC 2 cut(s) 650, 1357
MaeI CTAG 1 cut(s) 747
MaeII ACGT 7 cut(s) 211, 336, 349, 609, 1139, 1218, 1263
MaeIII GTNAC 5 cut(s) 340, 584, 988, 1035, 1320
MalI GATC 3 cut(s) 249, 435, 777
MboI GATC 3 cut(s) 247, 433, 775
MboII GAAGA 6 cut(s) 81, 410, 670, 707, 966, 1004
MflI RGATCY 1 cut(s) 247
MhlI GDGCHC 6 cut(s) 14, 319, 384, 866, 884, 1161
MluCI AATT 7 cut(s) 4, 108, 173, 273, 306, 632, 1326
MlyI GAGTC 3 cut(s) 49, 225, 932
MmeI TCCRAC 3 cut(s) 235, 342, 1006
MroNI GCCGGC 1 cut(s) 82
MseI TTAA 4 cut(s) 111, 522, 542, 635
MspA1I CMGCKG 1 cut(s) 1318
MspI CCGG 2 cut(s) 83, 159
MspR9I CCNGG 1 cut(s) 321
MvaI CCWGG 1 cut(s) 321
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 8 cut(s) 79, 323, 1054, 1063, 1066, 1186, 1228, 1273
NaeI GCCGGC 1 cut(s) 84
NdeII GATC 3 cut(s) 247, 433, 775
NgoMIV GCCGGC 1 cut(s) 82
NlaIII CATG 4 cut(s) 491, 839, 1312, 1337
NlaIV GGNNCC 3 cut(s) 249, 964, 1005
NmeAIII GCCGAG 1 cut(s) 37
NmuCI GTSAC 5 cut(s) 340, 584, 988, 1035, 1320
NspI RCATGY 1 cut(s) 1312
PdiI GCCGGC 1 cut(s) 84
PfeI GAWTC 3 cut(s) 844, 1252, 1297
PleI GAGTC 3 cut(s) 49, 224, 931
PmaCI CACGTG 1 cut(s) 212
PmlI CACGTG 1 cut(s) 212
PpsI GAGTC 3 cut(s) 49, 224, 931
Ppu21I YACGTR 3 cut(s) 212, 1219, 1264
PpuMI RGGWCCY 1 cut(s) 963
PshAI GACNNNNGTC 1 cut(s) 1033
Psp124BI GAGCTC 3 cut(s) 14, 384, 866
Psp1406I AACGTT 1 cut(s) 349
Psp5II RGGWCCY 1 cut(s) 963
Psp6I CCWGG 1 cut(s) 319
PspCI CACGTG 1 cut(s) 212
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 3 cut(s) 249, 964, 1005
PspPI GGNCC 5 cut(s) 156, 161, 257, 963, 1028
PspPPI RGGWCCY 1 cut(s) 963
PstNI CAGNNNCTG 2 cut(s) 1192, 1318
PsuI RGATCY 1 cut(s) 247
RsaI GTAC 7 cut(s) 447, 500, 940, 1196, 1241, 1286, 1338
RsaNI GTAC 7 cut(s) 446, 499, 939, 1195, 1240, 1285, 1337
SacI GAGCTC 3 cut(s) 14, 384, 866
SaqAI TTAA 4 cut(s) 111, 522, 542, 635
Sau3AI GATC 3 cut(s) 247, 433, 775
Sau96I GGNCC 5 cut(s) 156, 161, 257, 963, 1028
SchI GAGTC 3 cut(s) 49, 225, 932
ScrFI CCNGG 1 cut(s) 321
SduI GDGCHC 6 cut(s) 14, 319, 384, 866, 884, 1161
SfaNI GCATC 2 cut(s) 650, 1357
SinI GGWCC 4 cut(s) 161, 257, 963, 1028
SmlI CTYRAG 3 cut(s) 13, 1020, 1349
SmoI CTYRAG 3 cut(s) 13, 1020, 1349
Sse9I AATT 7 cut(s) 4, 108, 173, 273, 306, 632, 1326
SsiI CCGC 5 cut(s) 46, 71, 121, 754, 1316
SspI AATATT 1 cut(s) 420
SspMI CTAG 1 cut(s) 747
SstI GAGCTC 3 cut(s) 14, 384, 866
StyD4I CCNGG 1 cut(s) 319
TaaI ACNGT 5 cut(s) 118, 360, 409, 428, 627
TaiI ACGT 7 cut(s) 214, 339, 352, 612, 1142, 1221, 1266
TaqI TCGA 2 cut(s) 144, 807
TasI AATT 7 cut(s) 4, 108, 173, 273, 306, 632, 1326
TatI WGTACW 1 cut(s) 498
TauI GCSGC 1 cut(s) 1318
TfiI GAWTC 3 cut(s) 844, 1252, 1297
Tru1I TTAA 4 cut(s) 111, 522, 542, 635
Tru9I TTAA 4 cut(s) 111, 522, 542, 635
TscAI CASTG 4 cut(s) 267, 414, 778, 858
TseFI GTSAC 5 cut(s) 340, 584, 988, 1035, 1320
Tsp45I GTSAC 5 cut(s) 340, 584, 988, 1035, 1320
TspGWI ACGGA 3 cut(s) 256, 931, 1162
TspRI CASTG 4 cut(s) 267, 414, 778, 858
VneI GTGCAC 1 cut(s) 880
VpaK11BI GGWCC 4 cut(s) 161, 257, 963, 1028
XapI RAATTY 2 cut(s) 4, 173
XceI RCATGY 1 cut(s) 1312
XspI CTAG 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.