Rroxscaffold_1G00044790

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000885
Physical Location & Seq
Forward (+)
63804201 .. 63805698
1498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00044790.1

Sequence Viewer

Length: 1074 bp
ATGGGTGAGTTGAGACTCGGCGTTAATTATGGCGGAAAGTGGGTTGGTTCTGTCTACATCGGTGGTAGTTCAGAAGAAATAGTGGTTTCAGAAGACATCACTTATAGTGAGCTTCTAGATAAACTGTATCCTATTGTTGGATTGCATCGAAATGAAGATGAGATCAAGATCAGTACGATTGATGAATCAAAATCACCAGCTTATCCTGTGGAGATAATCAATGATGATGACCTCAAAGCTTTTATTGACCAGAGCCTTTCTTCCGATATGCACTTGAAGCATCCATTGCGGAATTCCTCTAGGAGGAGGACTGAGGTTAGAGTTTTGGTTAATTATGATGGGGAGTGGGTGAATTCTACATACGTTGGCGGCAAAACGAAAGGAATTGTAATTTCAGACGATATGGCGCATCAAGAACTTGTGGATATATTGTATGGTATAGTGGGCGTTGATCCAAATGAATATAAGCTGATTATGAAGACCGCATATGAATCAAATCTACCTACTCAACCTGTGGAGATAATCGATGACAATGACCTTGCATTTTTCACTCATAATGAGAGTCATTCACTGGGCATGGGCTCCATATTCACTTTGTGCACTACTCTTGAACGGCGGGCATTTTCTAATGGAAGGATGGGGTCATCACAAAGTAGTGATACAGCACATCTTAGAAGATTTGGGACCTGTCAGAGTTGCGTTCATTGTGTTACTACACCTGATCAGGTGCCTTATCTTCGTACTCAAGTTGGACCATCATCACCAAATATGCCTTGTATGCAGCAGCAACAGCCTCCCTACCAACAACAACAACATTCTTACCAACAAACGACATCGCAGCCTTTCTACGAGCACTATCAGCCATGCTTTCAACGGCCGAAGTCTCCGTATCAACAACTTTATCCTTCCTACATGCAGCCGCCGCCACCAATTCATCATGTGCCTTATTCACTCAAGATGTTCTGGAATGATGCAGCCCGGATATTTCAAGATTTCAACTTTTGGCAATTTAGTTTCGTCATCATTTTATTAGGAAATATTTTCTATTTCTTAAGTCTTTGTAGAAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

357

Amino Acids

40.87

Weight (kDa)

5.52

Isoelectric Point (pI)

58.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 727
AccI GTMKAC 1 cut(s) 54
AciI CCGC 7 cut(s) 33, 289, 369, 483, 616, 920, 923
AclWI GGATC 1 cut(s) 446
AcoI YGGCCR 1 cut(s) 875
AcsI RAATTY 2 cut(s) 292, 352
AdeI CACNNNGTG 1 cut(s) 597
AfaI GTAC 2 cut(s) 175, 742
AfiI CCNNNNNNNGG 2 cut(s) 137, 303
AflII CTTAAG 1 cut(s) 1051
AgsI TTSAA 5 cut(s) 277, 611, 872, 989, 997
AluBI AGCT 4 cut(s) 112, 200, 239, 469
AluI AGCT 4 cut(s) 112, 200, 239, 469
Alw21I GWGCWC 2 cut(s) 602, 855
Alw26I GTCTC 2 cut(s) 7, 888
Alw44I GTGCAC 1 cut(s) 598
AlwI GGATC 1 cut(s) 446
AoxI GGCC 1 cut(s) 875
ApaLI GTGCAC 1 cut(s) 598
ApeKI GCWGC 5 cut(s) 781, 784, 838, 916, 974
ApoI RAATTY 2 cut(s) 292, 352
AspLEI GCGC 1 cut(s) 409
AspS9I GGNCC 2 cut(s) 684, 752
AsuC2I CCSGG 1 cut(s) 979
AsuHPI GGTGA 4 cut(s) 17, 186, 361, 753
AvaII GGWCC 2 cut(s) 684, 752
BaeGI GKGCMC 1 cut(s) 602
BanI GGYRCC 1 cut(s) 727
BanII GRGCYC 1 cut(s) 584
BbsI GAAGAC 2 cut(s) 99, 485
Bbv12I GWGCWC 2 cut(s) 602, 855
BbvI GCAGC 5 cut(s) 793, 796, 850, 928, 986
BccI CCATC 3 cut(s) 332, 631, 763
BceAI ACGGC 2 cut(s) 629, 890
BciVI GTATCC 1 cut(s) 138
BclI TGATCA 1 cut(s) 721
BcnI CCSGG 1 cut(s) 979
BcoDI GTCTC 2 cut(s) 7, 888
BfaI CTAG 2 cut(s) 116, 300
BfrI CTTAAG 1 cut(s) 1051
BfuI GTATCC 1 cut(s) 138
BisI GCNGC 8 cut(s) 370, 782, 785, 839, 917, 920, 923, 975
BlsI GCNGC 8 cut(s) 371, 783, 786, 840, 918, 921, 924, 976
Bme1390I CCNGG 1 cut(s) 979
Bme18I GGWCC 2 cut(s) 684, 752
BmgT120I GGNCC 2 cut(s) 684, 752
BmiI GGNNCC 3 cut(s) 583, 685, 729
BmrFI CCNGG 1 cut(s) 979
BmrI ACTGGG 1 cut(s) 581
BmsI GCATC 4 cut(s) 154, 289, 418, 961
BmuI ACTGGG 1 cut(s) 581
BpiI GAAGAC 2 cut(s) 99, 485
BpuEI CTTGAG 2 cut(s) 729, 938
BpuMI CCSGG 1 cut(s) 979
Bsa29I ATCGAT 1 cut(s) 525
BsaBI GATNNNNATC 1 cut(s) 167
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 303
Bse1I ACTGG 1 cut(s) 576
Bse3DI GCAATG 1 cut(s) 284
Bse8I GATNNNNATC 1 cut(s) 167
BseCI ATCGAT 1 cut(s) 525
BseGI GGATG 2 cut(s) 280, 642
BseJI GATNNNNATC 1 cut(s) 167
BseLI CCNNNNNNNGG 2 cut(s) 137, 303
BseMI GCAATG 1 cut(s) 284
BseMII CTCAG 1 cut(s) 303
BseNI ACTGG 1 cut(s) 576
BseRI GAGGAG 1 cut(s) 319
BseSI GKGCMC 1 cut(s) 602
BseX3I CGGCCG 1 cut(s) 875
BseXI GCAGC 5 cut(s) 793, 796, 850, 928, 986
Bsh1285I CGRYCG 1 cut(s) 878
BshFI GGCC 1 cut(s) 877
BshNI GGYRCC 1 cut(s) 727
BshVI ATCGAT 1 cut(s) 525
BsiEI CGRYCG 1 cut(s) 878
BsiHKAI GWGCWC 2 cut(s) 602, 855
BsiSI CCGG 1 cut(s) 979
BslFI GGGAC 1 cut(s) 697
BslI CCNNNNNNNGG 2 cut(s) 137, 303
BsmAI GTCTC 2 cut(s) 7, 888
BsmFI GGGAC 1 cut(s) 697
BsnI GGCC 1 cut(s) 877
Bsp1286I GDGCHC 3 cut(s) 584, 602, 855
Bsp143I GATC 4 cut(s) 162, 168, 451, 721
BspACI CCGC 7 cut(s) 33, 289, 369, 483, 616, 920, 923
BspANI GGCC 1 cut(s) 877
BspCNI CTCAG 1 cut(s) 304
BspDI ATCGAT 1 cut(s) 525
BspLI GGNNCC 3 cut(s) 583, 685, 729
BspPI GGATC 1 cut(s) 446
BspT107I GGYRCC 1 cut(s) 727
BspTI CTTAAG 1 cut(s) 1051
BsrDI GCAATG 1 cut(s) 284
BsrI ACTGG 1 cut(s) 576
BssMI GATC 4 cut(s) 162, 168, 451, 721
Bst4CI ACNGT 1 cut(s) 126
BstAFI CTTAAG 1 cut(s) 1051
BstAPI GCANNNNNTGC 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 2 cut(s) 312, 671
BstENI CCTNNNNNAGG 1 cut(s) 301
BstF5I GGATG 2 cut(s) 280, 642
BstHHI GCGC 1 cut(s) 409
BstKTI GATC 4 cut(s) 165, 171, 454, 724
BstMAI GTCTC 2 cut(s) 7, 888
BstMBI GATC 4 cut(s) 162, 168, 451, 721
BstMCI CGRYCG 1 cut(s) 878
BstMWI GCNNNNNNNGC 6 cut(s) 277, 286, 778, 790, 859, 922
BstNSI RCATGY 1 cut(s) 916
BstSCI CCNGG 1 cut(s) 977
BstSLI GKGCMC 1 cut(s) 602
BstV1I GCAGC 5 cut(s) 793, 796, 850, 928, 986
BstV2I GAAGAC 2 cut(s) 99, 485
BstZI CGGCCG 1 cut(s) 875
Bsu15I ATCGAT 1 cut(s) 525
BsuI GTATCC 1 cut(s) 138
BsuRI GGCC 1 cut(s) 877
BsuTUI ATCGAT 1 cut(s) 525
BtgZI GCGATG 1 cut(s) 819
BtsCI GGATG 2 cut(s) 280, 642
BtsIMutI CAGTG 1 cut(s) 569
Cac8I GCNNGC 1 cut(s) 618
CfoI GCGC 1 cut(s) 409
Cfr13I GGNCC 2 cut(s) 684, 752
ClaI ATCGAT 1 cut(s) 525
Csp6I GTAC 2 cut(s) 174, 741
CviAII CATG 4 cut(s) 577, 864, 913, 938
CviQI GTAC 2 cut(s) 174, 741
DdeI CTNAG 2 cut(s) 312, 671
DpnI GATC 4 cut(s) 164, 170, 453, 723
DpnII GATC 4 cut(s) 162, 168, 451, 721
DraIII CACNNNGTG 1 cut(s) 597
EaeI YGGCCR 1 cut(s) 875
EagI CGGCCG 1 cut(s) 875
EciI GGCGGA 1 cut(s) 48
EclXI CGGCCG 1 cut(s) 875
Eco24I GRGCYC 1 cut(s) 584
Eco47I GGWCC 2 cut(s) 684, 752
Eco52I CGGCCG 1 cut(s) 875
EcoNI CCTNNNNNAGG 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 684
EcoRI GAATTC 2 cut(s) 292, 352
EcoT38I GRGCYC 1 cut(s) 584
FaeI CATG 4 cut(s) 580, 867, 916, 941
FaqI GGGAC 1 cut(s) 697
FatI CATG 4 cut(s) 576, 863, 912, 937
FauI CCCGC 1 cut(s) 609
FauNDI CATATG 1 cut(s) 487
FbaI TGATCA 1 cut(s) 721
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 8 cut(s) 370, 782, 785, 839, 917, 920, 923, 975
FokI GGATG 2 cut(s) 267, 649
FriOI GRGCYC 1 cut(s) 584
Fsp4HI GCNGC 8 cut(s) 370, 782, 785, 839, 917, 920, 923, 975
FspBI CTAG 2 cut(s) 116, 300
GlaI GCGC 1 cut(s) 408
GluI GCNGC 8 cut(s) 370, 782, 785, 839, 917, 920, 923, 975
HaeIII GGCC 1 cut(s) 877
HapII CCGG 1 cut(s) 979
HhaI GCGC 1 cut(s) 409
Hin1II CATG 4 cut(s) 580, 867, 916, 941
Hin6I GCGC 1 cut(s) 407
HinP1I GCGC 1 cut(s) 407
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 4 cut(s) 15, 185, 491, 562
HpaII CCGG 1 cut(s) 979
HphI GGTGA 4 cut(s) 17, 186, 361, 753
Hpy166II GTNNAC 2 cut(s) 55, 600
Hpy188I TCNGA 5 cut(s) 73, 91, 265, 397, 693
Hpy188III TCNNGA 7 cut(s) 116, 166, 413, 608, 955, 964, 989
Hpy8I GTNNAC 2 cut(s) 55, 600
HpyAV CCTTC 2 cut(s) 627, 915
HpyCH4III ACNGT 1 cut(s) 126
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 7 cut(s) 145, 271, 542, 600, 781, 916, 974
HpyF10VI GCNNNNNNNGC 6 cut(s) 277, 286, 778, 790, 859, 922
HpyF3I CTNAG 2 cut(s) 312, 671
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 4 cut(s) 580, 867, 916, 941
HspAI GCGC 1 cut(s) 407
Ksp22I TGATCA 1 cut(s) 721
Kzo9I GATC 4 cut(s) 162, 168, 451, 721
LmnI GCTCC 1 cut(s) 587
Lsp1109I GCAGC 5 cut(s) 793, 796, 850, 928, 986
LweI GCATC 4 cut(s) 154, 289, 418, 961
MaeI CTAG 2 cut(s) 116, 300
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 1 cut(s) 709
MalI GATC 4 cut(s) 164, 170, 453, 723
MboI GATC 4 cut(s) 162, 168, 451, 721
MboII GAAGA 7 cut(s) 86, 104, 167, 252, 490, 687, 728
MhlI GDGCHC 3 cut(s) 584, 602, 855
MluCI AATT 9 cut(s) 25, 292, 331, 352, 384, 390, 930, 1007, 1069
MlyI GAGTC 2 cut(s) 9, 571
MmeI TCCRAC 2 cut(s) 118, 730
MnlI CCTC 6 cut(s) 242, 297, 300, 307, 307, 804
MseI TTAA 3 cut(s) 24, 330, 1052
MslI CAYNNNNRTG 1 cut(s) 150
MspCI CTTAAG 1 cut(s) 1051
MspI CCGG 1 cut(s) 979
MspR9I CCNGG 1 cut(s) 979
MwoI GCNNNNNNNGC 6 cut(s) 277, 286, 778, 790, 859, 922
NciI CCSGG 1 cut(s) 979
NdeI CATATG 1 cut(s) 487
NdeII GATC 4 cut(s) 162, 168, 451, 721
NlaIII CATG 4 cut(s) 580, 867, 916, 941
NlaIV GGNNCC 3 cut(s) 583, 685, 729
NspI RCATGY 1 cut(s) 916
PfeI GAWTC 2 cut(s) 185, 491
PkrI GCNGC 8 cut(s) 371, 783, 786, 840, 918, 921, 924, 976
PleI GAGTC 2 cut(s) 9, 570
PpsI GAGTC 2 cut(s) 9, 570
PpuMI RGGWCCY 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 684
PspN4I GGNNCC 3 cut(s) 583, 685, 729
PspPI GGNCC 2 cut(s) 684, 752
PspPPI RGGWCCY 1 cut(s) 684
RsaI GTAC 2 cut(s) 175, 742
RsaNI GTAC 2 cut(s) 174, 741
RseI CAYNNNNRTG 1 cut(s) 150
SaqAI TTAA 3 cut(s) 24, 330, 1052
SatI GCNGC 8 cut(s) 370, 782, 785, 839, 917, 920, 923, 975
Sau3AI GATC 4 cut(s) 162, 168, 451, 721
Sau96I GGNCC 2 cut(s) 684, 752
SchI GAGTC 2 cut(s) 9, 571
ScrFI CCNGG 1 cut(s) 979
SduI GDGCHC 3 cut(s) 584, 602, 855
SfaNI GCATC 4 cut(s) 154, 289, 418, 961
SinI GGWCC 2 cut(s) 684, 752
SmiMI CAYNNNNRTG 1 cut(s) 150
SmlI CTYRAG 3 cut(s) 744, 953, 1051
SmoI CTYRAG 3 cut(s) 744, 953, 1051
Sse9I AATT 9 cut(s) 25, 292, 331, 352, 384, 390, 930, 1007, 1069
SsiI CCGC 7 cut(s) 33, 289, 369, 483, 616, 920, 923
SspI AATATT 1 cut(s) 1039
SspMI CTAG 2 cut(s) 116, 300
StyD4I CCNGG 1 cut(s) 977
TaaI ACNGT 1 cut(s) 126
TaiI ACGT 1 cut(s) 366
TaqI TCGA 2 cut(s) 148, 525
TasI AATT 9 cut(s) 25, 292, 331, 352, 384, 390, 930, 1007, 1069
TauI GCSGC 3 cut(s) 372, 922, 925
TfiI GAWTC 2 cut(s) 185, 491
Tru1I TTAA 3 cut(s) 24, 330, 1052
Tru9I TTAA 3 cut(s) 24, 330, 1052
TscAI CASTG 1 cut(s) 576
TseI GCWGC 5 cut(s) 781, 784, 838, 916, 974
TspDTI ATGAA 7 cut(s) 168, 198, 474, 491, 504, 692, 923
TspGWI ACGGA 1 cut(s) 876
TspRI CASTG 1 cut(s) 576
Vha464I CTTAAG 1 cut(s) 1051
VneI GTGCAC 1 cut(s) 598
VpaK11BI GGWCC 2 cut(s) 684, 752
XagI CCTNNNNNAGG 1 cut(s) 301
XapI RAATTY 2 cut(s) 292, 352
XbaI TCTAGA 1 cut(s) 115
XceI RCATGY 1 cut(s) 916
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 2 cut(s) 116, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.