RLG00000033640

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000885
Physical Location & Seq
Reverse (-)
29919445 .. 30083003
163559 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033640

Sequence Viewer

Length: 1092 bp
ATGTCATTCCGGTCAGCTGAACTTCTAATACCGATCGATGGTCTCCATAGAGTCGACGTCTTGTTTCTCGATGCACGGTTTATCGGTCCGGACAGCAGAGGTAGACCACAAAGAGCATTTTCATCCCTTTCTGAGCTTCCCCGAGATATTCCACGTGGAGTCCTTCTGGATAAGTTTGACATAAGGAAGGAACATCTAGCTAAAGTAACTGGATTGCTGGATTCAAATCCGGAATTATCCGTAAGGTTATCTCGACAACCAGAAGCTTATATATATGTTGTCACTGAAAGGTTGGCACAGTCGGAGAGGGCATTGGATGACGTCTCGGAGATTGTAGAAAAAACAGTGCTGCCAATATTCTACGATGTTGATCCCTCCGATGTACGACATCAAACAGGAAGTTTTGCACAGGCCTTTGCCAACTATGAGATAAAGTACAAGGATAACCCACAAAAAGTAAAAAGGTGGAGAGCTGCTTTAACGAAACTGGGAAGTCTCGCAGGCTGGCTGGCATTCAGGGGGCTGTGTTATACTTTACGACGGCACGAAACAAAACTCATTCAAGAAGTTGTTGATGTGACATGGAGAAAATTGTATCCCTCTTTGTCAAAACCTGCCATTAAAAATCTGGCATCAACACCTTCTTGGTCTTCTTCTTATCGACGAAAGCATGATGTCTTTCTAAACTTTAGAGGGGCGGATACCCGCTTGACATTTATGGGCCATCTGCATGCTGCATTAATTCACCGCGGAATTTCCACCTTTAGAGATGACATAGATCTTGAGAAAGGAAAGTCCATTTCCAGACAACTCGACAAAGCAATTGAGGCATCAAAGGTTTGGGTTGTCATAGTTTCACCAGGCTACGCTTCTTCAAAATGGTGCTTGAATGAGCTTTTGAAGATTCTTGAATGCAGAAAAGCATCTAAGGTGGGAAGGAAGAATCACACTTTGATGAAGCCATCTGCAGAATATGAAGAACAAGCTCGTACTCCGGTGAATGGAGACAAAACACATAAGGGGAAGGCTGGTTTGCGGAACAAGGAAAACATCGCTAATCATAATAAAAGGTTATTCATCTTCAGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

364

Amino Acids

41.47

Weight (kDa)

9.81

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 90 - 204 1.1e-13 TIR domain
TIR PF01582 223 - 309 2.4e-27 TIR domain
TIR_2 PF13676 226 - 306 5.4e-13 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 60, 324
Acc36I ACCTGC 1 cut(s) 622
AccI GTMKAC 2 cut(s) 54, 103
AccII CGCG 1 cut(s) 750
AccIII TCCGGA 2 cut(s) 88, 229
AciI CCGC 5 cut(s) 698, 706, 748, 750, 1036
AclWI GGATC 1 cut(s) 365
AcsI RAATTY 1 cut(s) 753
AcuI CTGAAG 1 cut(s) 1066
AcvI CACGTG 1 cut(s) 155
AcyI GRCGYC 2 cut(s) 57, 321
AfaI GTAC 3 cut(s) 384, 437, 991
AfiI CCNNNNNNNGG 2 cut(s) 38, 1001
AgsI TTSAA 6 cut(s) 225, 563, 876, 889, 901, 911
AjnI CCWGG 1 cut(s) 859
AluBI AGCT 7 cut(s) 17, 136, 200, 266, 473, 895, 986
AluI AGCT 7 cut(s) 17, 136, 200, 266, 473, 895, 986
Alw26I GTCTC 4 cut(s) 47, 328, 500, 999
AlwI GGATC 1 cut(s) 365
Ama87I CYCGRG 1 cut(s) 141
Aor13HI TCCGGA 2 cut(s) 88, 229
AoxI GGCC 2 cut(s) 411, 721
ApeKI GCWGC 3 cut(s) 349, 473, 734
ApoI RAATTY 1 cut(s) 753
AseI ATTAAT 1 cut(s) 740
AspS9I GGNCC 2 cut(s) 86, 721
AsuHPI GGTGA 3 cut(s) 737, 849, 1009
AvaI CYCGRG 1 cut(s) 141
AvaII GGWCC 1 cut(s) 86
BbrPI CACGTG 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 642
BbvI GCAGC 3 cut(s) 336, 460, 721
BccI CCATC 3 cut(s) 32, 732, 970
BceAI ACGGC 1 cut(s) 557
BciT130I CCWGG 1 cut(s) 861
BciVI GTATCC 2 cut(s) 606, 694
BcoDI GTCTC 4 cut(s) 47, 328, 500, 999
BfaI CTAG 2 cut(s) 197, 1090
BfmI CTRYAG 1 cut(s) 966
BfuAI ACCTGC 1 cut(s) 622
BfuI GTATCC 2 cut(s) 606, 694
BglII AGATCT 1 cut(s) 778
BisI GCNGC 3 cut(s) 350, 474, 735
BlsI GCNGC 3 cut(s) 351, 475, 736
Bme1390I CCNGG 1 cut(s) 861
Bme18I GGWCC 1 cut(s) 86
BmeT110I CYCGRG 1 cut(s) 141
BmgT120I GGNCC 2 cut(s) 86, 721
BmrFI CCNGG 1 cut(s) 861
BmrI ACTGGG 1 cut(s) 497
BmsI GCATC 4 cut(s) 61, 641, 839, 932
BmuI ACTGGG 1 cut(s) 497
BpiI GAAGAC 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 803
Bsa29I ATCGAT 1 cut(s) 36
BsaAI YACGTR 1 cut(s) 155
BsaBI GATNNNNATC 2 cut(s) 225, 369
BsaHI GRCGYC 2 cut(s) 57, 321
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 1 cut(s) 748
BsaWI WCCGGW 4 cut(s) 9, 88, 229, 994
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 1001
Bse1I ACTGG 2 cut(s) 214, 492
Bse8I GATNNNNATC 2 cut(s) 225, 369
BseAI TCCGGA 2 cut(s) 88, 229
BseBI CCWGG 1 cut(s) 861
BseCI ATCGAT 1 cut(s) 36
BseDI CCNNGG 1 cut(s) 748
BseGI GGATG 2 cut(s) 122, 322
BseJI GATNNNNATC 2 cut(s) 225, 369
BseLI CCNNNNNNNGG 2 cut(s) 38, 1001
BseMII CTCAG 1 cut(s) 123
BseNI ACTGG 2 cut(s) 214, 492
BseXI GCAGC 3 cut(s) 336, 460, 721
Bsh1236I CGCG 1 cut(s) 750
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 2 cut(s) 413, 723
BshVI ATCGAT 1 cut(s) 36
BsiEI CGRYCG 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 141
BsiSI CCGG 4 cut(s) 10, 89, 230, 995
BslI CCNNNNNNNGG 2 cut(s) 38, 1001
BsmAI GTCTC 4 cut(s) 47, 328, 500, 999
BsmBI CGTCTC 1 cut(s) 328
BsmI GAATGC 2 cut(s) 512, 917
BsnI GGCC 2 cut(s) 413, 723
Bso31I GGTCTC 1 cut(s) 47
BsoBI CYCGRG 1 cut(s) 141
Bsp13I TCCGGA 2 cut(s) 88, 229
Bsp143I GATC 3 cut(s) 33, 370, 778
BspACI CCGC 5 cut(s) 698, 706, 748, 750, 1036
BspANI GGCC 2 cut(s) 413, 723
BspCNI CTCAG 1 cut(s) 124
BspDI ATCGAT 1 cut(s) 36
BspEI TCCGGA 2 cut(s) 88, 229
BspFNI CGCG 1 cut(s) 750
BspMAI CTGCAG 1 cut(s) 970
BspMI ACCTGC 1 cut(s) 622
BspPI GGATC 1 cut(s) 365
BspTNI GGTCTC 1 cut(s) 47
BsrI ACTGG 2 cut(s) 214, 492
BssECI CCNNGG 1 cut(s) 748
BssMI GATC 3 cut(s) 33, 370, 778
BssNI GRCGYC 2 cut(s) 57, 321
Bst2UI CCWGG 1 cut(s) 861
Bst4CI ACNGT 3 cut(s) 78, 300, 346
BstACI GRCGYC 2 cut(s) 57, 321
BstBAI YACGTR 1 cut(s) 155
BstC8I GCNNGC 4 cut(s) 502, 506, 510, 732
BstDEI CTNAG 2 cut(s) 132, 927
BstDSI CCRYGG 1 cut(s) 748
BstF5I GGATG 2 cut(s) 122, 322
BstFNI CGCG 1 cut(s) 750
BstKTI GATC 3 cut(s) 36, 373, 781
BstMAI GTCTC 4 cut(s) 47, 328, 500, 999
BstMBI GATC 3 cut(s) 33, 370, 778
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 1 cut(s) 827
BstNI CCWGG 1 cut(s) 861
BstNSI RCATGY 1 cut(s) 734
BstSCI CCNGG 1 cut(s) 859
BstSFI CTRYAG 1 cut(s) 966
BstUI CGCG 1 cut(s) 750
BstV1I GCAGC 3 cut(s) 336, 460, 721
BstV2I GAAGAC 1 cut(s) 642
BstX2I RGATCY 1 cut(s) 778
BstYI RGATCY 1 cut(s) 778
Bsu15I ATCGAT 1 cut(s) 36
BsuI GTATCC 2 cut(s) 606, 694
BsuRI GGCC 2 cut(s) 413, 723
BsuTUI ATCGAT 1 cut(s) 36
BtgI CCRYGG 1 cut(s) 748
BtgZI GCGATG 1 cut(s) 1036
BtsCI GGATG 2 cut(s) 122, 322
BtsIMutI CAGTG 2 cut(s) 282, 351
BveI ACCTGC 1 cut(s) 622
Cac8I GCNNGC 4 cut(s) 502, 506, 510, 732
Cfr13I GGNCC 2 cut(s) 86, 721
Cfr42I CCGCGG 1 cut(s) 751
ClaI ATCGAT 1 cut(s) 36
CpoI CGGWCCG 1 cut(s) 86
Csp6I GTAC 3 cut(s) 383, 436, 990
CspI CGGWCCG 1 cut(s) 86
CviAII CATG 3 cut(s) 582, 671, 731
CviQI GTAC 3 cut(s) 383, 436, 990
DdeI CTNAG 2 cut(s) 132, 927
DpnI GATC 3 cut(s) 35, 372, 780
DpnII GATC 3 cut(s) 33, 370, 778
EciI GGCGGA 1 cut(s) 713
Eco147I AGGCCT 1 cut(s) 413
Eco31I GGTCTC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 86
Eco57I CTGAAG 1 cut(s) 1066
Eco72I CACGTG 1 cut(s) 155
Eco88I CYCGRG 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 859
Esp3I CGTCTC 1 cut(s) 328
FaeI CATG 3 cut(s) 585, 674, 734
FatI CATG 3 cut(s) 581, 670, 730
FauI CCCGC 1 cut(s) 713
FblI GTMKAC 2 cut(s) 54, 103
Fnu4HI GCNGC 3 cut(s) 350, 474, 735
FokI GGATG 2 cut(s) 109, 329
Fsp4HI GCNGC 3 cut(s) 350, 474, 735
FspBI CTAG 2 cut(s) 197, 1090
GluI GCNGC 3 cut(s) 350, 474, 735
HaeIII GGCC 2 cut(s) 413, 723
HapII CCGG 4 cut(s) 10, 89, 230, 995
Hin1I GRCGYC 2 cut(s) 57, 321
Hin1II CATG 3 cut(s) 585, 674, 734
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 5 cut(s) 51, 159, 221, 904, 943
HpaII CCGG 4 cut(s) 10, 89, 230, 995
HphI GGTGA 3 cut(s) 737, 849, 1009
Hpy166II GTNNAC 2 cut(s) 55, 104
Hpy188I TCNGA 4 cut(s) 133, 304, 328, 379
Hpy188III TCNNGA 9 cut(s) 68, 89, 167, 230, 252, 563, 782, 804, 908
Hpy8I GTNNAC 2 cut(s) 55, 104
Hpy99I CGWCG 3 cut(s) 59, 543, 666
HpyAV CCTTC 5 cut(s) 173, 181, 651, 930, 1018
HpyCH4III ACNGT 3 cut(s) 78, 300, 346
HpyCH4IV ACGT 3 cut(s) 57, 154, 321
HpyCH4V TGCA 6 cut(s) 74, 407, 730, 737, 915, 968
HpyF10VI GCNNNNNNNGC 1 cut(s) 827
HpyF3I CTNAG 2 cut(s) 132, 927
HpySE526I ACGT 3 cut(s) 57, 154, 321
Hsp92I GRCGYC 2 cut(s) 57, 321
Hsp92II CATG 3 cut(s) 585, 674, 734
Kpn2I TCCGGA 2 cut(s) 88, 229
KspI CCGCGG 1 cut(s) 751
Kzo9I GATC 3 cut(s) 33, 370, 778
Lsp1109I GCAGC 3 cut(s) 336, 460, 721
LweI GCATC 4 cut(s) 61, 641, 839, 932
MaeI CTAG 2 cut(s) 197, 1090
MaeII ACGT 3 cut(s) 57, 154, 321
MaeIII GTNAC 3 cut(s) 205, 280, 577
MalI GATC 3 cut(s) 35, 372, 780
MboI GATC 3 cut(s) 33, 370, 778
MboII GAAGA 7 cut(s) 642, 645, 864, 913, 952, 989, 1072
MfeI CAATTG 1 cut(s) 822
MflI RGATCY 1 cut(s) 778
MluCI AATT 5 cut(s) 233, 590, 741, 753, 822
MlyI GAGTC 2 cut(s) 60, 168
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 6 cut(s) 92, 300, 385, 610, 686, 820
MroI TCCGGA 2 cut(s) 88, 229
MseI TTAA 3 cut(s) 479, 621, 740
MslI CAYNNNNRTG 2 cut(s) 729, 953
MspA1I CMGCKG 2 cut(s) 17, 750
MspI CCGG 4 cut(s) 10, 89, 230, 995
MspR9I CCNGG 1 cut(s) 861
MunI CAATTG 1 cut(s) 822
Mva1269I GAATGC 2 cut(s) 512, 917
MvaI CCWGG 1 cut(s) 861
MvnI CGCG 1 cut(s) 750
MwoI GCNNNNNNNGC 1 cut(s) 827
NdeII GATC 3 cut(s) 33, 370, 778
NlaIII CATG 3 cut(s) 585, 674, 734
NmuCI GTSAC 2 cut(s) 280, 577
NspI RCATGY 1 cut(s) 734
PaeI GCATGC 1 cut(s) 734
PceI AGGCCT 1 cut(s) 413
PctI GAATGC 2 cut(s) 512, 917
PfeI GAWTC 3 cut(s) 221, 904, 943
PkrI GCNGC 3 cut(s) 351, 475, 736
Ple19I CGATCG 1 cut(s) 36
PleI GAGTC 2 cut(s) 59, 167
PmaCI CACGTG 1 cut(s) 155
PmlI CACGTG 1 cut(s) 155
PpsI GAGTC 2 cut(s) 59, 167
Ppu21I YACGTR 1 cut(s) 155
PshBI ATTAAT 1 cut(s) 740
Psp6I CCWGG 1 cut(s) 859
PspCI CACGTG 1 cut(s) 155
PspGI CCWGG 1 cut(s) 859
PspPI GGNCC 2 cut(s) 86, 721
PsrI GAACNNNNNNTAC 2 cut(s) 12, 44
PstI CTGCAG 1 cut(s) 970
PsuI RGATCY 1 cut(s) 778
PvuI CGATCG 1 cut(s) 36
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 3 cut(s) 384, 437, 991
RsaNI GTAC 3 cut(s) 383, 436, 990
RseI CAYNNNNRTG 2 cut(s) 729, 953
Rsr2I CGGWCCG 1 cut(s) 86
RsrII CGGWCCG 1 cut(s) 86
SacII CCGCGG 1 cut(s) 751
SalI GTCGAC 1 cut(s) 53
SaqAI TTAA 3 cut(s) 479, 621, 740
SatI GCNGC 3 cut(s) 350, 474, 735
Sau3AI GATC 3 cut(s) 33, 370, 778
Sau96I GGNCC 2 cut(s) 86, 721
SchI GAGTC 2 cut(s) 60, 168
ScrFI CCNGG 1 cut(s) 861
SfaNI GCATC 4 cut(s) 61, 641, 839, 932
SfcI CTRYAG 1 cut(s) 966
Sfr303I CCGCGG 1 cut(s) 751
SgrBI CCGCGG 1 cut(s) 751
SinI GGWCC 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 729, 953
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
SphI GCATGC 1 cut(s) 734
Sse9I AATT 5 cut(s) 233, 590, 741, 753, 822
SseBI AGGCCT 1 cut(s) 413
SsiI CCGC 5 cut(s) 698, 706, 748, 750, 1036
SspI AATATT 1 cut(s) 357
SspMI CTAG 2 cut(s) 197, 1090
StuI AGGCCT 1 cut(s) 413
StyD4I CCNGG 1 cut(s) 859
TaaI ACNGT 3 cut(s) 78, 300, 346
TaiI ACGT 3 cut(s) 60, 157, 324
TaqI TCGA 6 cut(s) 36, 54, 69, 253, 661, 813
TaqII GACCGA 1 cut(s) 74
TasI AATT 5 cut(s) 233, 590, 741, 753, 822
TatI WGTACW 1 cut(s) 435
TfiI GAWTC 3 cut(s) 221, 904, 943
Tru1I TTAA 3 cut(s) 479, 621, 740
Tru9I TTAA 3 cut(s) 479, 621, 740
TscAI CASTG 2 cut(s) 289, 351
TseFI GTSAC 2 cut(s) 280, 577
TseI GCWGC 3 cut(s) 349, 473, 734
Tsp45I GTSAC 2 cut(s) 280, 577
TspDTI ATGAA 4 cut(s) 111, 971, 990, 1066
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 2 cut(s) 289, 351
VpaK11BI GGWCC 1 cut(s) 86
VspI ATTAAT 1 cut(s) 740
XapI RAATTY 1 cut(s) 753
XceI RCATGY 1 cut(s) 734
XcmI CCANNNNNNNNNTGG 1 cut(s) 625
XmiI GTMKAC 2 cut(s) 54, 103
XspI CTAG 2 cut(s) 197, 1090
ZraI GACGTC 2 cut(s) 58, 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.