RLG00000033630

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000885
Physical Location & Seq
Reverse (-)
29787099 .. 29789027
1929 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033630

Sequence Viewer

Length: 582 bp
ATGTTGATCCCTCCGATGTACGACATCAAACAGGAAGCTGGCTGGCATTCAGGGGGCTGGCACGAAACAAAACTCATTCAAGAAGTTGTTGATGTGACATGGAGAAAATTGTATCCCTCTTTGTCAAAACCTGCCATTAAAAATCTGGCATCAACACCTTCTTGGTCTTCTTCTTATCGACGAAAGCATGATGTCTTTCTAAACTTTAAAGGGGCGGATACCCGCTTGACATTTATGGGCCATCTGCATGCTGCATTAATTCACCGCGGAATTTCCACCTTTAGAGATGACATAGATCTTGAGAAAGGAAAGTCCATTTCCAGACGACTCGACAAAGCAATTGAGGCATCAAAGGTTTGGGTTGTCATCGTTTCACCAGGCTATGCTTCTTCAAAATGGTGCTTGAATGAGCTTTTGAAGATTCTTGAATGCAGAAAAGCATCTAAGGTGGGAAGGAAGAATCAAACTTTGATGAAGCCATCTGCAGAATATGAAGAACAAGCTCGTACTCCGGTGAATGGAGACAAAACACATAAGGGGAAGGCTGGTTTGCGGGAACAAGGAAAACATCGCTTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

194

Amino Acids

21.95

Weight (kDa)

9.87

Isoelectric Point (pI)

35.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 62 - 148 2.2e-27 TIR domain
TIR_2 PF13676 65 - 145 2.1e-13 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 139
AccII CGCG 1 cut(s) 267
AciI CCGC 5 cut(s) 215, 223, 265, 267, 553
AcsI RAATTY 1 cut(s) 270
AfaI GTAC 2 cut(s) 20, 508
AfiI CCNNNNNNNGG 1 cut(s) 518
AgsI TTSAA 5 cut(s) 80, 393, 406, 418, 428
AjnI CCWGG 1 cut(s) 376
AluBI AGCT 3 cut(s) 38, 412, 503
AluI AGCT 3 cut(s) 38, 412, 503
Alw26I GTCTC 1 cut(s) 516
AoxI GGCC 1 cut(s) 238
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 1 cut(s) 270
AseI ATTAAT 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 238
AsuHPI GGTGA 3 cut(s) 254, 366, 526
BbsI GAAGAC 1 cut(s) 159
BbvI GCAGC 1 cut(s) 238
BccI CCATC 2 cut(s) 249, 487
BciT130I CCWGG 1 cut(s) 378
BciVI GTATCC 2 cut(s) 123, 211
BcoDI GTCTC 1 cut(s) 516
BfmI CTRYAG 1 cut(s) 483
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 2 cut(s) 123, 211
BglII AGATCT 1 cut(s) 295
BisI GCNGC 1 cut(s) 252
BlsI GCNGC 1 cut(s) 253
Bme1390I CCNGG 1 cut(s) 378
BmgT120I GGNCC 1 cut(s) 238
BmrFI CCNGG 1 cut(s) 378
BmsI GCATC 3 cut(s) 158, 356, 449
BpiI GAAGAC 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 320
BsaJI CCNNGG 1 cut(s) 265
BsaWI WCCGGW 1 cut(s) 511
Bsc4I CCNNNNNNNGG 1 cut(s) 518
BseBI CCWGG 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 265
BseLI CCNNNNNNNGG 1 cut(s) 518
BseXI GCAGC 1 cut(s) 238
Bsh1236I CGCG 1 cut(s) 267
BshFI GGCC 1 cut(s) 240
BsiSI CCGG 1 cut(s) 512
BslI CCNNNNNNNGG 1 cut(s) 518
BsmAI GTCTC 1 cut(s) 516
BsmI GAATGC 2 cut(s) 46, 434
BsnI GGCC 1 cut(s) 240
Bsp143I GATC 2 cut(s) 6, 295
BspACI CCGC 5 cut(s) 215, 223, 265, 267, 553
BspANI GGCC 1 cut(s) 240
BspFNI CGCG 1 cut(s) 267
BspMAI CTGCAG 1 cut(s) 487
BspMI ACCTGC 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 2 cut(s) 6, 295
Bst2UI CCWGG 1 cut(s) 378
BstC8I GCNNGC 4 cut(s) 40, 44, 59, 249
BstDEI CTNAG 1 cut(s) 444
BstDSI CCRYGG 1 cut(s) 265
BstFNI CGCG 1 cut(s) 267
BstKTI GATC 2 cut(s) 9, 298
BstMAI GTCTC 1 cut(s) 516
BstMBI GATC 2 cut(s) 6, 295
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNI CCWGG 1 cut(s) 378
BstNSI RCATGY 1 cut(s) 251
BstSCI CCNGG 1 cut(s) 376
BstSFI CTRYAG 1 cut(s) 483
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 238
BstV2I GAAGAC 1 cut(s) 159
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BsuI GTATCC 2 cut(s) 123, 211
BsuRI GGCC 1 cut(s) 240
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 554
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 4 cut(s) 40, 44, 59, 249
Cfr13I GGNCC 1 cut(s) 238
Cfr42I CCGCGG 1 cut(s) 268
Csp6I GTAC 2 cut(s) 19, 507
CviAII CATG 3 cut(s) 99, 188, 248
CviJI RGCY 9 cut(s) 38, 42, 57, 240, 381, 412, 478, 503, 545
CviKI_1 RGCY 9 cut(s) 38, 42, 57, 240, 381, 412, 478, 503, 545
CviQI GTAC 2 cut(s) 19, 507
DdeI CTNAG 1 cut(s) 444
DpnI GATC 2 cut(s) 8, 297
DpnII GATC 2 cut(s) 6, 295
DraI TTTAAA 1 cut(s) 208
EciI GGCGGA 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 376
FaeI CATG 3 cut(s) 102, 191, 251
FaiI YATR 9 cut(s) 100, 189, 236, 249, 293, 384, 492, 534, 580
FatI CATG 3 cut(s) 98, 187, 247
FauI CCCGC 2 cut(s) 230, 546
Fnu4HI GCNGC 1 cut(s) 252
Fsp4HI GCNGC 1 cut(s) 252
GluI GCNGC 1 cut(s) 252
HaeIII GGCC 1 cut(s) 240
HapII CCGG 1 cut(s) 512
Hin1II CATG 3 cut(s) 102, 191, 251
HinfI GANTC 3 cut(s) 327, 421, 460
HpaII CCGG 1 cut(s) 512
HphI GGTGA 3 cut(s) 254, 366, 526
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 4 cut(s) 80, 299, 321, 425
Hpy99I CGWCG 1 cut(s) 183
HpyAV CCTTC 3 cut(s) 168, 447, 535
HpyCH4V TGCA 4 cut(s) 247, 254, 432, 485
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 1 cut(s) 444
Hsp92II CATG 3 cut(s) 102, 191, 251
KspI CCGCGG 1 cut(s) 268
Kzo9I GATC 2 cut(s) 6, 295
Lsp1109I GCAGC 1 cut(s) 238
LweI GCATC 3 cut(s) 158, 356, 449
MaeIII GTNAC 1 cut(s) 94
MalI GATC 2 cut(s) 8, 297
MboI GATC 2 cut(s) 6, 295
MboII GAAGA 6 cut(s) 159, 162, 381, 430, 469, 506
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 1 cut(s) 295
MluCI AATT 4 cut(s) 107, 258, 270, 339
MlyI GAGTC 1 cut(s) 321
MnlI CCTC 3 cut(s) 21, 127, 337
MseI TTAA 3 cut(s) 138, 207, 257
MslI CAYNNNNRTG 1 cut(s) 246
MspA1I CMGCKG 1 cut(s) 267
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 1 cut(s) 378
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 2 cut(s) 46, 434
MvaI CCWGG 1 cut(s) 378
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 1 cut(s) 344
NdeII GATC 2 cut(s) 6, 295
NlaIII CATG 3 cut(s) 102, 191, 251
NmuCI GTSAC 1 cut(s) 94
NspI RCATGY 1 cut(s) 251
PaeI GCATGC 1 cut(s) 251
PctI GAATGC 2 cut(s) 46, 434
PfeI GAWTC 2 cut(s) 421, 460
PkrI GCNGC 1 cut(s) 253
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
PshBI ATTAAT 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PspPI GGNCC 1 cut(s) 238
PstI CTGCAG 1 cut(s) 487
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 2 cut(s) 20, 508
RsaNI GTAC 2 cut(s) 19, 507
RseI CAYNNNNRTG 1 cut(s) 246
SacII CCGCGG 1 cut(s) 268
SaqAI TTAA 3 cut(s) 138, 207, 257
SatI GCNGC 1 cut(s) 252
Sau3AI GATC 2 cut(s) 6, 295
Sau96I GGNCC 1 cut(s) 238
SchI GAGTC 1 cut(s) 321
ScrFI CCNGG 1 cut(s) 378
SetI ASST 8 cut(s) 40, 133, 160, 281, 357, 414, 450, 505
SfaNI GCATC 3 cut(s) 158, 356, 449
SfcI CTRYAG 1 cut(s) 483
Sfr303I CCGCGG 1 cut(s) 268
SgrBI CCGCGG 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
SphI GCATGC 1 cut(s) 251
Sse9I AATT 4 cut(s) 107, 258, 270, 339
SsiI CCGC 5 cut(s) 215, 223, 265, 267, 553
StyD4I CCNGG 1 cut(s) 376
TaqI TCGA 2 cut(s) 178, 330
TasI AATT 4 cut(s) 107, 258, 270, 339
TfiI GAWTC 2 cut(s) 421, 460
Tru1I TTAA 3 cut(s) 138, 207, 257
Tru9I TTAA 3 cut(s) 138, 207, 257
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 2 cut(s) 488, 507
VspI ATTAAT 1 cut(s) 257
XapI RAATTY 1 cut(s) 270
XceI RCATGY 1 cut(s) 251
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.