Rmu_sc0002482.1_g000010

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002482.1
OG0000885
Physical Location & Seq
Forward (+)
42306 .. 42995
690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002482.1_g000010.1.cds

Sequence Viewer

Length: 690 bp
atgttgccatttgcttatatattaacatgtagctcttgcagcaaatcatctgaaattattttctgtgctgagtttcggcgattccggctgcctaaactttcaatgacgtccgatcatctatggaccgaactccgtgccatccatgacgaattgaaggaattgcggtttctgcgactccggcggcctgcactttcagtgacgtctgatgatctatccacaaaactccgtgctgtaggggacagatggaataaattccggaaatcctaccacaatgaaatagggaccatcctcatcctggactggaaatacgtgtttagggtagaacttctatatggaatcgttctggctcttgtcttagctgagaatattcattgcgtttccgaaagaaaacgtccacagatcgagttattcgtgactgagatacttggagtctactccagagctctagaatatcttcacactggaagtttggcacggtcggataataatgtagtagaaaaaacagttctgccaatattctacggtgttgatccctccaacgtacgacatcaaacaggaagttttgcagaagcctttgcagaccatgataaaaagtacaaggaagacccagaaaaagtaaaaaggtggagggatgctttaacgaaactggcaaatctcgctggctgggattcaaagcactggtatgaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

229

Amino Acids

26.77

Weight (kDa)

8.96

Isoelectric Point (pI)

54.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 110, 203
AccI GTMKAC 1 cut(s) 432
AccIII TCCGGA 1 cut(s) 255
AciI CCGC 2 cut(s) 163, 181
AclWI GGATC 1 cut(s) 524
AcsI RAATTY 1 cut(s) 251
AcyI GRCGYC 2 cut(s) 107, 200
AfaI GTAC 2 cut(s) 543, 596
AfiI CCNNNNNNNGG 1 cut(s) 295
AflIII ACRYGT 2 cut(s) 26, 309
AgsI TTSAA 3 cut(s) 102, 154, 672
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 3 cut(s) 33, 359, 443
AluI AGCT 3 cut(s) 33, 359, 443
Alw21I GWGCWC 1 cut(s) 445
AlwI GGATC 1 cut(s) 524
Aor13HI TCCGGA 1 cut(s) 255
AoxI GGCC 1 cut(s) 182
ApeKI GCWGC 2 cut(s) 39, 88
ApoI RAATTY 1 cut(s) 251
Asp700I GAANNNNTTC 2 cut(s) 251, 453
AspS9I GGNCC 2 cut(s) 123, 282
AvaII GGWCC 2 cut(s) 123, 282
BanII GRGCYC 1 cut(s) 445
BbsI GAAGAC 1 cut(s) 609
Bbv12I GWGCWC 1 cut(s) 445
BbvI GCAGC 2 cut(s) 51, 75
BccI CCATC 3 cut(s) 146, 237, 293
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BciT130I CCWGG 1 cut(s) 296
BfaI CTAG 1 cut(s) 446
BfmI CTRYAG 1 cut(s) 231
BisI GCNGC 3 cut(s) 40, 89, 182
BlsI GCNGC 3 cut(s) 41, 90, 183
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 2 cut(s) 123, 282
BmgT120I GGNCC 2 cut(s) 123, 282
BmiI GGNNCC 1 cut(s) 283
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 1 cut(s) 622
BpiI GAAGAC 1 cut(s) 609
BpmI CTGGAG 1 cut(s) 421
BsaAI YACGTR 1 cut(s) 310
BsaHI GRCGYC 2 cut(s) 107, 200
BsaWI WCCGGW 1 cut(s) 255
Bsc4I CCNNNNNNNGG 1 cut(s) 295
Bse1I ACTGG 4 cut(s) 305, 466, 651, 683
Bse3DI GCAATG 1 cut(s) 370
BseAI TCCGGA 1 cut(s) 255
BseBI CCWGG 1 cut(s) 296
BseGI GGATG 4 cut(s) 138, 285, 291, 637
BseLI CCNNNNNNNGG 1 cut(s) 295
BseMI GCAATG 1 cut(s) 370
BseMII CTCAG 3 cut(s) 60, 351, 408
BseNI ACTGG 4 cut(s) 305, 466, 651, 683
BseXI GCAGC 2 cut(s) 51, 75
BseYI CCCAGC 1 cut(s) 663
BsgI GTGCAG 1 cut(s) 171
Bsh1285I CGRYCG 1 cut(s) 479
BshFI GGCC 1 cut(s) 184
BsiEI CGRYCG 1 cut(s) 479
BsiHKAI GWGCWC 1 cut(s) 445
BsiSI CCGG 3 cut(s) 85, 178, 256
BsiWI CGTACG 1 cut(s) 541
BslFI GGGAC 2 cut(s) 251, 295
BslI CCNNNNNNNGG 1 cut(s) 295
BsmFI GGGAC 2 cut(s) 251, 295
BsnI GGCC 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 445
Bsp13I TCCGGA 1 cut(s) 255
Bsp143I GATC 4 cut(s) 112, 208, 399, 529
BspACI CCGC 2 cut(s) 163, 181
BspANI GGCC 1 cut(s) 184
BspCNI CTCAG 3 cut(s) 61, 352, 409
BspEI TCCGGA 1 cut(s) 255
BspLI GGNNCC 1 cut(s) 283
BspPI GGATC 1 cut(s) 524
BsrDI GCAATG 1 cut(s) 370
BsrI ACTGG 4 cut(s) 305, 466, 651, 683
BssMI GATC 4 cut(s) 112, 208, 399, 529
BssNI GRCGYC 2 cut(s) 107, 200
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 3 cut(s) 477, 505, 524
BstACI GRCGYC 2 cut(s) 107, 200
BstBAI YACGTR 1 cut(s) 310
BstC8I GCNNGC 2 cut(s) 186, 661
BstDEI CTNAG 4 cut(s) 69, 355, 360, 417
BstF5I GGATG 4 cut(s) 138, 285, 291, 637
BstKTI GATC 4 cut(s) 115, 211, 402, 532
BstMBI GATC 4 cut(s) 112, 208, 399, 529
BstMCI CGRYCG 1 cut(s) 479
BstMWI GCNNNNNNNGC 5 cut(s) 39, 85, 169, 178, 656
BstNI CCWGG 1 cut(s) 296
BstNSI RCATGY 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 51, 75
BstV2I GAAGAC 1 cut(s) 609
BsuRI GGCC 1 cut(s) 184
BtsCI GGATG 4 cut(s) 138, 285, 291, 637
BtsIMutI CAGTG 3 cut(s) 201, 459, 676
Cac8I GCNNGC 2 cut(s) 186, 661
Cfr13I GGNCC 2 cut(s) 123, 282
Csp6I GTAC 2 cut(s) 542, 595
CviAII CATG 3 cut(s) 27, 143, 584
CviJI RGCY 8 cut(s) 33, 88, 184, 347, 359, 443, 572, 663
CviKI_1 RGCY 8 cut(s) 33, 88, 184, 347, 359, 443, 572, 663
CviQI GTAC 2 cut(s) 542, 595
DdeI CTNAG 4 cut(s) 69, 355, 360, 417
DpnI GATC 4 cut(s) 114, 210, 401, 531
DpnII GATC 4 cut(s) 112, 208, 399, 529
Ecl136II GAGCTC 1 cut(s) 443
Eco24I GRGCYC 1 cut(s) 445
Eco47I GGWCC 2 cut(s) 123, 282
Eco53kI GAGCTC 1 cut(s) 443
EcoICRI GAGCTC 1 cut(s) 443
EcoRII CCWGG 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 445
FaeI CATG 3 cut(s) 30, 146, 587
FaiI YATR 9 cut(s) 18, 20, 28, 121, 144, 331, 333, 585, 684
FaqI GGGAC 2 cut(s) 251, 295
FatI CATG 3 cut(s) 26, 142, 583
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 3 cut(s) 40, 89, 182
FokI GGATG 4 cut(s) 125, 272, 278, 644
FriOI GRGCYC 1 cut(s) 445
Fsp4HI GCNGC 3 cut(s) 40, 89, 182
FspBI CTAG 1 cut(s) 446
GluI GCNGC 3 cut(s) 40, 89, 182
GsaI CCCAGC 1 cut(s) 667
GsuI CTGGAG 1 cut(s) 421
HaeIII GGCC 1 cut(s) 184
HapII CCGG 3 cut(s) 85, 178, 256
Hin1I GRCGYC 2 cut(s) 107, 200
Hin1II CATG 3 cut(s) 30, 146, 587
HinfI GANTC 5 cut(s) 81, 174, 336, 429, 668
HpaII CCGG 3 cut(s) 85, 178, 256
Hpy166II GTNNAC 2 cut(s) 395, 433
Hpy188I TCNGA 5 cut(s) 52, 112, 205, 382, 481
Hpy188III TCNNGA 4 cut(s) 256, 412, 438, 446
Hpy8I GTNNAC 2 cut(s) 395, 433
HpyAV CCTTC 1 cut(s) 148
HpyCH4III ACNGT 3 cut(s) 477, 505, 524
HpyCH4IV ACGT 5 cut(s) 107, 200, 309, 391, 540
HpyCH4V TGCA 4 cut(s) 39, 188, 566, 578
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 85, 169, 178, 656
HpyF3I CTNAG 4 cut(s) 69, 355, 360, 417
HpySE526I ACGT 5 cut(s) 107, 200, 309, 391, 540
Hsp92I GRCGYC 2 cut(s) 107, 200
Hsp92II CATG 3 cut(s) 30, 146, 587
Kpn2I TCCGGA 1 cut(s) 255
Kzo9I GATC 4 cut(s) 112, 208, 399, 529
Lsp1109I GCAGC 2 cut(s) 51, 75
LweI GCATC 1 cut(s) 622
MaeI CTAG 1 cut(s) 446
MaeII ACGT 5 cut(s) 107, 200, 309, 391, 540
MaeIII GTNAC 2 cut(s) 196, 412
MalI GATC 4 cut(s) 114, 210, 401, 531
MboI GATC 4 cut(s) 112, 208, 399, 529
MboII GAAGA 2 cut(s) 446, 614
MhlI GDGCHC 1 cut(s) 445
MluCI AATT 4 cut(s) 54, 149, 158, 251
MlyI GAGTC 2 cut(s) 168, 438
MmeI TCCRAC 2 cut(s) 459, 561
MnlI CCTC 3 cut(s) 299, 544, 621
MroI TCCGGA 1 cut(s) 255
MroXI GAANNNNTTC 2 cut(s) 251, 453
MseI TTAA 2 cut(s) 23, 638
MslI CAYNNNNRTG 1 cut(s) 681
MspI CCGG 3 cut(s) 85, 178, 256
MspR9I CCNGG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 5 cut(s) 39, 85, 169, 178, 656
NdeII GATC 4 cut(s) 112, 208, 399, 529
NlaIII CATG 3 cut(s) 30, 146, 587
NlaIV GGNNCC 1 cut(s) 283
NmuCI GTSAC 2 cut(s) 196, 412
NspI RCATGY 1 cut(s) 30
PciI ACATGT 1 cut(s) 26
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PdmI GAANNNNTTC 2 cut(s) 251, 453
PfeI GAWTC 3 cut(s) 81, 336, 668
Pfl23II CGTACG 1 cut(s) 541
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 3 cut(s) 41, 90, 183
PleI GAGTC 2 cut(s) 168, 437
PpsI GAGTC 2 cut(s) 168, 437
Ppu21I YACGTR 1 cut(s) 310
PscI ACATGT 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 445
Psp6I CCWGG 1 cut(s) 294
PspFI CCCAGC 1 cut(s) 663
PspGI CCWGG 1 cut(s) 294
PspLI CGTACG 1 cut(s) 541
PspN4I GGNNCC 1 cut(s) 283
PspPI GGNCC 2 cut(s) 123, 282
RsaI GTAC 2 cut(s) 543, 596
RsaNI GTAC 2 cut(s) 542, 595
RseI CAYNNNNRTG 1 cut(s) 681
SacI GAGCTC 1 cut(s) 445
SaqAI TTAA 2 cut(s) 23, 638
SatI GCNGC 3 cut(s) 40, 89, 182
Sau3AI GATC 4 cut(s) 112, 208, 399, 529
Sau96I GGNCC 2 cut(s) 123, 282
SchI GAGTC 2 cut(s) 168, 438
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 1 cut(s) 445
SetI ASST 9 cut(s) 35, 110, 203, 312, 361, 394, 445, 543, 626
SfaNI GCATC 1 cut(s) 622
SfcI CTRYAG 1 cut(s) 231
SinI GGWCC 2 cut(s) 123, 282
SmiMI CAYNNNNRTG 1 cut(s) 681
Sse9I AATT 4 cut(s) 54, 149, 158, 251
SsiI CCGC 2 cut(s) 163, 181
SspI AATATT 2 cut(s) 367, 516
SspMI CTAG 1 cut(s) 446
SstI GAGCTC 1 cut(s) 445
StyD4I CCNGG 1 cut(s) 294
TaaI ACNGT 3 cut(s) 477, 505, 524
TaiI ACGT 5 cut(s) 110, 203, 312, 394, 543
TaqI TCGA 1 cut(s) 402
TaqII GACCGA 1 cut(s) 140
TasI AATT 4 cut(s) 54, 149, 158, 251
TatI WGTACW 1 cut(s) 594
TauI GCSGC 1 cut(s) 184
TfiI GAWTC 3 cut(s) 81, 336, 668
Tru1I TTAA 2 cut(s) 23, 638
Tru9I TTAA 2 cut(s) 23, 638
TscAI CASTG 3 cut(s) 201, 466, 683
TseFI GTSAC 2 cut(s) 196, 412
TseI GCWGC 2 cut(s) 39, 88
Tsp45I GTSAC 2 cut(s) 196, 412
TspDTI ATGAA 2 cut(s) 288, 359
TspGWI ACGGA 2 cut(s) 122, 215
TspRI CASTG 3 cut(s) 201, 466, 683
VpaK11BI GGWCC 2 cut(s) 123, 282
XapI RAATTY 1 cut(s) 251
XbaI TCTAGA 1 cut(s) 445
XceI RCATGY 1 cut(s) 30
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XmiI GTMKAC 1 cut(s) 432
XmnI GAANNNNTTC 2 cut(s) 251, 453
XspI CTAG 1 cut(s) 446
ZraI GACGTC 2 cut(s) 108, 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.