MD03G1083600.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000885
Physical Location & Seq
Reverse (-)
6767857 .. 6771947
4091 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1083600.v1.1.491

Sequence Viewer

Length: 1599 bp
ATGGCATCAAGCACTTTGGGTTTGAATTCATCTCCTCCAGATGATAGGTTGAAATATGATGTATTTTTGAGTTTTCGAGGAGAAGACACGAGAAAGGGTTTTGTAAGTCATCTATATGAAGCGCTTGAGAACGCCGGCGTAAAAACTTTCCACGATGATGCTCCAAGAGATATTAGCCCAGAACTCAAGGCTGCAATTCAGAATTCGAGGACTGCAATTACTTTTCTGGGAGAGAATTTTGCATCGTCGCCATGGGCATTGGAGGAGCTTGACCTGATTCTTAAATCCCATGGAAAAAGAAAAACGGTGCTACCAATTTTCTATAATGTCGATCCCTCTGACGTACGATATCAAACAGGGAGTTTTGCTGAAGCCTTTGCTAGACACGAAGAAACATATAAGGATGACCCAGACAAGGTTCGAAGGTGGAGAGCTGCTTTAACCCAAGTAGCAAACCTCACTGGGTGGGTTTCAAGGGGCCGGGACGAATCAGAGCTCATTGAAGAAATTGTTGATGTAATACGGAAGCTTTTGCACCCTACGACTAAAACCTCAGCACCTTGTTCTTCACTTTTTTCTTCTCACAAACGGAAGTATGATGTGTTTATGTGTTTTAGAGGCAAAGACACCCGCTTGAATTTTGTGGGTCATACGTACGCTTCACTGAATCGGGAAAAATTTACCATCTTTAAAGATGACAAAGGGCTTGAGAAAGGACAACCAATTTCCCCAGGAATTGTAAAAGCAATTGAGGAATCACAGATTTACCTCGTCACTCTTTCACCAGATTTTGCTTCTTCAACTTGGTGCTTGGATGAGCTTGTGAAGATGTTTGAATGCATGGAAACATCCAACTCACAGAGGAGTCTCGAGGACTTTACAGAAAGAAAGGATGCATCGACAGTCGCGACCGGTATCAAGTGTAACACAAGGGATAGGAAAATAAGTAAAGTTAGAATTTCGGTTCACTACGATGGAGAGTGGGTTAGTACTGCATACGTTGGTGGTAAGGCGAAAGAAATAGTGGTGTCAAAGGACATTACTTGTGAGGAGCTTCTACGTAGAGTGTATCGTCTCGTCAGGATAGATCAATGTGAGTATGAGATTATAATGAAGACTACAGATGAATCAAAATCGCCTGCTCAACCAGTACAGATAGTCGATGATGAGCACTTGGCATTTTTCATAGAACAGAGTCTAAGTTTGGATAAGAGCTTGAAGATGTATATTCCTAAACCGCGAATATCTTCTTCTAGAACTAGAAGGAAAATGAATGAACTTAGACTTTTGGTTAACTATGGTGGGAAGTGGAGCAATTCTATATACATCGGTGGTAAAACGAAAGGAATTACGGTTTCGGATAGTATTACATACAAGGAACTTGTGCAGAAACTGTATAATTCTCTTGAGGTAAATCCCAGTGAATATACGATCATAGTAACGACAGCATATGGATCAAAATCACCAACCCGACCGGTGGAGCTAATCGATGATGATGACCTGACATTTTTGATTGACGAGAGTCTGTCATTGGGCTCGGGCTCGAAAGTTCCTTTGTTCATCACACTCCAACGACGGCTATATCGTAGCAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

533

Amino Acids

60.47

Weight (kDa)

7.98

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 187 1.9e-44 TIR domain
TIR_2 PF13676 21 - 112 1.8e-13 TIR domain
TIR PF01582 198 - 286 3.8e-26 TIR domain
TIR_2 PF13676 201 - 282 1.6e-08 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1109
AccII CGCG 2 cut(s) 908, 1240
AciI CCGC 2 cut(s) 631, 1238
AclWI GGATC 2 cut(s) 326, 1462
AcsI RAATTY 6 cut(s) 25, 202, 235, 637, 677, 957
AcuI CTGAAG 1 cut(s) 390
AdeI CACNNNGTG 1 cut(s) 465
AfaI GTAC 4 cut(s) 345, 656, 991, 1152
AfeI AGCGCT 1 cut(s) 123
AfiI CCNNNNNNNGG 2 cut(s) 415, 1477
AgeI ACCGGT 2 cut(s) 911, 1474
AgsI TTSAA 8 cut(s) 25, 52, 474, 503, 637, 801, 836, 1219
AjnI CCWGG 1 cut(s) 730
AluBI AGCT 9 cut(s) 268, 434, 496, 529, 820, 1054, 1215, 1483, 1594
AluI AGCT 9 cut(s) 268, 434, 496, 529, 820, 1054, 1215, 1483, 1594
Alw21I GWGCWC 2 cut(s) 498, 1173
Alw26I GTCTC 2 cut(s) 872, 1079
AlwI GGATC 2 cut(s) 326, 1462
AlwNI CAGNNNCTG 1 cut(s) 1393
Ama87I CYCGRG 2 cut(s) 869, 1537
Aor51HI AGCGCT 1 cut(s) 123
AoxI GGCC 1 cut(s) 478
ApeKI GCWGC 2 cut(s) 191, 434
ApoI RAATTY 6 cut(s) 25, 202, 235, 637, 677, 957
AsiGI ACCGGT 2 cut(s) 911, 1474
Asp700I GAANNNNTTC 1 cut(s) 1246
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 1 cut(s) 478
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 774, 1455
AsuII TTCGAA 1 cut(s) 421
AvaI CYCGRG 2 cut(s) 869, 1537
BanII GRGCYC 3 cut(s) 498, 1538, 1544
BauI CACGAG 1 cut(s) 88
BbsI GAAGAC 2 cut(s) 90, 1121
Bbv12I GWGCWC 2 cut(s) 498, 1173
BbvCI CCTCAGC 1 cut(s) 553
BbvI GCAGC 2 cut(s) 178, 421
BccI CCATC 2 cut(s) 692, 968
BceAI ACGGC 1 cut(s) 1592
BciT130I CCWGG 1 cut(s) 732
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 2 cut(s) 872, 1079
BfaI CTAG 3 cut(s) 381, 1254, 1260
BfmI CTRYAG 1 cut(s) 1119
BfoI RGCGCY 1 cut(s) 125
BisI GCNGC 2 cut(s) 192, 435
BlsI GCNGC 2 cut(s) 193, 436
BmcAI AGTACT 1 cut(s) 991
Bme1390I CCNGG 2 cut(s) 482, 732
BmeT110I CYCGRG 2 cut(s) 869, 1537
BmgT120I GGNCC 1 cut(s) 478
BmiI GGNNCC 1 cut(s) 479
BmrFI CCNGG 2 cut(s) 482, 732
BmrI ACTGGG 2 cut(s) 471, 1413
BmsI GCATC 5 cut(s) 14, 148, 251, 883, 905
BmuI ACTGGG 2 cut(s) 471, 1413
BoxI GACNNNNGTC 1 cut(s) 1521
BpiI GAAGAC 2 cut(s) 90, 1121
BpmI CTGGAG 1 cut(s) 21
Bpu10I CCTNAGC 1 cut(s) 553
Bpu14I TTCGAA 1 cut(s) 421
BpuEI CTTGAG 4 cut(s) 146, 170, 728, 1427
BpuMI CCSGG 1 cut(s) 482
Bsa29I ATCGAT 1 cut(s) 1488
BsaAI YACGTR 2 cut(s) 654, 1061
BsaBI GATNNNNATC 1 cut(s) 1459
BsaJI CCNNGG 3 cut(s) 251, 289, 730
BsaWI WCCGGW 2 cut(s) 911, 1474
BsaXI ACNNNNNCTCC 2 cut(s) 969, 999
Bsc4I CCNNNNNNNGG 2 cut(s) 415, 1477
Bse118I RCCGGY 3 cut(s) 134, 911, 1474
Bse1I ACTGG 3 cut(s) 466, 1148, 1419
Bse8I GATNNNNATC 1 cut(s) 1459
BseBI CCWGG 1 cut(s) 732
BseCI ATCGAT 1 cut(s) 1488
BseDI CCNNGG 3 cut(s) 251, 289, 730
BseGI GGATG 4 cut(s) 409, 820, 848, 898
BseJI GATNNNNATC 1 cut(s) 1459
BseLI CCNNNNNNNGG 2 cut(s) 415, 1477
BseMII CTCAG 1 cut(s) 567
BseNI ACTGG 3 cut(s) 466, 1148, 1419
BseRI GAGGAG 5 cut(s) 24, 93, 278, 877, 1064
BseXI GCAGC 2 cut(s) 178, 421
BsgI GTGCAG 1 cut(s) 1406
Bsh1236I CGCG 2 cut(s) 908, 1240
Bsh1285I CGRYCG 2 cut(s) 912, 1475
BshFI GGCC 1 cut(s) 480
BshTI ACCGGT 2 cut(s) 911, 1474
BshVI ATCGAT 1 cut(s) 1488
BsiEI CGRYCG 2 cut(s) 912, 1475
BsiHKAI GWGCWC 2 cut(s) 498, 1173
BsiHKCI CYCGRG 2 cut(s) 869, 1537
BsiSI CCGG 4 cut(s) 135, 481, 912, 1475
BsiWI CGTACG 2 cut(s) 343, 654
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 2 cut(s) 415, 1477
BsmAI GTCTC 2 cut(s) 872, 1079
BsmBI CGTCTC 1 cut(s) 1079
BsmFI GGGAC 1 cut(s) 497
BsmI GAATGC 1 cut(s) 842
BsnI GGCC 1 cut(s) 480
BsoBI CYCGRG 2 cut(s) 869, 1537
Bsp119I TTCGAA 1 cut(s) 421
Bsp1286I GDGCHC 4 cut(s) 498, 1173, 1538, 1544
Bsp143I GATC 4 cut(s) 331, 1087, 1431, 1454
Bsp19I CCATGG 2 cut(s) 251, 289
Bsp68I TCGCGA 1 cut(s) 908
BspACI CCGC 2 cut(s) 631, 1238
BspANI GGCC 1 cut(s) 480
BspCNI CTCAG 1 cut(s) 566
BspDI ATCGAT 1 cut(s) 1488
BspFNI CGCG 2 cut(s) 908, 1240
BspLI GGNNCC 1 cut(s) 479
BspPI GGATC 2 cut(s) 326, 1462
BspT104I TTCGAA 1 cut(s) 421
BsrFI RCCGGY 3 cut(s) 134, 911, 1474
BsrI ACTGG 3 cut(s) 466, 1148, 1419
BssAI RCCGGY 3 cut(s) 134, 911, 1474
BssECI CCNNGG 3 cut(s) 251, 289, 730
BssMI GATC 4 cut(s) 331, 1087, 1431, 1454
BssSI CACGAG 1 cut(s) 88
BssT1I CCWWGG 2 cut(s) 251, 289
Bst2BI CACGAG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 732
Bst4CI ACNGT 4 cut(s) 307, 904, 1354, 1395
BstBAI YACGTR 2 cut(s) 654, 1061
BstBI TTCGAA 1 cut(s) 421
BstC8I GCNNGC 3 cut(s) 136, 1140, 1592
BstDEI CTNAG 3 cut(s) 553, 1199, 1280
BstDSI CCRYGG 2 cut(s) 251, 289
BstF5I GGATG 4 cut(s) 409, 820, 848, 898
BstFNI CGCG 2 cut(s) 908, 1240
BstH2I RGCGCY 1 cut(s) 125
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 4 cut(s) 334, 1090, 1434, 1457
BstMAI GTCTC 2 cut(s) 872, 1079
BstMBI GATC 4 cut(s) 331, 1087, 1431, 1454
BstMCI CGRYCG 2 cut(s) 912, 1475
BstNI CCWGG 1 cut(s) 732
BstPAI GACNNNNGTC 1 cut(s) 1521
BstSCI CCNGG 2 cut(s) 480, 730
BstSFI CTRYAG 1 cut(s) 1119
BstSNI TACGTA 2 cut(s) 654, 1061
BstUI CGCG 2 cut(s) 908, 1240
BstV1I GCAGC 2 cut(s) 178, 421
BstV2I GAAGAC 2 cut(s) 90, 1121
Bsu15I ATCGAT 1 cut(s) 1488
BsuRI GGCC 1 cut(s) 480
BsuTUI ATCGAT 1 cut(s) 1488
BtgI CCRYGG 2 cut(s) 251, 289
BtsCI GGATG 4 cut(s) 409, 820, 848, 898
BtsIMutI CAGTG 3 cut(s) 459, 662, 1426
BtuMI TCGCGA 1 cut(s) 908
Cac8I GCNNGC 3 cut(s) 136, 1140, 1592
CaiI CAGNNNCTG 1 cut(s) 1393
CfoI GCGC 1 cut(s) 124
Cfr10I RCCGGY 3 cut(s) 134, 911, 1474
Cfr13I GGNCC 1 cut(s) 478
ClaI ATCGAT 1 cut(s) 1488
Csp6I GTAC 4 cut(s) 344, 655, 990, 1151
CspAI ACCGGT 2 cut(s) 911, 1474
CviAII CATG 3 cut(s) 252, 290, 841
CviQI GTAC 4 cut(s) 344, 655, 990, 1151
DdeI CTNAG 3 cut(s) 553, 1199, 1280
DpnI GATC 4 cut(s) 333, 1089, 1433, 1456
DpnII GATC 4 cut(s) 331, 1087, 1431, 1454
DraI TTTAAA 1 cut(s) 691
DraIII CACNNNGTG 1 cut(s) 465
Ecl136II GAGCTC 1 cut(s) 496
Eco105I TACGTA 2 cut(s) 654, 1061
Eco130I CCWWGG 2 cut(s) 251, 289
Eco24I GRGCYC 3 cut(s) 498, 1538, 1544
Eco32I GATATC 1 cut(s) 350
Eco47III AGCGCT 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 496
Eco57I CTGAAG 1 cut(s) 390
Eco88I CYCGRG 2 cut(s) 869, 1537
EcoICRI GAGCTC 1 cut(s) 496
EcoRI GAATTC 2 cut(s) 25, 202
EcoRII CCWGG 1 cut(s) 730
EcoRV GATATC 1 cut(s) 350
EcoT14I CCWWGG 2 cut(s) 251, 289
EcoT22I ATGCAT 2 cut(s) 842, 898
EcoT38I GRGCYC 3 cut(s) 498, 1538, 1544
ErhI CCWWGG 2 cut(s) 251, 289
Esp3I CGTCTC 1 cut(s) 1079
FaeI CATG 3 cut(s) 255, 293, 844
FaqI GGGAC 1 cut(s) 497
FatI CATG 3 cut(s) 251, 289, 840
FauI CCCGC 1 cut(s) 638
FauNDI CATATG 1 cut(s) 1450
Fnu4HI GCNGC 2 cut(s) 192, 435
FokI GGATG 4 cut(s) 416, 827, 835, 905
FriOI GRGCYC 3 cut(s) 498, 1538, 1544
Fsp4HI GCNGC 2 cut(s) 192, 435
FspBI CTAG 3 cut(s) 381, 1254, 1260
GlaI GCGC 1 cut(s) 123
GluI GCNGC 2 cut(s) 192, 435
GsuI CTGGAG 1 cut(s) 21
HaeII RGCGCY 1 cut(s) 125
HaeIII GGCC 1 cut(s) 480
HapII CCGG 4 cut(s) 135, 481, 912, 1475
HhaI GCGC 1 cut(s) 124
Hin1II CATG 3 cut(s) 255, 293, 844
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HincII GTYRAC 1 cut(s) 1294
HindII GTYRAC 1 cut(s) 1294
HindIII AAGCTT 2 cut(s) 527, 1592
HinfI GANTC 8 cut(s) 277, 488, 667, 755, 865, 1127, 1195, 1522
HpaI GTTAAC 1 cut(s) 1294
HpaII CCGG 4 cut(s) 135, 481, 912, 1475
HphI GGTGA 2 cut(s) 774, 1455
Hpy166II GTNNAC 2 cut(s) 967, 1294
Hpy188I TCNGA 4 cut(s) 201, 340, 493, 1360
Hpy188III TCNNGA 7 cut(s) 38, 671, 869, 907, 1081, 1254, 1406
Hpy8I GTNNAC 2 cut(s) 967, 1294
Hpy99I CGWCG 2 cut(s) 250, 1578
HpyAV CCTTC 2 cut(s) 417, 1257
HpyCH4III ACNGT 4 cut(s) 307, 904, 1354, 1395
HpyCH4IV ACGT 4 cut(s) 342, 653, 999, 1060
HpyCH4V TGCA 8 cut(s) 194, 215, 242, 535, 840, 896, 995, 1387
HpyF3I CTNAG 3 cut(s) 553, 1199, 1280
HpySE526I ACGT 4 cut(s) 342, 653, 999, 1060
Hsp92II CATG 3 cut(s) 255, 293, 844
HspAI GCGC 1 cut(s) 122
KroI GCCGGC 1 cut(s) 134
KroNI GCCGGC 1 cut(s) 136
KspAI GTTAAC 1 cut(s) 1294
Kzo9I GATC 4 cut(s) 331, 1087, 1431, 1454
LmnI GCTCC 5 cut(s) 166, 265, 1051, 1311, 1480
Lsp1109I GCAGC 2 cut(s) 178, 421
LweI GCATC 5 cut(s) 14, 148, 251, 883, 905
MaeI CTAG 3 cut(s) 381, 1254, 1260
MaeII ACGT 4 cut(s) 342, 653, 999, 1060
MaeIII GTNAC 3 cut(s) 772, 923, 1438
MalI GATC 4 cut(s) 333, 1089, 1433, 1456
MboI GATC 4 cut(s) 331, 1087, 1431, 1454
MfeI CAATTG 1 cut(s) 747
MhlI GDGCHC 4 cut(s) 498, 1173, 1538, 1544
MlyI GAGTC 3 cut(s) 874, 1204, 1531
MmeI TCCRAC 2 cut(s) 876, 1594
Mph1103I ATGCAT 2 cut(s) 842, 898
MreI CGCCGGCG 1 cut(s) 134
MroNI GCCGGC 1 cut(s) 134
MroXI GAANNNNTTC 1 cut(s) 1246
MseI TTAA 4 cut(s) 282, 440, 690, 1293
MslI CAYNNNNRTG 3 cut(s) 114, 156, 972
MspI CCGG 4 cut(s) 135, 481, 912, 1475
MspR9I CCNGG 2 cut(s) 482, 732
MunI CAATTG 1 cut(s) 747
Mva1269I GAATGC 1 cut(s) 842
MvaI CCWGG 1 cut(s) 732
MvnI CGCG 2 cut(s) 908, 1240
NaeI GCCGGC 1 cut(s) 136
NciI CCSGG 1 cut(s) 482
NcoI CCATGG 2 cut(s) 251, 289
NdeI CATATG 1 cut(s) 1450
NdeII GATC 4 cut(s) 331, 1087, 1431, 1454
NgoMIV GCCGGC 1 cut(s) 134
NlaIII CATG 3 cut(s) 255, 293, 844
NlaIV GGNNCC 1 cut(s) 479
NmuCI GTSAC 1 cut(s) 772
NruI TCGCGA 1 cut(s) 908
NsiI ATGCAT 2 cut(s) 842, 898
NspV TTCGAA 1 cut(s) 421
PaeR7I CTCGAG 1 cut(s) 869
PcsI WCGNNNNNNNCGW 2 cut(s) 905, 1582
PctI GAATGC 1 cut(s) 842
PdiI GCCGGC 1 cut(s) 136
PdmI GAANNNNTTC 1 cut(s) 1246
PfeI GAWTC 5 cut(s) 277, 488, 667, 755, 1127
Pfl23II CGTACG 2 cut(s) 343, 654
PinAI ACCGGT 2 cut(s) 911, 1474
PkrI GCNGC 2 cut(s) 193, 436
PleI GAGTC 3 cut(s) 873, 1203, 1530
PpsI GAGTC 3 cut(s) 873, 1203, 1530
Ppu21I YACGTR 2 cut(s) 654, 1061
PshAI GACNNNNGTC 1 cut(s) 1521
PsiI TTATAA 1 cut(s) 1109
Psp124BI GAGCTC 1 cut(s) 498
Psp6I CCWGG 1 cut(s) 730
PspGI CCWGG 1 cut(s) 730
PspLI CGTACG 2 cut(s) 343, 654
PspN4I GGNNCC 1 cut(s) 479
PspPI GGNCC 1 cut(s) 478
PstNI CAGNNNCTG 1 cut(s) 1393
RruI TCGCGA 1 cut(s) 908
RsaI GTAC 4 cut(s) 345, 656, 991, 1152
RsaNI GTAC 4 cut(s) 344, 655, 990, 1151
RseI CAYNNNNRTG 3 cut(s) 114, 156, 972
SacI GAGCTC 1 cut(s) 498
SaqAI TTAA 4 cut(s) 282, 440, 690, 1293
SatI GCNGC 2 cut(s) 192, 435
Sau3AI GATC 4 cut(s) 331, 1087, 1431, 1454
Sau96I GGNCC 1 cut(s) 478
ScaI AGTACT 1 cut(s) 991
SchI GAGTC 3 cut(s) 874, 1204, 1531
ScrFI CCNGG 2 cut(s) 482, 732
SduI GDGCHC 4 cut(s) 498, 1173, 1538, 1544
SfaNI GCATC 5 cut(s) 14, 148, 251, 883, 905
SfcI CTRYAG 1 cut(s) 1119
Sfr274I CTCGAG 1 cut(s) 869
SfuI TTCGAA 1 cut(s) 421
SgrAI CRCCGGYG 1 cut(s) 134
SlaI CTCGAG 1 cut(s) 869
SmiMI CAYNNNNRTG 3 cut(s) 114, 156, 972
SmlI CTYRAG 5 cut(s) 125, 185, 707, 869, 1406
SmoI CTYRAG 5 cut(s) 125, 185, 707, 869, 1406
SnaBI TACGTA 2 cut(s) 654, 1061
SsiI CCGC 2 cut(s) 631, 1238
SspMI CTAG 3 cut(s) 381, 1254, 1260
SstI GAGCTC 1 cut(s) 498
StyD4I CCNGG 2 cut(s) 480, 730
StyI CCWWGG 2 cut(s) 251, 289
TaaI ACNGT 4 cut(s) 307, 904, 1354, 1395
TaiI ACGT 4 cut(s) 345, 656, 1002, 1063
TaqI TCGA 9 cut(s) 76, 206, 330, 421, 870, 899, 1161, 1488, 1544
TatI WGTACW 2 cut(s) 989, 1150
TfiI GAWTC 5 cut(s) 277, 488, 667, 755, 1127
Tru1I TTAA 4 cut(s) 282, 440, 690, 1293
Tru9I TTAA 4 cut(s) 282, 440, 690, 1293
TscAI CASTG 3 cut(s) 466, 669, 1426
TseFI GTSAC 1 cut(s) 772
TseI GCWGC 2 cut(s) 191, 434
Tsp45I GTSAC 1 cut(s) 772
TspDTI ATGAA 8 cut(s) 18, 132, 1127, 1140, 1174, 1286, 1290, 1549
TspGWI ACGGA 2 cut(s) 538, 604
TspRI CASTG 3 cut(s) 466, 669, 1426
XapI RAATTY 6 cut(s) 25, 202, 235, 637, 677, 957
XbaI TCTAGA 1 cut(s) 1253
XhoI CTCGAG 1 cut(s) 869
XmnI GAANNNNTTC 1 cut(s) 1246
XspI CTAG 3 cut(s) 381, 1254, 1260
ZrmI AGTACT 1 cut(s) 991
Zsp2I ATGCAT 2 cut(s) 842, 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.