Rw5G023950

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000885
Physical Location & Seq
Forward (+)
33956554 .. 33964232
7679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023950.1

Sequence Viewer

Length: 1107 bp
ATGACGTCCGATCATCTATGGACCGAACTCCGTGCCATCCATGACGAATTGAAGGAATTGCGGTTTCTGCGACTCCGGCGGCCTGCACTTTCAGTGACGTCTGATGATCTATCCACAAAACTCCGTGCTGTAGGGGACAGATGGAATAAATTCCGGAAATCCTACCACAATGAAATAGGGACCATCCTCATCCTGGACTGGAAATACGTGGTTAGGGTAGAACTTCTATATGGACTCGTTCTGGCTCTTGACTTAGCTGAGAATATTCATTGCGTTTCCGAAAGACAACGTCCACAGATCGAGTTAGACGTGACTGAGATACTTGGAGTCTACTCCAGAGCTCTAGAATATCTTCACACTGGAAGTTTGGCACGGTCGGATAATAATGTAGTAGAAAAAACAGTTCTGCCAATATTCTACGGTGTTGATCCCTCCAACGTACGACATCAAACAGGAAGTTTTGCAGAAGCCTTTGCAGACCATGATAAAAAGTACAAGGAAGACCCAGAAAAAGTAAAAAGGTGGAGGGATGCTTTAACGAAACTGGCAAATCTCGCTGGCTGGGATTCAAAGCACTGGCCCGAAGCAAAACTCATTCGAGAAGTTGTTGATGAGATATGGAGGAAATTGTATTCTTCTTTTTCAAAACCTGCCATTACAAATGTGCCATCAACACCTTCTTGGTCCTCTTCTTGTCGACAGAAGCATGATGTTTTTCTAAGCTTTAGAGGGAAGGATACCCGCTTGAATTTTTTGGACCATCTATATGATGCATTAATTCAGCAAAAAATTTCCACCTTTAGGGATGACAAAGATCTTCAGAAAGGACTGTCCATTCGGAAACAACTCGACAATGCAATTGAGGGATCAAAGGTTTGGGTTGTCATTGTTTCACCAGAGTATGCTTCTTCAAAATGGTGCTTGGATGAGCTTTCCAAGATTCTTGAATGTAGAAAAGCATCTAAAGTCGAAAATAAGAATCAAACTTTGATGAAGGCAGTTGCAGAACATGAAGAACAAGCTCATACTTTGGTGAATGGAGACAAAACACACAAGGGGAAGGCTAGTTTGCGTAAGGAAACCATCAGTAATCATAATAAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

368

Amino Acids

42.57

Weight (kDa)

9.09

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 132 - 216 9.2e-19 TIR domain
TIR PF01582 235 - 322 1.2e-29 TIR domain
TIR_2 PF13676 238 - 319 4e-15 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 8, 101
Acc36I ACCTGC 1 cut(s) 658
AccI GTMKAC 2 cut(s) 330, 697
AccIII TCCGGA 1 cut(s) 153
AciI CCGC 3 cut(s) 61, 79, 742
AclWI GGATC 2 cut(s) 422, 874
AcsI RAATTY 3 cut(s) 149, 748, 789
AcuI CTGAAG 1 cut(s) 803
AcyI GRCGYC 2 cut(s) 5, 98
AfaI GTAC 2 cut(s) 441, 494
AfiI CCNNNNNNNGG 2 cut(s) 193, 801
AgsI TTSAA 6 cut(s) 52, 570, 645, 748, 912, 947
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 5 cut(s) 257, 341, 723, 931, 1022
AluI AGCT 5 cut(s) 257, 341, 723, 931, 1022
Alw21I GWGCWC 1 cut(s) 343
Alw26I GTCTC 1 cut(s) 1035
AlwI GGATC 2 cut(s) 422, 874
Aor13HI TCCGGA 1 cut(s) 153
AoxI GGCC 2 cut(s) 80, 578
ApoI RAATTY 3 cut(s) 149, 748, 789
ArsI GACNNNNNNTTYG 2 cut(s) 819, 851
AseI ATTAAT 1 cut(s) 776
Asp700I GAANNNNTTC 2 cut(s) 149, 351
AspS9I GGNCC 5 cut(s) 21, 180, 579, 684, 757
AsuHPI GGTGA 2 cut(s) 885, 1045
AvaII GGWCC 4 cut(s) 21, 180, 684, 757
BanII GRGCYC 1 cut(s) 343
BbsI GAAGAC 1 cut(s) 507
Bbv12I GWGCWC 1 cut(s) 343
BccI CCATC 6 cut(s) 44, 135, 191, 676, 768, 1091
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BciT130I CCWGG 1 cut(s) 194
BciVI GTATCC 1 cut(s) 730
BcoDI GTCTC 1 cut(s) 1035
BfaI CTAG 2 cut(s) 344, 1065
BfmI CTRYAG 1 cut(s) 129
BfuAI ACCTGC 1 cut(s) 658
BfuI GTATCC 1 cut(s) 730
BglII AGATCT 1 cut(s) 814
BisI GCNGC 1 cut(s) 80
BlsI GCNGC 1 cut(s) 81
Bme1390I CCNGG 1 cut(s) 194
Bme18I GGWCC 4 cut(s) 21, 180, 684, 757
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 5 cut(s) 21, 180, 579, 684, 757
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 3 cut(s) 520, 760, 968
BpiI GAAGAC 1 cut(s) 507
BpmI CTGGAG 1 cut(s) 319
BsaAI YACGTR 1 cut(s) 208
BsaHI GRCGYC 2 cut(s) 5, 98
BsaWI WCCGGW 1 cut(s) 153
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 801
Bse1I ACTGG 4 cut(s) 203, 364, 549, 581
Bse3DI GCAATG 1 cut(s) 268
BseAI TCCGGA 1 cut(s) 153
BseBI CCWGG 1 cut(s) 194
BseGI GGATG 6 cut(s) 36, 183, 189, 535, 811, 931
BseLI CCNNNNNNNGG 2 cut(s) 193, 801
BseMI GCAATG 1 cut(s) 268
BseMII CTCAG 2 cut(s) 249, 306
BseNI ACTGG 4 cut(s) 203, 364, 549, 581
BseYI CCCAGC 1 cut(s) 561
BsgI GTGCAG 1 cut(s) 69
Bsh1285I CGRYCG 1 cut(s) 377
BshFI GGCC 2 cut(s) 82, 580
BsiEI CGRYCG 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 343
BsiSI CCGG 2 cut(s) 76, 154
BsiWI CGTACG 1 cut(s) 439
BslFI GGGAC 2 cut(s) 149, 193
BslI CCNNNNNNNGG 2 cut(s) 193, 801
BsmAI GTCTC 1 cut(s) 1035
BsmFI GGGAC 2 cut(s) 149, 193
BsnI GGCC 2 cut(s) 82, 580
Bsp1286I GDGCHC 1 cut(s) 343
Bsp13I TCCGGA 1 cut(s) 153
Bsp143I GATC 6 cut(s) 10, 106, 297, 427, 814, 866
BspACI CCGC 3 cut(s) 61, 79, 742
BspANI GGCC 2 cut(s) 82, 580
BspCNI CTCAG 2 cut(s) 250, 307
BspEI TCCGGA 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 181
BspMI ACCTGC 1 cut(s) 658
BspPI GGATC 2 cut(s) 422, 874
BsrDI GCAATG 1 cut(s) 268
BsrI ACTGG 4 cut(s) 203, 364, 549, 581
BssMI GATC 6 cut(s) 10, 106, 297, 427, 814, 866
BssNI GRCGYC 2 cut(s) 5, 98
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 4 cut(s) 375, 403, 422, 831
Bst6I CTCTTC 1 cut(s) 694
BstACI GRCGYC 2 cut(s) 5, 98
BstBAI YACGTR 1 cut(s) 208
BstC8I GCNNGC 2 cut(s) 84, 559
BstDEI CTNAG 4 cut(s) 253, 258, 315, 719
BstF5I GGATG 6 cut(s) 36, 183, 189, 535, 811, 931
BstKTI GATC 6 cut(s) 13, 109, 300, 430, 817, 869
BstMAI GTCTC 1 cut(s) 1035
BstMBI GATC 6 cut(s) 10, 106, 297, 427, 814, 866
BstMCI CGRYCG 1 cut(s) 377
BstMWI GCNNNNNNNGC 3 cut(s) 67, 76, 554
BstNI CCWGG 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 192
BstSFI CTRYAG 1 cut(s) 129
BstV2I GAAGAC 1 cut(s) 507
BstX2I RGATCY 1 cut(s) 814
BstYI RGATCY 1 cut(s) 814
BsuI GTATCC 1 cut(s) 730
BsuRI GGCC 2 cut(s) 82, 580
BtrI CACGTC 1 cut(s) 310
BtsCI GGATG 6 cut(s) 36, 183, 189, 535, 811, 931
BtsIMutI CAGTG 3 cut(s) 99, 357, 574
BveI ACCTGC 1 cut(s) 658
Cac8I GCNNGC 2 cut(s) 84, 559
Cfr13I GGNCC 5 cut(s) 21, 180, 579, 684, 757
Csp6I GTAC 2 cut(s) 440, 493
CviAII CATG 4 cut(s) 41, 482, 707, 1010
CviQI GTAC 2 cut(s) 440, 493
DdeI CTNAG 4 cut(s) 253, 258, 315, 719
DpnI GATC 6 cut(s) 12, 108, 299, 429, 816, 868
DpnII GATC 6 cut(s) 10, 106, 297, 427, 814, 866
Eam1104I CTCTTC 1 cut(s) 694
EarI CTCTTC 1 cut(s) 694
Ecl136II GAGCTC 1 cut(s) 341
Eco24I GRGCYC 1 cut(s) 343
Eco47I GGWCC 4 cut(s) 21, 180, 684, 757
Eco53kI GAGCTC 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 803
EcoICRI GAGCTC 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 192
EcoT22I ATGCAT 1 cut(s) 775
EcoT38I GRGCYC 1 cut(s) 343
FaeI CATG 4 cut(s) 44, 485, 710, 1013
FaqI GGGAC 2 cut(s) 149, 193
FatI CATG 4 cut(s) 40, 481, 706, 1009
FauI CCCGC 1 cut(s) 749
FblI GTMKAC 2 cut(s) 330, 697
Fnu4HI GCNGC 1 cut(s) 80
FokI GGATG 6 cut(s) 23, 170, 176, 542, 818, 938
FriOI GRGCYC 1 cut(s) 343
Fsp4HI GCNGC 1 cut(s) 80
FspBI CTAG 2 cut(s) 344, 1065
GluI GCNGC 1 cut(s) 80
GsaI CCCAGC 1 cut(s) 565
GsuI CTGGAG 1 cut(s) 319
HaeIII GGCC 2 cut(s) 82, 580
HapII CCGG 2 cut(s) 76, 154
Hin1I GRCGYC 2 cut(s) 5, 98
Hin1II CATG 4 cut(s) 44, 485, 710, 1013
HincII GTYRAC 1 cut(s) 698
HindII GTYRAC 1 cut(s) 698
HindIII AAGCTT 1 cut(s) 721
HinfI GANTC 6 cut(s) 72, 234, 327, 566, 940, 979
HpaII CCGG 2 cut(s) 76, 154
HphI GGTGA 2 cut(s) 885, 1045
Hpy166II GTNNAC 3 cut(s) 293, 331, 698
Hpy188I TCNGA 6 cut(s) 10, 103, 280, 379, 822, 840
Hpy188III TCNNGA 6 cut(s) 154, 248, 336, 344, 599, 944
Hpy8I GTNNAC 3 cut(s) 293, 331, 698
HpyAV CCTTC 5 cut(s) 46, 687, 727, 988, 1054
HpyCH4III ACNGT 4 cut(s) 375, 403, 422, 831
HpyCH4IV ACGT 6 cut(s) 5, 98, 207, 289, 309, 438
HpyCH4V TGCA 6 cut(s) 86, 464, 476, 773, 857, 1004
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 76, 554
HpyF3I CTNAG 4 cut(s) 253, 258, 315, 719
HpySE526I ACGT 6 cut(s) 5, 98, 207, 289, 309, 438
Hsp92I GRCGYC 2 cut(s) 5, 98
Hsp92II CATG 4 cut(s) 44, 485, 710, 1013
Kpn2I TCCGGA 1 cut(s) 153
Kzo9I GATC 6 cut(s) 10, 106, 297, 427, 814, 866
LweI GCATC 3 cut(s) 520, 760, 968
MaeI CTAG 2 cut(s) 344, 1065
MaeII ACGT 6 cut(s) 5, 98, 207, 289, 309, 438
MaeIII GTNAC 2 cut(s) 94, 310
MalI GATC 6 cut(s) 12, 108, 299, 429, 816, 868
MboI GATC 6 cut(s) 10, 106, 297, 427, 814, 866
MboII GAAGA 7 cut(s) 344, 512, 627, 681, 809, 900, 1025
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 1 cut(s) 814
MhlI GDGCHC 1 cut(s) 343
MluCI AATT 8 cut(s) 47, 56, 149, 626, 748, 777, 789, 858
MlyI GAGTC 3 cut(s) 66, 228, 336
MmeI TCCRAC 2 cut(s) 357, 459
MnlI CCTC 7 cut(s) 197, 442, 519, 615, 697, 722, 856
Mph1103I ATGCAT 1 cut(s) 775
MroI TCCGGA 1 cut(s) 153
MroXI GAANNNNTTC 2 cut(s) 149, 351
MseI TTAA 2 cut(s) 536, 776
MslI CAYNNNNRTG 1 cut(s) 765
MspI CCGG 2 cut(s) 76, 154
MspR9I CCNGG 1 cut(s) 194
MunI CAATTG 1 cut(s) 858
MvaI CCWGG 1 cut(s) 194
MwoI GCNNNNNNNGC 3 cut(s) 67, 76, 554
NdeII GATC 6 cut(s) 10, 106, 297, 427, 814, 866
NlaIII CATG 4 cut(s) 44, 485, 710, 1013
NlaIV GGNNCC 1 cut(s) 181
NmuCI GTSAC 2 cut(s) 94, 310
NsiI ATGCAT 1 cut(s) 775
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PdmI GAANNNNTTC 2 cut(s) 149, 351
PfeI GAWTC 3 cut(s) 566, 940, 979
Pfl23II CGTACG 1 cut(s) 439
PflFI GACNNNGTC 1 cut(s) 288
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 1 cut(s) 81
PleI GAGTC 3 cut(s) 66, 228, 335
PpsI GAGTC 3 cut(s) 66, 228, 335
Ppu21I YACGTR 1 cut(s) 208
PshBI ATTAAT 1 cut(s) 776
Psp124BI GAGCTC 1 cut(s) 343
Psp6I CCWGG 1 cut(s) 192
PspFI CCCAGC 1 cut(s) 561
PspGI CCWGG 1 cut(s) 192
PspLI CGTACG 1 cut(s) 439
PspN4I GGNNCC 1 cut(s) 181
PspPI GGNCC 5 cut(s) 21, 180, 579, 684, 757
PsuI RGATCY 1 cut(s) 814
PsyI GACNNNGTC 1 cut(s) 288
RsaI GTAC 2 cut(s) 441, 494
RsaNI GTAC 2 cut(s) 440, 493
RseI CAYNNNNRTG 1 cut(s) 765
SacI GAGCTC 1 cut(s) 343
SalI GTCGAC 1 cut(s) 696
SaqAI TTAA 2 cut(s) 536, 776
SatI GCNGC 1 cut(s) 80
Sau3AI GATC 6 cut(s) 10, 106, 297, 427, 814, 866
Sau96I GGNCC 5 cut(s) 21, 180, 579, 684, 757
SchI GAGTC 3 cut(s) 66, 228, 336
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 1 cut(s) 343
SfaNI GCATC 3 cut(s) 520, 760, 968
SfcI CTRYAG 1 cut(s) 129
SinI GGWCC 4 cut(s) 21, 180, 684, 757
SmiMI CAYNNNNRTG 1 cut(s) 765
Sse9I AATT 8 cut(s) 47, 56, 149, 626, 748, 777, 789, 858
SsiI CCGC 3 cut(s) 61, 79, 742
SspI AATATT 2 cut(s) 265, 414
SspMI CTAG 2 cut(s) 344, 1065
SstI GAGCTC 1 cut(s) 343
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 4 cut(s) 375, 403, 422, 831
TaiI ACGT 6 cut(s) 8, 101, 210, 292, 312, 441
TaqI TCGA 5 cut(s) 300, 598, 697, 849, 969
TaqII GACCGA 1 cut(s) 38
TasI AATT 8 cut(s) 47, 56, 149, 626, 748, 777, 789, 858
TatI WGTACW 1 cut(s) 492
TauI GCSGC 1 cut(s) 82
TfiI GAWTC 3 cut(s) 566, 940, 979
Tru1I TTAA 2 cut(s) 536, 776
Tru9I TTAA 2 cut(s) 536, 776
TscAI CASTG 3 cut(s) 99, 364, 581
TseFI GTSAC 2 cut(s) 94, 310
Tsp45I GTSAC 2 cut(s) 94, 310
TspDTI ATGAA 4 cut(s) 186, 257, 1007, 1026
TspGWI ACGGA 2 cut(s) 20, 113
TspRI CASTG 3 cut(s) 99, 364, 581
Tth111I GACNNNGTC 1 cut(s) 288
VpaK11BI GGWCC 4 cut(s) 21, 180, 684, 757
VspI ATTAAT 1 cut(s) 776
XapI RAATTY 3 cut(s) 149, 748, 789
XbaI TCTAGA 1 cut(s) 343
XcmI CCANNNNNNNNNTGG 1 cut(s) 190
XmiI GTMKAC 2 cut(s) 330, 697
XmnI GAANNNNTTC 2 cut(s) 149, 351
XspI CTAG 2 cut(s) 344, 1065
ZraI GACGTC 2 cut(s) 6, 99
Zsp2I ATGCAT 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.