RchiOBHm_Chr5g0035701

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000885
Physical Location & Seq
Reverse (-)
29688731 .. 29693276
4546 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31454

Sequence Viewer

Length: 828 bp
ATGCACTTGAAGCATCCATTGCCGAATTCCTCTGGAAGCAGGAGTATTGAAGTTAGAGTTTTGGTTAACTATGATGGGGAGTGGGTGAATTCTACATATGTCGGTGGCAAAACTAAAGGAATAATAATCTCAGAGGACATTACGTATCAAGAACTTGTTGATAGAGTGAGTGGTGTTGTGGAAGTTGATTCAAATGAATATAAGTTGATTCTGAAGACTGCATATAAATCAAATCTACCTACTCAACCTGTGGAGATAATCGATGATGAGGGCCTTGCATTTTTTATCCACGATGAGAATCTTTCACTGGGTATGAGCTCCATATTTACTTTGTGCACTACTCTTGAAAGACAGGCATTTCCTAATGGAAGGATGGAGTCATCACAAAGTGGTACAACACATCTTAGAAGATTTGGTACCTATCCGAGTTGCGTTCATTGTGTGACACCTGATCAGGTGCCTCATCTTCATACTCAAGTTGGACCATCGTCACCAAATGTGCCTTGTATGCAGCAGCAGCAACAACATTCTTACCAACAGACGACGTTGCAGCCTTGCTACGAGCACTATCAACCATCCTTTCAGCAGCCACAGCCTCTGTACCAACAGCTTCATCCTTTCTACGTGCAGCAGCAGCCTCAGTACCAACAGCCGAATCCTTCCTACATGCAGCAGCCGCCGCCAATCCATCAAGTGCCTTACTCGCTCAAGATGTTCTGGAATGATGCAACCCAAATGTTTCAAGATTTCAACTTTTGGCAATTTAGTTTCGGCATCATTTTTTTAGGAAATATTTTCTATTTCTTAAGGCTTGGTAGAAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

275

Amino Acids

31.76

Weight (kDa)

5.96

Isoelectric Point (pI)

61.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 416
AccB1I GGYRCC 2 cut(s) 416, 457
AciI CCGC 2 cut(s) 677, 680
AcsI RAATTY 2 cut(s) 25, 88
AcuI CTGAAG 1 cut(s) 233
AdeI CACNNNGTG 1 cut(s) 389
AfaI GTAC 4 cut(s) 394, 418, 602, 644
AflII CTTAAG 1 cut(s) 805
AgsI TTSAA 6 cut(s) 10, 50, 192, 347, 743, 751
AluBI AGCT 2 cut(s) 318, 610
AluI AGCT 2 cut(s) 318, 610
Alw21I GWGCWC 3 cut(s) 320, 338, 567
Alw44I GTGCAC 1 cut(s) 334
AlwNI CAGNNNCTG 1 cut(s) 598
AoxI GGCC 1 cut(s) 271
ApaLI GTGCAC 1 cut(s) 334
ApoI RAATTY 2 cut(s) 25, 88
Asp718I GGTACC 1 cut(s) 416
AspS9I GGNCC 2 cut(s) 271, 482
AsuHPI GGTGA 2 cut(s) 97, 483
AvaII GGWCC 1 cut(s) 482
BaeGI GKGCMC 1 cut(s) 338
BanI GGYRCC 2 cut(s) 416, 457
BanII GRGCYC 1 cut(s) 320
BarI GAAGNNNNNNTAC 2 cut(s) 400, 432
BbsI GAAGAC 1 cut(s) 221
Bbv12I GWGCWC 3 cut(s) 320, 338, 567
BccI CCATC 5 cut(s) 68, 367, 493, 583, 696
BclI TGATCA 1 cut(s) 451
BfrI CTTAAG 1 cut(s) 805
Bme18I GGWCC 1 cut(s) 482
BmgT120I GGNCC 2 cut(s) 271, 482
BmiI GGNNCC 2 cut(s) 418, 459
BmrI ACTGGG 1 cut(s) 317
BmsI GCATC 3 cut(s) 22, 715, 783
BmuI ACTGGG 1 cut(s) 317
BoxI GACNNNNGTC 1 cut(s) 487
BpiI GAAGAC 1 cut(s) 221
BpuEI CTTGAG 2 cut(s) 459, 692
Bsa29I ATCGAT 1 cut(s) 261
BsaAI YACGTR 2 cut(s) 144, 625
BsaBI GATNNNNATC 1 cut(s) 297
Bse1I ACTGG 1 cut(s) 312
Bse3DI GCAATG 1 cut(s) 17
Bse8I GATNNNNATC 1 cut(s) 297
BseCI ATCGAT 1 cut(s) 261
BseGI GGATG 4 cut(s) 13, 378, 575, 613
BseJI GATNNNNATC 1 cut(s) 297
BseMI GCAATG 1 cut(s) 17
BseMII CTCAG 2 cut(s) 144, 653
BseNI ACTGG 1 cut(s) 312
BseSI GKGCMC 1 cut(s) 338
BsgI GTGCAG 1 cut(s) 647
BshFI GGCC 1 cut(s) 273
BshNI GGYRCC 2 cut(s) 416, 457
BshVI ATCGAT 1 cut(s) 261
BsiHKAI GWGCWC 3 cut(s) 320, 338, 567
BsnI GGCC 1 cut(s) 273
Bsp1286I GDGCHC 3 cut(s) 320, 338, 567
Bsp143I GATC 1 cut(s) 451
BspACI CCGC 2 cut(s) 677, 680
BspANI GGCC 1 cut(s) 273
BspCNI CTCAG 2 cut(s) 143, 652
BspDI ATCGAT 1 cut(s) 261
BspLI GGNNCC 2 cut(s) 418, 459
BspT107I GGYRCC 2 cut(s) 416, 457
BspTI CTTAAG 1 cut(s) 805
BsrDI GCAATG 1 cut(s) 17
BsrI ACTGG 1 cut(s) 312
BssMI GATC 1 cut(s) 451
BstAFI CTTAAG 1 cut(s) 805
BstAPI GCANNNNNTGC 1 cut(s) 19
BstBAI YACGTR 2 cut(s) 144, 625
BstDEI CTNAG 3 cut(s) 130, 404, 639
BstF5I GGATG 4 cut(s) 13, 378, 575, 613
BstKTI GATC 1 cut(s) 454
BstMBI GATC 1 cut(s) 451
BstMWI GCNNNNNNNGC 9 cut(s) 10, 19, 508, 517, 592, 634, 676, 679, 703
BstNSI RCATGY 1 cut(s) 670
BstPAI GACNNNNGTC 1 cut(s) 487
BstSLI GKGCMC 1 cut(s) 338
BstSNI TACGTA 1 cut(s) 144
BstV2I GAAGAC 1 cut(s) 221
Bsu15I ATCGAT 1 cut(s) 261
BsuRI GGCC 1 cut(s) 273
BsuTUI ATCGAT 1 cut(s) 261
BtsCI GGATG 4 cut(s) 13, 378, 575, 613
BtsIMutI CAGTG 1 cut(s) 305
CaiI CAGNNNCTG 1 cut(s) 598
Cfr13I GGNCC 2 cut(s) 271, 482
ClaI ATCGAT 1 cut(s) 261
Csp6I GTAC 4 cut(s) 393, 417, 601, 643
CviAII CATG 1 cut(s) 667
CviQI GTAC 4 cut(s) 393, 417, 601, 643
DdeI CTNAG 3 cut(s) 130, 404, 639
DpnI GATC 1 cut(s) 453
DpnII GATC 1 cut(s) 451
DraIII CACNNNGTG 1 cut(s) 389
Ecl136II GAGCTC 1 cut(s) 318
Eco105I TACGTA 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 320
Eco47I GGWCC 1 cut(s) 482
Eco53kI GAGCTC 1 cut(s) 318
Eco57I CTGAAG 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 318
EcoO109I RGGNCCY 1 cut(s) 271
EcoRI GAATTC 2 cut(s) 25, 88
EcoT38I GRGCYC 1 cut(s) 320
FaeI CATG 1 cut(s) 670
FatI CATG 1 cut(s) 666
FauNDI CATATG 1 cut(s) 97
FbaI TGATCA 1 cut(s) 451
FokI GGATG 3 cut(s) 385, 562, 600
FriOI GRGCYC 1 cut(s) 320
HaeIII GGCC 1 cut(s) 273
Hin1II CATG 1 cut(s) 670
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 5 cut(s) 188, 208, 298, 377, 655
HpaI GTTAAC 1 cut(s) 67
HphI GGTGA 2 cut(s) 97, 483
Hpy166II GTNNAC 2 cut(s) 67, 336
Hpy188I TCNGA 3 cut(s) 133, 213, 426
Hpy188III TCNNGA 6 cut(s) 33, 149, 344, 709, 718, 743
Hpy8I GTNNAC 2 cut(s) 67, 336
Hpy99I CGWCG 1 cut(s) 547
HpyAV CCTTC 2 cut(s) 363, 669
HpyCH4IV ACGT 3 cut(s) 143, 545, 624
HpyCH4V TGCA 9 cut(s) 4, 221, 278, 336, 511, 550, 628, 670, 728
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 19, 508, 517, 592, 634, 676, 679, 703
HpyF3I CTNAG 3 cut(s) 130, 404, 639
HpySE526I ACGT 3 cut(s) 143, 545, 624
Hsp92II CATG 1 cut(s) 670
KpnI GGTACC 1 cut(s) 420
Ksp22I TGATCA 1 cut(s) 451
KspAI GTTAAC 1 cut(s) 67
Kzo9I GATC 1 cut(s) 451
LmnI GCTCC 1 cut(s) 323
LpnPI CCDG 8 cut(s) 18, 25, 261, 293, 338, 440, 462, 703
LweI GCATC 3 cut(s) 22, 715, 783
MaeII ACGT 3 cut(s) 143, 545, 624
MaeIII GTNAC 2 cut(s) 442, 489
MalI GATC 1 cut(s) 453
MboI GATC 1 cut(s) 451
MboII GAAGA 3 cut(s) 226, 420, 458
MhlI GDGCHC 3 cut(s) 320, 338, 567
MluCI AATT 4 cut(s) 25, 88, 761, 823
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 6 cut(s) 40, 127, 262, 471, 606, 648
MseI TTAA 2 cut(s) 66, 806
MspCI CTTAAG 1 cut(s) 805
MwoI GCNNNNNNNGC 9 cut(s) 10, 19, 508, 517, 592, 634, 676, 679, 703
NdeI CATATG 1 cut(s) 97
NdeII GATC 1 cut(s) 451
NlaIII CATG 1 cut(s) 670
NlaIV GGNNCC 2 cut(s) 418, 459
NmuCI GTSAC 2 cut(s) 442, 489
NspI RCATGY 1 cut(s) 670
PfeI GAWTC 4 cut(s) 188, 208, 298, 655
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Ppu21I YACGTR 2 cut(s) 144, 625
PshAI GACNNNNGTC 1 cut(s) 487
Psp124BI GAGCTC 1 cut(s) 320
PspN4I GGNNCC 2 cut(s) 418, 459
PspPI GGNCC 2 cut(s) 271, 482
PstNI CAGNNNCTG 1 cut(s) 598
RsaI GTAC 4 cut(s) 394, 418, 602, 644
RsaNI GTAC 4 cut(s) 393, 417, 601, 643
SacI GAGCTC 1 cut(s) 320
SaqAI TTAA 2 cut(s) 66, 806
Sau3AI GATC 1 cut(s) 451
Sau96I GGNCC 2 cut(s) 271, 482
SchI GAGTC 1 cut(s) 386
SduI GDGCHC 3 cut(s) 320, 338, 567
SfaNI GCATC 3 cut(s) 22, 715, 783
SinI GGWCC 1 cut(s) 482
SmlI CTYRAG 3 cut(s) 474, 707, 805
SmoI CTYRAG 3 cut(s) 474, 707, 805
SnaBI TACGTA 1 cut(s) 144
Sse9I AATT 4 cut(s) 25, 88, 761, 823
SsiI CCGC 2 cut(s) 677, 680
SspI AATATT 1 cut(s) 793
SstI GAGCTC 1 cut(s) 320
TaiI ACGT 3 cut(s) 146, 548, 627
TaqI TCGA 1 cut(s) 261
TasI AATT 4 cut(s) 25, 88, 761, 823
TauI GCSGC 2 cut(s) 679, 682
TfiI GAWTC 4 cut(s) 188, 208, 298, 655
Tru1I TTAA 2 cut(s) 66, 806
Tru9I TTAA 2 cut(s) 66, 806
TscAI CASTG 1 cut(s) 312
TseFI GTSAC 2 cut(s) 442, 489
Tsp45I GTSAC 2 cut(s) 442, 489
TspDTI ATGAA 4 cut(s) 210, 425, 458, 602
TspRI CASTG 1 cut(s) 312
Vha464I CTTAAG 1 cut(s) 805
VneI GTGCAC 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 482
XapI RAATTY 2 cut(s) 25, 88
XceI RCATGY 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.