Rw5G021890

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000885
Physical Location & Seq
Reverse (-)
29812257 .. 29814626
2370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G021890.1

Sequence Viewer

Length: 786 bp
ATGAATTTGAGCTCAAGGAGGCTTCCTCCGATGGACTACCACCACCGCAAGGAGACTCGGCCTTCCTCTCCGCCACTCTCACCGGCTTCTTCATCCTCAGACACCCCAATTAACACCGTCCCCGGACAAATCATAGATTGCCTCAGAATCGGAATCGCCACGTTCGGACCAGCAAGAGAATTTTCATCCCTTTCCGAGCTTCCCCGATATATTCCACGTGGAGTCCTTCTGGGTAATCTTGACATACGGAAGGATCCAGTTGGACCAGTGAAAGCTTCAATTTCGGCCGACGGGCGAGGTATCATATTTGAAAGAATTGGAGCACCAGCTTATATATACGTTGTGACTAAACGTTTGGCACGGTCGGAGAGGGCATTGGATGAGCTCTCGGAGGTTGTAGAAGAAAAAACAGTGCTGCCAATATTCCACGGTGTTGATCCCTCCGATGTACGACATCAAACGGGAAGTTTTGCACAAGCCTTTGCAGACCATGAGATAAAGTACAAGGATGACCCACAAAAAGTTAAAAGGTGGAGAGCTGCTTTAACCAAACTGGGAAATCTTGCAGGCTGGCATTCAAAGGGCTGTCACGAAACAAAACTCATTCAAGACGTTGTTGATGAGATACGGTGGAAATTGTATTCCCCTTTTTCAAAACCTGCCATTAGAAATCTGCCATCAACACCTTCTAGGTCTTCTTCTTATCGACGAAAGCATGATGTCTTTCTAAGCTTTAGAGGGGCGGATACCCGCTGGCATTTATGGGCCATCTACATGCTGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

261

Amino Acids

29.47

Weight (kDa)

9.74

Isoelectric Point (pI)

57.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 112 - 215 2.3e-18 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 667
AciI CCGC 4 cut(s) 46, 71, 743, 751
AclI AACGTT 1 cut(s) 352
AclWI GGATC 3 cut(s) 248, 261, 431
AcoI YGGCCR 1 cut(s) 285
AcsI RAATTY 2 cut(s) 4, 179
AcvI CACGTG 1 cut(s) 218
AfaI GTAC 2 cut(s) 450, 503
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 5 cut(s) 279, 311, 579, 608, 654
AluBI AGCT 7 cut(s) 12, 199, 275, 329, 385, 539, 732
AluI AGCT 7 cut(s) 12, 199, 275, 329, 385, 539, 732
Alw21I GWGCWC 3 cut(s) 14, 325, 387
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 3 cut(s) 248, 261, 431
AoxI GGCC 3 cut(s) 59, 285, 765
ApeKI GCWGC 3 cut(s) 415, 539, 778
ApoI RAATTY 2 cut(s) 4, 179
ArsI GACNNNNNNTTYG 2 cut(s) 337, 369
AspS9I GGNCC 3 cut(s) 167, 263, 765
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 1 cut(s) 72
AvaII GGWCC 2 cut(s) 167, 263
BamHI GGATCC 1 cut(s) 253
BanII GRGCYC 2 cut(s) 14, 387
BbrPI CACGTG 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 687
Bbv12I GWGCWC 3 cut(s) 14, 325, 387
BbvI GCAGC 3 cut(s) 402, 526, 765
BccI CCATC 3 cut(s) 25, 685, 776
BciVI GTATCC 1 cut(s) 739
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 47
BfaI CTAG 1 cut(s) 690
BfuAI ACCTGC 1 cut(s) 667
BfuI GTATCC 1 cut(s) 739
BisI GCNGC 3 cut(s) 416, 540, 779
BlsI GCNGC 3 cut(s) 417, 541, 780
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 2 cut(s) 167, 263
BmgT120I GGNCC 3 cut(s) 167, 263, 765
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 1 cut(s) 123
BmrI ACTGGG 1 cut(s) 563
BmuI ACTGGG 1 cut(s) 563
BpiI GAAGAC 1 cut(s) 687
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpuMI CCSGG 1 cut(s) 123
BsaAI YACGTR 1 cut(s) 218
BsaJI CCNNGG 2 cut(s) 121, 427
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 3 cut(s) 257, 266, 558
BseDI CCNNGG 2 cut(s) 121, 427
BseGI GGATG 4 cut(s) 92, 185, 385, 514
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMII CTCAG 2 cut(s) 111, 157
BseNI ACTGG 3 cut(s) 257, 266, 558
BseX3I CGGCCG 1 cut(s) 285
BseXI GCAGC 3 cut(s) 402, 526, 765
Bsh1285I CGRYCG 2 cut(s) 288, 365
BshFI GGCC 3 cut(s) 61, 287, 767
BsiEI CGRYCG 2 cut(s) 288, 365
BsiHKAI GWGCWC 3 cut(s) 14, 325, 387
BsiSI CCGG 2 cut(s) 83, 123
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 49
BsmAI GTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 104
BsmI GAATGC 1 cut(s) 574
BsnI GGCC 3 cut(s) 61, 287, 767
Bsp1286I GDGCHC 3 cut(s) 14, 325, 387
Bsp143I GATC 2 cut(s) 253, 436
BspACI CCGC 4 cut(s) 46, 71, 743, 751
BspANI GGCC 3 cut(s) 61, 287, 767
BspCNI CTCAG 2 cut(s) 110, 156
BspLI GGNNCC 1 cut(s) 255
BspMI ACCTGC 1 cut(s) 667
BspPI GGATC 3 cut(s) 248, 261, 431
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 3 cut(s) 257, 266, 558
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 2 cut(s) 121, 427
BssMI GATC 2 cut(s) 253, 436
Bst4CI ACNGT 5 cut(s) 118, 363, 412, 431, 630
BstBAI YACGTR 1 cut(s) 218
BstC8I GCNNGC 3 cut(s) 568, 572, 755
BstDEI CTNAG 3 cut(s) 97, 143, 728
BstDSI CCRYGG 1 cut(s) 427
BstF5I GGATG 4 cut(s) 92, 185, 385, 514
BstKTI GATC 2 cut(s) 256, 439
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 2 cut(s) 253, 436
BstMCI CGRYCG 2 cut(s) 288, 365
BstNSI RCATGY 1 cut(s) 778
BstSCI CCNGG 1 cut(s) 121
BstV1I GCAGC 3 cut(s) 402, 526, 765
BstV2I GAAGAC 1 cut(s) 687
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BstZI CGGCCG 1 cut(s) 285
BsuI GTATCC 1 cut(s) 739
BsuRI GGCC 3 cut(s) 61, 287, 767
BtgI CCRYGG 1 cut(s) 427
BtsCI GGATG 4 cut(s) 92, 185, 385, 514
BtsIMutI CAGTG 2 cut(s) 273, 417
BveI ACCTGC 1 cut(s) 667
Cac8I GCNNGC 3 cut(s) 568, 572, 755
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 3 cut(s) 167, 263, 765
Csp6I GTAC 2 cut(s) 449, 502
CviAII CATG 3 cut(s) 491, 716, 775
CviQI GTAC 2 cut(s) 449, 502
DdeI CTNAG 3 cut(s) 97, 143, 728
DpnI GATC 2 cut(s) 255, 438
DpnII GATC 2 cut(s) 253, 436
EaeI YGGCCR 1 cut(s) 285
EagI CGGCCG 1 cut(s) 285
EciI GGCGGA 2 cut(s) 60, 758
Ecl136II GAGCTC 2 cut(s) 12, 385
EclXI CGGCCG 1 cut(s) 285
Eco24I GRGCYC 2 cut(s) 14, 387
Eco47I GGWCC 2 cut(s) 167, 263
Eco52I CGGCCG 1 cut(s) 285
Eco53kI GAGCTC 2 cut(s) 12, 385
Eco72I CACGTG 1 cut(s) 218
EcoICRI GAGCTC 2 cut(s) 12, 385
EcoT38I GRGCYC 2 cut(s) 14, 387
FaeI CATG 3 cut(s) 494, 719, 778
FaqI GGGAC 1 cut(s) 104
FatI CATG 3 cut(s) 490, 715, 774
FauI CCCGC 1 cut(s) 758
Fnu4HI GCNGC 3 cut(s) 416, 540, 779
FokI GGATG 4 cut(s) 79, 172, 392, 521
FriOI GRGCYC 2 cut(s) 14, 387
Fsp4HI GCNGC 3 cut(s) 416, 540, 779
FspBI CTAG 1 cut(s) 690
GluI GCNGC 3 cut(s) 416, 540, 779
HaeIII GGCC 3 cut(s) 61, 287, 767
HapII CCGG 2 cut(s) 83, 123
Hin1II CATG 3 cut(s) 494, 719, 778
HindIII AAGCTT 2 cut(s) 273, 730
HinfI GANTC 4 cut(s) 55, 147, 153, 222
HpaII CCGG 2 cut(s) 83, 123
HphI GGTGA 1 cut(s) 72
Hpy188I TCNGA 9 cut(s) 30, 100, 146, 152, 167, 196, 367, 391, 445
Hpy188III TCNNGA 3 cut(s) 239, 590, 608
Hpy99I CGWCG 2 cut(s) 293, 711
HpyAV CCTTC 4 cut(s) 72, 236, 244, 696
HpyCH4III ACNGT 5 cut(s) 118, 363, 412, 431, 630
HpyCH4IV ACGT 5 cut(s) 161, 217, 339, 352, 612
HpyCH4V TGCA 4 cut(s) 473, 485, 566, 781
HpyF3I CTNAG 3 cut(s) 97, 143, 728
HpySE526I ACGT 5 cut(s) 161, 217, 339, 352, 612
Hsp92II CATG 3 cut(s) 494, 719, 778
Kzo9I GATC 2 cut(s) 253, 436
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 3 cut(s) 402, 526, 765
MaeI CTAG 1 cut(s) 690
MaeII ACGT 5 cut(s) 161, 217, 339, 352, 612
MaeIII GTNAC 2 cut(s) 343, 587
MalI GATC 2 cut(s) 255, 438
MboI GATC 2 cut(s) 253, 436
MboII GAAGA 4 cut(s) 81, 413, 687, 690
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 3 cut(s) 14, 325, 387
MluCI AATT 6 cut(s) 4, 108, 179, 279, 315, 635
MlyI GAGTC 2 cut(s) 49, 231
MmeI TCCRAC 2 cut(s) 241, 345
MseI TTAA 4 cut(s) 111, 525, 545, 784
MslI CAYNNNNRTG 1 cut(s) 773
MspA1I CMGCKG 1 cut(s) 753
MspI CCGG 2 cut(s) 83, 123
MspR9I CCNGG 1 cut(s) 123
Mva1269I GAATGC 1 cut(s) 574
NciI CCSGG 1 cut(s) 123
NdeII GATC 2 cut(s) 253, 436
NlaIII CATG 3 cut(s) 494, 719, 778
NlaIV GGNNCC 1 cut(s) 255
NmeAIII GCCGAG 1 cut(s) 37
NmuCI GTSAC 2 cut(s) 343, 587
NspI RCATGY 1 cut(s) 778
PctI GAATGC 1 cut(s) 574
PfeI GAWTC 2 cut(s) 147, 153
PkrI GCNGC 3 cut(s) 417, 541, 780
PleI GAGTC 2 cut(s) 49, 230
PmaCI CACGTG 1 cut(s) 218
PmlI CACGTG 1 cut(s) 218
PpsI GAGTC 2 cut(s) 49, 230
Ppu21I YACGTR 1 cut(s) 218
Psp124BI GAGCTC 2 cut(s) 14, 387
Psp1406I AACGTT 1 cut(s) 352
PspCI CACGTG 1 cut(s) 218
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 3 cut(s) 167, 263, 765
PsuI RGATCY 1 cut(s) 253
RsaI GTAC 2 cut(s) 450, 503
RsaNI GTAC 2 cut(s) 449, 502
RseI CAYNNNNRTG 1 cut(s) 773
SacI GAGCTC 2 cut(s) 14, 387
SaqAI TTAA 4 cut(s) 111, 525, 545, 784
SatI GCNGC 3 cut(s) 416, 540, 779
Sau3AI GATC 2 cut(s) 253, 436
Sau96I GGNCC 3 cut(s) 167, 263, 765
SchI GAGTC 2 cut(s) 49, 231
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 3 cut(s) 14, 325, 387
SinI GGWCC 2 cut(s) 167, 263
SmiMI CAYNNNNRTG 1 cut(s) 773
SmlI CTYRAG 1 cut(s) 13
SmoI CTYRAG 1 cut(s) 13
Sse9I AATT 6 cut(s) 4, 108, 179, 279, 315, 635
SsiI CCGC 4 cut(s) 46, 71, 743, 751
SspI AATATT 1 cut(s) 423
SspMI CTAG 1 cut(s) 690
SstI GAGCTC 2 cut(s) 14, 387
StyD4I CCNGG 1 cut(s) 121
TaaI ACNGT 5 cut(s) 118, 363, 412, 431, 630
TaiI ACGT 5 cut(s) 164, 220, 342, 355, 615
TaqI TCGA 1 cut(s) 706
TasI AATT 6 cut(s) 4, 108, 179, 279, 315, 635
TatI WGTACW 1 cut(s) 501
TfiI GAWTC 2 cut(s) 147, 153
Tru1I TTAA 4 cut(s) 111, 525, 545, 784
Tru9I TTAA 4 cut(s) 111, 525, 545, 784
TscAI CASTG 2 cut(s) 273, 417
TseFI GTSAC 2 cut(s) 343, 587
TseI GCWGC 3 cut(s) 415, 539, 778
Tsp45I GTSAC 2 cut(s) 343, 587
TspDTI ATGAA 3 cut(s) 17, 81, 174
TspGWI ACGGA 1 cut(s) 262
TspRI CASTG 2 cut(s) 273, 417
VpaK11BI GGWCC 2 cut(s) 167, 263
XapI RAATTY 2 cut(s) 4, 179
XceI RCATGY 1 cut(s) 778
XspI CTAG 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.