Rmu_sc0001493.1_g000069

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001493.1
OG0000885
Physical Location & Seq
Forward (+)
254835 .. 256523
1689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001493.1_g000069.1.cds

Sequence Viewer

Length: 819 bp
atggccataccagtacaacgaccccagaaactccaatgtcacaagatgccaaaaataaatacacgtctacgttttccggagattcatattggcttctttttgtcttccgataacaatcatatatatgtcgtcactcaaagtttggcacggtcggagacggcgttggaggacctcgcggagcttcttgaagccgcgaacaatagtagtaatgtacaaaaaacagtgctgccaatattctacggtgttgagccatccgatgtgcgacatcaaacagcaagttttgcacaagccttcgccaactatgagaaaaagtacaaggatgatccacaaaaagtagaaagatggagagctgctttaaggaaactggcaaatctcgctggctgggattcaaagcactggcctgaagcaaaactcattcgagaagttgttgatgagatatggaggaaattgtattcctctttttcaaaacctgccattaaaaatctggcatcaacaccttcttggtcttcttcttgtcgacagaagcatgatgtatttctaagctttagagggaaggatacccgcttgaattttttggatcatgcattaattcagtgcagaatttccacttttagagatgacaaagatcttgttaaaggaatgtccattcgcaaaccaatcgaaaaagcaattgagggatcaaaggtttgggttgtcattgtttcaccaaactatgcttcttcaaaatggtgcttggatgagcttttgaagattcttgaatgcagaaaagcatctaaagtggggaagaagaaaaaaagtttgatgaaggtgttgggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

272

Amino Acids

31.23

Weight (kDa)

9.74

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 478
AccI GTMKAC 2 cut(s) 67, 517
AccII CGCG 2 cut(s) 176, 194
AccIII TCCGGA 1 cut(s) 76
AciI CCGC 3 cut(s) 176, 192, 562
AclWI GGATC 3 cut(s) 317, 585, 685
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 568, 600
AcuI CTGAAG 1 cut(s) 423
AfaI GTAC 3 cut(s) 15, 213, 314
AflIII ACRYGT 1 cut(s) 62
AgsI TTSAA 7 cut(s) 188, 390, 465, 568, 723, 748, 758
AjiI CACGTC 1 cut(s) 65
AjuI GAANNNNNNNTTGG 2 cut(s) 700, 732
AluBI AGCT 4 cut(s) 181, 350, 543, 742
AluI AGCT 4 cut(s) 181, 350, 543, 742
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 3 cut(s) 317, 585, 685
Aor13HI TCCGGA 1 cut(s) 76
AoxI GGCC 2 cut(s) 3, 398
ApeKI GCWGC 2 cut(s) 226, 350
ApoI RAATTY 2 cut(s) 568, 600
AseI ATTAAT 1 cut(s) 587
AspS9I GGNCC 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 696
AvaII GGWCC 1 cut(s) 169
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 96, 498
BbvI GCAGC 2 cut(s) 213, 337
BccI CCATC 2 cut(s) 259, 336
BceAI ACGGC 1 cut(s) 174
BcgI CGANNNNNNTGC 2 cut(s) 640, 674
BciVI GTATCC 1 cut(s) 550
BcoDI GTCTC 1 cut(s) 149
BfuAI ACCTGC 1 cut(s) 478
BfuI GTATCC 1 cut(s) 550
BglII AGATCT 1 cut(s) 625
BisI GCNGC 3 cut(s) 192, 227, 351
BlsI GCNGC 3 cut(s) 193, 228, 352
Bme18I GGWCC 1 cut(s) 169
BmgBI CACGTC 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 169
BmsI GCATC 3 cut(s) 36, 497, 779
BpiI GAAGAC 2 cut(s) 96, 498
BsaBI GATNNNNATC 1 cut(s) 114
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bse1I ACTGG 3 cut(s) 11, 369, 401
Bse8I GATNNNNATC 1 cut(s) 114
BseAI TCCGGA 1 cut(s) 76
BseGI GGATG 3 cut(s) 251, 325, 742
BseJI GATNNNNATC 1 cut(s) 114
BseNI ACTGG 3 cut(s) 11, 369, 401
BseXI GCAGC 2 cut(s) 213, 337
BseYI CCCAGC 1 cut(s) 381
BsgI GTGCAG 1 cut(s) 616
Bsh1236I CGCG 2 cut(s) 176, 194
Bsh1285I CGRYCG 1 cut(s) 152
BshFI GGCC 2 cut(s) 5, 400
BsiEI CGRYCG 1 cut(s) 152
BsiSI CCGG 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 149
BsmBI CGTCTC 1 cut(s) 149
BsmI GAATGC 1 cut(s) 764
BsnI GGCC 2 cut(s) 5, 400
Bsp13I TCCGGA 1 cut(s) 76
Bsp1407I TGTACA 1 cut(s) 211
Bsp143I GATC 4 cut(s) 322, 577, 625, 677
BspACI CCGC 3 cut(s) 176, 192, 562
BspANI GGCC 2 cut(s) 5, 400
BspEI TCCGGA 1 cut(s) 76
BspFNI CGCG 2 cut(s) 176, 194
BspMI ACCTGC 1 cut(s) 478
BspPI GGATC 3 cut(s) 317, 585, 685
BsrGI TGTACA 1 cut(s) 211
BsrI ACTGG 3 cut(s) 11, 369, 401
BssMI GATC 4 cut(s) 322, 577, 625, 677
Bst4CI ACNGT 3 cut(s) 150, 223, 242
BstAPI GCANNNNNTGC 1 cut(s) 281
BstAUI TGTACA 1 cut(s) 211
BstC8I GCNNGC 1 cut(s) 379
BstDEI CTNAG 1 cut(s) 539
BstF5I GGATG 3 cut(s) 251, 325, 742
BstFNI CGCG 2 cut(s) 176, 194
BstKTI GATC 4 cut(s) 325, 580, 628, 680
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 4 cut(s) 322, 577, 625, 677
BstMCI CGRYCG 1 cut(s) 152
BstMWI GCNNNNNNNGC 2 cut(s) 281, 374
BstUI CGCG 2 cut(s) 176, 194
BstV1I GCAGC 2 cut(s) 213, 337
BstV2I GAAGAC 2 cut(s) 96, 498
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BsuI GTATCC 1 cut(s) 550
BsuRI GGCC 2 cut(s) 5, 400
BtrI CACGTC 1 cut(s) 65
BtsCI GGATG 3 cut(s) 251, 325, 742
BtsIMutI CAGTG 3 cut(s) 228, 394, 599
BveI ACCTGC 1 cut(s) 478
Cac8I GCNNGC 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 169
Csp6I GTAC 3 cut(s) 14, 212, 313
CviAII CATG 2 cut(s) 527, 581
CviQI GTAC 3 cut(s) 14, 212, 313
DdeI CTNAG 1 cut(s) 539
DpnI GATC 4 cut(s) 324, 579, 627, 679
DpnII GATC 4 cut(s) 322, 577, 625, 677
EaeI YGGCCR 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 423
EcoO109I RGGNCCY 1 cut(s) 169
EcoT22I ATGCAT 1 cut(s) 586
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 2 cut(s) 530, 584
FatI CATG 2 cut(s) 526, 580
FauI CCCGC 1 cut(s) 569
FblI GTMKAC 2 cut(s) 67, 517
Fnu4HI GCNGC 3 cut(s) 192, 227, 351
FokI GGATG 3 cut(s) 238, 332, 749
Fsp4HI GCNGC 3 cut(s) 192, 227, 351
GluI GCNGC 3 cut(s) 192, 227, 351
GsaI CCCAGC 1 cut(s) 385
HaeIII GGCC 2 cut(s) 5, 400
HapII CCGG 1 cut(s) 77
Hin1II CATG 2 cut(s) 530, 584
HincII GTYRAC 1 cut(s) 518
HindII GTYRAC 1 cut(s) 518
HindIII AAGCTT 1 cut(s) 541
HinfI GANTC 3 cut(s) 82, 386, 751
HpaII CCGG 1 cut(s) 77
HphI GGTGA 1 cut(s) 696
Hpy166II GTNNAC 2 cut(s) 68, 518
Hpy188I TCNGA 3 cut(s) 109, 154, 256
Hpy188III TCNNGA 4 cut(s) 77, 185, 419, 755
Hpy8I GTNNAC 2 cut(s) 68, 518
HpyAV CCTTC 4 cut(s) 301, 507, 547, 799
HpyCH4III ACNGT 3 cut(s) 150, 223, 242
HpyCH4IV ACGT 2 cut(s) 64, 70
HpyCH4V TGCA 4 cut(s) 284, 584, 597, 762
HpyF10VI GCNNNNNNNGC 2 cut(s) 281, 374
HpyF3I CTNAG 1 cut(s) 539
HpySE526I ACGT 2 cut(s) 64, 70
Hsp92II CATG 2 cut(s) 530, 584
Kpn2I TCCGGA 1 cut(s) 76
Kzo9I GATC 4 cut(s) 322, 577, 625, 677
LmnI GCTCC 1 cut(s) 178
Lsp1109I GCAGC 2 cut(s) 213, 337
LweI GCATC 3 cut(s) 36, 497, 779
MaeII ACGT 2 cut(s) 64, 70
MaeIII GTNAC 2 cut(s) 38, 130
MalI GATC 4 cut(s) 324, 579, 627, 679
MboI GATC 4 cut(s) 322, 577, 625, 677
MboII GAAGA 7 cut(s) 96, 498, 501, 711, 760, 796, 799
MfeI CAATTG 1 cut(s) 669
MflI RGATCY 1 cut(s) 625
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 446, 568, 588, 600, 669
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 132, 144
MnlI CCTC 6 cut(s) 160, 182, 435, 466, 542, 667
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 586
MroI TCCGGA 1 cut(s) 76
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 356, 477, 587, 633, 817
MslI CAYNNNNRTG 1 cut(s) 123
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 77
MunI CAATTG 1 cut(s) 669
Mva1269I GAATGC 1 cut(s) 764
MvnI CGCG 2 cut(s) 176, 194
MwoI GCNNNNNNNGC 2 cut(s) 281, 374
NdeII GATC 4 cut(s) 322, 577, 625, 677
NlaIII CATG 2 cut(s) 530, 584
NmuCI GTSAC 2 cut(s) 38, 130
NsiI ATGCAT 1 cut(s) 586
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PctI GAATGC 1 cut(s) 764
PfeI GAWTC 3 cut(s) 82, 386, 751
PkrI GCNGC 3 cut(s) 193, 228, 352
PpuMI RGGWCCY 1 cut(s) 169
PshBI ATTAAT 1 cut(s) 587
Psp5II RGGWCCY 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 381
PspPI GGNCC 1 cut(s) 169
PspPPI RGGWCCY 1 cut(s) 169
PsuI RGATCY 1 cut(s) 625
RsaI GTAC 3 cut(s) 15, 213, 314
RsaNI GTAC 3 cut(s) 14, 212, 313
RseI CAYNNNNRTG 1 cut(s) 123
SalI GTCGAC 1 cut(s) 516
SaqAI TTAA 5 cut(s) 356, 477, 587, 633, 817
SatI GCNGC 3 cut(s) 192, 227, 351
Sau3AI GATC 4 cut(s) 322, 577, 625, 677
Sau96I GGNCC 1 cut(s) 169
SfaNI GCATC 3 cut(s) 36, 497, 779
SinI GGWCC 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 123
Sse9I AATT 5 cut(s) 446, 568, 588, 600, 669
SsiI CCGC 3 cut(s) 176, 192, 562
SspI AATATT 1 cut(s) 234
TaaI ACNGT 3 cut(s) 150, 223, 242
TaiI ACGT 2 cut(s) 67, 73
TaqI TCGA 3 cut(s) 418, 517, 660
TasI AATT 5 cut(s) 446, 568, 588, 600, 669
TatI WGTACW 3 cut(s) 13, 211, 312
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 3 cut(s) 82, 386, 751
Tru1I TTAA 5 cut(s) 356, 477, 587, 633, 817
Tru9I TTAA 5 cut(s) 356, 477, 587, 633, 817
TscAI CASTG 3 cut(s) 228, 401, 599
TseFI GTSAC 2 cut(s) 38, 130
TseI GCWGC 2 cut(s) 226, 350
Tsp45I GTSAC 2 cut(s) 38, 130
TspDTI ATGAA 2 cut(s) 74, 818
TspRI CASTG 3 cut(s) 228, 401, 599
VpaK11BI GGWCC 1 cut(s) 169
VspI ATTAAT 1 cut(s) 587
XapI RAATTY 2 cut(s) 568, 600
XcmI CCANNNNNNNNNTGG 1 cut(s) 481
XmiI GTMKAC 2 cut(s) 67, 517
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.