MD08G1022000.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000885
Physical Location & Seq
Reverse (-)
1598146 .. 1604418
6273 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1022000.v1.1.491

Sequence Viewer

Length: 393 bp
ATGAAAGAGAAAAAGAGAAATTTTCCCATTTTTTATGGCGTGGATCCTTCTGATGTACGCCATCAACGAGGGAGTTTTGGGGAGGCCTTTGCGGAACATGAAGTAAATTATCGAGAACACATGGAGGAGGTAAATGAGTGGAGAAAGTCGTTAAAAAGGGTGGCTAATCTCGCTGGGTGGAATTCAAAGGATTGTAGGTATGATACAGAGCTTATCCAAGGAATCGTCAATACACTATGGGAGGGAGTGCATCCTACGTTGTCAATGTTGGATTCCTCAGAGAGGTTTGTCGGAATCGATTCTAAACGGAAGGAAATAGATTTGCTTTTAGAAACAGATGCAAACGATGTTCGCTTTATAGGGATATGGGGGATGGGTGGACTTTCTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

131

Amino Acids

15.05

Weight (kDa)

5.58

Isoelectric Point (pI)

30.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 9 - 97 5.2e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 92
AclWI GGATC 2 cut(s) 38, 51
AcsI RAATTY 2 cut(s) 19, 181
AfaI GTAC 1 cut(s) 57
AfiI CCNNNNNNNGG 1 cut(s) 282
AgsI TTSAA 1 cut(s) 186
AluBI AGCT 1 cut(s) 211
AluI AGCT 1 cut(s) 211
AlwI GGATC 2 cut(s) 38, 51
AoxI GGCC 1 cut(s) 84
ApoI RAATTY 2 cut(s) 19, 181
Asp700I GAANNNNTTC 1 cut(s) 298
BamHI GGATCC 1 cut(s) 43
BccI CCATC 2 cut(s) 69, 367
BmiI GGNNCC 1 cut(s) 45
BmsI GCATC 2 cut(s) 259, 328
Bsa29I ATCGAT 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 217
Bsc4I CCNNNNNNNGG 1 cut(s) 282
BseCI ATCGAT 1 cut(s) 297
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 2 cut(s) 250, 378
BseLI CCNNNNNNNGG 1 cut(s) 282
BseMII CTCAG 1 cut(s) 291
BseRI GAGGAG 1 cut(s) 140
BseYI CCCAGC 1 cut(s) 173
BshFI GGCC 1 cut(s) 86
BshVI ATCGAT 1 cut(s) 297
BslI CCNNNNNNNGG 1 cut(s) 282
BsnI GGCC 1 cut(s) 86
Bsp143I GATC 1 cut(s) 43
BspACI CCGC 1 cut(s) 92
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 1 cut(s) 290
BspDI ATCGAT 1 cut(s) 297
BspLI GGNNCC 1 cut(s) 45
BspPI GGATC 2 cut(s) 38, 51
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 1 cut(s) 43
BssT1I CCWWGG 1 cut(s) 217
BstDEI CTNAG 1 cut(s) 277
BstENI CCTNNNNNAGG 1 cut(s) 280
BstF5I GGATG 2 cut(s) 250, 378
BstKTI GATC 1 cut(s) 46
BstMBI GATC 1 cut(s) 43
BstMWI GCNNNNNNNGC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 43
BstYI RGATCY 1 cut(s) 43
Bsu15I ATCGAT 1 cut(s) 297
BsuRI GGCC 1 cut(s) 86
BsuTUI ATCGAT 1 cut(s) 297
BtsCI GGATG 2 cut(s) 250, 378
ClaI ATCGAT 1 cut(s) 297
Csp6I GTAC 1 cut(s) 56
CviAII CATG 2 cut(s) 98, 121
CviJI RGCY 3 cut(s) 86, 164, 211
CviKI_1 RGCY 3 cut(s) 86, 164, 211
CviQI GTAC 1 cut(s) 56
DdeI CTNAG 1 cut(s) 277
DpnI GATC 1 cut(s) 45
DpnII GATC 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 217
Eco147I AGGCCT 1 cut(s) 86
EcoNI CCTNNNNNAGG 1 cut(s) 280
EcoRI GAATTC 1 cut(s) 181
EcoT14I CCWWGG 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 2 cut(s) 101, 124
FaiI YATR 7 cut(s) 36, 99, 122, 201, 238, 359, 367
FatI CATG 2 cut(s) 97, 120
FokI GGATG 2 cut(s) 237, 385
GsaI CCCAGC 1 cut(s) 177
HaeIII GGCC 1 cut(s) 86
Hin1II CATG 2 cut(s) 101, 124
HinfI GANTC 4 cut(s) 222, 272, 294, 299
Hpy166II GTNNAC 1 cut(s) 380
Hpy188I TCNGA 3 cut(s) 52, 280, 293
Hpy188III TCNNGA 1 cut(s) 113
Hpy8I GTNNAC 1 cut(s) 380
HpyAV CCTTC 2 cut(s) 57, 304
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 2 cut(s) 250, 341
HpyF10VI GCNNNNNNNGC 1 cut(s) 170
HpyF3I CTNAG 1 cut(s) 277
HpySE526I ACGT 1 cut(s) 257
Hsp92II CATG 2 cut(s) 101, 124
Kzo9I GATC 1 cut(s) 43
LpnPI CCDG 1 cut(s) 159
LweI GCATC 2 cut(s) 259, 328
MaeII ACGT 1 cut(s) 257
MalI GATC 1 cut(s) 45
MboI GATC 1 cut(s) 43
MflI RGATCY 1 cut(s) 43
MluCI AATT 3 cut(s) 19, 106, 181
MmeI TCCRAC 2 cut(s) 249, 271
MnlI CCTC 7 cut(s) 62, 76, 118, 121, 235, 276, 286
MroXI GAANNNNTTC 1 cut(s) 298
MseI TTAA 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 170
NdeII GATC 1 cut(s) 43
NlaIII CATG 2 cut(s) 101, 124
NlaIV GGNNCC 1 cut(s) 45
PceI AGGCCT 1 cut(s) 86
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 298
PfeI GAWTC 4 cut(s) 222, 272, 294, 299
PspFI CCCAGC 1 cut(s) 173
PspN4I GGNNCC 1 cut(s) 45
PsuI RGATCY 1 cut(s) 43
RsaI GTAC 1 cut(s) 57
RsaNI GTAC 1 cut(s) 56
SaqAI TTAA 1 cut(s) 152
Sau3AI GATC 1 cut(s) 43
SetI ASST 5 cut(s) 132, 200, 213, 260, 287
SfaNI GCATC 2 cut(s) 259, 328
SgeI CNNG 8 cut(s) 52, 80, 110, 125, 133, 182, 186, 230
Sse9I AATT 3 cut(s) 19, 106, 181
SseBI AGGCCT 1 cut(s) 86
SsiI CCGC 1 cut(s) 92
StuI AGGCCT 1 cut(s) 86
StyI CCWWGG 1 cut(s) 217
TaiI ACGT 1 cut(s) 260
TaqI TCGA 2 cut(s) 112, 297
TasI AATT 3 cut(s) 19, 106, 181
TfiI GAWTC 4 cut(s) 222, 272, 294, 299
Tru1I TTAA 1 cut(s) 152
Tru9I TTAA 1 cut(s) 152
TspDTI ATGAA 2 cut(s) 17, 114
TspGWI ACGGA 1 cut(s) 322
XagI CCTNNNNNAGG 1 cut(s) 280
XapI RAATTY 2 cut(s) 19, 181
XmnI GAANNNNTTC 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.