Rroxscaffold_1G00045140

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000885
Physical Location & Seq
Reverse (-)
64166086 .. 64175796
9711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00045140.1

Sequence Viewer

Length: 1206 bp
ATGGGCCATCTACATGCTGCATTAATTCACCGCGGAATTTCCACCTTTAGAGATGACAAAGATCTTGAGAAAGGAAAGTCCATTCCCAAAGAACTCGACAAAGCAATTGAGGGATCAAAGGTTTGTGTTGTCATCGTTTCACCAGACTATGCTTCTTCAAAATGGTGCTTGAATGAGCTTTTGAAGATTCTTGAATGCGCTTTTGAAGATTCTTCCTTACCGCAACATCATGAAAAAGCCCACAATTTGGAGAGCAGATACAAAATAAATAAGTGGAAGGCTAGTTTGCAGAACAAGGAAGACATCTCTAATCATAACGAAAGGAAAATGGTTAAGCTTAGCCTCACGGTTAATTATGATGGAGAATGGGCCAGTCCTGTTTACAGTGGTGATAAAACAAAAGAAATAGTGATTTCAGATGACAGTACTCCTGATGAGCTTCTGGATCAAATACATAGTTTTGTTGGGGTGGATCCAAAACAAAATGAGAGCAATACTGATGATAACCAAAGAATTTTTATTGGTAAGAGCCTTTCTTCCAGTACGGGCTTCAAGAATCCACTGCGGAATGCTTGTGAAAGAAGAATGGCTGAAGTTCTAGTTTTGGTGAATTATGACGGGGAGTGGGTGAATTCTACATACGTTGGTGGTCAAACGAAAGGAATAGTGATTTCAGAGGATATTACGTATCAAGGACTTGTGGAGAGAGTGTATGGTGTTGTCGGAGTTGATCCACATGAATATAAGCTGATATTGAAGGCCAGATATGAATCAAAGTTTCCTACTCAACCCGTGGAGATAATCAATGATGATGACCTTGGATTCTTCATCAAAGAGAACCTTTCACGGGATATGAGCTCCCGAATTCCTTTGTGCACGACTCTTGAACGACGGTCATTTCCTTATGGAAGGGTGGAGTCATCAGAGAACAGCAATACGGCACATATTAACAGATTTGGGACCTATCACAGTTACTTTCATGGTTTGACACCTGATCCAGTGCCTTATCTGCAGCAGCAGCAGCGGCAGCCTTTCTACCAGCAGCAACAACCGTCTTACCGACAAACGTGGACATGGCAGCCTTACTACAAACCTCAGGAGCTGCAATGGGGATACCAACTGCCCCATCATTCCTACATGCCGCCACAATCTTCATGCCAACAGCCCCCCTCTGGTATCACTATCAGCCCGATATGTACCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

401

Amino Acids

46.01

Weight (kDa)

6.11

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 3 - 70 5.7e-21 TIR domain
TIR_2 PF13676 3 - 67 1.5e-11 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 247
AccII CGCG 1 cut(s) 33
AciI CCGC 6 cut(s) 31, 33, 221, 565, 1024, 1142
AclWI GGATC 6 cut(s) 121, 453, 467, 480, 725, 989
AcsI RAATTY 4 cut(s) 36, 513, 631, 864
AcuI CTGAAG 1 cut(s) 612
AfaI GTAC 3 cut(s) 427, 544, 1198
AfiI CCNNNNNNNGG 3 cut(s) 247, 847, 1172
AgsI TTSAA 8 cut(s) 159, 172, 184, 194, 206, 553, 757, 887
AluBI AGCT 6 cut(s) 178, 337, 439, 748, 858, 1102
AluI AGCT 6 cut(s) 178, 337, 439, 748, 858, 1102
Alw21I GWGCWC 2 cut(s) 860, 878
Alw44I GTGCAC 1 cut(s) 874
AlwI GGATC 6 cut(s) 121, 453, 467, 480, 725, 989
AlwNI CAGNNNCTG 1 cut(s) 1102
AoxI GGCC 3 cut(s) 4, 369, 759
ApaLI GTGCAC 1 cut(s) 874
ApeKI GCWGC 9 cut(s) 17, 1012, 1015, 1018, 1021, 1027, 1042, 1078, 1102
ApoI RAATTY 4 cut(s) 36, 513, 631, 864
AseI ATTAAT 1 cut(s) 23
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 3 cut(s) 4, 369, 960
AsuHPI GGTGA 5 cut(s) 20, 132, 401, 619, 640
AvaII GGWCC 1 cut(s) 960
AxyI CCTNAGG 1 cut(s) 1095
BaeGI GKGCMC 1 cut(s) 878
BamHI GGATCC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 860
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 2 cut(s) 860, 878
BbvI GCAGC 9 cut(s) 4, 1024, 1027, 1030, 1033, 1039, 1054, 1089, 1090
BccI CCATC 3 cut(s) 15, 353, 1134
BceAI ACGGC 1 cut(s) 954
BciVI GTATCC 1 cut(s) 1106
BfaI CTAG 2 cut(s) 282, 599
BfmI CTRYAG 1 cut(s) 1010
BfuI GTATCC 1 cut(s) 1106
BglII AGATCT 1 cut(s) 61
BlpI GCTNAGC 1 cut(s) 338
BmcAI AGTACT 1 cut(s) 427
Bme18I GGWCC 1 cut(s) 960
BmgT120I GGNCC 3 cut(s) 4, 369, 960
BmiI GGNNCC 2 cut(s) 474, 961
BpiI GAAGAC 1 cut(s) 306
Bpu1102I GCTNAGC 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 86
BsaAI YACGTR 1 cut(s) 687
BsaBI GATNNNNATC 1 cut(s) 769
BsaJI CCNNGG 3 cut(s) 31, 792, 817
BsaXI ACNNNNNCTCC 2 cut(s) 695, 725
Bsc4I CCNNNNNNNGG 3 cut(s) 247, 847, 1172
Bse1I ACTGG 3 cut(s) 372, 540, 998
Bse21I CCTNAGG 1 cut(s) 1095
Bse3DI GCAATG 1 cut(s) 1112
Bse8I GATNNNNATC 1 cut(s) 769
BseDI CCNNGG 3 cut(s) 31, 792, 817
BseJI GATNNNNATC 1 cut(s) 769
BseLI CCNNNNNNNGG 3 cut(s) 247, 847, 1172
BseMI GCAATG 1 cut(s) 1112
BseMII CTCAG 1 cut(s) 1109
BseNI ACTGG 3 cut(s) 372, 540, 998
BseSI GKGCMC 1 cut(s) 878
BseXI GCAGC 9 cut(s) 4, 1024, 1027, 1030, 1033, 1039, 1054, 1089, 1090
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 3 cut(s) 6, 371, 761
BsiHKAI GWGCWC 2 cut(s) 860, 878
BslFI GGGAC 1 cut(s) 973
BslI CCNNNNNNNGG 3 cut(s) 247, 847, 1172
BsmFI GGGAC 1 cut(s) 973
BsmI GAATGC 2 cut(s) 200, 574
BsnI GGCC 3 cut(s) 6, 371, 761
Bsp1286I GDGCHC 2 cut(s) 860, 878
Bsp143I GATC 6 cut(s) 61, 113, 445, 472, 730, 994
Bsp1720I GCTNAGC 1 cut(s) 338
BspACI CCGC 6 cut(s) 31, 33, 221, 565, 1024, 1142
BspANI GGCC 3 cut(s) 6, 371, 761
BspCNI CTCAG 1 cut(s) 1108
BspFNI CGCG 1 cut(s) 33
BspHI TCATGA 1 cut(s) 229
BspLI GGNNCC 2 cut(s) 474, 961
BspMAI CTGCAG 1 cut(s) 1014
BspPI GGATC 6 cut(s) 121, 453, 467, 480, 725, 989
BsrDI GCAATG 1 cut(s) 1112
BsrI ACTGG 3 cut(s) 372, 540, 998
BssECI CCNNGG 3 cut(s) 31, 792, 817
BssMI GATC 6 cut(s) 61, 113, 445, 472, 730, 994
BssT1I CCWWGG 1 cut(s) 817
Bst4CI ACNGT 6 cut(s) 349, 386, 425, 894, 971, 1053
BstBAI YACGTR 1 cut(s) 687
BstDEI CTNAG 2 cut(s) 338, 1095
BstDSI CCRYGG 2 cut(s) 31, 792
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 6 cut(s) 64, 116, 448, 475, 733, 997
BstMBI GATC 6 cut(s) 61, 113, 445, 472, 730, 994
BstMWI GCNNNNNNNGC 5 cut(s) 1009, 1018, 1021, 1024, 1027
BstNSI RCATGY 2 cut(s) 17, 1141
BstSFI CTRYAG 1 cut(s) 1010
BstSLI GKGCMC 1 cut(s) 878
BstSNI TACGTA 1 cut(s) 687
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 9 cut(s) 4, 1024, 1027, 1030, 1033, 1039, 1054, 1089, 1090
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 2 cut(s) 61, 472
BstYI RGATCY 2 cut(s) 61, 472
Bsu36I CCTNAGG 1 cut(s) 1095
BsuI GTATCC 1 cut(s) 1106
BsuRI GGCC 3 cut(s) 6, 371, 761
BtgI CCRYGG 2 cut(s) 31, 792
BtsI GCAGTG 1 cut(s) 560
BtsIMutI CAGTG 3 cut(s) 391, 560, 1005
CaiI CAGNNNCTG 1 cut(s) 1102
CciI TCATGA 1 cut(s) 229
CfoI GCGC 1 cut(s) 200
Cfr13I GGNCC 3 cut(s) 4, 369, 960
Cfr42I CCGCGG 1 cut(s) 34
Csp6I GTAC 3 cut(s) 426, 543, 1197
CviAII CATG 7 cut(s) 14, 230, 737, 980, 1074, 1138, 1155
CviQI GTAC 3 cut(s) 426, 543, 1197
DdeI CTNAG 2 cut(s) 338, 1095
DpnI GATC 6 cut(s) 63, 115, 447, 474, 732, 996
DpnII GATC 6 cut(s) 61, 113, 445, 472, 730, 994
Ecl136II GAGCTC 1 cut(s) 858
Eco105I TACGTA 1 cut(s) 687
Eco130I CCWWGG 1 cut(s) 817
Eco24I GRGCYC 1 cut(s) 860
Eco47I GGWCC 1 cut(s) 960
Eco53kI GAGCTC 1 cut(s) 858
Eco57I CTGAAG 1 cut(s) 612
Eco81I CCTNAGG 1 cut(s) 1095
EcoICRI GAGCTC 1 cut(s) 858
EcoO109I RGGNCCY 1 cut(s) 960
EcoRI GAATTC 2 cut(s) 631, 864
EcoT14I CCWWGG 1 cut(s) 817
EcoT38I GRGCYC 1 cut(s) 860
ErhI CCWWGG 1 cut(s) 817
FaeI CATG 7 cut(s) 17, 233, 740, 983, 1077, 1141, 1158
FalI AAGNNNNNCTT 2 cut(s) 825, 857
FaqI GGGAC 1 cut(s) 973
FatI CATG 7 cut(s) 13, 229, 736, 979, 1073, 1137, 1154
FriOI GRGCYC 1 cut(s) 860
FspBI CTAG 2 cut(s) 282, 599
GlaI GCGC 1 cut(s) 199
HaeIII GGCC 3 cut(s) 6, 371, 761
HhaI GCGC 1 cut(s) 200
Hin1II CATG 7 cut(s) 17, 233, 740, 983, 1077, 1141, 1158
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 7 cut(s) 187, 209, 556, 770, 822, 880, 917
HphI GGTGA 5 cut(s) 20, 132, 401, 619, 640
Hpy166II GTNNAC 3 cut(s) 382, 876, 1071
Hpy188I TCNGA 4 cut(s) 418, 676, 725, 925
Hpy188III TCNNGA 9 cut(s) 65, 191, 230, 431, 443, 553, 861, 884, 1097
Hpy8I GTNNAC 3 cut(s) 382, 876, 1071
Hpy99I CGWCG 1 cut(s) 894
HpyAV CCTTC 3 cut(s) 271, 751, 903
HpyCH4III ACNGT 6 cut(s) 349, 386, 425, 894, 971, 1053
HpyCH4IV ACGT 3 cut(s) 642, 686, 1067
HpyCH4V TGCA 5 cut(s) 20, 289, 876, 1012, 1105
HpyF10VI GCNNNNNNNGC 5 cut(s) 1009, 1018, 1021, 1024, 1027
HpyF3I CTNAG 2 cut(s) 338, 1095
HpySE526I ACGT 3 cut(s) 642, 686, 1067
Hsp92II CATG 7 cut(s) 17, 233, 740, 983, 1077, 1141, 1158
HspAI GCGC 1 cut(s) 198
KspI CCGCGG 1 cut(s) 34
Kzo9I GATC 6 cut(s) 61, 113, 445, 472, 730, 994
LmnI GCTCC 2 cut(s) 863, 1099
Lsp1109I GCAGC 9 cut(s) 4, 1024, 1027, 1030, 1033, 1039, 1054, 1089, 1090
MaeI CTAG 2 cut(s) 282, 599
MaeII ACGT 3 cut(s) 642, 686, 1067
MaeIII GTNAC 1 cut(s) 971
MalI GATC 6 cut(s) 63, 115, 447, 474, 732, 996
MboI GATC 6 cut(s) 61, 113, 445, 472, 730, 994
MboII GAAGA 9 cut(s) 147, 196, 204, 218, 311, 528, 594, 817, 1143
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 2 cut(s) 61, 472
MhlI GDGCHC 2 cut(s) 860, 878
MluCI AATT 9 cut(s) 24, 36, 105, 244, 352, 513, 610, 631, 864
MlyI GAGTC 2 cut(s) 874, 926
MmeI TCCRAC 1 cut(s) 703
MnlI CCTC 5 cut(s) 103, 353, 670, 1104, 1180
MseI TTAA 4 cut(s) 23, 333, 351, 948
MslI CAYNNNNRTG 1 cut(s) 12
MspA1I CMGCKG 2 cut(s) 33, 1024
MunI CAATTG 1 cut(s) 105
Mva1269I GAATGC 2 cut(s) 200, 574
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 5 cut(s) 1009, 1018, 1021, 1024, 1027
NdeII GATC 6 cut(s) 61, 113, 445, 472, 730, 994
NlaIII CATG 7 cut(s) 17, 233, 740, 983, 1077, 1141, 1158
NlaIV GGNNCC 2 cut(s) 474, 961
NspI RCATGY 2 cut(s) 17, 1141
PagI TCATGA 1 cut(s) 229
PctI GAATGC 2 cut(s) 200, 574
PfeI GAWTC 5 cut(s) 187, 209, 556, 770, 822
PflMI CCANNNNNTGG 1 cut(s) 247
PleI GAGTC 2 cut(s) 874, 925
PpsI GAGTC 2 cut(s) 874, 925
Ppu21I YACGTR 1 cut(s) 687
PpuMI RGGWCCY 1 cut(s) 960
PshBI ATTAAT 1 cut(s) 23
Psp124BI GAGCTC 1 cut(s) 860
Psp5II RGGWCCY 1 cut(s) 960
PspN4I GGNNCC 2 cut(s) 474, 961
PspPI GGNCC 3 cut(s) 4, 369, 960
PspPPI RGGWCCY 1 cut(s) 960
PstI CTGCAG 1 cut(s) 1014
PstNI CAGNNNCTG 1 cut(s) 1102
PsuI RGATCY 2 cut(s) 61, 472
RsaI GTAC 3 cut(s) 427, 544, 1198
RsaNI GTAC 3 cut(s) 426, 543, 1197
RseI CAYNNNNRTG 1 cut(s) 12
SacI GAGCTC 1 cut(s) 860
SacII CCGCGG 1 cut(s) 34
SaqAI TTAA 4 cut(s) 23, 333, 351, 948
Sau3AI GATC 6 cut(s) 61, 113, 445, 472, 730, 994
Sau96I GGNCC 3 cut(s) 4, 369, 960
ScaI AGTACT 1 cut(s) 427
SchI GAGTC 2 cut(s) 874, 926
SduI GDGCHC 2 cut(s) 860, 878
SfcI CTRYAG 1 cut(s) 1010
Sfr303I CCGCGG 1 cut(s) 34
SgrBI CCGCGG 1 cut(s) 34
SinI GGWCC 1 cut(s) 960
SmiMI CAYNNNNRTG 1 cut(s) 12
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
SnaBI TACGTA 1 cut(s) 687
Sse9I AATT 9 cut(s) 24, 36, 105, 244, 352, 513, 610, 631, 864
SsiI CCGC 6 cut(s) 31, 33, 221, 565, 1024, 1142
SspMI CTAG 2 cut(s) 282, 599
SstI GAGCTC 1 cut(s) 860
StyI CCWWGG 1 cut(s) 817
TaaI ACNGT 6 cut(s) 349, 386, 425, 894, 971, 1053
TaiI ACGT 3 cut(s) 645, 689, 1070
TaqI TCGA 1 cut(s) 96
TasI AATT 9 cut(s) 24, 36, 105, 244, 352, 513, 610, 631, 864
TatI WGTACW 1 cut(s) 425
TauI GCSGC 2 cut(s) 1027, 1144
TfiI GAWTC 5 cut(s) 187, 209, 556, 770, 822
Tru1I TTAA 4 cut(s) 23, 333, 351, 948
Tru9I TTAA 4 cut(s) 23, 333, 351, 948
TscAI CASTG 3 cut(s) 391, 567, 1005
TseI GCWGC 9 cut(s) 17, 1012, 1015, 1018, 1021, 1027, 1042, 1078, 1102
TspDTI ATGAA 6 cut(s) 246, 753, 783, 817, 968, 1143
TspRI CASTG 3 cut(s) 391, 567, 1005
Van91I CCANNNNNTGG 1 cut(s) 247
VneI GTGCAC 1 cut(s) 874
VpaK11BI GGWCC 1 cut(s) 960
VspI ATTAAT 1 cut(s) 23
XapI RAATTY 4 cut(s) 36, 513, 631, 864
XceI RCATGY 2 cut(s) 17, 1141
XspI CTAG 2 cut(s) 282, 599
ZrmI AGTACT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.