FvH4_3g15551

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000468
Physical Location & Seq
Forward (+)
9751643 .. 9752618
976 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g15551.t1

Sequence Viewer

Length: 468 bp
ATGCTAGACCCCGTACTTGATAATTGCACAGATAATAGATGGAAATGGTTTAAGAATTGCTTGGGAGCTTTAGATGGAACTTATGTTAGAGTACGTGTAGCTGCTACTGACAAGGCAAGATACCGAACTAGAAAGGCAGATATTGCAACAAATGTCTTGGCAGCTTGTTCGCGTGACATGCAGTTCACATTCGTGTTACCGGGATGGGAGGGTTCTGCATCCGACTCTAGAGTACTTCGTGATGTAGTAACTAGGCCAAATGGATTGAGAGTTCCCACCGTATTTGAGTTTGATCAGTTAGCAGATATGAAAAATGTTGATATGATTGACTCTACTGAAGCATCAGATCAATGGACGGCATGGAGGAATGAGAAAGCCTCTCAAATGTTTGATGATTGGACAAGACGTAGGAATGATAGAGGTGGTGGTAGAGGCAGTGCTAGGGGCGATGCTAGAGGCCATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

156

Amino Acids

17.59

Weight (kDa)

8.93

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 24 - 81 1.6e-07 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 357
AfaI GTAC 3 cut(s) 15, 93, 234
AflIII ACRYGT 1 cut(s) 94
AleI CACNNNNGTG 1 cut(s) 191
AloI GAACNNNNNNTCC 2 cut(s) 255, 287
AluBI AGCT 3 cut(s) 68, 101, 164
AluI AGCT 3 cut(s) 68, 101, 164
AoxI GGCC 2 cut(s) 254, 457
ApeKI GCWGC 2 cut(s) 101, 161
AsuC2I CCSGG 1 cut(s) 201
BbvI GCAGC 2 cut(s) 88, 173
BccI CCATC 3 cut(s) 33, 68, 198
BceAI ACGGC 1 cut(s) 372
BcgI CGANNNNNNTGC 2 cut(s) 150, 184
BclI TGATCA 1 cut(s) 292
BcnI CCSGG 1 cut(s) 201
BfaI CTAG 6 cut(s) 5, 129, 228, 252, 441, 453
BisI GCNGC 2 cut(s) 102, 162
BlsI GCNGC 2 cut(s) 103, 163
BmcAI AGTACT 1 cut(s) 234
Bme1390I CCNGG 1 cut(s) 201
BmrFI CCNGG 1 cut(s) 201
BmsI GCATC 3 cut(s) 227, 350, 439
BplI GAGNNNNNCTC 2 cut(s) 362, 394
BpuMI CCSGG 1 cut(s) 201
BsaAI YACGTR 1 cut(s) 95
BseGI GGATG 2 cut(s) 209, 218
BseXI GCAGC 2 cut(s) 88, 173
Bsh1236I CGCG 1 cut(s) 172
BshFI GGCC 2 cut(s) 256, 459
BsiSI CCGG 1 cut(s) 200
BsnI GGCC 2 cut(s) 256, 459
Bsp143I GATC 2 cut(s) 292, 346
BspANI GGCC 2 cut(s) 256, 459
BspFNI CGCG 1 cut(s) 172
BssMI GATC 2 cut(s) 292, 346
Bst4CI ACNGT 1 cut(s) 280
BstAPI GCANNNNNTGC 1 cut(s) 143
BstBAI YACGTR 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 465
BstF5I GGATG 2 cut(s) 209, 218
BstFNI CGCG 1 cut(s) 172
BstKTI GATC 2 cut(s) 295, 349
BstMBI GATC 2 cut(s) 292, 346
BstMWI GCNNNNNNNGC 2 cut(s) 143, 178
BstNSI RCATGY 1 cut(s) 181
BstSCI CCNGG 1 cut(s) 199
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 88, 173
BsuRI GGCC 2 cut(s) 256, 459
BtgZI GCGATG 1 cut(s) 462
BtsCI GGATG 2 cut(s) 209, 218
BtsI GCAGTG 1 cut(s) 442
BtsIMutI CAGTG 1 cut(s) 442
Csp6I GTAC 3 cut(s) 14, 92, 233
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 3 cut(s) 178, 360, 461
CviJI RGCY 6 cut(s) 68, 101, 164, 256, 377, 459
CviKI_1 RGCY 6 cut(s) 68, 101, 164, 256, 377, 459
CviQI GTAC 3 cut(s) 14, 92, 233
DdeI CTNAG 1 cut(s) 465
DpnI GATC 2 cut(s) 294, 348
DpnII GATC 2 cut(s) 292, 346
Eco57I CTGAAG 1 cut(s) 357
FaeI CATG 3 cut(s) 181, 363, 464
FaiI YATR 6 cut(s) 84, 179, 308, 323, 361, 462
FalI AAGNNNNNCTT 2 cut(s) 44, 76
FatI CATG 3 cut(s) 177, 359, 460
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 2 cut(s) 102, 162
FokI GGATG 2 cut(s) 205, 216
Fsp4HI GCNGC 2 cut(s) 102, 162
FspBI CTAG 6 cut(s) 5, 129, 228, 252, 441, 453
GluI GCNGC 2 cut(s) 102, 162
HaeIII GGCC 2 cut(s) 256, 459
HapII CCGG 1 cut(s) 200
Hin1II CATG 3 cut(s) 181, 363, 464
HinfI GANTC 2 cut(s) 224, 329
HpaII CCGG 1 cut(s) 200
Hpy166II GTNNAC 1 cut(s) 186
Hpy188I TCNGA 2 cut(s) 223, 346
Hpy188III TCNNGA 2 cut(s) 228, 239
Hpy8I GTNNAC 1 cut(s) 186
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 2 cut(s) 94, 406
HpyCH4V TGCA 4 cut(s) 27, 146, 181, 218
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 178
HpyF3I CTNAG 1 cut(s) 465
HpySE526I ACGT 2 cut(s) 94, 406
Hsp92II CATG 3 cut(s) 181, 363, 464
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 2 cut(s) 292, 346
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 1 cut(s) 213
Lsp1109I GCAGC 2 cut(s) 88, 173
LweI GCATC 3 cut(s) 227, 350, 439
MaeI CTAG 6 cut(s) 5, 129, 228, 252, 441, 453
MaeII ACGT 2 cut(s) 94, 406
MaeIII GTNAC 3 cut(s) 173, 195, 247
MalI GATC 2 cut(s) 294, 348
MboI GATC 2 cut(s) 292, 346
MluCI AATT 2 cut(s) 22, 55
MlyI GAGTC 2 cut(s) 218, 323
MmeI TCCRAC 1 cut(s) 246
MnlI CCTC 6 cut(s) 202, 357, 388, 413, 425, 449
MseI TTAA 1 cut(s) 51
MslI CAYNNNNRTG 1 cut(s) 191
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 201
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 2 cut(s) 143, 178
NciI CCSGG 1 cut(s) 201
NdeII GATC 2 cut(s) 292, 346
NlaIII CATG 3 cut(s) 181, 363, 464
NmuCI GTSAC 1 cut(s) 173
NspI RCATGY 1 cut(s) 181
OliI CACNNNNGTG 1 cut(s) 191
PkrI GCNGC 2 cut(s) 103, 163
PleI GAGTC 2 cut(s) 218, 323
PpsI GAGTC 2 cut(s) 218, 323
Ppu21I YACGTR 1 cut(s) 95
RsaI GTAC 3 cut(s) 15, 93, 234
RsaNI GTAC 3 cut(s) 14, 92, 233
RseI CAYNNNNRTG 1 cut(s) 191
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 2 cut(s) 102, 162
Sau3AI GATC 2 cut(s) 292, 346
ScaI AGTACT 1 cut(s) 234
SchI GAGTC 2 cut(s) 218, 323
ScrFI CCNGG 1 cut(s) 201
SetI ASST 6 cut(s) 70, 97, 103, 166, 409, 424
SfaNI GCATC 3 cut(s) 227, 350, 439
SmiMI CAYNNNNRTG 1 cut(s) 191
Sse9I AATT 2 cut(s) 22, 55
SspMI CTAG 6 cut(s) 5, 129, 228, 252, 441, 453
StyD4I CCNGG 1 cut(s) 199
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 2 cut(s) 97, 409
TasI AATT 2 cut(s) 22, 55
TatI WGTACW 1 cut(s) 232
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 1 cut(s) 442
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 2 cut(s) 101, 161
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 1 cut(s) 323
TspRI CASTG 1 cut(s) 442
XbaI TCTAGA 1 cut(s) 227
XceI RCATGY 1 cut(s) 181
XspI CTAG 6 cut(s) 5, 129, 228, 252, 441, 453
ZrmI AGTACT 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.