Rorug01G0237400
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000468
Physical Location & Seq
Reverse (-)
34205361 .. 34206183
823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0237400.1

Sequence Viewer

Length: 750 bp
ATGGAACCACAAAACCGACAACCAACAGAGGATGGAGGTAGTAACAAAGCAGCCGGGAGTAGTGCTAACATGCTTCGCAAGAAGAGCAGTACTAGGTGGATTCCCACTACAGATCAGATAAGAATCCTCAAGGAGCTTTTCTACAACAAGGGAGTTAGGTCCCCAACTATAGAGCAGATTCAGAGGATCTGTCTCCAGCTGAAATGGTATGGCAAGATCGAGGGCAAGAACGTTTTTTATTGGTTCGCGAACCAAAAGGCTCGGGAGAAGCAGAAGAAGAGGTCCACTTCAGATGTTCAAGTGCCCATGCAAAGATCAGGGCTTGTTGGTGATGACAATGTTAGCAATCGAAAACATAAGGATCAGTATATTAACTTTGGATCTTCTGCACCTGCTTCTGCTTCTTCCGCTGGTGTGATGATTGCTTTTAACGGGCAGATGGGGAACTATGGCGGTTATGGATCCATGAACATGGAGAAGAGTGCTAGGGATTGTTCAATCTCAACTGGAGGGGGAACTAGTTCTACTTTCTTTGACATGAATGTAGAACAAACTTTCATGGAACAAAGAAGAGAAGATCACCAGGAGATTGAAACCCTTTCACTGTTTCCCGTGCATGGTGAGGACATCTTTGGCAACATGAAGACTACTTCCGATGGAGGTGGCGGTCACGGTGGTGGCTCTCACACTTCCCTTGAGCTCAGCCTTTCCTGCGAGGGCAAAGCTGGAAAAAGTTTCCGGAGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

249

Amino Acids

27.48

Weight (kDa)

9.3

Isoelectric Point (pI)

55.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 31 - 91 2.1e-10 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 400
Acc36I ACCTGC 1 cut(s) 400
AccII CGCG 1 cut(s) 248
AccIII TCCGGA 1 cut(s) 738
AciI CCGC 4 cut(s) 408, 453, 666, 745
AclI AACGTT 1 cut(s) 231
AclWI GGATC 5 cut(s) 194, 369, 388, 456, 469
AcuI CTGAAG 1 cut(s) 273
AfaI GTAC 1 cut(s) 91
AfiI CCNNNNNNNGG 1 cut(s) 617
AgsI TTSAA 3 cut(s) 299, 498, 593
AhlI ACTAGT 1 cut(s) 518
AjnI CCWGG 1 cut(s) 582
AleI CACNNNNGTG 1 cut(s) 675
AluBI AGCT 4 cut(s) 136, 199, 700, 725
AluI AGCT 4 cut(s) 136, 199, 700, 725
Alw21I GWGCWC 1 cut(s) 702
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 5 cut(s) 194, 369, 388, 456, 469
Ama87I CYCGRG 1 cut(s) 261
Aor13HI TCCGGA 1 cut(s) 738
ApeKI GCWGC 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 520
AspS9I GGNCC 2 cut(s) 159, 282
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 3 cut(s) 341, 572, 632
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 2 cut(s) 159, 282
BaeGI GKGCMC 1 cut(s) 306
BamHI GGATCC 1 cut(s) 461
BanII GRGCYC 1 cut(s) 702
BbsI GAAGAC 1 cut(s) 650
Bbv12I GWGCWC 1 cut(s) 702
BbvI GCAGC 1 cut(s) 62
BccI CCATC 3 cut(s) 26, 433, 650
BciT130I CCWGG 1 cut(s) 584
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 197
BcuI ACTAGT 1 cut(s) 518
BfaI CTAG 3 cut(s) 93, 486, 519
BfmI CTRYAG 2 cut(s) 108, 168
BfuAI ACCTGC 1 cut(s) 400
BisI GCNGC 2 cut(s) 51, 745
BlpI GCTNAGC 1 cut(s) 701
BlsI GCNGC 2 cut(s) 52, 746
BmcAI AGTACT 1 cut(s) 91
Bme1390I CCNGG 2 cut(s) 55, 584
Bme18I GGWCC 2 cut(s) 159, 282
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 2 cut(s) 159, 282
BmiI GGNNCC 4 cut(s) 6, 161, 463, 743
BmrFI CCNGG 2 cut(s) 55, 584
BpiI GAAGAC 1 cut(s) 650
BpmI CTGGAG 2 cut(s) 179, 528
Bpu1102I GCTNAGC 1 cut(s) 701
BpuEI CTTGAG 2 cut(s) 113, 716
BpuMI CCSGG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 122
BsaWI WCCGGW 1 cut(s) 738
Bsc4I CCNNNNNNNGG 1 cut(s) 617
Bse1I ACTGG 1 cut(s) 511
Bse8I GATNNNNATC 1 cut(s) 122
BseAI TCCGGA 1 cut(s) 738
BseBI CCWGG 1 cut(s) 584
BseGI GGATG 1 cut(s) 37
BseJI GATNNNNATC 1 cut(s) 122
BseLI CCNNNNNNNGG 1 cut(s) 617
BseMII CTCAG 1 cut(s) 715
BseNI ACTGG 1 cut(s) 511
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 1 cut(s) 62
BsgI GTGCAG 1 cut(s) 372
Bsh1236I CGCG 1 cut(s) 248
BsiHKAI GWGCWC 1 cut(s) 702
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 2 cut(s) 54, 739
BslFI GGGAC 1 cut(s) 145
BslI CCNNNNNNNGG 1 cut(s) 617
BsmAI GTCTC 1 cut(s) 197
BsmFI GGGAC 1 cut(s) 145
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 2 cut(s) 306, 702
Bsp13I TCCGGA 1 cut(s) 738
Bsp143I GATC 8 cut(s) 112, 186, 216, 314, 361, 380, 461, 577
Bsp1720I GCTNAGC 1 cut(s) 701
Bsp68I TCGCGA 1 cut(s) 248
BspACI CCGC 4 cut(s) 408, 453, 666, 745
BspCNI CTCAG 1 cut(s) 714
BspEI TCCGGA 1 cut(s) 738
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 4 cut(s) 6, 161, 463, 743
BspMI ACCTGC 1 cut(s) 400
BspPI GGATC 5 cut(s) 194, 369, 388, 456, 469
BspQI GCTCTTC 1 cut(s) 77
BsrI ACTGG 1 cut(s) 511
BssMI GATC 8 cut(s) 112, 186, 216, 314, 361, 380, 461, 577
Bst2UI CCWGG 1 cut(s) 584
Bst4CI ACNGT 2 cut(s) 606, 674
Bst6I CTCTTC 4 cut(s) 77, 272, 473, 565
BstDEI CTNAG 1 cut(s) 701
BstF5I GGATG 1 cut(s) 37
BstFNI CGCG 1 cut(s) 248
BstKTI GATC 8 cut(s) 115, 189, 219, 317, 364, 383, 464, 580
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 8 cut(s) 112, 186, 216, 314, 361, 380, 461, 577
BstMWI GCNNNNNNNGC 3 cut(s) 84, 407, 711
BstNI CCWGG 1 cut(s) 584
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 2 cut(s) 53, 582
BstSFI CTRYAG 2 cut(s) 108, 168
BstSLI GKGCMC 1 cut(s) 306
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 1 cut(s) 62
BstV2I GAAGAC 1 cut(s) 650
BstX2I RGATCY 3 cut(s) 186, 380, 461
BstXI CCANNNNNNTGG 1 cut(s) 472
BstYI RGATCY 3 cut(s) 186, 380, 461
BtsCI GGATG 1 cut(s) 37
BtsIMutI CAGTG 1 cut(s) 602
BtuMI TCGCGA 1 cut(s) 248
BveI ACCTGC 1 cut(s) 400
Cfr13I GGNCC 2 cut(s) 159, 282
Csp6I GTAC 1 cut(s) 90
CviAII CATG 8 cut(s) 70, 307, 466, 472, 538, 559, 617, 640
CviQI GTAC 1 cut(s) 90
DdeI CTNAG 1 cut(s) 701
DpnI GATC 8 cut(s) 114, 188, 218, 316, 363, 382, 463, 579
DpnII GATC 8 cut(s) 112, 186, 216, 314, 361, 380, 461, 577
Eam1104I CTCTTC 4 cut(s) 77, 272, 473, 565
EarI CTCTTC 4 cut(s) 77, 272, 473, 565
Ecl136II GAGCTC 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 702
Eco47I GGWCC 2 cut(s) 159, 282
Eco53kI GAGCTC 1 cut(s) 700
Eco57I CTGAAG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 261
EcoICRI GAGCTC 1 cut(s) 700
EcoO109I RGGNCCY 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 582
EcoT38I GRGCYC 1 cut(s) 702
FaeI CATG 8 cut(s) 73, 310, 469, 475, 541, 562, 620, 643
FaqI GGGAC 1 cut(s) 145
FatI CATG 8 cut(s) 69, 306, 465, 471, 537, 558, 616, 639
Fnu4HI GCNGC 2 cut(s) 51, 745
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 1 cut(s) 702
Fsp4HI GCNGC 2 cut(s) 51, 745
FspBI CTAG 3 cut(s) 93, 486, 519
GluI GCNGC 2 cut(s) 51, 745
GsuI CTGGAG 2 cut(s) 179, 528
HapII CCGG 2 cut(s) 54, 739
Hin1II CATG 8 cut(s) 73, 310, 469, 475, 541, 562, 620, 643
HinfI GANTC 3 cut(s) 100, 123, 178
HpaII CCGG 2 cut(s) 54, 739
HphI GGTGA 3 cut(s) 341, 572, 632
Hpy166II GTNNAC 1 cut(s) 285
Hpy188I TCNGA 4 cut(s) 117, 183, 292, 655
Hpy188III TCNNGA 3 cut(s) 247, 263, 739
Hpy8I GTNNAC 1 cut(s) 285
HpyCH4III ACNGT 2 cut(s) 606, 674
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 3 cut(s) 310, 389, 616
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 407, 711
HpyF3I CTNAG 1 cut(s) 701
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 8 cut(s) 73, 310, 469, 475, 541, 562, 620, 643
Kpn2I TCCGGA 1 cut(s) 738
Kzo9I GATC 8 cut(s) 112, 186, 216, 314, 361, 380, 461, 577
LguI GCTCTTC 1 cut(s) 77
LmnI GCTCC 2 cut(s) 133, 741
Lsp1109I GCAGC 1 cut(s) 62
MaeI CTAG 3 cut(s) 93, 486, 519
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 2 cut(s) 41, 668
MalI GATC 8 cut(s) 114, 188, 218, 316, 363, 382, 463, 579
MboI GATC 8 cut(s) 112, 186, 216, 314, 361, 380, 461, 577
MboII GAAGA 9 cut(s) 94, 286, 289, 375, 396, 490, 582, 587, 655
MflI RGATCY 3 cut(s) 186, 380, 461
MhlI GDGCHC 2 cut(s) 306, 702
MroI TCCGGA 1 cut(s) 738
MroXI GAANNNNTTC 1 cut(s) 520
MseI TTAA 2 cut(s) 372, 429
MslI CAYNNNNRTG 2 cut(s) 470, 675
MspA1I CMGCKG 3 cut(s) 199, 410, 747
MspI CCGG 2 cut(s) 54, 739
MspR9I CCNGG 2 cut(s) 55, 584
MvaI CCWGG 1 cut(s) 584
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 3 cut(s) 84, 407, 711
NciI CCSGG 1 cut(s) 55
NdeII GATC 8 cut(s) 112, 186, 216, 314, 361, 380, 461, 577
NlaIII CATG 8 cut(s) 73, 310, 469, 475, 541, 562, 620, 643
NlaIV GGNNCC 4 cut(s) 6, 161, 463, 743
NmuCI GTSAC 1 cut(s) 668
NruI TCGCGA 1 cut(s) 248
NspI RCATGY 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 675
PaqCI CACCTGC 1 cut(s) 400
PciSI GCTCTTC 1 cut(s) 77
PdmI GAANNNNTTC 1 cut(s) 520
PfeI GAWTC 3 cut(s) 100, 123, 178
PkrI GCNGC 2 cut(s) 52, 746
PpuMI RGGWCCY 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 702
Psp1406I AACGTT 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 582
PspGI CCWGG 1 cut(s) 582
PspN4I GGNNCC 4 cut(s) 6, 161, 463, 743
PspPI GGNCC 2 cut(s) 159, 282
PspPPI RGGWCCY 1 cut(s) 159
PsrI GAACNNNNNNTAC 2 cut(s) 508, 540
PsuI RGATCY 3 cut(s) 186, 380, 461
PvuII CAGCTG 1 cut(s) 199
RruI TCGCGA 1 cut(s) 248
RsaI GTAC 1 cut(s) 91
RsaNI GTAC 1 cut(s) 90
RseI CAYNNNNRTG 2 cut(s) 470, 675
SacI GAGCTC 1 cut(s) 702
SapI GCTCTTC 1 cut(s) 77
SaqAI TTAA 2 cut(s) 372, 429
SatI GCNGC 2 cut(s) 51, 745
Sau3AI GATC 8 cut(s) 112, 186, 216, 314, 361, 380, 461, 577
Sau96I GGNCC 2 cut(s) 159, 282
ScaI AGTACT 1 cut(s) 91
ScrFI CCNGG 2 cut(s) 55, 584
SduI GDGCHC 2 cut(s) 306, 702
SfcI CTRYAG 2 cut(s) 108, 168
SinI GGWCC 2 cut(s) 159, 282
SmiMI CAYNNNNRTG 2 cut(s) 470, 675
SmlI CTYRAG 2 cut(s) 128, 695
SmoI CTYRAG 2 cut(s) 128, 695
SpeI ACTAGT 1 cut(s) 518
SsiI CCGC 4 cut(s) 408, 453, 666, 745
SspMI CTAG 3 cut(s) 93, 486, 519
SstI GAGCTC 1 cut(s) 702
StyD4I CCNGG 2 cut(s) 53, 582
TaaI ACNGT 2 cut(s) 606, 674
TaiI ACGT 1 cut(s) 234
TaqI TCGA 2 cut(s) 219, 349
TatI WGTACW 1 cut(s) 89
TauI GCSGC 1 cut(s) 747
TfiI GAWTC 3 cut(s) 100, 123, 178
Tru1I TTAA 2 cut(s) 372, 429
Tru9I TTAA 2 cut(s) 372, 429
TscAI CASTG 1 cut(s) 609
TseFI GTSAC 1 cut(s) 668
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 668
TspDTI ATGAA 4 cut(s) 482, 547, 554, 656
TspRI CASTG 1 cut(s) 609
VpaK11BI GGWCC 2 cut(s) 159, 282
XceI RCATGY 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 520
XspI CTAG 3 cut(s) 93, 486, 519
ZrmI AGTACT 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.