pycom10g02500

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000468
Physical Location & Seq
Forward (+)
2870940 .. 2872191
1252 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g02500.1

Sequence Viewer

Length: 633 bp
ATGGCACAAGTCTTTACTATGAATGCACAATTGTTGAGATATAGACAACAACAAAGGAGACAACGGGAAAGAGCTAGTTTAGAGCAGAAGACATTTGTTAGACTTCATGTTAGGAGAGAGGGAATAAATCGTATCACAGGATTGAGTGATACTAACTGTTTGTGGAAGTTGCGAATAGATAGGAATGCATTTGCAGTTTCATATGATTTACTACAAATTCGTGGTGGGTTGGTAGATGATGGTCATGTTACAATAGAGGAGCAAGGTTGCTTAGGAGCACTAGATGGAACATACATAGAGGTCACTGTGCCTGAGGTCGATAGACCAAGATATAGAACAAGGAAGGGTCATATAGCAACTAATGTGTTAGTTGTATGCACACATGACCTCAAATTCGTATATGTGTTATCCGGTTGGGAGGGATCAGCTACTGATTCGACAATTCTTGGTGACACTGTTACTAGATCTAATGGCCTCAAGGTCCCAACCGGTAAGATAGACAAAATGTATATGGCGGTTGATCCTGAGGAAAATGCAAGGCTTGCATTTGATGAATTGTCTATAGGGGAAAATTTACCAGAAGTATTAGCCTACATTGAAACCGTTGACTCAAGCCAAACATGGACTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

211

Amino Acids

23.83

Weight (kDa)

6.11

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 94 - 152 2.7e-08 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 515
AclWI GGATC 2 cut(s) 430, 515
AcsI RAATTY 3 cut(s) 216, 392, 571
AgeI ACCGGT 1 cut(s) 488
AgsI TTSAA 1 cut(s) 599
AluBI AGCT 2 cut(s) 74, 428
AluI AGCT 2 cut(s) 74, 428
Alw21I GWGCWC 1 cut(s) 280
Alw26I GTCTC 1 cut(s) 52
AlwI GGATC 2 cut(s) 430, 515
AlwNI CAGNNNCTG 1 cut(s) 431
AoxI GGCC 1 cut(s) 472
ApoI RAATTY 3 cut(s) 216, 392, 571
ArsI GACNNNNNNTTYG 2 cut(s) 377, 409
AsiGI ACCGGT 1 cut(s) 488
AspS9I GGNCC 1 cut(s) 481
AsuHPI GGTGA 1 cut(s) 461
AvaII GGWCC 1 cut(s) 481
AxyI CCTNAGG 2 cut(s) 312, 525
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 280
BccI CCATC 2 cut(s) 233, 278
BcoDI GTCTC 1 cut(s) 52
BfaI CTAG 3 cut(s) 75, 281, 462
BfmI CTRYAG 1 cut(s) 561
BglII AGATCT 1 cut(s) 464
Bme18I GGWCC 1 cut(s) 481
BmgT120I GGNCC 1 cut(s) 481
BmiI GGNNCC 1 cut(s) 483
BpiI GAAGAC 1 cut(s) 95
Bpu10I CCTNAGC 1 cut(s) 271
BpuEI CTTGAG 2 cut(s) 461, 595
BsaWI WCCGGW 2 cut(s) 410, 488
Bse118I RCCGGY 1 cut(s) 488
Bse21I CCTNAGG 2 cut(s) 312, 525
BseMII CTCAG 2 cut(s) 303, 516
BseRI GAGGAG 1 cut(s) 272
BshFI GGCC 1 cut(s) 474
BshTI ACCGGT 1 cut(s) 488
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 2 cut(s) 411, 489
BslFI GGGAC 1 cut(s) 467
BsmAI GTCTC 1 cut(s) 52
BsmFI GGGAC 1 cut(s) 467
BsmI GAATGC 2 cut(s) 28, 190
BsnI GGCC 1 cut(s) 474
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 3 cut(s) 422, 464, 520
BspACI CCGC 1 cut(s) 515
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 2 cut(s) 304, 517
BspLI GGNNCC 1 cut(s) 483
BspPI GGATC 2 cut(s) 430, 515
BsrFI RCCGGY 1 cut(s) 488
BssAI RCCGGY 1 cut(s) 488
BssMI GATC 3 cut(s) 422, 464, 520
Bst4CI ACNGT 4 cut(s) 158, 307, 457, 604
BstAPI GCANNNNNTGC 1 cut(s) 542
BstC8I GCNNGC 1 cut(s) 543
BstDEI CTNAG 3 cut(s) 271, 312, 525
BstKTI GATC 3 cut(s) 425, 467, 523
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 3 cut(s) 422, 464, 520
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstSFI CTRYAG 1 cut(s) 561
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 464
BstYI RGATCY 1 cut(s) 464
Bsu36I CCTNAGG 2 cut(s) 312, 525
BsuRI GGCC 1 cut(s) 474
BtsIMutI CAGTG 2 cut(s) 303, 453
Cac8I GCNNGC 1 cut(s) 543
CaiI CAGNNNCTG 1 cut(s) 431
Cfr10I RCCGGY 1 cut(s) 488
Cfr13I GGNCC 1 cut(s) 481
CspAI ACCGGT 1 cut(s) 488
CviAII CATG 4 cut(s) 107, 245, 383, 621
CviJI RGCY 6 cut(s) 74, 428, 474, 541, 590, 615
CviKI_1 RGCY 6 cut(s) 74, 428, 474, 541, 590, 615
DdeI CTNAG 3 cut(s) 271, 312, 525
DpnI GATC 3 cut(s) 424, 466, 522
DpnII GATC 3 cut(s) 422, 464, 520
Eco47I GGWCC 1 cut(s) 481
Eco81I CCTNAGG 2 cut(s) 312, 525
EcoO109I RGGNCCY 1 cut(s) 481
EcoT22I ATGCAT 1 cut(s) 190
FaeI CATG 4 cut(s) 110, 248, 386, 624
FaqI GGGAC 1 cut(s) 467
FatI CATG 4 cut(s) 106, 244, 382, 620
FauNDI CATATG 1 cut(s) 202
FspBI CTAG 3 cut(s) 75, 281, 462
HaeIII GGCC 1 cut(s) 474
HapII CCGG 2 cut(s) 411, 489
Hin1II CATG 4 cut(s) 110, 248, 386, 624
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HinfI GANTC 3 cut(s) 434, 608, 625
HpaII CCGG 2 cut(s) 411, 489
HphI GGTGA 1 cut(s) 461
Hpy166II GTNNAC 1 cut(s) 607
Hpy188III TCNNGA 1 cut(s) 524
Hpy8I GTNNAC 1 cut(s) 607
HpyAV CCTTC 1 cut(s) 337
HpyCH4III ACNGT 4 cut(s) 158, 307, 457, 604
HpyCH4V TGCA 6 cut(s) 26, 188, 194, 378, 536, 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpyF3I CTNAG 3 cut(s) 271, 312, 525
Hsp92II CATG 4 cut(s) 110, 248, 386, 624
Kzo9I GATC 3 cut(s) 422, 464, 520
LmnI GCTCC 2 cut(s) 259, 275
LpnPI CCDG 6 cut(s) 123, 324, 424, 502, 537, 591
MaeI CTAG 3 cut(s) 75, 281, 462
MaeIII GTNAC 4 cut(s) 247, 301, 449, 457
MalI GATC 3 cut(s) 424, 466, 522
MboI GATC 3 cut(s) 422, 464, 520
MboII GAAGA 1 cut(s) 100
MfeI CAATTG 1 cut(s) 29
MflI RGATCY 1 cut(s) 464
MhlI GDGCHC 1 cut(s) 280
MluCI AATT 6 cut(s) 29, 216, 392, 441, 554, 571
MlyI GAGTC 2 cut(s) 602, 619
MnlI CCTC 8 cut(s) 112, 250, 292, 307, 398, 412, 485, 520
Mph1103I ATGCAT 1 cut(s) 190
MspI CCGG 2 cut(s) 411, 489
MunI CAATTG 1 cut(s) 29
Mva1269I GAATGC 2 cut(s) 28, 190
MwoI GCNNNNNNNGC 1 cut(s) 542
NdeI CATATG 1 cut(s) 202
NdeII GATC 3 cut(s) 422, 464, 520
NlaIII CATG 4 cut(s) 110, 248, 386, 624
NlaIV GGNNCC 1 cut(s) 483
NmuCI GTSAC 2 cut(s) 301, 449
NsiI ATGCAT 1 cut(s) 190
PctI GAATGC 2 cut(s) 28, 190
PfeI GAWTC 1 cut(s) 434
PinAI ACCGGT 1 cut(s) 488
PleI GAGTC 2 cut(s) 602, 619
PpsI GAGTC 2 cut(s) 602, 619
PpuMI RGGWCCY 1 cut(s) 481
Psp5II RGGWCCY 1 cut(s) 481
PspN4I GGNNCC 1 cut(s) 483
PspPI GGNCC 1 cut(s) 481
PspPPI RGGWCCY 1 cut(s) 481
PstNI CAGNNNCTG 1 cut(s) 431
PsuI RGATCY 1 cut(s) 464
Sau3AI GATC 3 cut(s) 422, 464, 520
Sau96I GGNCC 1 cut(s) 481
SchI GAGTC 2 cut(s) 602, 619
SduI GDGCHC 1 cut(s) 280
SetI ASST 7 cut(s) 76, 268, 303, 318, 390, 430, 483
SfcI CTRYAG 1 cut(s) 561
SinI GGWCC 1 cut(s) 481
SmlI CTYRAG 2 cut(s) 476, 610
SmoI CTYRAG 2 cut(s) 476, 610
Sse9I AATT 6 cut(s) 29, 216, 392, 441, 554, 571
SsiI CCGC 1 cut(s) 515
SspMI CTAG 3 cut(s) 75, 281, 462
TaaI ACNGT 4 cut(s) 158, 307, 457, 604
TaqI TCGA 2 cut(s) 318, 437
TasI AATT 6 cut(s) 29, 216, 392, 441, 554, 571
TfiI GAWTC 1 cut(s) 434
TscAI CASTG 2 cut(s) 310, 460
TseFI GTSAC 2 cut(s) 301, 449
Tsp45I GTSAC 2 cut(s) 301, 449
TspDTI ATGAA 4 cut(s) 35, 95, 189, 567
TspRI CASTG 2 cut(s) 310, 460
VpaK11BI GGWCC 1 cut(s) 481
XapI RAATTY 3 cut(s) 216, 392, 571
XspI CTAG 3 cut(s) 75, 281, 462
Zsp2I ATGCAT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.