MD02G1173300.v1.1
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000468
Physical Location & Seq
Reverse (-)
15177946 .. 15179018
1073 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1173300.v1.1.491

Sequence Viewer

Length: 876 bp
ATGACTTTACAAATATTAGCACATCATACTAAGAATTGTAGTGTTGGCGGTAGATTTTATAGGTCGGGAGAGACTATAAGTAGGTATTTCAATAGCGTATTGCAAGGAATTTTGCGATTACAAGGTATCCTACTAAAAGTCCCCCAGCCTGTGCCTATTGATTCTACAGATCCTAGGTGGCGATGTTTTAAGAATTGCTTGGGAGCATTGGATGGAACACACATTGATGTGCATGTACCTGAAATTGACAAACCAAGATACCGAACAAGAAAGGGTCGAGTCGCAACTAATGTGTTAGGTGTGTGTTCAGGAGATATGCAGTTCATATATGTGTTTCCGGGGTGGGAGGGTTCCGCATCAGACTCTAGAGTGCTACATGATGCAATTAGTAGGCCTAATGGTTTTAAGGTACCAGCGGGTTATTATTACCTTGTAGATGGTGGTTATACAAATGGTGAAGGATTCCTTGCACCCTATAGAGGAATACCTTATCATTTATCTGAATGGGAGGGACGAACACCTTCTAATAAGGAAGAATATTTTAACATGAAGCATTCTAAGGCAAGGAATGTAATTGAACGCTGTTTTGGCTTGCTAAAAGGAAGGTGGTCGATACTAAGGAGTCCATCTTTCTATCCGATAAGGACACAAGGTCGAATAATTACCGCTTGTTGCCTACTACACAATCTTATTAGGCAAGAGATGTCTGTAGATCCAATGGAGAATTTGCCAATAATAGAAGATGGACAAAATACAGAAGAAGGTGAATATGTTGGTAGTGTTCAAACATCGGACCAGTGGACTGCAATAAGGAATGATATGGCTCAGGAAATGTATAATGAGTGGAGAGCAATTAGGAACCAGCAACCGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

292

Amino Acids

33.22

Weight (kDa)

8.62

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 1 - 38 3.6e-07 Domain of unknown function (DUF8040)
Plant_tran PF04827 60 - 232 2.5e-08 Plant transposon protein
DDE_Tnp_1 PF01609 66 - 226 4.7e-10 Transposase DDE domain
DDE_Tnp_4 PF13359 70 - 229 2.3e-29 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 409
AccB1I GGYRCC 1 cut(s) 409
AciI CCGC 4 cut(s) 48, 354, 416, 666
AclWI GGATC 2 cut(s) 164, 707
AcsI RAATTY 2 cut(s) 108, 724
AfaI GTAC 2 cut(s) 237, 411
AfiI CCNNNNNNNGG 1 cut(s) 479
AgsI TTSAA 3 cut(s) 91, 578, 785
AhdI GACNNNNNGTC 1 cut(s) 651
AjuI GAANNNNNNNTTGG 2 cut(s) 570, 602
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 2 cut(s) 164, 707
AoxI GGCC 1 cut(s) 392
ApoI RAATTY 2 cut(s) 108, 724
Asp700I GAANNNNTTC 1 cut(s) 520
Asp718I GGTACC 1 cut(s) 409
AspA2I CCTAGG 1 cut(s) 173
AspS9I GGNCC 1 cut(s) 793
AsuC2I CCSGG 1 cut(s) 339
AsuHPI GGTGA 2 cut(s) 467, 776
AvaII GGWCC 1 cut(s) 793
AvrII CCTAGG 1 cut(s) 173
BanI GGYRCC 1 cut(s) 409
BccI CCATC 4 cut(s) 206, 431, 634, 737
BciVI GTATCC 1 cut(s) 137
BcnI CCSGG 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 65
BfaI CTAG 3 cut(s) 174, 366, 874
BfmI CTRYAG 3 cut(s) 165, 475, 708
BfuI GTATCC 1 cut(s) 137
BlnI CCTAGG 1 cut(s) 173
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 1 cut(s) 793
BmeRI GACNNNNNGTC 1 cut(s) 651
BmgT120I GGNCC 1 cut(s) 793
BmiI GGNNCC 3 cut(s) 352, 411, 860
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 2 cut(s) 365, 370
Bpu10I CCTNAGC 1 cut(s) 825
BpuMI CCSGG 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 173, 338
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse1I ACTGG 1 cut(s) 796
BseDI CCNNGG 2 cut(s) 173, 338
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMII CTCAG 1 cut(s) 839
BseNI ACTGG 1 cut(s) 796
BseYI CCCAGC 1 cut(s) 144
BshFI GGCC 1 cut(s) 394
BshNI GGYRCC 1 cut(s) 409
BsiSI CCGG 1 cut(s) 338
BslFI GGGAC 2 cut(s) 125, 525
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 1 cut(s) 65
BsmFI GGGAC 2 cut(s) 125, 525
BsmI GAATGC 1 cut(s) 553
BsnI GGCC 1 cut(s) 394
Bsp143I GATC 2 cut(s) 169, 712
BspACI CCGC 4 cut(s) 48, 354, 416, 666
BspANI GGCC 1 cut(s) 394
BspCNI CTCAG 1 cut(s) 838
BspLI GGNNCC 3 cut(s) 352, 411, 860
BspPI GGATC 2 cut(s) 164, 707
BspT107I GGYRCC 1 cut(s) 409
BsrI ACTGG 1 cut(s) 796
BssECI CCNNGG 2 cut(s) 173, 338
BssMI GATC 2 cut(s) 169, 712
BssT1I CCWWGG 1 cut(s) 173
BstC8I GCNNGC 1 cut(s) 593
BstDEI CTNAG 4 cut(s) 30, 558, 617, 825
BstF5I GGATG 1 cut(s) 217
BstKTI GATC 2 cut(s) 172, 715
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 2 cut(s) 169, 712
BstMWI GCNNNNNNNGC 1 cut(s) 588
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 337
BstSFI CTRYAG 3 cut(s) 165, 475, 708
BstX2I RGATCY 2 cut(s) 169, 712
BstYI RGATCY 2 cut(s) 169, 712
BsuI GTATCC 1 cut(s) 137
BsuRI GGCC 1 cut(s) 394
BtgZI GCGATG 1 cut(s) 196
BtsCI GGATG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 803
Cac8I GCNNGC 1 cut(s) 593
Cfr13I GGNCC 1 cut(s) 793
Csp6I GTAC 2 cut(s) 236, 410
CviAII CATG 3 cut(s) 233, 377, 547
CviJI RGCY 4 cut(s) 148, 394, 591, 824
CviKI_1 RGCY 4 cut(s) 148, 394, 591, 824
CviQI GTAC 2 cut(s) 236, 410
DdeI CTNAG 4 cut(s) 30, 558, 617, 825
DpnI GATC 2 cut(s) 171, 714
DpnII GATC 2 cut(s) 169, 712
DriI GACNNNNNGTC 1 cut(s) 651
Eam1105I GACNNNNNGTC 1 cut(s) 651
Eco130I CCWWGG 1 cut(s) 173
Eco147I AGGCCT 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 793
EcoT14I CCWWGG 1 cut(s) 173
ErhI CCWWGG 1 cut(s) 173
FaeI CATG 3 cut(s) 236, 380, 550
FalI AAGNNNNNCTT 4 cut(s) 182, 214, 450, 482
FaqI GGGAC 2 cut(s) 125, 525
FatI CATG 3 cut(s) 232, 376, 546
FauI CCCGC 1 cut(s) 409
FokI GGATG 1 cut(s) 224
FspBI CTAG 3 cut(s) 174, 366, 874
GsaI CCCAGC 1 cut(s) 148
HaeIII GGCC 1 cut(s) 394
HapII CCGG 1 cut(s) 338
Hin1II CATG 3 cut(s) 236, 380, 550
HinfI GANTC 5 cut(s) 161, 279, 362, 462, 622
HpaII CCGG 1 cut(s) 338
HphI GGTGA 2 cut(s) 467, 776
Hpy166II GTNNAC 1 cut(s) 801
Hpy188I TCNGA 4 cut(s) 361, 502, 639, 793
Hpy188III TCNNGA 4 cut(s) 66, 309, 366, 827
Hpy8I GTNNAC 1 cut(s) 801
HpyAV CCTTC 4 cut(s) 452, 531, 597, 755
HpyCH4V TGCA 6 cut(s) 103, 232, 319, 383, 470, 806
HpyF10VI GCNNNNNNNGC 1 cut(s) 588
HpyF3I CTNAG 4 cut(s) 30, 558, 617, 825
Hsp92II CATG 3 cut(s) 236, 380, 550
KpnI GGTACC 1 cut(s) 413
Kzo9I GATC 2 cut(s) 169, 712
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 8 cut(s) 158, 162, 252, 294, 351, 426, 809, 812
LweI GCATC 2 cut(s) 365, 370
MaeI CTAG 3 cut(s) 174, 366, 874
MalI GATC 2 cut(s) 171, 714
MboI GATC 2 cut(s) 169, 712
MboII GAAGA 3 cut(s) 545, 752, 770
MflI RGATCY 2 cut(s) 169, 712
MluCI AATT 9 cut(s) 34, 108, 193, 243, 384, 573, 660, 724, 852
MlyI GAGTC 3 cut(s) 288, 356, 631
MnlI CCTC 3 cut(s) 340, 473, 502
MroXI GAANNNNTTC 1 cut(s) 520
MseI TTAA 3 cut(s) 189, 405, 543
MslI CAYNNNNRTG 3 cut(s) 225, 227, 329
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 1 cut(s) 338
MspR9I CCNGG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 553
MwoI GCNNNNNNNGC 1 cut(s) 588
NciI CCSGG 1 cut(s) 339
NdeII GATC 2 cut(s) 169, 712
NlaIII CATG 3 cut(s) 236, 380, 550
NlaIV GGNNCC 3 cut(s) 352, 411, 860
NspI RCATGY 1 cut(s) 236
PceI AGGCCT 1 cut(s) 394
PctI GAATGC 1 cut(s) 553
PdmI GAANNNNTTC 1 cut(s) 520
PfeI GAWTC 2 cut(s) 161, 462
PleI GAGTC 3 cut(s) 287, 356, 630
PpsI GAGTC 3 cut(s) 287, 356, 630
PspFI CCCAGC 1 cut(s) 144
PspN4I GGNNCC 3 cut(s) 352, 411, 860
PspPI GGNCC 1 cut(s) 793
PsuI RGATCY 2 cut(s) 169, 712
RsaI GTAC 2 cut(s) 237, 411
RsaNI GTAC 2 cut(s) 236, 410
RseI CAYNNNNRTG 3 cut(s) 225, 227, 329
SaqAI TTAA 3 cut(s) 189, 405, 543
Sau3AI GATC 2 cut(s) 169, 712
Sau96I GGNCC 1 cut(s) 793
SchI GAGTC 3 cut(s) 288, 356, 631
ScrFI CCNGG 1 cut(s) 339
SfaNI GCATC 2 cut(s) 365, 370
SfcI CTRYAG 3 cut(s) 165, 475, 708
SinI GGWCC 1 cut(s) 793
SmiMI CAYNNNNRTG 3 cut(s) 225, 227, 329
Sse9I AATT 9 cut(s) 34, 108, 193, 243, 384, 573, 660, 724, 852
SseBI AGGCCT 1 cut(s) 394
SsiI CCGC 4 cut(s) 48, 354, 416, 666
SspI AATATT 2 cut(s) 15, 539
SspMI CTAG 3 cut(s) 174, 366, 874
StuI AGGCCT 1 cut(s) 394
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 1 cut(s) 173
TaqI TCGA 3 cut(s) 277, 611, 655
TasI AATT 9 cut(s) 34, 108, 193, 243, 384, 573, 660, 724, 852
TfiI GAWTC 2 cut(s) 161, 462
Tru1I TTAA 3 cut(s) 189, 405, 543
Tru9I TTAA 3 cut(s) 189, 405, 543
TscAI CASTG 1 cut(s) 803
TspDTI ATGAA 2 cut(s) 313, 563
TspRI CASTG 1 cut(s) 803
VpaK11BI GGWCC 1 cut(s) 793
XapI RAATTY 2 cut(s) 108, 724
XbaI TCTAGA 1 cut(s) 365
XceI RCATGY 1 cut(s) 236
XmaJI CCTAGG 1 cut(s) 173
XmnI GAANNNNTTC 1 cut(s) 520
XspI CTAG 3 cut(s) 174, 366, 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.