Rmu_sc0000060.1_g000010
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000060.1
OG0000468
Physical Location & Seq
Reverse (-)
74540 .. 75384
845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000060.1_g000010.1.cds

Sequence Viewer

Length: 666 bp
atgtacctgcaattgacaagccaagataccgcacaaaaaaagggtgaagttgctacaaatgtgctagctgcttgttctcgtgataggcagttcatatttgtcttaccgggttgggagggatcggcatcggattctagagttcttcacgatgtagtgactaggccgaatggattaagagttcccactggttattattaccttgtagatggtggttacacaaatggtgagggatttcttgcaccatatagaggaacaaggtatcatttgtcggaatgggatgaaagaaatatgcctagggatcatgtggaatattttaacatgaagcattcgaaggcaaggaatgtcatagaacggtgttttggaatactaaaaggaaggagagagatatccattgatccgatggagtatgcagtatgtgaattagatacacaaaatggggaagaagatgatgtagttggcacggttgaagcgtctaatcaatggactacttggaggaatgaattagctttacaaatgcataatgaatggacgggaagaagggcttaccgggatgctggagatacaggggtttcaggtgctgctgcaagggctgcaagggatgcaagagctgcaagggacggaagagctggaagggacggaagagctgcaagggacggaagagtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

221

Amino Acids

24.88

Weight (kDa)

6.53

Isoelectric Point (pI)

28.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 15
AciI CCGC 1 cut(s) 30
AclWI GGATC 3 cut(s) 127, 306, 389
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 248
AgsI TTSAA 1 cut(s) 467
AjuI GAANNNNNNNTTGG 2 cut(s) 342, 374
AloI GAACNNNNNNTCC 2 cut(s) 162, 194
AluBI AGCT 5 cut(s) 68, 506, 608, 626, 644
AluI AGCT 5 cut(s) 68, 506, 608, 626, 644
AlwI GGATC 3 cut(s) 127, 306, 389
AlwNI CAGNNNCTG 1 cut(s) 578
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 6 cut(s) 68, 578, 581, 590, 608, 644
AspA2I CCTAGG 1 cut(s) 293
AsuC2I CCSGG 2 cut(s) 108, 548
AsuHPI GGTGA 2 cut(s) 56, 236
AsuII TTCGAA 1 cut(s) 329
AsuNHI GCTAGC 1 cut(s) 64
AvrII CCTAGG 1 cut(s) 293
BaeI ACNNNNGTAYC 2 cut(s) 552, 585
BauI CACGAG 1 cut(s) 78
BbvI GCAGC 6 cut(s) 55, 565, 568, 577, 595, 631
BccI CCATC 2 cut(s) 200, 394
BcnI CCSGG 2 cut(s) 108, 548
BfaI CTAG 4 cut(s) 65, 135, 159, 294
BfuAI ACCTGC 1 cut(s) 15
BisI GCNGC 6 cut(s) 69, 579, 582, 591, 609, 645
BlnI CCTAGG 1 cut(s) 293
BlsI GCNGC 6 cut(s) 70, 580, 583, 592, 610, 646
Bme1390I CCNGG 2 cut(s) 108, 548
BmrFI CCNGG 2 cut(s) 108, 548
BmsI GCATC 3 cut(s) 134, 541, 589
BmtI GCTAGC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 576
Bpu14I TTCGAA 1 cut(s) 329
BpuMI CCSGG 2 cut(s) 108, 548
BsaBI GATNNNNATC 1 cut(s) 124
BsaJI CCNNGG 1 cut(s) 293
Bsc4I CCNNNNNNNGG 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 190
Bse8I GATNNNNATC 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 293
BseGI GGATG 3 cut(s) 283, 556, 604
BseJI GATNNNNATC 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 248
BseNI ACTGG 1 cut(s) 190
BseXI GCAGC 6 cut(s) 55, 565, 568, 577, 595, 631
BshFI GGCC 1 cut(s) 163
BsiSI CCGG 2 cut(s) 107, 547
BslFI GGGAC 2 cut(s) 629, 647
BslI CCNNNNNNNGG 1 cut(s) 248
BsmFI GGGAC 2 cut(s) 629, 647
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 1 cut(s) 163
Bsp119I TTCGAA 1 cut(s) 329
Bsp143I GATC 3 cut(s) 119, 298, 394
BspACI CCGC 1 cut(s) 30
BspANI GGCC 1 cut(s) 163
BspMI ACCTGC 1 cut(s) 15
BspOI GCTAGC 1 cut(s) 68
BspPI GGATC 3 cut(s) 127, 306, 389
BspQI GCTCTTC 2 cut(s) 616, 634
BspT104I TTCGAA 1 cut(s) 329
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 3 cut(s) 119, 298, 394
BssSI CACGAG 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 293
Bst2BI CACGAG 1 cut(s) 78
Bst4CI ACNGT 2 cut(s) 354, 463
Bst6I CTCTTC 3 cut(s) 616, 634, 652
BstAPI GCANNNNNTGC 3 cut(s) 590, 599, 608
BstBI TTCGAA 1 cut(s) 329
BstC8I GCNNGC 1 cut(s) 66
BstF5I GGATG 3 cut(s) 283, 556, 604
BstKTI GATC 3 cut(s) 122, 301, 397
BstMBI GATC 3 cut(s) 119, 298, 394
BstMWI GCNNNNNNNGC 4 cut(s) 587, 590, 599, 608
BstSCI CCNGG 2 cut(s) 106, 546
BstV1I GCAGC 6 cut(s) 55, 565, 568, 577, 595, 631
BsuRI GGCC 1 cut(s) 163
BtsCI GGATG 3 cut(s) 283, 556, 604
BtsIMutI CAGTG 1 cut(s) 183
BveI ACCTGC 1 cut(s) 15
Cac8I GCNNGC 1 cut(s) 66
CaiI CAGNNNCTG 1 cut(s) 578
CseI GACGC 1 cut(s) 459
Csp6I GTAC 1 cut(s) 4
CviAII CATG 2 cut(s) 302, 319
CviJI RGCY 9 cut(s) 21, 68, 163, 506, 542, 590, 608, 626, 644
CviKI_1 RGCY 9 cut(s) 21, 68, 163, 506, 542, 590, 608, 626, 644
CviQI GTAC 1 cut(s) 4
DpnI GATC 3 cut(s) 121, 300, 396
DpnII GATC 3 cut(s) 119, 298, 394
Eam1104I CTCTTC 3 cut(s) 616, 634, 652
EarI CTCTTC 3 cut(s) 616, 634, 652
Eco130I CCWWGG 1 cut(s) 293
Eco32I GATATC 1 cut(s) 387
EcoRV GATATC 1 cut(s) 387
EcoT14I CCWWGG 1 cut(s) 293
EcoT22I ATGCAT 1 cut(s) 519
ErhI CCWWGG 1 cut(s) 293
FaeI CATG 2 cut(s) 305, 322
FalI AAGNNNNNCTT 2 cut(s) 526, 558
FaqI GGGAC 2 cut(s) 629, 647
FatI CATG 2 cut(s) 301, 318
Fnu4HI GCNGC 6 cut(s) 69, 579, 582, 591, 609, 645
FokI GGATG 3 cut(s) 290, 563, 611
Fsp4HI GCNGC 6 cut(s) 69, 579, 582, 591, 609, 645
FspBI CTAG 4 cut(s) 65, 135, 159, 294
GluI GCNGC 6 cut(s) 69, 579, 582, 591, 609, 645
GsuI CTGGAG 1 cut(s) 576
HaeIII GGCC 1 cut(s) 163
HapII CCGG 2 cut(s) 107, 547
HgaI GACGC 1 cut(s) 459
Hin1II CATG 2 cut(s) 305, 322
HinfI GANTC 1 cut(s) 131
HpaII CCGG 2 cut(s) 107, 547
HphI GGTGA 2 cut(s) 56, 236
Hpy188I TCNGA 3 cut(s) 130, 271, 399
Hpy188III TCNNGA 3 cut(s) 80, 135, 146
HpyAV CCTTC 4 cut(s) 325, 369, 531, 624
HpyCH4III ACNGT 2 cut(s) 354, 463
HpyCH4V TGCA 9 cut(s) 10, 239, 410, 517, 584, 593, 602, 611, 647
HpyF10VI GCNNNNNNNGC 4 cut(s) 587, 590, 599, 608
Hsp92II CATG 2 cut(s) 305, 322
Kzo9I GATC 3 cut(s) 119, 298, 394
LguI GCTCTTC 2 cut(s) 616, 634
LpnPI CCDG 8 cut(s) 20, 120, 171, 540, 549, 558, 560, 612
Lsp1109I GCAGC 6 cut(s) 55, 565, 568, 577, 595, 631
LweI GCATC 3 cut(s) 134, 541, 589
MaeI CTAG 4 cut(s) 65, 135, 159, 294
MaeIII GTNAC 2 cut(s) 154, 212
MalI GATC 3 cut(s) 121, 300, 396
MboI GATC 3 cut(s) 119, 298, 394
MboII GAAGA 6 cut(s) 134, 452, 455, 546, 633, 651
MfeI CAATTG 1 cut(s) 11
MluCI AATT 3 cut(s) 11, 419, 500
MmeI TCCRAC 1 cut(s) 249
MnlI CCTC 4 cut(s) 109, 220, 242, 486
Mph1103I ATGCAT 1 cut(s) 519
MseI TTAA 2 cut(s) 173, 315
MspI CCGG 2 cut(s) 107, 547
MspR9I CCNGG 2 cut(s) 108, 548
MunI CAATTG 1 cut(s) 11
Mva1269I GAATGC 1 cut(s) 325
MwoI GCNNNNNNNGC 4 cut(s) 587, 590, 599, 608
NciI CCSGG 2 cut(s) 108, 548
NdeII GATC 3 cut(s) 119, 298, 394
NheI GCTAGC 1 cut(s) 64
NlaIII CATG 2 cut(s) 305, 322
NmuCI GTSAC 1 cut(s) 154
NsiI ATGCAT 1 cut(s) 519
NspV TTCGAA 1 cut(s) 329
PciSI GCTCTTC 2 cut(s) 616, 634
PcsI WCGNNNNNNNCGW 1 cut(s) 467
PctI GAATGC 1 cut(s) 325
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 6 cut(s) 70, 580, 583, 592, 610, 646
PstNI CAGNNNCTG 1 cut(s) 578
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SapI GCTCTTC 2 cut(s) 616, 634
SaqAI TTAA 2 cut(s) 173, 315
SatI GCNGC 6 cut(s) 69, 579, 582, 591, 609, 645
Sau3AI GATC 3 cut(s) 119, 298, 394
ScrFI CCNGG 2 cut(s) 108, 548
SetI ASST 9 cut(s) 9, 70, 201, 260, 508, 577, 610, 628, 646
SfaNI GCATC 3 cut(s) 134, 541, 589
SfuI TTCGAA 1 cut(s) 329
Sse9I AATT 3 cut(s) 11, 419, 500
SsiI CCGC 1 cut(s) 30
SspI AATATT 1 cut(s) 311
SspMI CTAG 4 cut(s) 65, 135, 159, 294
StyD4I CCNGG 2 cut(s) 106, 546
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 2 cut(s) 354, 463
TaqI TCGA 1 cut(s) 329
TasI AATT 3 cut(s) 11, 419, 500
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 2 cut(s) 173, 315
Tru9I TTAA 2 cut(s) 173, 315
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 6 cut(s) 68, 578, 581, 590, 608, 644
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 5 cut(s) 82, 294, 335, 513, 537
TspGWI ACGGA 2 cut(s) 633, 651
TspRI CASTG 1 cut(s) 190
XbaI TCTAGA 1 cut(s) 134
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
XmaJI CCTAGG 1 cut(s) 293
XspI CTAG 4 cut(s) 65, 135, 159, 294
Zsp2I ATGCAT 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.