pycom01g07060
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000468
Physical Location & Seq
Forward (+)
7650110 .. 7652529
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g07060.4

Sequence Viewer

Length: 615 bp
ATGCACGGCGCTCTCCAGGCGCTCTCTCTTCCTCTCATCCCAGACATGGATCAAAGAAAGATTGTGGCATGCTTCATTGTTAACGTGTGGTACTTGAATATTGTGACTTGGTTGATGATGTGGTTCATCAAGAATCATAATTCAGCTAGAAGGCGGAATCGGCAACTTGCACGTAGAAATCACACATTTGTTAGGAGAATGGTAGGGTCAACCTATTTAGATAGCATAATAGGATATGATGATGTTCAGTGTGTGAACCAAATTAGAATGGATAGAAAAACGTTCCATTATTTATGTCAAATGCTTCATGACCACGGTAGAGTAAAATTGGATGGTGTAGTGACAATGGAAGAACAAGTTTGCATGTTTTTGCATATACTTGCACATCATGTCAAGAATCGTACAATTGGGAGTAGGTTTTGTCGATCGGGGGAGACCATAAGTAGGTATTTCAATTCCATTCTACATGGAGTGTTAAGGCTACAAGGTACACTTTTGAAAGTGCTAGAACCCATTCCTAATATGATTGAGAGTGTTCAACCTACACAAGAATGGATTGCATGGAGAAACACTTTGGCTATGAGTATGTACAATGAGTGGCATGCACAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

205

Amino Acids

24.09

Weight (kDa)

9.67

Isoelectric Point (pI)

50.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 154
AclI AACGTT 1 cut(s) 281
AclWI GGATC 1 cut(s) 57
AfaI GTAC 4 cut(s) 92, 403, 490, 590
AfiI CCNNNNNNNGG 2 cut(s) 46, 444
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 4 cut(s) 97, 454, 499, 539
AjnI CCWGG 1 cut(s) 15
AleI CACNNNNGTG 1 cut(s) 610
AluBI AGCT 1 cut(s) 146
AluI AGCT 1 cut(s) 146
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 1 cut(s) 57
Asp700I GAANNNNTTC 1 cut(s) 513
AspLEI GCGC 2 cut(s) 11, 22
BccI CCATC 1 cut(s) 326
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 17
BcoDI GTCTC 1 cut(s) 428
BfaI CTAG 2 cut(s) 147, 506
BfoI RGCGCY 2 cut(s) 12, 23
Bme1390I CCNGG 1 cut(s) 17
BmrFI CCNGG 1 cut(s) 17
BsaAI YACGTR 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 428
BsaJI CCNNGG 1 cut(s) 313
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 444
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 1 cut(s) 313
BseGI GGATG 2 cut(s) 36, 337
BseLI CCNNNNNNNGG 2 cut(s) 46, 444
Bsh1285I CGRYCG 1 cut(s) 428
BsiEI CGRYCG 1 cut(s) 428
BslI CCNNNNNNNGG 2 cut(s) 46, 444
BsmAI GTCTC 1 cut(s) 428
Bso31I GGTCTC 1 cut(s) 428
Bsp1407I TGTACA 1 cut(s) 588
Bsp143I GATC 2 cut(s) 49, 425
BspACI CCGC 1 cut(s) 154
BspHI TCATGA 1 cut(s) 307
BspPI GGATC 1 cut(s) 57
BspTNI GGTCTC 1 cut(s) 428
BsrGI TGTACA 1 cut(s) 588
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 2 cut(s) 49, 425
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 1 cut(s) 317
Bst6I CTCTTC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 588
BstBAI YACGTR 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 70, 603
BstDSI CCRYGG 1 cut(s) 313
BstF5I GGATG 2 cut(s) 36, 337
BstH2I RGCGCY 2 cut(s) 12, 23
BstHHI GCGC 2 cut(s) 11, 22
BstKTI GATC 2 cut(s) 52, 428
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 2 cut(s) 49, 425
BstMCI CGRYCG 1 cut(s) 428
BstMWI GCNNNNNNNGC 2 cut(s) 17, 160
BstNI CCWGG 1 cut(s) 17
BstNSI RCATGY 3 cut(s) 72, 367, 605
BstSCI CCNGG 1 cut(s) 15
BtgI CCRYGG 1 cut(s) 313
BtsCI GGATG 2 cut(s) 36, 337
BtsIMutI CAGTG 1 cut(s) 254
Cac8I GCNNGC 2 cut(s) 70, 603
CciI TCATGA 1 cut(s) 307
CfoI GCGC 2 cut(s) 11, 22
Csp6I GTAC 4 cut(s) 91, 402, 489, 589
CviAII CATG 8 cut(s) 46, 69, 308, 364, 389, 467, 561, 602
CviJI RGCY 3 cut(s) 146, 481, 578
CviKI_1 RGCY 3 cut(s) 146, 481, 578
CviQI GTAC 4 cut(s) 91, 402, 489, 589
DpnI GATC 2 cut(s) 51, 427
DpnII GATC 2 cut(s) 49, 425
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
EciI GGCGGA 1 cut(s) 169
Eco31I GGTCTC 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 15
FaeI CATG 8 cut(s) 49, 72, 311, 367, 392, 470, 564, 605
FalI AAGNNNNNCTT 2 cut(s) 477, 509
FatI CATG 8 cut(s) 45, 68, 307, 363, 388, 466, 560, 601
FokI GGATG 2 cut(s) 23, 344
FspBI CTAG 2 cut(s) 147, 506
GlaI GCGC 2 cut(s) 10, 21
HaeII RGCGCY 2 cut(s) 12, 23
HhaI GCGC 2 cut(s) 11, 22
Hin1II CATG 8 cut(s) 49, 72, 311, 367, 392, 470, 564, 605
Hin6I GCGC 2 cut(s) 9, 20
HinP1I GCGC 2 cut(s) 9, 20
HincII GTYRAC 2 cut(s) 82, 210
HindII GTYRAC 2 cut(s) 82, 210
HinfI GANTC 3 cut(s) 133, 157, 397
HpaI GTTAAC 1 cut(s) 82
Hpy166II GTNNAC 4 cut(s) 82, 210, 256, 491
Hpy188III TCNNGA 3 cut(s) 130, 308, 394
Hpy8I GTNNAC 4 cut(s) 82, 210, 256, 491
HpyAV CCTTC 1 cut(s) 144
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 3 cut(s) 84, 172, 281
HpyCH4V TGCA 7 cut(s) 4, 170, 363, 373, 383, 560, 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 160
HpySE526I ACGT 3 cut(s) 84, 172, 281
Hsp92II CATG 8 cut(s) 49, 72, 311, 367, 392, 470, 564, 605
HspAI GCGC 2 cut(s) 9, 20
KspAI GTTAAC 1 cut(s) 82
Kzo9I GATC 2 cut(s) 49, 425
LpnPI CCDG 3 cut(s) 2, 29, 54
MaeI CTAG 2 cut(s) 147, 506
MaeII ACGT 3 cut(s) 84, 172, 281
MaeIII GTNAC 2 cut(s) 103, 340
MalI GATC 2 cut(s) 51, 427
MboI GATC 2 cut(s) 49, 425
MboII GAAGA 2 cut(s) 20, 362
MfeI CAATTG 1 cut(s) 405
MluCI AATT 5 cut(s) 139, 261, 326, 405, 454
MnlI CCTC 1 cut(s) 42
MroXI GAANNNNTTC 1 cut(s) 513
MseI TTAA 2 cut(s) 81, 476
MslI CAYNNNNRTG 2 cut(s) 550, 610
MspR9I CCNGG 1 cut(s) 17
MunI CAATTG 1 cut(s) 405
MvaI CCWGG 1 cut(s) 17
MwoI GCNNNNNNNGC 2 cut(s) 17, 160
NdeII GATC 2 cut(s) 49, 425
NlaIII CATG 8 cut(s) 49, 72, 311, 367, 392, 470, 564, 605
NmuCI GTSAC 2 cut(s) 103, 340
NspI RCATGY 3 cut(s) 72, 367, 605
OliI CACNNNNGTG 1 cut(s) 610
PaeI GCATGC 2 cut(s) 72, 605
PagI TCATGA 1 cut(s) 307
PdmI GAANNNNTTC 1 cut(s) 513
PfeI GAWTC 3 cut(s) 133, 157, 397
Ple19I CGATCG 1 cut(s) 428
Ppu21I YACGTR 1 cut(s) 173
Psp1406I AACGTT 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 15
PspGI CCWGG 1 cut(s) 15
PvuI CGATCG 1 cut(s) 428
RsaI GTAC 4 cut(s) 92, 403, 490, 590
RsaNI GTAC 4 cut(s) 91, 402, 489, 589
RseI CAYNNNNRTG 2 cut(s) 550, 610
SaqAI TTAA 2 cut(s) 81, 476
Sau3AI GATC 2 cut(s) 49, 425
ScrFI CCNGG 1 cut(s) 17
SetI ASST 9 cut(s) 87, 148, 175, 215, 284, 419, 449, 490, 544
SmiMI CAYNNNNRTG 2 cut(s) 550, 610
SphI GCATGC 2 cut(s) 72, 605
Sse9I AATT 5 cut(s) 139, 261, 326, 405, 454
SsiI CCGC 1 cut(s) 154
SspI AATATT 1 cut(s) 100
SspMI CTAG 2 cut(s) 147, 506
StyD4I CCNGG 1 cut(s) 15
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 3 cut(s) 87, 175, 284
TaqI TCGA 1 cut(s) 424
TasI AATT 5 cut(s) 139, 261, 326, 405, 454
TatI WGTACW 1 cut(s) 588
TfiI GAWTC 3 cut(s) 133, 157, 397
Tru1I TTAA 2 cut(s) 81, 476
Tru9I TTAA 2 cut(s) 81, 476
TscAI CASTG 1 cut(s) 254
TseFI GTSAC 2 cut(s) 103, 340
Tsp45I GTSAC 2 cut(s) 103, 340
TspDTI ATGAA 3 cut(s) 64, 115, 296
TspRI CASTG 1 cut(s) 254
XceI RCATGY 3 cut(s) 72, 367, 605
XmnI GAANNNNTTC 1 cut(s) 513
XspI CTAG 2 cut(s) 147, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.