Rorug04G0076800
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000468
Physical Location & Seq
Forward (+)
11844975 .. 11856183
11209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0076800.1

Sequence Viewer

Length: 2964 bp
ATGGCTGAGGCTGTTGTTTCTTTTCTAGTGGAAAGAGTTGGTGACTTCATCATTCAAGAAGGCAGATCCTTGTCCGGAGTTCGCGATCAAGTTGAGCGTGCACATACAGACCTACTATTCATCAAGGGCTTCCTCAAAGATGCAGACGCAAAACGACGAGATGGTGAGGCAATACGCATTTTTGTTGCCAAAATTAGAGATGCTGCTTATGATTTGGAGGATGTCATTGAAGCTTTTGTCTTGAAGGTGGATTCCAAGAGGAAAGGAGGTATACAGAATGTTTTGAAGAGGTTGGGGTGCATCTTCATTGAAGGAGTTCGTCGTCACAAGATTGGGTCAGAAATTGGGGTAATTACTATCACGATTTCTGATTTGAAGCAGAACTTGCAAACTTATGGTATCAAGGAGATAAGGGATTCCTCTACTTCCCTCCAATTGTGTGAAGGACAACAACTACTGAGGCGAACTTACACTCATGTTATTGATCGTAATGTGGTCGGGGTAGAGGATTCTGTCCATGAATTGGTTGGGCATTTGGTGAAGGAAGAGCATCGCCACCGAGTTGTTTCTATTATTGGGATGGGCGGATCTGGGAAAACAACTATTGCTAAACAAGTTTATCATCACAAAGAGGTAACAGACCATTTCGATTGTTTGGCTTGGGTTTGTATATCTCAACAATATCAAGTCAGGGATGTCTTGGAGAGGATTTATGTCAAACTTCTTTGCCCCACAAAAGAGGAAAGAGAAGAAATCAAAAATTTGAATGATGACGAAATACCAGGGAGGCTTTCTCGTCTCCAAAATGAAAAAAAATGTTTGATTGTCCTTGATGACATTTGGAAAAGAGAGACCTGGGACTTTTTGAAGGCTGCATTTGAATGCCATGAAGACTCAAAGAGCAGAATACTACTTACTACACGCAATGAACAAGTAGCTTCGCATGCCGATGAGAACGGTTTTCACTACCGACCTCCAGCACTAAATGAGTCTGAGAGTTGGGAGCTGTTTGAGAAGATTGCAATGAGTGGAAGGAAAGCTGGAATAGATGCTGAAGTTTACACGAAGATGGAAGAATTAGGAAAAGAAATGGTTCAACATTGTAGGGGTCTGCCACTAGCTGTCATTGTACTTGCTGGACTTCTTGCTAGGAAAAACACAATTAAAGATTGGGTAACCATAAGAGAAAATGTTGGTGTTTACATAAGTAGAGGCTTTGGCCGCCTGGAAGAAGAACATGCATATGTATCATGGGTGTTGGCGTTGAGTTATGATGACTTGCCGTATCACTTAAAACCCTGTTTCCTATATTTAGGACATTTTCCCGAGGATCATGAGATCCGGATGAAGAGGTTGACAAGACTATGGATTGCAGAAGGTTTTATTTCTTTGACAAAACAAAGGCAAAGTTTAGGGGAAACAATGGAGGATGTGGCATACAATTGGTTGAATGAGTTGGTAGAAAGGTGTGTGATTCAAGTAGGAGAGAGAAGTTCAACTTTTGAGAATATTAAAACTTGCCGGATACATGATCTTTTGCGAGATTTGTGTTTGAGGAAGGCGGAAGAGGAAAATTTTCTCCAAATTGTCAAAGGTTCTCATAAAAATGAGGCAATGCATCCAGTTTCTTCTTCTTCTTCCATGGTAACCTATGTATCATCAATGGGTAAGATTCGAAGACTTGCCGTCTACTTGGATGAGAATGATGATAAATTGGTATCCTCACTTGGGGAAAGAGGTGGACACCTTAGGTCCTTGTTATATTTTGGCCCCAGCGATAATTATTGGTTTCCTAAAAGGAAAGAATTAATACCTTCCATGTTCAAGGATTTCAAATTACTCAGATCATTGATGATTGAAGGTATGAATGATGGAGAAGTAAACATAGAGCTGCCTAGAGAAATTGGAAGCATGGTCCACTTGAGGTTTCTGAGTCTAAGGAACAGTAATATAAAATCGTTTCCTCCGTTCCTAGGAAACTTGATTTGTTTGCAGACACTTGATTTCCGTGTTACATATAACTTCATGTTTATCCCAAATGTGATATGCAAGATGAATAAGTTGAGACATTTATACATTCCATTGTGGTACAGGGCTAAAGGTACACTTGATTTCCGTGTTACATATAACTGCATGTTTATCCCAAATGTGATATGCAAGATGAATAAGTTGAGACATTTATACCTTCCATTGTGGTACAGGGCTAAAGGTAAACTGCAGCTATCTACTCTCCGTGATTTACAAACTTTAAATTCTGTTTCGAGTAGATATTGTGATTTGAATGATCTTGCTAAACTAGGCAATCTAAGGAAACTGAAAATAAGATTGTCATCTAGCCAATCGAAAAATCTGGAGAAAATCTTGGATGCGGCAGGCTGCGTGCTCAACGGTATTCGGTCCCTGTGGTTGGTCAACGATGTTGGAATGGAAAGCGGTGCAGAGGTTAGTCAAATAGTTGCAAGGTGCGGTCAAATATACAAGTTGGAGCTGTGGGGGCCCACACTAGAACTACCGAAAGAGTTCCACTCCTATTCAAACCTCACCAAGTTACGTTTGTATGGATGTGCTCTCAAGGGAGACCAAATGGCCGTACTAGAGAAGTTGCCCAACCTGAAAAGTCTTGATCTGCGGGGTAATTTGTTTGAGGAAAATACAAAAGTATTGGTTTTCTCCGCTGGAAGCTTTCCTCGTCTTCAGTTTCTTACCTGTGGGCTTCTCAAAGGAATAACAGAGTGGAGGGTGGAGGAAGGAGCCATGCCTTGTCTCTGCAAATTGGTCATTGATTGTTGCAGCCGATGCCGATTGAGTACGGTTCCAGATGGGTTGAGACACCTTACTACCCTCAAGGAATTGAAGATTGAGCATATGCCTAAACTATTTTGTAGTAAGCTTGAGGAAGGAGGAGAAGACTTCCATAAAGTCCAACACATCCCTTCCCTTGTAATCCAAAATACGTATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

987

Amino Acids

113.37

Weight (kDa)

8.53

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 88 1.5e-20 Rx N-terminal domain
NB-ARC PF00931 169 - 344 1.8e-43 NB-ARC domain
WHD_DRP PF23559 441 - 517 1.2e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 597 - 694 2.1e-14 Leucine-rich repeat region
LRR_14 PF23598 702 - 954 4.1e-23 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 523
AccI GTMKAC 2 cut(s) 271, 1689
AccII CGCG 1 cut(s) 84
AccIII TCCGGA 2 cut(s) 74, 1341
AciI CCGC 8 cut(s) 585, 1222, 1562, 2369, 2433, 2466, 2629, 2673
AclWI GGATC 4 cut(s) 60, 595, 1333, 1338
AcoI YGGCCR 2 cut(s) 1219, 2586
AcsI RAATTY 3 cut(s) 760, 1573, 2251
AcuI CTGAAG 2 cut(s) 1074, 2678
AfaI GTAC 6 cut(s) 1131, 2090, 2104, 2198, 2592, 2809
AfiI CCNNNNNNNGG 4 cut(s) 523, 1728, 1973, 2407
AhdI GACNNNNNGTC 1 cut(s) 1685
AjnI CCWGG 3 cut(s) 781, 854, 1224
Alw21I GWGCWC 3 cut(s) 103, 2385, 2569
Alw26I GTCTC 7 cut(s) 803, 845, 2059, 2167, 2571, 2768, 2821
Alw44I GTGCAC 1 cut(s) 99
AlwI GGATC 4 cut(s) 60, 595, 1333, 1338
Ama87I CYCGRG 1 cut(s) 1325
Aor13HI TCCGGA 2 cut(s) 74, 1341
AoxI GGCC 4 cut(s) 1219, 1768, 2495, 2586
ApaI GGGCCC 1 cut(s) 2499
ApaLI GTGCAC 1 cut(s) 99
ApeKI GCWGC 6 cut(s) 203, 872, 1891, 2218, 2376, 2790
ApoI RAATTY 3 cut(s) 760, 1573, 2251
AseI ATTAAT 1 cut(s) 1808
Asp700I GAANNNNTTC 4 cut(s) 315, 1092, 1575, 2519
AspA2I CCTAGG 1 cut(s) 1972
AspS9I GGNCC 6 cut(s) 1752, 1769, 1915, 2397, 2495, 2496
AsuHPI GGTGA 4 cut(s) 53, 176, 550, 2533
AsuII TTCGAA 1 cut(s) 1675
AvaI CYCGRG 1 cut(s) 1325
AvaII GGWCC 3 cut(s) 1752, 1915, 2397
AvrII CCTAGG 1 cut(s) 1972
AxyI CCTNAGG 1 cut(s) 1748
BaeGI GKGCMC 2 cut(s) 103, 2499
BaeI ACNNNNGTAYC 2 cut(s) 1638, 1671
BanII GRGCYC 1 cut(s) 2499
BbsI GAAGAC 4 cut(s) 897, 1684, 2684, 2913
Bbv12I GWGCWC 3 cut(s) 103, 2385, 2569
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 6 cut(s) 190, 859, 1878, 2230, 2363, 2802
BccI CCATC 5 cut(s) 155, 574, 1063, 1865, 2813
BceAI ACGGC 3 cut(s) 1267, 1670, 2573
BciT130I CCWGG 3 cut(s) 783, 856, 1226
BciVI GTATCC 2 cut(s) 1518, 1729
BcoDI GTCTC 7 cut(s) 803, 845, 2059, 2167, 2571, 2768, 2821
BfaI CTAG 9 cut(s) 26, 1118, 1149, 1896, 1973, 2297, 2334, 2504, 2594
BfmI CTRYAG 1 cut(s) 2216
BfuI GTATCC 2 cut(s) 1518, 1729
BisI GCNGC 8 cut(s) 204, 873, 1222, 1892, 2219, 2370, 2377, 2791
BlnI CCTAGG 1 cut(s) 1972
BlsI GCNGC 8 cut(s) 205, 874, 1223, 1893, 2220, 2371, 2378, 2792
Bme1390I CCNGG 3 cut(s) 783, 856, 1226
Bme18I GGWCC 3 cut(s) 1752, 1915, 2397
BmeRI GACNNNNNGTC 1 cut(s) 1685
BmeT110I CYCGRG 1 cut(s) 1325
BmgT120I GGNCC 6 cut(s) 1752, 1769, 1915, 2397, 2495, 2496
BmiI GGNNCC 6 cut(s) 1771, 2399, 2496, 2497, 2752, 2814
BmrFI CCNGG 3 cut(s) 783, 856, 1226
BmsI GCATC 8 cut(s) 130, 190, 309, 559, 1039, 1627, 2356, 2786
BpiI GAAGAC 4 cut(s) 897, 1684, 2684, 2913
BplI GAGNNNNNCTC 4 cut(s) 778, 810, 2510, 2542
BpmI CTGGAG 2 cut(s) 960, 2372
Bpu10I CCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 1675
BpuEI CTTGAG 4 cut(s) 1942, 2555, 2828, 2912
BsaAI YACGTR 1 cut(s) 2955
BsaBI GATNNNNATC 1 cut(s) 2329
BsaI GGTCTC 2 cut(s) 845, 2571
BsaJI CCNNGG 5 cut(s) 782, 855, 1326, 1641, 1972
BsaWI WCCGGW 2 cut(s) 74, 1341
Bsc4I CCNNNNNNNGG 4 cut(s) 523, 1728, 1973, 2407
Bse1I ACTGG 1 cut(s) 1622
Bse21I CCTNAGG 1 cut(s) 1748
Bse3DI GCAATG 3 cut(s) 931, 1029, 1620
Bse8I GATNNNNATC 1 cut(s) 2329
BseAI TCCGGA 2 cut(s) 74, 1341
BseBI CCWGG 3 cut(s) 783, 856, 1226
BseDI CCNNGG 5 cut(s) 782, 855, 1326, 1641, 1972
BseJI GATNNNNATC 1 cut(s) 2329
BseLI CCNNNNNNNGG 4 cut(s) 523, 1728, 1973, 2407
BseMI GCAATG 3 cut(s) 931, 1029, 1620
BseMII CTCAG 4 cut(s) 449, 984, 1855, 1922
BseNI ACTGG 1 cut(s) 1622
BseRI GAGGAG 1 cut(s) 2916
BseSI GKGCMC 2 cut(s) 103, 2499
BseXI GCAGC 6 cut(s) 190, 859, 1878, 2230, 2363, 2802
BseYI CCCAGC 1 cut(s) 1772
BsgI GTGCAG 1 cut(s) 2457
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 4 cut(s) 1221, 1770, 2497, 2588
BsiHKAI GWGCWC 3 cut(s) 103, 2385, 2569
BsiHKCI CYCGRG 1 cut(s) 1325
BsiSI CCGG 3 cut(s) 75, 1342, 1522
BslFI GGGAC 2 cut(s) 872, 2383
BslI CCNNNNNNNGG 4 cut(s) 523, 1728, 1973, 2407
BsmAI GTCTC 7 cut(s) 803, 845, 2059, 2167, 2571, 2768, 2821
BsmBI CGTCTC 1 cut(s) 803
BsmFI GGGAC 2 cut(s) 872, 2383
BsmI GAATGC 1 cut(s) 887
BsnI GGCC 4 cut(s) 1221, 1770, 2497, 2588
Bso31I GGTCTC 2 cut(s) 845, 2571
BsoBI CYCGRG 1 cut(s) 1325
Bsp119I TTCGAA 1 cut(s) 1675
Bsp120I GGGCCC 1 cut(s) 2495
Bsp1286I GDGCHC 4 cut(s) 103, 2385, 2499, 2569
Bsp13I TCCGGA 2 cut(s) 74, 1341
Bsp19I CCATGG 1 cut(s) 1641
Bsp68I TCGCGA 1 cut(s) 84
BspACI CCGC 8 cut(s) 585, 1222, 1562, 2369, 2433, 2466, 2629, 2673
BspANI GGCC 4 cut(s) 1221, 1770, 2497, 2588
BspCNI CTCAG 4 cut(s) 450, 985, 1854, 1923
BspEI TCCGGA 2 cut(s) 74, 1341
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 1 cut(s) 1333
BspLI GGNNCC 6 cut(s) 1771, 2399, 2496, 2497, 2752, 2814
BspMAI CTGCAG 1 cut(s) 2220
BspPI GGATC 4 cut(s) 60, 595, 1333, 1338
BspQI GCTCTTC 1 cut(s) 540
BspT104I TTCGAA 1 cut(s) 1675
BspTNI GGTCTC 2 cut(s) 845, 2571
BsrDI GCAATG 3 cut(s) 931, 1029, 1620
BsrI ACTGG 1 cut(s) 1622
BssECI CCNNGG 5 cut(s) 782, 855, 1326, 1641, 1972
BssNAI GTATAC 1 cut(s) 272
BssT1I CCWWGG 2 cut(s) 1641, 1972
Bst1107I GTATAC 1 cut(s) 272
Bst2UI CCWGG 3 cut(s) 783, 856, 1226
Bst4CI ACNGT 4 cut(s) 959, 1946, 2390, 2812
Bst6I CTCTTC 4 cut(s) 281, 540, 1343, 1560
BstAPI GCANNNNNTGC 2 cut(s) 385, 2796
BstBAI YACGTR 1 cut(s) 2955
BstBI TTCGAA 1 cut(s) 1675
BstC8I GCNNGC 4 cut(s) 99, 945, 2374, 2381
BstDEI CTNAG 8 cut(s) 6, 458, 993, 1748, 1841, 1931, 1937, 2306
BstDSI CCRYGG 1 cut(s) 1641
BstEII GGTNACC 2 cut(s) 1174, 1645
BstFNI CGCG 1 cut(s) 84
BstMAI GTCTC 7 cut(s) 803, 845, 2059, 2167, 2571, 2768, 2821
BstMWI GCNNNNNNNGC 5 cut(s) 385, 944, 1221, 2494, 2796
BstNI CCWGG 3 cut(s) 783, 856, 1226
BstNSI RCATGY 3 cut(s) 947, 1241, 2137
BstPI GGTNACC 2 cut(s) 1174, 1645
BstSCI CCNGG 3 cut(s) 781, 854, 1224
BstSFI CTRYAG 1 cut(s) 2216
BstSLI GKGCMC 2 cut(s) 103, 2499
BstSNI TACGTA 1 cut(s) 2955
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 6 cut(s) 190, 859, 1878, 2230, 2363, 2802
BstV2I GAAGAC 4 cut(s) 897, 1684, 2684, 2913
BstX2I RGATCY 3 cut(s) 65, 587, 1338
BstYI RGATCY 3 cut(s) 65, 587, 1338
BstZ17I GTATAC 1 cut(s) 272
Bsu36I CCTNAGG 1 cut(s) 1748
BsuI GTATCC 2 cut(s) 1518, 1729
BsuRI GGCC 4 cut(s) 1221, 1770, 2497, 2588
BtgI CCRYGG 1 cut(s) 1641
BtgZI GCGATG 1 cut(s) 536
BtuMI TCGCGA 1 cut(s) 84
Cac8I GCNNGC 4 cut(s) 99, 945, 2374, 2381
CciI TCATGA 1 cut(s) 1333
Cfr13I GGNCC 6 cut(s) 1752, 1769, 1915, 2397, 2495, 2496
CseI GACGC 1 cut(s) 155
Csp6I GTAC 6 cut(s) 1130, 2089, 2103, 2197, 2591, 2808
CviQI GTAC 6 cut(s) 1130, 2089, 2103, 2197, 2591, 2808
DdeI CTNAG 8 cut(s) 6, 458, 993, 1748, 1841, 1931, 1937, 2306
DraI TTTAAA 1 cut(s) 2250
DriI GACNNNNNGTC 1 cut(s) 1685
EaeI YGGCCR 2 cut(s) 1219, 2586
Eam1104I CTCTTC 4 cut(s) 281, 540, 1343, 1560
Eam1105I GACNNNNNGTC 1 cut(s) 1685
EarI CTCTTC 4 cut(s) 281, 540, 1343, 1560
EciI GGCGGA 2 cut(s) 600, 1577
Eco105I TACGTA 1 cut(s) 2955
Eco130I CCWWGG 2 cut(s) 1641, 1972
Eco24I GRGCYC 1 cut(s) 2499
Eco31I GGTCTC 2 cut(s) 845, 2571
Eco47I GGWCC 3 cut(s) 1752, 1915, 2397
Eco57I CTGAAG 2 cut(s) 1074, 2678
Eco81I CCTNAGG 1 cut(s) 1748
Eco88I CYCGRG 1 cut(s) 1325
Eco91I GGTNACC 2 cut(s) 1174, 1645
EcoO109I RGGNCCY 2 cut(s) 1752, 2495
EcoO65I GGTNACC 2 cut(s) 1174, 1645
EcoRII CCWGG 3 cut(s) 781, 854, 1224
EcoT14I CCWWGG 2 cut(s) 1641, 1972
EcoT22I ATGCAT 2 cut(s) 1243, 1620
EcoT38I GRGCYC 1 cut(s) 2499
ErhI CCWWGG 2 cut(s) 1641, 1972
Esp3I CGTCTC 1 cut(s) 803
FalI AAGNNNNNCTT 6 cut(s) 368, 400, 1483, 1515, 2091, 2123
FaqI GGGAC 2 cut(s) 872, 2383
FauI CCCGC 1 cut(s) 2622
FauNDI CATATG 2 cut(s) 1243, 2865
FblI GTMKAC 2 cut(s) 271, 1689
Fnu4HI GCNGC 8 cut(s) 204, 873, 1222, 1892, 2219, 2370, 2377, 2791
FriOI GRGCYC 1 cut(s) 2499
Fsp4HI GCNGC 8 cut(s) 204, 873, 1222, 1892, 2219, 2370, 2377, 2791
FspBI CTAG 9 cut(s) 26, 1118, 1149, 1896, 1973, 2297, 2334, 2504, 2594
GluI GCNGC 8 cut(s) 204, 873, 1222, 1892, 2219, 2370, 2377, 2791
GsaI CCCAGC 1 cut(s) 1776
GsuI CTGGAG 2 cut(s) 960, 2372
HaeIII GGCC 4 cut(s) 1221, 1770, 2497, 2588
HapII CCGG 3 cut(s) 75, 1342, 1522
HgaI GACGC 1 cut(s) 155
HincII GTYRAC 2 cut(s) 1356, 2413
HindII GTYRAC 2 cut(s) 1356, 2413
HindIII AAGCTT 3 cut(s) 231, 2680, 2888
HinfI GANTC 8 cut(s) 251, 416, 509, 893, 989, 1474, 1672, 1933
HpaII CCGG 3 cut(s) 75, 1342, 1522
HphI GGTGA 4 cut(s) 53, 176, 550, 2533
Hpy188I TCNGA 5 cut(s) 340, 370, 994, 1844, 1932
Hpy99I CGWCG 2 cut(s) 159, 324
HpyCH4III ACNGT 4 cut(s) 959, 1946, 2390, 2812
HpyCH4IV ACGT 2 cut(s) 2551, 2954
HpyF10VI GCNNNNNNNGC 5 cut(s) 385, 944, 1221, 2494, 2796
HpyF3I CTNAG 8 cut(s) 6, 458, 993, 1748, 1841, 1931, 1937, 2306
HpySE526I ACGT 2 cut(s) 2551, 2954
Kpn2I TCCGGA 2 cut(s) 74, 1341
LguI GCTCTTC 1 cut(s) 540
LmnI GCTCC 3 cut(s) 1003, 2485, 2750
Lsp1109I GCAGC 6 cut(s) 190, 859, 1878, 2230, 2363, 2802
LweI GCATC 8 cut(s) 130, 190, 309, 559, 1039, 1627, 2356, 2786
MaeI CTAG 9 cut(s) 26, 1118, 1149, 1896, 1973, 2297, 2334, 2504, 2594
MaeII ACGT 2 cut(s) 2551, 2954
MaeIII GTNAC 8 cut(s) 41, 323, 634, 1174, 1645, 2011, 2119, 2547
MfeI CAATTG 2 cut(s) 434, 1441
MflI RGATCY 3 cut(s) 65, 587, 1338
MhlI GDGCHC 4 cut(s) 103, 2385, 2499, 2569
MlyI GAGTC 3 cut(s) 887, 998, 1942
MmeI TCCRAC 3 cut(s) 2401, 2463, 2947
Mph1103I ATGCAT 2 cut(s) 1243, 1620
MroI TCCGGA 2 cut(s) 74, 1341
MroXI GAANNNNTTC 4 cut(s) 315, 1092, 1575, 2519
MseI TTAA 6 cut(s) 1164, 1292, 1512, 1808, 2249, 2962
MslI CAYNNNNRTG 5 cut(s) 880, 948, 1067, 1242, 1605
MspA1I CMGCKG 1 cut(s) 2675
MspI CCGG 3 cut(s) 75, 1342, 1522
MspR9I CCNGG 3 cut(s) 783, 856, 1226
MunI CAATTG 2 cut(s) 434, 1441
Mva1269I GAATGC 1 cut(s) 887
MvaI CCWGG 3 cut(s) 783, 856, 1226
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 5 cut(s) 385, 944, 1221, 2494, 2796
NcoI CCATGG 1 cut(s) 1641
NdeI CATATG 2 cut(s) 1243, 2865
NlaIV GGNNCC 6 cut(s) 1771, 2399, 2496, 2497, 2752, 2814
NmuCI GTSAC 2 cut(s) 41, 323
NruI TCGCGA 1 cut(s) 84
NsiI ATGCAT 2 cut(s) 1243, 1620
NspI RCATGY 3 cut(s) 947, 1241, 2137
NspV TTCGAA 1 cut(s) 1675
PaeI GCATGC 1 cut(s) 947
PagI TCATGA 1 cut(s) 1333
PciSI GCTCTTC 1 cut(s) 540
PcsI WCGNNNNNNNCGW 1 cut(s) 1964
PctI GAATGC 1 cut(s) 887
PdmI GAANNNNTTC 4 cut(s) 315, 1092, 1575, 2519
PfeI GAWTC 5 cut(s) 251, 416, 509, 1474, 1672
PflMI CCANNNNNTGG 1 cut(s) 523
PkrI GCNGC 8 cut(s) 205, 874, 1223, 1893, 2220, 2371, 2378, 2792
PleI GAGTC 3 cut(s) 887, 997, 1941
PpsI GAGTC 3 cut(s) 887, 997, 1941
Ppu21I YACGTR 1 cut(s) 2955
PpuMI RGGWCCY 1 cut(s) 1752
PshBI ATTAAT 1 cut(s) 1808
Psp5II RGGWCCY 1 cut(s) 1752
Psp6I CCWGG 3 cut(s) 781, 854, 1224
PspEI GGTNACC 2 cut(s) 1174, 1645
PspFI CCCAGC 1 cut(s) 1772
PspGI CCWGG 3 cut(s) 781, 854, 1224
PspN4I GGNNCC 6 cut(s) 1771, 2399, 2496, 2497, 2752, 2814
PspOMI GGGCCC 1 cut(s) 2495
PspPI GGNCC 6 cut(s) 1752, 1769, 1915, 2397, 2495, 2496
PspPPI RGGWCCY 1 cut(s) 1752
PstI CTGCAG 1 cut(s) 2220
PsuI RGATCY 3 cut(s) 65, 587, 1338
RruI TCGCGA 1 cut(s) 84
RsaI GTAC 6 cut(s) 1131, 2090, 2104, 2198, 2592, 2809
RsaNI GTAC 6 cut(s) 1130, 2089, 2103, 2197, 2591, 2808
RseI CAYNNNNRTG 5 cut(s) 880, 948, 1067, 1242, 1605
SapI GCTCTTC 1 cut(s) 540
SaqAI TTAA 6 cut(s) 1164, 1292, 1512, 1808, 2249, 2962
SatI GCNGC 8 cut(s) 204, 873, 1222, 1892, 2219, 2370, 2377, 2791
Sau96I GGNCC 6 cut(s) 1752, 1769, 1915, 2397, 2495, 2496
SchI GAGTC 3 cut(s) 887, 998, 1942
ScrFI CCNGG 3 cut(s) 783, 856, 1226
SduI GDGCHC 4 cut(s) 103, 2385, 2499, 2569
SfaNI GCATC 8 cut(s) 130, 190, 309, 559, 1039, 1627, 2356, 2786
SfcI CTRYAG 1 cut(s) 2216
SfuI TTCGAA 1 cut(s) 1675
SinI GGWCC 3 cut(s) 1752, 1915, 2397
SmiMI CAYNNNNRTG 5 cut(s) 880, 948, 1067, 1242, 1605
SmlI CTYRAG 4 cut(s) 1921, 2570, 2843, 2891
SmoI CTYRAG 4 cut(s) 1921, 2570, 2843, 2891
SnaBI TACGTA 1 cut(s) 2955
SphI GCATGC 1 cut(s) 947
SsiI CCGC 8 cut(s) 585, 1222, 1562, 2369, 2433, 2466, 2629, 2673
SspI AATATT 1 cut(s) 1510
SspMI CTAG 9 cut(s) 26, 1118, 1149, 1896, 1973, 2297, 2334, 2504, 2594
StyD4I CCNGG 3 cut(s) 781, 854, 1224
StyI CCWWGG 2 cut(s) 1641, 1972
TaaI ACNGT 4 cut(s) 959, 1946, 2390, 2812
TaiI ACGT 2 cut(s) 2554, 2957
TaqI TCGA 4 cut(s) 648, 1675, 2261, 2342
TaqII GACCGA 1 cut(s) 2385
TatI WGTACW 1 cut(s) 1129
TauI GCSGC 2 cut(s) 1224, 2372
TfiI GAWTC 5 cut(s) 251, 416, 509, 1474, 1672
Tru1I TTAA 6 cut(s) 1164, 1292, 1512, 1808, 2249, 2962
Tru9I TTAA 6 cut(s) 1164, 1292, 1512, 1808, 2249, 2962
TseFI GTSAC 2 cut(s) 41, 323
TseI GCWGC 6 cut(s) 203, 872, 1891, 2218, 2376, 2790
Tsp45I GTSAC 2 cut(s) 41, 323
TspGWI ACGGA 4 cut(s) 1956, 1997, 2105, 2222
Van91I CCANNNNNTGG 1 cut(s) 523
VneI GTGCAC 1 cut(s) 99
VpaK11BI GGWCC 3 cut(s) 1752, 1915, 2397
VspI ATTAAT 1 cut(s) 1808
XapI RAATTY 3 cut(s) 760, 1573, 2251
XceI RCATGY 3 cut(s) 947, 1241, 2137
XcmI CCANNNNNNNNNTGG 1 cut(s) 524
XmaJI CCTAGG 1 cut(s) 1972
XmiI GTMKAC 2 cut(s) 271, 1689
XmnI GAANNNNTTC 4 cut(s) 315, 1092, 1575, 2519
XspI CTAG 9 cut(s) 26, 1118, 1149, 1896, 1973, 2297, 2334, 2504, 2594
Zsp2I ATGCAT 2 cut(s) 1243, 1620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.