pycom09g02020
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000468
Physical Location & Seq
Forward (+)
1485104 .. 1485864
761 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g02020.3

Sequence Viewer

Length: 600 bp
ATGGGACTTACATTAACGTTCATGTCCCTGAGCGAGATAAACCAAGATACCGATCAAGGAAGGGAGAAACAAGATTGTTTGACAACAGATCGATCGATATGTTGGGAGTTTGTAATAAGTAGACCATCGGGATTAAAAGTTCCCATGGGGTGTTACTACCTTGTGGACGGTGGGTACACAAATGGTCCAGGATACCTTGCATCGTATCGGGGAGTGCGTTACCATCTCTCGGAGTGGAGAAATAGAAGACTCCTGATTAATCATGAGGAGTATTTCAATATGAAACATGTTCAAGCTAGAAATGTCATTGAGAGATGTTTTGGACTACTTAATATGCGTTGGAGCATCTTAAGAGGTCCATCATTTTTCCTAATTAAAACACAATTTAGGATTATGACAGTCTGCTGCCTATTACATAATCTTATTAGAAGGCATATGTCAACTGATCCAATAGAAAATGAAATCCTAAACTTGGACGAATTTGAAAGTAGTGATGATGATGAAGATATGATTGAGAATGTTCAACCTACACAAGAATGGACTGCATGGAGAAACACTTTGGCTATGAATATGTACAATGAGTGGTGTGCACAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

200

Amino Acids

23.51

Weight (kDa)

5.39

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 121
AclI AACGTT 1 cut(s) 17
AclWI GGATC 1 cut(s) 440
AcsI RAATTY 1 cut(s) 479
AfaI GTAC 2 cut(s) 176, 575
AfiI CCNNNNNNNGG 2 cut(s) 229, 472
AflII CTTAAG 1 cut(s) 349
AflIII ACRYGT 1 cut(s) 286
AgsI TTSAA 4 cut(s) 277, 293, 485, 524
AjnI CCWGG 1 cut(s) 187
AleI CACNNNNGTG 1 cut(s) 595
AloI GAACNNNNNNTCC 2 cut(s) 123, 155
AluBI AGCT 1 cut(s) 296
AluI AGCT 1 cut(s) 296
Alw21I GWGCWC 1 cut(s) 592
Alw44I GTGCAC 1 cut(s) 588
AlwI GGATC 1 cut(s) 440
ApaLI GTGCAC 1 cut(s) 588
ApeKI GCWGC 1 cut(s) 405
ApoI RAATTY 1 cut(s) 479
AseI ATTAAT 1 cut(s) 258
AspS9I GGNCC 2 cut(s) 185, 356
AvaII GGWCC 2 cut(s) 185, 356
BaeGI GKGCMC 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 253
Bbv12I GWGCWC 1 cut(s) 592
BbvI GCAGC 1 cut(s) 392
BccI CCATC 3 cut(s) 133, 231, 367
BciT130I CCWGG 1 cut(s) 189
BciVI GTATCC 1 cut(s) 185
BfaI CTAG 1 cut(s) 297
BfrI CTTAAG 1 cut(s) 349
BfuI GTATCC 1 cut(s) 185
BisI GCNGC 1 cut(s) 406
BlsI GCNGC 1 cut(s) 407
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 2 cut(s) 185, 356
BmgT120I GGNCC 2 cut(s) 185, 356
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 2 cut(s) 209, 354
BpiI GAAGAC 1 cut(s) 253
Bpu10I CCTNAGC 1 cut(s) 29
Bsa29I ATCGAT 2 cut(s) 91, 95
BsaBI GATNNNNATC 1 cut(s) 51
BsaJI CCNNGG 1 cut(s) 144
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 472
Bse8I GATNNNNATC 1 cut(s) 51
BseBI CCWGG 1 cut(s) 189
BseCI ATCGAT 2 cut(s) 91, 95
BseDI CCNNGG 1 cut(s) 144
BseJI GATNNNNATC 1 cut(s) 51
BseLI CCNNNNNNNGG 2 cut(s) 229, 472
BseMII CTCAG 1 cut(s) 20
BseRI GAGGAG 1 cut(s) 281
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 1 cut(s) 392
Bsh1285I CGRYCG 1 cut(s) 95
BshVI ATCGAT 2 cut(s) 91, 95
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 592
BslFI GGGAC 2 cut(s) 10, 18
BslI CCNNNNNNNGG 2 cut(s) 229, 472
BsmFI GGGAC 2 cut(s) 10, 18
Bsp1286I GDGCHC 1 cut(s) 592
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 4 cut(s) 52, 88, 92, 445
Bsp19I CCATGG 1 cut(s) 144
BspCNI CTCAG 1 cut(s) 21
BspDI ATCGAT 2 cut(s) 91, 95
BspHI TCATGA 1 cut(s) 262
BspPI GGATC 1 cut(s) 440
BspTI CTTAAG 1 cut(s) 349
BsrGI TGTACA 1 cut(s) 573
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 4 cut(s) 52, 88, 92, 445
BssT1I CCWWGG 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 2 cut(s) 170, 400
BstAFI CTTAAG 1 cut(s) 349
BstAUI TGTACA 1 cut(s) 573
BstDEI CTNAG 1 cut(s) 29
BstDSI CCRYGG 1 cut(s) 144
BstKTI GATC 4 cut(s) 55, 91, 95, 448
BstMBI GATC 4 cut(s) 52, 88, 92, 445
BstMCI CGRYCG 1 cut(s) 95
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 1 cut(s) 187
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 1 cut(s) 392
BstV2I GAAGAC 1 cut(s) 253
Bsu15I ATCGAT 2 cut(s) 91, 95
BsuI GTATCC 1 cut(s) 185
BsuTUI ATCGAT 2 cut(s) 91, 95
BtgI CCRYGG 1 cut(s) 144
CciI TCATGA 1 cut(s) 262
Cfr13I GGNCC 2 cut(s) 185, 356
ClaI ATCGAT 2 cut(s) 91, 95
Csp6I GTAC 2 cut(s) 175, 574
CviAII CATG 5 cut(s) 22, 145, 263, 287, 546
CviJI RGCY 2 cut(s) 296, 563
CviKI_1 RGCY 2 cut(s) 296, 563
CviQI GTAC 2 cut(s) 175, 574
DdeI CTNAG 1 cut(s) 29
DpnI GATC 4 cut(s) 54, 90, 94, 447
DpnII GATC 4 cut(s) 52, 88, 92, 445
Eco130I CCWWGG 1 cut(s) 144
Eco47I GGWCC 2 cut(s) 185, 356
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 5 cut(s) 25, 148, 266, 290, 549
FaqI GGGAC 2 cut(s) 10, 18
FatI CATG 5 cut(s) 21, 144, 262, 286, 545
FauNDI CATATG 1 cut(s) 435
FblI GTMKAC 1 cut(s) 121
Fnu4HI GCNGC 1 cut(s) 406
Fsp4HI GCNGC 1 cut(s) 406
FspBI CTAG 1 cut(s) 297
GluI GCNGC 1 cut(s) 406
Hin1II CATG 5 cut(s) 25, 148, 266, 290, 549
HincII GTYRAC 1 cut(s) 441
HindII GTYRAC 1 cut(s) 441
HinfI GANTC 1 cut(s) 249
Hpy166II GTNNAC 5 cut(s) 122, 166, 177, 441, 590
Hpy188I TCNGA 1 cut(s) 232
Hpy188III TCNNGA 3 cut(s) 129, 253, 263
Hpy8I GTNNAC 5 cut(s) 122, 166, 177, 441, 590
HpyAV CCTTC 2 cut(s) 54, 423
HpyCH4III ACNGT 2 cut(s) 170, 400
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 3 cut(s) 200, 545, 590
HpyF3I CTNAG 1 cut(s) 29
HpySE526I ACGT 1 cut(s) 17
Hsp92II CATG 5 cut(s) 25, 148, 266, 290, 549
Kzo9I GATC 4 cut(s) 52, 88, 92, 445
LmnI GCTCC 1 cut(s) 342
LpnPI CCDG 4 cut(s) 41, 174, 201, 266
Lsp1109I GCAGC 1 cut(s) 392
LweI GCATC 2 cut(s) 209, 354
MaeI CTAG 1 cut(s) 297
MaeII ACGT 1 cut(s) 17
MaeIII GTNAC 2 cut(s) 152, 218
MalI GATC 4 cut(s) 54, 90, 94, 447
MboI GATC 4 cut(s) 52, 88, 92, 445
MboII GAAGA 2 cut(s) 258, 515
MhlI GDGCHC 1 cut(s) 592
MluCI AATT 3 cut(s) 372, 383, 479
MlyI GAGTC 1 cut(s) 243
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 2 cut(s) 259, 347
MseI TTAA 6 cut(s) 14, 134, 258, 330, 350, 375
MslI CAYNNNNRTG 2 cut(s) 535, 595
MspCI CTTAAG 1 cut(s) 349
MspR9I CCNGG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 189
NcoI CCATGG 1 cut(s) 144
NdeI CATATG 1 cut(s) 435
NdeII GATC 4 cut(s) 52, 88, 92, 445
NlaIII CATG 5 cut(s) 25, 148, 266, 290, 549
NspI RCATGY 1 cut(s) 290
OliI CACNNNNGTG 1 cut(s) 595
PagI TCATGA 1 cut(s) 262
PciI ACATGT 1 cut(s) 286
PcsI WCGNNNNNNNCGW 1 cut(s) 214
PfoI TCCNGGA 1 cut(s) 187
PkrI GCNGC 1 cut(s) 407
Ple19I CGATCG 1 cut(s) 95
PleI GAGTC 1 cut(s) 243
PpsI GAGTC 1 cut(s) 243
PscI ACATGT 1 cut(s) 286
PshBI ATTAAT 1 cut(s) 258
Psp1406I AACGTT 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspPI GGNCC 2 cut(s) 185, 356
PvuI CGATCG 1 cut(s) 95
RsaI GTAC 2 cut(s) 176, 575
RsaNI GTAC 2 cut(s) 175, 574
RseI CAYNNNNRTG 2 cut(s) 535, 595
SaqAI TTAA 6 cut(s) 14, 134, 258, 330, 350, 375
SatI GCNGC 1 cut(s) 406
Sau3AI GATC 4 cut(s) 52, 88, 92, 445
Sau96I GGNCC 2 cut(s) 185, 356
SchI GAGTC 1 cut(s) 243
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 1 cut(s) 592
SetI ASST 6 cut(s) 20, 162, 198, 298, 358, 529
SfaNI GCATC 2 cut(s) 209, 354
SinI GGWCC 2 cut(s) 185, 356
SmiMI CAYNNNNRTG 2 cut(s) 535, 595
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
Sse9I AATT 3 cut(s) 372, 383, 479
SspMI CTAG 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 170, 400
TaiI ACGT 1 cut(s) 20
TaqI TCGA 2 cut(s) 91, 95
TasI AATT 3 cut(s) 372, 383, 479
TatI WGTACW 1 cut(s) 573
Tru1I TTAA 6 cut(s) 14, 134, 258, 330, 350, 375
Tru9I TTAA 6 cut(s) 14, 134, 258, 330, 350, 375
TseI GCWGC 1 cut(s) 405
TspDTI ATGAA 5 cut(s) 10, 296, 474, 516, 581
Vha464I CTTAAG 1 cut(s) 349
VneI GTGCAC 1 cut(s) 588
VpaK11BI GGWCC 2 cut(s) 185, 356
VspI ATTAAT 1 cut(s) 258
XapI RAATTY 1 cut(s) 479
XceI RCATGY 1 cut(s) 290
XmiI GTMKAC 1 cut(s) 121
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.