Prupe.6G061200_v2.0.a1
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000468
Physical Location & Seq
Reverse (-)
4237915 .. 4240751
2837 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G061200.1

Sequence Viewer

Length: 417 bp
ATGGTCTCATGTTCGAATCCATATAACACATTGACAATTTGTGCGAAAGAACCCACCCCCTTTCTTAACTTTGGCACCAAAAAAAAAATTTCAAACTGGTTAAATTTAAAACGCGTGAAAAATTGGGTCTTATGCGTGATTCAAAGGTCCTTCAATGCAATCATGCATGGAGTGTTAAGATTACAAGATAGACTTTTGAAGGTGCCGGAACTCATACTTGATAATTGCACTGATAATAGATGGAGATGGTTTAAGAAACACTTGGGGGCGTTGGATGGGACTTACATTAGGGTTCGTGTCCCTAAACAAGATAAGCCAAGATATCGAACACAAAAGGGGGTGGTTACAACCAATGTATTGGGAGTTTGTGTTAATGTATGCATTTTGAGATCTGCTTCTAATTCTAGGGTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

139

Amino Acids

15.94

Weight (kDa)

10.31

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 74, 202
AccII CGCG 1 cut(s) 114
AcsI RAATTY 2 cut(s) 87, 103
AflIII ACRYGT 1 cut(s) 112
AgsI TTSAA 4 cut(s) 93, 143, 154, 199
Alw26I GTCTC 1 cut(s) 10
ApoI RAATTY 2 cut(s) 87, 103
AspS9I GGNCC 1 cut(s) 147
AsuII TTCGAA 1 cut(s) 14
AvaII GGWCC 1 cut(s) 147
BanI GGYRCC 2 cut(s) 74, 202
BccI CCATC 3 cut(s) 234, 240, 269
BcoDI GTCTC 1 cut(s) 10
BfaI CTAG 1 cut(s) 405
BglII AGATCT 1 cut(s) 389
Bme18I GGWCC 1 cut(s) 147
BmgT120I GGNCC 1 cut(s) 147
BmiI GGNNCC 2 cut(s) 76, 204
Bpu14I TTCGAA 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 10
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bse1I ACTGG 1 cut(s) 101
BseGI GGATG 1 cut(s) 280
BseNI ACTGG 1 cut(s) 101
Bsh1236I CGCG 1 cut(s) 114
BshNI GGYRCC 2 cut(s) 74, 202
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 2 cut(s) 284, 292
BsmAI GTCTC 1 cut(s) 10
BsmFI GGGAC 2 cut(s) 284, 292
Bso31I GGTCTC 1 cut(s) 10
Bsp119I TTCGAA 1 cut(s) 14
Bsp143I GATC 1 cut(s) 389
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 2 cut(s) 76, 204
BspT104I TTCGAA 1 cut(s) 14
BspT107I GGYRCC 2 cut(s) 74, 202
BspTNI GGTCTC 1 cut(s) 10
BsrI ACTGG 1 cut(s) 101
BssMI GATC 1 cut(s) 389
BstBI TTCGAA 1 cut(s) 14
BstF5I GGATG 1 cut(s) 280
BstFNI CGCG 1 cut(s) 114
BstKTI GATC 1 cut(s) 392
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 1 cut(s) 389
BstUI CGCG 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 389
BstXI CCANNNNNNTGG 1 cut(s) 358
BstYI RGATCY 1 cut(s) 389
BtsCI GGATG 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 147
CviAII CATG 3 cut(s) 9, 163, 167
CviJI RGCY 1 cut(s) 316
CviKI_1 RGCY 1 cut(s) 316
DpnI GATC 1 cut(s) 391
DpnII GATC 1 cut(s) 389
DraI TTTAAA 1 cut(s) 108
Eco31I GGTCTC 1 cut(s) 10
Eco32I GATATC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 147
EcoRV GATATC 1 cut(s) 323
EcoT22I ATGCAT 2 cut(s) 168, 383
FaeI CATG 3 cut(s) 12, 166, 170
FaiI YATR 9 cut(s) 10, 22, 24, 133, 164, 168, 215, 379, 415
FalI AAGNNNNNCTT 4 cut(s) 177, 209, 245, 277
FaqI GGGAC 2 cut(s) 284, 292
FatI CATG 3 cut(s) 8, 162, 166
FokI GGATG 1 cut(s) 287
FspBI CTAG 1 cut(s) 405
HapII CCGG 1 cut(s) 206
Hin1II CATG 3 cut(s) 12, 166, 170
HinfI GANTC 2 cut(s) 16, 139
HpaII CCGG 1 cut(s) 206
HpyAV CCTTC 2 cut(s) 160, 193
HpyCH4V TGCA 4 cut(s) 158, 166, 228, 381
Hsp92II CATG 3 cut(s) 12, 166, 170
Kzo9I GATC 1 cut(s) 389
LpnPI CCDG 2 cut(s) 82, 219
MaeI CTAG 1 cut(s) 405
MaeIII GTNAC 1 cut(s) 343
MalI GATC 1 cut(s) 391
MboI GATC 1 cut(s) 389
MflI RGATCY 1 cut(s) 389
MluCI AATT 6 cut(s) 36, 87, 103, 121, 223, 400
MluI ACGCGT 1 cut(s) 112
MmeI TCCRAC 1 cut(s) 252
Mph1103I ATGCAT 2 cut(s) 168, 383
MseI TTAA 6 cut(s) 66, 101, 107, 176, 252, 372
MspI CCGG 1 cut(s) 206
MvnI CGCG 1 cut(s) 114
NdeII GATC 1 cut(s) 389
NlaIII CATG 3 cut(s) 12, 166, 170
NlaIV GGNNCC 2 cut(s) 76, 204
NsiI ATGCAT 2 cut(s) 168, 383
NspV TTCGAA 1 cut(s) 14
PfeI GAWTC 2 cut(s) 16, 139
PpuMI RGGWCCY 1 cut(s) 147
Psp5II RGGWCCY 1 cut(s) 147
PspN4I GGNNCC 2 cut(s) 76, 204
PspPI GGNCC 1 cut(s) 147
PspPPI RGGWCCY 1 cut(s) 147
PsuI RGATCY 1 cut(s) 389
SaqAI TTAA 6 cut(s) 66, 101, 107, 176, 252, 372
Sau3AI GATC 1 cut(s) 389
Sau96I GGNCC 1 cut(s) 147
SetI ASST 2 cut(s) 149, 204
SfuI TTCGAA 1 cut(s) 14
SinI GGWCC 1 cut(s) 147
Sse9I AATT 6 cut(s) 36, 87, 103, 121, 223, 400
SspMI CTAG 1 cut(s) 405
TaqI TCGA 2 cut(s) 14, 325
TasI AATT 6 cut(s) 36, 87, 103, 121, 223, 400
TfiI GAWTC 2 cut(s) 16, 139
Tru1I TTAA 6 cut(s) 66, 101, 107, 176, 252, 372
Tru9I TTAA 6 cut(s) 66, 101, 107, 176, 252, 372
TscAI CASTG 1 cut(s) 235
TspRI CASTG 1 cut(s) 235
VpaK11BI GGWCC 1 cut(s) 147
XapI RAATTY 2 cut(s) 87, 103
XspI CTAG 1 cut(s) 405
Zsp2I ATGCAT 2 cut(s) 168, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.