pycom16g21630

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000468
Physical Location & Seq
Reverse (-)
20251803 .. 20252366
564 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g21630.1

Sequence Viewer

Length: 507 bp
ATGGTCTCAAGGTCCCAACTGTTATTATCAACTAATATAGGTACATATTATTTGGTTGATTCCGGATATACTAATGGTGAGGGTTTTCTAGCACCGCATAGAGGCACTAGATACCATTTGCAAGAGTGGGAAGACAATTCACGTGCACCTAGGAATCATGAAGAATATTTTAATATGAAACACTCACGTGCTAGGAATGCCATTGAGAGATGTTTTGGCCTCCTCAAACGACGTTGGGCTATCTTACGAAGTCCTTCCCACTATCCAATCAAGATTCACGGACAAATGATCACCGCATGTAGTTTACTTCATAATTTTATCAGAATGTATATGGCAATTGATCCTGAGGAAAATGCAAGGCTTGCATTTGATGAATTACCTATAGGGGAAGATTTACCAGAAGTATTAGCCTACATTGAAACCCTTGAGTCAAGCCAGATATGGACTCAATGGAGGGATGATCTTGCAAGAGAAATACATGATGAGTGGAGAGGAATAAGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

169

Amino Acids

19.8

Weight (kDa)

6.24

Isoelectric Point (pI)

65.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 3 - 105 1.7e-15 DDE superfamily endonuclease
DDE_Tnp_1 PF01609 13 - 100 1.8e-06 Transposase DDE domain
Plant_tran PF04827 48 - 107 2.5e-06 Plant transposon protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 62
AciI CCGC 2 cut(s) 95, 294
AclWI GGATC 1 cut(s) 335
AcvI CACGTG 2 cut(s) 143, 188
AfaI GTAC 1 cut(s) 43
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 1 cut(s) 419
AleI CACNNNNGTG 1 cut(s) 186
AluBI AGCT 1 cut(s) 503
AluI AGCT 1 cut(s) 503
Alw21I GWGCWC 1 cut(s) 148
Alw26I GTCTC 1 cut(s) 10
Alw44I GTGCAC 1 cut(s) 144
AlwI GGATC 1 cut(s) 335
Aor13HI TCCGGA 1 cut(s) 62
AoxI GGCC 1 cut(s) 217
ApaLI GTGCAC 1 cut(s) 144
Asp700I GAANNNNTTC 1 cut(s) 253
AspA2I CCTAGG 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 12
AsuHPI GGTGA 2 cut(s) 89, 283
AvaII GGWCC 1 cut(s) 12
AvrII CCTAGG 1 cut(s) 149
AxyI CCTNAGG 1 cut(s) 345
BaeGI GKGCMC 1 cut(s) 148
BbrPI CACGTG 2 cut(s) 143, 188
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 148
BclI TGATCA 1 cut(s) 288
BcoDI GTCTC 1 cut(s) 10
BfaI CTAG 4 cut(s) 89, 108, 150, 192
BfmI CTRYAG 1 cut(s) 381
BlnI CCTAGG 1 cut(s) 149
Bme18I GGWCC 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 12
BmiI GGNNCC 1 cut(s) 14
BpiI GAAGAC 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 446
BsaAI YACGTR 2 cut(s) 143, 188
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 1 cut(s) 149
BsaWI WCCGGW 1 cut(s) 62
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse21I CCTNAGG 1 cut(s) 345
BseAI TCCGGA 1 cut(s) 62
BseDI CCNNGG 1 cut(s) 149
BseGI GGATG 1 cut(s) 463
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMII CTCAG 1 cut(s) 336
BseRI GAGGAG 1 cut(s) 212
BseSI GKGCMC 1 cut(s) 148
BshFI GGCC 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 148
BsiSI CCGG 1 cut(s) 63
BslI CCNNNNNNNGG 1 cut(s) 101
BsmAI GTCTC 1 cut(s) 10
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 1 cut(s) 219
Bso31I GGTCTC 1 cut(s) 10
Bsp1286I GDGCHC 1 cut(s) 148
Bsp13I TCCGGA 1 cut(s) 62
Bsp143I GATC 3 cut(s) 288, 340, 460
BspACI CCGC 2 cut(s) 95, 294
BspANI GGCC 1 cut(s) 219
BspCNI CTCAG 1 cut(s) 337
BspEI TCCGGA 1 cut(s) 62
BspHI TCATGA 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 14
BspPI GGATC 1 cut(s) 335
BspTNI GGTCTC 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 3 cut(s) 288, 340, 460
BssT1I CCWWGG 1 cut(s) 149
Bst4CI ACNGT 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 362
BstBAI YACGTR 2 cut(s) 143, 188
BstC8I GCNNGC 1 cut(s) 363
BstDEI CTNAG 1 cut(s) 345
BstF5I GGATG 1 cut(s) 463
BstKTI GATC 3 cut(s) 291, 343, 463
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 3 cut(s) 288, 340, 460
BstMWI GCNNNNNNNGC 2 cut(s) 197, 362
BstNSI RCATGY 1 cut(s) 300
BstSFI CTRYAG 1 cut(s) 381
BstSLI GKGCMC 1 cut(s) 148
BstV2I GAAGAC 1 cut(s) 138
Bsu36I CCTNAGG 1 cut(s) 345
BsuRI GGCC 1 cut(s) 219
BtsCI GGATG 1 cut(s) 463
Cac8I GCNNGC 1 cut(s) 363
CciI TCATGA 1 cut(s) 157
Cfr13I GGNCC 1 cut(s) 12
Csp6I GTAC 1 cut(s) 42
CviAII CATG 3 cut(s) 158, 297, 479
CviJI RGCY 6 cut(s) 219, 239, 361, 410, 435, 503
CviKI_1 RGCY 6 cut(s) 219, 239, 361, 410, 435, 503
CviQI GTAC 1 cut(s) 42
DdeI CTNAG 1 cut(s) 345
DpnI GATC 3 cut(s) 290, 342, 462
DpnII GATC 3 cut(s) 288, 340, 460
Eco130I CCWWGG 1 cut(s) 149
Eco31I GGTCTC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 12
Eco72I CACGTG 2 cut(s) 143, 188
Eco81I CCTNAGG 1 cut(s) 345
EcoO109I RGGNCCY 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
FaeI CATG 3 cut(s) 161, 300, 482
FatI CATG 3 cut(s) 157, 296, 478
FbaI TGATCA 1 cut(s) 288
FokI GGATG 1 cut(s) 470
FspBI CTAG 4 cut(s) 89, 108, 150, 192
HaeIII GGCC 1 cut(s) 219
HapII CCGG 1 cut(s) 63
Hin1II CATG 3 cut(s) 161, 300, 482
HinfI GANTC 5 cut(s) 59, 154, 274, 428, 445
HpaII CCGG 1 cut(s) 63
HphI GGTGA 2 cut(s) 89, 283
Hpy166II GTNNAC 2 cut(s) 146, 305
Hpy188I TCNGA 1 cut(s) 323
Hpy188III TCNNGA 4 cut(s) 63, 158, 271, 344
Hpy8I GTNNAC 2 cut(s) 146, 305
Hpy99I CGWCG 1 cut(s) 234
HpyAV CCTTC 1 cut(s) 264
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4IV ACGT 3 cut(s) 142, 187, 232
HpyCH4V TGCA 5 cut(s) 121, 146, 356, 365, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 362
HpyF3I CTNAG 1 cut(s) 345
HpySE526I ACGT 3 cut(s) 142, 187, 232
Hsp92II CATG 3 cut(s) 161, 300, 482
Kpn2I TCCGGA 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 288
Kzo9I GATC 3 cut(s) 288, 340, 460
LpnPI CCDG 4 cut(s) 76, 357, 411, 449
MaeI CTAG 4 cut(s) 89, 108, 150, 192
MaeII ACGT 3 cut(s) 142, 187, 232
MalI GATC 3 cut(s) 290, 342, 462
MboI GATC 3 cut(s) 288, 340, 460
MboII GAAGA 3 cut(s) 143, 173, 401
MfeI CAATTG 1 cut(s) 336
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 4 cut(s) 136, 313, 336, 374
MlyI GAGTC 2 cut(s) 437, 439
MnlI CCTC 7 cut(s) 73, 95, 230, 233, 340, 447, 485
MroI TCCGGA 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 253
MseI TTAA 1 cut(s) 171
MslI CAYNNNNRTG 1 cut(s) 186
MspI CCGG 1 cut(s) 63
MunI CAATTG 1 cut(s) 336
Mva1269I GAATGC 1 cut(s) 202
MwoI GCNNNNNNNGC 2 cut(s) 197, 362
NdeII GATC 3 cut(s) 288, 340, 460
NlaIII CATG 3 cut(s) 161, 300, 482
NlaIV GGNNCC 1 cut(s) 14
NspI RCATGY 1 cut(s) 300
OliI CACNNNNGTG 1 cut(s) 186
PagI TCATGA 1 cut(s) 157
PctI GAATGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 253
PfeI GAWTC 3 cut(s) 59, 154, 274
PleI GAGTC 2 cut(s) 436, 439
PmaCI CACGTG 2 cut(s) 143, 188
PmlI CACGTG 2 cut(s) 143, 188
PpsI GAGTC 2 cut(s) 436, 439
Ppu21I YACGTR 2 cut(s) 143, 188
PpuMI RGGWCCY 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 12
PspCI CACGTG 2 cut(s) 143, 188
PspN4I GGNNCC 1 cut(s) 14
PspPI GGNCC 1 cut(s) 12
PspPPI RGGWCCY 1 cut(s) 12
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
RseI CAYNNNNRTG 1 cut(s) 186
SaqAI TTAA 1 cut(s) 171
Sau3AI GATC 3 cut(s) 288, 340, 460
Sau96I GGNCC 1 cut(s) 12
SchI GAGTC 2 cut(s) 437, 439
SduI GDGCHC 1 cut(s) 148
SetI ASST 8 cut(s) 14, 43, 145, 151, 190, 235, 382, 505
SfcI CTRYAG 1 cut(s) 381
SinI GGWCC 1 cut(s) 12
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 2 cut(s) 7, 425
SmoI CTYRAG 2 cut(s) 7, 425
Sse9I AATT 4 cut(s) 136, 313, 336, 374
SsiI CCGC 2 cut(s) 95, 294
SspI AATATT 1 cut(s) 167
SspMI CTAG 4 cut(s) 89, 108, 150, 192
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 1 cut(s) 21
TaiI ACGT 3 cut(s) 145, 190, 235
TasI AATT 4 cut(s) 136, 313, 336, 374
TfiI GAWTC 3 cut(s) 59, 154, 274
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TspDTI ATGAA 4 cut(s) 174, 191, 299, 387
TspGWI ACGGA 1 cut(s) 294
VneI GTGCAC 1 cut(s) 144
VpaK11BI GGWCC 1 cut(s) 12
XceI RCATGY 1 cut(s) 300
XmaJI CCTAGG 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 253
XspI CTAG 4 cut(s) 89, 108, 150, 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.