FvH4_6g09143
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000468
Physical Location & Seq
Forward (+)
5430499 .. 5431034
536 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09143.t1

Sequence Viewer

Length: 399 bp
ATGGATAGAAGGACTTTTGGGTTGTTATGTGAACTGCTCCGCACTGATGGAAGGCTAAAAAATGATGGATTAGTTACTGTGGAGGGGCAAGTTTATATGTTTCTACACATACTTGCTCATCATGTTAAGAATCGTACCATTAGAGGTAGATTCTTCCAATCAGGAGAGACAGTTAGTAGGTATTTCAACTGTGTATTGCAAGGTGTCTTACGATTACAAGCCAATCTATTGAGAATGCCAGACCCCAATTGTTTGGGAGCTTTAGATGGAACTTATGTTAGAGTGCGTGTAGCTGCTACTGACAAGGCAAGATACCGAACTAGAAAGGCAGATATTGCAACAAATGTCTTGGCAGCTTGTTTGCGTGACATGCAGTTCACATTCGTGTTACCGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

133

Amino Acids

15.02

Weight (kDa)

10.04

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 1 - 70 7.4e-18 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000468)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g04030 FvH4_2g09411 FvH4_3g15551 FvH4_3g36421 FvH4_4g11701 FvH4_5g36381 FvH4_6g09143 FvH4_6g09144 FvH4_6g21972
malus_domestica MD00G1096500.v1.1 MD00G1122500.v1.1 MD02G1090600.v1.1 MD02G1173300.v1.1 MD05G1202800.v1.1 MD14G1123600.v1.1
prunus_persica Prupe.1G083600_v2.0.a1 Prupe.1G245300_v2.0.a1 Prupe.5G002100_v2.0.a1 Prupe.6G061200_v2.0.a1
pyrus_communis pycom01g07060 pycom02g13920 pycom04g11780 pycom04g13480 pycom04g21940 pycom06g07950 pycom07g03740 pycom07g03980 pycom09g02010 pycom09g02020 pycom09g11510 pycom09g14260 pycom10g02500 pycom10g15690 pycom12g08730 pycom12g08740 pycom14g19840 pycom15g16780 pycom15g24940 pycom15g25660 pycom16g21630 pycom16g26320
rosa_chinensis RchiOBHm_Chr1g0330371 RchiOBHm_Chr1g0331991 RchiOBHm_Chr6g0300321
rosa_laevigata RLG00000013795
rosa_multiflora Rmu_sc0000060.1_g000010 Rmu_sc0000288.1_g000044 Rmu_sc0002219.1_g000002 Rmu_sc0003995.1_g000003
rosa_roxburghii Rroxscaffold_1G00059020 Rroxscaffold_2G00108450 Rroxscaffold_2G00112460 Rroxscaffold_5G00375700 Rroxscaffold_5G00381640 Rroxscaffold_7G00158590
rosa_rugosa Rorug01G0237300 Rorug01G0237400 Rorug01G0237500 Rorug02G0051900 Rorug03G0260600 Rorug03G0285800 Rorug04G0076800 Rorug04G0162600 Rorug07G0264500 Rorug07G0264500
rosa_samantha Rh2DG003500 Rh3CG244800 Rh4CG334200 Rh5AG318300 Rh5BG549000 Rh7AG126400 Rh7CG118500 Rh7CG154500 Rh7DG025400
rosa_wichuraiana Rw1G026390 Rw2G005400 Rw2G034490 Rw3G019530 Rw3G028000 Rw4G010490 Rw5G010830 Rw5G023340 Rw5G026580 Rw5G041150 Rw6G003010 Rw6G003290 Rw6G010630 Rw6G021220 Rw6G035650 Rw7G014310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 40
AfaI GTAC 1 cut(s) 136
AgsI TTSAA 1 cut(s) 187
AleI CACNNNNGTG 1 cut(s) 383
AluBI AGCT 3 cut(s) 260, 293, 356
AluI AGCT 3 cut(s) 260, 293, 356
Alw26I GTCTC 1 cut(s) 161
ApeKI GCWGC 2 cut(s) 293, 353
AsuC2I CCSGG 1 cut(s) 393
BbvI GCAGC 2 cut(s) 280, 365
BccI CCATC 3 cut(s) 41, 59, 260
BcnI CCSGG 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 1 cut(s) 321
BisI GCNGC 2 cut(s) 294, 354
BlsI GCNGC 2 cut(s) 295, 355
Bme1390I CCNGG 1 cut(s) 393
BmrFI CCNGG 1 cut(s) 393
BpuMI CCSGG 1 cut(s) 393
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
BseXI GCAGC 2 cut(s) 280, 365
BsiSI CCGG 1 cut(s) 392
BsmAI GTCTC 1 cut(s) 161
BsmI GAATGC 1 cut(s) 240
BspACI CCGC 1 cut(s) 40
Bst4CI ACNGT 3 cut(s) 79, 172, 191
BstAPI GCANNNNNTGC 1 cut(s) 335
BstMAI GTCTC 1 cut(s) 161
BstMWI GCNNNNNNNGC 2 cut(s) 335, 370
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 1 cut(s) 391
BstV1I GCAGC 2 cut(s) 280, 365
BstXI CCANNNNNNTGG 1 cut(s) 253
BtsIMutI CAGTG 1 cut(s) 42
Csp6I GTAC 1 cut(s) 135
CviAII CATG 2 cut(s) 122, 370
CviJI RGCY 5 cut(s) 55, 221, 260, 293, 356
CviKI_1 RGCY 5 cut(s) 55, 221, 260, 293, 356
CviQI GTAC 1 cut(s) 135
FaeI CATG 2 cut(s) 125, 373
FaiI YATR 7 cut(s) 28, 96, 98, 110, 123, 276, 371
FatI CATG 2 cut(s) 121, 369
Fnu4HI GCNGC 2 cut(s) 294, 354
Fsp4HI GCNGC 2 cut(s) 294, 354
FspBI CTAG 1 cut(s) 321
GluI GCNGC 2 cut(s) 294, 354
HapII CCGG 1 cut(s) 392
Hin1II CATG 2 cut(s) 125, 373
HinfI GANTC 2 cut(s) 130, 150
HpaII CCGG 1 cut(s) 392
Hpy166II GTNNAC 2 cut(s) 32, 378
Hpy188III TCNNGA 1 cut(s) 162
Hpy8I GTNNAC 2 cut(s) 32, 378
HpyAV CCTTC 2 cut(s) 3, 45
HpyCH4III ACNGT 3 cut(s) 79, 172, 191
HpyCH4V TGCA 3 cut(s) 199, 338, 373
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 370
Hsp92II CATG 2 cut(s) 125, 373
LmnI GCTCC 2 cut(s) 42, 257
LpnPI CCDG 2 cut(s) 147, 252
Lsp1109I GCAGC 2 cut(s) 280, 365
MaeI CTAG 1 cut(s) 321
MaeIII GTNAC 3 cut(s) 73, 365, 387
MboII GAAGA 1 cut(s) 145
MfeI CAATTG 1 cut(s) 247
MluCI AATT 1 cut(s) 247
MnlI CCTC 2 cut(s) 76, 137
MseI TTAA 1 cut(s) 126
MslI CAYNNNNRTG 1 cut(s) 383
MspI CCGG 1 cut(s) 392
MspR9I CCNGG 1 cut(s) 393
MunI CAATTG 1 cut(s) 247
Mva1269I GAATGC 1 cut(s) 240
MwoI GCNNNNNNNGC 2 cut(s) 335, 370
NciI CCSGG 1 cut(s) 393
NlaIII CATG 2 cut(s) 125, 373
NmuCI GTSAC 1 cut(s) 365
NspI RCATGY 1 cut(s) 373
OliI CACNNNNGTG 1 cut(s) 383
PctI GAATGC 1 cut(s) 240
PfeI GAWTC 2 cut(s) 130, 150
PkrI GCNGC 2 cut(s) 295, 355
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
RseI CAYNNNNRTG 1 cut(s) 383
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 2 cut(s) 294, 354
ScrFI CCNGG 1 cut(s) 393
SetI ASST 6 cut(s) 148, 182, 205, 262, 295, 358
SmiMI CAYNNNNRTG 1 cut(s) 383
Sse9I AATT 1 cut(s) 247
SsiI CCGC 1 cut(s) 40
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 1 cut(s) 391
TaaI ACNGT 3 cut(s) 79, 172, 191
TasI AATT 1 cut(s) 247
TfiI GAWTC 2 cut(s) 130, 150
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 1 cut(s) 49
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 2 cut(s) 293, 353
Tsp45I GTSAC 1 cut(s) 365
TspRI CASTG 1 cut(s) 49
XceI RCATGY 1 cut(s) 373
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.